revert positive=FALSE

AlexsLemonade · May 29, 2024 · aa73641 · aa73641
1 parent d2bd341
commit aa73641
Showing 1 changed file with 1 addition and 1 deletion.
diff --git a/scRNA-seq/04-dimension_reduction_scRNA.Rmd b/scRNA-seq/04-dimension_reduction_scRNA.Rmd
@@ -253,7 +253,7 @@ filtered_sce <- filtered_sce[, filtered_sce$sum >= 500]
 
 qclust <- scran::quickCluster(filtered_sce)
 
-filtered_sce <- scran::computeSumFactors(filtered_sce, clusters = qclust, positive = FALSE)
+filtered_sce <- scran::computeSumFactors(filtered_sce, clusters = qclust)
 ```
 
 Looks good! Now we'll do the normalization.