MS-DIAL Data Processing Issue: Direct Infusion Analysis on SCIEX QTRAP API 4000

[Mudjgiwa]

System and Software Details

• Instrument: SCIEX QTRAP API 4000
• Acquisition Method: Direct Infusion with Multi Precursor Ion Scan
• Software: MS-DIAL version 5.4
• Analysis Type: Lipidomics

Current Situation

• I'm attempting to process direct infusion data from Multi Precursor Ion Scan experiments but encountering consistent software crashes in MS-DIAL.

• Issue persists even after separating files into positive and negative ionization modes

• The same MS-DIAL installation successfully processes LC-MS DDA proteomics data

Troubleshooting Steps Taken

1. Initially attempted to process inexplicitly unseparated raw files into Positive and Negative ions
2. Separated lipidomics data into positive and negative mode files
3. Tested software functionality with other data types (LC-MS DDA)

Questions

1. Is MS-DIAL compatible with direct infusion data from Multi Precursor Ion Scan experiments?
2. Could the issue be related to data acquisition parameters rather than software compatibility?
3. Are there specific requirements for processing direct infusion data in MS-DIAL?

Specific Requirements

1. Need guidance on whether direct infusion data is supported
2. If supported, what are the correct parameters for processing such data?
3. Are there any known limitations or special considerations for QTRAP API 4000 raw data?