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Wastewater sample processing

This workflow processes wastewater samples for the detection of drug resistance mutations.

Input:

  • aligned samples (alignment with V-pipe)
  • positions of interest: positions where drug resistance mutations are expected
  • list of all samples to be processed

Output:

  • csv file of all mutations at positions of interest --> This file can be used in the visualisation notebook to create a heatmap

To run this workflow adapt file paths in config and sample names. Run the pipeline on Euler with run_workflow.sh.

Note, at the moment the workflow is SARS-CoV-2 specific, however, reference files and gene annotations can be easily swapped.