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@@ -6,4 +6,5 @@ phylosignal.Rproj | |
src/*.o | ||
src/*.so | ||
src/*.dll | ||
ignore/ | ||
ignore/ | ||
inst/doc |
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@@ -7,7 +7,7 @@ Author: Francois Keck <[email protected]> | |
Maintainer: Francois Keck <[email protected]> | ||
Description: A collection of tools to explore the phylogenetic signal in univariate and multivariate data. The package provides functions to plot traits data against a phylogenetic tree, different measures and tests for the phylogenetic signal, methods to describe where the signal is located and a phylogenetic clustering method. | ||
License: GPL-3 | ||
Imports: | ||
Imports: | ||
Rcpp (>= 0.11.0), | ||
adephylo, | ||
igraph, | ||
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stats, | ||
utils | ||
LinkingTo: Rcpp, RcppArmadillo | ||
Suggests: testthat | ||
Suggests: testthat, | ||
knitr, | ||
rmarkdown | ||
RoxygenNote: 6.0.1 | ||
LazyLoad: yes | ||
LazyData: true | ||
Encoding: UTF-8 | ||
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VignetteBuilder: knitr |
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--- | ||
title: "Package overview" | ||
author: "Francois Keck" | ||
date: "`r Sys.Date()`" | ||
output: rmarkdown::html_vignette | ||
vignette: > | ||
%\VignetteIndexEntry{Vignette Title} | ||
%\VignetteEngine{knitr::rmarkdown} | ||
%\VignetteEncoding{UTF-8} | ||
--- | ||
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### Data | ||
First, we load the package `phylosignal` and the dataset `carnivora` from `adephylo`. | ||
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```{r, message = FALSE, warning = FALSE} | ||
library(phylosignal) | ||
library(adephylo) | ||
library(ape) | ||
library(phylobase) | ||
data(carni19) | ||
``` | ||
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Here is a phylogenetic tree of 19 carnivora species. | ||
```{r} | ||
tre <- read.tree(text=carni19$tre) | ||
``` | ||
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And we create a dataframe of 3 traits for the 19 carnivora species. | ||
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- Body mass | ||
- Random values | ||
- Simulated values under a Brownian Motion model along the tree | ||
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```{r} | ||
dat <- list() | ||
dat$mass <- carni19$bm | ||
dat$random <- rnorm(19, sd = 10) | ||
dat$bm <- rTraitCont(tre) | ||
dat <- as.data.frame(dat) | ||
``` | ||
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We can combine phylogeny and traits into a `phylo4d` object. | ||
```{r} | ||
p4d <- phylo4d(tre, dat) | ||
``` | ||
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### Visualizing the data | ||
```{r fig.width=8, fig.height=5} | ||
barplot.phylo4d(p4d, tree.type = "phylo", tree.ladderize = TRUE) | ||
``` | ||
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### Measuring and testing the signal for each trait and different methods | ||
```{r} | ||
phyloSignal(p4d = p4d, method = "all") | ||
``` | ||
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### Assessing the behavior of these methods with this phylogeny along a Brownian-Motion influence gradient | ||
```{r message=FALSE, warning=FALSE, results='hide'} | ||
phylosim <- phyloSim(tree = tre, method = "all", nsim = 100, reps = 99) | ||
``` | ||
```{r fig.width=12, fig.height=5} | ||
plot(phylosim, stacked.methods = FALSE, quantiles = c(0.05, 0.95)) | ||
``` | ||
```{r fig.width=5, fig.height=4} | ||
plot.phylosim(phylosim, what = "pval", stacked.methods = TRUE) | ||
``` | ||
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### Assessing the signal depth with correlograms | ||
```{r fig.width=5, fig.height=4} | ||
mass.crlg <- phyloCorrelogram(p4d, trait = "mass") | ||
random.crlg <- phyloCorrelogram(p4d, trait = "random") | ||
bm.crlg <- phyloCorrelogram(p4d, trait = "bm") | ||
plot(mass.crlg) | ||
plot(random.crlg) | ||
plot(bm.crlg) | ||
``` | ||
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### Locating the signal with LIPA | ||
```{r fig.width=6, fig.height=5} | ||
carni.lipa <- lipaMoran(p4d) | ||
carni.lipa.p4d <- lipaMoran(p4d, as.p4d = TRUE) | ||
barplot.phylo4d(p4d, bar.col=(carni.lipa$p.value < 0.05) + 1, center = FALSE , scale = FALSE) | ||
barplot.phylo4d(carni.lipa.p4d, bar.col = (carni.lipa$p.value < 0.05) + 1, center = FALSE, scale = FALSE) | ||
``` |
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--- | ||
title: "Plotting functions" | ||
author: "Francois Keck" | ||
date: "`r Sys.Date()`" | ||
output: rmarkdown::html_vignette | ||
vignette: > | ||
%\VignetteIndexEntry{Vignette Title} | ||
%\VignetteEngine{knitr::rmarkdown} | ||
%\VignetteEncoding{UTF-8} | ||
--- | ||
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The `phylosignal` package comes with functions designed to plot trait values and | ||
phylogeny together. These functions are generics for `phylo4d` objects. This document present | ||
how to use them. | ||
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## Data | ||
First, we load the package `phylosignal` and some others. We will also use the dataset `carnivora` from `adephylo`. | ||
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```{r, message = FALSE, warning = FALSE} | ||
library(ape) | ||
library(adephylo) | ||
library(phylobase) | ||
library(phylosignal) | ||
data(carni19) | ||
``` | ||
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Here is a phylogenetic tree of 19 carnivora species. | ||
```{r} | ||
tre <- read.tree(text = carni19$tre) | ||
``` | ||
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And we create a dataframe of 3 traits for the 19 carnivora species. | ||
- Body mass | ||
- Random values | ||
- Simulated values under a Brownian Motion model along the tree | ||
```{r} | ||
dat <- data.frame(carni19$bm) | ||
dat$random <- rnorm(dim(dat)[1], sd = 10) | ||
dat$bm <- rTraitCont(tre) | ||
``` | ||
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We can combine phylogeny and traits into a `phylo4d` object. | ||
```{r} | ||
p4d <- phylo4d(tre, dat) | ||
``` | ||
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## Basics | ||
Once we have a `phylo4d` object, we can plot it... | ||
There are three plotting functions: `barplot`, `dotplot` and `gridplot`. These functions are actually wrappers of the function `multiplot.phylo4d`. | ||
```{r fig.width=8, fig.height=5} | ||
barplot(p4d) | ||
dotplot(p4d) | ||
gridplot(p4d) | ||
``` | ||
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Each of these functions can be used with one of the 3 tree styles: `phylogram`, `cladogram` and `fan`. | ||
For example, here is a dotplot with a cladogram. | ||
```{r fig.width=8, fig.height=5} | ||
dotplot(p4d, tree.type = "cladogram") | ||
``` | ||
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And here a gridplot with a fan tree. | ||
```{r fig.width=6, fig.height=6} | ||
gridplot(p4d, tree.type = "fan", tip.cex = 0.6, show.trait = FALSE) | ||
``` | ||
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Select the ratio of the plot occupied by the tree. | ||
```{r fig.width=8, fig.height=5} | ||
barplot(p4d, tree.ratio = 0.5) | ||
``` | ||
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Control which traits to plot and their order. | ||
```{r fig.width=8, fig.height=5} | ||
barplot(p4d, trait = c("bm", "carni19.bm")) | ||
``` | ||
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Add simple error bars. | ||
```{r fig.width=8, fig.height=5} | ||
mat.e <- matrix(abs(rnorm(19 * 3, 0, 0.5)), ncol = 3, | ||
dimnames = list(tipLabels(p4d), names(tdata(p4d)))) | ||
barplot(p4d, error.bar.sup = mat.e, error.bar.inf = mat.e) | ||
``` | ||
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It is also possible to open a fan tree with a specified angle. | ||
```{r fig.width=8, fig.height=6} | ||
barplot(p4d, tree.type = "fan", tip.cex = 0.6, tree.open.angle = 160, trait.cex = 0.6) | ||
``` | ||
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## Colors | ||
It's easy to color bars 'by species' with a vector. | ||
```{r fig.width=8, fig.height=5} | ||
barplot(p4d, bar.col = rainbow(19)) | ||
``` | ||
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And for a finer control, one can use a matrix. Here, negative values in red. | ||
```{r fig.width=8, fig.height=5} | ||
mat.col <- ifelse(tdata(p4d, "tip") < 0, "red", "grey35") | ||
barplot(p4d, center = FALSE, bar.col = mat.col) | ||
``` | ||
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Clearly identify traits with colored backgrounds: | ||
```{r fig.width=8, fig.height=5} | ||
barplot(p4d, trait.bg.col = c("#F6CED8", "#CED8F6", "#CEF6CE"), bar.col = "grey35") | ||
``` | ||
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For gridplots, cells are colored with a color palette, using the `cell.col` argument. | ||
```{r fig.width=5, fig.height=6} | ||
gridplot(p4d, tree.type = "fan", tree.ratio = 0.5, | ||
show.trait = FALSE, show.tip = FALSE, | ||
cell.col = terrain.colors(100)) | ||
``` | ||
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Combine arguments for sophisticated plots | ||
```{r fig.width=8, fig.height=5} | ||
tip.col <- rep(1, nTips(p4d)) | ||
tip.col[(mat.col[, 2] == "red") | (mat.col[, 3] == "red")] <- 2 | ||
barplot(p4d, center = FALSE, trait.bg.col = c("#F6CED8", "#CED8F6", "#CEF6CE"), | ||
bar.col = mat.col, tip.col = tip.col, trait.font = c(1, 2, 2)) | ||
``` | ||
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You can control many other things. See `?multiplot.phylo4d` for more informations. | ||
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## Advanced tuning | ||
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In R, it is often possible to add graphical elements to a plot after drawing it (eg. with `points()`, `abline()`, etc.). This is also possible to use such functions with the plots generated with phylosignal. However, as plots are divided in regions (tree, data, tips), you need special functions to interactively browse among them. These functions are `focusTree`, `focusTraits`, `focusTips` and `focusStop`. Let's see how we can use them. | ||
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Add a time scale bar to a tree | ||
```{r fig.width=8, fig.height=5} | ||
barplot(p4d) | ||
focusTree() | ||
add.scale.bar() | ||
``` | ||
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Add a vertical red line to the 2nd trait | ||
```{r fig.width=8, fig.height=5} | ||
barplot(p4d) | ||
focusTraits(2) | ||
abline(v = 1, col = 2) | ||
``` | ||
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Highlight the clade Ursus with a rectangle | ||
```{r fig.width=8, fig.height=5} | ||
barplot(p4d) | ||
focusTips() | ||
rect(xleft = 0, ybottom = 0.5, | ||
xright = 0.95, ytop = 3.5, | ||
col = "#FF000020", border = NA) | ||
``` |