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QC: Minimal number of control cells #81

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tischi opened this issue May 15, 2020 · 4 comments
Open

QC: Minimal number of control cells #81

tischi opened this issue May 15, 2020 · 4 comments
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@tischi
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tischi commented May 15, 2020 •
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Lets plot histograms of control cell numbers per well and see what is the lowest number we get and than check those wells and the values we get in them to see if something is weird... that should give us a clue of the good value there
@tischi tischi changed the title QC: Minimal number of control cells per image QC: Minimal number of control cells May 15, 2020
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tischi commented May 15, 2020

Number of control cells per well

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tischi commented May 15, 2020

IgG robust z-score vs number of cells per well

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tischi commented May 15, 2020

@metavibor @constantinpape
See above plots for an overview about the number of control cells per well.
I made the lower plot to see whether a too low number of control cells would lead our scores to become unstable. Maybe for plate 413 this could be the case...
Any ideas how to determine a threshold from this? Currently not obvious to me.

@tischi tischi mentioned this issue May 15, 2020
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tischi commented May 15, 2020

By mutual agreement we defined 100 as the threshold.

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