diff --git a/conf/hmf_genomes.config b/conf/hmf_genomes.config index 72c86930..1e2998f8 100644 --- a/conf/hmf_genomes.config +++ b/conf/hmf_genomes.config @@ -10,20 +10,20 @@ params { genomes { 'GRCh37_hmf' { - fasta = "https://pub-cf6ba01919994c3cbd354659947f74d8.r2.dev/genomes/GRCh37_hmf/24.0/Homo_sapiens.GRCh37.GATK.illumina.fasta" - fai = "https://pub-cf6ba01919994c3cbd354659947f74d8.r2.dev/genomes/GRCh37_hmf/24.0/samtools_index/1.16/Homo_sapiens.GRCh37.GATK.illumina.fasta.fai" - dict = "https://pub-cf6ba01919994c3cbd354659947f74d8.r2.dev/genomes/GRCh37_hmf/24.0/samtools_index/1.16/Homo_sapiens.GRCh37.GATK.illumina.fasta.dict" - bwa_index = "https://pub-cf6ba01919994c3cbd354659947f74d8.r2.dev/genomes/GRCh37_hmf/24.1/bwa_index/2.2.1.tar.gz" - gridss_index = "https://pub-cf6ba01919994c3cbd354659947f74d8.r2.dev/genomes/GRCh37_hmf/24.1/gridss_index/2.13.2.tar.gz" - star_index = "https://pub-cf6ba01919994c3cbd354659947f74d8.r2.dev/genomes/GRCh37_hmf/24.0/star_index/gencode_19/2.7.3a.tar.gz" + fasta = "https://pub-cf6ba01919994c3cbd354659947f74d8.r2.dev/genomes/GRCh37_hmf/24.0/Homo_sapiens.GRCh37.GATK.illumina.fasta" + fai = "https://pub-cf6ba01919994c3cbd354659947f74d8.r2.dev/genomes/GRCh37_hmf/24.0/samtools_index/1.16/Homo_sapiens.GRCh37.GATK.illumina.fasta.fai" + dict = "https://pub-cf6ba01919994c3cbd354659947f74d8.r2.dev/genomes/GRCh37_hmf/24.0/samtools_index/1.16/Homo_sapiens.GRCh37.GATK.illumina.fasta.dict" + bwamem2_index = "https://pub-cf6ba01919994c3cbd354659947f74d8.r2.dev/genomes/GRCh37_hmf/24.1/bwa-mem2_index/2.2.1.tar.gz" + gridss_index = "https://pub-cf6ba01919994c3cbd354659947f74d8.r2.dev/genomes/GRCh37_hmf/24.1/gridss_index/2.13.2.tar.gz" + star_index = "https://pub-cf6ba01919994c3cbd354659947f74d8.r2.dev/genomes/GRCh37_hmf/24.0/star_index/gencode_19/2.7.3a.tar.gz" } 'GRCh38_hmf' { - fasta = "https://pub-cf6ba01919994c3cbd354659947f74d8.r2.dev/genomes/GRCh38_hmf/24.0/GCA_000001405.15_GRCh38_no_alt_analysis_set.fna" - fai = "https://pub-cf6ba01919994c3cbd354659947f74d8.r2.dev/genomes/GRCh38_hmf/24.0/samtools_index/1.16/GCA_000001405.15_GRCh38_no_alt_analysis_set.fna.fai" - dict = "https://pub-cf6ba01919994c3cbd354659947f74d8.r2.dev/genomes/GRCh38_hmf/24.0/samtools_index/1.16/GCA_000001405.15_GRCh38_no_alt_analysis_set.fna.dict" - bwa_index = "https://pub-cf6ba01919994c3cbd354659947f74d8.r2.dev/genomes/GRCh38_hmf/24.1/bwa_index/2.2.1.tar.gz" - gridss_index = "https://pub-cf6ba01919994c3cbd354659947f74d8.r2.dev/genomes/GRCh38_hmf/24.1/gridss_index/2.13.2.tar.gz" - star_index = "https://pub-cf6ba01919994c3cbd354659947f74d8.r2.dev/genomes/GRCh38_hmf/24.0/star_index/gencode_38/2.7.3a.tar.gz" + fasta = "https://pub-cf6ba01919994c3cbd354659947f74d8.r2.dev/genomes/GRCh38_hmf/24.0/GCA_000001405.15_GRCh38_no_alt_analysis_set.fna" + fai = "https://pub-cf6ba01919994c3cbd354659947f74d8.r2.dev/genomes/GRCh38_hmf/24.0/samtools_index/1.16/GCA_000001405.15_GRCh38_no_alt_analysis_set.fna.fai" + dict = "https://pub-cf6ba01919994c3cbd354659947f74d8.r2.dev/genomes/GRCh38_hmf/24.0/samtools_index/1.16/GCA_000001405.15_GRCh38_no_alt_analysis_set.fna.dict" + bwamem2_index = "https://pub-cf6ba01919994c3cbd354659947f74d8.r2.dev/genomes/GRCh38_hmf/24.1/bwa-mem2_index/2.2.1.tar.gz" + gridss_index = "https://pub-cf6ba01919994c3cbd354659947f74d8.r2.dev/genomes/GRCh38_hmf/24.1/gridss_index/2.13.2.tar.gz" + star_index = "https://pub-cf6ba01919994c3cbd354659947f74d8.r2.dev/genomes/GRCh38_hmf/24.0/star_index/gencode_38/2.7.3a.tar.gz" } } } diff --git a/conf/test_stub.config b/conf/test_stub.config index b0a99ed0..ee1d5d7a 100644 --- a/conf/test_stub.config +++ b/conf/test_stub.config @@ -26,12 +26,12 @@ params { genomes { 'GRCh38_hmf' { - fasta = "temp/GRCh38.fasta" - fai = "temp/GRCh38.fai" - dict = "temp/GRCh38.dict" - bwa_index = "temp/GRCh38_bwa-mem2_index/" - gridss_index = "temp/GRCh38_gridss_index/" - star_index = "temp/GRCh38_star_index/" + fasta = "temp/GRCh38.fasta" + fai = "temp/GRCh38.fai" + dict = "temp/GRCh38.dict" + bwamem2_index = "temp/GRCh38_bwa-mem2_index/" + gridss_index = "temp/GRCh38_gridss_index/" + star_index = "temp/GRCh38_star_index/" } } diff --git a/docs/usage.md b/docs/usage.md index ffff4e08..add8c752 100644 --- a/docs/usage.md +++ b/docs/usage.md @@ -334,7 +334,7 @@ params { fasta = "/path/to/GCA_000001405.15_GRCh38_no_alt_analysis_set.fna" fai = "/path/to/GCA_000001405.15_GRCh38_no_alt_analysis_set.fna.fai" dict = "/path/to/GCA_000001405.15_GRCh38_no_alt_analysis_set.fna.dict" - bwa_index = "/path/to/bwa-mem2_index/" + bwamem2_index = "/path/to/bwa-mem2_index/" gridss_index = "/path/to/gridss_index/" star_index = "/path/to/star_index/" } @@ -357,7 +357,7 @@ _GRCh37 genome (Hartwig) [`GRCh37_hmf`]_ | FASTA | [Homo_sapiens.GRCh37.GATK.illumina.fasta](https://pub-cf6ba01919994c3cbd354659947f74d8.r2.dev/genomes/GRCh37_hmf/24.0/Homo_sapiens.GRCh37.GATK.illumina.fasta) | | FASTA index | [Homo_sapiens.GRCh37.GATK.illumina.fasta.fai](https://pub-cf6ba01919994c3cbd354659947f74d8.r2.dev/genomes/GRCh37_hmf/24.0/samtools_index/1.16/Homo_sapiens.GRCh37.GATK.illumina.fasta.fai) | | FASTA seq dictionary | [Homo_sapiens.GRCh37.GATK.illumina.fasta.dict](https://pub-cf6ba01919994c3cbd354659947f74d8.r2.dev/genomes/GRCh37_hmf/24.0/samtools_index/1.16/Homo_sapiens.GRCh37.GATK.illumina.fasta.dict) | -| bwa-mem2 index | [bwa_index/2.2.1.tar.gz](https://pub-cf6ba01919994c3cbd354659947f74d8.r2.dev/genomes/GRCh37_hmf/24.1/bwa_index/2.2.1.tar.gz) | +| bwa-mem2 index | [bwa-mem2_index/2.2.1.tar.gz](https://pub-cf6ba01919994c3cbd354659947f74d8.r2.dev/genomes/GRCh37_hmf/24.1/bwa-mem2_index/2.2.1.tar.gz) | | GRIDSS index | [gridss_index/2.13.2.tar.gz](https://pub-cf6ba01919994c3cbd354659947f74d8.r2.dev/genomes/GRCh37_hmf/24.1/gridss_index/2.13.2.tar.gz) | | STAR index | [star_index/gencode_19/2.7.3a.tar.gz](https://pub-cf6ba01919994c3cbd354659947f74d8.r2.dev/genomes/GRCh37_hmf/24.0/star_index/gencode_19/2.7.3a.tar.gz) | @@ -368,7 +368,7 @@ _GRCh38 genome (Hartwig) [`GRCh38_hmf`]_ | FASTA | [GCA_000001405.15_GRCh38_no_alt_analysis_set.fna](https://pub-cf6ba01919994c3cbd354659947f74d8.r2.dev/genomes/GRCh38_hmf/24.0/GCA_000001405.15_GRCh38_no_alt_analysis_set.fna) | | FASTA index | [GCA_000001405.15_GRCh38_no_alt_analysis_set.fna.fai](https://pub-cf6ba01919994c3cbd354659947f74d8.r2.dev/genomes/GRCh38_hmf/24.0/samtools_index/1.16/GCA_000001405.15_GRCh38_no_alt_analysis_set.fna.fai) | | FASTA seq dictionary | [GCA_000001405.15_GRCh38_no_alt_analysis_set.fna.dict](https://pub-cf6ba01919994c3cbd354659947f74d8.r2.dev/genomes/GRCh38_hmf/24.0/samtools_index/1.16/GCA_000001405.15_GRCh38_no_alt_analysis_set.fna.dict) | -| bwa-mem2 index | [bwa_index/2.2.1.tar.gz](https://pub-cf6ba01919994c3cbd354659947f74d8.r2.dev/genomes/GRCh38_hmf/24.1/bwa_index/2.2.1.tar.gz) | +| bwa-mem2 index | [bwa-mem2_index/2.2.1.tar.gz](https://pub-cf6ba01919994c3cbd354659947f74d8.r2.dev/genomes/GRCh38_hmf/24.1/bwa-mem2_index/2.2.1.tar.gz) | | GRIDSS index | [gridss_index/2.13.2.tar.gz](https://pub-cf6ba01919994c3cbd354659947f74d8.r2.dev/genomes/GRCh38_hmf/24.1/gridss_index/2.13.2.tar.gz) | | STAR index | [star_index/gencode_38/2.7.3a.tar.gz](https://pub-cf6ba01919994c3cbd354659947f74d8.r2.dev/genomes/GRCh38_hmf/24.0/star_index/gencode_38/2.7.3a.tar.gz) | diff --git a/lib/Utils.groovy b/lib/Utils.groovy index dba43220..0601ed8f 100644 --- a/lib/Utils.groovy +++ b/lib/Utils.groovy @@ -197,12 +197,13 @@ class Utils { def fps = [ params.ref_data_genome_alt, - params.ref_data_genome_bwa_index, + params.ref_data_genome_bwamem2_index, params.ref_data_genome_dict, params.ref_data_genome_fai, params.ref_data_genome_fasta, params.ref_data_genome_gridss_index, params.ref_data_genome_gtf, + params.ref_data_genome_star_index, params.ref_data_virusbreakenddb_path, ] @@ -323,7 +324,7 @@ class Utils { def has_alt_contigs = params.genome_type == 'alt' // Ensure that custom genomes with ALT contigs that need indexes built have the required .alt file - def has_bwa_indexes = (params.ref_data_genome_bwa_index && params.ref_data_genome_gridss_index) + def has_bwa_indexes = (params.ref_data_genome_bwamem2_index && params.ref_data_genome_gridss_index) def has_alt_file = params.containsKey('ref_data_genome_alt') && params.ref_data_genome_alt def run_bwa_or_gridss_index = run_config.stages.alignment && run_config.has_dna_fastq && !has_bwa_indexes diff --git a/main.nf b/main.nf index e91f8199..7fdb0419 100644 --- a/main.nf +++ b/main.nf @@ -31,12 +31,12 @@ include { getGenomeAttribute } from './subworkflows/local/utils_nfcore_oncoanaly ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ */ -params.ref_data_genome_fasta = getGenomeAttribute('fasta') -params.ref_data_genome_fai = getGenomeAttribute('fai') -params.ref_data_genome_dict = getGenomeAttribute('dict') -params.ref_data_genome_bwa_index = getGenomeAttribute('bwa_index') -params.ref_data_genome_gridss_index = getGenomeAttribute('gridss_index') -params.ref_data_genome_star_index = getGenomeAttribute('star_index') +params.ref_data_genome_fasta = getGenomeAttribute('fasta') +params.ref_data_genome_fai = getGenomeAttribute('fai') +params.ref_data_genome_dict = getGenomeAttribute('dict') +params.ref_data_genome_bwamem2_index = getGenomeAttribute('bwamem2_index') +params.ref_data_genome_gridss_index = getGenomeAttribute('gridss_index') +params.ref_data_genome_star_index = getGenomeAttribute('star_index') WorkflowMain.setParamsDefaults(params, log) WorkflowMain.validateParams(params, log) diff --git a/modules/local/bwa-mem2/mem/main.nf b/modules/local/bwa-mem2/mem/main.nf index 8a5b8046..d1c9ba81 100644 --- a/modules/local/bwa-mem2/mem/main.nf +++ b/modules/local/bwa-mem2/mem/main.nf @@ -10,7 +10,7 @@ process BWAMEM2_ALIGN { input: tuple val(meta), path(reads_fwd), path(reads_rev) path genome_fasta - path genome_bwa_index + path genome_bwamem2_index output: tuple val(meta), path('*.bam'), path('*.bai'), emit: bam @@ -28,7 +28,7 @@ process BWAMEM2_ALIGN { def output_fn = meta.split ? "${meta.split}.${meta.sample_id}.${meta.read_group}.bam" : "${meta.sample_id}.${meta.read_group}.bam" """ - ln -fs \$(find -L ${genome_bwa_index} -type f) ./ + ln -fs \$(find -L ${genome_bwamem2_index} -type f) ./ bwa-mem2 mem \\ ${args} \\ diff --git a/modules/local/bwa-mem2/mem/meta.yml b/modules/local/bwa-mem2/mem/meta.yml index af6d50a9..da61d816 100644 --- a/modules/local/bwa-mem2/mem/meta.yml +++ b/modules/local/bwa-mem2/mem/meta.yml @@ -29,9 +29,9 @@ input: type: file description: Reference genome assembly FASTA file pattern: "*.{fa,fasta}" - - genome_bwa_index: + - genome_bwamem2_index: type: directory - description: bwa-mem1 index directory + description: bwa-mem2 index directory output: - meta: type: map diff --git a/modules/local/custom/lilac_extract_and_index_contig/main.nf b/modules/local/custom/lilac_extract_and_index_contig/main.nf index b8e6bb75..a9afd212 100644 --- a/modules/local/custom/lilac_extract_and_index_contig/main.nf +++ b/modules/local/custom/lilac_extract_and_index_contig/main.nf @@ -15,7 +15,7 @@ process CUSTOM_EXTRACTCONTIG { output: path "*extracted.fa" , emit: contig - path "*extracted.fa.*", emit: bwa_index + path "*extracted.fa.*", emit: bwamem2_index path 'versions.yml' , emit: versions when: diff --git a/nextflow.config b/nextflow.config index f76c321a..656b89bb 100644 --- a/nextflow.config +++ b/nextflow.config @@ -78,7 +78,7 @@ params { 'panel_data_paths', 'ref_data', 'ref_data_genome_alt', - 'ref_data_genome_bwa_index', + 'ref_data_genome_bwamem2_index', 'ref_data_genome_dict', 'ref_data_genome_fai', 'ref_data_genome_fasta', diff --git a/subworkflows/local/lilac_calling/main.nf b/subworkflows/local/lilac_calling/main.nf index f267902e..62e43abe 100644 --- a/subworkflows/local/lilac_calling/main.nf +++ b/subworkflows/local/lilac_calling/main.nf @@ -131,7 +131,7 @@ workflow LILAC_CALLING { REALIGNREADS( SLICEBAM.out.bam, EXTRACTCONTIG.out.contig, - EXTRACTCONTIG.out.bwa_index, + EXTRACTCONTIG.out.bwamem2_index, ) ch_versions = ch_versions.mix(REALIGNREADS.out.versions) diff --git a/subworkflows/local/prepare_reference/main.nf b/subworkflows/local/prepare_reference/main.nf index 121733a1..85f5366b 100644 --- a/subworkflows/local/prepare_reference/main.nf +++ b/subworkflows/local/prepare_reference/main.nf @@ -55,28 +55,28 @@ workflow PREPARE_REFERENCE { // // Set bwa-mem2 index, unpack or create if required // - ch_genome_bwa_index = Channel.empty() + ch_genome_bwamem2_index = Channel.empty() if (run_config.has_dna && run_config.stages.alignment) { - if (!params.ref_data_genome_bwa_index) { + if (!params.ref_data_genome_bwamem2_index) { BWAMEM2_INDEX( ch_genome_fasta, params.ref_data_genome_alt ? file(params.ref_data_genome_alt) : [], ) - ch_genome_bwa_index = BWAMEM2_INDEX.out.index + ch_genome_bwamem2_index = BWAMEM2_INDEX.out.index ch_versions = ch_versions.mix(BWAMEM2_INDEX.out.versions) - } else if (params.ref_data_genome_bwa_index.endsWith('.tar.gz')) { + } else if (params.ref_data_genome_bwamem2_index.endsWith('.tar.gz')) { - ch_genome_bwa_index_inputs = Channel.fromPath(params.ref_data_genome_bwa_index) + ch_genome_bwamem2_index_inputs = Channel.fromPath(params.ref_data_genome_bwamem2_index) .map { [[id: "bwa-mem2_index_${it.name.replaceAll('\\.tar\\.gz$', '')}"], it] } - DECOMP_BWAMEM2_INDEX(ch_genome_bwa_index_inputs) - ch_genome_bwa_index = DECOMP_BWAMEM2_INDEX.out.dir + DECOMP_BWAMEM2_INDEX(ch_genome_bwamem2_index_inputs) + ch_genome_bwamem2_index = DECOMP_BWAMEM2_INDEX.out.dir } else { - ch_genome_bwa_index = getRefFileChannel('ref_data_genome_bwa_index') + ch_genome_bwamem2_index = getRefFileChannel('ref_data_genome_bwamem2_index') } } @@ -235,7 +235,7 @@ workflow PREPARE_REFERENCE { ch_genome_fasta, ch_genome_fai, ch_genome_dict, - ch_genome_bwa_index, + ch_genome_bwamem2_index, ch_genome_gridss_index, ch_genome_star_index, ch_virusbreakenddb, @@ -258,19 +258,19 @@ workflow PREPARE_REFERENCE { } emit: - genome_fasta = ch_genome_fasta.first() // path: genome_fasta - genome_fai = ch_genome_fai.first() // path: genome_fai - genome_dict = ch_genome_dict.first() // path: genome_dict - genome_bwa_index = ch_genome_bwa_index.first() // path: genome_bwa_index - genome_gridss_index = ch_genome_gridss_index.first() // path: genome_gridss_index - genome_star_index = ch_genome_star_index.first() // path: genome_star_index - genome_version = ch_genome_version // val: genome_version - - virusbreakenddb = ch_virusbreakenddb.first() // path: VIRUSBreakend database - hmf_data = ch_hmf_data // map: HMF data paths - panel_data = ch_panel_data // map: Panel data paths - - versions = ch_versions // channel: [ versions.yml ] + genome_fasta = ch_genome_fasta.first() // path: genome_fasta + genome_fai = ch_genome_fai.first() // path: genome_fai + genome_dict = ch_genome_dict.first() // path: genome_dict + genome_bwamem2_index = ch_genome_bwamem2_index.first() // path: genome_bwa-mem2_index + genome_gridss_index = ch_genome_gridss_index.first() // path: genome_gridss_index + genome_star_index = ch_genome_star_index.first() // path: genome_star_index + genome_version = ch_genome_version // val: genome_version + + virusbreakenddb = ch_virusbreakenddb.first() // path: VIRUSBreakend database + hmf_data = ch_hmf_data // map: HMF data paths + panel_data = ch_panel_data // map: Panel data paths + + versions = ch_versions // channel: [ versions.yml ] } def getRefFileChannel(key) { diff --git a/subworkflows/local/read_alignment_dna/main.nf b/subworkflows/local/read_alignment_dna/main.nf index 6632f921..795bdc73 100644 --- a/subworkflows/local/read_alignment_dna/main.nf +++ b/subworkflows/local/read_alignment_dna/main.nf @@ -15,7 +15,7 @@ workflow READ_ALIGNMENT_DNA { // Reference data genome_fasta // channel: [mandatory] /path/to/genome_fasta - genome_bwa_index // channel: [mandatory] /path/to/genome_bwa_index_dir/ + genome_bwamem2_index // channel: [mandatory] /path/to/genome_bwa-mem2_index_dir/ // Params max_fastq_records // numeric: [mandatory] max number of FASTQ records per split @@ -128,11 +128,11 @@ workflow READ_ALIGNMENT_DNA { // MODULE: BWA-MEM2 // // Create process input channel - // channel: [ meta_bwa, fastq_fwd, fastq_rev ] - ch_bwa_inputs = ch_fastqs_ready + // channel: [ meta_bwamem2, fastq_fwd, fastq_rev ] + ch_bwamem2_inputs = ch_fastqs_ready .map { meta_fastq_ready, fastq_fwd, fastq_rev -> - def meta_bwa = [ + def meta_bwamem2 = [ *:meta_fastq_ready, @@ -142,14 +142,14 @@ workflow READ_ALIGNMENT_DNA { ] - return [meta_bwa, fastq_fwd, fastq_rev] + return [meta_bwamem2, fastq_fwd, fastq_rev] } // Run process BWAMEM2_ALIGN( - ch_bwa_inputs, + ch_bwamem2_inputs, genome_fasta, - genome_bwa_index, + genome_bwamem2_index, ) ch_versions = ch_versions.mix(BWAMEM2_ALIGN.out.versions) @@ -157,25 +157,25 @@ workflow READ_ALIGNMENT_DNA { // Reunite BAMs // First, count expected BAMs per sample for non-blocking groupTuple op // channel: [ meta_count, group_size ] - ch_sample_fastq_counts = ch_bwa_inputs - .map { meta_bwa, reads_fwd, reads_rev -> + ch_sample_fastq_counts = ch_bwamem2_inputs + .map { meta_bwamem2, reads_fwd, reads_rev -> def meta_count = [ - key: meta_bwa.key, - sample_type: meta_bwa.sample_type, + key: meta_bwamem2.key, + sample_type: meta_bwamem2.sample_type, ] - return [meta_count, meta_bwa] + return [meta_count, meta_bwamem2] } .groupTuple() - .map { meta_count, meta_bwas -> return [meta_count, meta_bwas.size()] } + .map { meta_count, metas_bwamem2 -> return [meta_count, metas_bwamem2.size()] } // Now, group with expected size then sort into tumor and normal channels // channel: [ meta_group, [bam, ...], [bai, ...] ] ch_bams_united = ch_sample_fastq_counts .cross( // First element to match meta_count above for `cross` - BWAMEM2_ALIGN.out.bam.map { meta_bwa, bam, bai -> [[key: meta_bwa.key, sample_type: meta_bwa.sample_type], bam, bai] } + BWAMEM2_ALIGN.out.bam.map { meta_bwamem2, bam, bai -> [[key: meta_bwamem2.key, sample_type: meta_bwamem2.sample_type], bam, bai] } ) .map { count_tuple, bam_tuple -> diff --git a/workflows/targeted.nf b/workflows/targeted.nf index 1c8c5b01..c6193c2f 100644 --- a/workflows/targeted.nf +++ b/workflows/targeted.nf @@ -114,7 +114,7 @@ workflow TARGETED { READ_ALIGNMENT_DNA( ch_inputs, ref_data.genome_fasta, - ref_data.genome_bwa_index, + ref_data.genome_bwamem2_index, params.max_fastq_records, ) diff --git a/workflows/wgts.nf b/workflows/wgts.nf index b2d5096b..188ff68c 100644 --- a/workflows/wgts.nf +++ b/workflows/wgts.nf @@ -123,7 +123,7 @@ workflow WGTS { READ_ALIGNMENT_DNA( ch_inputs, ref_data.genome_fasta, - ref_data.genome_bwa_index, + ref_data.genome_bwamem2_index, params.max_fastq_records, )