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limma:MakeContrasts integration issues #309

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aodainic7 opened this issue Feb 6, 2024 · 2 comments · Fixed by #311
Closed

limma:MakeContrasts integration issues #309

aodainic7 opened this issue Feb 6, 2024 · 2 comments · Fixed by #311
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@aodainic7
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Hello Stefano,
in the pipeline you can use contrasts for the test_differential_abundance() to extract the comparison you are interested in. When using a formula with interaction (e.g. treatment*genotype), you create columns with colon (treatment:genotype) and limma::MakeContrasts does not accept it. If you manually modify the design matrix by exchanging : with _ the function works.
Cheers,
Alex

@stemangiola stemangiola linked a pull request Feb 7, 2024 that will close this issue
@stemangiola stemangiola self-assigned this Feb 7, 2024
@stemangiola stemangiola added the bug Something isn't working label Feb 7, 2024
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@aodainic7 test the branch #311

@aodainic7
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fixed

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