Skip to content
New issue

Have a question about this project? Sign up for a free GitHub account to open an issue and contact its maintainers and the community.

By clicking “Sign up for GitHub”, you agree to our terms of service and privacy statement. We’ll occasionally send you account related emails.

Already on GitHub? Sign in to your account

Run bwtool summary #12

Open
marcelm opened this issue Apr 8, 2020 · 4 comments
Open

Run bwtool summary #12

marcelm opened this issue Apr 8, 2020 · 4 comments

Comments

@marcelm
Copy link
Collaborator

marcelm commented Apr 8, 2020

The shell script that does the scaling/normalization runs bwtool summary. This is not implemented in the Snakemake pipeline, yet.

@marcelm
Copy link
Collaborator Author

marcelm commented Apr 9, 2020

@simonelsasser mentioned that one can also use deepTools, R or Python to extract summary statistics instead.

@marcelm
Copy link
Collaborator Author

marcelm commented Apr 15, 2020

The bwtool summary command is run on a couple of BED files, such as ChromHMM17.bed and K27_genes_bwtool.bed. If we integrate this or a similar tool into the pipeline, we would need a way to configure which BED files to run it on. Ideas:

  • List the paths to the BED files in the configuration file
  • Use all BED files that exist in a subdirectory; then the user would have to create symlinks in it

@cnluzon
Copy link
Collaborator

cnluzon commented Apr 15, 2020

I'm more inclined to use the BED paths in the config, even though it can be a bit tiresome to specify each one, I think it pays off in flexibility. And if it turns out the BED files we use don't usually change, configure once and run many times.

@marcelm
Copy link
Collaborator Author

marcelm commented Apr 15, 2020

Yes, good point. This also brings up the point of how to run the pipeline on many datasets easily, need to think about that.

Sign up for free to join this conversation on GitHub. Already have an account? Sign in to comment
Labels
None yet
Projects
None yet
Development

No branches or pull requests

2 participants