Before you set off your Oxford Nanopore sequencing run, make sure to pass along the following information to your friendly neighborhood bioinformatician:
- Experiment ID: An identifier or number for this run.
- Instrument name: Most instruments have an IP address as well as a name or alias, either of which can be used to access them remotely.
- IP Address: The address on the building network where raw data from the run will be stored.
- Data File Path: The absolute file path, starting from the root (
/), where raw signal data will be placed by the instrument. - Barcode Kit: The Nanopore kit used to barcode fragments from each sample prior to multiplexing. These kits' names generally look something like "SQK-NBD114-24" or "SQK-RBK114-24".
- Flow Cell Chemistry: While not required for basecalling, the flow cell version used can help set expectations for run QC. The chemistry will look something like "R10.4.1".
- Number of Barcodes Used: Also not necessary for basecalling, but still helpful for tracking how many barcodes should have non-negligible numbers of reads.
Additionally, you might also want to pass on a sample sheet in the format:
barcode,sample_id
1,sample_1
2,sample_2
This information can be used during basecalling or demultiplexing to give a more useful name to the output files.
For you convenience, and Excel sheet with a Run Metadata sheet and a Samplesheet sheet is included in this repo.