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Merge pull request #258 from AlexsLemonade/sjspielman/254-adt-rowdata…
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Update adt altExp rowData docs
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sjspielman authored Jan 31, 2024
2 parents 1227fe9 + 7d80fae commit 3afe4ae
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3 changes: 2 additions & 1 deletion docs/merged_objects.md
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Expand Up @@ -77,7 +77,7 @@ The following per-cell data columns are included for each cell, calculated using
| `submitter_celltype_annotation` | If available, cell type annotations obtained from the group that submitted the original data. Cells that the submitter did not annotate are labeled as `"Submitter-excluded"` |
| `singler_celltype_annotation` | If cell typing with `SingleR` was performed, the annotated cell type. Cells labeled as `NA` are those which `SingleR` could not confidently annotate. If cell typing was performed for some but not all libraries in the merged object, libraries without annotations will be labeled `"Cell type annotation not performed"` |
| `singler_celltype_ontology` | If cell typing with `SingleR` was performed with ontology labels, the annotated cell type's ontology ID. Cells labeled as `NA` are those which `SingleR` could not confidently annotate. If cell typing was performed for some but not all libraries in the merged object, libraries without annotations will be labeled `"Cell type annotation not performed"` |
| `cellassign_celltype_annotation` | If cell typing with `CellAssign` was performed, the annotated cell type. Cells labeled as `"other"` are those which `CellAssign` could not confidently annotate. If cell typing was performed for some but not all libraries in the merged object, libraries without annotations will be labeled `"Cell type annotation not performed"`. If `CellAssign` was unable to complete successfully for a given library, cells will be labeled as `Not run` |
| `cellassign_celltype_annotation` | If cell typing with `CellAssign` was performed, the annotated cell type. Cells labeled as `"other"` are those which `CellAssign` could not confidently annotate. If cell typing was performed for some but not all libraries in the merged object, libraries without annotations will be labeled `"Cell type annotation not performed"`. If `CellAssign` was unable to complete successfully for a given library, cells will be labeled as `"Not run"` |
| `cellassign_max_prediction` | If cell typing with `CellAssign` was performed and completed successfully, the annotation's prediction score (probability) |


Expand Down Expand Up @@ -295,6 +295,7 @@ The columns `mean-SCPCL000000` and `detected-SCPCL000000` are present for each l

| Column name | Contents |
| ----------- | -------------------------------------------------------------- |
| `adt_id` | Name or ID of the ADT |
| `mean-SCPCL000000` | Mean ADT count across all cells/droplets. Only present for libraries with CITE-seq data |
| `detected-SCPCL000000` | Percent of cells in which the ADT was detected (ADT count > 0 ). Only present for libraries with CITE-seq data |
| `target_type` | Whether each ADT is a target (`target`), negative/isotype control (`neg_control`), or positive control (`pos_control`). If this information was not provided, all ADTs will have been considered targets and will be labeled as `target` |
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3 changes: 2 additions & 1 deletion docs/sce_file_contents.md
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Expand Up @@ -89,7 +89,7 @@ Further, if cell type annotation was performed, there will be additional columns
| `cluster` | Cell cluster identity identified by graph-based clustering |
| `singler_celltype_annotation` | If cell typing with `SingleR` was performed, the annotated cell type. Cells labeled as `NA` are those which `SingleR` could not confidently annotate |
| `singler_celltype_ontology` | If cell typing with `SingleR` was performed with ontology labels, the annotated cell type's ontology ID. Cells labeled as `NA` are those which `SingleR` could not confidently annotate |
| `cellassign_celltype_annotation` | If cell typing with `CellAssign` was performed, the annotated cell type. Cells labeled as `"other"` are those which `CellAssign` could not confidently annotate. If `CellAssign` was unable to complete successfully, cells will be labeled as `Not run` |
| `cellassign_celltype_annotation` | If cell typing with `CellAssign` was performed, the annotated cell type. Cells labeled as `"other"` are those which `CellAssign` could not confidently annotate. If `CellAssign` was unable to complete successfully, cells will be labeled as `"Not run"` |
| `cellassign_max_prediction` | If cell typing with `CellAssign` was performed and completed successfully, the annotation's prediction score (probability) |

### SingleCellExperiment gene information and metrics
Expand Down Expand Up @@ -260,6 +260,7 @@ This data frame contains the following columns with statistics for each ADT:

| Column name | Contents |
| ----------- | -------------------------------------------------------------- |
| `adt_name` | Name or ID of the ADT |
| `mean` | Mean ADT count across all cells/droplets |
| `detected` | Percent of cells in which the ADT was detected (ADT count > 0 ) |
| `target_type` | Whether each ADT is a target (`target`), negative/isotype control (`neg_control`), or positive control (`pos_control`). If this information was not provided, all ADTs will have been considered targets and will be labeled as `target` |
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