Fix CRAMReferenceRegion updating.

src/main/java/htsjdk/samtools/cram/build/CRAMReferenceRegion.java

@@ -39,6 +39,8 @@
  * {@link #fetchReferenceBases(int)} or {@link #fetchReferenceBasesByRegion(int, int, int)}. It caches the bases
  * from the previous request, along with metadata about the (0-based) start offset, and length of the
  * cached bases.
+ *
+ * NOTE: this class is not thread-safe/safe for concurrent access across threads.
  */
 public class CRAMReferenceRegion {
     private static final Log log = Log.getInstance(CRAMReferenceRegion.class);
@@ -113,17 +115,18 @@ public void fetchReferenceBases(final int referenceIndex) {
 
         // Re-resolve the reference bases if we don't have a current region or if the region we have
         // doesn't span the *entire* contig requested.
+        final SAMSequenceRecord newSequenceRecord = getSAMSequenceRecord(referenceIndex);
         if ((referenceIndex != this.referenceIndex) ||
                 regionStart != 0 ||
-                (regionLength < referenceBases.length)) {
+                (regionLength != newSequenceRecord.getSequenceLength())) {
             setCurrentSequence(referenceIndex);
-            referenceBases = referenceSource.getReferenceBases(sequenceRecord, true);
+            referenceBases = referenceSource.getReferenceBases(newSequenceRecord, true);
             if (referenceBases == null) {
                 throw new IllegalArgumentException(
-                        String.format("A reference must be supplied (reference sequence %s not found).", sequenceRecord));
+                        String.format("A reference must be supplied (reference sequence %s not found).", newSequenceRecord));
             }
             regionStart = 0;
-            regionLength = sequenceRecord.getSequenceLength();
+            regionLength = newSequenceRecord.getSequenceLength();
         }
     }
 

src/test/java/htsjdk/samtools/CRAMAllEncodingStrategiesTest.java

@@ -10,43 +10,104 @@
 import htsjdk.samtools.util.Tuple;
 import htsjdk.utils.SamtoolsTestUtils;
 import org.testng.Assert;
+import org.testng.SkipException;
 import org.testng.annotations.DataProvider;
 import org.testng.annotations.Test;
 
 import java.io.*;
 import java.util.*;
 
+/**
+ * Test roundtripping files through the GATK writer using both the default HTSJDK encoding strategy, plus a variety
+ * of alternative encoding strategies, in order to stress test the writer implementation. Compares the results with
+ * the original file, and then roundtrips the newly written CRAM through the samtools writer, validating that samtools
+ * can consume the HTSJDK-written files with the expected level of roundtrip fidelity (CRAMs don't always roundtrip
+ * with complete bit-level fidelity, i.e, samtools will resurrect NM/MD tags whether they were present in the original
+ * file or not unless they are specifically excluded, etc.). So in some case, you can't use full SAMRecord comparisons,
+ * in which case we fall back to lenient equality and restrict the comparison to read names, bases, alignment start/stop,
+ * and quality scores.
+ */
 public class CRAMAllEncodingStrategiesTest extends HtsjdkTest {
 
     private static final File TEST_DATA_DIR = new File("src/test/resources/htsjdk/samtools/cram");
     private final CompressorCache compressorCache = new CompressorCache();
 
-    @DataProvider(name="roundTripTestFiles")
-    public Object[][] roundTripTestFiles() {
+    @DataProvider(name="defaultStrategyRoundTripTestFiles")
+    public Object[][] defaultStrategyRoundTripTestFiles() {
         return new Object[][] {
+                // a test file with artificially small slices and containers to force multiple slices and containers
                 { new File(TEST_DATA_DIR, "NA12878.20.21.1-100.100-SeqsPerSlice.500-unMapped.cram"),
-                        new File(TEST_DATA_DIR, "human_g1k_v37.20.21.1-100.fasta") },
+                        new File(TEST_DATA_DIR, "human_g1k_v37.20.21.1-100.fasta"),
+                        false, false },
+                // the same file without the artificially small container constraints
+                { new File(TEST_DATA_DIR, "CEUTrio.HiSeq.WGS.b37.NA12878.20.21.10m-10m100.cram"),
+                        new File("src/test/resources/htsjdk/samtools/reference/human_g1k_v37.20.21.fasta.gz"),
+                        false, false },
+                // a test file with only unmapped reads
+                { new File(TEST_DATA_DIR, "NA12878.unmapped.cram"),
+                        new File(TEST_DATA_DIR, "human_g1k_v37.20.21.1-100.fasta"),
+                        false, false },
+                // generated with samtools 1.19 from the gatk bam file CEUTrio.HiSeq.WGS.b37.NA12878.20.21.bam
+                { new File(TEST_DATA_DIR, "CEUTrio.HiSeq.WGS.b37.NA12878.20.21.v3.0.samtools.cram"),
+                        new File("src/test/resources/htsjdk/samtools/reference/human_g1k_v37.20.21.fasta.gz"),
+                        true, false },
+
+                // these tests use lenient equality to only validate read names, bases, alignment start/stop, and qual scores
+
+                // a user-contributed file with reads aligned only to the mito contig that has been rewritten (long ago) with GATK
+                { new File(TEST_DATA_DIR, "mitoAlignmentStartTestGATKGen.cram"),
+                        new File(TEST_DATA_DIR, "mitoAlignmentStartTest.fa"), true, false },
+                // the original user-contributed file with reads aligned only to the mito contig
+                { new File(TEST_DATA_DIR, "mitoAlignmentStartTest.cram"),
+                        new File(TEST_DATA_DIR, "mitoAlignmentStartTest.fa"), true, false },
+                // files created by rewriting the htsjdk test file src/test/resources/htsjdk/samtools/cram/mitoAlignmentStartTest.cram
+                // using code that replicates the first read (which is aligned to position 1 of the mito contig) either
+                // 10,000 or 20,000 times, to create a file with 2 or 3 containers, respectively, that have reads aligned to
+                // position 1 of the contig
+                { new File(TEST_DATA_DIR, "mitoAlignmentStartTest_2_containers_aligned_to_pos_1.cram"),
+                        new File(TEST_DATA_DIR, "mitoAlignmentStartTest.fa"), true, false },
+                { new File(TEST_DATA_DIR, "mitoAlignmentStartTest_3_containers_aligned_to_pos_1.cram"),
+                        new File(TEST_DATA_DIR, "mitoAlignmentStartTest.fa"), true, false }
         };
     }
 
-    @Test(dataProvider = "roundTripTestFiles")
-    public final void testRoundTripDefaultEncodingStrategy(final File sourceFile, final File referenceFile) throws IOException {
+    @Test(dataProvider = "defaultStrategyRoundTripTestFiles")
+    public final void testRoundTripDefaultEncodingStrategy(
+            final File sourceFile,
+            final File referenceFile,
+            final boolean lenientEquality,
+            final boolean emitDetail) throws IOException {
+        // test the default encoding strategy
         final CRAMEncodingStrategy testStrategy = new CRAMEncodingStrategy();
         final File tempOutCRAM = File.createTempFile("testRoundTrip", ".cram");
         tempOutCRAM.deleteOnExit();
         CRAMTestUtils.writeToCRAMWithEncodingStrategy(testStrategy, sourceFile, tempOutCRAM, referenceFile);
-        assertRoundTripFidelity(sourceFile, tempOutCRAM, referenceFile, false);
-        assertRoundtripFidelityWithSamtools(tempOutCRAM, referenceFile);
+        assertRoundTripFidelity(sourceFile, tempOutCRAM, referenceFile, lenientEquality, emitDetail);
+        // test interop with samtools using this encoding
+        assertRoundtripFidelityWithSamtools(tempOutCRAM, referenceFile, lenientEquality, emitDetail);
+    }
+
+    @DataProvider(name="encodingStrategiesTestFiles")
+    public Object[][] encodingStrategiesTestFiles() {
+        return new Object[][] {
+                { new File(TEST_DATA_DIR, "NA12878.20.21.1-100.100-SeqsPerSlice.500-unMapped.cram"),
+                        new File(TEST_DATA_DIR, "human_g1k_v37.20.21.1-100.fasta"), false, false },
+        };
     }
 
-    @Test(dataProvider = "roundTripTestFiles")
-    public final void testAllEncodingStrategyCombinations(final File cramSourceFile, final File referenceFile) throws IOException {
+    @Test(dataProvider = "encodingStrategiesTestFiles")
+    public final void testAllEncodingStrategyCombinations(
+            final File cramSourceFile,
+            final File referenceFile,
+            final boolean lenientEquality,
+            final boolean emitDetail) throws IOException {
         for (final Tuple<String, CRAMEncodingStrategy> testStrategy : getAllEncodingStrategies()) {
             final File tempOutCRAM = File.createTempFile("allEncodingStrategyCombinations", ".cram");
             tempOutCRAM.deleteOnExit();
             CRAMTestUtils.writeToCRAMWithEncodingStrategy(testStrategy.b, cramSourceFile, tempOutCRAM, referenceFile);
-            assertRoundTripFidelity(cramSourceFile, tempOutCRAM, referenceFile, false);
-            assertRoundtripFidelityWithSamtools(tempOutCRAM, referenceFile);
+            assertRoundTripFidelity(cramSourceFile, tempOutCRAM, referenceFile, lenientEquality, emitDetail);
+            // test interop with samtools using this encoding
+            assertRoundtripFidelityWithSamtools(tempOutCRAM, referenceFile, lenientEquality, emitDetail);
         }
     }
 
@@ -82,6 +143,7 @@ public void assertRoundTripFidelity(
             final File sourceFile,
             final File targetCRAMFile,
             final File referenceFile,
+            final boolean lenientEquality,
             final boolean emitDetail) throws IOException {
         try (final SamReader sourceReader = SamReaderFactory.makeDefault()
                 .referenceSequence(referenceFile)
@@ -91,7 +153,15 @@ public void assertRoundTripFidelity(
             final SAMRecordIterator sourceIterator = sourceReader.iterator();
             final SAMRecordIterator targetIterator = copyReader.getIterator();
             while (sourceIterator.hasNext() && targetIterator.hasNext()) {
-                if (emitDetail) {
+                if (lenientEquality) {
+                    final SAMRecord sourceRec = sourceIterator.next();
+                    final SAMRecord targetRec = targetIterator.next();
+                    Assert.assertEquals(targetRec.getReadName(), sourceRec.getReadName());
+                    Assert.assertEquals(targetRec.getAlignmentStart(), sourceRec.getAlignmentStart());
+                    Assert.assertEquals(targetRec.getAlignmentEnd(), sourceRec.getAlignmentEnd());
+                    Assert.assertEquals(targetRec.getReadBases(), sourceRec.getReadBases());
+                    Assert.assertEquals(targetRec.getBaseQualities(), sourceRec.getBaseQualities());
+                } else if (emitDetail) {
                     final SAMRecord sourceRec = sourceIterator.next();
                     final SAMRecord targetRec = targetIterator.next();
                     if (!sourceRec.equals(targetRec)) {
@@ -108,13 +178,19 @@ public void assertRoundTripFidelity(
         }
     }
 
-    private void assertRoundtripFidelityWithSamtools(final File sourceCRAM, final File referenceFile) throws IOException {
+    private void assertRoundtripFidelityWithSamtools(
+            final File sourceCRAM,
+            final File referenceFile,
+            final boolean lenientEquality,
+            final boolean emitDetail) throws IOException {
         if (SamtoolsTestUtils.isSamtoolsAvailable()) {
             final File samtoolsOutFile = SamtoolsTestUtils.convertToCRAM(
                     sourceCRAM,
                     referenceFile,
                     "--input-fmt-option decode_md=0 --output-fmt-option store_md=0 --output-fmt-option store_nm=0");
-            assertRoundTripFidelity(sourceCRAM, samtoolsOutFile, referenceFile, false);
+            assertRoundTripFidelity(sourceCRAM, samtoolsOutFile, referenceFile, lenientEquality, emitDetail);
+        } else {
+            throw new SkipException("samtools is not installed, skipping test");
         }
     }
 

src/test/java/htsjdk/samtools/cram/ref/CRAMReferenceRegionTest.java

@@ -3,6 +3,7 @@
 import htsjdk.HtsjdkTest;
 import htsjdk.samtools.SAMSequenceRecord;
 import htsjdk.samtools.cram.build.CRAMReferenceRegion;
+import htsjdk.samtools.cram.structure.AlignmentContext;
 import htsjdk.samtools.cram.structure.CRAMStructureTestHelper;
 import htsjdk.samtools.reference.InMemoryReferenceSequenceFile;
 import org.testng.Assert;
@@ -167,6 +168,44 @@ public void testGetReferenceBasesByRegionPositive(
         Assert.assertEquals(bases, Arrays.copyOfRange(fullContigBases, requestedOffset, requestedOffset + requestedLength));
     }
 
+    // Simulate the state transitions that occur when writing a CRAM file; specifically, use transitions that mirror
+    // the ones that occur when writing a CRAM with the conditions that affect (and that fail without the fix to)
+    // https://github.com/broadinstitute/gatk/issues/8768, i.e., a container with one or more containers with reads
+    // aligned to position 1 of a given contig, followed by one or more containers with reads aligned past position 1
+    // of the same contig.
+    @Test
+    public void testSerialStateTransitions() {
+        // start with an entire reference sequence
+        final CRAMReferenceRegion cramReferenceRegion = getAlternatingReferenceRegion();
+        cramReferenceRegion.fetchReferenceBases(CRAMStructureTestHelper.REFERENCE_SEQUENCE_ZERO);
+        final long fullRegionFragmentLength = cramReferenceRegion.getRegionLength();
+        Assert.assertEquals(fullRegionFragmentLength, CRAMStructureTestHelper.REFERENCE_CONTIG_LENGTH);
+
+        // transition to a shorter reference fragment using fetchReferenceBasesByRegion, then back to the full region
+        final int SHORT_FRAGMENT_LENGTH = 5;
+        Assert.assertTrue(SHORT_FRAGMENT_LENGTH < fullRegionFragmentLength);
+        cramReferenceRegion.fetchReferenceBasesByRegion(CRAMStructureTestHelper.REFERENCE_SEQUENCE_ZERO, 0, SHORT_FRAGMENT_LENGTH);
+        Assert.assertEquals(cramReferenceRegion.getRegionLength(), SHORT_FRAGMENT_LENGTH);
+
+        // now transition back to the full sequence; this is where the bug previously would have occurred
+        cramReferenceRegion.fetchReferenceBases(CRAMStructureTestHelper.REFERENCE_SEQUENCE_ZERO);
+        // this assert would fail without the fix
+        Assert.assertEquals(cramReferenceRegion.getRegionLength(), fullRegionFragmentLength);
+
+        // transition to a shorter region fragment length using fetchReferenceBasesByRegion(AlignmentContext), then back to the full region
+        Assert.assertTrue(SHORT_FRAGMENT_LENGTH < fullRegionFragmentLength);
+        cramReferenceRegion.fetchReferenceBasesByRegion(
+                new AlignmentContext(
+                        new ReferenceContext(CRAMStructureTestHelper.REFERENCE_SEQUENCE_ZERO),
+                        1,
+                        SHORT_FRAGMENT_LENGTH));
+        Assert.assertEquals(cramReferenceRegion.getRegionLength(), SHORT_FRAGMENT_LENGTH);
+
+        // now transition back to the full sequence
+        cramReferenceRegion.fetchReferenceBases(CRAMStructureTestHelper.REFERENCE_SEQUENCE_ZERO);
+        Assert.assertEquals(cramReferenceRegion.getRegionLength(), fullRegionFragmentLength);
+    }
+
     @Test
     public void testGetReferenceBasesByRegionExceedsContigLength() {
         int regionStart = CRAMStructureTestHelper.REFERENCE_CONTIG_LENGTH / 2;