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@@ -27,3 +27,6 @@ SOPs serve as valuable educational resources for training new researchers and st | |
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The listed experimental SOPs that can be found on this Knowledge Base have been collected by NFDI4Microbiota's consortia members and participants from either (1) well-established and published sources or (2) consist of well-running in-house protocols that have been recently established by various research consortia and their domain expert with wet-lab experiences. | ||
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# Get Help | ||
If you have any further questions about the management and analysis of your microbial research data, please contact us: [[email protected]](mailto:[email protected]) (by emailing us you agree to the privacy policy on our website: [Contact](https://nfdi4microbiota.de/contact-form/)) |
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@@ -22,3 +22,6 @@ This page lists well-established and tested protocols from the International Hum | |
|18S Illumina Amplicon Protocol| Earth Microbiome | DNA Sequencing | [16S Illumina Amplicon Protocol](https://www.protocols.io/view/emp-18s-illumina-amplicon-protocol-ewov1b6pgr24/v2) | | ||
|ITS Illumina Amplicon Protocol| Earth Microbiome | DNA Sequencing | [ITS Illumina Amplicon Protocol](https://www.protocols.io/view/emp-its-illumina-amplicon-protocol-14egnqypg5dy/v1) | | ||
{: .table .table-hover} | ||
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# Get Help | ||
If you have any further questions about the management and analysis of your microbial research data, please contact us: [[email protected]](mailto:[email protected]) (by emailing us you agree to the privacy policy on our website: [Contact](https://nfdi4microbiota.de/contact-form/)) |
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# Source | ||
In-house protocol provided by Sarah Schulz | ||
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# Get Help | ||
If you have any further questions about the management and analysis of your microbial research data, please contact us: [[email protected]](mailto:[email protected]) (by emailing us you agree to the privacy policy on our website: [Contact](https://nfdi4microbiota.de/contact-form/)) |
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- Bile samples - ZymoBIOMICS DNA/RNA Miniprep Kit - in house protocol provided by Nicole Treichel | ||
- mouse small intestine content - ZymoBIOMICS DNA/RNA Miniprep Kit - in house protocol provided by Nicole Treichel | ||
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# Get Help | ||
If you have any further questions about the management and analysis of your microbial research data, please contact us: [[email protected]](mailto:[email protected]) (by emailing us you agree to the privacy policy on our website: [Contact](https://nfdi4microbiota.de/contact-form/)) | ||
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## References | ||
{% bibliography --cited_in_order %} |
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@@ -47,6 +47,9 @@ Specific instructions for homogenization of frozen tissues (eg; liver) prior to | |
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*It is difficult to process tissue samples at weights below 10 mg, so aim for at least 10 mg of tissue to start with | ||
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# Get Help | ||
If you have any further questions about the management and analysis of your microbial research data, please contact us: [[email protected]](mailto:[email protected]) (by emailing us you agree to the privacy policy on our website: [Contact](https://nfdi4microbiota.de/contact-form/)) | ||
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## References | ||
{% bibliography --cited_in_order %} | ||
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17. Submit dried sample in 1.5 ml eppendorf tube and can be stored at in dried ice. | ||
18. Blank control: prepare processed blank sample using same procedure but without biological sample (use water or buffer instead). | ||
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# Get Help | ||
If you have any further questions about the management and analysis of your microbial research data, please contact us: [[email protected]](mailto:[email protected]) (by emailing us you agree to the privacy policy on our website: [Contact](https://nfdi4microbiota.de/contact-form/)) | ||
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## References | ||
{% bibliography --cited_in_order %} |
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7. Sample are reconstituted in mobile phase (or 50% methanol) before analysis | ||
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# Get Help | ||
If you have any further questions about the management and analysis of your microbial research data, please contact us: [[email protected]](mailto:[email protected]) (by emailing us you agree to the privacy policy on our website: [Contact](https://nfdi4microbiota.de/contact-form/)) | ||
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## References | ||
{% bibliography --cited_in_order %} |
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10. Elute with 50 µl B | ||
Dry samples in Speedvac and reconstitute peptides in 1% formic acid supplemented with 4% acetonitrile. Samples are now ready for the injection on a mass spectrometer | ||
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# Get Help | ||
If you have any further questions about the management and analysis of your microbial research data, please contact us: [[email protected]](mailto:[email protected]) (by emailing us you agree to the privacy policy on our website: [Contact](https://nfdi4microbiota.de/contact-form/)) | ||
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## References | ||
{% bibliography --cited_in_order %} | ||
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| Yoghurt | after purchase, samples were placed immediately at 4°C for transport and within 12 h stared at -80°C | {% cite Islam_2021 %} | | ||
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# Get Help | ||
If you have any further questions about the management and analysis of your microbial research data, please contact us: [[email protected]](mailto:[email protected]) (by emailing us you agree to the privacy policy on our website: [Contact](https://nfdi4microbiota.de/contact-form/)) | ||
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## References | ||
{% bibliography --cited_in_order %} |
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</div> --> | ||
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## NFDI4Microbiota - Democratize access to microbiota data and high-end computational analyses | ||
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NFDI4Microbiota is a consortium that is part of the German NFDI (National research Data Infrastructure), aimed at advancing microbiological research through digital transformation. | ||
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### Mission | ||
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Our mission is to be the central hub in Germany for supporting the microbiology community with access to (meta)data, analysis services, data/metadata standards, training and infrastructure. Given microbiology's relevance to medicine, agriculture, biotechnology, and earth sciences, the consortium's work strives for a broader societal impact. | ||
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### Vision | ||
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The vision of the NFDI4Microbiota consortium is that researchers in microbiology (including bacteriology, virology, protistology, mycology and parasitology) can translate research data easily into a deep understanding of microbial species and their interactions on a molecular level. | ||
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### What we aim and strive for | ||
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We aim to foster a cultural shift towards open science and reproducible research by promoting the FAIR principles, that are, Findable, Accessible, Interoperable, and Reusable research. We offer training to enhance researchers' skills and data literacy, thereby improving the overall quality of research. By integrating microbial expertise, we aim to augment the value of other NFDI consortia and strengthen connections within the national and international microbiology communities. | ||
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We strive to improve research processes by organizing and linking microbial (meta)data, supporting high-quality (meta)data management through data stewards, and enhancing (meta)data reproducibility through standardized processing and analysis. Additionally, we are developing computational tools to translate (meta)data into new insights and creating a central information hub tailored to the needs of the microbial research community. | ||
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### Where to find us | ||
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More information on how to contribute to the consortium, participate in coffee talks, become an ambasador or take part in training events, visit the [NFDI4Microbiota webpage](https://nfdi4microbiota.de/). If you have questions feel free to contact us via the [Helpdesk](https://nfdi4microbiota.de/contact-form/) or contact us directly via [email](mailto:[email protected]). | ||
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By sending an email you agree with the NFDI4Microbiota Consortium Privacy Policy, found bellow the Helpdesk contact form. | ||
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## What is this Knowledge Base about? | ||
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This knowledge base is a collection of information and references with | ||
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@@ -345,3 +345,6 @@ learned/been exposed to during the event. You are also encouraged to | |
benefit from the work you already provided. If you do so, make sure to | ||
state under what license you provide your work. We suggest a permissive | ||
license such as [CC BY](https://creativecommons.org/licenses/by/4.0/), or „CC 0“. | ||
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# Get Help | ||
If you have any further questions about the management and analysis of your microbial research data, please contact us: [[email protected]](mailto:[email protected]) (by emailing us you agree to the privacy policy on our website: [Contact](https://nfdi4microbiota.de/contact-form/)) |
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