Releases: metagenome-atlas/atlas
GTDB V9 R220, Spades 4
v2.18.2
All in the sample table
What's Changed
- Qc reads, assembly are now written in the sample.tsv from the start. This should fix errors of partial writing to the sample.tsv #695
- It also allows you to add external assemblies.
- singletons reads are no longer used trough the pipeline.
- This changes the default paths for raw reads and assemblies.
assembly are now inAssembly/fasta/{sample}.fasta
reads:QC/reads/{sample}_{fraction}.fastq.gz
Seemless update: If you update atlas and continue on an old project. Your old files will be copies. Or the path defined in the sample.tsv will be used.
Co-binning
Co-binning with sub-groups
In this new version, Atlas uses binning with co-abundance as default.
While binning each sample individually is faster, using co-abundance for binning, by quantifying the coverage of contigs across multiple samples provides valuable insights about contig co-variation.
Starting with version 2.18, atlas places every sample in a single BinGroup and defaults to vamb as the binner unless there are very few samples. For fewer than 8 samples, metabat is the default binner.
The defaults are fine except when you have many samples (>150) where atlas gives a warning that you should put sour samples in more than one bin group.
Note
Previously each sample was put in its own BinGroup optimized for single-sample binning.
Running vamb in those versions would consider all samples, regardless of their BinGroup.
Hence updating to v2.18 might cause errors if using a sample.tsv file from an older Atlas version.
You can resolve this by assigning a unique BinGroup to each sample.
Full Changelog: v2.17.2...v2.18.0
v2.17.2
Fixes
- Ignore certificate for gtdb_v08 by @mladen5000 in #674
- Fixed pandas dependency for instrain by @mladen5000 in #678
- Convert mem_mb value from gb to mb for select rules by @LLansing in #681
- ci: use micromamba by @SilasK in #682
Contributors
Assets 2
Use skani for genome clustering
Skani
The tool Skani claims to be better and faster than the combination of mash + FastANI as used by dRep
I implemented the skin for species clustering.
We now do the species clustering in the atlas run binning step.
So you get information about the number of dereplicated species in the binning report. This allows you to run different binners before choosing the one to use for the genome annotation.
Also, the file storage was improved all important files are in Binning/{binner}/
My custom species clustering does the following steps:
- Pre-cluster genomes with single-linkage at 92.5 ANI.
- Re-calibrate checkm2 results.
- If a minority of genomes from a pre-cluster use a different translation table they are removed
- If some genomes of a pre-cluster don't use the specialed completeness model we re-calibrate completeness to the minimum value.
This ensures that not a bad genome evaluated on the general model is preferred over a better genome evaluated on the specific model.
See also https://silask.github.io/post/better_genomes/ Section 2. - Drop genomes that don't correspond to the filter criteria after re-calibration
- Cluster genomes with ANI threshold default 95%
- Select the best genome as representative based on the Quality score Completeness - 5x Contamination
New Contributors
Full Changelog: v2.16.3...v2.17.0
GTDB v8
V2.16
Contributors
Assets 2
v2.15.2
What's Changed
- Annotate gene catalog with Kegg, CAZy using DRAM
- You can turn off GUNC
Full Changelog: v2.15.1...v2.15.2
GUNC'n'More
What's Changed
- Use Gunc
- New Folder organisation: Main output files for Binning are in the new folder
Binning - Use hdf-format for gene catalogs. Allow efficient storage and selective access to large count and coverage matrices from the genecatalog. (See docs for how to load them) #621
- Semibin v. 1.5 by @SilasK in #622