diff --git a/.github/workflows/build-numpy-2.yml b/.github/workflows/build-numpy-2.yml
index 013ecec7e..4e1c14890 100644
--- a/.github/workflows/build-numpy-2.yml
+++ b/.github/workflows/build-numpy-2.yml
@@ -23,6 +23,8 @@ jobs:
       run: |
         python -m pip install --upgrade pip
         pip install -r requirements-numpy2.txt -r requirements-numpy2-dev.txt
+        # update bio2zarr for NumPy 2, see https://github.com/sgkit-dev/bio2zarr/issues/256
+        pip install -U 'numpy<2.1' -U git+https://github.com/sgkit-dev/bio2zarr.git
     # - name: Run pre-commit
     #   uses: pre-commit/action@v2.0.0
     - name: Test with pytest (numba jit disabled)
diff --git a/requirements-numpy2-dev.txt b/requirements-numpy2-dev.txt
index f2370bd5e..275c7d538 100644
--- a/requirements-numpy2-dev.txt
+++ b/requirements-numpy2-dev.txt
@@ -14,7 +14,6 @@ bed-reader
 rechunker
 cbgen > 1.0.5; platform_system != "Windows"
 cbgen == 1.0.1; platform_system == "Windows"
-bio2zarr @ git+https://github.com/sgkit-dev/bio2zarr.git; platform_system != "Windows"
 yarl
 matplotlib
 asv