LSM3241 Searching for transposons inserted into the yeast genome with NGS data

A transposon, Ty5-6p of family TY5 was inserted into the genome of yeast strain S288C. Here, equipped with simulated NGS whole-genome sequencing data, I show the use of commandline tools and simulation techniques to find the positions, orientation and functional consequence of the transposon inserts in the genome of yeast strain S288C.

Workflow

Contents

1. A full written report is provided in /Report as PDF.
2. Two scripts are provided: [A] NGS Transposon Bash and Python Script, for commandline and python simulation codes and [B] Gviz script.R, for locating the genes in which the transposon is inserted.