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Update README.md
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elileka authored Apr 30, 2024
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Expand Up @@ -156,7 +156,7 @@ In its initial stage, MetaEuk extracts putative coding fragments between stop co

##### The MetaEuk GFF:

In addition to writing a Fasta file, MetaEuk writes a GFF file. Please note that GFF is not perfectly suitable for MetaEuk because MetaEuk doesn't predict non-coding regions. This means that the MetaEuk gene starts and ends where the first and last codons could be matched. The gene and mRNA categories are the same in the MetaEuk GFF. The exon and CDS coordinates will be the same unless a small target overlap was allowed, due to which, the MetaEuk exon was shortened (see above). In this case, the CDS will report the shortening. In the sixth column you can find their individual bitsocres. The contig index starts at 1 and the start coordinate is always smaller than the end coordinate, as required by GFF. The last column contains the **TCS** identifier. Here is an example where a MetaEuk header of two exons is reported in GFF format:
In addition to writing a Fasta file, MetaEuk writes a GFF file. Please note that GFF is not perfectly suitable for MetaEuk because MetaEuk doesn't predict non-coding regions. This means that the MetaEuk gene starts and ends where the first and last codons could be matched. The gene and mRNA categories are the same in the MetaEuk GFF. The exon and CDS coordinates will be the same unless a small target overlap was allowed, due to which, the MetaEuk exon was shortened (see above). In this case, the CDS will report the shortening. In the sixth column you can find their individual bitsocres. The contig index starts at 1 and the start coordinate is always smaller than the end coordinate, as required by GFF. The last column contains the **TCS** identifier, followed by the low_coord of the prediction to support searching for sub-optimal exon sets (see section). Here is an example where a MetaEuk header of two exons is reported in GFF format:

*>protein_acc|contig_acc|-|508|1.15e-150|2|100|911|911[911]:582[582]:330[330]|501[501]:100[100]:402[402]*

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