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charles-plessy committed May 24, 2024
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## Introduction

You need at least two genomes, a _target_, which will be indexed, and one or more _queries_, which will be aligned to the _target_. Paths to the genome files for the _queries_ are passed with the _nf-core_ samplesheet `--input` system, and path to the genome file of the _target_ is passed with the `--target` parameter. Note that the computation is not symmetric: inverting _target_ and _query_ does not lead to strictly identical results.
You need at least two genomes, a _target_, which will be indexed, and one or more _queries_, which will be aligned to the _target_. Paths to the genome files for the _queries_ are passed with the _nf-core_ samplesheet `--input` system, and path to the genome file of the _target_ is passed with the `--target` parameter. Note that the computation is not symmetric: inverting _target_ and _query_ does not lead to strictly identical results.

## Samplesheet input

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- `--m2m`: (default: false) Compute and output the many-to-many alignment.
This adds time and can comsume considerable amount of space; use only
if you need that data.

- By default, `last-split` runs with `-m1e-5` to omit alignments with
mismap probability > 10<sup>−5</sup>, but this can be overriden with
the `--last_split_mismap` option.
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that will be used for the LAST index and that will be prefixed to the
query IDs with a `___` separator.


[`lastal`]: https://gitlab.com/mcfrith/last/-/blob/main/doc/lastal.rst
[`last-dotplot`]: https://gitlab.com/mcfrith/last/-/blob/main/doc/last-dotplot.rst
[LAST seed]: https://gitlab.com/mcfrith/last/-/blob/main/doc/last-seeds.rst
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