SigProfilerPlotting provides a standard tool for displaying all types of mutational signatures as well as all types of mutational patterns in cancer genomes. The tool seamlessly integrates with other SigProfiler tools.
INTRODUCTION
The purpose of this document is to provide a guide for using the SigProfilerPlotting framework and associated functions/tools to visualize the output from SigProfilerExtraction and SigProfilerSimulator. An extensive Wiki page detailing the usage of this tool can be found at https://osf.io/2aj6t/wiki/home.
For users that prefer working in an R environment, a wrapper package is provided and can be found and installed from: https://github.com/AlexandrovLab/SigProfilerPlottingR
PREREQUISITES
The framework is written in PYTHON, however, it also requires the following software with the given versions (or newer):
- PYTHON version 3.4 or newer
- SigProfilerMatrixGenerator (recommended)
QUICK START GUIDE
This section will guide you through the minimum steps required to plot mutational matrices:
- Install the python package using pip:
pip install SigProfilerPlotting
- From within a python session, you can now plot your mutational matrices as follows:
$ python3
>> import sigProfilerPlotting as sigPlt
>> sigPlt.plotSBS(matrix_path, output_path, project, plot_type, percentage=False)
The available functions are listed below and the layout of the required parameters are as follows:
sigPlt.plotSBS(matrix_path, output_path, project, plot_type)
where matrix_path, output_path, project, and plot_type must be strings (surrounded by quotation marks, ex: "/Users/ebergstr/Desktop/test_sample").
- The final plots are saved into the user-provided output folder.
AVAILABLE FUNCTIONS
import sigProfilerPlotting as sigPlt
sigPlt.plotSBS(matrix_path, output_path, project, plot_type, percentage=False)
sigPlt.plotDBS(matrix_path, output_path, project, plot_type, percentage=False)
sigPlt.plotID(matrix_path, output_path, project, plot_type, percentage=False)
matrix_path -> path to the mutational matrix of interest
output_path -> desired output path
project -> name of unique sample set
plot_type -> context of the mutational matrix (96, 192, 78, 94, etc.)
percentage -> Boolean: plot the mutational matrix as percentages of the sample's total mutation count. Default is False
To create a sample portrait, ensure that you have a matrix for all required contexts (SBS-6, SBS-24, SBS-96, SBS-384, SBS-1536, DBS-78, DBS-312, ID-83, ID-28, ID-96)
from sigProfilerPlotting import sample_portrait as sP
sP.samplePortrait(sample_matrices_path, output_path, project, percentage=False)
EXAMPLE
This package comes with an example test for each plot type. Run the script plot_example.py from within the downloaded repo after installation:
python3 plot_example.py
This example will create plots for each context for each of the included four samples. These plots will be saved within the BRCA_plot/plots/ folder.
CITATION
Bergstrom EN, Huang MN, Mahto U, Barnes M, Stratton MR, Rozen SG, Alexandrov LB: SigProfilerMatrixGenerator: a tool for visualizing and exploring patterns of small mutational events. BMC Genomics 2019, 20:685 https://bmcgenomics.biomedcentral.com/articles/10.1186/s12864-019-6041-2
COPYRIGHT
Copyright (c) 2020, Erik Bergstrom [Alexandrov Lab] All rights reserved.
Redistribution and use in source and binary forms, with or without modification, are permitted provided that the following conditions are met:
Redistributions of source code must retain the above copyright notice, this list of conditions and the following disclaimer.
Redistributions in binary form must reproduce the above copyright notice, this list of conditions and the following disclaimer in the documentation and/or other materials provided with the distribution.
THIS SOFTWARE IS PROVIDED BY THE COPYRIGHT HOLDERS AND CONTRIBUTORS "AS IS" AND ANY EXPRESS OR IMPLIED WARRANTIES, INCLUDING, BUT NOT LIMITED TO, THE IMPLIED WARRANTIES OF MERCHANTABILITY AND FITNESS FOR A PARTICULAR PURPOSE ARE DISCLAIMED. IN NO EVENT SHALL THE COPYRIGHT HOLDER OR CONTRIBUTORS BE LIABLE FOR ANY DIRECT, INDIRECT, INCIDENTAL, SPECIAL, EXEMPLARY, OR CONSEQUENTIAL DAMAGES (INCLUDING, BUT NOT LIMITED TO, PROCUREMENT OF SUBSTITUTE GOODS OR SERVICES; LOSS OF USE, DATA, OR PROFITS; OR BUSINESS INTERRUPTION) HOWEVER CAUSED AND ON ANY THEORY OF LIABILITY, WHETHER IN CONTRACT, STRICT LIABILITY, OR TORT (INCLUDING NEGLIGENCE OR OTHERWISE) ARISING IN ANY WAY OUT OF THE USE OF THIS SOFTWARE, EVEN IF ADVISED OF THE POSSIBILITY OF SUCH DAMAGE.
CONTACT INFORMATION
Please address any queries or bug reports to Erik Bergstrom at [email protected]