This work is published at http://dx.doi.org/10.1039/D3LC00017F
Details of model please refer to Supplmentary doc: https://www.rsc.org/suppdata/d3/lc/d3lc00017f/d3lc00017f1.pdf
Confusion matrices obtained after subselection of spectral windows for the validation set using the PCA-LSTM model.
Method overview:
Authors: Huang, Steven H. and Sartorello, Giovanni and Shen, Po-Ting and Xu, Chengqi and Elemento, Olivier and Shvets, Gennady
Title: "Metasurface-enhanced infrared spectroscopy in multiwell format for real-time assaying of live cells"
Abstract: Fourier transform infrared (FTIR) spectroscopy is a popular technique for the analysis of biological samples, yet its application in characterizing live cells is limited due to the strong attenuation of mid-IR light in water. Special thin flow cells and attenuated total reflection (ATR) FTIR spectroscopy have been used to mitigate this problem, but these techniques are difficult to integrate into a standard cell culture workflow. In this work, we demonstrate that the use of a plasmonic metasurface fabricated on planar substrates and the probing of cellular IR spectra through metasurface-enhanced infrared spectroscopy (MEIRS) can be an effective technique to characterize the IR spectra of live cells in a high-throughput manner. Cells are cultured on metasurfaces integrated with multiwell cell culture chambers and are probed from the bottom using an inverted FTIR micro-spectrometer. To demonstrate the use of MEIRS as a cellular assay, cellular adhesion on metasurfaces with different surface coatings and cellular response to the activation of the protease-activated receptor (PAR) signaling pathway were characterized through the changes in cellular IR spectra.