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Method to run a BLASTp, and from the HSP protein accessions, retrieve the GBK record from its' genome.

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cross12tamu/blast2gbk

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BLASTp --> GBK record

Summary:

Method(s) that from a BLASTp, and from the HSP protein accessions, retrieve the GBK record for each accession. Additionally, a FASTA file containing all HSPs can be output if desired.

Requirements:

  • BioPython Modules
    • Blast
    • Entrez
    • SeqIO

Script:

Several Parts:

  1. Fetch FASTA file based on desired protein for BLASTp

  2. Run BLASTp with fetched FASTA file

  3. Parse BLASTp Results

  4. IDentify "best" method from GBK file retrieval

    a) esearch

    b) elink

    c) efetch

  5. FASTA export (if desired)

Jupyter Notebook:

  • Mostly the same as the retrieveGBK.py script; but contains more notes and description than the script.

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Method to run a BLASTp, and from the HSP protein accessions, retrieve the GBK record from its' genome.

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