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Update README.md
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tomashhurst authored Jun 21, 2018
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Expand Up @@ -8,6 +8,7 @@ This analysis pipleine uses three main scripts, described below.

### Three main scripts used in the CAPX pipeline


**CAPX-1-preprocess**

Pre-processing of data to prepare it for clustering and dimensionality reduction. Output is a 'clustering ready' .csv file (and .fcs file).
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### Extra scripts for additional functions


**tSNEplots** (https://github.com/sydneycytometry/tSNEplots) can be used to automatically create a coloured tSNE plot for every marker and sample (and group).


**AutoGraph** (https://github.com/sydneycytometry/AutoGraph) can be used to automatically plot dot plots to compare measurements (cells per tissue, median fluorescence intensity (MFI) etc) of each cluster/population between groups.


### The following scripts will be included in the next release of CAPX:


**ClusterPlots** - automated generation of coloured tSNE plots showing clusters.


**FlexiPlots** - a script with adjustable parameters for visualising plots

**SumTables** - generates a table summarising the analysed dataset: samples vs clusters -- number of cells per cluster per sample, MFI of each marker on each cluster in each sample, etc.


**HeatMaps_MFI** - generates a heatmap for measuring MFI for each marker on each cluster, per sample. Includes 'fold-change' visualisation options.


**HeatMaps_CellNum** - generates a heatmap for measuring the number of cells in each cluster in each sample. Includes 'fold-change' visualisation options.


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