MiXCR is a universal software for fast and accurate analysis of raw T- or B- cell receptor repertoire sequencing data.
brew install milaboratory/all/mixcr
to upgrade already installed MiXCR to the newest version:
brew update
brew upgrade mixcr
- download latest MiXCR version from release page
- unzip the archive
- add resulting folder to your
PATHvariable- or add symbolic link for
mixcrscript to yourbinfolder - or use MiXCR directly by specifying full path to the executable script
- or add symbolic link for
- Any OS with Java support (Linux, Windows, Mac OS X, etc..)
- Java 1.8 or higher
Here is a very simple example of analysis of raw human RepSeq data:
mixcr align -r log.txt input_R1.fastq.gz input_R2.fastq.gz alignments.vdjca
mixcr assemble -r log.txt alignments.vdjca clones.clns
mixcr exportClones clones.clns clones.txt
this sequence of commands will produce a tab-delimited list of clones (clones.txt) assembled by their CDR3 sequences with extensive information on their abundancies, V, D and J genes etc.
For more details see documentation.
Detailed documentation can be found at https://mixcr.readthedocs.io/
Copyright (c) 2014-2015, Bolotin Dmitry, Chudakov Dmitry, Shugay Mikhail (here and after addressed as Inventors) All Rights Reserved
Permission to use, copy, modify and distribute any part of this program for educational, research and non-profit purposes, by non-profit institutions only, without fee, and without a written agreement is hereby granted, provided that the above copyright notice, this paragraph and the following three paragraphs appear in all copies.
Those desiring to incorporate this work into commercial products or use for commercial purposes should contact the Inventors using one of the following email addresses: [email protected], [email protected]
IN NO EVENT SHALL THE INVENTORS BE LIABLE TO ANY PARTY FOR DIRECT, INDIRECT, SPECIAL, INCIDENTAL, OR CONSEQUENTIAL DAMAGES, INCLUDING LOST PROFITS, ARISING OUT OF THE USE OF THIS SOFTWARE, EVEN IF THE INVENTORS HAS BEEN ADVISED OF THE POSSIBILITY OF SUCH DAMAGE.
THE SOFTWARE PROVIDED HEREIN IS ON AN "AS IS" BASIS, AND THE INVENTORS HAS NO OBLIGATION TO PROVIDE MAINTENANCE, SUPPORT, UPDATES, ENHANCEMENTS, OR MODIFICATIONS. THE INVENTORS MAKES NO REPRESENTATIONS AND EXTENDS NO WARRANTIES OF ANY KIND, EITHER IMPLIED OR EXPRESS, INCLUDING, BUT NOT LIMITED TO, THE IMPLIED WARRANTIES OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, OR THAT THE USE OF THE SOFTWARE WILL NOT INFRINGE ANY PATENT, TRADEMARK OR OTHER RIGHTS.
Bolotin, Dmitriy A., Stanislav Poslavsky, Igor Mitrophanov, Mikhail Shugay, Ilgar Z. Mamedov, Ekaterina V. Putintseva, and Dmitriy M. Chudakov. "MiXCR: software for comprehensive adaptive immunity profiling." Nature methods 12, no. 5 (2015): 380-381.
Can be found here.