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Update parameters description
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mobinasri authored Dec 3, 2024
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4 changes: 2 additions & 2 deletions wdls/workflows/long_read_aligner_scattered.wdl
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Expand Up @@ -21,8 +21,8 @@ workflow longReadAlignmentScattered {
readfiles: "An array of read files. Their format can be either fastq, fq, fastq.gz, fq.gz, bam or cram. For cram format referenceFastaForReadExtraction should also be passed."
assemblyFasta: "Path to uncompressed or gzip-compressed fasta file of the diploid or haploid assembly"
aligner: "Name of the aligner. It can be either minimap2, winnowmap or veritymap. (Default = winnowmap)"
preset: "Paremeter preset should be selected based on aligner and sequencing platform. Common presets are map-pb/map-hifi/map-ont for minimap2, map-pb/map-ont for winnowmap and hifi-haploid/hifi-haploid-complete/hifi-diploid/ont-haploid-complete for veritymap"
kmerSize: "The kmer size for using minimap2 or winnowmap. With winnowmap kmer size should be 15 and with minimap2 kmer size should be 17 and 19 for using the presets map-ont and map-hifi/map-pb respectively."
preset: "(Required) Paremeter preset should be selected based on aligner and sequencing platform. Common presets are lr:hqae/map-ont for minimap2, map-pb/map-ont for winnowmap and hifi-haploid/hifi-haploid-complete/hifi-diploid/ont-haploid-complete for veritymap"
kmerSize: "The kmer size for using minimap2 or winnowmap. With winnowmap kmer size should be 15 and with minimap2 kmer size should be 15 and 25 for using the presets map-ont and lr:hqae respectively."
alignerOptions: "Aligner options. It can be something like '--eqx --cs -Y -L -y' for minimap2/winnowmap. Note that if assembly is diploid and aligner is either minimap2 or winnowmap '-I8g' is necessary. If the reads contain modification tags and these tags are supposed to be present in the final alignment file, alignerOptions should contain '-y' and the aligner should be either minimap2 or winnowmap. If running secphase is enabled it is recommended to add '-p0.5' to alignerOptions; it will keep more secondary alignments so secphase will have more candidates per read. For veritymap '--careful' can be used but not recommended for whole-genome assembly since it increases the runtime dramatically."
readExtractionOptions: "The options to be used while converting bam to fastq with samtools fastq. If the reads contain epigentic modification tags it is necessary to use '-TMm,Ml'"
sampleName: "Name of the sample or assembly. For example 'HG002', 'GRCh38' or 'HG002_hifiasm_v0.19.5'"
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