Sailfish Beta v0.7.3
This version retains all of the major enhancements introduced since version 0.7.0. In addition, it improves the estimation of each transcript's effective length. With paired-end reads the fragment length distribution will be automatically learned and used to compute the effective length of each transcript. With single-end reads, or when insufficient data is available to estimate the fragment length distribution, the parameter --fldMean
(default value of 200) is used as the mean of the fragment length distribution.
NOTE: Since v0.7.0 the format of the output (the quant.sf
file) written by Sailfish has changed. The RPKM, KPKM and EstNumKmers columns are no longer present. The output format is now equivalent to that of Salmon, and reports, for each record, (1) Transcript Name, (2) Transcript Length, (3) TPM, (4) Estimated Number of Reads. Please be aware of this to make the appropriate changes for tools that perform downstream processing with Sailfish's output.