From bd6e979133f977bfb35ff04c90d7a0125f7be929 Mon Sep 17 00:00:00 2001 From: Takuto Sato Date: Tue, 29 Aug 2023 13:44:51 -0400 Subject: [PATCH] ready --- .../java/htsjdk/samtools/BAMFileReader.java | 1 + .../java/htsjdk/samtools/BamFileIoUtils.java | 66 +++++++----------- src/main/java/htsjdk/samtools/SAMUtils.java | 4 +- .../htsjdk/samtools/SamFileValidator.java | 4 +- .../util/BlockCompressedInputStream.java | 17 ++++- .../samtools/BamFileIoUtilsUnitTest.java | 44 ++++-------- .../java/htsjdk/samtools/HtsjdkTestUtils.java | 3 +- ...rio.HiSeq.WGS.b37.NA12878.20.first.500.bai | Bin 0 -> 96 bytes ...rio.HiSeq.WGS.b37.NA12878.20.first.500.bam | Bin 0 -> 130040 bytes 9 files changed, 63 insertions(+), 76 deletions(-) create mode 100644 src/test/resources/htsjdk/samtools/cram/CEUTrio.HiSeq.WGS.b37.NA12878.20.first.500.bai create mode 100644 src/test/resources/htsjdk/samtools/cram/CEUTrio.HiSeq.WGS.b37.NA12878.20.first.500.bam diff --git a/src/main/java/htsjdk/samtools/BAMFileReader.java b/src/main/java/htsjdk/samtools/BAMFileReader.java index 0addba3773..c3ee23f04d 100644 --- a/src/main/java/htsjdk/samtools/BAMFileReader.java +++ b/src/main/java/htsjdk/samtools/BAMFileReader.java @@ -32,6 +32,7 @@ import java.io.File; import java.io.IOException; import java.io.InputStream; +import java.nio.file.Path; import java.util.ArrayList; import java.util.Arrays; import java.util.List; diff --git a/src/main/java/htsjdk/samtools/BamFileIoUtils.java b/src/main/java/htsjdk/samtools/BamFileIoUtils.java index 9db977b06e..3dd45f463f 100644 --- a/src/main/java/htsjdk/samtools/BamFileIoUtils.java +++ b/src/main/java/htsjdk/samtools/BamFileIoUtils.java @@ -1,5 +1,6 @@ package htsjdk.samtools; +import htsjdk.beta.exception.HtsjdkException; import htsjdk.samtools.cram.io.CountingInputStream; import htsjdk.samtools.seekablestream.SeekablePathStream; import htsjdk.samtools.util.BlockCompressedFilePointerUtil; @@ -85,67 +86,54 @@ public static void blockCopyBamFile(final File inputFile, final OutputStream out } /** - * Copy data from a BAM file to an OutputStream by directly copying the gzip blocks + * Copy data from a BAM file to an OutputStream by directly copying the gzip blocks. * - * @param inputFile The file to be copied + * @param inputFile The BAM file to be copied * @param outputStream The stream to write the copied data to * @param skipHeader If true, the header of the input file will not be copied to the output stream * @param skipTerminator If true, the terminator block of the input file will not be written to the output stream */ public static void blockCopyBamFile(final Path inputFile, final OutputStream outputStream, final boolean skipHeader, final boolean skipTerminator) { - // tsato: use CountingInputStream to replace FileInputStream. The latter has .getChannel().getPosition - // The regular InputStream from Files.newInputStream() doesn't have it, but I might be able to do so by creating Channel object and avoid using CountingInputStream - try (final CountingInputStream in = new CountingInputStream(Files.newInputStream(inputFile)); - final SeekablePathStream in2 = new SeekablePathStream(inputFile)){ - // try (final InputStream in = Files.newInputStream(inputFile)){ + try (final SeekablePathStream in = new SeekablePathStream(inputFile)){ // a) It's good to check that the end of the file is valid and b) we need to know if there's a terminator block and not copy it if skipTerminator is true final BlockCompressedInputStream.FileTermination term = BlockCompressedInputStream.checkTermination(inputFile); if (term == BlockCompressedInputStream.FileTermination.DEFECTIVE) throw new SAMException(inputFile.toUri() + " does not have a valid GZIP block at the end of the file."); - if (skipHeader) { // tsato: this method assumes bam file...should I test cram? - // tsato: reason virtual offset of first read when the input is file doesn't need seekable stream is the blockcompressed one - // calls seekable. Then - final long vOffsetOfFirstRecord0 = SAMUtils.findVirtualOffsetOfFirstRecordInBam(inputFile.toFile()); // tsato: just for testing purposes + if (skipHeader) { final long vOffsetOfFirstRecord = SAMUtils.findVirtualOffsetOfFirstRecordInBam(inputFile); - int d = 3; - - final SeekablePathStream seekablePathStream = new SeekablePathStream(inputFile); - final BlockCompressedInputStream blockIn = new BlockCompressedInputStream(seekablePathStream); // Louis: Have BlockCompressedInputStream take a path? - blockIn.seek(vOffsetOfFirstRecord); // This is why we give the BlockCompressedInputStream a SeekablePathStream rather than the regular InputStream - final long remainingInBlock = blockIn.available(); // Louis is probably concerned that I'm passing the same seekablePathStream object - - // If we found the end of the header then write the remainder of this block out as a - // new gzip block and then break out of the while loop - if (remainingInBlock >= 0) { - final BlockCompressedOutputStream blockOut = new BlockCompressedOutputStream(outputStream, (Path)null); - IOUtil.transferByStream(blockIn, blockOut, remainingInBlock); - blockOut.flush(); - // Don't close blockOut because closing underlying stream would break everything - } - - long pos = BlockCompressedFilePointerUtil.getBlockAddress(blockIn.getFilePointer()); // tsato: what exactly is a filepointer... - blockIn.close(); - in2.seek(pos); // per Louis. But CountingInputStream cannot seek. - while (pos > 0) { - pos -= in.skip(pos); + // tsato: curious --- why do we need BlockCompressedInputStream at all here? + try (final BlockCompressedInputStream blockIn = new BlockCompressedInputStream(inputFile)) { + blockIn.seek(vOffsetOfFirstRecord); + final long remainingInBlock = blockIn.available(); + + // If we found the end of the header then write the remainder of this block out as a + // new gzip block and then break out of the while loop + if (remainingInBlock >= 0) { + final BlockCompressedOutputStream blockOut = new BlockCompressedOutputStream(outputStream, (Path) null); + IOUtil.transferByStream(blockIn, blockOut, remainingInBlock); + blockOut.flush(); + // Don't close blockOut because closing underlying stream would break everything + } + + final long pos = BlockCompressedFilePointerUtil.getBlockAddress(blockIn.getFilePointer()); + blockIn.close(); + + in.seek(pos); + } catch (IOException e){ + throw new HtsjdkException("Encountered an error ", e); } } // Copy remainder of input stream into output stream - final long currentPos = in.getCount(); // tsato: assuming currentPos is the position after writing the first block, this should be ok. - final long currentPos2 = in2.position(); - if (currentPos2 != currentPos){ - int d = 3; - } + final long currentPos = in.position(); final long length = Files.size(inputFile); final long skipLast = ((term == BlockCompressedInputStream.FileTermination.HAS_TERMINATOR_BLOCK) && skipTerminator) ? BlockCompressedStreamConstants.EMPTY_GZIP_BLOCK.length : 0; final long bytesToWrite = length - skipLast - currentPos; - // tsato: test in2 - IOUtil.transferByStream(in2, outputStream, bytesToWrite); + IOUtil.transferByStream(in, outputStream, bytesToWrite); } catch (final IOException ioe) { throw new RuntimeIOException(ioe); } diff --git a/src/main/java/htsjdk/samtools/SAMUtils.java b/src/main/java/htsjdk/samtools/SAMUtils.java index 31d92cdf5c..fde94cde8c 100644 --- a/src/main/java/htsjdk/samtools/SAMUtils.java +++ b/src/main/java/htsjdk/samtools/SAMUtils.java @@ -679,9 +679,9 @@ public static int combineMapqs(int m1, int m2) { } + public static long findVirtualOffsetOfFirstRecordInBam(final Path bamFile) { - try { - SeekableStream ss = new SeekablePathStream(bamFile); + try (SeekableStream ss = new SeekablePathStream(bamFile)){ return BAMFileReader.findVirtualOffsetOfFirstRecord(ss); } catch (final IOException ioe) { throw new RuntimeEOFException(ioe); diff --git a/src/main/java/htsjdk/samtools/SamFileValidator.java b/src/main/java/htsjdk/samtools/SamFileValidator.java index 50f289e3ab..8f4edf36a1 100644 --- a/src/main/java/htsjdk/samtools/SamFileValidator.java +++ b/src/main/java/htsjdk/samtools/SamFileValidator.java @@ -210,7 +210,7 @@ public boolean validateSamFileVerbose(final SamReader samReader, final Reference } public void validateBamFileTermination(final File inputFile) { - validateBamFileTermination(inputFile.toPath()); + validateBamFileTermination(IOUtil.toPath(inputFile)); } public void validateBamFileTermination(final Path inputFile) { @@ -222,7 +222,7 @@ public void validateBamFileTermination(final Path inputFile) { BlockCompressedInputStream.checkTermination(inputFile); if (terminationState.equals(BlockCompressedInputStream.FileTermination.DEFECTIVE)) { addError(new SAMValidationError(Type.TRUNCATED_FILE, "BAM file has defective last gzip block", - inputFile.toAbsolutePath().toString())); // tsato: confirm toAbsolutePath() is the right thing to do here + inputFile.toUri().toString())); } else if (terminationState.equals(BlockCompressedInputStream.FileTermination.HAS_HEALTHY_LAST_BLOCK)) { addError(new SAMValidationError(Type.BAM_FILE_MISSING_TERMINATOR_BLOCK, "Older BAM file -- does not have terminator block", diff --git a/src/main/java/htsjdk/samtools/util/BlockCompressedInputStream.java b/src/main/java/htsjdk/samtools/util/BlockCompressedInputStream.java index d384359fb1..898b15c495 100755 --- a/src/main/java/htsjdk/samtools/util/BlockCompressedInputStream.java +++ b/src/main/java/htsjdk/samtools/util/BlockCompressedInputStream.java @@ -29,6 +29,7 @@ import htsjdk.samtools.seekablestream.SeekableBufferedStream; import htsjdk.samtools.seekablestream.SeekableFileStream; import htsjdk.samtools.seekablestream.SeekableHTTPStream; +import htsjdk.samtools.seekablestream.SeekablePathStream; import htsjdk.samtools.seekablestream.SeekableStream; import htsjdk.samtools.util.zip.InflaterFactory; @@ -63,7 +64,7 @@ public class BlockCompressedInputStream extends InputStream implements LocationA private InputStream mStream = null; private boolean mIsClosed = false; - private SeekableStream mFile = null; // tsato: change name to mPath? + private SeekableStream mFile = null; private byte[] mFileBuffer = null; private DecompressedBlock mCurrentBlock = null; private int mCurrentOffset = 0; @@ -123,6 +124,15 @@ public BlockCompressedInputStream(final File file) throws IOException { this(file, BlockGunzipper.getDefaultInflaterFactory()); } + + /** + * Equivalent constructor for Path as the one that takes a File. Supports seeking. + */ + public BlockCompressedInputStream(final Path file) throws IOException { + this(new SeekablePathStream(file)); + } + + /** * Use this ctor if you wish to call seek() * @param file source of bytes @@ -135,6 +145,7 @@ public BlockCompressedInputStream(final File file, final InflaterFactory inflate blockGunzipper = new BlockGunzipper(inflaterFactory); } + /** * @param url source of bytes */ @@ -359,7 +370,7 @@ public void seek(final long pos) throws IOException { } // Cannot seek on streams that are not file based - if (mFile == null) { // tsato: mFile is a seekable stream--- + if (mFile == null) { throw new IOException(CANNOT_SEEK_STREAM_MSG); } @@ -706,7 +717,7 @@ static void readFully(SeekableByteChannel channel, ByteBuffer dst) throws IOExce @Deprecated public static void assertNonDefectiveFile(final File file) throws IOException { - assertNonDefectivePath(file.toPath()); + assertNonDefectivePath(IOUtil.toPath(file)); } public static void assertNonDefectivePath(final Path file) throws IOException { diff --git a/src/test/java/htsjdk/samtools/BamFileIoUtilsUnitTest.java b/src/test/java/htsjdk/samtools/BamFileIoUtilsUnitTest.java index 645a134954..14465a90e9 100644 --- a/src/test/java/htsjdk/samtools/BamFileIoUtilsUnitTest.java +++ b/src/test/java/htsjdk/samtools/BamFileIoUtilsUnitTest.java @@ -10,7 +10,6 @@ import java.io.File; import java.io.IOException; -import java.io.InputStream; import java.io.OutputStream; import java.nio.file.Files; import java.nio.file.Path; @@ -20,9 +19,8 @@ public class BamFileIoUtilsUnitTest extends HtsjdkTest { final static String TEST_DATA_DIR = "src/test/resources/htsjdk/samtools/cram/"; final static String NA12878_8000 = TEST_DATA_DIR + "CEUTrio.HiSeq.WGS.b37.NA12878.20.first.8000.bam"; + final static String NA12878_500 = TEST_DATA_DIR + "CEUTrio.HiSeq.WGS.b37.NA12878.20.first.500.bam"; final static String NA12878_20_21 = TEST_DATA_DIR + "NA12878.20.21.unmapped.orig.bam"; - final static int DEFAULT_NUM_RECORDS_TO_COMPARE = 10; - @DataProvider(name="ReheaderBamFileTestInput") public Object[][] getReheaderBamFileTestInput() { // tsato: is this the right naming scheme? e.g. get(method-name)Input @@ -37,8 +35,8 @@ public Object[][] getReheaderBamFileTestInput() { // tsato: is this the right na @Test(dataProvider = "ReheaderBamFileTestInput") public void testReheaderBamFile(final boolean createMd5, final boolean createIndex) throws IOException { - final File originalBam = new File(NA12878_8000); - SAMFileHeader header = SamReaderFactory.make().getFileHeader(new File(NA12878_8000)); + final File originalBam = new File(NA12878_500); + SAMFileHeader header = SamReaderFactory.make().getFileHeader(new File(NA12878_500)); header.addComment("This is a new, modified header"); final Path output = Files.createTempFile("output", ".bam"); @@ -48,8 +46,9 @@ public void testReheaderBamFile(final boolean createMd5, final boolean createInd final SamReader outputReader = SamReaderFactory.make().open(output); Assert.assertEquals(outputReader.getFileHeader(), header); - // Confirm that the reads are the same as the original - Assert.assertTrue(compareBamReads(originalBam, output.toFile(), DEFAULT_NUM_RECORDS_TO_COMPARE)); // tsato: toFile a stop gap + // Check that the reads are the same as the original + // tsato: should I be using something similar to IOUtil.toPath for converting Path -> File to propagate null? + assertBamRecordsEqual(originalBam, output.toFile()); if (createMd5){ Assert.assertTrue(Files.exists(output.resolveSibling(output.getFileName() + FileExtensions.MD5))); @@ -64,30 +63,18 @@ public void testReheaderBamFile(final boolean createMd5, final boolean createInd * Compare first (numRecordsToCompare) reads of two bam files. * We do not check for equality of the headers. * - * @param numRecordsToCompare the number of reads to compare - * * @return true if the first (numRecordsToCompare) reads are equal, else false */ - private boolean compareBamReads(final File bam1, final File bam2, final int numRecordsToCompare){ - SamReader reader = SamReaderFactory.make().open(bam1.toPath()); - final Iterator originalBamIterator = SamReaderFactory.make().open(bam1.toPath()).iterator(); - final Iterator outputBamIterator = SamReaderFactory.make().open(bam2.toPath()).iterator(); - - // tsato: per Louis...somehow failing the tests - boolean louis = false; - if (louis){ + private void assertBamRecordsEqual(final File bam1, final File bam2){ + try (SamReader reader1 = SamReaderFactory.make().open(bam1); + SamReader reader2 = SamReaderFactory.make().open(bam2)) { + final Iterator originalBamIterator = reader1.iterator(); + final Iterator outputBamIterator = reader2.iterator(); + Assert.assertEquals(originalBamIterator, outputBamIterator); + } catch (Exception e){ + throw new HtsjdkException("Encountered an error reading bam files: " + bam1.getAbsolutePath() + " and " + bam2.getAbsolutePath(), e); } - for (int i = 0; i < numRecordsToCompare; i++){ - final SAMRecord originalRead = originalBamIterator.next(); - final SAMRecord outputRead = outputBamIterator.next(); - if (! originalRead.equals(outputRead)){ - return false; - } - } - - - return true; } @DataProvider(name="BlockCopyBamFileTestInput") @@ -102,7 +89,6 @@ public Object[][] getBlockCopyBamFileTestInput() { @Test(dataProvider = "BlockCopyBamFileTestInput") public void testBlockCopyBamFile(final boolean skipHeader, final boolean skipTerminator) throws IOException { - System.out.println(skipHeader + ", " + skipTerminator); final File output = File.createTempFile("output", ".bam"); try (final OutputStream out = Files.newOutputStream(output.toPath())) { final Path input = Paths.get(NA12878_8000); @@ -118,7 +104,7 @@ public void testBlockCopyBamFile(final boolean skipHeader, final boolean skipTer } else { Assert.assertEquals(outputReader.getFileHeader(), inputReader.getFileHeader()); // Reading will fail when the header is absent - Assert.assertTrue(compareBamReads(input.toFile(), output, DEFAULT_NUM_RECORDS_TO_COMPARE)); + assertBamRecordsEqual(input.toFile(), output); } if (skipTerminator) { diff --git a/src/test/java/htsjdk/samtools/HtsjdkTestUtils.java b/src/test/java/htsjdk/samtools/HtsjdkTestUtils.java index 7d1c51f83d..90861af7e8 100644 --- a/src/test/java/htsjdk/samtools/HtsjdkTestUtils.java +++ b/src/test/java/htsjdk/samtools/HtsjdkTestUtils.java @@ -5,6 +5,7 @@ public class HtsjdkTestUtils { // tsato: change to HtsPath... public static final String TEST_DATA_DIR = "src/test/resources/htsjdk/samtools/cram/"; + // tsato: silly to use HtsPath when I know it can't handle e.g. gcloud files? public static final HtsPath NA12878_8000 = new HtsPath(TEST_DATA_DIR + "CEUTrio.HiSeq.WGS.b37.NA12878.20.first.8000.bam"); - public static final String NA12878_20_21 = TEST_DATA_DIR + "NA12878.20.21.unmapped.orig.bam"; + public static final HtsPath NA12878_20_21 = new HtsPath(TEST_DATA_DIR + 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