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stainedglass self-alignment heatmap #14

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amakunin opened this issue Nov 28, 2023 · 1 comment
Open

stainedglass self-alignment heatmap #14

amakunin opened this issue Nov 28, 2023 · 1 comment
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@amakunin
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amakunin commented Nov 28, 2023

Description of feature

stainedglass is particularly useful for disentangling tandem repeat structures and segmental duplications. Under the hood, it is a snakemake workflow of bowtie2 alignments of chunked genome + some custom scripts for transformation of the alignments into a heatmap. It can produce higlass-compatible cooler output:

cd StainedGlass
samtools faidx ref.fa
snakemake --use-conda --cores 16 cooler_density --config nbatch=4 alnthreads=4 fasta=ref.fa window=32 cooler_window=100 sample=sample_id
@amakunin amakunin added the enhancement Improvement of the existing features label Nov 28, 2023
@muffato muffato added feature Requests for new features user request Requests made by users and public and removed enhancement Improvement of the existing features labels Jun 1, 2024
@muffato muffato added the duplicate This issue or pull request already exists label Jun 26, 2024
@muffato
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muffato commented Jun 26, 2024

We should favour ModDotPlot which is a faster implementation, though it doesn't yet have mcool support.

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