From 3132087ffa20bfeee426c102e3fdd6d7ffeb783c Mon Sep 17 00:00:00 2001 From: Nicolas Vannieuwkerke Date: Wed, 20 Dec 2023 10:52:20 +0100 Subject: [PATCH 01/64] Add summary to readme --- README.md | 12 ++++++++++-- 1 file changed, 10 insertions(+), 2 deletions(-) diff --git a/README.md b/README.md index 40255f9..68efb06 100644 --- a/README.md +++ b/README.md @@ -21,8 +21,16 @@ workflows use the "tube map" design for that. See https://nf-co.re/docs/contributing/design_guidelines#examples for examples. --> -1. Read QC ([`FastQC`](https://www.bioinformatics.babraham.ac.uk/projects/fastqc/)) -2. Present QC for raw reads ([`MultiQC`](http://multiqc.info/)) +## Pipeline Summary + +1. Gender prediction +2. CRAM -> BAM +3. ExomeDepth counting per sample +4. Create count file for all samples per pool +5. ExomeDepth CNV calling per sample +6. Convert BED to VCF +7. Annotation per sample (VEP + custom annotation) => make it possible to run from here (from VCF file) +8. Output as XLSX file ## Usage From dfbc90ae0f6356b36d4bfb9d2d8046aa7d74354d Mon Sep 17 00:00:00 2001 From: Nicolas Vannieuwkerke Date: Wed, 20 Dec 2023 11:38:42 +0100 Subject: [PATCH 02/64] Fix issues in the template --- assets/schema_input.json | 3 +- ...cnv.groovy => WorkflowCmggExomecnv.groovy} | 2 +- main.nf | 8 ++-- nextflow_schema.json | 1 + subworkflows/local/input_check.nf | 44 ------------------- .../{nf-cmgg-exomecnv.nf => cmggexomecnv.nf} | 21 +++------ 6 files changed, 15 insertions(+), 64 deletions(-) rename lib/{WorkflowNf-cmgg-exomecnv.groovy => WorkflowCmggExomecnv.groovy} (99%) delete mode 100644 subworkflows/local/input_check.nf rename workflows/{nf-cmgg-exomecnv.nf => cmggexomecnv.nf} (82%) diff --git a/assets/schema_input.json b/assets/schema_input.json index c84558d..cc71c7a 100644 --- a/assets/schema_input.json +++ b/assets/schema_input.json @@ -10,7 +10,8 @@ "sample": { "type": "string", "pattern": "^\\S+$", - "errorMessage": "Sample name must be provided and cannot contain spaces" + "errorMessage": "Sample name must be provided and cannot contain spaces", + "meta": ["id"] }, "fastq_1": { "type": "string", diff --git a/lib/WorkflowNf-cmgg-exomecnv.groovy b/lib/WorkflowCmggExomecnv.groovy similarity index 99% rename from lib/WorkflowNf-cmgg-exomecnv.groovy rename to lib/WorkflowCmggExomecnv.groovy index 8e4da88..23c36ca 100755 --- a/lib/WorkflowNf-cmgg-exomecnv.groovy +++ b/lib/WorkflowCmggExomecnv.groovy @@ -5,7 +5,7 @@ import nextflow.Nextflow import groovy.text.SimpleTemplateEngine -class WorkflowNf-cmgg-exomecnv { +class WorkflowCmggExomecnv { // // Check and validate parameters diff --git a/main.nf b/main.nf index 007a53d..3b699df 100644 --- a/main.nf +++ b/main.nf @@ -50,13 +50,13 @@ WorkflowMain.initialise(workflow, params, log) ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ */ -include { NF-CMGG-EXOMECNV } from './workflows/nf-cmgg-exomecnv' +include { CMGGEXOMECNV } from './workflows/cmggexomecnv' // // WORKFLOW: Run main CenterForMedicalGeneticsGhent/nf-cmgg-exomecnv analysis pipeline // -workflow CENTERFORMEDICALGENETICSGHENT_NF-CMGG-EXOMECNV { - NF-CMGG-EXOMECNV () +workflow CMGG_CMGGEXOMECNV { + CMGGEXOMECNV () } /* @@ -70,7 +70,7 @@ workflow CENTERFORMEDICALGENETICSGHENT_NF-CMGG-EXOMECNV { // See: https://github.com/nf-core/rnaseq/issues/619 // workflow { - CENTERFORMEDICALGENETICSGHENT_NF-CMGG-EXOMECNV () + CMGG_CMGGEXOMECNV () } /* diff --git a/nextflow_schema.json b/nextflow_schema.json index 860d68e..542fb8f 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -18,6 +18,7 @@ "exists": true, "mimetype": "text/csv", "pattern": "^\\S+\\.csv$", + "schema": "assets/schema_input.json", "description": "Path to comma-separated file containing information about the samples in the experiment.", "help_text": "You will need to create a design file with information about the samples in your experiment before running the pipeline. Use this parameter to specify its location. It has to be a comma-separated file with 3 columns, and a header row.", "fa_icon": "fas fa-file-csv" diff --git a/subworkflows/local/input_check.nf b/subworkflows/local/input_check.nf deleted file mode 100644 index 0aecf87..0000000 --- a/subworkflows/local/input_check.nf +++ /dev/null @@ -1,44 +0,0 @@ -// -// Check input samplesheet and get read channels -// - -include { SAMPLESHEET_CHECK } from '../../modules/local/samplesheet_check' - -workflow INPUT_CHECK { - take: - samplesheet // file: /path/to/samplesheet.csv - - main: - SAMPLESHEET_CHECK ( samplesheet ) - .csv - .splitCsv ( header:true, sep:',' ) - .map { create_fastq_channel(it) } - .set { reads } - - emit: - reads // channel: [ val(meta), [ reads ] ] - versions = SAMPLESHEET_CHECK.out.versions // channel: [ versions.yml ] -} - -// Function to get list of [ meta, [ fastq_1, fastq_2 ] ] -def create_fastq_channel(LinkedHashMap row) { - // create meta map - def meta = [:] - meta.id = row.sample - meta.single_end = row.single_end.toBoolean() - - // add path(s) of the fastq file(s) to the meta map - def fastq_meta = [] - if (!file(row.fastq_1).exists()) { - exit 1, "ERROR: Please check input samplesheet -> Read 1 FastQ file does not exist!\n${row.fastq_1}" - } - if (meta.single_end) { - fastq_meta = [ meta, [ file(row.fastq_1) ] ] - } else { - if (!file(row.fastq_2).exists()) { - exit 1, "ERROR: Please check input samplesheet -> Read 2 FastQ file does not exist!\n${row.fastq_2}" - } - fastq_meta = [ meta, [ file(row.fastq_1), file(row.fastq_2) ] ] - } - return fastq_meta -} diff --git a/workflows/nf-cmgg-exomecnv.nf b/workflows/cmggexomecnv.nf similarity index 82% rename from workflows/nf-cmgg-exomecnv.nf rename to workflows/cmggexomecnv.nf index 8cdfbf9..71dc4f2 100644 --- a/workflows/nf-cmgg-exomecnv.nf +++ b/workflows/cmggexomecnv.nf @@ -4,7 +4,7 @@ ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ */ -include { paramsSummaryLog; paramsSummaryMap } from 'plugin/nf-validation' +include { paramsSummaryLog; paramsSummaryMap; fromSamplesheet } from 'plugin/nf-validation' def logo = NfcoreTemplate.logo(workflow, params.monochrome_logs) def citation = '\n' + WorkflowMain.citation(workflow) + '\n' @@ -13,7 +13,7 @@ def summary_params = paramsSummaryMap(workflow) // Print parameter summary log to screen log.info logo + paramsSummaryLog(workflow) + citation -WorkflowNf-cmgg-exomecnv.initialise(params, log) +WorkflowCmggExomecnv.initialise(params, log) /* ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ @@ -35,7 +35,6 @@ ch_multiqc_custom_methods_description = params.multiqc_methods_description ? fil // // SUBWORKFLOW: Consisting of a mix of local and nf-core/modules // -include { INPUT_CHECK } from '../subworkflows/local/input_check' /* ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ @@ -59,26 +58,20 @@ include { CUSTOM_DUMPSOFTWAREVERSIONS } from '../modules/nf-core/custom/dumpsoft // Info required for completion email and summary def multiqc_report = [] -workflow NF-CMGG-EXOMECNV { +workflow CMGGEXOMECNV { ch_versions = Channel.empty() // // SUBWORKFLOW: Read in samplesheet, validate and stage input files // - INPUT_CHECK ( - file(params.input) - ) - ch_versions = ch_versions.mix(INPUT_CHECK.out.versions) - // TODO: OPTIONAL, you can use nf-validation plugin to create an input channel from the samplesheet with Channel.fromSamplesheet("input") - // See the documentation https://nextflow-io.github.io/nf-validation/samplesheets/fromSamplesheet/ - // ! There is currently no tooling to help you write a sample sheet schema + ch_input = Channel.fromSamplesheet("input") // // MODULE: Run FastQC // FASTQC ( - INPUT_CHECK.out.reads + ch_input ) ch_versions = ch_versions.mix(FASTQC.out.versions.first()) @@ -89,10 +82,10 @@ workflow NF-CMGG-EXOMECNV { // // MODULE: MultiQC // - workflow_summary = WorkflowNf-cmgg-exomecnv.paramsSummaryMultiqc(workflow, summary_params) + workflow_summary = WorkflowCmggExomecnv.paramsSummaryMultiqc(workflow, summary_params) ch_workflow_summary = Channel.value(workflow_summary) - methods_description = WorkflowNf-cmgg-exomecnv.methodsDescriptionText(workflow, ch_multiqc_custom_methods_description, params) + methods_description = WorkflowCmggExomecnv.methodsDescriptionText(workflow, ch_multiqc_custom_methods_description, params) ch_methods_description = Channel.value(methods_description) ch_multiqc_files = Channel.empty() From e6d8602b9f0b6c1f25e6d59ef0a3b3066e48a6f8 Mon Sep 17 00:00:00 2001 From: torossee Date: Fri, 2 Feb 2024 16:57:35 +0100 Subject: [PATCH 03/64] clean-up template + install samtools_convert --- bin/check_samplesheet.py | 259 ------------------ lib/WorkflowCmggExomecnv.groovy | 6 +- main.nf | 2 +- modules.json | 20 +- modules/nf-core/fastqc/main.nf | 55 ---- modules/nf-core/fastqc/meta.yml | 57 ---- modules/nf-core/fastqc/tests/main.nf.test | 41 --- .../nf-core/fastqc/tests/main.nf.test.snap | 10 - modules/nf-core/fastqc/tests/tags.yml | 2 - .../convert}/environment.yml | 4 +- modules/nf-core/samtools/convert/main.nf | 42 +++ modules/nf-core/samtools/convert/meta.yml | 55 ++++ .../samtools/convert/tests/main.nf.test | 82 ++++++ .../samtools/convert/tests/main.nf.test.snap | 22 ++ .../nf-core/samtools/convert/tests/tags.yml | 2 + nextflow.config | 12 +- nextflow_schema.json | 6 + .../bam_variant_calling_exomedepth/main.nf | 37 +++ workflows/cmggexomecnv.nf | 16 +- 19 files changed, 280 insertions(+), 450 deletions(-) delete mode 100755 bin/check_samplesheet.py delete mode 100644 modules/nf-core/fastqc/main.nf delete mode 100644 modules/nf-core/fastqc/meta.yml delete mode 100644 modules/nf-core/fastqc/tests/main.nf.test delete mode 100644 modules/nf-core/fastqc/tests/main.nf.test.snap delete mode 100644 modules/nf-core/fastqc/tests/tags.yml rename modules/nf-core/{fastqc => samtools/convert}/environment.yml (56%) create mode 100644 modules/nf-core/samtools/convert/main.nf create mode 100644 modules/nf-core/samtools/convert/meta.yml create mode 100644 modules/nf-core/samtools/convert/tests/main.nf.test create mode 100644 modules/nf-core/samtools/convert/tests/main.nf.test.snap create mode 100644 modules/nf-core/samtools/convert/tests/tags.yml create mode 100644 subworkflows/local/bam_variant_calling_exomedepth/main.nf diff --git a/bin/check_samplesheet.py b/bin/check_samplesheet.py deleted file mode 100755 index 4a758fe..0000000 --- a/bin/check_samplesheet.py +++ /dev/null @@ -1,259 +0,0 @@ -#!/usr/bin/env python - - -"""Provide a command line tool to validate and transform tabular samplesheets.""" - - -import argparse -import csv -import logging -import sys -from collections import Counter -from pathlib import Path - -logger = logging.getLogger() - - -class RowChecker: - """ - Define a service that can validate and transform each given row. - - Attributes: - modified (list): A list of dicts, where each dict corresponds to a previously - validated and transformed row. The order of rows is maintained. - - """ - - VALID_FORMATS = ( - ".fq.gz", - ".fastq.gz", - ) - - def __init__( - self, - sample_col="sample", - first_col="fastq_1", - second_col="fastq_2", - single_col="single_end", - **kwargs, - ): - """ - Initialize the row checker with the expected column names. - - Args: - sample_col (str): The name of the column that contains the sample name - (default "sample"). - first_col (str): The name of the column that contains the first (or only) - FASTQ file path (default "fastq_1"). - second_col (str): The name of the column that contains the second (if any) - FASTQ file path (default "fastq_2"). - single_col (str): The name of the new column that will be inserted and - records whether the sample contains single- or paired-end sequencing - reads (default "single_end"). - - """ - super().__init__(**kwargs) - self._sample_col = sample_col - self._first_col = first_col - self._second_col = second_col - self._single_col = single_col - self._seen = set() - self.modified = [] - - def validate_and_transform(self, row): - """ - Perform all validations on the given row and insert the read pairing status. - - Args: - row (dict): A mapping from column headers (keys) to elements of that row - (values). - - """ - self._validate_sample(row) - self._validate_first(row) - self._validate_second(row) - self._validate_pair(row) - self._seen.add((row[self._sample_col], row[self._first_col])) - self.modified.append(row) - - def _validate_sample(self, row): - """Assert that the sample name exists and convert spaces to underscores.""" - if len(row[self._sample_col]) <= 0: - raise AssertionError("Sample input is required.") - # Sanitize samples slightly. - row[self._sample_col] = row[self._sample_col].replace(" ", "_") - - def _validate_first(self, row): - """Assert that the first FASTQ entry is non-empty and has the right format.""" - if len(row[self._first_col]) <= 0: - raise AssertionError("At least the first FASTQ file is required.") - self._validate_fastq_format(row[self._first_col]) - - def _validate_second(self, row): - """Assert that the second FASTQ entry has the right format if it exists.""" - if len(row[self._second_col]) > 0: - self._validate_fastq_format(row[self._second_col]) - - def _validate_pair(self, row): - """Assert that read pairs have the same file extension. Report pair status.""" - if row[self._first_col] and row[self._second_col]: - row[self._single_col] = False - first_col_suffix = Path(row[self._first_col]).suffixes[-2:] - second_col_suffix = Path(row[self._second_col]).suffixes[-2:] - if first_col_suffix != second_col_suffix: - raise AssertionError("FASTQ pairs must have the same file extensions.") - else: - row[self._single_col] = True - - def _validate_fastq_format(self, filename): - """Assert that a given filename has one of the expected FASTQ extensions.""" - if not any(filename.endswith(extension) for extension in self.VALID_FORMATS): - raise AssertionError( - f"The FASTQ file has an unrecognized extension: {filename}\n" - f"It should be one of: {', '.join(self.VALID_FORMATS)}" - ) - - def validate_unique_samples(self): - """ - Assert that the combination of sample name and FASTQ filename is unique. - - In addition to the validation, also rename all samples to have a suffix of _T{n}, where n is the - number of times the same sample exist, but with different FASTQ files, e.g., multiple runs per experiment. - - """ - if len(self._seen) != len(self.modified): - raise AssertionError("The pair of sample name and FASTQ must be unique.") - seen = Counter() - for row in self.modified: - sample = row[self._sample_col] - seen[sample] += 1 - row[self._sample_col] = f"{sample}_T{seen[sample]}" - - -def read_head(handle, num_lines=10): - """Read the specified number of lines from the current position in the file.""" - lines = [] - for idx, line in enumerate(handle): - if idx == num_lines: - break - lines.append(line) - return "".join(lines) - - -def sniff_format(handle): - """ - Detect the tabular format. - - Args: - handle (text file): A handle to a `text file`_ object. The read position is - expected to be at the beginning (index 0). - - Returns: - csv.Dialect: The detected tabular format. - - .. _text file: - https://docs.python.org/3/glossary.html#term-text-file - - """ - peek = read_head(handle) - handle.seek(0) - sniffer = csv.Sniffer() - dialect = sniffer.sniff(peek) - return dialect - - -def check_samplesheet(file_in, file_out): - """ - Check that the tabular samplesheet has the structure expected by nf-core pipelines. - - Validate the general shape of the table, expected columns, and each row. Also add - an additional column which records whether one or two FASTQ reads were found. - - Args: - file_in (pathlib.Path): The given tabular samplesheet. The format can be either - CSV, TSV, or any other format automatically recognized by ``csv.Sniffer``. - file_out (pathlib.Path): Where the validated and transformed samplesheet should - be created; always in CSV format. - - Example: - This function checks that the samplesheet follows the following structure, - see also the `viral recon samplesheet`_:: - - sample,fastq_1,fastq_2 - SAMPLE_PE,SAMPLE_PE_RUN1_1.fastq.gz,SAMPLE_PE_RUN1_2.fastq.gz - SAMPLE_PE,SAMPLE_PE_RUN2_1.fastq.gz,SAMPLE_PE_RUN2_2.fastq.gz - SAMPLE_SE,SAMPLE_SE_RUN1_1.fastq.gz, - - .. _viral recon samplesheet: - https://raw.githubusercontent.com/nf-core/test-datasets/viralrecon/samplesheet/samplesheet_test_illumina_amplicon.csv - - """ - required_columns = {"sample", "fastq_1", "fastq_2"} - # See https://docs.python.org/3.9/library/csv.html#id3 to read up on `newline=""`. - with file_in.open(newline="") as in_handle: - reader = csv.DictReader(in_handle, dialect=sniff_format(in_handle)) - # Validate the existence of the expected header columns. - if not required_columns.issubset(reader.fieldnames): - req_cols = ", ".join(required_columns) - logger.critical(f"The sample sheet **must** contain these column headers: {req_cols}.") - sys.exit(1) - # Validate each row. - checker = RowChecker() - for i, row in enumerate(reader): - try: - checker.validate_and_transform(row) - except AssertionError as error: - logger.critical(f"{str(error)} On line {i + 2}.") - sys.exit(1) - checker.validate_unique_samples() - header = list(reader.fieldnames) - header.insert(1, "single_end") - # See https://docs.python.org/3.9/library/csv.html#id3 to read up on `newline=""`. - with file_out.open(mode="w", newline="") as out_handle: - writer = csv.DictWriter(out_handle, header, delimiter=",") - writer.writeheader() - for row in checker.modified: - writer.writerow(row) - - -def parse_args(argv=None): - """Define and immediately parse command line arguments.""" - parser = argparse.ArgumentParser( - description="Validate and transform a tabular samplesheet.", - epilog="Example: python check_samplesheet.py samplesheet.csv samplesheet.valid.csv", - ) - parser.add_argument( - "file_in", - metavar="FILE_IN", - type=Path, - help="Tabular input samplesheet in CSV or TSV format.", - ) - parser.add_argument( - "file_out", - metavar="FILE_OUT", - type=Path, - help="Transformed output samplesheet in CSV format.", - ) - parser.add_argument( - "-l", - "--log-level", - help="The desired log level (default WARNING).", - choices=("CRITICAL", "ERROR", "WARNING", "INFO", "DEBUG"), - default="WARNING", - ) - return parser.parse_args(argv) - - -def main(argv=None): - """Coordinate argument parsing and program execution.""" - args = parse_args(argv) - logging.basicConfig(level=args.log_level, format="[%(levelname)s] %(message)s") - if not args.file_in.is_file(): - logger.error(f"The given input file {args.file_in} was not found!") - sys.exit(2) - args.file_out.parent.mkdir(parents=True, exist_ok=True) - check_samplesheet(args.file_in, args.file_out) - - -if __name__ == "__main__": - sys.exit(main()) diff --git a/lib/WorkflowCmggExomecnv.groovy b/lib/WorkflowCmggExomecnv.groovy index 23c36ca..f329457 100755 --- a/lib/WorkflowCmggExomecnv.groovy +++ b/lib/WorkflowCmggExomecnv.groovy @@ -15,9 +15,9 @@ class WorkflowCmggExomecnv { genomeExistsError(params, log) - if (!params.fasta) { - Nextflow.error "Genome fasta file not specified with e.g. '--fasta genome.fa' or via a detectable config file." - } + // if (!params.fasta) { + // Nextflow.error "Genome fasta file not specified with e.g. '--fasta genome.fa' or via a detectable config file." + // } } // diff --git a/main.nf b/main.nf index 3b699df..9c00410 100644 --- a/main.nf +++ b/main.nf @@ -18,7 +18,7 @@ nextflow.enable.dsl = 2 // TODO nf-core: Remove this line if you don't need a FASTA file // This is an example of how to use getGenomeAttribute() to fetch parameters // from igenomes.config using `--genome` -params.fasta = WorkflowMain.getGenomeAttribute(params, 'fasta') +// params.fasta = WorkflowMain.getGenomeAttribute(params, 'fasta') /* ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ diff --git a/modules.json b/modules.json index ce86deb..d25326a 100644 --- a/modules.json +++ b/modules.json @@ -8,20 +8,26 @@ "custom/dumpsoftwareversions": { "branch": "master", "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", - "installed_by": ["modules"] + "installed_by": [ + "modules" + ] }, - "fastqc": { + "multiqc": { "branch": "master", "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", - "installed_by": ["modules"] + "installed_by": [ + "modules" + ] }, - "multiqc": { + "samtools/convert": { "branch": "master", - "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", - "installed_by": ["modules"] + "git_sha": "a64788f5ad388f1d2ac5bd5f1f3f8fc81476148c", + "installed_by": [ + "modules" + ] } } } } } -} +} \ No newline at end of file diff --git a/modules/nf-core/fastqc/main.nf b/modules/nf-core/fastqc/main.nf deleted file mode 100644 index 50e59f2..0000000 --- a/modules/nf-core/fastqc/main.nf +++ /dev/null @@ -1,55 +0,0 @@ -process FASTQC { - tag "$meta.id" - label 'process_medium' - - conda "${moduleDir}/environment.yml" - container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/fastqc:0.12.1--hdfd78af_0' : - 'biocontainers/fastqc:0.12.1--hdfd78af_0' }" - - input: - tuple val(meta), path(reads) - - output: - tuple val(meta), path("*.html"), emit: html - tuple val(meta), path("*.zip") , emit: zip - path "versions.yml" , emit: versions - - when: - task.ext.when == null || task.ext.when - - script: - def args = task.ext.args ?: '' - def prefix = task.ext.prefix ?: "${meta.id}" - // Make list of old name and new name pairs to use for renaming in the bash while loop - def old_new_pairs = reads instanceof Path || reads.size() == 1 ? [[ reads, "${prefix}.${reads.extension}" ]] : reads.withIndex().collect { entry, index -> [ entry, "${prefix}_${index + 1}.${entry.extension}" ] } - def rename_to = old_new_pairs*.join(' ').join(' ') - def renamed_files = old_new_pairs.collect{ old_name, new_name -> new_name }.join(' ') - """ - printf "%s %s\\n" $rename_to | while read old_name new_name; do - [ -f "\${new_name}" ] || ln -s \$old_name \$new_name - done - - fastqc \\ - $args \\ - --threads $task.cpus \\ - $renamed_files - - cat <<-END_VERSIONS > versions.yml - "${task.process}": - fastqc: \$( fastqc --version | sed -e "s/FastQC v//g" ) - END_VERSIONS - """ - - stub: - def prefix = task.ext.prefix ?: "${meta.id}" - """ - touch ${prefix}.html - touch ${prefix}.zip - - cat <<-END_VERSIONS > versions.yml - "${task.process}": - fastqc: \$( fastqc --version | sed -e "s/FastQC v//g" ) - END_VERSIONS - """ -} diff --git a/modules/nf-core/fastqc/meta.yml b/modules/nf-core/fastqc/meta.yml deleted file mode 100644 index ee5507e..0000000 --- a/modules/nf-core/fastqc/meta.yml +++ /dev/null @@ -1,57 +0,0 @@ -name: fastqc -description: Run FastQC on sequenced reads -keywords: - - quality control - - qc - - adapters - - fastq -tools: - - fastqc: - description: | - FastQC gives general quality metrics about your reads. - It provides information about the quality score distribution - across your reads, the per base sequence content (%A/C/G/T). - You get information about adapter contamination and other - overrepresented sequences. - homepage: https://www.bioinformatics.babraham.ac.uk/projects/fastqc/ - documentation: https://www.bioinformatics.babraham.ac.uk/projects/fastqc/Help/ - licence: ["GPL-2.0-only"] -input: - - meta: - type: map - description: | - Groovy Map containing sample information - e.g. [ id:'test', single_end:false ] - - reads: - type: file - description: | - List of input FastQ files of size 1 and 2 for single-end and paired-end data, - respectively. -output: - - meta: - type: map - description: | - Groovy Map containing sample information - e.g. [ id:'test', single_end:false ] - - html: - type: file - description: FastQC report - pattern: "*_{fastqc.html}" - - zip: - type: file - description: FastQC report archive - pattern: "*_{fastqc.zip}" - - versions: - type: file - description: File containing software versions - pattern: "versions.yml" -authors: - - "@drpatelh" - - "@grst" - - "@ewels" - - "@FelixKrueger" -maintainers: - - "@drpatelh" - - "@grst" - - "@ewels" - - "@FelixKrueger" diff --git a/modules/nf-core/fastqc/tests/main.nf.test b/modules/nf-core/fastqc/tests/main.nf.test deleted file mode 100644 index 6437a14..0000000 --- a/modules/nf-core/fastqc/tests/main.nf.test +++ /dev/null @@ -1,41 +0,0 @@ -nextflow_process { - - name "Test Process FASTQC" - script "../main.nf" - process "FASTQC" - tag "modules" - tag "modules_nfcore" - tag "fastqc" - - test("Single-Read") { - - when { - params { - outdir = "$outputDir" - } - process { - """ - input[0] = [ - [ id: 'test', single_end:true ], - [ - file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true) - ] - ] - """ - } - } - - then { - assertAll ( - { assert process.success }, - // NOTE The report contains the date inside it, which means that the md5sum is stable per day, but not longer than that. So you can't md5sum it. - // looks like this:
Mon 2 Oct 2023
test.gz
- // https://github.com/nf-core/modules/pull/3903#issuecomment-1743620039 - { assert process.out.html.get(0).get(1) ==~ ".*/test_fastqc.html" }, - { assert path(process.out.html.get(0).get(1)).getText().contains("File typeConventional base calls") }, - { assert snapshot(process.out.versions).match("versions") }, - { assert process.out.zip.get(0).get(1) ==~ ".*/test_fastqc.zip" } - ) - } - } -} diff --git a/modules/nf-core/fastqc/tests/main.nf.test.snap b/modules/nf-core/fastqc/tests/main.nf.test.snap deleted file mode 100644 index 636a32c..0000000 --- a/modules/nf-core/fastqc/tests/main.nf.test.snap +++ /dev/null @@ -1,10 +0,0 @@ -{ - "versions": { - "content": [ - [ - "versions.yml:md5,e1cc25ca8af856014824abd842e93978" - ] - ], - "timestamp": "2023-10-09T23:40:54+0000" - } -} \ No newline at end of file diff --git a/modules/nf-core/fastqc/tests/tags.yml b/modules/nf-core/fastqc/tests/tags.yml deleted file mode 100644 index 7834294..0000000 --- a/modules/nf-core/fastqc/tests/tags.yml +++ /dev/null @@ -1,2 +0,0 @@ -fastqc: - - modules/nf-core/fastqc/** diff --git a/modules/nf-core/fastqc/environment.yml b/modules/nf-core/samtools/convert/environment.yml similarity index 56% rename from modules/nf-core/fastqc/environment.yml rename to modules/nf-core/samtools/convert/environment.yml index 1787b38..e2589d5 100644 --- a/modules/nf-core/fastqc/environment.yml +++ b/modules/nf-core/samtools/convert/environment.yml @@ -1,7 +1,7 @@ -name: fastqc +name: samtools_convert channels: - conda-forge - bioconda - defaults dependencies: - - bioconda::fastqc=0.12.1 + - bioconda::samtools=1.18 diff --git a/modules/nf-core/samtools/convert/main.nf b/modules/nf-core/samtools/convert/main.nf new file mode 100644 index 0000000..fef51ca --- /dev/null +++ b/modules/nf-core/samtools/convert/main.nf @@ -0,0 +1,42 @@ +process SAMTOOLS_CONVERT { + tag "$meta.id" + label 'process_low' + + conda "${moduleDir}/environment.yml" + container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? + 'https://depot.galaxyproject.org/singularity/samtools:1.18--h50ea8bc_1' : + 'biocontainers/samtools:1.18--h50ea8bc_1' }" + + input: + tuple val(meta), path(input), path(index) + tuple val(meta2), path(fasta) + tuple val(meta3), path(fai) + + output: + tuple val(meta), path("*.{cram,bam}"), path("*.{crai,bai}") , emit: alignment_index + path "versions.yml" , emit: versions + + when: + task.ext.when == null || task.ext.when + + script: + def args = task.ext.args ?: '' + def prefix = task.ext.prefix ?: "${meta.id}" + def output_extension = input.getExtension() == "bam" ? "cram" : "bam" + + """ + samtools view \\ + --threads ${task.cpus} \\ + --reference ${fasta} \\ + $args \\ + $input \\ + -o ${prefix}.${output_extension} + + samtools index -@${task.cpus} ${prefix}.${output_extension} + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + samtools: \$(echo \$(samtools --version 2>&1) | sed 's/^.*samtools //; s/Using.*\$//') + END_VERSIONS + """ +} diff --git a/modules/nf-core/samtools/convert/meta.yml b/modules/nf-core/samtools/convert/meta.yml new file mode 100644 index 0000000..f8a1612 --- /dev/null +++ b/modules/nf-core/samtools/convert/meta.yml @@ -0,0 +1,55 @@ +name: samtools_convert +description: convert and then index CRAM -> BAM or BAM -> CRAM file +keywords: + - view + - index + - bam + - cram +tools: + - samtools: + description: | + SAMtools is a set of utilities for interacting with and post-processing + short DNA sequence read alignments in the SAM, BAM and CRAM formats, written by Heng Li. + These files are generated as output by short read aligners like BWA. + homepage: http://www.htslib.org/ + documentation: http://www.htslib.org/doc/samtools.html + doi: 10.1093/bioinformatics/btp352 + licence: ["MIT"] +input: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - input: + type: file + description: BAM/CRAM file + pattern: "*.{bam,cram}" + - index: + type: file + description: BAM/CRAM index file + pattern: "*.{bai,crai}" + - fasta: + type: file + description: Reference file to create the CRAM file + pattern: "*.{fasta,fa}" +output: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - alignment_index: + type: file + description: filtered/converted BAM/CRAM file + index + pattern: "*{.bam/cram,.bai/crai}" + - version: + type: file + description: File containing software version + pattern: "*.{version.txt}" +authors: + - "@FriederikeHanssen" + - "@maxulysse" +maintainers: + - "@FriederikeHanssen" + - "@maxulysse" diff --git a/modules/nf-core/samtools/convert/tests/main.nf.test b/modules/nf-core/samtools/convert/tests/main.nf.test new file mode 100644 index 0000000..7087293 --- /dev/null +++ b/modules/nf-core/samtools/convert/tests/main.nf.test @@ -0,0 +1,82 @@ +nextflow_process { + + name "Test Process SAMTOOLS_CONVERT" + script "../main.nf" + process "SAMTOOLS_CONVERT" + + tag "modules" + tag "modules_nfcore" + tag "samtools" + tag "samtools/convert" + + test("sarscov2 - [bam, bai], fasta, fai") { + + when { + process { + """ + input[0] = [ + [ id:'test', single_end:false ], // meta map + file(params.test_data['sarscov2']['illumina']['test_paired_end_sorted_bam'], checkIfExists: true), + file(params.test_data['sarscov2']['illumina']['test_paired_end_sorted_bam_bai'], checkIfExists: true) + ] + input[1] = [ + [id:'fasta'], + file(params.test_data['sarscov2']['genome']['genome_fasta'], checkIfExists: true) + ] + input[2] = [ + [id:'fai'], + file(params.test_data['sarscov2']['genome']['genome_fasta_fai'], checkIfExists: true) + ] + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot( + file(process.out.alignment_index[0][1]).name, + file(process.out.alignment_index[0][2]).name, + process.out.versions + ).match() } + ) + } + + } + + test("homo_sapiens - [cram, crai], fasta, fai") { + + when { + process { + """ + input[0] = [ + [ id:'test', single_end:false ], // meta map + file(params.test_data['homo_sapiens']['illumina']['test_paired_end_recalibrated_sorted_cram'], checkIfExists: true), + file(params.test_data['homo_sapiens']['illumina']['test_paired_end_recalibrated_sorted_cram_crai'], checkIfExists: true) + ] + input[1] = [ + [id:'fasta'], + file(params.test_data['homo_sapiens']['genome']['genome_fasta'], checkIfExists: true) + ] + input[2] = [ + [id:'fai'], + file(params.test_data['homo_sapiens']['genome']['genome_fasta_fai'], checkIfExists: true) + ] + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot( + file(process.out.alignment_index[0][1]).name, + file(process.out.alignment_index[0][2]).name, + process.out.versions + ).match() } + ) + } + + } + +} diff --git a/modules/nf-core/samtools/convert/tests/main.nf.test.snap b/modules/nf-core/samtools/convert/tests/main.nf.test.snap new file mode 100644 index 0000000..fe32f14 --- /dev/null +++ b/modules/nf-core/samtools/convert/tests/main.nf.test.snap @@ -0,0 +1,22 @@ +{ + "homo_sapiens - [cram, crai], fasta, fai": { + "content": [ + "test.bam", + "test.bam.bai", + [ + "versions.yml:md5,bca738d24a22140d2d5c3ed5060a8896" + ] + ], + "timestamp": "2023-12-04T14:18:33.327409248" + }, + "sarscov2 - [bam, bai], fasta, fai": { + "content": [ + "test.cram", + "test.cram.crai", + [ + "versions.yml:md5,bca738d24a22140d2d5c3ed5060a8896" + ] + ], + "timestamp": "2023-12-04T14:16:15.221347765" + } +} \ No newline at end of file diff --git a/modules/nf-core/samtools/convert/tests/tags.yml b/modules/nf-core/samtools/convert/tests/tags.yml new file mode 100644 index 0000000..030d5eb --- /dev/null +++ b/modules/nf-core/samtools/convert/tests/tags.yml @@ -0,0 +1,2 @@ +samtools/convert: + - "modules/nf-core/samtools/convert/**" diff --git a/nextflow.config b/nextflow.config index a1e9404..c19800e 100644 --- a/nextflow.config +++ b/nextflow.config @@ -12,11 +12,13 @@ params { // TODO nf-core: Specify your pipeline's command line flags // Input options input = null + // References - genome = null - igenomes_base = 's3://ngi-igenomes/igenomes/' - igenomes_ignore = false - + genome = "GRCh38" + cmgg_config_base = "/conf/" + igenomes_base = null //'s3://ngi-igenomes/igenomes/' + igenomes_ignore = true + // MultiQC options multiqc_config = null @@ -43,7 +45,7 @@ params { custom_config_base = "https://raw.githubusercontent.com/nf-core/configs/${params.custom_config_version}" config_profile_contact = null config_profile_url = null - + // Max resource options // Defaults only, expecting to be overwritten diff --git a/nextflow_schema.json b/nextflow_schema.json index 542fb8f..e5a0c17 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -71,6 +71,12 @@ "fa_icon": "fas fa-ban", "hidden": true, "help_text": "Do not load `igenomes.config` when running the pipeline. You may choose this option if you observe clashes between custom parameters and those supplied in `igenomes.config`." + }, + "cmgg_config_base": { + "type": "string", + "default": "/conf/", + "description": "The config base path for the cmgg configs", + "format": "directory-path" } } }, diff --git a/subworkflows/local/bam_variant_calling_exomedepth/main.nf b/subworkflows/local/bam_variant_calling_exomedepth/main.nf new file mode 100644 index 0000000..4757926 --- /dev/null +++ b/subworkflows/local/bam_variant_calling_exomedepth/main.nf @@ -0,0 +1,37 @@ +// TODO nf-core: If in doubt look at other nf-core/subworkflows to see how we are doing things! :) +// https://github.com/nf-core/modules/tree/master/subworkflows +// You can also ask for help via your pull request or on the #subworkflows channel on the nf-core Slack workspace: +// https://nf-co.re/join +// TODO nf-core: A subworkflow SHOULD import at least two modules + +// Convert CRAM files (optional) +include { SAMTOOLS_CONVERT as CRAM_TO_BAM } from '../../../modules/nf-core/samtools/convert/main' +// include { SAMTOOLS_INDEX } from '../../../modules/nf-core/samtools/index/main' + +workflow BAM_VARIANT_CALLING_EXOMEDEPTH { + + take: + // TODO nf-core: edit input (take) channels + ch_bam // channel: [ val(meta), [ bam ] ] + + main: + + ch_versions = Channel.empty() + + // TODO nf-core: substitute modules here for the modules of your subworkflow + + SAMTOOLS_SORT ( ch_bam ) + ch_versions = ch_versions.mix(SAMTOOLS_SORT.out.versions.first()) + + SAMTOOLS_INDEX ( SAMTOOLS_SORT.out.bam ) + ch_versions = ch_versions.mix(SAMTOOLS_INDEX.out.versions.first()) + + emit: + // TODO nf-core: edit emitted channels + bam = SAMTOOLS_SORT.out.bam // channel: [ val(meta), [ bam ] ] + bai = SAMTOOLS_INDEX.out.bai // channel: [ val(meta), [ bai ] ] + csi = SAMTOOLS_INDEX.out.csi // channel: [ val(meta), [ csi ] ] + + versions = ch_versions // channel: [ versions.yml ] +} + diff --git a/workflows/cmggexomecnv.nf b/workflows/cmggexomecnv.nf index 71dc4f2..4b4885f 100644 --- a/workflows/cmggexomecnv.nf +++ b/workflows/cmggexomecnv.nf @@ -35,7 +35,7 @@ ch_multiqc_custom_methods_description = params.multiqc_methods_description ? fil // // SUBWORKFLOW: Consisting of a mix of local and nf-core/modules // - +include { BAM_VARIANT_CALLING_EXOMEDEPTH } from '../subworkflows/local/bam_variant_calling_exomedepth/main' /* ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ IMPORT NF-CORE MODULES/SUBWORKFLOWS @@ -45,10 +45,9 @@ ch_multiqc_custom_methods_description = params.multiqc_methods_description ? fil // // MODULE: Installed directly from nf-core/modules // -include { FASTQC } from '../modules/nf-core/fastqc/main' +// include { FASTQC } from '../modules/nf-core/fastqc/main' include { MULTIQC } from '../modules/nf-core/multiqc/main' include { CUSTOM_DUMPSOFTWAREVERSIONS } from '../modules/nf-core/custom/dumpsoftwareversions/main' - /* ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ RUN MAIN WORKFLOW @@ -66,14 +65,15 @@ workflow CMGGEXOMECNV { // SUBWORKFLOW: Read in samplesheet, validate and stage input files // ch_input = Channel.fromSamplesheet("input") + ch_input.view() // // MODULE: Run FastQC // - FASTQC ( - ch_input - ) - ch_versions = ch_versions.mix(FASTQC.out.versions.first()) + // FASTQC ( + // ch_input + // ) + // ch_versions = ch_versions.mix(FASTQC.out.versions.first()) CUSTOM_DUMPSOFTWAREVERSIONS ( ch_versions.unique().collectFile(name: 'collated_versions.yml') @@ -92,7 +92,7 @@ workflow CMGGEXOMECNV { ch_multiqc_files = ch_multiqc_files.mix(ch_workflow_summary.collectFile(name: 'workflow_summary_mqc.yaml')) ch_multiqc_files = ch_multiqc_files.mix(ch_methods_description.collectFile(name: 'methods_description_mqc.yaml')) ch_multiqc_files = ch_multiqc_files.mix(CUSTOM_DUMPSOFTWAREVERSIONS.out.mqc_yml.collect()) - ch_multiqc_files = ch_multiqc_files.mix(FASTQC.out.zip.collect{it[1]}.ifEmpty([])) + // ch_multiqc_files = ch_multiqc_files.mix(FASTQC.out.zip.collect{it[1]}.ifEmpty([])) MULTIQC ( ch_multiqc_files.collect(), From b686438a33d028e4f0722ad20ac95ead9ff8d7eb Mon Sep 17 00:00:00 2001 From: torossee Date: Mon, 4 Mar 2024 16:22:27 +0100 Subject: [PATCH 04/64] rename repo name --- .github/CONTRIBUTING.md | 16 ++++++------ .github/ISSUE_TEMPLATE/bug_report.yml | 2 +- .github/ISSUE_TEMPLATE/feature_request.yml | 2 +- .github/PULL_REQUEST_TEMPLATE.md | 8 +++--- .github/workflows/branch.yml | 4 +-- .github/workflows/ci.yml | 2 +- .github/workflows/fix-linting.yml | 2 +- CHANGELOG.md | 4 +-- CITATIONS.md | 2 +- README.md | 29 +++++++++++----------- assets/adaptivecard.json | 2 +- assets/email_template.html | 14 +++++------ assets/email_template.txt | 8 +++--- assets/methods_description_template.yml | 6 ++--- assets/multiqc_config.yml | 6 ++--- assets/schema_input.json | 4 +-- assets/slackreport.json | 2 +- conf/base.config | 2 +- conf/test.config | 2 +- conf/test_full.config | 2 +- docs/README.md | 4 +-- docs/output.md | 2 +- docs/usage.md | 10 ++++---- lib/WorkflowCmggExomecnv.groovy | 2 +- lib/WorkflowMain.groovy | 2 +- main.nf | 6 ++--- modules.json | 4 +-- modules/local/samplesheet_check.nf | 2 +- nextflow.config | 8 +++--- nextflow_schema.json | 4 +-- 30 files changed, 81 insertions(+), 82 deletions(-) diff --git a/.github/CONTRIBUTING.md b/.github/CONTRIBUTING.md index d4ecde9..5a1af1c 100644 --- a/.github/CONTRIBUTING.md +++ b/.github/CONTRIBUTING.md @@ -1,9 +1,9 @@ -# CenterForMedicalGeneticsGhent/nf-cmgg-exomecnv: Contributing Guidelines +# nf-cmgg/exomecnv: Contributing Guidelines Hi there! -Many thanks for taking an interest in improving CenterForMedicalGeneticsGhent/nf-cmgg-exomecnv. +Many thanks for taking an interest in improving nf-cmgg/exomecnv. -We try to manage the required tasks for CenterForMedicalGeneticsGhent/nf-cmgg-exomecnv using GitHub issues, you probably came to this page when creating one. +We try to manage the required tasks for nf-cmgg/exomecnv using GitHub issues, you probably came to this page when creating one. Please use the pre-filled template to save time. However, don't be put off by this template - other more general issues and suggestions are welcome! @@ -11,10 +11,10 @@ Contributions to the code are even more welcome ;) ## Contribution workflow -If you'd like to write some code for CenterForMedicalGeneticsGhent/nf-cmgg-exomecnv, the standard workflow is as follows: +If you'd like to write some code for nf-cmgg/exomecnv, the standard workflow is as follows: -1. Check that there isn't already an issue about your idea in the [CenterForMedicalGeneticsGhent/nf-cmgg-exomecnv issues](https://github.com/CenterForMedicalGeneticsGhent/nf-cmgg-exomecnv/issues) to avoid duplicating work. If there isn't one already, please create one so that others know you're working on this -2. [Fork](https://help.github.com/en/github/getting-started-with-github/fork-a-repo) the [CenterForMedicalGeneticsGhent/nf-cmgg-exomecnv repository](https://github.com/CenterForMedicalGeneticsGhent/nf-cmgg-exomecnv) to your GitHub account +1. Check that there isn't already an issue about your idea in the [nf-cmgg/exomecnv issues](https://github.com/nf-cmgg/exomecnv/issues) to avoid duplicating work. If there isn't one already, please create one so that others know you're working on this +2. [Fork](https://help.github.com/en/github/getting-started-with-github/fork-a-repo) the [nf-cmgg/exomecnv repository](https://github.com/nf-cmgg/exomecnv) to your GitHub account 3. Make the necessary changes / additions within your forked repository following [Pipeline conventions](#pipeline-contribution-conventions) 4. Use `nf-core schema build` and add any new parameters to the pipeline JSON schema (requires [nf-core tools](https://github.com/nf-core/tools) >= 1.10). 5. Submit a Pull Request against the `dev` branch and wait for the code to be reviewed and merged @@ -55,7 +55,7 @@ These tests are run both with the latest available version of `Nextflow` and als ## Pipeline contribution conventions -To make the CenterForMedicalGeneticsGhent/nf-cmgg-exomecnv code and processing logic more understandable for new contributors and to ensure quality, we semi-standardise the way the code and other contributions are written. +To make the nf-cmgg/exomecnv code and processing logic more understandable for new contributors and to ensure quality, we semi-standardise the way the code and other contributions are written. ### Adding a new step @@ -105,7 +105,7 @@ This repo includes a devcontainer configuration which will create a GitHub Codes To get started: -- Open the repo in [Codespaces](https://github.com/CenterForMedicalGeneticsGhent/nf-cmgg-exomecnv/codespaces) +- Open the repo in [Codespaces](https://github.com/nf-cmgg/exomecnv/codespaces) - Tools installed - nf-core - Nextflow diff --git a/.github/ISSUE_TEMPLATE/bug_report.yml b/.github/ISSUE_TEMPLATE/bug_report.yml index 487b21c..450ccd2 100644 --- a/.github/ISSUE_TEMPLATE/bug_report.yml +++ b/.github/ISSUE_TEMPLATE/bug_report.yml @@ -49,7 +49,7 @@ body: * OS _(eg. CentOS Linux, macOS, Linux Mint)_ - * Version of CenterForMedicalGeneticsGhent/nf-cmgg-exomecnv _(eg. 1.1, 1.5, + * Version of nf-cmgg/exomecnv _(eg. 1.1, 1.5, 1.8.2)_ ' diff --git a/.github/ISSUE_TEMPLATE/feature_request.yml b/.github/ISSUE_TEMPLATE/feature_request.yml index f2536f6..e88bf0b 100644 --- a/.github/ISSUE_TEMPLATE/feature_request.yml +++ b/.github/ISSUE_TEMPLATE/feature_request.yml @@ -1,5 +1,5 @@ name: Feature request -description: Suggest an idea for the CenterForMedicalGeneticsGhent/nf-cmgg-exomecnv pipeline +description: Suggest an idea for the nf-cmgg/exomecnv pipeline labels: enhancement body: - type: textarea diff --git a/.github/PULL_REQUEST_TEMPLATE.md b/.github/PULL_REQUEST_TEMPLATE.md index cb3c5b6..a1c8add 100644 --- a/.github/PULL_REQUEST_TEMPLATE.md +++ b/.github/PULL_REQUEST_TEMPLATE.md @@ -1,21 +1,21 @@ ## PR checklist - [ ] This comment contains a description of changes (with reason). - [ ] If you've fixed a bug or added code that should be tested, add tests! -- [ ] If you've added a new tool - have you followed the pipeline conventions in the [contribution docs](https://github.com/CenterForMedicalGeneticsGhent/nf-cmgg-exomecnv/tree/master/.github/CONTRIBUTING.md) +- [ ] If you've added a new tool - have you followed the pipeline conventions in the [contribution docs](https://github.com/nf-cmgg/exomecnv/tree/master/.github/CONTRIBUTING.md) - [ ] Make sure your code lints (`nf-core lint`). - [ ] Ensure the test suite passes (`nextflow run . -profile test,docker --outdir `). - [ ] Check for unexpected warnings in debug mode (`nextflow run . -profile debug,test,docker --outdir `). diff --git a/.github/workflows/branch.yml b/.github/workflows/branch.yml index 5637b58..723cd1f 100644 --- a/.github/workflows/branch.yml +++ b/.github/workflows/branch.yml @@ -11,9 +11,9 @@ jobs: steps: # PRs to the nf-core repo master branch are only ok if coming from the nf-core repo `dev` or any `patch` branches - name: Check PRs - if: github.repository == 'CenterForMedicalGeneticsGhent/nf-cmgg-exomecnv' + if: github.repository == 'nf-cmgg/exomecnv' run: | - { [[ ${{github.event.pull_request.head.repo.full_name }} == CenterForMedicalGeneticsGhent/nf-cmgg-exomecnv ]] && [[ $GITHUB_HEAD_REF == "dev" ]]; } || [[ $GITHUB_HEAD_REF == "patch" ]] + { [[ ${{github.event.pull_request.head.repo.full_name }} == nf-cmgg/exomecnv ]] && [[ $GITHUB_HEAD_REF == "dev" ]]; } || [[ $GITHUB_HEAD_REF == "patch" ]] # If the above check failed, post a comment on the PR explaining the failure # NOTE - this doesn't currently work if the PR is coming from a fork, due to limitations in GitHub actions secrets diff --git a/.github/workflows/ci.yml b/.github/workflows/ci.yml index 0e9155a..7547cd4 100644 --- a/.github/workflows/ci.yml +++ b/.github/workflows/ci.yml @@ -19,7 +19,7 @@ jobs: test: name: Run pipeline with test data # Only run on push if this is the nf-core dev branch (merged PRs) - if: "${{ github.event_name != 'push' || (github.event_name == 'push' && github.repository == 'CenterForMedicalGeneticsGhent/nf-cmgg-exomecnv') }}" + if: "${{ github.event_name != 'push' || (github.event_name == 'push' && github.repository == 'nf-cmgg/exomecnv') }}" runs-on: ubuntu-latest strategy: matrix: diff --git a/.github/workflows/fix-linting.yml b/.github/workflows/fix-linting.yml index 3543ad2..02a549a 100644 --- a/.github/workflows/fix-linting.yml +++ b/.github/workflows/fix-linting.yml @@ -9,7 +9,7 @@ jobs: if: > contains(github.event.comment.html_url, '/pull/') && contains(github.event.comment.body, '@nf-core-bot fix linting') && - github.repository == 'CenterForMedicalGeneticsGhent/nf-cmgg-exomecnv' + github.repository == 'nf-cmgg/exomecnv' runs-on: ubuntu-latest steps: # Use the @nf-core-bot token to check out so we can push later diff --git a/CHANGELOG.md b/CHANGELOG.md index 4291585..8b4d42a 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -1,11 +1,11 @@ -# CenterForMedicalGeneticsGhent/nf-cmgg-exomecnv: Changelog +# nf-cmgg/exomecnv: Changelog The format is based on [Keep a Changelog](https://keepachangelog.com/en/1.0.0/) and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0.html). ## v1.0dev - [date] -Initial release of CenterForMedicalGeneticsGhent/nf-cmgg-exomecnv, created with the [nf-core](https://nf-co.re/) template. +Initial release of nf-cmgg/exomecnv, created with the [nf-core](https://nf-co.re/) template. ### `Added` diff --git a/CITATIONS.md b/CITATIONS.md index 70fc7d8..b2570fc 100644 --- a/CITATIONS.md +++ b/CITATIONS.md @@ -1,4 +1,4 @@ -# CenterForMedicalGeneticsGhent/nf-cmgg-exomecnv: Citations +# nf-cmgg/exomecnv: Citations ## [nf-core](https://pubmed.ncbi.nlm.nih.gov/32055031/) diff --git a/README.md b/README.md index 68efb06..f1367dd 100644 --- a/README.md +++ b/README.md @@ -1,15 +1,15 @@ -[![GitHub Actions CI Status](https://github.com/CenterForMedicalGeneticsGhent/nf-cmgg-exomecnv/workflows/nf-core%20CI/badge.svg)](https://github.com/CenterForMedicalGeneticsGhent/nf-cmgg-exomecnv/actions?query=workflow%3A%22nf-core+CI%22) -[![GitHub Actions Linting Status](https://github.com/CenterForMedicalGeneticsGhent/nf-cmgg-exomecnv/workflows/nf-core%20linting/badge.svg)](https://github.com/CenterForMedicalGeneticsGhent/nf-cmgg-exomecnv/actions?query=workflow%3A%22nf-core+linting%22)[![Cite with Zenodo](http://img.shields.io/badge/DOI-10.5281/zenodo.XXXXXXX-1073c8?labelColor=000000)](https://doi.org/10.5281/zenodo.XXXXXXX) +[![GitHub Actions CI Status](https://github.com/nf-cmgg/exomecnv/workflows/nf-core%20CI/badge.svg)](https://github.com/nf-cmgg/exomecnv/actions?query=workflow%3A%22nf-core+CI%22) +[![GitHub Actions Linting Status](https://github.com/nf-cmgg/exomecnv/workflows/nf-core%20linting/badge.svg)](https://github.com/nf-cmgg/exomecnv/actions?query=workflow%3A%22nf-core+linting%22)[![Cite with Zenodo](http://img.shields.io/badge/DOI-10.5281/zenodo.XXXXXXX-1073c8?labelColor=000000)](https://doi.org/10.5281/zenodo.XXXXXXX) [![Nextflow](https://img.shields.io/badge/nextflow%20DSL2-%E2%89%A523.04.0-23aa62.svg)](https://www.nextflow.io/) [![run with conda](http://img.shields.io/badge/run%20with-conda-3EB049?labelColor=000000&logo=anaconda)](https://docs.conda.io/en/latest/) [![run with docker](https://img.shields.io/badge/run%20with-docker-0db7ed?labelColor=000000&logo=docker)](https://www.docker.com/) [![run with singularity](https://img.shields.io/badge/run%20with-singularity-1d355c.svg?labelColor=000000)](https://sylabs.io/docs/) -[![Launch on Nextflow Tower](https://img.shields.io/badge/Launch%20%F0%9F%9A%80-Nextflow%20Tower-%234256e7)](https://tower.nf/launch?pipeline=https://github.com/CenterForMedicalGeneticsGhent/nf-cmgg-exomecnv) +[![Launch on Nextflow Tower](https://img.shields.io/badge/Launch%20%F0%9F%9A%80-Nextflow%20Tower-%234256e7)](https://tower.nf/launch?pipeline=https://github.com/nf-cmgg/exomecnv) ## Introduction -**CenterForMedicalGeneticsGhent/nf-cmgg-exomecnv** is a bioinformatics pipeline that ... +**nf-cmgg/exomecnv** is a bioinformatics pipeline that ... ```bash -nextflow run CenterForMedicalGeneticsGhent/nf-cmgg-exomecnv \ +nextflow run nf-cmgg/exomecnv \ -profile \ --input samplesheet.csv \ --outdir @@ -75,7 +74,7 @@ see [docs](https://nf-co.re/usage/configuration#custom-configuration-files). ## Credits -CenterForMedicalGeneticsGhent/nf-cmgg-exomecnv was originally written by nvnieuwk. +nf-cmgg/exomecnv was originally written by nvnieuwk. We thank the following people for their extensive assistance in the development of this pipeline: @@ -88,7 +87,7 @@ If you would like to contribute to this pipeline, please see the [contributing g ## Citations - + diff --git a/assets/adaptivecard.json b/assets/adaptivecard.json index f7dc263..24298f2 100644 --- a/assets/adaptivecard.json +++ b/assets/adaptivecard.json @@ -17,7 +17,7 @@ "size": "Large", "weight": "Bolder", "color": "<% if (success) { %>Good<% } else { %>Attention<%} %>", - "text": "CenterForMedicalGeneticsGhent/nf-cmgg-exomecnv v${version} - ${runName}", + "text": "nf-cmgg/exomecnv v${version} - ${runName}", "wrap": true }, { diff --git a/assets/email_template.html b/assets/email_template.html index aa7ac63..246ec9c 100644 --- a/assets/email_template.html +++ b/assets/email_template.html @@ -4,21 +4,21 @@ - - CenterForMedicalGeneticsGhent/nf-cmgg-exomecnv Pipeline Report + + nf-cmgg/exomecnv Pipeline Report
-

CenterForMedicalGeneticsGhent/nf-cmgg-exomecnv v${version}

+

nf-cmgg/exomecnv v${version}

Run Name: $runName

<% if (!success){ out << """
-

CenterForMedicalGeneticsGhent/nf-cmgg-exomecnv execution completed unsuccessfully!

+

nf-cmgg/exomecnv execution completed unsuccessfully!

The exit status of the task that caused the workflow execution to fail was: $exitStatus.

The full error message was:

${errorReport}
@@ -27,7 +27,7 @@

CenterForMedicalGeneticsGhent/nf-cmgg- } else { out << """
- CenterForMedicalGeneticsGhent/nf-cmgg-exomecnv execution completed successfully! + nf-cmgg/exomecnv execution completed successfully!
""" } @@ -44,8 +44,8 @@

Pipeline Configuration:

-

CenterForMedicalGeneticsGhent/nf-cmgg-exomecnv

-

https://github.com/CenterForMedicalGeneticsGhent/nf-cmgg-exomecnv

+

nf-cmgg/exomecnv

+

https://github.com/nf-cmgg/exomecnv

diff --git a/assets/email_template.txt b/assets/email_template.txt index 247ba11..92d7395 100644 --- a/assets/email_template.txt +++ b/assets/email_template.txt @@ -1,10 +1,10 @@ Run Name: $runName <% if (success){ - out << "## CenterForMedicalGeneticsGhent/nf-cmgg-exomecnv execution completed successfully! ##" + out << "## nf-cmgg/exomecnv execution completed successfully! ##" } else { out << """#################################################### -## CenterForMedicalGeneticsGhent/nf-cmgg-exomecnv execution completed unsuccessfully! ## +## nf-cmgg/exomecnv execution completed unsuccessfully! ## #################################################### The exit status of the task that caused the workflow execution to fail was: $exitStatus. The full error message was: @@ -27,5 +27,5 @@ Pipeline Configuration: <% out << summary.collect{ k,v -> " - $k: $v" }.join("\n") %> -- -CenterForMedicalGeneticsGhent/nf-cmgg-exomecnv -https://github.com/CenterForMedicalGeneticsGhent/nf-cmgg-exomecnv +nf-cmgg/exomecnv +https://github.com/nf-cmgg/exomecnv diff --git a/assets/methods_description_template.yml b/assets/methods_description_template.yml index 79802a0..7bac535 100644 --- a/assets/methods_description_template.yml +++ b/assets/methods_description_template.yml @@ -1,13 +1,13 @@ id: "CenterForMedicalGeneticsGhent-nf-cmgg-exomecnv-methods-description" description: "Suggested text and references to use when describing pipeline usage within the methods section of a publication." -section_name: "CenterForMedicalGeneticsGhent/nf-cmgg-exomecnv Methods Description" -section_href: "https://github.com/CenterForMedicalGeneticsGhent/nf-cmgg-exomecnv" +section_name: "nf-cmgg/exomecnv Methods Description" +section_href: "https://github.com/nf-cmgg/exomecnv" plot_type: "html" ## TODO nf-core: Update the HTML below to your preferred methods description, e.g. add publication citation for this pipeline ## You inject any metadata in the Nextflow '${workflow}' object data: |

Methods

-

Data was processed using CenterForMedicalGeneticsGhent/nf-cmgg-exomecnv v${workflow.manifest.version} ${doi_text} of the nf-core collection of workflows (Ewels et al., 2020), utilising reproducible software environments from the Bioconda (Grüning et al., 2018) and Biocontainers (da Veiga Leprevost et al., 2017) projects.

+

Data was processed using nf-cmgg/exomecnv v${workflow.manifest.version} ${doi_text} of the nf-core collection of workflows (Ewels et al., 2020), utilising reproducible software environments from the Bioconda (Grüning et al., 2018) and Biocontainers (da Veiga Leprevost et al., 2017) projects.

The pipeline was executed with Nextflow v${workflow.nextflow.version} (Di Tommaso et al., 2017) with the following command:

${workflow.commandLine}

${tool_citations}

diff --git a/assets/multiqc_config.yml b/assets/multiqc_config.yml index 287b84b..ae2ead0 100644 --- a/assets/multiqc_config.yml +++ b/assets/multiqc_config.yml @@ -1,12 +1,12 @@ report_comment: > - This report has been generated by the CenterForMedicalGeneticsGhent/nf-cmgg-exomecnv + This report has been generated by the nf-cmgg/exomecnv analysis pipeline. report_section_order: - "CenterForMedicalGeneticsGhent-nf-cmgg-exomecnv-methods-description": + "nf-cmgg-exomecnv-methods-description": order: -1000 software_versions: order: -1001 - "CenterForMedicalGeneticsGhent-nf-cmgg-exomecnv-summary": + "nf-cmgg-exomecnv-summary": order: -1002 export_plots: true diff --git a/assets/schema_input.json b/assets/schema_input.json index cc71c7a..86775a3 100644 --- a/assets/schema_input.json +++ b/assets/schema_input.json @@ -1,7 +1,7 @@ { "$schema": "http://json-schema.org/draft-07/schema", - "$id": "https://raw.githubusercontent.com/CenterForMedicalGeneticsGhent/nf-cmgg-exomecnv/master/assets/schema_input.json", - "title": "CenterForMedicalGeneticsGhent/nf-cmgg-exomecnv pipeline - params.input schema", + "$id": "https://raw.githubusercontent.com/nf-cmgg/exomecnv/master/assets/schema_input.json", + "title": "nf-cmgg/exomecnv pipeline - params.input schema", "description": "Schema for the file provided with params.input", "type": "array", "items": { diff --git a/assets/slackreport.json b/assets/slackreport.json index dd93025..84aba38 100644 --- a/assets/slackreport.json +++ b/assets/slackreport.json @@ -3,7 +3,7 @@ { "fallback": "Plain-text summary of the attachment.", "color": "<% if (success) { %>good<% } else { %>danger<%} %>", - "author_name": "CenterForMedicalGeneticsGhent/nf-cmgg-exomecnv ${version} - ${runName}", + "author_name": "nf-cmgg/exomecnv ${version} - ${runName}", "author_icon": "https://www.nextflow.io/docs/latest/_static/favicon.ico", "text": "<% if (success) { %>Pipeline completed successfully!<% } else { %>Pipeline completed with errors<% } %>", "fields": [ diff --git a/conf/base.config b/conf/base.config index 37f670d..bbae968 100644 --- a/conf/base.config +++ b/conf/base.config @@ -1,6 +1,6 @@ /* ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ - CenterForMedicalGeneticsGhent/nf-cmgg-exomecnv Nextflow base config file + nf-cmgg/exomecnv Nextflow base config file ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ A 'blank slate' config file, appropriate for general use on most high performance compute environments. Assumes that all software is installed and available on diff --git a/conf/test.config b/conf/test.config index f1a6b13..893b94c 100644 --- a/conf/test.config +++ b/conf/test.config @@ -5,7 +5,7 @@ Defines input files and everything required to run a fast and simple pipeline test. Use as follows: - nextflow run CenterForMedicalGeneticsGhent/nf-cmgg-exomecnv -profile test, --outdir + nextflow run nf-cmgg/exomecnv -profile test, --outdir ---------------------------------------------------------------------------------------- */ diff --git a/conf/test_full.config b/conf/test_full.config index eb86cdc..46990f4 100644 --- a/conf/test_full.config +++ b/conf/test_full.config @@ -5,7 +5,7 @@ Defines input files and everything required to run a full size pipeline test. Use as follows: - nextflow run CenterForMedicalGeneticsGhent/nf-cmgg-exomecnv -profile test_full, --outdir + nextflow run nf-cmgg/exomecnv -profile test_full, --outdir ---------------------------------------------------------------------------------------- */ diff --git a/docs/README.md b/docs/README.md index d02d01b..2b19b49 100644 --- a/docs/README.md +++ b/docs/README.md @@ -1,6 +1,6 @@ -# CenterForMedicalGeneticsGhent/nf-cmgg-exomecnv: Documentation +# nf-cmgg/exomecnv: Documentation -The CenterForMedicalGeneticsGhent/nf-cmgg-exomecnv documentation is split into the following pages: +The nf-cmgg/exomecnv documentation is split into the following pages: - [Usage](usage.md) - An overview of how the pipeline works, how to run it and a description of all of the different command-line flags. diff --git a/docs/output.md b/docs/output.md index c1f10be..1fa4efd 100644 --- a/docs/output.md +++ b/docs/output.md @@ -1,4 +1,4 @@ -# CenterForMedicalGeneticsGhent/nf-cmgg-exomecnv: Output +# nf-cmgg/exomecnv: Output ## Introduction diff --git a/docs/usage.md b/docs/usage.md index 70cc949..3be8450 100644 --- a/docs/usage.md +++ b/docs/usage.md @@ -1,4 +1,4 @@ -# CenterForMedicalGeneticsGhent/nf-cmgg-exomecnv: Usage +# nf-cmgg/exomecnv: Usage > _Documentation of pipeline parameters is generated automatically from the pipeline schema and can no longer be found in markdown files._ @@ -55,7 +55,7 @@ An [example samplesheet](../assets/samplesheet.csv) has been provided with the p The typical command for running the pipeline is as follows: ```bash -nextflow run CenterForMedicalGeneticsGhent/nf-cmgg-exomecnv --input ./samplesheet.csv --outdir ./results --genome GRCh37 -profile docker +nextflow run nf-cmgg/exomecnv --input ./samplesheet.csv --outdir ./results --genome GRCh37 -profile docker ``` This will launch the pipeline with the `docker` configuration profile. See below for more information about profiles. @@ -80,7 +80,7 @@ Do not use `-c ` to specify parameters as this will result in errors. Cust The above pipeline run specified with a params file in yaml format: ```bash -nextflow run CenterForMedicalGeneticsGhent/nf-cmgg-exomecnv -profile docker -params-file params.yaml +nextflow run nf-cmgg/exomecnv -profile docker -params-file params.yaml ``` with `params.yaml` containing: @@ -99,14 +99,14 @@ You can also generate such `YAML`/`JSON` files via [nf-core/launch](https://nf-c When you run the above command, Nextflow automatically pulls the pipeline code from GitHub and stores it as a cached version. When running the pipeline after this, it will always use the cached version if available - even if the pipeline has been updated since. To make sure that you're running the latest version of the pipeline, make sure that you regularly update the cached version of the pipeline: ```bash -nextflow pull CenterForMedicalGeneticsGhent/nf-cmgg-exomecnv +nextflow pull nf-cmgg/exomecnv ``` ### Reproducibility It is a good idea to specify a pipeline version when running the pipeline on your data. This ensures that a specific version of the pipeline code and software are used when you run your pipeline. If you keep using the same tag, you'll be running the same version of the pipeline, even if there have been changes to the code since. -First, go to the [CenterForMedicalGeneticsGhent/nf-cmgg-exomecnv releases page](https://github.com/CenterForMedicalGeneticsGhent/nf-cmgg-exomecnv/releases) and find the latest pipeline version - numeric only (eg. `1.3.1`). Then specify this when running the pipeline with `-r` (one hyphen) - eg. `-r 1.3.1`. Of course, you can switch to another version by changing the number after the `-r` flag. +First, go to the [nf-cmgg/exomecnv releases page](https://github.com/nf-cmgg/exomecnv/releases) and find the latest pipeline version - numeric only (eg. `1.3.1`). Then specify this when running the pipeline with `-r` (one hyphen) - eg. `-r 1.3.1`. Of course, you can switch to another version by changing the number after the `-r` flag. This version number will be logged in reports when you run the pipeline, so that you'll know what you used when you look back in the future. For example, at the bottom of the MultiQC reports. diff --git a/lib/WorkflowCmggExomecnv.groovy b/lib/WorkflowCmggExomecnv.groovy index f329457..a64bc76 100755 --- a/lib/WorkflowCmggExomecnv.groovy +++ b/lib/WorkflowCmggExomecnv.groovy @@ -1,5 +1,5 @@ // -// This file holds several functions specific to the workflow/nf-cmgg-exomecnv.nf in the CenterForMedicalGeneticsGhent/nf-cmgg-exomecnv pipeline +// This file holds several functions specific to the workflow/nf-cmgg-exomecnv.nf in the nf-cmgg/exomecnv pipeline // import nextflow.Nextflow diff --git a/lib/WorkflowMain.groovy b/lib/WorkflowMain.groovy index 7a10b73..e2e5faa 100755 --- a/lib/WorkflowMain.groovy +++ b/lib/WorkflowMain.groovy @@ -1,5 +1,5 @@ // -// This file holds several functions specific to the main.nf workflow in the CenterForMedicalGeneticsGhent/nf-cmgg-exomecnv pipeline +// This file holds several functions specific to the main.nf workflow in the nf-cmgg/exomecnv pipeline // import nextflow.Nextflow diff --git a/main.nf b/main.nf index 9c00410..ae0ced5 100644 --- a/main.nf +++ b/main.nf @@ -1,9 +1,9 @@ #!/usr/bin/env nextflow /* ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ - CenterForMedicalGeneticsGhent/nf-cmgg-exomecnv + nf-cmgg/exomecnv ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ - Github : https://github.com/CenterForMedicalGeneticsGhent/nf-cmgg-exomecnv + Github : https://github.com/nf-cmgg/exomecnv ---------------------------------------------------------------------------------------- */ @@ -53,7 +53,7 @@ WorkflowMain.initialise(workflow, params, log) include { CMGGEXOMECNV } from './workflows/cmggexomecnv' // -// WORKFLOW: Run main CenterForMedicalGeneticsGhent/nf-cmgg-exomecnv analysis pipeline +// WORKFLOW: Run main nf-cmgg/exomecnv analysis pipeline // workflow CMGG_CMGGEXOMECNV { CMGGEXOMECNV () diff --git a/modules.json b/modules.json index d25326a..4c946e6 100644 --- a/modules.json +++ b/modules.json @@ -1,6 +1,6 @@ { - "name": "CenterForMedicalGeneticsGhent/nf-cmgg-exomecnv", - "homePage": "https://github.com/CenterForMedicalGeneticsGhent/nf-cmgg-exomecnv", + "name": "nf-cmgg/exomecnv", + "homePage": "https://github.com/nf-cmgg/exomecnv", "repos": { "https://github.com/nf-core/modules.git": { "modules": { diff --git a/modules/local/samplesheet_check.nf b/modules/local/samplesheet_check.nf index 77efa85..d1c79c0 100644 --- a/modules/local/samplesheet_check.nf +++ b/modules/local/samplesheet_check.nf @@ -17,7 +17,7 @@ process SAMPLESHEET_CHECK { when: task.ext.when == null || task.ext.when - script: // This script is bundled with the pipeline, in CenterForMedicalGeneticsGhent/nf-cmgg-exomecnv/bin/ + script: // This script is bundled with the pipeline, in nf-cmgg/exomecnv/bin/ """ check_samplesheet.py \\ $samplesheet \\ diff --git a/nextflow.config b/nextflow.config index c19800e..ff1350f 100644 --- a/nextflow.config +++ b/nextflow.config @@ -1,6 +1,6 @@ /* ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ - CenterForMedicalGeneticsGhent/nf-cmgg-exomecnv Nextflow config file + nf-cmgg/exomecnv Nextflow config file ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ Default config options for all compute environments ---------------------------------------------------------------------------------------- @@ -72,7 +72,7 @@ try { System.err.println("WARNING: Could not load nf-core/config profiles: ${params.custom_config_base}/nfcore_custom.config") } -// Load CenterForMedicalGeneticsGhent/nf-cmgg-exomecnv custom profiles from different institutions. +// Load nf-cmgg/exomecnv custom profiles from different institutions. // Warning: Uncomment only if a pipeline-specific instititutional config already exists on nf-core/configs! // try { // includeConfig "${params.custom_config_base}/pipeline/nf-cmgg-exomecnv.config" @@ -230,9 +230,9 @@ dag { } manifest { - name = 'CenterForMedicalGeneticsGhent/nf-cmgg-exomecnv' + name = 'nf-cmgg/exomecnv' author = """nvnieuwk""" - homePage = 'https://github.com/CenterForMedicalGeneticsGhent/nf-cmgg-exomecnv' + homePage = 'https://github.com/nf-cmgg/exomecnv' description = """A nextflow pipeline for calling exome CNVs""" mainScript = 'main.nf' nextflowVersion = '!>=23.04.0' diff --git a/nextflow_schema.json b/nextflow_schema.json index e5a0c17..d76dee2 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -1,7 +1,7 @@ { "$schema": "http://json-schema.org/draft-07/schema", - "$id": "https://raw.githubusercontent.com/CenterForMedicalGeneticsGhent/nf-cmgg-exomecnv/master/nextflow_schema.json", - "title": "CenterForMedicalGeneticsGhent/nf-cmgg-exomecnv pipeline parameters", + "$id": "https://raw.githubusercontent.com/nf-cmgg/exomecnv/master/nextflow_schema.json", + "title": "nf-cmgg/exomecnv pipeline parameters", "description": "A nextflow pipeline for calling exome CNVs", "type": "object", "definitions": { From beee43196e06d80309239f490651ed527c756a5f Mon Sep 17 00:00:00 2001 From: torossee Date: Tue, 5 Mar 2024 09:36:30 +0100 Subject: [PATCH 05/64] samplesheet --- assets/samplesheet.csv | 7 +++--- assets/schema_input.json | 42 +++++++++++++++++++++--------------- assets/sendmail_template.txt | 6 +++--- 3 files changed, 32 insertions(+), 23 deletions(-) diff --git a/assets/samplesheet.csv b/assets/samplesheet.csv index 5f653ab..2bbdf0e 100644 --- a/assets/samplesheet.csv +++ b/assets/samplesheet.csv @@ -1,3 +1,4 @@ -sample,fastq_1,fastq_2 -SAMPLE_PAIRED_END,/path/to/fastq/files/AEG588A1_S1_L002_R1_001.fastq.gz,/path/to/fastq/files/AEG588A1_S1_L002_R2_001.fastq.gz -SAMPLE_SINGLE_END,/path/to/fastq/files/AEG588A4_S4_L003_R1_001.fastq.gz, +sample,pool,family,cram,crai +DNA049572,poolF,Proband_24.03390,/path/to/cram/files/test.cram,/path/to/cram/files/test.cram.crai +NA24385D2_NVQ_034,poolM,Proband_24.03390,/path/to/cram/files/test2.cram,/path/to/cram/files/test2.cram.crai + diff --git a/assets/schema_input.json b/assets/schema_input.json index 86775a3..5e0b2f7 100644 --- a/assets/schema_input.json +++ b/assets/schema_input.json @@ -11,27 +11,35 @@ "type": "string", "pattern": "^\\S+$", "errorMessage": "Sample name must be provided and cannot contain spaces", - "meta": ["id"] + "meta": ["id", "sample"] }, - "fastq_1": { + "pool": { "type": "string", - "pattern": "^\\S+\\.f(ast)?q\\.gz$", - "errorMessage": "FastQ file for reads 1 must be provided, cannot contain spaces and must have extension '.fq.gz' or '.fastq.gz'" + "pattern": "^\\S+$", + "errorMessage": "Pool name must be provided and cannot contain spaces", + "meta": ["pool"] + }, + "family": { + "type": "string", + "pattern": "^\\S+$", + "errorMessage": "Family name must be provided and cannot contain spaces", + "meta": ["family"] }, - "fastq_2": { - "errorMessage": "FastQ file for reads 2 cannot contain spaces and must have extension '.fq.gz' or '.fastq.gz'", - "anyOf": [ - { - "type": "string", - "pattern": "^\\S+\\.f(ast)?q\\.gz$" - }, - { - "type": "string", - "maxLength": 0 - } - ] + "cram": { + "type": "string", + "pattern": "^\\S+\\.(cr|b)am$", + "errorMessage": "CRAM or BAM file must be provided, cannot contain spaces and must have extension '.cram' or '.bam'", + "format": "file-path", + "exists": true + }, + "crai": { + "type": "string", + "pattern": "^\\S+\\.(crai|bai|csi)$", + "errorMessage": "index of CRAM or BAM file must be provided, cannot contain spaces and must have extension '.crai' or '.bai' or '.csi'", + "format": "file-path", + "exists": true } }, - "required": ["sample", "fastq_1"] + "required": ["sample", "pool","family","cram", "crai"] } } diff --git a/assets/sendmail_template.txt b/assets/sendmail_template.txt index 4f0cc10..f701752 100644 --- a/assets/sendmail_template.txt +++ b/assets/sendmail_template.txt @@ -9,12 +9,12 @@ Content-Type: text/html; charset=utf-8 $email_html --nfcoremimeboundary -Content-Type: image/png;name="CenterForMedicalGeneticsGhent-nf-cmgg-exomecnv_logo.png" +Content-Type: image/png;name="nf-cmgg-exomecnv_logo.png" Content-Transfer-Encoding: base64 Content-ID: -Content-Disposition: inline; filename="CenterForMedicalGeneticsGhent-nf-cmgg-exomecnv_logo_light.png" +Content-Disposition: inline; filename="nf-cmgg-exomecnv_logo_light.png" -<% out << new File("$projectDir/assets/CenterForMedicalGeneticsGhent-nf-cmgg-exomecnv_logo_light.png"). +<% out << new File("$projectDir/assets/nf-cmgg-exomecnv_logo_light.png"). bytes. encodeBase64(). toString(). From b22c8a9cb4c3bd13ca0ab1e50e5ae4ac90d9e9f6 Mon Sep 17 00:00:00 2001 From: torossee Date: Tue, 5 Mar 2024 15:06:37 +0100 Subject: [PATCH 06/64] cram to bam --- conf/test.config | 4 ++- main.nf | 8 +++--- .../local/cram_prepare_samtools/main.nf | 28 +++++++++++++++++++ workflows/cmggexomecnv.nf | 20 +++++++------ 4 files changed, 47 insertions(+), 13 deletions(-) create mode 100644 subworkflows/local/cram_prepare_samtools/main.nf diff --git a/conf/test.config b/conf/test.config index 893b94c..8034a6a 100644 --- a/conf/test.config +++ b/conf/test.config @@ -25,5 +25,7 @@ params { input = 'https://raw.githubusercontent.com/nf-core/test-datasets/viralrecon/samplesheet/samplesheet_test_illumina_amplicon.csv' // Genome references - genome = 'R64-1-1' + fasta = 'https://github.com/nf-cmgg/test-datasets/tree/main/data/genomics/homo_sapiens/genome/seq/GCA_000001405.15_GRCh38_full_plus_hs38d1_analysis_set_chr21.fna' + fai = 'https://github.com/nf-cmgg/test-datasets/tree/main/data/genomics/homo_sapiens/genome/seq/GCA_000001405.15_GRCh38_full_plus_hs38d1_analysis_set_chr21.fna.fai' + } diff --git a/main.nf b/main.nf index ae0ced5..749e6c4 100644 --- a/main.nf +++ b/main.nf @@ -14,11 +14,11 @@ nextflow.enable.dsl = 2 GENOME PARAMETER VALUES ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ */ +// use getGenomeAttribute() to fetch parameters +// from igenomes.config using `--genome` -// TODO nf-core: Remove this line if you don't need a FASTA file -// This is an example of how to use getGenomeAttribute() to fetch parameters -// from igenomes.config using `--genome` -// params.fasta = WorkflowMain.getGenomeAttribute(params, 'fasta') +params.fasta = WorkflowMain.getGenomeAttribute(params, 'fasta') +params.fai = WorkflowMain.getGenomeAttribute(params, 'fai') /* ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ diff --git a/subworkflows/local/cram_prepare_samtools/main.nf b/subworkflows/local/cram_prepare_samtools/main.nf new file mode 100644 index 0000000..026d795 --- /dev/null +++ b/subworkflows/local/cram_prepare_samtools/main.nf @@ -0,0 +1,28 @@ + +// Convert CRAM files (optional) +include { SAMTOOLS_CONVERT as CRAM_TO_BAM } from '../../../modules/nf-core/samtools/convert/main' +// include { SAMTOOLS_INDEX } from '../../../modules/nf-core/samtools/index/main' + +workflow CRAM_PREPARE_SAMTOOLS { + + take: + ch_cram // channel: [mandatory] [ val(meta), path(cram), path(crai) ] => sample CRAM files and their indices + fasta // channel: [mandatory] fasta + fasta_fai // channel: [mandatory] fasta_fai + + main: + + ch_versions = Channel.empty() + + // + // convert CRAM files to BAM files + // + CRAM_TO_BAM(ch_cram, fasta, fasta_fai) + ch_versions = ch_versions.mix(CRAM_TO_BAM.out.versions) + + emit: + bam = CRAM_TO_BAM.out.alignment_index // channel: [ val(meta), [ bam ], [bai] ] + + versions = ch_versions // channel: [ versions.yml ] +} + diff --git a/workflows/cmggexomecnv.nf b/workflows/cmggexomecnv.nf index 4b4885f..2796968 100644 --- a/workflows/cmggexomecnv.nf +++ b/workflows/cmggexomecnv.nf @@ -48,6 +48,7 @@ include { BAM_VARIANT_CALLING_EXOMEDEPTH } from '../subworkflows/local/bam_varia // include { FASTQC } from '../modules/nf-core/fastqc/main' include { MULTIQC } from '../modules/nf-core/multiqc/main' include { CUSTOM_DUMPSOFTWAREVERSIONS } from '../modules/nf-core/custom/dumpsoftwareversions/main' +include { CRAM_PREPARE_SAMTOOLS } from '../subworkflows/local/cram_prepare_samtools/main' /* ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ RUN MAIN WORKFLOW @@ -64,16 +65,19 @@ workflow CMGGEXOMECNV { // // SUBWORKFLOW: Read in samplesheet, validate and stage input files // - ch_input = Channel.fromSamplesheet("input") - ch_input.view() - + ch_input_prepare = Channel.fromSamplesheet("input") + ch_input_prepare.view() + // - // MODULE: Run FastQC + // Importing and convert the input files passed through the parameters to channels // - // FASTQC ( - // ch_input - // ) - // ch_versions = ch_versions.mix(FASTQC.out.versions.first()) + ch_fasta = Channel.fromPath(params.fasta).map{ [[id:"reference"], it]}.collect() + ch_fai = params.fai ? Channel.fromPath(params.fai).map{ [[id:"reference"], it]}.collect() : null + + // SUBWORKFLOW: Convert CRAM to BAM + CRAM_PREPARE_SAMTOOLS ( + ch_input_prepare, ch_fasta, ch_fai + ) CUSTOM_DUMPSOFTWAREVERSIONS ( ch_versions.unique().collectFile(name: 'collated_versions.yml') From 67efe38ec57c5cc976db212b3be78296adf8832f Mon Sep 17 00:00:00 2001 From: torossee Date: Wed, 6 Mar 2024 13:16:39 +0100 Subject: [PATCH 07/64] prepare exomedepth count --- assets/schema_input.json | 2 +- bin/CNV_ExomeDepth_counting.R | 106 ++++++++++++++++++ conf/test.config | 4 +- modules/local/exomedepth/count/main.nf | 35 ++++++ .../main.nf | 7 +- .../local/exomedepth_counting/main.nf | 25 +++++ workflows/cmggexomecnv.nf | 24 ++-- 7 files changed, 188 insertions(+), 15 deletions(-) create mode 100644 bin/CNV_ExomeDepth_counting.R create mode 100644 modules/local/exomedepth/count/main.nf rename subworkflows/local/{cram_prepare_samtools => cram_prepare}/main.nf (76%) create mode 100644 subworkflows/local/exomedepth_counting/main.nf diff --git a/assets/schema_input.json b/assets/schema_input.json index 5e0b2f7..66620e6 100644 --- a/assets/schema_input.json +++ b/assets/schema_input.json @@ -40,6 +40,6 @@ "exists": true } }, - "required": ["sample", "pool","family","cram", "crai"] + "required": ["sample", "pool","family","cram"] } } diff --git a/bin/CNV_ExomeDepth_counting.R b/bin/CNV_ExomeDepth_counting.R new file mode 100644 index 0000000..6412008 --- /dev/null +++ b/bin/CNV_ExomeDepth_counting.R @@ -0,0 +1,106 @@ +############################################################################################ +### ExomeDepth - counting step ### +############################################################################################ + +### load libs ### +library("optparse") +library("methods") # to load the function "as" +library("ExomeDepth") +ExomeDepthVersion <- packageDescription("ExomeDepth")$Version +print(paste0("...loaded ExomeDepth version: ", ExomeDepthVersion)) + +## pass options ### +option_list = list( + make_option(c("--bamfile"), type="character", default=NULL, + help="full paths of BAM file", metavar="character"), + make_option(c("--exon_target"), type="character", default=NULL, + help="path to bed file of exon target", metavar="character"), + make_option(c("--prefix"), type="character", default=NULL, + help="prefix for output name, chrX or autosomal", metavar="character") +); +opt_parser = OptionParser(option_list=option_list); +opt = parse_args(opt_parser); + +if (is.null(opt$bamfile)){ + print_help(opt_parser) + stop("Not all the input arguments are provided", call.=FALSE) +} +if (is.null(opt$exon_target)){ + print_help(opt_parser) + stop("Not all the input arguments are provided", call.=FALSE) +} +if (is.null(opt$prefix)){ + print_help(opt_parser) + stop("Not all the input arguments are provided", call.=FALSE) +} + +cat("bam file: ", opt$bamfile, "\n") +cat("exon target file: ", opt$exon_target, "\n") +cat("prefix: ", opt$prefix, "\n") + +### load exon data ### +exons <- read.table(file=opt$exon_target,sep="\t",header = F) +colnames(exons) <- c("chromosome","start","end","name") + +### load all exon info #### +exons.GRanges <- GenomicRanges::GRanges(seqnames = exons$chromosome, + IRanges::IRanges(start=exons$start,end=exons$end), + names = exons$name + ) + +### Getting the input data from BAM file ### +bam <- unlist(opt$bamfile ) + +sampleName <- unlist(strsplit(unlist(strsplit(bam, ".bam")),split=".//")) + +cat("Sample names:", "\n") +sampleName2<-gsub("^.*/","",sampleName) +sampleName2 + +### ExomeDepth sometimes spectacularly fails to find the BAM index, for no clear reason ### +## so help it out by testing a few likely filenames +bamindex = bam + +stardotbai = gsub(".bam$",".bai",bam) +stardotbamdotbai = gsub(".bam$",".bam.bai",bam) +if (file.exists(stardotbai)) { + bamindexe = stardotbai +} else if (file.exists(stardotbamdotbai)) { + bamindexe = stardotbamdotbai +} +else { + cat(paste("Cannot find a .bai index for BAM: ",bam,"\n",sep=""),file=stderr()) + cat("stopping execution....",file=stderr()) + stop() +} + +### read.count ~exomeCopy package ### +## ouput = GenomicRages object that stores the read count data form the BAM file ### +cat("\nCalling ExomeDepth to compute counts...\n") +ExomeCount <- getBamCounts(bed.frame = exons, + bam.files = bam, #vector of BAM path strings + include.chr = TRUE + #referenceFasta = fasta #only useful if one wants to obtain the GC content + #min.mapq =20 #default minimum mapping quality to include a read + #read.width =300 #default maximum distance between the side of the target region and the middle of the paired reads to include the paired read into that region + ) +## convert GRanges class (S4 object) into a data.frame, which is the input format for ExomeDepth +ExomeCount.dafr <- as(ExomeCount[, colnames(ExomeCount)], 'data.frame') +## remove the annoying chr letters +ExomeCount.dafr$space <- gsub(as.character(ExomeCount.dafr$space), + pattern = 'chr', + replacement = '') +## rename sample names in colnames +header_first_part<-c("space", "start", "end", "width", "names") +colnames(ExomeCount.dafr)<-c(header_first_part, sampleName2) +colnames(ExomeCount.dafr) +print(head(ExomeCount.dafr)) +cat("Successfully calculated counts.\n") + +### save counts as a text file and rda object ### +cat("Saving the counts \n") +countspath = paste("counts_",sampleName2,"_",opt$prefix,".txt",sep="") +write.table(ExomeCount.dafr,countspath,sep="\t",col.names=TRUE,row.names=FALSE,quote=FALSE) + +cat("\n\n---Finished---\n") + diff --git a/conf/test.config b/conf/test.config index 8034a6a..199ac17 100644 --- a/conf/test.config +++ b/conf/test.config @@ -27,5 +27,7 @@ params { // Genome references fasta = 'https://github.com/nf-cmgg/test-datasets/tree/main/data/genomics/homo_sapiens/genome/seq/GCA_000001405.15_GRCh38_full_plus_hs38d1_analysis_set_chr21.fna' fai = 'https://github.com/nf-cmgg/test-datasets/tree/main/data/genomics/homo_sapiens/genome/seq/GCA_000001405.15_GRCh38_full_plus_hs38d1_analysis_set_chr21.fna.fai' - + + // Parameters + cram = true } diff --git a/modules/local/exomedepth/count/main.nf b/modules/local/exomedepth/count/main.nf new file mode 100644 index 0000000..2cd3d6d --- /dev/null +++ b/modules/local/exomedepth/count/main.nf @@ -0,0 +1,35 @@ +process EXOMEDEPTH_COUNT { + tag "$meta.id $prefix" + label 'process_low' + + conda "conda-forge::r=3.6" + container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? + 'https://depot.galaxyproject.org/singularity/r-exomedepth:1.1.12--r36h6786f55_0' : + 'biocontainers/r-exomedepth:1.1.12--r36h6786f55_0' }" + + input: + tuple val(meta), path(bam), path(bai) + tuple val(prefix), path(exon_target) + + output: + path '*.txt' , emit: counts + path "versions.yml", emit: versions + + when: + task.ext.when == null || task.ext.when + + script: // This script is bundled with the pipeline, in nf-cmgg/exomecnv/bin/ + + def VERSION = '1.1.12' + + """ + Rscript CNV_ExomeDepth_counting.R + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + ExomeDepth: ${VERSION} + R: \$(Rscript --version | sed 's/R scripting front-end //g') + END_VERSIONS + + """ +} diff --git a/subworkflows/local/cram_prepare_samtools/main.nf b/subworkflows/local/cram_prepare/main.nf similarity index 76% rename from subworkflows/local/cram_prepare_samtools/main.nf rename to subworkflows/local/cram_prepare/main.nf index 026d795..1a6da4a 100644 --- a/subworkflows/local/cram_prepare_samtools/main.nf +++ b/subworkflows/local/cram_prepare/main.nf @@ -1,12 +1,11 @@ -// Convert CRAM files (optional) +// Convert CRAM files include { SAMTOOLS_CONVERT as CRAM_TO_BAM } from '../../../modules/nf-core/samtools/convert/main' -// include { SAMTOOLS_INDEX } from '../../../modules/nf-core/samtools/index/main' -workflow CRAM_PREPARE_SAMTOOLS { +workflow CRAM_PREPARE { take: - ch_cram // channel: [mandatory] [ val(meta), path(cram), path(crai) ] => sample CRAM files and their indices + ch_cram // channel: [mandatory] [ val(meta), path(cram), path(crai) ] => sample CRAM files and their optional indices fasta // channel: [mandatory] fasta fasta_fai // channel: [mandatory] fasta_fai diff --git a/subworkflows/local/exomedepth_counting/main.nf b/subworkflows/local/exomedepth_counting/main.nf new file mode 100644 index 0000000..3506067 --- /dev/null +++ b/subworkflows/local/exomedepth_counting/main.nf @@ -0,0 +1,25 @@ + +// Convert CRAM files + +workflow EXOMEDEPTH_COUNTING { + + take: + ch_bam // channel: [mandatory] [ val(meta), path(bam), path(bai) ] => sample BAM files and their indices + exon_target // channel: [mandatory] [ val(meta), path(bed) ] + + main: + + ch_versions = Channel.empty() + + // + // convert CRAM files to BAM files + // + CRAM_TO_BAM(ch_cram, fasta, fasta_fai) + ch_versions = ch_versions.mix(CRAM_TO_BAM.out.versions) + + emit: + bam = CRAM_TO_BAM.out.alignment_index // channel: [ val(meta), [ bam ], [bai] ] + + versions = ch_versions // channel: [ versions.yml ] +} + diff --git a/workflows/cmggexomecnv.nf b/workflows/cmggexomecnv.nf index 2796968..fe82871 100644 --- a/workflows/cmggexomecnv.nf +++ b/workflows/cmggexomecnv.nf @@ -48,7 +48,7 @@ include { BAM_VARIANT_CALLING_EXOMEDEPTH } from '../subworkflows/local/bam_varia // include { FASTQC } from '../modules/nf-core/fastqc/main' include { MULTIQC } from '../modules/nf-core/multiqc/main' include { CUSTOM_DUMPSOFTWAREVERSIONS } from '../modules/nf-core/custom/dumpsoftwareversions/main' -include { CRAM_PREPARE_SAMTOOLS } from '../subworkflows/local/cram_prepare_samtools/main' +include { CRAM_PREPARE } from '../subworkflows/local/cram_prepare/main' /* ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ RUN MAIN WORKFLOW @@ -67,18 +67,24 @@ workflow CMGGEXOMECNV { // ch_input_prepare = Channel.fromSamplesheet("input") ch_input_prepare.view() - + // // Importing and convert the input files passed through the parameters to channels // - ch_fasta = Channel.fromPath(params.fasta).map{ [[id:"reference"], it]}.collect() - ch_fai = params.fai ? Channel.fromPath(params.fai).map{ [[id:"reference"], it]}.collect() : null - - // SUBWORKFLOW: Convert CRAM to BAM - CRAM_PREPARE_SAMTOOLS ( - ch_input_prepare, ch_fasta, ch_fai - ) + // SUBWORKFLOW: Convert CRAM to BAM if no BAM file was provided + if (params.cram){ + + ch_fasta = Channel.fromPath(params.fasta).map{ [[id:"reference"], it]}.collect() + ch_fai = params.fai ? Channel.fromPath(params.fai).map{ [[id:"reference"], it]}.collect() : null + + CRAM_PREPARE ( + ch_input_prepare, ch_fasta, ch_fai + ) + CRAM_PREPARE.out.bam.view() + } + // + CUSTOM_DUMPSOFTWAREVERSIONS ( ch_versions.unique().collectFile(name: 'collated_versions.yml') ) From 81413df34e80ccb6494e7da50b01dda396947d19 Mon Sep 17 00:00:00 2001 From: torossee Date: Fri, 8 Mar 2024 13:45:55 +0100 Subject: [PATCH 08/64] deal with bam and cram inputs --- conf/test.config | 2 +- workflows/cmggexomecnv.nf | 38 ++++++++++++++++++++++++-------------- 2 files changed, 25 insertions(+), 15 deletions(-) diff --git a/conf/test.config b/conf/test.config index 199ac17..a2c12e1 100644 --- a/conf/test.config +++ b/conf/test.config @@ -29,5 +29,5 @@ params { fai = 'https://github.com/nf-cmgg/test-datasets/tree/main/data/genomics/homo_sapiens/genome/seq/GCA_000001405.15_GRCh38_full_plus_hs38d1_analysis_set_chr21.fna.fai' // Parameters - cram = true + } diff --git a/workflows/cmggexomecnv.nf b/workflows/cmggexomecnv.nf index fe82871..f27d322 100644 --- a/workflows/cmggexomecnv.nf +++ b/workflows/cmggexomecnv.nf @@ -35,6 +35,7 @@ ch_multiqc_custom_methods_description = params.multiqc_methods_description ? fil // // SUBWORKFLOW: Consisting of a mix of local and nf-core/modules // +include { CRAM_PREPARE } from '../subworkflows/local/cram_prepare/main' include { BAM_VARIANT_CALLING_EXOMEDEPTH } from '../subworkflows/local/bam_variant_calling_exomedepth/main' /* ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ @@ -48,7 +49,6 @@ include { BAM_VARIANT_CALLING_EXOMEDEPTH } from '../subworkflows/local/bam_varia // include { FASTQC } from '../modules/nf-core/fastqc/main' include { MULTIQC } from '../modules/nf-core/multiqc/main' include { CUSTOM_DUMPSOFTWAREVERSIONS } from '../modules/nf-core/custom/dumpsoftwareversions/main' -include { CRAM_PREPARE } from '../subworkflows/local/cram_prepare/main' /* ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ RUN MAIN WORKFLOW @@ -65,25 +65,35 @@ workflow CMGGEXOMECNV { // // SUBWORKFLOW: Read in samplesheet, validate and stage input files // - ch_input_prepare = Channel.fromSamplesheet("input") - ch_input_prepare.view() + ch_input = Channel.fromSamplesheet("input") + // ch_input.view() + + // branch into CRAM and BAM files + ch_input.branch { meta, cram, crai -> + CRAM: cram.extension == "cram" + BAM: cram.extension == "bam" + } + .set{ ch_input_prepare } + // ch_input_prepare.BAM.view { "BAM: $it" } + // ch_input_prepare.CRAM.view { "CRAM: $it" } // // Importing and convert the input files passed through the parameters to channels // // SUBWORKFLOW: Convert CRAM to BAM if no BAM file was provided - if (params.cram){ - - ch_fasta = Channel.fromPath(params.fasta).map{ [[id:"reference"], it]}.collect() - ch_fai = params.fai ? Channel.fromPath(params.fai).map{ [[id:"reference"], it]}.collect() : null - - CRAM_PREPARE ( - ch_input_prepare, ch_fasta, ch_fai - ) - CRAM_PREPARE.out.bam.view() - } - // + ch_fasta = Channel.fromPath(params.fasta).map{ [[id:"reference"], it]}.collect() + ch_fai = params.fai ? Channel.fromPath(params.fai).map{ [[id:"reference"], it]}.collect() : null + + CRAM_PREPARE ( + ch_input_prepare.CRAM, ch_fasta, ch_fai + ) + + ch_input_prepare.BAM + .mix(CRAM_PREPARE.out.bam) + .set{ ch_input_bam } + + ch_input_bam.view() CUSTOM_DUMPSOFTWAREVERSIONS ( ch_versions.unique().collectFile(name: 'collated_versions.yml') From 36fc6045eb896e9c1caf168e9e6eaa8499e30606 Mon Sep 17 00:00:00 2001 From: torossee Date: Fri, 8 Mar 2024 14:00:53 +0100 Subject: [PATCH 09/64] fix yaml --- .nf-core.yml | 10 ++-------- 1 file changed, 2 insertions(+), 8 deletions(-) diff --git a/.nf-core.yml b/.nf-core.yml index bfcaa49..47168a3 100644 --- a/.nf-core.yml +++ b/.nf-core.yml @@ -1,17 +1,11 @@ lint: files_exist: - CODE_OF_CONDUCT.md - - assets/nf-core-nf-cmgg-exomecnv_logo_light.png - - docs/images/nf-core-nf-cmgg-exomecnv_logo_light.png - - docs/images/nf-core-nf-cmgg-exomecnv_logo_dark.png - .github/ISSUE_TEMPLATE/config.yml - .github/workflows/awstest.yml - .github/workflows/awsfulltest.yml files_unchanged: - CODE_OF_CONDUCT.md - - assets/nf-core-nf-cmgg-exomecnv_logo_light.png - - docs/images/nf-core-nf-cmgg-exomecnv_logo_light.png - - docs/images/nf-core-nf-cmgg-exomecnv_logo_dark.png - .github/ISSUE_TEMPLATE/bug_report.yml multiqc_config: - report_comment @@ -22,5 +16,5 @@ repository_type: pipeline template: author: nvnieuwk description: A nextflow pipeline for calling exome CNVs - name: nf-cmgg-exomecnv - prefix: CenterForMedicalGeneticsGhent + name: exomecnv + prefix: nf-cmgg From d44fe62e0aea9a3b154d6995bf46cb54aae242e3 Mon Sep 17 00:00:00 2001 From: torossee Date: Fri, 8 Mar 2024 14:01:15 +0100 Subject: [PATCH 10/64] Template update for nf-core/tools version 2.13.1 --- .devcontainer/devcontainer.json | 10 +- .editorconfig | 15 +- .github/CONTRIBUTING.md | 25 +- .github/ISSUE_TEMPLATE/bug_report.yml | 101 ++-- .github/ISSUE_TEMPLATE/feature_request.yml | 2 +- .github/PULL_REQUEST_TEMPLATE.md | 10 +- .github/workflows/branch.yml | 6 +- .github/workflows/ci.yml | 7 +- .github/workflows/clean-up.yml | 2 +- .github/workflows/download_pipeline.yml | 72 +++ .github/workflows/fix-linting.yml | 74 ++- .github/workflows/linting.yml | 67 +-- .github/workflows/linting_comment.yml | 4 +- ...uncments.yml => release-announcements.yml} | 11 +- .gitpod.yml | 9 +- .nf-core.yml | 34 +- .pre-commit-config.yaml | 7 +- CHANGELOG.md | 4 +- CITATIONS.md | 2 +- README.md | 30 +- assets/adaptivecard.json | 2 +- assets/email_template.html | 14 +- assets/email_template.txt | 8 +- assets/methods_description_template.yml | 8 +- assets/multiqc_config.yml | 8 +- assets/schema_input.json | 25 +- assets/sendmail_template.txt | 6 +- assets/slackreport.json | 2 +- bin/check_samplesheet.py | 259 ----------- conf/base.config | 2 +- conf/modules.config | 8 - conf/test.config | 2 +- conf/test_full.config | 2 +- docs/README.md | 4 +- docs/output.md | 2 +- docs/usage.md | 10 +- lib/NfcoreTemplate.groovy | 351 -------------- lib/Utils.groovy | 47 -- lib/WorkflowMain.groovy | 63 --- lib/WorkflowNf-cmgg-exomecnv.groovy | 122 ----- lib/nfcore_external_java_deps.jar | Bin 2291171 -> 0 bytes main.nf | 104 +++-- modules.json | 32 +- modules/local/samplesheet_check.nf | 31 -- .../dumpsoftwareversions/environment.yml | 7 - .../custom/dumpsoftwareversions/main.nf | 24 - .../custom/dumpsoftwareversions/meta.yml | 37 -- .../templates/dumpsoftwareversions.py | 102 ---- .../dumpsoftwareversions/tests/main.nf.test | 38 -- .../tests/main.nf.test.snap | 27 -- .../dumpsoftwareversions/tests/tags.yml | 2 - modules/nf-core/fastqc/main.nf | 4 +- modules/nf-core/fastqc/tests/main.nf.test | 197 +++++++- .../nf-core/fastqc/tests/main.nf.test.snap | 82 +++- modules/nf-core/multiqc/environment.yml | 4 +- modules/nf-core/multiqc/main.nf | 8 +- modules/nf-core/multiqc/meta.yml | 3 +- modules/nf-core/multiqc/tests/main.nf.test | 84 ++++ .../nf-core/multiqc/tests/main.nf.test.snap | 41 ++ modules/nf-core/multiqc/tests/tags.yml | 2 + nextflow.config | 27 +- nextflow_schema.json | 5 +- pyproject.toml | 19 +- subworkflows/local/input_check.nf | 44 -- .../utils_nfcore_exomecnv_pipeline/main.nf | 251 ++++++++++ .../nf-core/utils_nextflow_pipeline/main.nf | 126 +++++ .../nf-core/utils_nextflow_pipeline/meta.yml | 38 ++ .../tests/main.function.nf.test | 54 +++ .../tests/main.function.nf.test.snap | 20 + .../tests/main.workflow.nf.test | 111 +++++ .../tests/nextflow.config | 9 + .../utils_nextflow_pipeline/tests/tags.yml | 2 + .../nf-core/utils_nfcore_pipeline/main.nf | 440 ++++++++++++++++++ .../nf-core/utils_nfcore_pipeline/meta.yml | 24 + .../tests/main.function.nf.test | 134 ++++++ .../tests/main.function.nf.test.snap | 166 +++++++ .../tests/main.workflow.nf.test | 29 ++ .../tests/main.workflow.nf.test.snap | 19 + .../tests/nextflow.config | 9 + .../utils_nfcore_pipeline/tests/tags.yml | 2 + .../nf-core/utils_nfvalidation_plugin/main.nf | 62 +++ .../utils_nfvalidation_plugin/meta.yml | 44 ++ .../tests/main.nf.test | 200 ++++++++ .../tests/nextflow_schema.json | 96 ++++ .../utils_nfvalidation_plugin/tests/tags.yml | 2 + workflows/exomecnv.nf | 76 +++ workflows/nf-cmgg-exomecnv.nf | 134 ------ 87 files changed, 2768 insertions(+), 1641 deletions(-) create mode 100644 .github/workflows/download_pipeline.yml rename .github/workflows/{release-announcments.yml => release-announcements.yml} (80%) delete mode 100755 bin/check_samplesheet.py delete mode 100755 lib/NfcoreTemplate.groovy delete mode 100644 lib/Utils.groovy delete mode 100755 lib/WorkflowMain.groovy delete mode 100755 lib/WorkflowNf-cmgg-exomecnv.groovy delete mode 100644 lib/nfcore_external_java_deps.jar delete mode 100644 modules/local/samplesheet_check.nf delete mode 100644 modules/nf-core/custom/dumpsoftwareversions/environment.yml delete mode 100644 modules/nf-core/custom/dumpsoftwareversions/main.nf delete mode 100644 modules/nf-core/custom/dumpsoftwareversions/meta.yml delete mode 100755 modules/nf-core/custom/dumpsoftwareversions/templates/dumpsoftwareversions.py delete mode 100644 modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test delete mode 100644 modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test.snap delete mode 100644 modules/nf-core/custom/dumpsoftwareversions/tests/tags.yml create mode 100644 modules/nf-core/multiqc/tests/main.nf.test create mode 100644 modules/nf-core/multiqc/tests/main.nf.test.snap create mode 100644 modules/nf-core/multiqc/tests/tags.yml delete mode 100644 subworkflows/local/input_check.nf create mode 100644 subworkflows/local/utils_nfcore_exomecnv_pipeline/main.nf create mode 100644 subworkflows/nf-core/utils_nextflow_pipeline/main.nf create mode 100644 subworkflows/nf-core/utils_nextflow_pipeline/meta.yml create mode 100644 subworkflows/nf-core/utils_nextflow_pipeline/tests/main.function.nf.test create mode 100644 subworkflows/nf-core/utils_nextflow_pipeline/tests/main.function.nf.test.snap create mode 100644 subworkflows/nf-core/utils_nextflow_pipeline/tests/main.workflow.nf.test create mode 100644 subworkflows/nf-core/utils_nextflow_pipeline/tests/nextflow.config create mode 100644 subworkflows/nf-core/utils_nextflow_pipeline/tests/tags.yml create mode 100644 subworkflows/nf-core/utils_nfcore_pipeline/main.nf create mode 100644 subworkflows/nf-core/utils_nfcore_pipeline/meta.yml create mode 100644 subworkflows/nf-core/utils_nfcore_pipeline/tests/main.function.nf.test create mode 100644 subworkflows/nf-core/utils_nfcore_pipeline/tests/main.function.nf.test.snap create mode 100644 subworkflows/nf-core/utils_nfcore_pipeline/tests/main.workflow.nf.test create mode 100644 subworkflows/nf-core/utils_nfcore_pipeline/tests/main.workflow.nf.test.snap create mode 100644 subworkflows/nf-core/utils_nfcore_pipeline/tests/nextflow.config create mode 100644 subworkflows/nf-core/utils_nfcore_pipeline/tests/tags.yml create mode 100644 subworkflows/nf-core/utils_nfvalidation_plugin/main.nf create mode 100644 subworkflows/nf-core/utils_nfvalidation_plugin/meta.yml create mode 100644 subworkflows/nf-core/utils_nfvalidation_plugin/tests/main.nf.test create mode 100644 subworkflows/nf-core/utils_nfvalidation_plugin/tests/nextflow_schema.json create mode 100644 subworkflows/nf-core/utils_nfvalidation_plugin/tests/tags.yml create mode 100644 workflows/exomecnv.nf delete mode 100644 workflows/nf-cmgg-exomecnv.nf diff --git a/.devcontainer/devcontainer.json b/.devcontainer/devcontainer.json index 4ecfbfe..b290e09 100644 --- a/.devcontainer/devcontainer.json +++ b/.devcontainer/devcontainer.json @@ -10,15 +10,7 @@ "vscode": { // Set *default* container specific settings.json values on container create. "settings": { - "python.defaultInterpreterPath": "/opt/conda/bin/python", - "python.linting.enabled": true, - "python.linting.pylintEnabled": true, - "python.formatting.autopep8Path": "/opt/conda/bin/autopep8", - "python.formatting.yapfPath": "/opt/conda/bin/yapf", - "python.linting.flake8Path": "/opt/conda/bin/flake8", - "python.linting.pycodestylePath": "/opt/conda/bin/pycodestyle", - "python.linting.pydocstylePath": "/opt/conda/bin/pydocstyle", - "python.linting.pylintPath": "/opt/conda/bin/pylint" + "python.defaultInterpreterPath": "/opt/conda/bin/python" }, // Add the IDs of extensions you want installed when the container is created. diff --git a/.editorconfig b/.editorconfig index b6b3190..dd9ffa5 100644 --- a/.editorconfig +++ b/.editorconfig @@ -18,7 +18,20 @@ end_of_line = unset insert_final_newline = unset trim_trailing_whitespace = unset indent_style = unset -indent_size = unset +[/subworkflows/nf-core/**] +charset = unset +end_of_line = unset +insert_final_newline = unset +trim_trailing_whitespace = unset +indent_style = unset [/assets/email*] indent_size = unset + +# ignore Readme +[README.md] +indent_style = unset + +# ignore python +[*.{py,md}] +indent_style = unset diff --git a/.github/CONTRIBUTING.md b/.github/CONTRIBUTING.md index d4ecde9..d0a47c0 100644 --- a/.github/CONTRIBUTING.md +++ b/.github/CONTRIBUTING.md @@ -1,9 +1,9 @@ -# CenterForMedicalGeneticsGhent/nf-cmgg-exomecnv: Contributing Guidelines +# nf-cmgg/exomecnv: Contributing Guidelines Hi there! -Many thanks for taking an interest in improving CenterForMedicalGeneticsGhent/nf-cmgg-exomecnv. +Many thanks for taking an interest in improving nf-cmgg/exomecnv. -We try to manage the required tasks for CenterForMedicalGeneticsGhent/nf-cmgg-exomecnv using GitHub issues, you probably came to this page when creating one. +We try to manage the required tasks for nf-cmgg/exomecnv using GitHub issues, you probably came to this page when creating one. Please use the pre-filled template to save time. However, don't be put off by this template - other more general issues and suggestions are welcome! @@ -11,10 +11,10 @@ Contributions to the code are even more welcome ;) ## Contribution workflow -If you'd like to write some code for CenterForMedicalGeneticsGhent/nf-cmgg-exomecnv, the standard workflow is as follows: +If you'd like to write some code for nf-cmgg/exomecnv, the standard workflow is as follows: -1. Check that there isn't already an issue about your idea in the [CenterForMedicalGeneticsGhent/nf-cmgg-exomecnv issues](https://github.com/CenterForMedicalGeneticsGhent/nf-cmgg-exomecnv/issues) to avoid duplicating work. If there isn't one already, please create one so that others know you're working on this -2. [Fork](https://help.github.com/en/github/getting-started-with-github/fork-a-repo) the [CenterForMedicalGeneticsGhent/nf-cmgg-exomecnv repository](https://github.com/CenterForMedicalGeneticsGhent/nf-cmgg-exomecnv) to your GitHub account +1. Check that there isn't already an issue about your idea in the [nf-cmgg/exomecnv issues](https://github.com/nf-cmgg/exomecnv/issues) to avoid duplicating work. If there isn't one already, please create one so that others know you're working on this +2. [Fork](https://help.github.com/en/github/getting-started-with-github/fork-a-repo) the [nf-cmgg/exomecnv repository](https://github.com/nf-cmgg/exomecnv) to your GitHub account 3. Make the necessary changes / additions within your forked repository following [Pipeline conventions](#pipeline-contribution-conventions) 4. Use `nf-core schema build` and add any new parameters to the pipeline JSON schema (requires [nf-core tools](https://github.com/nf-core/tools) >= 1.10). 5. Submit a Pull Request against the `dev` branch and wait for the code to be reviewed and merged @@ -23,8 +23,11 @@ If you're not used to this workflow with git, you can start with some [docs from ## Tests -You can optionally test your changes by running the pipeline locally. Then it is recommended to use the `debug` profile to -receive warnings about process selectors and other debug info. Example: `nextflow run . -profile debug,test,docker --outdir `. +You have the option to test your changes locally by running the pipeline. For receiving warnings about process selectors and other `debug` information, it is recommended to use the debug profile. Execute all the tests with the following command: + +```bash +nf-test test --profile debug,test,docker --verbose +``` When you create a pull request with changes, [GitHub Actions](https://github.com/features/actions) will run automatic tests. Typically, pull-requests are only fully reviewed when these tests are passing, though of course we can help out before then. @@ -55,7 +58,7 @@ These tests are run both with the latest available version of `Nextflow` and als ## Pipeline contribution conventions -To make the CenterForMedicalGeneticsGhent/nf-cmgg-exomecnv code and processing logic more understandable for new contributors and to ensure quality, we semi-standardise the way the code and other contributions are written. +To make the nf-cmgg/exomecnv code and processing logic more understandable for new contributors and to ensure quality, we semi-standardise the way the code and other contributions are written. ### Adding a new step @@ -82,7 +85,7 @@ Once there, use `nf-core schema build` to add to `nextflow_schema.json`. Sensible defaults for process resource requirements (CPUs / memory / time) for a process should be defined in `conf/base.config`. These should generally be specified generic with `withLabel:` selectors so they can be shared across multiple processes/steps of the pipeline. A nf-core standard set of labels that should be followed where possible can be seen in the [nf-core pipeline template](https://github.com/nf-core/tools/blob/master/nf_core/pipeline-template/conf/base.config), which has the default process as a single core-process, and then different levels of multi-core configurations for increasingly large memory requirements defined with standardised labels. -The process resources can be passed on to the tool dynamically within the process with the `${task.cpu}` and `${task.memory}` variables in the `script:` block. +The process resources can be passed on to the tool dynamically within the process with the `${task.cpus}` and `${task.memory}` variables in the `script:` block. ### Naming schemes @@ -105,7 +108,7 @@ This repo includes a devcontainer configuration which will create a GitHub Codes To get started: -- Open the repo in [Codespaces](https://github.com/CenterForMedicalGeneticsGhent/nf-cmgg-exomecnv/codespaces) +- Open the repo in [Codespaces](https://github.com/nf-cmgg/exomecnv/codespaces) - Tools installed - nf-core - Nextflow diff --git a/.github/ISSUE_TEMPLATE/bug_report.yml b/.github/ISSUE_TEMPLATE/bug_report.yml index 487b21c..349f81c 100644 --- a/.github/ISSUE_TEMPLATE/bug_report.yml +++ b/.github/ISSUE_TEMPLATE/bug_report.yml @@ -2,54 +2,53 @@ name: Bug report description: Report something that is broken or incorrect labels: bug body: -- type: textarea - id: description - attributes: - label: Description of the bug - description: A clear and concise description of what the bug is. - validations: - required: true -- type: textarea - id: command_used - attributes: - label: Command used and terminal output - description: Steps to reproduce the behaviour. Please paste the command you used - to launch the pipeline and the output from your terminal. - render: console - placeholder: '$ nextflow run ... - - - Some output where something broke - - ' -- type: textarea - id: files - attributes: - label: Relevant files - description: 'Please drag and drop the relevant files here. Create a `.zip` archive - if the extension is not allowed. - - Your verbose log file `.nextflow.log` is often useful _(this is a hidden file - in the directory where you launched the pipeline)_ as well as custom Nextflow - configuration files. - - ' -- type: textarea - id: system - attributes: - label: System information - description: '* Nextflow version _(eg. 23.04.0)_ - - * Hardware _(eg. HPC, Desktop, Cloud)_ - - * Executor _(eg. slurm, local, awsbatch)_ - - * Container engine: _(e.g. Docker, Singularity, Conda, Podman, Shifter, Charliecloud, - or Apptainer)_ - - * OS _(eg. CentOS Linux, macOS, Linux Mint)_ - - * Version of CenterForMedicalGeneticsGhent/nf-cmgg-exomecnv _(eg. 1.1, 1.5, - 1.8.2)_ - - ' + - type: textarea + id: description + attributes: + label: Description of the bug + description: A clear and concise description of what the bug is. + validations: + required: true + - type: textarea + id: command_used + attributes: + label: Command used and terminal output + description: Steps to reproduce the behaviour. Please paste the command you used + to launch the pipeline and the output from your terminal. + render: console + placeholder: "$ nextflow run ... + + + Some output where something broke + + " + - type: textarea + id: files + attributes: + label: Relevant files + description: "Please drag and drop the relevant files here. Create a `.zip` archive + if the extension is not allowed. + + Your verbose log file `.nextflow.log` is often useful _(this is a hidden file + in the directory where you launched the pipeline)_ as well as custom Nextflow + configuration files. + + " + - type: textarea + id: system + attributes: + label: System information + description: "* Nextflow version _(eg. 23.04.0)_ + + * Hardware _(eg. HPC, Desktop, Cloud)_ + + * Executor _(eg. slurm, local, awsbatch)_ + + * Container engine: _(e.g. Docker, Singularity, Conda, Podman, Shifter, Charliecloud, + or Apptainer)_ + + * OS _(eg. CentOS Linux, macOS, Linux Mint)_ + + * Version of nf-cmgg/exomecnv _(eg. 1.1, 1.5, 1.8.2)_ + + " diff --git a/.github/ISSUE_TEMPLATE/feature_request.yml b/.github/ISSUE_TEMPLATE/feature_request.yml index f2536f6..e88bf0b 100644 --- a/.github/ISSUE_TEMPLATE/feature_request.yml +++ b/.github/ISSUE_TEMPLATE/feature_request.yml @@ -1,5 +1,5 @@ name: Feature request -description: Suggest an idea for the CenterForMedicalGeneticsGhent/nf-cmgg-exomecnv pipeline +description: Suggest an idea for the nf-cmgg/exomecnv pipeline labels: enhancement body: - type: textarea diff --git a/.github/PULL_REQUEST_TEMPLATE.md b/.github/PULL_REQUEST_TEMPLATE.md index cb3c5b6..2ccb3c0 100644 --- a/.github/PULL_REQUEST_TEMPLATE.md +++ b/.github/PULL_REQUEST_TEMPLATE.md @@ -1,23 +1,23 @@ ## PR checklist - [ ] This comment contains a description of changes (with reason). - [ ] If you've fixed a bug or added code that should be tested, add tests! -- [ ] If you've added a new tool - have you followed the pipeline conventions in the [contribution docs](https://github.com/CenterForMedicalGeneticsGhent/nf-cmgg-exomecnv/tree/master/.github/CONTRIBUTING.md) +- [ ] If you've added a new tool - have you followed the pipeline conventions in the [contribution docs](https://github.com/nf-cmgg/exomecnv/tree/master/.github/CONTRIBUTING.md) - [ ] Make sure your code lints (`nf-core lint`). -- [ ] Ensure the test suite passes (`nextflow run . -profile test,docker --outdir `). +- [ ] Ensure the test suite passes (`nf-test test main.nf.test -profile test,docker`). - [ ] Check for unexpected warnings in debug mode (`nextflow run . -profile debug,test,docker --outdir `). - [ ] Usage Documentation in `docs/usage.md` is updated. - [ ] Output Documentation in `docs/output.md` is updated. diff --git a/.github/workflows/branch.yml b/.github/workflows/branch.yml index 5637b58..617f961 100644 --- a/.github/workflows/branch.yml +++ b/.github/workflows/branch.yml @@ -11,15 +11,15 @@ jobs: steps: # PRs to the nf-core repo master branch are only ok if coming from the nf-core repo `dev` or any `patch` branches - name: Check PRs - if: github.repository == 'CenterForMedicalGeneticsGhent/nf-cmgg-exomecnv' + if: github.repository == 'nf-cmgg/exomecnv' run: | - { [[ ${{github.event.pull_request.head.repo.full_name }} == CenterForMedicalGeneticsGhent/nf-cmgg-exomecnv ]] && [[ $GITHUB_HEAD_REF == "dev" ]]; } || [[ $GITHUB_HEAD_REF == "patch" ]] + { [[ ${{github.event.pull_request.head.repo.full_name }} == nf-cmgg/exomecnv ]] && [[ $GITHUB_HEAD_REF == "dev" ]]; } || [[ $GITHUB_HEAD_REF == "patch" ]] # If the above check failed, post a comment on the PR explaining the failure # NOTE - this doesn't currently work if the PR is coming from a fork, due to limitations in GitHub actions secrets - name: Post PR comment if: failure() - uses: mshick/add-pr-comment@v1 + uses: mshick/add-pr-comment@b8f338c590a895d50bcbfa6c5859251edc8952fc # v2 with: message: | ## This PR is against the `master` branch :x: diff --git a/.github/workflows/ci.yml b/.github/workflows/ci.yml index 0e9155a..0cdd2da 100644 --- a/.github/workflows/ci.yml +++ b/.github/workflows/ci.yml @@ -19,7 +19,7 @@ jobs: test: name: Run pipeline with test data # Only run on push if this is the nf-core dev branch (merged PRs) - if: "${{ github.event_name != 'push' || (github.event_name == 'push' && github.repository == 'CenterForMedicalGeneticsGhent/nf-cmgg-exomecnv') }}" + if: "${{ github.event_name != 'push' || (github.event_name == 'push' && github.repository == 'nf-cmgg/exomecnv') }}" runs-on: ubuntu-latest strategy: matrix: @@ -28,13 +28,16 @@ jobs: - "latest-everything" steps: - name: Check out pipeline code - uses: actions/checkout@v4 + uses: actions/checkout@b4ffde65f46336ab88eb53be808477a3936bae11 # v4 - name: Install Nextflow uses: nf-core/setup-nextflow@v1 with: version: "${{ matrix.NXF_VER }}" + - name: Disk space cleanup + uses: jlumbroso/free-disk-space@54081f138730dfa15788a46383842cd2f914a1be # v1.3.1 + - name: Run pipeline with test data # TODO nf-core: You can customise CI pipeline run tests as required # For example: adding multiple test runs with different parameters diff --git a/.github/workflows/clean-up.yml b/.github/workflows/clean-up.yml index 694e90e..0b6b1f2 100644 --- a/.github/workflows/clean-up.yml +++ b/.github/workflows/clean-up.yml @@ -10,7 +10,7 @@ jobs: issues: write pull-requests: write steps: - - uses: actions/stale@v7 + - uses: actions/stale@28ca1036281a5e5922ead5184a1bbf96e5fc984e # v9 with: stale-issue-message: "This issue has been tagged as awaiting-changes or awaiting-feedback by an nf-core contributor. Remove stale label or add a comment otherwise this issue will be closed in 20 days." stale-pr-message: "This PR has been tagged as awaiting-changes or awaiting-feedback by an nf-core contributor. Remove stale label or add a comment if it is still useful." diff --git a/.github/workflows/download_pipeline.yml b/.github/workflows/download_pipeline.yml new file mode 100644 index 0000000..08622fd --- /dev/null +++ b/.github/workflows/download_pipeline.yml @@ -0,0 +1,72 @@ +name: Test successful pipeline download with 'nf-core download' + +# Run the workflow when: +# - dispatched manually +# - when a PR is opened or reopened to master branch +# - the head branch of the pull request is updated, i.e. if fixes for a release are pushed last minute to dev. +on: + workflow_dispatch: + inputs: + testbranch: + description: "The specific branch you wish to utilize for the test execution of nf-core download." + required: true + default: "dev" + pull_request: + types: + - opened + branches: + - master + pull_request_target: + branches: + - master + +env: + NXF_ANSI_LOG: false + +jobs: + download: + runs-on: ubuntu-latest + steps: + - name: Install Nextflow + uses: nf-core/setup-nextflow@v1 + + - uses: actions/setup-python@0a5c61591373683505ea898e09a3ea4f39ef2b9c # v5 + with: + python-version: "3.11" + architecture: "x64" + - uses: eWaterCycle/setup-singularity@931d4e31109e875b13309ae1d07c70ca8fbc8537 # v7 + with: + singularity-version: 3.8.3 + + - name: Install dependencies + run: | + python -m pip install --upgrade pip + pip install git+https://github.com/nf-core/tools.git@dev + + - name: Get the repository name and current branch set as environment variable + run: | + echo "REPO_LOWERCASE=${GITHUB_REPOSITORY,,}" >> ${GITHUB_ENV} + echo "REPOTITLE_LOWERCASE=$(basename ${GITHUB_REPOSITORY,,})" >> ${GITHUB_ENV} + echo "REPO_BRANCH=${{ github.event.inputs.testbranch || 'dev' }}" >> ${GITHUB_ENV} + + - name: Download the pipeline + env: + NXF_SINGULARITY_CACHEDIR: ./ + run: | + nf-core download ${{ env.REPO_LOWERCASE }} \ + --revision ${{ env.REPO_BRANCH }} \ + --outdir ./${{ env.REPOTITLE_LOWERCASE }} \ + --compress "none" \ + --container-system 'singularity' \ + --container-library "quay.io" -l "docker.io" -l "ghcr.io" \ + --container-cache-utilisation 'amend' \ + --download-configuration + + - name: Inspect download + run: tree ./${{ env.REPOTITLE_LOWERCASE }} + + - name: Run the downloaded pipeline + env: + NXF_SINGULARITY_CACHEDIR: ./ + NXF_SINGULARITY_HOME_MOUNT: true + run: nextflow run ./${{ env.REPOTITLE_LOWERCASE }}/$( sed 's/\W/_/g' <<< ${{ env.REPO_BRANCH }}) -stub -profile test,singularity --outdir ./results diff --git a/.github/workflows/fix-linting.yml b/.github/workflows/fix-linting.yml index 3543ad2..a093ccb 100644 --- a/.github/workflows/fix-linting.yml +++ b/.github/workflows/fix-linting.yml @@ -4,19 +4,26 @@ on: types: [created] jobs: - deploy: + fix-linting: # Only run if comment is on a PR with the main repo, and if it contains the magic keywords if: > contains(github.event.comment.html_url, '/pull/') && contains(github.event.comment.body, '@nf-core-bot fix linting') && - github.repository == 'CenterForMedicalGeneticsGhent/nf-cmgg-exomecnv' + github.repository == 'nf-cmgg/exomecnv' runs-on: ubuntu-latest steps: # Use the @nf-core-bot token to check out so we can push later - - uses: actions/checkout@v4 + - uses: actions/checkout@b4ffde65f46336ab88eb53be808477a3936bae11 # v4 with: token: ${{ secrets.nf_core_bot_auth_token }} + # indication that the linting is being fixed + - name: React on comment + uses: peter-evans/create-or-update-comment@71345be0265236311c031f5c7866368bd1eff043 # v4 + with: + comment-id: ${{ github.event.comment.id }} + reactions: eyes + # Action runs on the issue comment, so we don't get the PR by default # Use the gh cli to check out the PR - name: Checkout Pull Request @@ -24,32 +31,59 @@ jobs: env: GITHUB_TOKEN: ${{ secrets.nf_core_bot_auth_token }} - - uses: actions/setup-node@v4 + # Install and run pre-commit + - uses: actions/setup-python@0a5c61591373683505ea898e09a3ea4f39ef2b9c # v5 + with: + python-version: 3.11 - - name: Install Prettier - run: npm install -g prettier @prettier/plugin-php + - name: Install pre-commit + run: pip install pre-commit - # Check that we actually need to fix something - - name: Run 'prettier --check' - id: prettier_status - run: | - if prettier --check ${GITHUB_WORKSPACE}; then - echo "result=pass" >> $GITHUB_OUTPUT - else - echo "result=fail" >> $GITHUB_OUTPUT - fi + - name: Run pre-commit + id: pre-commit + run: pre-commit run --all-files + continue-on-error: true - - name: Run 'prettier --write' - if: steps.prettier_status.outputs.result == 'fail' - run: prettier --write ${GITHUB_WORKSPACE} + # indication that the linting has finished + - name: react if linting finished succesfully + if: steps.pre-commit.outcome == 'success' + uses: peter-evans/create-or-update-comment@71345be0265236311c031f5c7866368bd1eff043 # v4 + with: + comment-id: ${{ github.event.comment.id }} + reactions: "+1" - name: Commit & push changes - if: steps.prettier_status.outputs.result == 'fail' + id: commit-and-push + if: steps.pre-commit.outcome == 'failure' run: | git config user.email "core@nf-co.re" git config user.name "nf-core-bot" git config push.default upstream git add . git status - git commit -m "[automated] Fix linting with Prettier" + git commit -m "[automated] Fix code linting" git push + + - name: react if linting errors were fixed + id: react-if-fixed + if: steps.commit-and-push.outcome == 'success' + uses: peter-evans/create-or-update-comment@71345be0265236311c031f5c7866368bd1eff043 # v4 + with: + comment-id: ${{ github.event.comment.id }} + reactions: hooray + + - name: react if linting errors were not fixed + if: steps.commit-and-push.outcome == 'failure' + uses: peter-evans/create-or-update-comment@71345be0265236311c031f5c7866368bd1eff043 # v4 + with: + comment-id: ${{ github.event.comment.id }} + reactions: confused + + - name: react if linting errors were not fixed + if: steps.commit-and-push.outcome == 'failure' + uses: peter-evans/create-or-update-comment@71345be0265236311c031f5c7866368bd1eff043 # v4 + with: + issue-number: ${{ github.event.issue.number }} + body: | + @${{ github.actor }} I tried to fix the linting errors, but it didn't work. Please fix them manually. + See [CI log](https://github.com/nf-cmgg/exomecnv/actions/runs/${{ github.run_id }}) for more details. diff --git a/.github/workflows/linting.yml b/.github/workflows/linting.yml index 5079018..073e187 100644 --- a/.github/workflows/linting.yml +++ b/.github/workflows/linting.yml @@ -11,74 +11,35 @@ on: types: [published] jobs: - EditorConfig: + pre-commit: runs-on: ubuntu-latest steps: - - uses: actions/checkout@v4 + - uses: actions/checkout@b4ffde65f46336ab88eb53be808477a3936bae11 # v4 - - uses: actions/setup-node@v4 - - - name: Install editorconfig-checker - run: npm install -g editorconfig-checker - - - name: Run ECLint check - run: editorconfig-checker -exclude README.md $(find .* -type f | grep -v '.git\|.py\|.md\|json\|yml\|yaml\|html\|css\|work\|.nextflow\|build\|nf_core.egg-info\|log.txt\|Makefile') - - Prettier: - runs-on: ubuntu-latest - steps: - - uses: actions/checkout@v4 - - - uses: actions/setup-node@v4 - - - name: Install Prettier - run: npm install -g prettier - - - name: Run Prettier --check - run: prettier --check ${GITHUB_WORKSPACE} - - PythonBlack: - runs-on: ubuntu-latest - steps: - - uses: actions/checkout@v4 - - - name: Check code lints with Black - uses: psf/black@stable - - # If the above check failed, post a comment on the PR explaining the failure - - name: Post PR comment - if: failure() - uses: mshick/add-pr-comment@v1 + - name: Set up Python 3.11 + uses: actions/setup-python@0a5c61591373683505ea898e09a3ea4f39ef2b9c # v5 with: - message: | - ## Python linting (`black`) is failing - - To keep the code consistent with lots of contributors, we run automated code consistency checks. - To fix this CI test, please run: - - * Install [`black`](https://black.readthedocs.io/en/stable/): `pip install black` - * Fix formatting errors in your pipeline: `black .` - - Once you push these changes the test should pass, and you can hide this comment :+1: + python-version: 3.11 + cache: "pip" - We highly recommend setting up Black in your code editor so that this formatting is done automatically on save. Ask about it on Slack for help! + - name: Install pre-commit + run: pip install pre-commit - Thanks again for your contribution! - repo-token: ${{ secrets.GITHUB_TOKEN }} - allow-repeats: false + - name: Run pre-commit + run: pre-commit run --all-files nf-core: runs-on: ubuntu-latest steps: - name: Check out pipeline code - uses: actions/checkout@v4 + uses: actions/checkout@b4ffde65f46336ab88eb53be808477a3936bae11 # v4 - name: Install Nextflow uses: nf-core/setup-nextflow@v1 - - uses: actions/setup-python@v4 + - uses: actions/setup-python@0a5c61591373683505ea898e09a3ea4f39ef2b9c # v5 with: - python-version: "3.12" + python-version: "3.11" architecture: "x64" - name: Install dependencies @@ -99,7 +60,7 @@ jobs: - name: Upload linting log file artifact if: ${{ always() }} - uses: actions/upload-artifact@v3 + uses: actions/upload-artifact@5d5d22a31266ced268874388b861e4b58bb5c2f3 # v4 with: name: linting-logs path: | diff --git a/.github/workflows/linting_comment.yml b/.github/workflows/linting_comment.yml index 0bbcd30..b706875 100644 --- a/.github/workflows/linting_comment.yml +++ b/.github/workflows/linting_comment.yml @@ -11,7 +11,7 @@ jobs: runs-on: ubuntu-latest steps: - name: Download lint results - uses: dawidd6/action-download-artifact@v2 + uses: dawidd6/action-download-artifact@f6b0bace624032e30a85a8fd9c1a7f8f611f5737 # v3 with: workflow: linting.yml workflow_conclusion: completed @@ -21,7 +21,7 @@ jobs: run: echo "pr_number=$(cat linting-logs/PR_number.txt)" >> $GITHUB_OUTPUT - name: Post PR comment - uses: marocchino/sticky-pull-request-comment@v2 + uses: marocchino/sticky-pull-request-comment@331f8f5b4215f0445d3c07b4967662a32a2d3e31 # v2 with: GITHUB_TOKEN: ${{ secrets.GITHUB_TOKEN }} number: ${{ steps.pr_number.outputs.pr_number }} diff --git a/.github/workflows/release-announcments.yml b/.github/workflows/release-announcements.yml similarity index 80% rename from .github/workflows/release-announcments.yml rename to .github/workflows/release-announcements.yml index 6ad3392..d468aea 100644 --- a/.github/workflows/release-announcments.yml +++ b/.github/workflows/release-announcements.yml @@ -9,6 +9,11 @@ jobs: toot: runs-on: ubuntu-latest steps: + - name: get topics and convert to hashtags + id: get_topics + run: | + curl -s https://nf-co.re/pipelines.json | jq -r '.remote_workflows[] | select(.full_name == "${{ github.repository }}") | .topics[]' | awk '{print "#"$0}' | tr '\n' ' ' >> $GITHUB_OUTPUT + - uses: rzr/fediverse-action@master with: access-token: ${{ secrets.MASTODON_ACCESS_TOKEN }} @@ -20,11 +25,13 @@ jobs: Please see the changelog: ${{ github.event.release.html_url }} + ${{ steps.get_topics.outputs.GITHUB_OUTPUT }} #nfcore #openscience #nextflow #bioinformatics + send-tweet: runs-on: ubuntu-latest steps: - - uses: actions/setup-python@v4 + - uses: actions/setup-python@0a5c61591373683505ea898e09a3ea4f39ef2b9c # v5 with: python-version: "3.10" - name: Install dependencies @@ -56,7 +63,7 @@ jobs: bsky-post: runs-on: ubuntu-latest steps: - - uses: zentered/bluesky-post-action@v0.0.2 + - uses: zentered/bluesky-post-action@80dbe0a7697de18c15ad22f4619919ceb5ccf597 # v0.1.0 with: post: | Pipeline release! ${{ github.repository }} v${{ github.event.release.tag_name }} - ${{ github.event.release.name }}! diff --git a/.gitpod.yml b/.gitpod.yml index 25488dc..105a182 100644 --- a/.gitpod.yml +++ b/.gitpod.yml @@ -4,16 +4,17 @@ tasks: command: | pre-commit install --install-hooks nextflow self-update + - name: unset JAVA_TOOL_OPTIONS + command: | + unset JAVA_TOOL_OPTIONS vscode: extensions: # based on nf-core.nf-core-extensionpack - - codezombiech.gitignore # Language support for .gitignore files - # - cssho.vscode-svgviewer # SVG viewer - esbenp.prettier-vscode # Markdown/CommonMark linting and style checking for Visual Studio Code - - eamodio.gitlens # Quickly glimpse into whom, why, and when a line or code block was changed - EditorConfig.EditorConfig # override user/workspace settings with settings found in .editorconfig files - Gruntfuggly.todo-tree # Display TODO and FIXME in a tree view in the activity bar - mechatroner.rainbow-csv # Highlight columns in csv files in different colors - # - nextflow.nextflow # Nextflow syntax highlighting + # - nextflow.nextflow # Nextflow syntax highlighting - oderwat.indent-rainbow # Highlight indentation level - streetsidesoftware.code-spell-checker # Spelling checker for source code + - charliermarsh.ruff # Code linter Ruff diff --git a/.nf-core.yml b/.nf-core.yml index bfcaa49..4124271 100644 --- a/.nf-core.yml +++ b/.nf-core.yml @@ -1,26 +1,26 @@ lint: files_exist: - - CODE_OF_CONDUCT.md - - assets/nf-core-nf-cmgg-exomecnv_logo_light.png - - docs/images/nf-core-nf-cmgg-exomecnv_logo_light.png - - docs/images/nf-core-nf-cmgg-exomecnv_logo_dark.png - - .github/ISSUE_TEMPLATE/config.yml - - .github/workflows/awstest.yml - - .github/workflows/awsfulltest.yml + - CODE_OF_CONDUCT.md + - assets/nf-core-exomecnv_logo_light.png + - docs/images/nf-core-exomecnv_logo_light.png + - docs/images/nf-core-exomecnv_logo_dark.png + - .github/ISSUE_TEMPLATE/config.yml + - .github/workflows/awstest.yml + - .github/workflows/awsfulltest.yml files_unchanged: - - CODE_OF_CONDUCT.md - - assets/nf-core-nf-cmgg-exomecnv_logo_light.png - - docs/images/nf-core-nf-cmgg-exomecnv_logo_light.png - - docs/images/nf-core-nf-cmgg-exomecnv_logo_dark.png - - .github/ISSUE_TEMPLATE/bug_report.yml + - CODE_OF_CONDUCT.md + - assets/nf-core-exomecnv_logo_light.png + - docs/images/nf-core-exomecnv_logo_light.png + - docs/images/nf-core-exomecnv_logo_dark.png + - .github/ISSUE_TEMPLATE/bug_report.yml multiqc_config: - - report_comment + - report_comment nextflow_config: - - manifest.name - - manifest.homePage + - manifest.name + - manifest.homePage repository_type: pipeline template: author: nvnieuwk description: A nextflow pipeline for calling exome CNVs - name: nf-cmgg-exomecnv - prefix: CenterForMedicalGeneticsGhent + name: exomecnv + prefix: nf-cmgg diff --git a/.pre-commit-config.yaml b/.pre-commit-config.yaml index 0c31cdb..af57081 100644 --- a/.pre-commit-config.yaml +++ b/.pre-commit-config.yaml @@ -1,5 +1,10 @@ repos: - repo: https://github.com/pre-commit/mirrors-prettier - rev: "v2.7.1" + rev: "v3.1.0" hooks: - id: prettier + - repo: https://github.com/editorconfig-checker/editorconfig-checker.python + rev: "2.7.3" + hooks: + - id: editorconfig-checker + alias: ec diff --git a/CHANGELOG.md b/CHANGELOG.md index 4291585..8b4d42a 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -1,11 +1,11 @@ -# CenterForMedicalGeneticsGhent/nf-cmgg-exomecnv: Changelog +# nf-cmgg/exomecnv: Changelog The format is based on [Keep a Changelog](https://keepachangelog.com/en/1.0.0/) and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0.html). ## v1.0dev - [date] -Initial release of CenterForMedicalGeneticsGhent/nf-cmgg-exomecnv, created with the [nf-core](https://nf-co.re/) template. +Initial release of nf-cmgg/exomecnv, created with the [nf-core](https://nf-co.re/) template. ### `Added` diff --git a/CITATIONS.md b/CITATIONS.md index 70fc7d8..b2570fc 100644 --- a/CITATIONS.md +++ b/CITATIONS.md @@ -1,4 +1,4 @@ -# CenterForMedicalGeneticsGhent/nf-cmgg-exomecnv: Citations +# nf-cmgg/exomecnv: Citations ## [nf-core](https://pubmed.ncbi.nlm.nih.gov/32055031/) diff --git a/README.md b/README.md index 40255f9..c178e93 100644 --- a/README.md +++ b/README.md @@ -1,15 +1,16 @@ -[![GitHub Actions CI Status](https://github.com/CenterForMedicalGeneticsGhent/nf-cmgg-exomecnv/workflows/nf-core%20CI/badge.svg)](https://github.com/CenterForMedicalGeneticsGhent/nf-cmgg-exomecnv/actions?query=workflow%3A%22nf-core+CI%22) -[![GitHub Actions Linting Status](https://github.com/CenterForMedicalGeneticsGhent/nf-cmgg-exomecnv/workflows/nf-core%20linting/badge.svg)](https://github.com/CenterForMedicalGeneticsGhent/nf-cmgg-exomecnv/actions?query=workflow%3A%22nf-core+linting%22)[![Cite with Zenodo](http://img.shields.io/badge/DOI-10.5281/zenodo.XXXXXXX-1073c8?labelColor=000000)](https://doi.org/10.5281/zenodo.XXXXXXX) +[![GitHub Actions CI Status](https://github.com/nf-cmgg/exomecnv/actions/workflows/ci.yml/badge.svg)](https://github.com/nf-cmgg/exomecnv/actions/workflows/ci.yml) +[![GitHub Actions Linting Status](https://github.com/nf-cmgg/exomecnv/actions/workflows/linting.yml/badge.svg)](https://github.com/nf-cmgg/exomecnv/actions/workflows/linting.yml)[![Cite with Zenodo](http://img.shields.io/badge/DOI-10.5281/zenodo.XXXXXXX-1073c8?labelColor=000000)](https://doi.org/10.5281/zenodo.XXXXXXX) +[![nf-test](https://img.shields.io/badge/unit_tests-nf--test-337ab7.svg)](https://www.nf-test.com) [![Nextflow](https://img.shields.io/badge/nextflow%20DSL2-%E2%89%A523.04.0-23aa62.svg)](https://www.nextflow.io/) [![run with conda](http://img.shields.io/badge/run%20with-conda-3EB049?labelColor=000000&logo=anaconda)](https://docs.conda.io/en/latest/) [![run with docker](https://img.shields.io/badge/run%20with-docker-0db7ed?labelColor=000000&logo=docker)](https://www.docker.com/) [![run with singularity](https://img.shields.io/badge/run%20with-singularity-1d355c.svg?labelColor=000000)](https://sylabs.io/docs/) -[![Launch on Nextflow Tower](https://img.shields.io/badge/Launch%20%F0%9F%9A%80-Nextflow%20Tower-%234256e7)](https://tower.nf/launch?pipeline=https://github.com/CenterForMedicalGeneticsGhent/nf-cmgg-exomecnv) +[![Launch on Seqera Platform](https://img.shields.io/badge/Launch%20%F0%9F%9A%80-Seqera%20Platform-%234256e7)](https://tower.nf/launch?pipeline=https://github.com/nf-cmgg/exomecnv) ## Introduction -**CenterForMedicalGeneticsGhent/nf-cmgg-exomecnv** is a bioinformatics pipeline that ... +**nf-cmgg/exomecnv** is a bioinformatics pipeline that ... ```bash -nextflow run CenterForMedicalGeneticsGhent/nf-cmgg-exomecnv \ +nextflow run nf-cmgg/exomecnv \ -profile \ --input samplesheet.csv \ --outdir ``` -:::warning -Please provide pipeline parameters via the CLI or Nextflow `-params-file` option. Custom config files including those -provided by the `-c` Nextflow option can be used to provide any configuration _**except for parameters**_; -see [docs](https://nf-co.re/usage/configuration#custom-configuration-files). -::: +> [!WARNING] +> Please provide pipeline parameters via the CLI or Nextflow `-params-file` option. Custom config files including those provided by the `-c` Nextflow option can be used to provide any configuration _**except for parameters**_; +> see [docs](https://nf-co.re/usage/configuration#custom-configuration-files). ## Credits -CenterForMedicalGeneticsGhent/nf-cmgg-exomecnv was originally written by nvnieuwk. +nf-cmgg/exomecnv was originally written by nvnieuwk. We thank the following people for their extensive assistance in the development of this pipeline: @@ -80,7 +76,7 @@ If you would like to contribute to this pipeline, please see the [contributing g ## Citations - + diff --git a/assets/adaptivecard.json b/assets/adaptivecard.json index f7dc263..24298f2 100644 --- a/assets/adaptivecard.json +++ b/assets/adaptivecard.json @@ -17,7 +17,7 @@ "size": "Large", "weight": "Bolder", "color": "<% if (success) { %>Good<% } else { %>Attention<%} %>", - "text": "CenterForMedicalGeneticsGhent/nf-cmgg-exomecnv v${version} - ${runName}", + "text": "nf-cmgg/exomecnv v${version} - ${runName}", "wrap": true }, { diff --git a/assets/email_template.html b/assets/email_template.html index aa7ac63..64f9c19 100644 --- a/assets/email_template.html +++ b/assets/email_template.html @@ -4,21 +4,21 @@ - - CenterForMedicalGeneticsGhent/nf-cmgg-exomecnv Pipeline Report + + nf-cmgg/exomecnv Pipeline Report
-

CenterForMedicalGeneticsGhent/nf-cmgg-exomecnv v${version}

+

nf-cmgg/exomecnv ${version}

Run Name: $runName

<% if (!success){ out << """
-

CenterForMedicalGeneticsGhent/nf-cmgg-exomecnv execution completed unsuccessfully!

+

nf-cmgg/exomecnv execution completed unsuccessfully!

The exit status of the task that caused the workflow execution to fail was: $exitStatus.

The full error message was:

${errorReport}
@@ -27,7 +27,7 @@

CenterForMedicalGeneticsGhent/nf-cmgg- } else { out << """
- CenterForMedicalGeneticsGhent/nf-cmgg-exomecnv execution completed successfully! + nf-cmgg/exomecnv execution completed successfully!
""" } @@ -44,8 +44,8 @@

Pipeline Configuration:

-

CenterForMedicalGeneticsGhent/nf-cmgg-exomecnv

-

https://github.com/CenterForMedicalGeneticsGhent/nf-cmgg-exomecnv

+

nf-cmgg/exomecnv

+

https://github.com/nf-cmgg/exomecnv

diff --git a/assets/email_template.txt b/assets/email_template.txt index 247ba11..92d7395 100644 --- a/assets/email_template.txt +++ b/assets/email_template.txt @@ -1,10 +1,10 @@ Run Name: $runName <% if (success){ - out << "## CenterForMedicalGeneticsGhent/nf-cmgg-exomecnv execution completed successfully! ##" + out << "## nf-cmgg/exomecnv execution completed successfully! ##" } else { out << """#################################################### -## CenterForMedicalGeneticsGhent/nf-cmgg-exomecnv execution completed unsuccessfully! ## +## nf-cmgg/exomecnv execution completed unsuccessfully! ## #################################################### The exit status of the task that caused the workflow execution to fail was: $exitStatus. The full error message was: @@ -27,5 +27,5 @@ Pipeline Configuration: <% out << summary.collect{ k,v -> " - $k: $v" }.join("\n") %> -- -CenterForMedicalGeneticsGhent/nf-cmgg-exomecnv -https://github.com/CenterForMedicalGeneticsGhent/nf-cmgg-exomecnv +nf-cmgg/exomecnv +https://github.com/nf-cmgg/exomecnv diff --git a/assets/methods_description_template.yml b/assets/methods_description_template.yml index 79802a0..8cac086 100644 --- a/assets/methods_description_template.yml +++ b/assets/methods_description_template.yml @@ -1,13 +1,13 @@ -id: "CenterForMedicalGeneticsGhent-nf-cmgg-exomecnv-methods-description" +id: "nf-cmgg-exomecnv-methods-description" description: "Suggested text and references to use when describing pipeline usage within the methods section of a publication." -section_name: "CenterForMedicalGeneticsGhent/nf-cmgg-exomecnv Methods Description" -section_href: "https://github.com/CenterForMedicalGeneticsGhent/nf-cmgg-exomecnv" +section_name: "nf-cmgg/exomecnv Methods Description" +section_href: "https://github.com/nf-cmgg/exomecnv" plot_type: "html" ## TODO nf-core: Update the HTML below to your preferred methods description, e.g. add publication citation for this pipeline ## You inject any metadata in the Nextflow '${workflow}' object data: |

Methods

-

Data was processed using CenterForMedicalGeneticsGhent/nf-cmgg-exomecnv v${workflow.manifest.version} ${doi_text} of the nf-core collection of workflows (Ewels et al., 2020), utilising reproducible software environments from the Bioconda (Grüning et al., 2018) and Biocontainers (da Veiga Leprevost et al., 2017) projects.

+

Data was processed using nf-cmgg/exomecnv v${workflow.manifest.version} ${doi_text} of the nf-core collection of workflows (Ewels et al., 2020), utilising reproducible software environments from the Bioconda (Grüning et al., 2018) and Biocontainers (da Veiga Leprevost et al., 2017) projects.

The pipeline was executed with Nextflow v${workflow.nextflow.version} (Di Tommaso et al., 2017) with the following command:

${workflow.commandLine}

${tool_citations}

diff --git a/assets/multiqc_config.yml b/assets/multiqc_config.yml index 287b84b..5269074 100644 --- a/assets/multiqc_config.yml +++ b/assets/multiqc_config.yml @@ -1,12 +1,14 @@ report_comment: > - This report has been generated by the CenterForMedicalGeneticsGhent/nf-cmgg-exomecnv + This report has been generated by the nf-cmgg/exomecnv analysis pipeline. report_section_order: - "CenterForMedicalGeneticsGhent-nf-cmgg-exomecnv-methods-description": + "nf-cmgg-exomecnv-methods-description": order: -1000 software_versions: order: -1001 - "CenterForMedicalGeneticsGhent-nf-cmgg-exomecnv-summary": + "nf-cmgg-exomecnv-summary": order: -1002 export_plots: true + +disable_version_detection: true diff --git a/assets/schema_input.json b/assets/schema_input.json index c84558d..c3e15f0 100644 --- a/assets/schema_input.json +++ b/assets/schema_input.json @@ -1,7 +1,7 @@ { "$schema": "http://json-schema.org/draft-07/schema", - "$id": "https://raw.githubusercontent.com/CenterForMedicalGeneticsGhent/nf-cmgg-exomecnv/master/assets/schema_input.json", - "title": "CenterForMedicalGeneticsGhent/nf-cmgg-exomecnv pipeline - params.input schema", + "$id": "https://raw.githubusercontent.com/nf-cmgg/exomecnv/master/assets/schema_input.json", + "title": "nf-cmgg/exomecnv pipeline - params.input schema", "description": "Schema for the file provided with params.input", "type": "array", "items": { @@ -10,25 +10,22 @@ "sample": { "type": "string", "pattern": "^\\S+$", - "errorMessage": "Sample name must be provided and cannot contain spaces" + "errorMessage": "Sample name must be provided and cannot contain spaces", + "meta": ["id"] }, "fastq_1": { "type": "string", + "format": "file-path", + "exists": true, "pattern": "^\\S+\\.f(ast)?q\\.gz$", "errorMessage": "FastQ file for reads 1 must be provided, cannot contain spaces and must have extension '.fq.gz' or '.fastq.gz'" }, "fastq_2": { - "errorMessage": "FastQ file for reads 2 cannot contain spaces and must have extension '.fq.gz' or '.fastq.gz'", - "anyOf": [ - { - "type": "string", - "pattern": "^\\S+\\.f(ast)?q\\.gz$" - }, - { - "type": "string", - "maxLength": 0 - } - ] + "type": "string", + "format": "file-path", + "exists": true, + "pattern": "^\\S+\\.f(ast)?q\\.gz$", + "errorMessage": "FastQ file for reads 2 cannot contain spaces and must have extension '.fq.gz' or '.fastq.gz'" } }, "required": ["sample", "fastq_1"] diff --git a/assets/sendmail_template.txt b/assets/sendmail_template.txt index 4f0cc10..f701752 100644 --- a/assets/sendmail_template.txt +++ b/assets/sendmail_template.txt @@ -9,12 +9,12 @@ Content-Type: text/html; charset=utf-8 $email_html --nfcoremimeboundary -Content-Type: image/png;name="CenterForMedicalGeneticsGhent-nf-cmgg-exomecnv_logo.png" +Content-Type: image/png;name="nf-cmgg-exomecnv_logo.png" Content-Transfer-Encoding: base64 Content-ID: -Content-Disposition: inline; filename="CenterForMedicalGeneticsGhent-nf-cmgg-exomecnv_logo_light.png" +Content-Disposition: inline; filename="nf-cmgg-exomecnv_logo_light.png" -<% out << new File("$projectDir/assets/CenterForMedicalGeneticsGhent-nf-cmgg-exomecnv_logo_light.png"). +<% out << new File("$projectDir/assets/nf-cmgg-exomecnv_logo_light.png"). bytes. encodeBase64(). toString(). diff --git a/assets/slackreport.json b/assets/slackreport.json index dd93025..84aba38 100644 --- a/assets/slackreport.json +++ b/assets/slackreport.json @@ -3,7 +3,7 @@ { "fallback": "Plain-text summary of the attachment.", "color": "<% if (success) { %>good<% } else { %>danger<%} %>", - "author_name": "CenterForMedicalGeneticsGhent/nf-cmgg-exomecnv ${version} - ${runName}", + "author_name": "nf-cmgg/exomecnv ${version} - ${runName}", "author_icon": "https://www.nextflow.io/docs/latest/_static/favicon.ico", "text": "<% if (success) { %>Pipeline completed successfully!<% } else { %>Pipeline completed with errors<% } %>", "fields": [ diff --git a/bin/check_samplesheet.py b/bin/check_samplesheet.py deleted file mode 100755 index 4a758fe..0000000 --- a/bin/check_samplesheet.py +++ /dev/null @@ -1,259 +0,0 @@ -#!/usr/bin/env python - - -"""Provide a command line tool to validate and transform tabular samplesheets.""" - - -import argparse -import csv -import logging -import sys -from collections import Counter -from pathlib import Path - -logger = logging.getLogger() - - -class RowChecker: - """ - Define a service that can validate and transform each given row. - - Attributes: - modified (list): A list of dicts, where each dict corresponds to a previously - validated and transformed row. The order of rows is maintained. - - """ - - VALID_FORMATS = ( - ".fq.gz", - ".fastq.gz", - ) - - def __init__( - self, - sample_col="sample", - first_col="fastq_1", - second_col="fastq_2", - single_col="single_end", - **kwargs, - ): - """ - Initialize the row checker with the expected column names. - - Args: - sample_col (str): The name of the column that contains the sample name - (default "sample"). - first_col (str): The name of the column that contains the first (or only) - FASTQ file path (default "fastq_1"). - second_col (str): The name of the column that contains the second (if any) - FASTQ file path (default "fastq_2"). - single_col (str): The name of the new column that will be inserted and - records whether the sample contains single- or paired-end sequencing - reads (default "single_end"). - - """ - super().__init__(**kwargs) - self._sample_col = sample_col - self._first_col = first_col - self._second_col = second_col - self._single_col = single_col - self._seen = set() - self.modified = [] - - def validate_and_transform(self, row): - """ - Perform all validations on the given row and insert the read pairing status. - - Args: - row (dict): A mapping from column headers (keys) to elements of that row - (values). - - """ - self._validate_sample(row) - self._validate_first(row) - self._validate_second(row) - self._validate_pair(row) - self._seen.add((row[self._sample_col], row[self._first_col])) - self.modified.append(row) - - def _validate_sample(self, row): - """Assert that the sample name exists and convert spaces to underscores.""" - if len(row[self._sample_col]) <= 0: - raise AssertionError("Sample input is required.") - # Sanitize samples slightly. - row[self._sample_col] = row[self._sample_col].replace(" ", "_") - - def _validate_first(self, row): - """Assert that the first FASTQ entry is non-empty and has the right format.""" - if len(row[self._first_col]) <= 0: - raise AssertionError("At least the first FASTQ file is required.") - self._validate_fastq_format(row[self._first_col]) - - def _validate_second(self, row): - """Assert that the second FASTQ entry has the right format if it exists.""" - if len(row[self._second_col]) > 0: - self._validate_fastq_format(row[self._second_col]) - - def _validate_pair(self, row): - """Assert that read pairs have the same file extension. Report pair status.""" - if row[self._first_col] and row[self._second_col]: - row[self._single_col] = False - first_col_suffix = Path(row[self._first_col]).suffixes[-2:] - second_col_suffix = Path(row[self._second_col]).suffixes[-2:] - if first_col_suffix != second_col_suffix: - raise AssertionError("FASTQ pairs must have the same file extensions.") - else: - row[self._single_col] = True - - def _validate_fastq_format(self, filename): - """Assert that a given filename has one of the expected FASTQ extensions.""" - if not any(filename.endswith(extension) for extension in self.VALID_FORMATS): - raise AssertionError( - f"The FASTQ file has an unrecognized extension: {filename}\n" - f"It should be one of: {', '.join(self.VALID_FORMATS)}" - ) - - def validate_unique_samples(self): - """ - Assert that the combination of sample name and FASTQ filename is unique. - - In addition to the validation, also rename all samples to have a suffix of _T{n}, where n is the - number of times the same sample exist, but with different FASTQ files, e.g., multiple runs per experiment. - - """ - if len(self._seen) != len(self.modified): - raise AssertionError("The pair of sample name and FASTQ must be unique.") - seen = Counter() - for row in self.modified: - sample = row[self._sample_col] - seen[sample] += 1 - row[self._sample_col] = f"{sample}_T{seen[sample]}" - - -def read_head(handle, num_lines=10): - """Read the specified number of lines from the current position in the file.""" - lines = [] - for idx, line in enumerate(handle): - if idx == num_lines: - break - lines.append(line) - return "".join(lines) - - -def sniff_format(handle): - """ - Detect the tabular format. - - Args: - handle (text file): A handle to a `text file`_ object. The read position is - expected to be at the beginning (index 0). - - Returns: - csv.Dialect: The detected tabular format. - - .. _text file: - https://docs.python.org/3/glossary.html#term-text-file - - """ - peek = read_head(handle) - handle.seek(0) - sniffer = csv.Sniffer() - dialect = sniffer.sniff(peek) - return dialect - - -def check_samplesheet(file_in, file_out): - """ - Check that the tabular samplesheet has the structure expected by nf-core pipelines. - - Validate the general shape of the table, expected columns, and each row. Also add - an additional column which records whether one or two FASTQ reads were found. - - Args: - file_in (pathlib.Path): The given tabular samplesheet. The format can be either - CSV, TSV, or any other format automatically recognized by ``csv.Sniffer``. - file_out (pathlib.Path): Where the validated and transformed samplesheet should - be created; always in CSV format. - - Example: - This function checks that the samplesheet follows the following structure, - see also the `viral recon samplesheet`_:: - - sample,fastq_1,fastq_2 - SAMPLE_PE,SAMPLE_PE_RUN1_1.fastq.gz,SAMPLE_PE_RUN1_2.fastq.gz - SAMPLE_PE,SAMPLE_PE_RUN2_1.fastq.gz,SAMPLE_PE_RUN2_2.fastq.gz - SAMPLE_SE,SAMPLE_SE_RUN1_1.fastq.gz, - - .. _viral recon samplesheet: - https://raw.githubusercontent.com/nf-core/test-datasets/viralrecon/samplesheet/samplesheet_test_illumina_amplicon.csv - - """ - required_columns = {"sample", "fastq_1", "fastq_2"} - # See https://docs.python.org/3.9/library/csv.html#id3 to read up on `newline=""`. - with file_in.open(newline="") as in_handle: - reader = csv.DictReader(in_handle, dialect=sniff_format(in_handle)) - # Validate the existence of the expected header columns. - if not required_columns.issubset(reader.fieldnames): - req_cols = ", ".join(required_columns) - logger.critical(f"The sample sheet **must** contain these column headers: {req_cols}.") - sys.exit(1) - # Validate each row. - checker = RowChecker() - for i, row in enumerate(reader): - try: - checker.validate_and_transform(row) - except AssertionError as error: - logger.critical(f"{str(error)} On line {i + 2}.") - sys.exit(1) - checker.validate_unique_samples() - header = list(reader.fieldnames) - header.insert(1, "single_end") - # See https://docs.python.org/3.9/library/csv.html#id3 to read up on `newline=""`. - with file_out.open(mode="w", newline="") as out_handle: - writer = csv.DictWriter(out_handle, header, delimiter=",") - writer.writeheader() - for row in checker.modified: - writer.writerow(row) - - -def parse_args(argv=None): - """Define and immediately parse command line arguments.""" - parser = argparse.ArgumentParser( - description="Validate and transform a tabular samplesheet.", - epilog="Example: python check_samplesheet.py samplesheet.csv samplesheet.valid.csv", - ) - parser.add_argument( - "file_in", - metavar="FILE_IN", - type=Path, - help="Tabular input samplesheet in CSV or TSV format.", - ) - parser.add_argument( - "file_out", - metavar="FILE_OUT", - type=Path, - help="Transformed output samplesheet in CSV format.", - ) - parser.add_argument( - "-l", - "--log-level", - help="The desired log level (default WARNING).", - choices=("CRITICAL", "ERROR", "WARNING", "INFO", "DEBUG"), - default="WARNING", - ) - return parser.parse_args(argv) - - -def main(argv=None): - """Coordinate argument parsing and program execution.""" - args = parse_args(argv) - logging.basicConfig(level=args.log_level, format="[%(levelname)s] %(message)s") - if not args.file_in.is_file(): - logger.error(f"The given input file {args.file_in} was not found!") - sys.exit(2) - args.file_out.parent.mkdir(parents=True, exist_ok=True) - check_samplesheet(args.file_in, args.file_out) - - -if __name__ == "__main__": - sys.exit(main()) diff --git a/conf/base.config b/conf/base.config index 37f670d..bbae968 100644 --- a/conf/base.config +++ b/conf/base.config @@ -1,6 +1,6 @@ /* ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ - CenterForMedicalGeneticsGhent/nf-cmgg-exomecnv Nextflow base config file + nf-cmgg/exomecnv Nextflow base config file ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ A 'blank slate' config file, appropriate for general use on most high performance compute environments. Assumes that all software is installed and available on diff --git a/conf/modules.config b/conf/modules.config index d91c6ab..e3ea8fa 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -18,14 +18,6 @@ process { saveAs: { filename -> filename.equals('versions.yml') ? null : filename } ] - withName: SAMPLESHEET_CHECK { - publishDir = [ - path: { "${params.outdir}/pipeline_info" }, - mode: params.publish_dir_mode, - saveAs: { filename -> filename.equals('versions.yml') ? null : filename } - ] - } - withName: FASTQC { ext.args = '--quiet' } diff --git a/conf/test.config b/conf/test.config index f1a6b13..893b94c 100644 --- a/conf/test.config +++ b/conf/test.config @@ -5,7 +5,7 @@ Defines input files and everything required to run a fast and simple pipeline test. Use as follows: - nextflow run CenterForMedicalGeneticsGhent/nf-cmgg-exomecnv -profile test, --outdir + nextflow run nf-cmgg/exomecnv -profile test, --outdir ---------------------------------------------------------------------------------------- */ diff --git a/conf/test_full.config b/conf/test_full.config index eb86cdc..46990f4 100644 --- a/conf/test_full.config +++ b/conf/test_full.config @@ -5,7 +5,7 @@ Defines input files and everything required to run a full size pipeline test. Use as follows: - nextflow run CenterForMedicalGeneticsGhent/nf-cmgg-exomecnv -profile test_full, --outdir + nextflow run nf-cmgg/exomecnv -profile test_full, --outdir ---------------------------------------------------------------------------------------- */ diff --git a/docs/README.md b/docs/README.md index d02d01b..2b19b49 100644 --- a/docs/README.md +++ b/docs/README.md @@ -1,6 +1,6 @@ -# CenterForMedicalGeneticsGhent/nf-cmgg-exomecnv: Documentation +# nf-cmgg/exomecnv: Documentation -The CenterForMedicalGeneticsGhent/nf-cmgg-exomecnv documentation is split into the following pages: +The nf-cmgg/exomecnv documentation is split into the following pages: - [Usage](usage.md) - An overview of how the pipeline works, how to run it and a description of all of the different command-line flags. diff --git a/docs/output.md b/docs/output.md index c1f10be..1fa4efd 100644 --- a/docs/output.md +++ b/docs/output.md @@ -1,4 +1,4 @@ -# CenterForMedicalGeneticsGhent/nf-cmgg-exomecnv: Output +# nf-cmgg/exomecnv: Output ## Introduction diff --git a/docs/usage.md b/docs/usage.md index 70cc949..3be8450 100644 --- a/docs/usage.md +++ b/docs/usage.md @@ -1,4 +1,4 @@ -# CenterForMedicalGeneticsGhent/nf-cmgg-exomecnv: Usage +# nf-cmgg/exomecnv: Usage > _Documentation of pipeline parameters is generated automatically from the pipeline schema and can no longer be found in markdown files._ @@ -55,7 +55,7 @@ An [example samplesheet](../assets/samplesheet.csv) has been provided with the p The typical command for running the pipeline is as follows: ```bash -nextflow run CenterForMedicalGeneticsGhent/nf-cmgg-exomecnv --input ./samplesheet.csv --outdir ./results --genome GRCh37 -profile docker +nextflow run nf-cmgg/exomecnv --input ./samplesheet.csv --outdir ./results --genome GRCh37 -profile docker ``` This will launch the pipeline with the `docker` configuration profile. See below for more information about profiles. @@ -80,7 +80,7 @@ Do not use `-c ` to specify parameters as this will result in errors. Cust The above pipeline run specified with a params file in yaml format: ```bash -nextflow run CenterForMedicalGeneticsGhent/nf-cmgg-exomecnv -profile docker -params-file params.yaml +nextflow run nf-cmgg/exomecnv -profile docker -params-file params.yaml ``` with `params.yaml` containing: @@ -99,14 +99,14 @@ You can also generate such `YAML`/`JSON` files via [nf-core/launch](https://nf-c When you run the above command, Nextflow automatically pulls the pipeline code from GitHub and stores it as a cached version. When running the pipeline after this, it will always use the cached version if available - even if the pipeline has been updated since. To make sure that you're running the latest version of the pipeline, make sure that you regularly update the cached version of the pipeline: ```bash -nextflow pull CenterForMedicalGeneticsGhent/nf-cmgg-exomecnv +nextflow pull nf-cmgg/exomecnv ``` ### Reproducibility It is a good idea to specify a pipeline version when running the pipeline on your data. This ensures that a specific version of the pipeline code and software are used when you run your pipeline. If you keep using the same tag, you'll be running the same version of the pipeline, even if there have been changes to the code since. -First, go to the [CenterForMedicalGeneticsGhent/nf-cmgg-exomecnv releases page](https://github.com/CenterForMedicalGeneticsGhent/nf-cmgg-exomecnv/releases) and find the latest pipeline version - numeric only (eg. `1.3.1`). Then specify this when running the pipeline with `-r` (one hyphen) - eg. `-r 1.3.1`. Of course, you can switch to another version by changing the number after the `-r` flag. +First, go to the [nf-cmgg/exomecnv releases page](https://github.com/nf-cmgg/exomecnv/releases) and find the latest pipeline version - numeric only (eg. `1.3.1`). Then specify this when running the pipeline with `-r` (one hyphen) - eg. `-r 1.3.1`. Of course, you can switch to another version by changing the number after the `-r` flag. This version number will be logged in reports when you run the pipeline, so that you'll know what you used when you look back in the future. For example, at the bottom of the MultiQC reports. diff --git a/lib/NfcoreTemplate.groovy b/lib/NfcoreTemplate.groovy deleted file mode 100755 index 4ce590c..0000000 --- a/lib/NfcoreTemplate.groovy +++ /dev/null @@ -1,351 +0,0 @@ -// -// This file holds several functions used within the nf-core pipeline template. -// - -import org.yaml.snakeyaml.Yaml -import groovy.json.JsonOutput -import nextflow.extension.FilesEx - -class NfcoreTemplate { - - // - // Check AWS Batch related parameters have been specified correctly - // - public static void awsBatch(workflow, params) { - if (workflow.profile.contains('awsbatch')) { - // Check params.awsqueue and params.awsregion have been set if running on AWSBatch - assert (params.awsqueue && params.awsregion) : "Specify correct --awsqueue and --awsregion parameters on AWSBatch!" - // Check outdir paths to be S3 buckets if running on AWSBatch - assert params.outdir.startsWith('s3:') : "Outdir not on S3 - specify S3 Bucket to run on AWSBatch!" - } - } - - // - // Warn if a -profile or Nextflow config has not been provided to run the pipeline - // - public static void checkConfigProvided(workflow, log) { - if (workflow.profile == 'standard' && workflow.configFiles.size() <= 1) { - log.warn "[$workflow.manifest.name] You are attempting to run the pipeline without any custom configuration!\n\n" + - "This will be dependent on your local compute environment but can be achieved via one or more of the following:\n" + - " (1) Using an existing pipeline profile e.g. `-profile docker` or `-profile singularity`\n" + - " (2) Using an existing nf-core/configs for your Institution e.g. `-profile crick` or `-profile uppmax`\n" + - " (3) Using your own local custom config e.g. `-c /path/to/your/custom.config`\n\n" + - "Please refer to the quick start section and usage docs for the pipeline.\n " - } - } - - // - // Generate version string - // - public static String version(workflow) { - String version_string = "" - - if (workflow.manifest.version) { - def prefix_v = workflow.manifest.version[0] != 'v' ? 'v' : '' - version_string += "${prefix_v}${workflow.manifest.version}" - } - - if (workflow.commitId) { - def git_shortsha = workflow.commitId.substring(0, 7) - version_string += "-g${git_shortsha}" - } - - return version_string - } - - // - // Construct and send completion email - // - public static void email(workflow, params, summary_params, projectDir, log, multiqc_report=[]) { - - // Set up the e-mail variables - def subject = "[$workflow.manifest.name] Successful: $workflow.runName" - if (!workflow.success) { - subject = "[$workflow.manifest.name] FAILED: $workflow.runName" - } - - def summary = [:] - for (group in summary_params.keySet()) { - summary << summary_params[group] - } - - def misc_fields = [:] - misc_fields['Date Started'] = workflow.start - misc_fields['Date Completed'] = workflow.complete - misc_fields['Pipeline script file path'] = workflow.scriptFile - misc_fields['Pipeline script hash ID'] = workflow.scriptId - if (workflow.repository) misc_fields['Pipeline repository Git URL'] = workflow.repository - if (workflow.commitId) misc_fields['Pipeline repository Git Commit'] = workflow.commitId - if (workflow.revision) misc_fields['Pipeline Git branch/tag'] = workflow.revision - misc_fields['Nextflow Version'] = workflow.nextflow.version - misc_fields['Nextflow Build'] = workflow.nextflow.build - misc_fields['Nextflow Compile Timestamp'] = workflow.nextflow.timestamp - - def email_fields = [:] - email_fields['version'] = NfcoreTemplate.version(workflow) - email_fields['runName'] = workflow.runName - email_fields['success'] = workflow.success - email_fields['dateComplete'] = workflow.complete - email_fields['duration'] = workflow.duration - email_fields['exitStatus'] = workflow.exitStatus - email_fields['errorMessage'] = (workflow.errorMessage ?: 'None') - email_fields['errorReport'] = (workflow.errorReport ?: 'None') - email_fields['commandLine'] = workflow.commandLine - email_fields['projectDir'] = workflow.projectDir - email_fields['summary'] = summary << misc_fields - - // On success try attach the multiqc report - def mqc_report = null - try { - if (workflow.success) { - mqc_report = multiqc_report.getVal() - if (mqc_report.getClass() == ArrayList && mqc_report.size() >= 1) { - if (mqc_report.size() > 1) { - log.warn "[$workflow.manifest.name] Found multiple reports from process 'MULTIQC', will use only one" - } - mqc_report = mqc_report[0] - } - } - } catch (all) { - if (multiqc_report) { - log.warn "[$workflow.manifest.name] Could not attach MultiQC report to summary email" - } - } - - // Check if we are only sending emails on failure - def email_address = params.email - if (!params.email && params.email_on_fail && !workflow.success) { - email_address = params.email_on_fail - } - - // Render the TXT template - def engine = new groovy.text.GStringTemplateEngine() - def tf = new File("$projectDir/assets/email_template.txt") - def txt_template = engine.createTemplate(tf).make(email_fields) - def email_txt = txt_template.toString() - - // Render the HTML template - def hf = new File("$projectDir/assets/email_template.html") - def html_template = engine.createTemplate(hf).make(email_fields) - def email_html = html_template.toString() - - // Render the sendmail template - def max_multiqc_email_size = (params.containsKey('max_multiqc_email_size') ? params.max_multiqc_email_size : 0) as nextflow.util.MemoryUnit - def smail_fields = [ email: email_address, subject: subject, email_txt: email_txt, email_html: email_html, projectDir: "$projectDir", mqcFile: mqc_report, mqcMaxSize: max_multiqc_email_size.toBytes() ] - def sf = new File("$projectDir/assets/sendmail_template.txt") - def sendmail_template = engine.createTemplate(sf).make(smail_fields) - def sendmail_html = sendmail_template.toString() - - // Send the HTML e-mail - Map colors = logColours(params.monochrome_logs) - if (email_address) { - try { - if (params.plaintext_email) { throw GroovyException('Send plaintext e-mail, not HTML') } - // Try to send HTML e-mail using sendmail - def sendmail_tf = new File(workflow.launchDir.toString(), ".sendmail_tmp.html") - sendmail_tf.withWriter { w -> w << sendmail_html } - [ 'sendmail', '-t' ].execute() << sendmail_html - log.info "-${colors.purple}[$workflow.manifest.name]${colors.green} Sent summary e-mail to $email_address (sendmail)-" - } catch (all) { - // Catch failures and try with plaintext - def mail_cmd = [ 'mail', '-s', subject, '--content-type=text/html', email_address ] - if ( mqc_report != null && mqc_report.size() <= max_multiqc_email_size.toBytes() ) { - mail_cmd += [ '-A', mqc_report ] - } - mail_cmd.execute() << email_html - log.info "-${colors.purple}[$workflow.manifest.name]${colors.green} Sent summary e-mail to $email_address (mail)-" - } - } - - // Write summary e-mail HTML to a file - def output_hf = new File(workflow.launchDir.toString(), ".pipeline_report.html") - output_hf.withWriter { w -> w << email_html } - FilesEx.copyTo(output_hf.toPath(), "${params.outdir}/pipeline_info/pipeline_report.html"); - output_hf.delete() - - // Write summary e-mail TXT to a file - def output_tf = new File(workflow.launchDir.toString(), ".pipeline_report.txt") - output_tf.withWriter { w -> w << email_txt } - FilesEx.copyTo(output_tf.toPath(), "${params.outdir}/pipeline_info/pipeline_report.txt"); - output_tf.delete() - } - - // - // Construct and send a notification to a web server as JSON - // e.g. Microsoft Teams and Slack - // - public static void IM_notification(workflow, params, summary_params, projectDir, log) { - def hook_url = params.hook_url - - def summary = [:] - for (group in summary_params.keySet()) { - summary << summary_params[group] - } - - def misc_fields = [:] - misc_fields['start'] = workflow.start - misc_fields['complete'] = workflow.complete - misc_fields['scriptfile'] = workflow.scriptFile - misc_fields['scriptid'] = workflow.scriptId - if (workflow.repository) misc_fields['repository'] = workflow.repository - if (workflow.commitId) misc_fields['commitid'] = workflow.commitId - if (workflow.revision) misc_fields['revision'] = workflow.revision - misc_fields['nxf_version'] = workflow.nextflow.version - misc_fields['nxf_build'] = workflow.nextflow.build - misc_fields['nxf_timestamp'] = workflow.nextflow.timestamp - - def msg_fields = [:] - msg_fields['version'] = NfcoreTemplate.version(workflow) - msg_fields['runName'] = workflow.runName - msg_fields['success'] = workflow.success - msg_fields['dateComplete'] = workflow.complete - msg_fields['duration'] = workflow.duration - msg_fields['exitStatus'] = workflow.exitStatus - msg_fields['errorMessage'] = (workflow.errorMessage ?: 'None') - msg_fields['errorReport'] = (workflow.errorReport ?: 'None') - msg_fields['commandLine'] = workflow.commandLine.replaceFirst(/ +--hook_url +[^ ]+/, "") - msg_fields['projectDir'] = workflow.projectDir - msg_fields['summary'] = summary << misc_fields - - // Render the JSON template - def engine = new groovy.text.GStringTemplateEngine() - // Different JSON depending on the service provider - // Defaults to "Adaptive Cards" (https://adaptivecards.io), except Slack which has its own format - def json_path = hook_url.contains("hooks.slack.com") ? "slackreport.json" : "adaptivecard.json" - def hf = new File("$projectDir/assets/${json_path}") - def json_template = engine.createTemplate(hf).make(msg_fields) - def json_message = json_template.toString() - - // POST - def post = new URL(hook_url).openConnection(); - post.setRequestMethod("POST") - post.setDoOutput(true) - post.setRequestProperty("Content-Type", "application/json") - post.getOutputStream().write(json_message.getBytes("UTF-8")); - def postRC = post.getResponseCode(); - if (! postRC.equals(200)) { - log.warn(post.getErrorStream().getText()); - } - } - - // - // Dump pipeline parameters in a json file - // - public static void dump_parameters(workflow, params) { - def timestamp = new java.util.Date().format( 'yyyy-MM-dd_HH-mm-ss') - def filename = "params_${timestamp}.json" - def temp_pf = new File(workflow.launchDir.toString(), ".${filename}") - def jsonStr = JsonOutput.toJson(params) - temp_pf.text = JsonOutput.prettyPrint(jsonStr) - - FilesEx.copyTo(temp_pf.toPath(), "${params.outdir}/pipeline_info/params_${timestamp}.json") - temp_pf.delete() - } - - // - // Print pipeline summary on completion - // - public static void summary(workflow, params, log) { - Map colors = logColours(params.monochrome_logs) - if (workflow.success) { - if (workflow.stats.ignoredCount == 0) { - log.info "-${colors.purple}[$workflow.manifest.name]${colors.green} Pipeline completed successfully${colors.reset}-" - } else { - log.info "-${colors.purple}[$workflow.manifest.name]${colors.yellow} Pipeline completed successfully, but with errored process(es) ${colors.reset}-" - } - } else { - log.info "-${colors.purple}[$workflow.manifest.name]${colors.red} Pipeline completed with errors${colors.reset}-" - } - } - - // - // ANSII Colours used for terminal logging - // - public static Map logColours(Boolean monochrome_logs) { - Map colorcodes = [:] - - // Reset / Meta - colorcodes['reset'] = monochrome_logs ? '' : "\033[0m" - colorcodes['bold'] = monochrome_logs ? '' : "\033[1m" - colorcodes['dim'] = monochrome_logs ? '' : "\033[2m" - colorcodes['underlined'] = monochrome_logs ? '' : "\033[4m" - colorcodes['blink'] = monochrome_logs ? '' : "\033[5m" - colorcodes['reverse'] = monochrome_logs ? '' : "\033[7m" - colorcodes['hidden'] = monochrome_logs ? '' : "\033[8m" - - // Regular Colors - colorcodes['black'] = monochrome_logs ? '' : "\033[0;30m" - colorcodes['red'] = monochrome_logs ? '' : "\033[0;31m" - colorcodes['green'] = monochrome_logs ? '' : "\033[0;32m" - colorcodes['yellow'] = monochrome_logs ? '' : "\033[0;33m" - colorcodes['blue'] = monochrome_logs ? '' : "\033[0;34m" - colorcodes['purple'] = monochrome_logs ? '' : "\033[0;35m" - colorcodes['cyan'] = monochrome_logs ? '' : "\033[0;36m" - colorcodes['white'] = monochrome_logs ? '' : "\033[0;37m" - - // Bold - colorcodes['bblack'] = monochrome_logs ? '' : "\033[1;30m" - colorcodes['bred'] = monochrome_logs ? '' : "\033[1;31m" - colorcodes['bgreen'] = monochrome_logs ? '' : "\033[1;32m" - colorcodes['byellow'] = monochrome_logs ? '' : "\033[1;33m" - colorcodes['bblue'] = monochrome_logs ? '' : "\033[1;34m" - colorcodes['bpurple'] = monochrome_logs ? '' : "\033[1;35m" - colorcodes['bcyan'] = monochrome_logs ? '' : "\033[1;36m" - colorcodes['bwhite'] = monochrome_logs ? '' : "\033[1;37m" - - // Underline - colorcodes['ublack'] = monochrome_logs ? '' : "\033[4;30m" - colorcodes['ured'] = monochrome_logs ? '' : "\033[4;31m" - colorcodes['ugreen'] = monochrome_logs ? '' : "\033[4;32m" - colorcodes['uyellow'] = monochrome_logs ? '' : "\033[4;33m" - colorcodes['ublue'] = monochrome_logs ? '' : "\033[4;34m" - colorcodes['upurple'] = monochrome_logs ? '' : "\033[4;35m" - colorcodes['ucyan'] = monochrome_logs ? '' : "\033[4;36m" - colorcodes['uwhite'] = monochrome_logs ? '' : "\033[4;37m" - - // High Intensity - colorcodes['iblack'] = monochrome_logs ? '' : "\033[0;90m" - colorcodes['ired'] = monochrome_logs ? '' : "\033[0;91m" - colorcodes['igreen'] = monochrome_logs ? '' : "\033[0;92m" - colorcodes['iyellow'] = monochrome_logs ? '' : "\033[0;93m" - colorcodes['iblue'] = monochrome_logs ? '' : "\033[0;94m" - colorcodes['ipurple'] = monochrome_logs ? '' : "\033[0;95m" - colorcodes['icyan'] = monochrome_logs ? '' : "\033[0;96m" - colorcodes['iwhite'] = monochrome_logs ? '' : "\033[0;97m" - - // Bold High Intensity - colorcodes['biblack'] = monochrome_logs ? '' : "\033[1;90m" - colorcodes['bired'] = monochrome_logs ? '' : "\033[1;91m" - colorcodes['bigreen'] = monochrome_logs ? '' : "\033[1;92m" - colorcodes['biyellow'] = monochrome_logs ? '' : "\033[1;93m" - colorcodes['biblue'] = monochrome_logs ? '' : "\033[1;94m" - colorcodes['bipurple'] = monochrome_logs ? '' : "\033[1;95m" - colorcodes['bicyan'] = monochrome_logs ? '' : "\033[1;96m" - colorcodes['biwhite'] = monochrome_logs ? '' : "\033[1;97m" - - return colorcodes - } - - // - // Does what is says on the tin - // - public static String dashedLine(monochrome_logs) { - Map colors = logColours(monochrome_logs) - return "-${colors.dim}----------------------------------------------------${colors.reset}-" - } - - // - // nf-core logo - // - public static String logo(workflow, monochrome_logs) { - Map colors = logColours(monochrome_logs) - String workflow_version = NfcoreTemplate.version(workflow) - String.format( - """\n - ${dashedLine(monochrome_logs)} - ${colors.purple} ${workflow.manifest.name} ${workflow_version}${colors.reset} - ${dashedLine(monochrome_logs)} - """.stripIndent() - ) - } -} diff --git a/lib/Utils.groovy b/lib/Utils.groovy deleted file mode 100644 index 8d030f4..0000000 --- a/lib/Utils.groovy +++ /dev/null @@ -1,47 +0,0 @@ -// -// This file holds several Groovy functions that could be useful for any Nextflow pipeline -// - -import org.yaml.snakeyaml.Yaml - -class Utils { - - // - // When running with -profile conda, warn if channels have not been set-up appropriately - // - public static void checkCondaChannels(log) { - Yaml parser = new Yaml() - def channels = [] - try { - def config = parser.load("conda config --show channels".execute().text) - channels = config.channels - } catch(NullPointerException | IOException e) { - log.warn "Could not verify conda channel configuration." - return - } - - // Check that all channels are present - // This channel list is ordered by required channel priority. - def required_channels_in_order = ['conda-forge', 'bioconda', 'defaults'] - def channels_missing = ((required_channels_in_order as Set) - (channels as Set)) as Boolean - - // Check that they are in the right order - def channel_priority_violation = false - def n = required_channels_in_order.size() - for (int i = 0; i < n - 1; i++) { - channel_priority_violation |= !(channels.indexOf(required_channels_in_order[i]) < channels.indexOf(required_channels_in_order[i+1])) - } - - if (channels_missing | channel_priority_violation) { - log.warn "~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~\n" + - " There is a problem with your Conda configuration!\n\n" + - " You will need to set-up the conda-forge and bioconda channels correctly.\n" + - " Please refer to https://bioconda.github.io/\n" + - " The observed channel order is \n" + - " ${channels}\n" + - " but the following channel order is required:\n" + - " ${required_channels_in_order}\n" + - "~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~" - } - } -} diff --git a/lib/WorkflowMain.groovy b/lib/WorkflowMain.groovy deleted file mode 100755 index 7a10b73..0000000 --- a/lib/WorkflowMain.groovy +++ /dev/null @@ -1,63 +0,0 @@ -// -// This file holds several functions specific to the main.nf workflow in the CenterForMedicalGeneticsGhent/nf-cmgg-exomecnv pipeline -// - -import nextflow.Nextflow - -class WorkflowMain { - - // - // Citation string for pipeline - // - public static String citation(workflow) { - return "If you use ${workflow.manifest.name} for your analysis please cite:\n\n" + - // TODO nf-core: Add Zenodo DOI for pipeline after first release - //"* The pipeline\n" + - //" https://doi.org/10.5281/zenodo.XXXXXXX\n\n" + - "* The nf-core framework\n" + - " https://doi.org/10.1038/s41587-020-0439-x\n\n" + - "* Software dependencies\n" + - " https://github.com/${workflow.manifest.name}/blob/master/CITATIONS.md" - } - - - // - // Validate parameters and print summary to screen - // - public static void initialise(workflow, params, log) { - - // Print workflow version and exit on --version - if (params.version) { - String workflow_version = NfcoreTemplate.version(workflow) - log.info "${workflow.manifest.name} ${workflow_version}" - System.exit(0) - } - - // Check that a -profile or Nextflow config has been provided to run the pipeline - NfcoreTemplate.checkConfigProvided(workflow, log) - - // Check that conda channels are set-up correctly - if (workflow.profile.tokenize(',').intersect(['conda', 'mamba']).size() >= 1) { - Utils.checkCondaChannels(log) - } - - // Check AWS batch settings - NfcoreTemplate.awsBatch(workflow, params) - - // Check input has been provided - if (!params.input) { - Nextflow.error("Please provide an input samplesheet to the pipeline e.g. '--input samplesheet.csv'") - } - } - // - // Get attribute from genome config file e.g. fasta - // - public static Object getGenomeAttribute(params, attribute) { - if (params.genomes && params.genome && params.genomes.containsKey(params.genome)) { - if (params.genomes[ params.genome ].containsKey(attribute)) { - return params.genomes[ params.genome ][ attribute ] - } - } - return null - } -} diff --git a/lib/WorkflowNf-cmgg-exomecnv.groovy b/lib/WorkflowNf-cmgg-exomecnv.groovy deleted file mode 100755 index 8e4da88..0000000 --- a/lib/WorkflowNf-cmgg-exomecnv.groovy +++ /dev/null @@ -1,122 +0,0 @@ -// -// This file holds several functions specific to the workflow/nf-cmgg-exomecnv.nf in the CenterForMedicalGeneticsGhent/nf-cmgg-exomecnv pipeline -// - -import nextflow.Nextflow -import groovy.text.SimpleTemplateEngine - -class WorkflowNf-cmgg-exomecnv { - - // - // Check and validate parameters - // - public static void initialise(params, log) { - - genomeExistsError(params, log) - - - if (!params.fasta) { - Nextflow.error "Genome fasta file not specified with e.g. '--fasta genome.fa' or via a detectable config file." - } - } - - // - // Get workflow summary for MultiQC - // - public static String paramsSummaryMultiqc(workflow, summary) { - String summary_section = '' - for (group in summary.keySet()) { - def group_params = summary.get(group) // This gets the parameters of that particular group - if (group_params) { - summary_section += "

$group

\n" - summary_section += "
\n" - for (param in group_params.keySet()) { - summary_section += "
$param
${group_params.get(param) ?: 'N/A'}
\n" - } - summary_section += "
\n" - } - } - - String yaml_file_text = "id: '${workflow.manifest.name.replace('/','-')}-summary'\n" - yaml_file_text += "description: ' - this information is collected when the pipeline is started.'\n" - yaml_file_text += "section_name: '${workflow.manifest.name} Workflow Summary'\n" - yaml_file_text += "section_href: 'https://github.com/${workflow.manifest.name}'\n" - yaml_file_text += "plot_type: 'html'\n" - yaml_file_text += "data: |\n" - yaml_file_text += "${summary_section}" - return yaml_file_text - } - - // - // Generate methods description for MultiQC - // - - public static String toolCitationText(params) { - - // TODO nf-core: Optionally add in-text citation tools to this list. - // Can use ternary operators to dynamically construct based conditions, e.g. params["run_xyz"] ? "Tool (Foo et al. 2023)" : "", - // Uncomment function in methodsDescriptionText to render in MultiQC report - def citation_text = [ - "Tools used in the workflow included:", - "FastQC (Andrews 2010),", - "MultiQC (Ewels et al. 2016)", - "." - ].join(' ').trim() - - return citation_text - } - - public static String toolBibliographyText(params) { - - // TODO Optionally add bibliographic entries to this list. - // Can use ternary operators to dynamically construct based conditions, e.g. params["run_xyz"] ? "
  • Author (2023) Pub name, Journal, DOI
    • " : "", - // Uncomment function in methodsDescriptionText to render in MultiQC report - def reference_text = [ - "
  • Andrews S, (2010) FastQC, URL: https://www.bioinformatics.babraham.ac.uk/projects/fastqc/).
    • ", - "
  • Ewels, P., Magnusson, M., Lundin, S., & Käller, M. (2016). MultiQC: summarize analysis results for multiple tools and samples in a single report. Bioinformatics , 32(19), 3047–3048. doi: /10.1093/bioinformatics/btw354
    • " - ].join(' ').trim() - - return reference_text - } - - public static String methodsDescriptionText(run_workflow, mqc_methods_yaml, params) { - // Convert to a named map so can be used as with familar NXF ${workflow} variable syntax in the MultiQC YML file - def meta = [:] - meta.workflow = run_workflow.toMap() - meta["manifest_map"] = run_workflow.manifest.toMap() - - // Pipeline DOI - meta["doi_text"] = meta.manifest_map.doi ? "(doi: ${meta.manifest_map.doi})" : "" - meta["nodoi_text"] = meta.manifest_map.doi ? "": "
  • If available, make sure to update the text to include the Zenodo DOI of version of the pipeline used.
    • " - - // Tool references - meta["tool_citations"] = "" - meta["tool_bibliography"] = "" - - // TODO Only uncomment below if logic in toolCitationText/toolBibliographyText has been filled! - //meta["tool_citations"] = toolCitationText(params).replaceAll(", \\.", ".").replaceAll("\\. \\.", ".").replaceAll(", \\.", ".") - //meta["tool_bibliography"] = toolBibliographyText(params) - - - def methods_text = mqc_methods_yaml.text - - def engine = new SimpleTemplateEngine() - def description_html = engine.createTemplate(methods_text).make(meta) - - return description_html - } - - // - // Exit pipeline if incorrect --genome key provided - // - private static void genomeExistsError(params, log) { - if (params.genomes && params.genome && !params.genomes.containsKey(params.genome)) { - def error_string = "~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~\n" + - " Genome '${params.genome}' not found in any config files provided to the pipeline.\n" + - " Currently, the available genome keys are:\n" + - " ${params.genomes.keySet().join(", ")}\n" + - "~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~" - Nextflow.error(error_string) - } - } -} diff --git a/lib/nfcore_external_java_deps.jar b/lib/nfcore_external_java_deps.jar deleted file mode 100644 index 805c8bb5e4fd43a12a5891eea5a68788309629b0..0000000000000000000000000000000000000000 GIT binary patch literal 0 HcmV?d00001 literal 2291171 zcma%i1CS<7mu}m(-Ea4_?dhK8v~AnAZQHhO+qP}n-E-&Lf8*Yb{bM(7MpZ;s){{|L znGpxiIY(9k6buar3JMCSNkdW|=)W{*ATS^)VMTsgF=-L{?{Od?P#{?eNT`1hApZ%I z{U0Zz{YCuW$x{5%Vj{u{igZ#U4^op8k`lCZbMO+hRMV3)4e|_&%)3YSbmCGpVp4NX zni_mKLGakt&P12{r^KCD0GPa9sIuy`fu+agt?8O-hY9i{WloGe}y@kSsDEY_Fud9 z-?2ab4J)MQXe4E3Xl-U<>PRMQZ)0m@@90KnV5#Te5Tz`pvZ0Lj4F<9%Flh(`Qb;Tn zMV!iFmH{S^fF*j#MCKRUd`7m>9||*;WE32I_6pg4-Q1C#ke$$aVmP-TtfJ+j+N9;Q z>bxN=bCVQkg$Jzty71(+~A9}ZfANuYIVnTdW!??Sj)Ti-Qpk8XgPDab8pk2QD&dwUl=iFhd1c$C2uMVagr zWc@z9s$#g__{3?VGb#)^fSNp^lZh 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: https://github.com/CenterForMedicalGeneticsGhent/nf-cmgg-exomecnv + Github : https://github.com/nf-cmgg/exomecnv ---------------------------------------------------------------------------------------- */ nextflow.enable.dsl = 2 +/* +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ + IMPORT FUNCTIONS / MODULES / SUBWORKFLOWS / WORKFLOWS +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ +*/ + +include { EXOMECNV } from './workflows/exomecnv' +include { PIPELINE_INITIALISATION } from './subworkflows/local/utils_nfcore_exomecnv_pipeline' +include { PIPELINE_COMPLETION } from './subworkflows/local/utils_nfcore_exomecnv_pipeline' + +include { getGenomeAttribute } from './subworkflows/local/utils_nfcore_exomecnv_pipeline' + /* ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ GENOME PARAMETER VALUES @@ -18,59 +30,77 @@ nextflow.enable.dsl = 2 // TODO nf-core: Remove this line if you don't need a FASTA file // This is an example of how to use getGenomeAttribute() to fetch parameters // from igenomes.config using `--genome` -params.fasta = WorkflowMain.getGenomeAttribute(params, 'fasta') +params.fasta = getGenomeAttribute('fasta') /* ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ - VALIDATE & PRINT PARAMETER SUMMARY + NAMED WORKFLOWS FOR PIPELINE ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ */ -include { validateParameters; paramsHelp } from 'plugin/nf-validation' +// +// WORKFLOW: Run main analysis pipeline depending on type of input +// +workflow NFCMGG_EXOMECNV { -// Print help message if needed -if (params.help) { - def logo = NfcoreTemplate.logo(workflow, params.monochrome_logs) - def citation = '\n' + WorkflowMain.citation(workflow) + '\n' - def String command = "nextflow run ${workflow.manifest.name} --input samplesheet.csv --genome GRCh37 -profile docker" - log.info logo + paramsHelp(command) + citation + NfcoreTemplate.dashedLine(params.monochrome_logs) - System.exit(0) -} + take: + samplesheet // channel: samplesheet read in from --input -// Validate input parameters -if (params.validate_params) { - validateParameters() -} + main: -WorkflowMain.initialise(workflow, params, log) + // + // WORKFLOW: Run pipeline + // + EXOMECNV ( + samplesheet + ) -/* -~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ - NAMED WORKFLOW FOR PIPELINE -~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ -*/ - -include { NF-CMGG-EXOMECNV } from './workflows/nf-cmgg-exomecnv' + emit: + multiqc_report = EXOMECNV.out.multiqc_report // channel: /path/to/multiqc_report.html -// -// WORKFLOW: Run main CenterForMedicalGeneticsGhent/nf-cmgg-exomecnv analysis pipeline -// -workflow CENTERFORMEDICALGENETICSGHENT_NF-CMGG-EXOMECNV { - NF-CMGG-EXOMECNV () } - /* ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ - RUN ALL WORKFLOWS + RUN MAIN WORKFLOW ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ */ -// -// WORKFLOW: Execute a single named workflow for the pipeline -// See: https://github.com/nf-core/rnaseq/issues/619 -// workflow { - CENTERFORMEDICALGENETICSGHENT_NF-CMGG-EXOMECNV () + + main: + + // + // SUBWORKFLOW: Run initialisation tasks + // + PIPELINE_INITIALISATION ( + params.version, + params.help, + params.validate_params, + params.monochrome_logs, + args, + params.outdir, + params.input + ) + + // + // WORKFLOW: Run main workflow + // + NFCMGG_EXOMECNV ( + PIPELINE_INITIALISATION.out.samplesheet + ) + + // + // SUBWORKFLOW: Run completion tasks + // + PIPELINE_COMPLETION ( + params.email, + params.email_on_fail, + params.plaintext_email, + params.outdir, + params.monochrome_logs, + params.hook_url, + NFCMGG_EXOMECNV.out.multiqc_report + ) } /* diff --git a/modules.json b/modules.json index ce86deb..01ff1f0 100644 --- a/modules.json +++ b/modules.json @@ -1,26 +1,40 @@ { - "name": "CenterForMedicalGeneticsGhent/nf-cmgg-exomecnv", - "homePage": "https://github.com/CenterForMedicalGeneticsGhent/nf-cmgg-exomecnv", + "name": "nf-cmgg/exomecnv", + "homePage": "https://github.com/nf-cmgg/exomecnv", "repos": { "https://github.com/nf-core/modules.git": { "modules": { "nf-core": { - "custom/dumpsoftwareversions": { - "branch": "master", - "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", - "installed_by": ["modules"] - }, "fastqc": { "branch": "master", - "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", + "git_sha": "f4ae1d942bd50c5c0b9bd2de1393ce38315ba57c", "installed_by": ["modules"] }, "multiqc": { "branch": "master", - "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", + "git_sha": "b7ebe95761cd389603f9cc0e0dc384c0f663815a", "installed_by": ["modules"] } } + }, + "subworkflows": { + "nf-core": { + "utils_nextflow_pipeline": { + "branch": "master", + "git_sha": "5caf7640a9ef1d18d765d55339be751bb0969dfa", + "installed_by": ["subworkflows"] + }, + "utils_nfcore_pipeline": { + "branch": "master", + "git_sha": "5caf7640a9ef1d18d765d55339be751bb0969dfa", + "installed_by": ["subworkflows"] + }, + "utils_nfvalidation_plugin": { + "branch": "master", + "git_sha": "5caf7640a9ef1d18d765d55339be751bb0969dfa", + "installed_by": ["subworkflows"] + } + } } } } diff --git a/modules/local/samplesheet_check.nf b/modules/local/samplesheet_check.nf deleted file mode 100644 index 77efa85..0000000 --- a/modules/local/samplesheet_check.nf +++ /dev/null @@ -1,31 +0,0 @@ -process SAMPLESHEET_CHECK { - tag "$samplesheet" - label 'process_single' - - conda "conda-forge::python=3.8.3" - container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/python:3.8.3' : - 'biocontainers/python:3.8.3' }" - - input: - path samplesheet - - output: - path '*.csv' , emit: csv - path "versions.yml", emit: versions - - when: - task.ext.when == null || task.ext.when - - script: // This script is bundled with the pipeline, in CenterForMedicalGeneticsGhent/nf-cmgg-exomecnv/bin/ - """ - check_samplesheet.py \\ - $samplesheet \\ - samplesheet.valid.csv - - cat <<-END_VERSIONS > versions.yml - "${task.process}": - python: \$(python --version | sed 's/Python //g') - END_VERSIONS - """ -} diff --git a/modules/nf-core/custom/dumpsoftwareversions/environment.yml b/modules/nf-core/custom/dumpsoftwareversions/environment.yml deleted file mode 100644 index f0c63f6..0000000 --- a/modules/nf-core/custom/dumpsoftwareversions/environment.yml +++ /dev/null @@ -1,7 +0,0 @@ -name: custom_dumpsoftwareversions -channels: - - conda-forge - - bioconda - - defaults -dependencies: - - bioconda::multiqc=1.17 diff --git a/modules/nf-core/custom/dumpsoftwareversions/main.nf b/modules/nf-core/custom/dumpsoftwareversions/main.nf deleted file mode 100644 index 7685b33..0000000 --- a/modules/nf-core/custom/dumpsoftwareversions/main.nf +++ /dev/null @@ -1,24 +0,0 @@ -process CUSTOM_DUMPSOFTWAREVERSIONS { - label 'process_single' - - // Requires `pyyaml` which does not have a dedicated container but is in the MultiQC container - conda "${moduleDir}/environment.yml" - container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/multiqc:1.17--pyhdfd78af_0' : - 'biocontainers/multiqc:1.17--pyhdfd78af_0' }" - - input: - path versions - - output: - path "software_versions.yml" , emit: yml - path "software_versions_mqc.yml", emit: mqc_yml - path "versions.yml" , emit: versions - - when: - task.ext.when == null || task.ext.when - - script: - def args = task.ext.args ?: '' - template 'dumpsoftwareversions.py' -} diff --git a/modules/nf-core/custom/dumpsoftwareversions/meta.yml b/modules/nf-core/custom/dumpsoftwareversions/meta.yml deleted file mode 100644 index 9414c32..0000000 --- a/modules/nf-core/custom/dumpsoftwareversions/meta.yml +++ /dev/null @@ -1,37 +0,0 @@ -# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/yaml-schema.json -name: custom_dumpsoftwareversions -description: Custom module used to dump software versions within the nf-core pipeline template -keywords: - - custom - - dump - - version -tools: - - custom: - description: Custom module used to dump software versions within the nf-core pipeline template - homepage: https://github.com/nf-core/tools - documentation: https://github.com/nf-core/tools - licence: ["MIT"] -input: - - versions: - type: file - description: YML file containing software versions - pattern: "*.yml" -output: - - yml: - type: file - description: Standard YML file containing software versions - pattern: "software_versions.yml" - - mqc_yml: - type: file - description: MultiQC custom content YML file containing software versions - pattern: "software_versions_mqc.yml" - - versions: - type: file - description: File containing software versions - pattern: "versions.yml" -authors: - - "@drpatelh" - - "@grst" -maintainers: - - "@drpatelh" - - "@grst" diff --git a/modules/nf-core/custom/dumpsoftwareversions/templates/dumpsoftwareversions.py b/modules/nf-core/custom/dumpsoftwareversions/templates/dumpsoftwareversions.py deleted file mode 100755 index e55b8d4..0000000 --- a/modules/nf-core/custom/dumpsoftwareversions/templates/dumpsoftwareversions.py +++ /dev/null @@ -1,102 +0,0 @@ -#!/usr/bin/env python - - -"""Provide functions to merge multiple versions.yml files.""" - - -import platform -from textwrap import dedent - -import yaml - - -def _make_versions_html(versions): - """Generate a tabular HTML output of all versions for MultiQC.""" - html = [ - dedent( - """\\ - - - - - - - - - - """ - ) - ] - for process, tmp_versions in sorted(versions.items()): - html.append("") - for i, (tool, version) in enumerate(sorted(tmp_versions.items())): - html.append( - dedent( - f"""\\ - - - - - - """ - ) - ) - html.append("") - html.append("
    Process Name Software Version
    {process if (i == 0) else ''}{tool}{version}
    ") - return "\\n".join(html) - - -def main(): - """Load all version files and generate merged output.""" - versions_this_module = {} - versions_this_module["${task.process}"] = { - "python": platform.python_version(), - "yaml": yaml.__version__, - } - - with open("$versions") as f: - versions_by_process = yaml.load(f, Loader=yaml.BaseLoader) | versions_this_module - - # aggregate versions by the module name (derived from fully-qualified process name) - versions_by_module = {} - for process, process_versions in versions_by_process.items(): - module = process.split(":")[-1] - try: - if versions_by_module[module] != process_versions: - raise AssertionError( - "We assume that software versions are the same between all modules. " - "If you see this error-message it means you discovered an edge-case " - "and should open an issue in nf-core/tools. " - ) - except KeyError: - versions_by_module[module] = process_versions - - versions_by_module["Workflow"] = { - "Nextflow": "$workflow.nextflow.version", - "$workflow.manifest.name": "$workflow.manifest.version", - } - - versions_mqc = { - "id": "software_versions", - "section_name": "${workflow.manifest.name} Software Versions", - "section_href": "https://github.com/${workflow.manifest.name}", - "plot_type": "html", - "description": "are collected at run time from the software output.", - "data": _make_versions_html(versions_by_module), - } - - with open("software_versions.yml", "w") as f: - yaml.dump(versions_by_module, f, default_flow_style=False) - with open("software_versions_mqc.yml", "w") as f: - yaml.dump(versions_mqc, f, default_flow_style=False) - - with open("versions.yml", "w") as f: - yaml.dump(versions_this_module, f, default_flow_style=False) - - -if __name__ == "__main__": - main() diff --git a/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test b/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test deleted file mode 100644 index eec1db1..0000000 --- a/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test +++ /dev/null @@ -1,38 +0,0 @@ -nextflow_process { - - name "Test Process CUSTOM_DUMPSOFTWAREVERSIONS" - script "../main.nf" - process "CUSTOM_DUMPSOFTWAREVERSIONS" - tag "modules" - tag "modules_nfcore" - tag "custom" - tag "dumpsoftwareversions" - tag "custom/dumpsoftwareversions" - - test("Should run without failures") { - when { - process { - """ - def tool1_version = ''' - TOOL1: - tool1: 0.11.9 - '''.stripIndent() - - def tool2_version = ''' - TOOL2: - tool2: 1.9 - '''.stripIndent() - - input[0] = Channel.of(tool1_version, tool2_version).collectFile() - """ - } - } - - then { - assertAll( - { assert process.success }, - { assert snapshot(process.out).match() } - ) - } - } -} diff --git a/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test.snap b/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test.snap deleted file mode 100644 index 4274ed5..0000000 --- a/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test.snap +++ /dev/null @@ -1,27 +0,0 @@ -{ - "Should run without failures": { - "content": [ - { - "0": [ - "software_versions.yml:md5,1c851188476409cda5752ce971b20b58" - ], - "1": [ - "software_versions_mqc.yml:md5,2570f4ba271ad08357b0d3d32a9cf84d" - ], - "2": [ - "versions.yml:md5,3843ac526e762117eedf8825b40683df" - ], - "mqc_yml": [ - "software_versions_mqc.yml:md5,2570f4ba271ad08357b0d3d32a9cf84d" - ], - "versions": [ - "versions.yml:md5,3843ac526e762117eedf8825b40683df" - ], - "yml": [ - "software_versions.yml:md5,1c851188476409cda5752ce971b20b58" - ] - } - ], - "timestamp": "2023-11-03T14:43:22.157011" - } -} diff --git a/modules/nf-core/custom/dumpsoftwareversions/tests/tags.yml b/modules/nf-core/custom/dumpsoftwareversions/tests/tags.yml deleted file mode 100644 index 405aa24..0000000 --- a/modules/nf-core/custom/dumpsoftwareversions/tests/tags.yml +++ /dev/null @@ -1,2 +0,0 @@ -custom/dumpsoftwareversions: - - modules/nf-core/custom/dumpsoftwareversions/** diff --git a/modules/nf-core/fastqc/main.nf b/modules/nf-core/fastqc/main.nf index 50e59f2..9e19a74 100644 --- a/modules/nf-core/fastqc/main.nf +++ b/modules/nf-core/fastqc/main.nf @@ -37,7 +37,7 @@ process FASTQC { cat <<-END_VERSIONS > versions.yml "${task.process}": - fastqc: \$( fastqc --version | sed -e "s/FastQC v//g" ) + fastqc: \$( fastqc --version | sed '/FastQC v/!d; s/.*v//' ) END_VERSIONS """ @@ -49,7 +49,7 @@ process FASTQC { cat <<-END_VERSIONS > versions.yml "${task.process}": - fastqc: \$( fastqc --version | sed -e "s/FastQC v//g" ) + fastqc: \$( fastqc --version | sed '/FastQC v/!d; s/.*v//' ) END_VERSIONS """ } diff --git a/modules/nf-core/fastqc/tests/main.nf.test b/modules/nf-core/fastqc/tests/main.nf.test index 6437a14..70edae4 100644 --- a/modules/nf-core/fastqc/tests/main.nf.test +++ b/modules/nf-core/fastqc/tests/main.nf.test @@ -3,24 +3,20 @@ nextflow_process { name "Test Process FASTQC" script "../main.nf" process "FASTQC" + tag "modules" tag "modules_nfcore" tag "fastqc" - test("Single-Read") { + test("sarscov2 single-end [fastq]") { when { - params { - outdir = "$outputDir" - } process { """ - input[0] = [ + input[0] = Channel.of([ [ id: 'test', single_end:true ], - [ - file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true) - ] - ] + [ file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true) ] + ]) """ } } @@ -28,14 +24,189 @@ nextflow_process { then { assertAll ( { assert process.success }, + // NOTE The report contains the date inside it, which means that the md5sum is stable per day, but not longer than that. So you can't md5sum it. // looks like this:

    // https://github.com/nf-core/modules/pull/3903#issuecomment-1743620039 - { assert process.out.html.get(0).get(1) ==~ ".*/test_fastqc.html" }, - { assert path(process.out.html.get(0).get(1)).getText().contains("File typeConventional base calls") }, - { assert snapshot(process.out.versions).match("versions") }, - { assert process.out.zip.get(0).get(1) ==~ ".*/test_fastqc.zip" } + + { assert process.out.html[0][1] ==~ ".*/test_fastqc.html" }, + { assert process.out.zip[0][1] ==~ ".*/test_fastqc.zip" }, + { assert path(process.out.html[0][1]).text.contains("File typeConventional base calls") }, + + { assert snapshot(process.out.versions).match("fastqc_versions_single") } + ) + } + } + + test("sarscov2 paired-end [fastq]") { + + when { + process { + """ + input[0] = Channel.of([ + [id: 'test', single_end: false], // meta map + [ file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true), + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_2.fastq.gz', checkIfExists: true) ] + ]) + """ + } + } + + then { + assertAll ( + { assert process.success }, + + { assert process.out.html[0][1][0] ==~ ".*/test_1_fastqc.html" }, + { assert process.out.html[0][1][1] ==~ ".*/test_2_fastqc.html" }, + { assert process.out.zip[0][1][0] ==~ ".*/test_1_fastqc.zip" }, + { assert process.out.zip[0][1][1] ==~ ".*/test_2_fastqc.zip" }, + { assert path(process.out.html[0][1][0]).text.contains("File typeConventional base calls") }, + { assert path(process.out.html[0][1][1]).text.contains("File typeConventional base calls") }, + + { assert snapshot(process.out.versions).match("fastqc_versions_paired") } + ) + } + } + + test("sarscov2 interleaved [fastq]") { + + when { + process { + """ + input[0] = Channel.of([ + [id: 'test', single_end: false], // meta map + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_interleaved.fastq.gz', checkIfExists: true) + ]) + """ + } + } + + then { + assertAll ( + { assert process.success }, + + { assert process.out.html[0][1] ==~ ".*/test_fastqc.html" }, + { assert process.out.zip[0][1] ==~ ".*/test_fastqc.zip" }, + { assert path(process.out.html[0][1]).text.contains("File typeConventional base calls") }, + + { assert snapshot(process.out.versions).match("fastqc_versions_interleaved") } ) } } + + test("sarscov2 paired-end [bam]") { + + when { + process { + """ + input[0] = Channel.of([ + [id: 'test', single_end: false], // meta map + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/bam/test.paired_end.sorted.bam', checkIfExists: true) + ]) + """ + } + } + + then { + assertAll ( + { assert process.success }, + + { assert process.out.html[0][1] ==~ ".*/test_fastqc.html" }, + { assert process.out.zip[0][1] ==~ ".*/test_fastqc.zip" }, + { assert path(process.out.html[0][1]).text.contains("File typeConventional base calls") }, + + { assert snapshot(process.out.versions).match("fastqc_versions_bam") } + ) + } + } + + test("sarscov2 multiple [fastq]") { + + when { + process { + """ + input[0] = Channel.of([ + [id: 'test', single_end: false], // meta map + [ file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true), + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_2.fastq.gz', checkIfExists: true), + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test2_1.fastq.gz', checkIfExists: true), + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test2_2.fastq.gz', checkIfExists: true) ] + ]) + """ + } + } + + then { + assertAll ( + { assert process.success }, + + { assert process.out.html[0][1][0] ==~ ".*/test_1_fastqc.html" }, + { assert process.out.html[0][1][1] ==~ ".*/test_2_fastqc.html" }, + { assert process.out.html[0][1][2] ==~ ".*/test_3_fastqc.html" }, + { assert process.out.html[0][1][3] ==~ ".*/test_4_fastqc.html" }, + { assert process.out.zip[0][1][0] ==~ ".*/test_1_fastqc.zip" }, + { assert process.out.zip[0][1][1] ==~ ".*/test_2_fastqc.zip" }, + { assert process.out.zip[0][1][2] ==~ ".*/test_3_fastqc.zip" }, + { assert process.out.zip[0][1][3] ==~ ".*/test_4_fastqc.zip" }, + { assert path(process.out.html[0][1][0]).text.contains("File typeConventional base calls") }, + { assert path(process.out.html[0][1][1]).text.contains("File typeConventional base calls") }, + { assert path(process.out.html[0][1][2]).text.contains("File typeConventional base calls") }, + { assert path(process.out.html[0][1][3]).text.contains("File typeConventional base calls") }, + + { assert snapshot(process.out.versions).match("fastqc_versions_multiple") } + ) + } + } + + test("sarscov2 custom_prefix") { + + when { + process { + """ + input[0] = Channel.of([ + [ id:'mysample', single_end:true ], // meta map + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true) + ]) + """ + } + } + + then { + assertAll ( + { assert process.success }, + + { assert process.out.html[0][1] ==~ ".*/mysample_fastqc.html" }, + { assert process.out.zip[0][1] ==~ ".*/mysample_fastqc.zip" }, + { assert path(process.out.html[0][1]).text.contains("File typeConventional base calls") }, + + { assert snapshot(process.out.versions).match("fastqc_versions_custom_prefix") } + ) + } + } + + test("sarscov2 single-end [fastq] - stub") { + + options "-stub" + + when { + process { + """ + input[0] = Channel.of([ + [ id: 'test', single_end:true ], + [ file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true) ] + ]) + """ + } + } + + then { + assertAll ( + { assert process.success }, + { assert snapshot(process.out.html.collect { file(it[1]).getName() } + + process.out.zip.collect { file(it[1]).getName() } + + process.out.versions ).match("fastqc_stub") } + ) + } + } + } diff --git a/modules/nf-core/fastqc/tests/main.nf.test.snap b/modules/nf-core/fastqc/tests/main.nf.test.snap index 636a32c..86f7c31 100644 --- a/modules/nf-core/fastqc/tests/main.nf.test.snap +++ b/modules/nf-core/fastqc/tests/main.nf.test.snap @@ -1,10 +1,88 @@ { - "versions": { + "fastqc_versions_interleaved": { "content": [ [ "versions.yml:md5,e1cc25ca8af856014824abd842e93978" ] ], - "timestamp": "2023-10-09T23:40:54+0000" + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-01-31T17:40:07.293713" + }, + "fastqc_stub": { + "content": [ + [ + "test.html", + "test.zip", + "versions.yml:md5,e1cc25ca8af856014824abd842e93978" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-01-31T17:31:01.425198" + }, + "fastqc_versions_multiple": { + "content": [ + [ + "versions.yml:md5,e1cc25ca8af856014824abd842e93978" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-01-31T17:40:55.797907" + }, + "fastqc_versions_bam": { + "content": [ + [ + "versions.yml:md5,e1cc25ca8af856014824abd842e93978" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-01-31T17:40:26.795862" + }, + "fastqc_versions_single": { + "content": [ + [ + "versions.yml:md5,e1cc25ca8af856014824abd842e93978" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-01-31T17:39:27.043675" + }, + "fastqc_versions_paired": { + "content": [ + [ + "versions.yml:md5,e1cc25ca8af856014824abd842e93978" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-01-31T17:39:47.584191" + }, + "fastqc_versions_custom_prefix": { + "content": [ + [ + "versions.yml:md5,e1cc25ca8af856014824abd842e93978" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-01-31T17:41:14.576531" } } \ No newline at end of file diff --git a/modules/nf-core/multiqc/environment.yml b/modules/nf-core/multiqc/environment.yml index e5e14e8..ca39fb6 100644 --- a/modules/nf-core/multiqc/environment.yml +++ b/modules/nf-core/multiqc/environment.yml @@ -1,7 +1,7 @@ -name: MultiQC +name: multiqc channels: - conda-forge - bioconda - defaults dependencies: - - bioconda::multiqc=1.17 + - bioconda::multiqc=1.21 diff --git a/modules/nf-core/multiqc/main.nf b/modules/nf-core/multiqc/main.nf index 2bbc398..47ac352 100644 --- a/modules/nf-core/multiqc/main.nf +++ b/modules/nf-core/multiqc/main.nf @@ -3,8 +3,8 @@ process MULTIQC { conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/multiqc:1.17--pyhdfd78af_0' : - 'biocontainers/multiqc:1.17--pyhdfd78af_0' }" + 'https://depot.galaxyproject.org/singularity/multiqc:1.21--pyhdfd78af_0' : + 'biocontainers/multiqc:1.21--pyhdfd78af_0' }" input: path multiqc_files, stageAs: "?/*" @@ -25,12 +25,14 @@ process MULTIQC { def args = task.ext.args ?: '' def config = multiqc_config ? "--config $multiqc_config" : '' def extra_config = extra_multiqc_config ? "--config $extra_multiqc_config" : '' + def logo = multiqc_logo ? /--cl-config 'custom_logo: "${multiqc_logo}"'/ : '' """ multiqc \\ --force \\ $args \\ $config \\ $extra_config \\ + $logo \\ . cat <<-END_VERSIONS > versions.yml @@ -41,7 +43,7 @@ process MULTIQC { stub: """ - touch multiqc_data + mkdir multiqc_data touch multiqc_plots touch multiqc_report.html diff --git a/modules/nf-core/multiqc/meta.yml b/modules/nf-core/multiqc/meta.yml index a61223e..45a9bc3 100644 --- a/modules/nf-core/multiqc/meta.yml +++ b/modules/nf-core/multiqc/meta.yml @@ -1,5 +1,4 @@ -# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/yaml-schema.json -name: MultiQC +name: multiqc description: Aggregate results from bioinformatics analyses across many samples into a single report keywords: - QC diff --git a/modules/nf-core/multiqc/tests/main.nf.test b/modules/nf-core/multiqc/tests/main.nf.test new file mode 100644 index 0000000..f1c4242 --- /dev/null +++ b/modules/nf-core/multiqc/tests/main.nf.test @@ -0,0 +1,84 @@ +nextflow_process { + + name "Test Process MULTIQC" + script "../main.nf" + process "MULTIQC" + + tag "modules" + tag "modules_nfcore" + tag "multiqc" + + test("sarscov2 single-end [fastqc]") { + + when { + process { + """ + input[0] = Channel.of(file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastqc/test_fastqc.zip', checkIfExists: true)) + input[1] = [] + input[2] = [] + input[3] = [] + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert process.out.report[0] ==~ ".*/multiqc_report.html" }, + { assert process.out.data[0] ==~ ".*/multiqc_data" }, + { assert snapshot(process.out.versions).match("multiqc_versions_single") } + ) + } + + } + + test("sarscov2 single-end [fastqc] [config]") { + + when { + process { + """ + input[0] = Channel.of(file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastqc/test_fastqc.zip', checkIfExists: true)) + input[1] = Channel.of(file("https://github.com/nf-core/tools/raw/dev/nf_core/pipeline-template/assets/multiqc_config.yml", checkIfExists: true)) + input[2] = [] + input[3] = [] + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert process.out.report[0] ==~ ".*/multiqc_report.html" }, + { assert process.out.data[0] ==~ ".*/multiqc_data" }, + { assert snapshot(process.out.versions).match("multiqc_versions_config") } + ) + } + } + + test("sarscov2 single-end [fastqc] - stub") { + + options "-stub" + + when { + process { + """ + input[0] = Channel.of(file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastqc/test_fastqc.zip', checkIfExists: true)) + input[1] = [] + input[2] = [] + input[3] = [] + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(process.out.report.collect { file(it).getName() } + + process.out.data.collect { file(it).getName() } + + process.out.plots.collect { file(it).getName() } + + process.out.versions ).match("multiqc_stub") } + ) + } + + } +} diff --git a/modules/nf-core/multiqc/tests/main.nf.test.snap b/modules/nf-core/multiqc/tests/main.nf.test.snap new file mode 100644 index 0000000..bfebd80 --- /dev/null +++ b/modules/nf-core/multiqc/tests/main.nf.test.snap @@ -0,0 +1,41 @@ +{ + "multiqc_versions_single": { + "content": [ + [ + "versions.yml:md5,21f35ee29416b9b3073c28733efe4b7d" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-02-29T08:48:55.657331" + }, + "multiqc_stub": { + "content": [ + [ + "multiqc_report.html", + "multiqc_data", + "multiqc_plots", + "versions.yml:md5,21f35ee29416b9b3073c28733efe4b7d" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-02-29T08:49:49.071937" + }, + "multiqc_versions_config": { + "content": [ + [ + "versions.yml:md5,21f35ee29416b9b3073c28733efe4b7d" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-02-29T08:49:25.457567" + } +} \ No newline at end of file diff --git a/modules/nf-core/multiqc/tests/tags.yml b/modules/nf-core/multiqc/tests/tags.yml new file mode 100644 index 0000000..bea6c0d --- /dev/null +++ b/modules/nf-core/multiqc/tests/tags.yml @@ -0,0 +1,2 @@ +multiqc: + - modules/nf-core/multiqc/** diff --git a/nextflow.config b/nextflow.config index a1e9404..9be95d5 100644 --- a/nextflow.config +++ b/nextflow.config @@ -1,6 +1,6 @@ /* ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ - CenterForMedicalGeneticsGhent/nf-cmgg-exomecnv Nextflow config file + nf-cmgg/exomecnv Nextflow config file ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ Default config options for all compute environments ---------------------------------------------------------------------------------------- @@ -16,9 +16,7 @@ params { genome = null igenomes_base = 's3://ngi-igenomes/igenomes/' igenomes_ignore = false - - - // MultiQC options + fasta = null// MultiQC options multiqc_config = null multiqc_title = null multiqc_logo = null @@ -43,7 +41,6 @@ params { custom_config_base = "https://raw.githubusercontent.com/nf-core/configs/${params.custom_config_version}" config_profile_contact = null config_profile_url = null - // Max resource options // Defaults only, expecting to be overwritten @@ -70,12 +67,12 @@ try { System.err.println("WARNING: Could not load nf-core/config profiles: ${params.custom_config_base}/nfcore_custom.config") } -// Load CenterForMedicalGeneticsGhent/nf-cmgg-exomecnv custom profiles from different institutions. -// Warning: Uncomment only if a pipeline-specific instititutional config already exists on nf-core/configs! +// Load nf-cmgg/exomecnv custom profiles from different institutions. +// Warning: Uncomment only if a pipeline-specific institutional config already exists on nf-core/configs! // try { -// includeConfig "${params.custom_config_base}/pipeline/nf-cmgg-exomecnv.config" +// includeConfig "${params.custom_config_base}/pipeline/exomecnv.config" // } catch (Exception e) { -// System.err.println("WARNING: Could not load nf-core/config/nf-cmgg-exomecnv profiles: ${params.custom_config_base}/pipeline/nf-cmgg-exomecnv.config") +// System.err.println("WARNING: Could not load nf-core/config/exomecnv profiles: ${params.custom_config_base}/pipeline/exomecnv.config") // } profiles { debug { @@ -91,6 +88,7 @@ profiles { podman.enabled = false shifter.enabled = false charliecloud.enabled = false + channels = ['conda-forge', 'bioconda', 'defaults'] apptainer.enabled = false } mamba { @@ -105,17 +103,16 @@ profiles { } docker { docker.enabled = true - docker.userEmulation = true conda.enabled = false singularity.enabled = false podman.enabled = false shifter.enabled = false charliecloud.enabled = false apptainer.enabled = false - runOptions = '-u $(id -u):$(id -g)' + docker.runOptions = '-u $(id -u):$(id -g)' } arm { - docker.runOptions = '-u $(id -u):$(id -g) --platform=linux/amd64' + docker.runOptions = '-u $(id -u):$(id -g) --platform=linux/amd64' } singularity { singularity.enabled = true @@ -183,7 +180,7 @@ singularity.registry = 'quay.io' // Nextflow plugins plugins { - id 'nf-validation' // Validation of pipeline parameters and creation of an input channel from a sample sheet + id 'nf-validation@1.1.3' // Validation of pipeline parameters and creation of an input channel from a sample sheet } // Load igenomes.config if required @@ -228,9 +225,9 @@ dag { } manifest { - name = 'CenterForMedicalGeneticsGhent/nf-cmgg-exomecnv' + name = 'nf-cmgg/exomecnv' author = """nvnieuwk""" - homePage = 'https://github.com/CenterForMedicalGeneticsGhent/nf-cmgg-exomecnv' + homePage = 'https://github.com/nf-cmgg/exomecnv' description = """A nextflow pipeline for calling exome CNVs""" mainScript = 'main.nf' nextflowVersion = '!>=23.04.0' diff --git a/nextflow_schema.json b/nextflow_schema.json index 860d68e..2fe8784 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -1,7 +1,7 @@ { "$schema": "http://json-schema.org/draft-07/schema", - "$id": "https://raw.githubusercontent.com/CenterForMedicalGeneticsGhent/nf-cmgg-exomecnv/master/nextflow_schema.json", - "title": "CenterForMedicalGeneticsGhent/nf-cmgg-exomecnv pipeline parameters", + "$id": "https://raw.githubusercontent.com/nf-cmgg/exomecnv/master/nextflow_schema.json", + "title": "nf-cmgg/exomecnv pipeline parameters", "description": "A nextflow pipeline for calling exome CNVs", "type": "object", "definitions": { @@ -16,6 +16,7 @@ "type": "string", "format": "file-path", "exists": true, + "schema": "assets/schema_input.json", "mimetype": "text/csv", "pattern": "^\\S+\\.csv$", "description": "Path to comma-separated file containing information about the samples in the experiment.", diff --git a/pyproject.toml b/pyproject.toml index 0d62beb..5611062 100644 --- a/pyproject.toml +++ b/pyproject.toml @@ -1,10 +1,15 @@ -# Config file for Python. Mostly used to configure linting of bin/check_samplesheet.py with Black. +# Config file for Python. Mostly used to configure linting of bin/*.py with Ruff. # Should be kept the same as nf-core/tools to avoid fighting with template synchronisation. -[tool.black] +[tool.ruff] line-length = 120 -target_version = ["py37", "py38", "py39", "py310"] +target-version = "py38" +cache-dir = "~/.cache/ruff" -[tool.isort] -profile = "black" -known_first_party = ["nf_core"] -multi_line_output = 3 +[tool.ruff.lint] +select = ["I", "E1", "E4", "E7", "E9", "F", "UP", "N"] + +[tool.ruff.lint.isort] +known-first-party = ["nf_core"] + +[tool.ruff.lint.per-file-ignores] +"__init__.py" = ["E402", "F401"] diff --git a/subworkflows/local/input_check.nf b/subworkflows/local/input_check.nf deleted file mode 100644 index 0aecf87..0000000 --- a/subworkflows/local/input_check.nf +++ /dev/null @@ -1,44 +0,0 @@ -// -// Check input samplesheet and get read channels -// - -include { SAMPLESHEET_CHECK } from '../../modules/local/samplesheet_check' - -workflow INPUT_CHECK { - take: - samplesheet // file: /path/to/samplesheet.csv - - main: - SAMPLESHEET_CHECK ( samplesheet ) - .csv - .splitCsv ( header:true, sep:',' ) - .map { create_fastq_channel(it) } - .set { reads } - - emit: - reads // channel: [ val(meta), [ reads ] ] - versions = SAMPLESHEET_CHECK.out.versions // channel: [ versions.yml ] -} - -// Function to get list of [ meta, [ fastq_1, fastq_2 ] ] -def create_fastq_channel(LinkedHashMap row) { - // create meta map - def meta = [:] - meta.id = row.sample - meta.single_end = row.single_end.toBoolean() - - // add path(s) of the fastq file(s) to the meta map - def fastq_meta = [] - if (!file(row.fastq_1).exists()) { - exit 1, "ERROR: Please check input samplesheet -> Read 1 FastQ file does not exist!\n${row.fastq_1}" - } - if (meta.single_end) { - fastq_meta = [ meta, [ file(row.fastq_1) ] ] - } else { - if (!file(row.fastq_2).exists()) { - exit 1, "ERROR: Please check input samplesheet -> Read 2 FastQ file does not exist!\n${row.fastq_2}" - } - fastq_meta = [ meta, [ file(row.fastq_1), file(row.fastq_2) ] ] - } - return fastq_meta -} diff --git a/subworkflows/local/utils_nfcore_exomecnv_pipeline/main.nf b/subworkflows/local/utils_nfcore_exomecnv_pipeline/main.nf new file mode 100644 index 0000000..442a301 --- /dev/null +++ b/subworkflows/local/utils_nfcore_exomecnv_pipeline/main.nf @@ -0,0 +1,251 @@ +// +// Subworkflow with functionality specific to the nf-cmgg/exomecnv pipeline +// + +/* +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ + IMPORT FUNCTIONS / MODULES / SUBWORKFLOWS +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ +*/ + +include { UTILS_NFVALIDATION_PLUGIN } from '../../nf-core/utils_nfvalidation_plugin' +include { paramsSummaryMap } from 'plugin/nf-validation' +include { fromSamplesheet } from 'plugin/nf-validation' +include { UTILS_NEXTFLOW_PIPELINE } from '../../nf-core/utils_nextflow_pipeline' +include { completionEmail } from '../../nf-core/utils_nfcore_pipeline' +include { completionSummary } from '../../nf-core/utils_nfcore_pipeline' +include { dashedLine } from '../../nf-core/utils_nfcore_pipeline' +include { nfCoreLogo } from '../../nf-core/utils_nfcore_pipeline' +include { imNotification } from '../../nf-core/utils_nfcore_pipeline' +include { UTILS_NFCORE_PIPELINE } from '../../nf-core/utils_nfcore_pipeline' +include { workflowCitation } from '../../nf-core/utils_nfcore_pipeline' + +/* +======================================================================================== + SUBWORKFLOW TO INITIALISE PIPELINE +======================================================================================== +*/ + +workflow PIPELINE_INITIALISATION { + + take: + version // boolean: Display version and exit + help // boolean: Display help text + validate_params // boolean: Boolean whether to validate parameters against the schema at runtime + monochrome_logs // boolean: Do not use coloured log outputs + nextflow_cli_args // array: List of positional nextflow CLI args + outdir // string: The output directory where the results will be saved + input // string: Path to input samplesheet + + main: + + ch_versions = Channel.empty() + + // + // Print version and exit if required and dump pipeline parameters to JSON file + // + UTILS_NEXTFLOW_PIPELINE ( + version, + true, + outdir, + workflow.profile.tokenize(',').intersect(['conda', 'mamba']).size() >= 1 + ) + + // + // Validate parameters and generate parameter summary to stdout + // + pre_help_text = nfCoreLogo(monochrome_logs) + post_help_text = '\n' + workflowCitation() + '\n' + dashedLine(monochrome_logs) + def String workflow_command = "nextflow run ${workflow.manifest.name} -profile --input samplesheet.csv --outdir " + UTILS_NFVALIDATION_PLUGIN ( + help, + workflow_command, + pre_help_text, + post_help_text, + validate_params, + "nextflow_schema.json" + ) + + // + // Check config provided to the pipeline + // + UTILS_NFCORE_PIPELINE ( + nextflow_cli_args + ) + // + // Custom validation for pipeline parameters + // + validateInputParameters() + + // + // Create channel from input file provided through params.input + // + Channel + .fromSamplesheet("input") + .map { + meta, fastq_1, fastq_2 -> + if (!fastq_2) { + return [ meta.id, meta + [ single_end:true ], [ fastq_1 ] ] + } else { + return [ meta.id, meta + [ single_end:false ], [ fastq_1, fastq_2 ] ] + } + } + .groupTuple() + .map { + validateInputSamplesheet(it) + } + .map { + meta, fastqs -> + return [ meta, fastqs.flatten() ] + } + .set { ch_samplesheet } + + emit: + samplesheet = ch_samplesheet + versions = ch_versions +} + +/* +======================================================================================== + SUBWORKFLOW FOR PIPELINE COMPLETION +======================================================================================== +*/ + +workflow PIPELINE_COMPLETION { + + take: + email // string: email address + email_on_fail // string: email address sent on pipeline failure + plaintext_email // boolean: Send plain-text email instead of HTML + outdir // path: Path to output directory where results will be published + monochrome_logs // boolean: Disable ANSI colour codes in log output + hook_url // string: hook URL for notifications + multiqc_report // string: Path to MultiQC report + + main: + + summary_params = paramsSummaryMap(workflow, parameters_schema: "nextflow_schema.json") + + // + // Completion email and summary + // + workflow.onComplete { + if (email || email_on_fail) { + completionEmail(summary_params, email, email_on_fail, plaintext_email, outdir, monochrome_logs, multiqc_report.toList()) + } + + completionSummary(monochrome_logs) + + if (hook_url) { + imNotification(summary_params, hook_url) + } + } +} + +/* +======================================================================================== + FUNCTIONS +======================================================================================== +*/ +// +// Check and validate pipeline parameters +// +def validateInputParameters() { + genomeExistsError() +} + +// +// Validate channels from input samplesheet +// +def validateInputSamplesheet(input) { + def (metas, fastqs) = input[1..2] + + // Check that multiple runs of the same sample are of the same datatype i.e. single-end / paired-end + def endedness_ok = metas.collect{ it.single_end }.unique().size == 1 + if (!endedness_ok) { + error("Please check input samplesheet -> Multiple runs of a sample must be of the same datatype i.e. single-end or paired-end: ${metas[0].id}") + } + + return [ metas[0], fastqs ] +} +// +// Get attribute from genome config file e.g. fasta +// +def getGenomeAttribute(attribute) { + if (params.genomes && params.genome && params.genomes.containsKey(params.genome)) { + if (params.genomes[ params.genome ].containsKey(attribute)) { + return params.genomes[ params.genome ][ attribute ] + } + } + return null +} + +// +// Exit pipeline if incorrect --genome key provided +// +def genomeExistsError() { + if (params.genomes && params.genome && !params.genomes.containsKey(params.genome)) { + def error_string = "~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~\n" + + " Genome '${params.genome}' not found in any config files provided to the pipeline.\n" + + " Currently, the available genome keys are:\n" + + " ${params.genomes.keySet().join(", ")}\n" + + "~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~" + error(error_string) + } +} + +// +// Generate methods description for MultiQC +// +def toolCitationText() { + // TODO nf-core: Optionally add in-text citation tools to this list. + // Can use ternary operators to dynamically construct based conditions, e.g. params["run_xyz"] ? "Tool (Foo et al. 2023)" : "", + // Uncomment function in methodsDescriptionText to render in MultiQC report + def citation_text = [ + "Tools used in the workflow included:", + "FastQC (Andrews 2010),", + "MultiQC (Ewels et al. 2016)", + "." + ].join(' ').trim() + + return citation_text +} + +def toolBibliographyText() { + // TODO nf-core: Optionally add bibliographic entries to this list. + // Can use ternary operators to dynamically construct based conditions, e.g. params["run_xyz"] ? "
  • Author (2023) Pub name, Journal, DOI
    • " : "", + // Uncomment function in methodsDescriptionText to render in MultiQC report + def reference_text = [ + "
  • Andrews S, (2010) FastQC, URL: https://www.bioinformatics.babraham.ac.uk/projects/fastqc/).
    • ", + "
  • Ewels, P., Magnusson, M., Lundin, S., & Käller, M. (2016). MultiQC: summarize analysis results for multiple tools and samples in a single report. Bioinformatics , 32(19), 3047–3048. doi: /10.1093/bioinformatics/btw354
    • " + ].join(' ').trim() + + return reference_text +} + +def methodsDescriptionText(mqc_methods_yaml) { + // Convert to a named map so can be used as with familar NXF ${workflow} variable syntax in the MultiQC YML file + def meta = [:] + meta.workflow = workflow.toMap() + meta["manifest_map"] = workflow.manifest.toMap() + + // Pipeline DOI + meta["doi_text"] = meta.manifest_map.doi ? "(doi:     ${meta.manifest_map.doi})" : "" + meta["nodoi_text"] = meta.manifest_map.doi ? "": "
  • If available, make sure to update the text to include the Zenodo DOI of version of the pipeline used.
    • " + + // Tool references + meta["tool_citations"] = "" + meta["tool_bibliography"] = "" + + // TODO nf-core: Only uncomment below if logic in toolCitationText/toolBibliographyText has been filled! + // meta["tool_citations"] = toolCitationText().replaceAll(", \\.", ".").replaceAll("\\. \\.", ".").replaceAll(", \\.", ".") + // meta["tool_bibliography"] = toolBibliographyText() + + + def methods_text = mqc_methods_yaml.text + + def engine = new groovy.text.SimpleTemplateEngine() + def description_html = engine.createTemplate(methods_text).make(meta) + + return description_html.toString() +} diff --git a/subworkflows/nf-core/utils_nextflow_pipeline/main.nf b/subworkflows/nf-core/utils_nextflow_pipeline/main.nf new file mode 100644 index 0000000..ac31f28 --- /dev/null +++ b/subworkflows/nf-core/utils_nextflow_pipeline/main.nf @@ -0,0 +1,126 @@ +// +// Subworkflow with functionality that may be useful for any Nextflow pipeline +// + +import org.yaml.snakeyaml.Yaml +import groovy.json.JsonOutput +import nextflow.extension.FilesEx + +/* +======================================================================================== + SUBWORKFLOW DEFINITION +======================================================================================== +*/ + +workflow UTILS_NEXTFLOW_PIPELINE { + + take: + print_version // boolean: print version + dump_parameters // boolean: dump parameters + outdir // path: base directory used to publish pipeline results + check_conda_channels // boolean: check conda channels + + main: + + // + // Print workflow version and exit on --version + // + if (print_version) { + log.info "${workflow.manifest.name} ${getWorkflowVersion()}" + System.exit(0) + } + + // + // Dump pipeline parameters to a JSON file + // + if (dump_parameters && outdir) { + dumpParametersToJSON(outdir) + } + + // + // When running with Conda, warn if channels have not been set-up appropriately + // + if (check_conda_channels) { + checkCondaChannels() + } + + emit: + dummy_emit = true +} + +/* +======================================================================================== + FUNCTIONS +======================================================================================== +*/ + +// +// Generate version string +// +def getWorkflowVersion() { + String version_string = "" + if (workflow.manifest.version) { + def prefix_v = workflow.manifest.version[0] != 'v' ? 'v' : '' + version_string += "${prefix_v}${workflow.manifest.version}" + } + + if (workflow.commitId) { + def git_shortsha = workflow.commitId.substring(0, 7) + version_string += "-g${git_shortsha}" + } + + return version_string +} + +// +// Dump pipeline parameters to a JSON file +// +def dumpParametersToJSON(outdir) { + def timestamp = new java.util.Date().format( 'yyyy-MM-dd_HH-mm-ss') + def filename = "params_${timestamp}.json" + def temp_pf = new File(workflow.launchDir.toString(), ".${filename}") + def jsonStr = JsonOutput.toJson(params) + temp_pf.text = JsonOutput.prettyPrint(jsonStr) + + FilesEx.copyTo(temp_pf.toPath(), "${outdir}/pipeline_info/params_${timestamp}.json") + temp_pf.delete() +} + +// +// When running with -profile conda, warn if channels have not been set-up appropriately +// +def checkCondaChannels() { + Yaml parser = new Yaml() + def channels = [] + try { + def config = parser.load("conda config --show channels".execute().text) + channels = config.channels + } catch(NullPointerException | IOException e) { + log.warn "Could not verify conda channel configuration." + return + } + + // Check that all channels are present + // This channel list is ordered by required channel priority. + def required_channels_in_order = ['conda-forge', 'bioconda', 'defaults'] + def channels_missing = ((required_channels_in_order as Set) - (channels as Set)) as Boolean + + // Check that they are in the right order + def channel_priority_violation = false + def n = required_channels_in_order.size() + for (int i = 0; i < n - 1; i++) { + channel_priority_violation |= !(channels.indexOf(required_channels_in_order[i]) < channels.indexOf(required_channels_in_order[i+1])) + } + + if (channels_missing | channel_priority_violation) { + log.warn "~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~\n" + + " There is a problem with your Conda configuration!\n\n" + + " You will need to set-up the conda-forge and bioconda channels correctly.\n" + + " Please refer to https://bioconda.github.io/\n" + + " The observed channel order is \n" + + " ${channels}\n" + + " but the following channel order is required:\n" + + " ${required_channels_in_order}\n" + + "~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~" + } +} diff --git a/subworkflows/nf-core/utils_nextflow_pipeline/meta.yml b/subworkflows/nf-core/utils_nextflow_pipeline/meta.yml new file mode 100644 index 0000000..e5c3a0a --- /dev/null +++ b/subworkflows/nf-core/utils_nextflow_pipeline/meta.yml @@ -0,0 +1,38 @@ +# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/subworkflows/yaml-schema.json +name: "UTILS_NEXTFLOW_PIPELINE" +description: Subworkflow with functionality that may be useful for any Nextflow pipeline +keywords: + - utility + - pipeline + - initialise + - version +components: [] +input: + - print_version: + type: boolean + description: | + Print the version of the pipeline and exit + - dump_parameters: + type: boolean + description: | + Dump the parameters of the pipeline to a JSON file + - output_directory: + type: directory + description: Path to output dir to write JSON file to. + pattern: "results/" + - check_conda_channel: + type: boolean + description: | + Check if the conda channel priority is correct. +output: + - dummy_emit: + type: boolean + description: | + Dummy emit to make nf-core subworkflows lint happy +authors: + - "@adamrtalbot" + - "@drpatelh" +maintainers: + - "@adamrtalbot" + - "@drpatelh" + - "@maxulysse" diff --git a/subworkflows/nf-core/utils_nextflow_pipeline/tests/main.function.nf.test b/subworkflows/nf-core/utils_nextflow_pipeline/tests/main.function.nf.test new file mode 100644 index 0000000..68718e4 --- /dev/null +++ b/subworkflows/nf-core/utils_nextflow_pipeline/tests/main.function.nf.test @@ -0,0 +1,54 @@ + +nextflow_function { + + name "Test Functions" + script "subworkflows/nf-core/utils_nextflow_pipeline/main.nf" + config "subworkflows/nf-core/utils_nextflow_pipeline/tests/nextflow.config" + tag 'subworkflows' + tag 'utils_nextflow_pipeline' + tag 'subworkflows/utils_nextflow_pipeline' + + test("Test Function getWorkflowVersion") { + + function "getWorkflowVersion" + + then { + assertAll( + { assert function.success }, + { assert snapshot(function.result).match() } + ) + } + } + + test("Test Function dumpParametersToJSON") { + + function "dumpParametersToJSON" + + when { + function { + """ + // define inputs of the function here. Example: + input[0] = "$outputDir" + """.stripIndent() + } + } + + then { + assertAll( + { assert function.success } + ) + } + } + + test("Test Function checkCondaChannels") { + + function "checkCondaChannels" + + then { + assertAll( + { assert function.success }, + { assert snapshot(function.result).match() } + ) + } + } +} diff --git a/subworkflows/nf-core/utils_nextflow_pipeline/tests/main.function.nf.test.snap b/subworkflows/nf-core/utils_nextflow_pipeline/tests/main.function.nf.test.snap new file mode 100644 index 0000000..e3f0baf --- /dev/null +++ b/subworkflows/nf-core/utils_nextflow_pipeline/tests/main.function.nf.test.snap @@ -0,0 +1,20 @@ +{ + "Test Function getWorkflowVersion": { + "content": [ + "v9.9.9" + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-02-28T12:02:05.308243" + }, + "Test Function checkCondaChannels": { + "content": null, + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-02-28T12:02:12.425833" + } +} \ No newline at end of file diff --git a/subworkflows/nf-core/utils_nextflow_pipeline/tests/main.workflow.nf.test b/subworkflows/nf-core/utils_nextflow_pipeline/tests/main.workflow.nf.test new file mode 100644 index 0000000..ca964ce --- /dev/null +++ b/subworkflows/nf-core/utils_nextflow_pipeline/tests/main.workflow.nf.test @@ -0,0 +1,111 @@ +nextflow_workflow { + + name "Test Workflow UTILS_NEXTFLOW_PIPELINE" + script "../main.nf" + config "subworkflows/nf-core/utils_nextflow_pipeline/tests/nextflow.config" + workflow "UTILS_NEXTFLOW_PIPELINE" + tag 'subworkflows' + tag 'utils_nextflow_pipeline' + tag 'subworkflows/utils_nextflow_pipeline' + + test("Should run no inputs") { + + when { + workflow { + """ + print_version = false + dump_parameters = false + outdir = null + check_conda_channels = false + + input[0] = print_version + input[1] = dump_parameters + input[2] = outdir + input[3] = check_conda_channels + """ + } + } + + then { + assertAll( + { assert workflow.success } + ) + } + } + + test("Should print version") { + + when { + workflow { + """ + print_version = true + dump_parameters = false + outdir = null + check_conda_channels = false + + input[0] = print_version + input[1] = dump_parameters + input[2] = outdir + input[3] = check_conda_channels + """ + } + } + + then { + assertAll( + { assert workflow.success }, + { assert workflow.stdout.contains("nextflow_workflow v9.9.9") } + ) + } + } + + test("Should dump params") { + + when { + workflow { + """ + print_version = false + dump_parameters = true + outdir = 'results' + check_conda_channels = false + + input[0] = false + input[1] = true + input[2] = outdir + input[3] = false + """ + } + } + + then { + assertAll( + { assert workflow.success } + ) + } + } + + test("Should not create params JSON if no output directory") { + + when { + workflow { + """ + print_version = false + dump_parameters = true + outdir = null + check_conda_channels = false + + input[0] = false + input[1] = true + input[2] = outdir + input[3] = false + """ + } + } + + then { + assertAll( + { assert workflow.success } + ) + } + } +} diff --git a/subworkflows/nf-core/utils_nextflow_pipeline/tests/nextflow.config b/subworkflows/nf-core/utils_nextflow_pipeline/tests/nextflow.config new file mode 100644 index 0000000..d0a926b --- /dev/null +++ b/subworkflows/nf-core/utils_nextflow_pipeline/tests/nextflow.config @@ -0,0 +1,9 @@ +manifest { + name = 'nextflow_workflow' + author = """nf-core""" + homePage = 'https://127.0.0.1' + description = """Dummy pipeline""" + nextflowVersion = '!>=23.04.0' + version = '9.9.9' + doi = 'https://doi.org/10.5281/zenodo.5070524' +} diff --git a/subworkflows/nf-core/utils_nextflow_pipeline/tests/tags.yml b/subworkflows/nf-core/utils_nextflow_pipeline/tests/tags.yml new file mode 100644 index 0000000..f847611 --- /dev/null +++ b/subworkflows/nf-core/utils_nextflow_pipeline/tests/tags.yml @@ -0,0 +1,2 @@ +subworkflows/utils_nextflow_pipeline: + - subworkflows/nf-core/utils_nextflow_pipeline/** diff --git a/subworkflows/nf-core/utils_nfcore_pipeline/main.nf b/subworkflows/nf-core/utils_nfcore_pipeline/main.nf new file mode 100644 index 0000000..a8b55d6 --- /dev/null +++ b/subworkflows/nf-core/utils_nfcore_pipeline/main.nf @@ -0,0 +1,440 @@ +// +// Subworkflow with utility functions specific to the nf-core pipeline template +// + +import org.yaml.snakeyaml.Yaml +import nextflow.extension.FilesEx + +/* +======================================================================================== + SUBWORKFLOW DEFINITION +======================================================================================== +*/ + +workflow UTILS_NFCORE_PIPELINE { + + take: + nextflow_cli_args + + main: + valid_config = checkConfigProvided() + checkProfileProvided(nextflow_cli_args) + + emit: + valid_config +} + +/* +======================================================================================== + FUNCTIONS +======================================================================================== +*/ + +// +// Warn if a -profile or Nextflow config has not been provided to run the pipeline +// +def checkConfigProvided() { + valid_config = true + if (workflow.profile == 'standard' && workflow.configFiles.size() <= 1) { + log.warn "[$workflow.manifest.name] You are attempting to run the pipeline without any custom configuration!\n\n" + + "This will be dependent on your local compute environment but can be achieved via one or more of the following:\n" + + " (1) Using an existing pipeline profile e.g. `-profile docker` or `-profile singularity`\n" + + " (2) Using an existing nf-core/configs for your Institution e.g. `-profile crick` or `-profile uppmax`\n" + + " (3) Using your own local custom config e.g. `-c /path/to/your/custom.config`\n\n" + + "Please refer to the quick start section and usage docs for the pipeline.\n " + valid_config = false + } + return valid_config +} + +// +// Exit pipeline if --profile contains spaces +// +def checkProfileProvided(nextflow_cli_args) { + if (workflow.profile.endsWith(',')) { + error "The `-profile` option cannot end with a trailing comma, please remove it and re-run the pipeline!\n" + + "HINT: A common mistake is to provide multiple values separated by spaces e.g. `-profile test, docker`.\n" + } + if (nextflow_cli_args[0]) { + log.warn "nf-core pipelines do not accept positional arguments. The positional argument `${nextflow_cli_args[0]}` has been detected.\n" + + "HINT: A common mistake is to provide multiple values separated by spaces e.g. `-profile test, docker`.\n" + } +} + +// +// Citation string for pipeline +// +def workflowCitation() { + return "If you use ${workflow.manifest.name} for your analysis please cite:\n\n" + + "* The pipeline\n" + + " ${workflow.manifest.doi}\n\n" + + "* The nf-core framework\n" + + " https://doi.org/10.1038/s41587-020-0439-x\n\n" + + "* Software dependencies\n" + + " https://github.com/${workflow.manifest.name}/blob/master/CITATIONS.md" +} + +// +// Generate workflow version string +// +def getWorkflowVersion() { + String version_string = "" + if (workflow.manifest.version) { + def prefix_v = workflow.manifest.version[0] != 'v' ? 'v' : '' + version_string += "${prefix_v}${workflow.manifest.version}" + } + + if (workflow.commitId) { + def git_shortsha = workflow.commitId.substring(0, 7) + version_string += "-g${git_shortsha}" + } + + return version_string +} + +// +// Get software versions for pipeline +// +def processVersionsFromYAML(yaml_file) { + Yaml yaml = new Yaml() + versions = yaml.load(yaml_file).collectEntries { k, v -> [ k.tokenize(':')[-1], v ] } + return yaml.dumpAsMap(versions).trim() +} + +// +// Get workflow version for pipeline +// +def workflowVersionToYAML() { + return """ + Workflow: + $workflow.manifest.name: ${getWorkflowVersion()} + Nextflow: $workflow.nextflow.version + """.stripIndent().trim() +} + +// +// Get channel of software versions used in pipeline in YAML format +// +def softwareVersionsToYAML(ch_versions) { + return ch_versions + .unique() + .map { processVersionsFromYAML(it) } + .unique() + .mix(Channel.of(workflowVersionToYAML())) +} + +// +// Get workflow summary for MultiQC +// +def paramsSummaryMultiqc(summary_params) { + def summary_section = '' + for (group in summary_params.keySet()) { + def group_params = summary_params.get(group) // This gets the parameters of that particular group + if (group_params) { + summary_section += "

    $group

    \n" + summary_section += "
    \n" + for (param in group_params.keySet()) { + summary_section += "
    $param
    ${group_params.get(param) ?: 'N/A'}
    \n" + } + summary_section += "
    \n" + } + } + + String yaml_file_text = "id: '${workflow.manifest.name.replace('/','-')}-summary'\n" + yaml_file_text += "description: ' - this information is collected when the pipeline is started.'\n" + yaml_file_text += "section_name: '${workflow.manifest.name} Workflow Summary'\n" + yaml_file_text += "section_href: 'https://github.com/${workflow.manifest.name}'\n" + yaml_file_text += "plot_type: 'html'\n" + yaml_file_text += "data: |\n" + yaml_file_text += "${summary_section}" + + return yaml_file_text +} + +// +// nf-core logo +// +def nfCoreLogo(monochrome_logs=true) { + Map colors = logColours(monochrome_logs) + String.format( + """\n + ${dashedLine(monochrome_logs)} + ${colors.green},--.${colors.black}/${colors.green},-.${colors.reset} + ${colors.blue} ___ __ __ __ ___ ${colors.green}/,-._.--~\'${colors.reset} + ${colors.blue} |\\ | |__ __ / ` / \\ |__) |__ ${colors.yellow}} {${colors.reset} + ${colors.blue} | \\| | \\__, \\__/ | \\ |___ ${colors.green}\\`-._,-`-,${colors.reset} + ${colors.green}`._,._,\'${colors.reset} + ${colors.purple} ${workflow.manifest.name} ${getWorkflowVersion()}${colors.reset} + ${dashedLine(monochrome_logs)} + """.stripIndent() + ) +} + +// +// Return dashed line +// +def dashedLine(monochrome_logs=true) { + Map colors = logColours(monochrome_logs) + return "-${colors.dim}----------------------------------------------------${colors.reset}-" +} + +// +// ANSII colours used for terminal logging +// +def logColours(monochrome_logs=true) { + Map colorcodes = [:] + + // Reset / Meta + colorcodes['reset'] = monochrome_logs ? '' : "\033[0m" + colorcodes['bold'] = monochrome_logs ? '' : "\033[1m" + colorcodes['dim'] = monochrome_logs ? '' : "\033[2m" + colorcodes['underlined'] = monochrome_logs ? '' : "\033[4m" + colorcodes['blink'] = monochrome_logs ? '' : "\033[5m" + colorcodes['reverse'] = monochrome_logs ? '' : "\033[7m" + colorcodes['hidden'] = monochrome_logs ? '' : "\033[8m" + + // Regular Colors + colorcodes['black'] = monochrome_logs ? '' : "\033[0;30m" + colorcodes['red'] = monochrome_logs ? '' : "\033[0;31m" + colorcodes['green'] = monochrome_logs ? '' : "\033[0;32m" + colorcodes['yellow'] = monochrome_logs ? '' : "\033[0;33m" + colorcodes['blue'] = monochrome_logs ? '' : "\033[0;34m" + colorcodes['purple'] = monochrome_logs ? '' : "\033[0;35m" + colorcodes['cyan'] = monochrome_logs ? '' : "\033[0;36m" + colorcodes['white'] = monochrome_logs ? '' : "\033[0;37m" + + // Bold + colorcodes['bblack'] = monochrome_logs ? '' : "\033[1;30m" + colorcodes['bred'] = monochrome_logs ? '' : "\033[1;31m" + colorcodes['bgreen'] = monochrome_logs ? '' : "\033[1;32m" + colorcodes['byellow'] = monochrome_logs ? '' : "\033[1;33m" + colorcodes['bblue'] = monochrome_logs ? '' : "\033[1;34m" + colorcodes['bpurple'] = monochrome_logs ? '' : "\033[1;35m" + colorcodes['bcyan'] = monochrome_logs ? '' : "\033[1;36m" + colorcodes['bwhite'] = monochrome_logs ? '' : "\033[1;37m" + + // Underline + colorcodes['ublack'] = monochrome_logs ? '' : "\033[4;30m" + colorcodes['ured'] = monochrome_logs ? '' : "\033[4;31m" + colorcodes['ugreen'] = monochrome_logs ? '' : "\033[4;32m" + colorcodes['uyellow'] = monochrome_logs ? '' : "\033[4;33m" + colorcodes['ublue'] = monochrome_logs ? '' : "\033[4;34m" + colorcodes['upurple'] = monochrome_logs ? '' : "\033[4;35m" + colorcodes['ucyan'] = monochrome_logs ? '' : "\033[4;36m" + colorcodes['uwhite'] = monochrome_logs ? '' : "\033[4;37m" + + // High Intensity + colorcodes['iblack'] = monochrome_logs ? '' : "\033[0;90m" + colorcodes['ired'] = monochrome_logs ? '' : "\033[0;91m" + colorcodes['igreen'] = monochrome_logs ? '' : "\033[0;92m" + colorcodes['iyellow'] = monochrome_logs ? '' : "\033[0;93m" + colorcodes['iblue'] = monochrome_logs ? '' : "\033[0;94m" + colorcodes['ipurple'] = monochrome_logs ? '' : "\033[0;95m" + colorcodes['icyan'] = monochrome_logs ? '' : "\033[0;96m" + colorcodes['iwhite'] = monochrome_logs ? '' : "\033[0;97m" + + // Bold High Intensity + colorcodes['biblack'] = monochrome_logs ? '' : "\033[1;90m" + colorcodes['bired'] = monochrome_logs ? '' : "\033[1;91m" + colorcodes['bigreen'] = monochrome_logs ? '' : "\033[1;92m" + colorcodes['biyellow'] = monochrome_logs ? '' : "\033[1;93m" + colorcodes['biblue'] = monochrome_logs ? '' : "\033[1;94m" + colorcodes['bipurple'] = monochrome_logs ? '' : "\033[1;95m" + colorcodes['bicyan'] = monochrome_logs ? '' : "\033[1;96m" + colorcodes['biwhite'] = monochrome_logs ? '' : "\033[1;97m" + + return colorcodes +} + +// +// Attach the multiqc report to email +// +def attachMultiqcReport(multiqc_report) { + def mqc_report = null + try { + if (workflow.success) { + mqc_report = multiqc_report.getVal() + if (mqc_report.getClass() == ArrayList && mqc_report.size() >= 1) { + if (mqc_report.size() > 1) { + log.warn "[$workflow.manifest.name] Found multiple reports from process 'MULTIQC', will use only one" + } + mqc_report = mqc_report[0] + } + } + } catch (all) { + if (multiqc_report) { + log.warn "[$workflow.manifest.name] Could not attach MultiQC report to summary email" + } + } + return mqc_report +} + +// +// Construct and send completion email +// +def completionEmail(summary_params, email, email_on_fail, plaintext_email, outdir, monochrome_logs=true, multiqc_report=null) { + + // Set up the e-mail variables + def subject = "[$workflow.manifest.name] Successful: $workflow.runName" + if (!workflow.success) { + subject = "[$workflow.manifest.name] FAILED: $workflow.runName" + } + + def summary = [:] + for (group in summary_params.keySet()) { + summary << summary_params[group] + } + + def misc_fields = [:] + misc_fields['Date Started'] = workflow.start + misc_fields['Date Completed'] = workflow.complete + misc_fields['Pipeline script file path'] = workflow.scriptFile + misc_fields['Pipeline script hash ID'] = workflow.scriptId + if (workflow.repository) misc_fields['Pipeline repository Git URL'] = workflow.repository + if (workflow.commitId) misc_fields['Pipeline repository Git Commit'] = workflow.commitId + if (workflow.revision) misc_fields['Pipeline Git branch/tag'] = workflow.revision + misc_fields['Nextflow Version'] = workflow.nextflow.version + misc_fields['Nextflow Build'] = workflow.nextflow.build + misc_fields['Nextflow Compile Timestamp'] = workflow.nextflow.timestamp + + def email_fields = [:] + email_fields['version'] = getWorkflowVersion() + email_fields['runName'] = workflow.runName + email_fields['success'] = workflow.success + email_fields['dateComplete'] = workflow.complete + email_fields['duration'] = workflow.duration + email_fields['exitStatus'] = workflow.exitStatus + email_fields['errorMessage'] = (workflow.errorMessage ?: 'None') + email_fields['errorReport'] = (workflow.errorReport ?: 'None') + email_fields['commandLine'] = workflow.commandLine + email_fields['projectDir'] = workflow.projectDir + email_fields['summary'] = summary << misc_fields + + // On success try attach the multiqc report + def mqc_report = attachMultiqcReport(multiqc_report) + + // Check if we are only sending emails on failure + def email_address = email + if (!email && email_on_fail && !workflow.success) { + email_address = email_on_fail + } + + // Render the TXT template + def engine = new groovy.text.GStringTemplateEngine() + def tf = new File("${workflow.projectDir}/assets/email_template.txt") + def txt_template = engine.createTemplate(tf).make(email_fields) + def email_txt = txt_template.toString() + + // Render the HTML template + def hf = new File("${workflow.projectDir}/assets/email_template.html") + def html_template = engine.createTemplate(hf).make(email_fields) + def email_html = html_template.toString() + + // Render the sendmail template + def max_multiqc_email_size = (params.containsKey('max_multiqc_email_size') ? params.max_multiqc_email_size : 0) as nextflow.util.MemoryUnit + def smail_fields = [ email: email_address, subject: subject, email_txt: email_txt, email_html: email_html, projectDir: "${workflow.projectDir}", mqcFile: mqc_report, mqcMaxSize: max_multiqc_email_size.toBytes() ] + def sf = new File("${workflow.projectDir}/assets/sendmail_template.txt") + def sendmail_template = engine.createTemplate(sf).make(smail_fields) + def sendmail_html = sendmail_template.toString() + + // Send the HTML e-mail + Map colors = logColours(monochrome_logs) + if (email_address) { + try { + if (plaintext_email) { throw GroovyException('Send plaintext e-mail, not HTML') } + // Try to send HTML e-mail using sendmail + def sendmail_tf = new File(workflow.launchDir.toString(), ".sendmail_tmp.html") + sendmail_tf.withWriter { w -> w << sendmail_html } + [ 'sendmail', '-t' ].execute() << sendmail_html + log.info "-${colors.purple}[$workflow.manifest.name]${colors.green} Sent summary e-mail to $email_address (sendmail)-" + } catch (all) { + // Catch failures and try with plaintext + def mail_cmd = [ 'mail', '-s', subject, '--content-type=text/html', email_address ] + mail_cmd.execute() << email_html + log.info "-${colors.purple}[$workflow.manifest.name]${colors.green} Sent summary e-mail to $email_address (mail)-" + } + } + + // Write summary e-mail HTML to a file + def output_hf = new File(workflow.launchDir.toString(), ".pipeline_report.html") + output_hf.withWriter { w -> w << email_html } + FilesEx.copyTo(output_hf.toPath(), "${outdir}/pipeline_info/pipeline_report.html"); + output_hf.delete() + + // Write summary e-mail TXT to a file + def output_tf = new File(workflow.launchDir.toString(), ".pipeline_report.txt") + output_tf.withWriter { w -> w << email_txt } + FilesEx.copyTo(output_tf.toPath(), "${outdir}/pipeline_info/pipeline_report.txt"); + output_tf.delete() +} + +// +// Print pipeline summary on completion +// +def completionSummary(monochrome_logs=true) { + Map colors = logColours(monochrome_logs) + if (workflow.success) { + if (workflow.stats.ignoredCount == 0) { + log.info "-${colors.purple}[$workflow.manifest.name]${colors.green} Pipeline completed successfully${colors.reset}-" + } else { + log.info "-${colors.purple}[$workflow.manifest.name]${colors.yellow} Pipeline completed successfully, but with errored process(es) ${colors.reset}-" + } + } else { + log.info "-${colors.purple}[$workflow.manifest.name]${colors.red} Pipeline completed with errors${colors.reset}-" + } +} + +// +// Construct and send a notification to a web server as JSON e.g. Microsoft Teams and Slack +// +def imNotification(summary_params, hook_url) { + def summary = [:] + for (group in summary_params.keySet()) { + summary << summary_params[group] + } + + def misc_fields = [:] + misc_fields['start'] = workflow.start + misc_fields['complete'] = workflow.complete + misc_fields['scriptfile'] = workflow.scriptFile + misc_fields['scriptid'] = workflow.scriptId + if (workflow.repository) misc_fields['repository'] = workflow.repository + if (workflow.commitId) misc_fields['commitid'] = workflow.commitId + if (workflow.revision) misc_fields['revision'] = workflow.revision + misc_fields['nxf_version'] = workflow.nextflow.version + misc_fields['nxf_build'] = workflow.nextflow.build + misc_fields['nxf_timestamp'] = workflow.nextflow.timestamp + + def msg_fields = [:] + msg_fields['version'] = getWorkflowVersion() + msg_fields['runName'] = workflow.runName + msg_fields['success'] = workflow.success + msg_fields['dateComplete'] = workflow.complete + msg_fields['duration'] = workflow.duration + msg_fields['exitStatus'] = workflow.exitStatus + msg_fields['errorMessage'] = (workflow.errorMessage ?: 'None') + msg_fields['errorReport'] = (workflow.errorReport ?: 'None') + msg_fields['commandLine'] = workflow.commandLine.replaceFirst(/ +--hook_url +[^ ]+/, "") + msg_fields['projectDir'] = workflow.projectDir + msg_fields['summary'] = summary << misc_fields + + // Render the JSON template + def engine = new groovy.text.GStringTemplateEngine() + // Different JSON depending on the service provider + // Defaults to "Adaptive Cards" (https://adaptivecards.io), except Slack which has its own format + def json_path = hook_url.contains("hooks.slack.com") ? "slackreport.json" : "adaptivecard.json" + def hf = new File("${workflow.projectDir}/assets/${json_path}") + def json_template = engine.createTemplate(hf).make(msg_fields) + def json_message = json_template.toString() + + // POST + def post = new URL(hook_url).openConnection(); + post.setRequestMethod("POST") + post.setDoOutput(true) + post.setRequestProperty("Content-Type", "application/json") + post.getOutputStream().write(json_message.getBytes("UTF-8")); + def postRC = post.getResponseCode(); + if (! postRC.equals(200)) { + log.warn(post.getErrorStream().getText()); + } +} diff --git a/subworkflows/nf-core/utils_nfcore_pipeline/meta.yml b/subworkflows/nf-core/utils_nfcore_pipeline/meta.yml new file mode 100644 index 0000000..d08d243 --- /dev/null +++ b/subworkflows/nf-core/utils_nfcore_pipeline/meta.yml @@ -0,0 +1,24 @@ +# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/subworkflows/yaml-schema.json +name: "UTILS_NFCORE_PIPELINE" +description: Subworkflow with utility functions specific to the nf-core pipeline template +keywords: + - utility + - pipeline + - initialise + - version +components: [] +input: + - nextflow_cli_args: + type: list + description: | + Nextflow CLI positional arguments +output: + - success: + type: boolean + description: | + Dummy output to indicate success +authors: + - "@adamrtalbot" +maintainers: + - "@adamrtalbot" + - "@maxulysse" diff --git a/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.function.nf.test b/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.function.nf.test new file mode 100644 index 0000000..1dc317f --- /dev/null +++ b/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.function.nf.test @@ -0,0 +1,134 @@ + +nextflow_function { + + name "Test Functions" + script "../main.nf" + config "subworkflows/nf-core/utils_nfcore_pipeline/tests/nextflow.config" + tag "subworkflows" + tag "subworkflows_nfcore" + tag "utils_nfcore_pipeline" + tag "subworkflows/utils_nfcore_pipeline" + + test("Test Function checkConfigProvided") { + + function "checkConfigProvided" + + then { + assertAll( + { assert function.success }, + { assert snapshot(function.result).match() } + ) + } + } + + test("Test Function checkProfileProvided") { + + function "checkProfileProvided" + + when { + function { + """ + input[0] = [] + """ + } + } + + then { + assertAll( + { assert function.success }, + { assert snapshot(function.result).match() } + ) + } + } + + test("Test Function workflowCitation") { + + function "workflowCitation" + + then { + assertAll( + { assert function.success }, + { assert snapshot(function.result).match() } + ) + } + } + + test("Test Function nfCoreLogo") { + + function "nfCoreLogo" + + when { + function { + """ + input[0] = false + """ + } + } + + then { + assertAll( + { assert function.success }, + { assert snapshot(function.result).match() } + ) + } + } + + test("Test Function dashedLine") { + + function "dashedLine" + + when { + function { + """ + input[0] = false + """ + } + } + + then { + assertAll( + { assert function.success }, + { assert snapshot(function.result).match() } + ) + } + } + + test("Test Function without logColours") { + + function "logColours" + + when { + function { + """ + input[0] = true + """ + } + } + + then { + assertAll( + { assert function.success }, + { assert snapshot(function.result).match() } + ) + } + } + + test("Test Function with logColours") { + function "logColours" + + when { + function { + """ + input[0] = false + """ + } + } + + then { + assertAll( + { assert function.success }, + { assert snapshot(function.result).match() } + ) + } + } +} diff --git a/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.function.nf.test.snap b/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.function.nf.test.snap new file mode 100644 index 0000000..1037232 --- /dev/null +++ b/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.function.nf.test.snap @@ -0,0 +1,166 @@ +{ + "Test Function checkProfileProvided": { + "content": null, + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-02-28T12:03:03.360873" + }, + "Test Function checkConfigProvided": { + "content": [ + true + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-02-28T12:02:59.729647" + }, + "Test Function nfCoreLogo": { + "content": [ + "\n\n-\u001b[2m----------------------------------------------------\u001b[0m-\n \u001b[0;32m,--.\u001b[0;30m/\u001b[0;32m,-.\u001b[0m\n\u001b[0;34m ___ __ __ __ ___ \u001b[0;32m/,-._.--~'\u001b[0m\n\u001b[0;34m |\\ | |__ __ / ` / \\ |__) |__ \u001b[0;33m} {\u001b[0m\n\u001b[0;34m | \\| | \\__, \\__/ | \\ |___ \u001b[0;32m\\`-._,-`-,\u001b[0m\n \u001b[0;32m`._,._,'\u001b[0m\n\u001b[0;35m nextflow_workflow v9.9.9\u001b[0m\n-\u001b[2m----------------------------------------------------\u001b[0m-\n" + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-02-28T12:03:10.562934" + }, + "Test Function workflowCitation": { + "content": [ + "If you use nextflow_workflow for your analysis please cite:\n\n* The pipeline\n https://doi.org/10.5281/zenodo.5070524\n\n* The nf-core framework\n https://doi.org/10.1038/s41587-020-0439-x\n\n* Software dependencies\n https://github.com/nextflow_workflow/blob/master/CITATIONS.md" + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-02-28T12:03:07.019761" + }, + "Test Function without logColours": { + "content": [ + { + "reset": "", + "bold": "", + "dim": "", + "underlined": "", + "blink": "", + "reverse": "", + "hidden": "", + "black": "", + "red": "", + "green": "", + "yellow": "", + "blue": "", + "purple": "", + "cyan": "", + "white": "", + "bblack": "", + "bred": "", + "bgreen": "", + "byellow": "", + "bblue": "", + "bpurple": "", + "bcyan": "", + "bwhite": "", + "ublack": "", + "ured": "", + "ugreen": "", + "uyellow": "", + "ublue": "", + "upurple": "", + "ucyan": "", + "uwhite": "", + "iblack": "", + "ired": "", + "igreen": "", + "iyellow": "", + "iblue": "", + "ipurple": "", + "icyan": "", + "iwhite": "", + "biblack": "", + "bired": "", + "bigreen": "", + "biyellow": "", + "biblue": "", + "bipurple": "", + "bicyan": "", + "biwhite": "" + } + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-02-28T12:03:17.969323" + }, + "Test Function dashedLine": { + "content": [ + "-\u001b[2m----------------------------------------------------\u001b[0m-" + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-02-28T12:03:14.366181" + }, + "Test Function with logColours": { + "content": [ + { + "reset": "\u001b[0m", + "bold": "\u001b[1m", + "dim": "\u001b[2m", + "underlined": "\u001b[4m", + "blink": "\u001b[5m", + "reverse": "\u001b[7m", + "hidden": "\u001b[8m", + "black": "\u001b[0;30m", + "red": "\u001b[0;31m", + "green": "\u001b[0;32m", + "yellow": "\u001b[0;33m", + "blue": "\u001b[0;34m", + "purple": "\u001b[0;35m", + "cyan": "\u001b[0;36m", + "white": "\u001b[0;37m", + "bblack": "\u001b[1;30m", + "bred": "\u001b[1;31m", + "bgreen": "\u001b[1;32m", + "byellow": "\u001b[1;33m", + "bblue": "\u001b[1;34m", + "bpurple": "\u001b[1;35m", + "bcyan": "\u001b[1;36m", + "bwhite": "\u001b[1;37m", + "ublack": "\u001b[4;30m", + "ured": "\u001b[4;31m", + "ugreen": "\u001b[4;32m", + "uyellow": "\u001b[4;33m", + "ublue": "\u001b[4;34m", + "upurple": "\u001b[4;35m", + "ucyan": "\u001b[4;36m", + "uwhite": "\u001b[4;37m", + "iblack": "\u001b[0;90m", + "ired": "\u001b[0;91m", + "igreen": "\u001b[0;92m", + "iyellow": "\u001b[0;93m", + "iblue": "\u001b[0;94m", + "ipurple": "\u001b[0;95m", + "icyan": "\u001b[0;96m", + "iwhite": "\u001b[0;97m", + "biblack": "\u001b[1;90m", + "bired": "\u001b[1;91m", + "bigreen": "\u001b[1;92m", + "biyellow": "\u001b[1;93m", + "biblue": "\u001b[1;94m", + "bipurple": "\u001b[1;95m", + "bicyan": "\u001b[1;96m", + "biwhite": "\u001b[1;97m" + } + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-02-28T12:03:21.714424" + } +} \ No newline at end of file diff --git a/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.workflow.nf.test b/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.workflow.nf.test new file mode 100644 index 0000000..8940d32 --- /dev/null +++ b/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.workflow.nf.test @@ -0,0 +1,29 @@ +nextflow_workflow { + + name "Test Workflow UTILS_NFCORE_PIPELINE" + script "../main.nf" + config "subworkflows/nf-core/utils_nfcore_pipeline/tests/nextflow.config" + workflow "UTILS_NFCORE_PIPELINE" + tag "subworkflows" + tag "subworkflows_nfcore" + tag "utils_nfcore_pipeline" + tag "subworkflows/utils_nfcore_pipeline" + + test("Should run without failures") { + + when { + workflow { + """ + input[0] = [] + """ + } + } + + then { + assertAll( + { assert workflow.success }, + { assert snapshot(workflow.out).match() } + ) + } + } +} diff --git a/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.workflow.nf.test.snap b/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.workflow.nf.test.snap new file mode 100644 index 0000000..859d103 --- /dev/null +++ b/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.workflow.nf.test.snap @@ -0,0 +1,19 @@ +{ + "Should run without failures": { + "content": [ + { + "0": [ + true + ], + "valid_config": [ + true + ] + } + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-02-28T12:03:25.726491" + } +} \ No newline at end of file diff --git a/subworkflows/nf-core/utils_nfcore_pipeline/tests/nextflow.config b/subworkflows/nf-core/utils_nfcore_pipeline/tests/nextflow.config new file mode 100644 index 0000000..d0a926b --- /dev/null +++ b/subworkflows/nf-core/utils_nfcore_pipeline/tests/nextflow.config @@ -0,0 +1,9 @@ +manifest { + name = 'nextflow_workflow' + author = """nf-core""" + homePage = 'https://127.0.0.1' + description = """Dummy pipeline""" + nextflowVersion = '!>=23.04.0' + version = '9.9.9' + doi = 'https://doi.org/10.5281/zenodo.5070524' +} diff --git a/subworkflows/nf-core/utils_nfcore_pipeline/tests/tags.yml b/subworkflows/nf-core/utils_nfcore_pipeline/tests/tags.yml new file mode 100644 index 0000000..ac8523c --- /dev/null +++ b/subworkflows/nf-core/utils_nfcore_pipeline/tests/tags.yml @@ -0,0 +1,2 @@ +subworkflows/utils_nfcore_pipeline: + - subworkflows/nf-core/utils_nfcore_pipeline/** diff --git a/subworkflows/nf-core/utils_nfvalidation_plugin/main.nf b/subworkflows/nf-core/utils_nfvalidation_plugin/main.nf new file mode 100644 index 0000000..2585b65 --- /dev/null +++ b/subworkflows/nf-core/utils_nfvalidation_plugin/main.nf @@ -0,0 +1,62 @@ +// +// Subworkflow that uses the nf-validation plugin to render help text and parameter summary +// + +/* +======================================================================================== + IMPORT NF-VALIDATION PLUGIN +======================================================================================== +*/ + +include { paramsHelp } from 'plugin/nf-validation' +include { paramsSummaryLog } from 'plugin/nf-validation' +include { validateParameters } from 'plugin/nf-validation' + +/* +======================================================================================== + SUBWORKFLOW DEFINITION +======================================================================================== +*/ + +workflow UTILS_NFVALIDATION_PLUGIN { + + take: + print_help // boolean: print help + workflow_command // string: default commmand used to run pipeline + pre_help_text // string: string to be printed before help text and summary log + post_help_text // string: string to be printed after help text and summary log + validate_params // boolean: validate parameters + schema_filename // path: JSON schema file, null to use default value + + main: + + log.debug "Using schema file: ${schema_filename}" + + // Default values for strings + pre_help_text = pre_help_text ?: '' + post_help_text = post_help_text ?: '' + workflow_command = workflow_command ?: '' + + // + // Print help message if needed + // + if (print_help) { + log.info pre_help_text + paramsHelp(workflow_command, parameters_schema: schema_filename) + post_help_text + System.exit(0) + } + + // + // Print parameter summary to stdout + // + log.info pre_help_text + paramsSummaryLog(workflow, parameters_schema: schema_filename) + post_help_text + + // + // Validate parameters relative to the parameter JSON schema + // + if (validate_params){ + validateParameters(parameters_schema: schema_filename) + } + + emit: + dummy_emit = true +} diff --git a/subworkflows/nf-core/utils_nfvalidation_plugin/meta.yml b/subworkflows/nf-core/utils_nfvalidation_plugin/meta.yml new file mode 100644 index 0000000..3d4a6b0 --- /dev/null +++ b/subworkflows/nf-core/utils_nfvalidation_plugin/meta.yml @@ -0,0 +1,44 @@ +# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/subworkflows/yaml-schema.json +name: "UTILS_NFVALIDATION_PLUGIN" +description: Use nf-validation to initiate and validate a pipeline +keywords: + - utility + - pipeline + - initialise + - validation +components: [] +input: + - print_help: + type: boolean + description: | + Print help message and exit + - workflow_command: + type: string + description: | + The command to run the workflow e.g. "nextflow run main.nf" + - pre_help_text: + type: string + description: | + Text to print before the help message + - post_help_text: + type: string + description: | + Text to print after the help message + - validate_params: + type: boolean + description: | + Validate the parameters and error if invalid. + - schema_filename: + type: string + description: | + The filename of the schema to validate against. +output: + - dummy_emit: + type: boolean + description: | + Dummy emit to make nf-core subworkflows lint happy +authors: + - "@adamrtalbot" +maintainers: + - "@adamrtalbot" + - "@maxulysse" diff --git a/subworkflows/nf-core/utils_nfvalidation_plugin/tests/main.nf.test b/subworkflows/nf-core/utils_nfvalidation_plugin/tests/main.nf.test new file mode 100644 index 0000000..5784a33 --- /dev/null +++ b/subworkflows/nf-core/utils_nfvalidation_plugin/tests/main.nf.test @@ -0,0 +1,200 @@ +nextflow_workflow { + + name "Test Workflow UTILS_NFVALIDATION_PLUGIN" + script "../main.nf" + workflow "UTILS_NFVALIDATION_PLUGIN" + tag "subworkflows" + tag "subworkflows_nfcore" + tag "plugin/nf-validation" + tag "'plugin/nf-validation'" + tag "utils_nfvalidation_plugin" + tag "subworkflows/utils_nfvalidation_plugin" + + test("Should run nothing") { + + when { + + params { + monochrome_logs = true + test_data = '' + } + + workflow { + """ + help = false + workflow_command = null + pre_help_text = null + post_help_text = null + validate_params = false + schema_filename = "$moduleTestDir/nextflow_schema.json" + + input[0] = help + input[1] = workflow_command + input[2] = pre_help_text + input[3] = post_help_text + input[4] = validate_params + input[5] = schema_filename + """ + } + } + + then { + assertAll( + { assert workflow.success } + ) + } + } + + test("Should run help") { + + + when { + + params { + monochrome_logs = true + test_data = '' + } + workflow { + """ + help = true + workflow_command = null + pre_help_text = null + post_help_text = null + validate_params = false + schema_filename = "$moduleTestDir/nextflow_schema.json" + + input[0] = help + input[1] = workflow_command + input[2] = pre_help_text + input[3] = post_help_text + input[4] = validate_params + input[5] = schema_filename + """ + } + } + + then { + assertAll( + { assert workflow.success }, + { assert workflow.exitStatus == 0 }, + { assert workflow.stdout.any { it.contains('Input/output options') } }, + { assert workflow.stdout.any { it.contains('--outdir') } } + ) + } + } + + test("Should run help with command") { + + when { + + params { + monochrome_logs = true + test_data = '' + } + workflow { + """ + help = true + workflow_command = "nextflow run noorg/doesntexist" + pre_help_text = null + post_help_text = null + validate_params = false + schema_filename = "$moduleTestDir/nextflow_schema.json" + + input[0] = help + input[1] = workflow_command + input[2] = pre_help_text + input[3] = post_help_text + input[4] = validate_params + input[5] = schema_filename + """ + } + } + + then { + assertAll( + { assert workflow.success }, + { assert workflow.exitStatus == 0 }, + { assert workflow.stdout.any { it.contains('nextflow run noorg/doesntexist') } }, + { assert workflow.stdout.any { it.contains('Input/output options') } }, + { assert workflow.stdout.any { it.contains('--outdir') } } + ) + } + } + + test("Should run help with extra text") { + + + when { + + params { + monochrome_logs = true + test_data = '' + } + workflow { + """ + help = true + workflow_command = "nextflow run noorg/doesntexist" + pre_help_text = "pre-help-text" + post_help_text = "post-help-text" + validate_params = false + schema_filename = "$moduleTestDir/nextflow_schema.json" + + input[0] = help + input[1] = workflow_command + input[2] = pre_help_text + input[3] = post_help_text + input[4] = validate_params + input[5] = schema_filename + """ + } + } + + then { + assertAll( + { assert workflow.success }, + { assert workflow.exitStatus == 0 }, + { assert workflow.stdout.any { it.contains('pre-help-text') } }, + { assert workflow.stdout.any { it.contains('nextflow run noorg/doesntexist') } }, + { assert workflow.stdout.any { it.contains('Input/output options') } }, + { assert workflow.stdout.any { it.contains('--outdir') } }, + { assert workflow.stdout.any { it.contains('post-help-text') } } + ) + } + } + + test("Should validate params") { + + when { + + params { + monochrome_logs = true + test_data = '' + outdir = 1 + } + workflow { + """ + help = false + workflow_command = null + pre_help_text = null + post_help_text = null + validate_params = true + schema_filename = "$moduleTestDir/nextflow_schema.json" + + input[0] = help + input[1] = workflow_command + input[2] = pre_help_text + input[3] = post_help_text + input[4] = validate_params + input[5] = schema_filename + """ + } + } + + then { + assertAll( + { assert workflow.failed }, + { assert workflow.stdout.any { it.contains('ERROR ~ ERROR: Validation of pipeline parameters failed!') } } + ) + } + } +} diff --git a/subworkflows/nf-core/utils_nfvalidation_plugin/tests/nextflow_schema.json b/subworkflows/nf-core/utils_nfvalidation_plugin/tests/nextflow_schema.json new file mode 100644 index 0000000..7626c1c --- /dev/null +++ b/subworkflows/nf-core/utils_nfvalidation_plugin/tests/nextflow_schema.json @@ -0,0 +1,96 @@ +{ + "$schema": "http://json-schema.org/draft-07/schema", + "$id": "https://raw.githubusercontent.com/./master/nextflow_schema.json", + "title": ". pipeline parameters", + "description": "", + "type": "object", + "definitions": { + "input_output_options": { + "title": "Input/output options", + "type": "object", + "fa_icon": "fas fa-terminal", + "description": "Define where the pipeline should find input data and save output data.", + "required": ["outdir"], + "properties": { + "validate_params": { + "type": "boolean", + "description": "Validate parameters?", + "default": true, + "hidden": true + }, + "outdir": { + "type": "string", + "format": "directory-path", + "description": "The output directory where the results will be saved. You have to use absolute paths to storage on Cloud infrastructure.", + "fa_icon": "fas fa-folder-open" + }, + "test_data_base": { + "type": "string", + "default": "https://raw.githubusercontent.com/nf-core/test-datasets/modules", + "description": "Base for test data directory", + "hidden": true + }, + "test_data": { + "type": "string", + "description": "Fake test data param", + "hidden": true + } + } + }, + "generic_options": { + "title": "Generic options", + "type": "object", + "fa_icon": "fas fa-file-import", + "description": "Less common options for the pipeline, typically set in a config file.", + "help_text": "These options are common to all nf-core pipelines and allow you to customise some of the core preferences for how the pipeline runs.\n\nTypically these options would be set in a Nextflow config file loaded for all pipeline runs, such as `~/.nextflow/config`.", + "properties": { + "help": { + "type": "boolean", + "description": "Display help text.", + "fa_icon": "fas fa-question-circle", + "hidden": true + }, + "version": { + "type": "boolean", + "description": "Display version and exit.", + "fa_icon": "fas fa-question-circle", + "hidden": true + }, + "logo": { + "type": "boolean", + "default": true, + "description": "Display nf-core logo in console output.", + "fa_icon": "fas fa-image", + "hidden": true + }, + "singularity_pull_docker_container": { + "type": "boolean", + "description": "Pull Singularity container from Docker?", + "hidden": true + }, + "publish_dir_mode": { + "type": "string", + "default": "copy", + "description": "Method used to save pipeline results to output directory.", + "help_text": "The Nextflow `publishDir` option specifies which intermediate files should be saved to the output directory. This option tells the pipeline what method should be used to move these files. See [Nextflow docs](https://www.nextflow.io/docs/latest/process.html#publishdir) for details.", + "fa_icon": "fas fa-copy", + "enum": ["symlink", "rellink", "link", "copy", "copyNoFollow", "move"], + "hidden": true + }, + "monochrome_logs": { + "type": "boolean", + "description": "Use monochrome_logs", + "hidden": true + } + } + } + }, + "allOf": [ + { + "$ref": "#/definitions/input_output_options" + }, + { + "$ref": "#/definitions/generic_options" + } + ] +} diff --git a/subworkflows/nf-core/utils_nfvalidation_plugin/tests/tags.yml b/subworkflows/nf-core/utils_nfvalidation_plugin/tests/tags.yml new file mode 100644 index 0000000..60b1cff --- /dev/null +++ b/subworkflows/nf-core/utils_nfvalidation_plugin/tests/tags.yml @@ -0,0 +1,2 @@ +subworkflows/utils_nfvalidation_plugin: + - subworkflows/nf-core/utils_nfvalidation_plugin/** diff --git a/workflows/exomecnv.nf b/workflows/exomecnv.nf new file mode 100644 index 0000000..12ac0fd --- /dev/null +++ b/workflows/exomecnv.nf @@ -0,0 +1,76 @@ +/* +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ + IMPORT MODULES / SUBWORKFLOWS / FUNCTIONS +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ +*/ + +include { FASTQC } from '../modules/nf-core/fastqc/main' +include { MULTIQC } from '../modules/nf-core/multiqc/main' +include { paramsSummaryMap } from 'plugin/nf-validation' +include { paramsSummaryMultiqc } from '../subworkflows/nf-core/utils_nfcore_pipeline' +include { softwareVersionsToYAML } from '../subworkflows/nf-core/utils_nfcore_pipeline' +include { methodsDescriptionText } from '../subworkflows/local/utils_nfcore_exomecnv_pipeline' + +/* +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ + RUN MAIN WORKFLOW +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ +*/ + +workflow EXOMECNV { + + take: + ch_samplesheet // channel: samplesheet read in from --input + + main: + + ch_versions = Channel.empty() + ch_multiqc_files = Channel.empty() + + // + // MODULE: Run FastQC + // + FASTQC ( + ch_samplesheet + ) + ch_multiqc_files = ch_multiqc_files.mix(FASTQC.out.zip.collect{it[1]}) + ch_versions = ch_versions.mix(FASTQC.out.versions.first()) + + // + // Collate and save software versions + // + softwareVersionsToYAML(ch_versions) + .collectFile(storeDir: "${params.outdir}/pipeline_info", name: 'nf_core_pipeline_software_mqc_versions.yml', sort: true, newLine: true) + .set { ch_collated_versions } + + // + // MODULE: MultiQC + // + ch_multiqc_config = Channel.fromPath("$projectDir/assets/multiqc_config.yml", checkIfExists: true) + ch_multiqc_custom_config = params.multiqc_config ? Channel.fromPath(params.multiqc_config, checkIfExists: true) : Channel.empty() + ch_multiqc_logo = params.multiqc_logo ? Channel.fromPath(params.multiqc_logo, checkIfExists: true) : Channel.empty() + summary_params = paramsSummaryMap(workflow, parameters_schema: "nextflow_schema.json") + ch_workflow_summary = Channel.value(paramsSummaryMultiqc(summary_params)) + ch_multiqc_custom_methods_description = params.multiqc_methods_description ? file(params.multiqc_methods_description, checkIfExists: true) : file("$projectDir/assets/methods_description_template.yml", checkIfExists: true) + ch_methods_description = Channel.value(methodsDescriptionText(ch_multiqc_custom_methods_description)) + ch_multiqc_files = ch_multiqc_files.mix(ch_workflow_summary.collectFile(name: 'workflow_summary_mqc.yaml')) + ch_multiqc_files = ch_multiqc_files.mix(ch_collated_versions) + ch_multiqc_files = ch_multiqc_files.mix(ch_methods_description.collectFile(name: 'methods_description_mqc.yaml', sort: false)) + + MULTIQC ( + ch_multiqc_files.collect(), + ch_multiqc_config.toList(), + ch_multiqc_custom_config.toList(), + ch_multiqc_logo.toList() + ) + + emit: + multiqc_report = MULTIQC.out.report.toList() // channel: /path/to/multiqc_report.html + versions = ch_versions // channel: [ path(versions.yml) ] +} + +/* +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ + THE END +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ +*/ diff --git a/workflows/nf-cmgg-exomecnv.nf b/workflows/nf-cmgg-exomecnv.nf deleted file mode 100644 index 8cdfbf9..0000000 --- a/workflows/nf-cmgg-exomecnv.nf +++ /dev/null @@ -1,134 +0,0 @@ -/* -~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ - PRINT PARAMS SUMMARY -~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ -*/ - -include { paramsSummaryLog; paramsSummaryMap } from 'plugin/nf-validation' - -def logo = NfcoreTemplate.logo(workflow, params.monochrome_logs) -def citation = '\n' + WorkflowMain.citation(workflow) + '\n' -def summary_params = paramsSummaryMap(workflow) - -// Print parameter summary log to screen -log.info logo + paramsSummaryLog(workflow) + citation - -WorkflowNf-cmgg-exomecnv.initialise(params, log) - -/* -~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ - CONFIG FILES -~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ -*/ - -ch_multiqc_config = Channel.fromPath("$projectDir/assets/multiqc_config.yml", checkIfExists: true) -ch_multiqc_custom_config = params.multiqc_config ? Channel.fromPath( params.multiqc_config, checkIfExists: true ) : Channel.empty() -ch_multiqc_logo = params.multiqc_logo ? Channel.fromPath( params.multiqc_logo, checkIfExists: true ) : Channel.empty() -ch_multiqc_custom_methods_description = params.multiqc_methods_description ? file(params.multiqc_methods_description, checkIfExists: true) : file("$projectDir/assets/methods_description_template.yml", checkIfExists: true) - -/* -~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ - IMPORT LOCAL MODULES/SUBWORKFLOWS -~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ -*/ - -// -// SUBWORKFLOW: Consisting of a mix of local and nf-core/modules -// -include { INPUT_CHECK } from '../subworkflows/local/input_check' - -/* -~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ - IMPORT NF-CORE MODULES/SUBWORKFLOWS -~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ -*/ - -// -// MODULE: Installed directly from nf-core/modules -// -include { FASTQC } from '../modules/nf-core/fastqc/main' -include { MULTIQC } from '../modules/nf-core/multiqc/main' -include { CUSTOM_DUMPSOFTWAREVERSIONS } from '../modules/nf-core/custom/dumpsoftwareversions/main' - -/* -~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ - RUN MAIN WORKFLOW -~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ -*/ - -// Info required for completion email and summary -def multiqc_report = [] - -workflow NF-CMGG-EXOMECNV { - - ch_versions = Channel.empty() - - // - // SUBWORKFLOW: Read in samplesheet, validate and stage input files - // - INPUT_CHECK ( - file(params.input) - ) - ch_versions = ch_versions.mix(INPUT_CHECK.out.versions) - // TODO: OPTIONAL, you can use nf-validation plugin to create an input channel from the samplesheet with Channel.fromSamplesheet("input") - // See the documentation https://nextflow-io.github.io/nf-validation/samplesheets/fromSamplesheet/ - // ! There is currently no tooling to help you write a sample sheet schema - - // - // MODULE: Run FastQC - // - FASTQC ( - INPUT_CHECK.out.reads - ) - ch_versions = ch_versions.mix(FASTQC.out.versions.first()) - - CUSTOM_DUMPSOFTWAREVERSIONS ( - ch_versions.unique().collectFile(name: 'collated_versions.yml') - ) - - // - // MODULE: MultiQC - // - workflow_summary = WorkflowNf-cmgg-exomecnv.paramsSummaryMultiqc(workflow, summary_params) - ch_workflow_summary = Channel.value(workflow_summary) - - methods_description = WorkflowNf-cmgg-exomecnv.methodsDescriptionText(workflow, ch_multiqc_custom_methods_description, params) - ch_methods_description = Channel.value(methods_description) - - ch_multiqc_files = Channel.empty() - ch_multiqc_files = ch_multiqc_files.mix(ch_workflow_summary.collectFile(name: 'workflow_summary_mqc.yaml')) - ch_multiqc_files = ch_multiqc_files.mix(ch_methods_description.collectFile(name: 'methods_description_mqc.yaml')) - ch_multiqc_files = ch_multiqc_files.mix(CUSTOM_DUMPSOFTWAREVERSIONS.out.mqc_yml.collect()) - ch_multiqc_files = ch_multiqc_files.mix(FASTQC.out.zip.collect{it[1]}.ifEmpty([])) - - MULTIQC ( - ch_multiqc_files.collect(), - ch_multiqc_config.toList(), - ch_multiqc_custom_config.toList(), - ch_multiqc_logo.toList() - ) - multiqc_report = MULTIQC.out.report.toList() -} - -/* -~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ - COMPLETION EMAIL AND SUMMARY -~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ -*/ - -workflow.onComplete { - if (params.email || params.email_on_fail) { - NfcoreTemplate.email(workflow, params, summary_params, projectDir, log, multiqc_report) - } - NfcoreTemplate.dump_parameters(workflow, params) - NfcoreTemplate.summary(workflow, params, log) - if (params.hook_url) { - NfcoreTemplate.IM_notification(workflow, params, summary_params, projectDir, log) - } -} - -/* -~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ - THE END -~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ -*/ From 5ce9cfb846c31fffd5107687092187a6c5e3ee42 Mon Sep 17 00:00:00 2001 From: torossee Date: Mon, 18 Mar 2024 11:47:28 +0100 Subject: [PATCH 11/64] add changes --- workflows/exomecnv.nf | 31 +++++++++++++++++++++++-------- 1 file changed, 23 insertions(+), 8 deletions(-) diff --git a/workflows/exomecnv.nf b/workflows/exomecnv.nf index 12ac0fd..434dea1 100644 --- a/workflows/exomecnv.nf +++ b/workflows/exomecnv.nf @@ -4,13 +4,15 @@ ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ */ -include { FASTQC } from '../modules/nf-core/fastqc/main' include { MULTIQC } from '../modules/nf-core/multiqc/main' include { paramsSummaryMap } from 'plugin/nf-validation' include { paramsSummaryMultiqc } from '../subworkflows/nf-core/utils_nfcore_pipeline' include { softwareVersionsToYAML } from '../subworkflows/nf-core/utils_nfcore_pipeline' include { methodsDescriptionText } from '../subworkflows/local/utils_nfcore_exomecnv_pipeline' +// local +include { CRAM_PREPARE } from '../subworkflows/local/cram_prepare/main' +include { BAM_VARIANT_CALLING_EXOMEDEPTH } from '../subworkflows/local/bam_variant_calling_exomedepth/main' /* ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ RUN MAIN WORKFLOW @@ -27,15 +29,28 @@ workflow EXOMECNV { ch_versions = Channel.empty() ch_multiqc_files = Channel.empty() - // - // MODULE: Run FastQC - // - FASTQC ( - ch_samplesheet + // branch into CRAM and BAM files + ch_input.branch { meta, cram, crai -> + CRAM: cram.extension == "cram" + BAM: cram.extension == "bam" + } + .set{ ch_input_prepare } + // ch_input_prepare.BAM.view { "BAM: $it" } + // ch_input_prepare.CRAM.view { "CRAM: $it" } + + // SUBWORKFLOW: Convert CRAM to BAM if no BAM file was provided + ch_fasta = Channel.fromPath(params.fasta).map{ [[id:"reference"], it]}.collect() + ch_fai = params.fai ? Channel.fromPath(params.fai).map{ [[id:"reference"], it]}.collect() : null + + CRAM_PREPARE ( + ch_input_prepare.CRAM, ch_fasta, ch_fai ) - ch_multiqc_files = ch_multiqc_files.mix(FASTQC.out.zip.collect{it[1]}) - ch_versions = ch_versions.mix(FASTQC.out.versions.first()) + + ch_input_prepare.BAM + .mix(CRAM_PREPARE.out.bam) + .set{ ch_input_bam } + ch_input_bam.view() // // Collate and save software versions // From 6301693e9300c63acfe0d7bb3e92f45820620e9c Mon Sep 17 00:00:00 2001 From: torossee Date: Mon, 18 Mar 2024 14:12:41 +0100 Subject: [PATCH 12/64] last fixes new tooling --- main.nf | 39 ++++++++----------- nextflow_schema.json | 1 - .../utils_nfcore_exomecnv_pipeline/main.nf | 16 -------- workflows/exomecnv.nf | 4 +- 4 files changed, 19 insertions(+), 41 deletions(-) diff --git a/main.nf b/main.nf index 8e12b59..fcca6fc 100644 --- a/main.nf +++ b/main.nf @@ -11,28 +11,28 @@ nextflow.enable.dsl = 2 /* ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ - IMPORT FUNCTIONS / MODULES / SUBWORKFLOWS / WORKFLOWS + GENOME PARAMETER VALUES ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ */ - -include { EXOMECNV } from './workflows/exomecnv' -include { PIPELINE_INITIALISATION } from './subworkflows/local/utils_nfcore_exomecnv_pipeline' -include { PIPELINE_COMPLETION } from './subworkflows/local/utils_nfcore_exomecnv_pipeline' - +// use getGenomeAttribute() to fetch parameters +// from igenomes.config using `--genome` include { getGenomeAttribute } from './subworkflows/local/utils_nfcore_exomecnv_pipeline' +params.fasta = getGenomeAttribute('fasta') +params.fai = getGenomeAttribute('fai') + /* ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ - GENOME PARAMETER VALUES + IMPORT FUNCTIONS / MODULES / SUBWORKFLOWS / WORKFLOWS ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ */ -// use getGenomeAttribute() to fetch parameters -// from igenomes.config using `--genome` -params.fasta = WorkflowMain.getGenomeAttribute(params, 'fasta') -params.fai = WorkflowMain.getGenomeAttribute(params, 'fai') +include { EXOMECNV } from './workflows/exomecnv' +include { PIPELINE_INITIALISATION } from './subworkflows/local/utils_nfcore_exomecnv_pipeline' +include { PIPELINE_COMPLETION } from './subworkflows/local/utils_nfcore_exomecnv_pipeline' /* + ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ NAMED WORKFLOWS FOR PIPELINE ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ @@ -43,10 +43,6 @@ params.fai = WorkflowMain.getGenomeAttribute(params, 'fai') // workflow NFCMGG_EXOMECNV { - take: - samplesheet // channel: samplesheet read in from --input - - main: // // SUBWORKFLOW: Run initialisation tasks // @@ -63,7 +59,8 @@ workflow NFCMGG_EXOMECNV { // // WORKFLOW: Run main workflow // - NFCMGG_EXOMECNV ( + + EXOMECNV ( PIPELINE_INITIALISATION.out.samplesheet ) @@ -77,19 +74,17 @@ workflow NFCMGG_EXOMECNV { params.outdir, params.monochrome_logs, params.hook_url, - NFCMGG_EXOMECNV.out.multiqc_report + EXOMECNV.out.multiqc_report ) // // WORKFLOW: Run pipeline // - EXOMECNV ( - samplesheet - ) - emit: - multiqc_report = EXOMECNV.out.multiqc_report // channel: /path/to/multiqc_report.html } +workflow { + NFCMGG_EXOMECNV() +} /* ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ diff --git a/nextflow_schema.json b/nextflow_schema.json index c521b60..7572754 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -19,7 +19,6 @@ "schema": "assets/schema_input.json", "mimetype": "text/csv", "pattern": "^\\S+\\.csv$", - "schema": "assets/schema_input.json", "description": "Path to comma-separated file containing information about the samples in the experiment.", "help_text": "You will need to create a design file with information about the samples in your experiment before running the pipeline. Use this parameter to specify its location. It has to be a comma-separated file with 3 columns, and a header row.", "fa_icon": "fas fa-file-csv" diff --git a/subworkflows/local/utils_nfcore_exomecnv_pipeline/main.nf b/subworkflows/local/utils_nfcore_exomecnv_pipeline/main.nf index 442a301..8c8b7a2 100644 --- a/subworkflows/local/utils_nfcore_exomecnv_pipeline/main.nf +++ b/subworkflows/local/utils_nfcore_exomecnv_pipeline/main.nf @@ -82,22 +82,6 @@ workflow PIPELINE_INITIALISATION { // Channel .fromSamplesheet("input") - .map { - meta, fastq_1, fastq_2 -> - if (!fastq_2) { - return [ meta.id, meta + [ single_end:true ], [ fastq_1 ] ] - } else { - return [ meta.id, meta + [ single_end:false ], [ fastq_1, fastq_2 ] ] - } - } - .groupTuple() - .map { - validateInputSamplesheet(it) - } - .map { - meta, fastqs -> - return [ meta, fastqs.flatten() ] - } .set { ch_samplesheet } emit: diff --git a/workflows/exomecnv.nf b/workflows/exomecnv.nf index 434dea1..956afaf 100644 --- a/workflows/exomecnv.nf +++ b/workflows/exomecnv.nf @@ -28,9 +28,9 @@ workflow EXOMECNV { ch_versions = Channel.empty() ch_multiqc_files = Channel.empty() - + // ch_samplesheet.view() // branch into CRAM and BAM files - ch_input.branch { meta, cram, crai -> + ch_samplesheet.branch { meta, cram, crai -> CRAM: cram.extension == "cram" BAM: cram.extension == "bam" } From 12b20ce4a5501c5ebf2d0d4dc1c55a1e6a6eb4f8 Mon Sep 17 00:00:00 2001 From: torossee Date: Mon, 18 Mar 2024 14:39:42 +0100 Subject: [PATCH 13/64] update nf-core modules --- conf/test.config | 2 +- main.nf | 2 +- modules.json | 20 ++-- .../dumpsoftwareversions/environment.yml | 7 ++ .../custom/dumpsoftwareversions/main.nf | 24 +++++ .../custom/dumpsoftwareversions/meta.yml | 37 +++++++ .../templates/dumpsoftwareversions.py | 101 ++++++++++++++++++ .../dumpsoftwareversions/tests/main.nf.test | 43 ++++++++ .../tests/main.nf.test.snap | 33 ++++++ .../dumpsoftwareversions/tests/tags.yml | 2 + .../nf-core/samtools/convert/environment.yml | 3 +- modules/nf-core/samtools/convert/main.nf | 11 +- modules/nf-core/samtools/convert/meta.yml | 19 +++- .../samtools/convert/tests/main.nf.test | 71 ++++++------ .../samtools/convert/tests/main.nf.test.snap | 64 +++++++++-- subworkflows/local/cram_prepare/main.nf | 2 +- 16 files changed, 368 insertions(+), 73 deletions(-) create mode 100644 modules/nf-core/custom/dumpsoftwareversions/environment.yml create mode 100644 modules/nf-core/custom/dumpsoftwareversions/main.nf create mode 100644 modules/nf-core/custom/dumpsoftwareversions/meta.yml create mode 100755 modules/nf-core/custom/dumpsoftwareversions/templates/dumpsoftwareversions.py create mode 100644 modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test create mode 100644 modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test.snap create mode 100644 modules/nf-core/custom/dumpsoftwareversions/tests/tags.yml diff --git a/conf/test.config b/conf/test.config index a2c12e1..accf1c0 100644 --- a/conf/test.config +++ b/conf/test.config @@ -26,7 +26,7 @@ params { // Genome references fasta = 'https://github.com/nf-cmgg/test-datasets/tree/main/data/genomics/homo_sapiens/genome/seq/GCA_000001405.15_GRCh38_full_plus_hs38d1_analysis_set_chr21.fna' - fai = 'https://github.com/nf-cmgg/test-datasets/tree/main/data/genomics/homo_sapiens/genome/seq/GCA_000001405.15_GRCh38_full_plus_hs38d1_analysis_set_chr21.fna.fai' + // fai = 'https://github.com/nf-cmgg/test-datasets/tree/main/data/genomics/homo_sapiens/genome/seq/GCA_000001405.15_GRCh38_full_plus_hs38d1_analysis_set_chr21.fna.fai' // Parameters diff --git a/main.nf b/main.nf index fcca6fc..1646d5a 100644 --- a/main.nf +++ b/main.nf @@ -19,7 +19,7 @@ nextflow.enable.dsl = 2 include { getGenomeAttribute } from './subworkflows/local/utils_nfcore_exomecnv_pipeline' params.fasta = getGenomeAttribute('fasta') -params.fai = getGenomeAttribute('fai') +// params.fai = getGenomeAttribute('fai') /* ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ diff --git a/modules.json b/modules.json index b14ca42..5933d8c 100644 --- a/modules.json +++ b/modules.json @@ -7,24 +7,18 @@ "nf-core": { "custom/dumpsoftwareversions": { "branch": "master", - "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", - "installed_by": [ - "modules" - ] + "git_sha": "de45447d060b8c8b98575bc637a4a575fd0638e1", + "installed_by": ["modules"] }, "multiqc": { "branch": "master", - "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", - "installed_by": [ - "modules" - ] + "git_sha": "b7ebe95761cd389603f9cc0e0dc384c0f663815a", + "installed_by": ["modules"] }, "samtools/convert": { "branch": "master", - "git_sha": "a64788f5ad388f1d2ac5bd5f1f3f8fc81476148c", - "installed_by": [ - "modules" - ] + "git_sha": "1b93e06140e6807cc408519759f68bd26da63a00", + "installed_by": ["modules"] } } }, @@ -49,4 +43,4 @@ } } } -} \ No newline at end of file +} diff --git a/modules/nf-core/custom/dumpsoftwareversions/environment.yml b/modules/nf-core/custom/dumpsoftwareversions/environment.yml new file mode 100644 index 0000000..b48ced2 --- /dev/null +++ b/modules/nf-core/custom/dumpsoftwareversions/environment.yml @@ -0,0 +1,7 @@ +name: custom_dumpsoftwareversions +channels: + - conda-forge + - bioconda + - defaults +dependencies: + - bioconda::multiqc=1.20 diff --git a/modules/nf-core/custom/dumpsoftwareversions/main.nf b/modules/nf-core/custom/dumpsoftwareversions/main.nf new file mode 100644 index 0000000..105f926 --- /dev/null +++ b/modules/nf-core/custom/dumpsoftwareversions/main.nf @@ -0,0 +1,24 @@ +process CUSTOM_DUMPSOFTWAREVERSIONS { + label 'process_single' + + // Requires `pyyaml` which does not have a dedicated container but is in the MultiQC container + conda "${moduleDir}/environment.yml" + container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? + 'https://depot.galaxyproject.org/singularity/multiqc:1.20--pyhdfd78af_0' : + 'biocontainers/multiqc:1.20--pyhdfd78af_0' }" + + input: + path versions + + output: + path "software_versions.yml" , emit: yml + path "software_versions_mqc.yml", emit: mqc_yml + path "versions.yml" , emit: versions + + when: + task.ext.when == null || task.ext.when + + script: + def args = task.ext.args ?: '' + template 'dumpsoftwareversions.py' +} diff --git a/modules/nf-core/custom/dumpsoftwareversions/meta.yml b/modules/nf-core/custom/dumpsoftwareversions/meta.yml new file mode 100644 index 0000000..5f15a5f --- /dev/null +++ b/modules/nf-core/custom/dumpsoftwareversions/meta.yml @@ -0,0 +1,37 @@ +# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/meta-schema.json +name: custom_dumpsoftwareversions +description: Custom module used to dump software versions within the nf-core pipeline template +keywords: + - custom + - dump + - version +tools: + - custom: + description: Custom module used to dump software versions within the nf-core pipeline template + homepage: https://github.com/nf-core/tools + documentation: https://github.com/nf-core/tools + licence: ["MIT"] +input: + - versions: + type: file + description: YML file containing software versions + pattern: "*.yml" +output: + - yml: + type: file + description: Standard YML file containing software versions + pattern: "software_versions.yml" + - mqc_yml: + type: file + description: MultiQC custom content YML file containing software versions + pattern: "software_versions_mqc.yml" + - versions: + type: file + description: File containing software versions + pattern: "versions.yml" +authors: + - "@drpatelh" + - "@grst" +maintainers: + - "@drpatelh" + - "@grst" diff --git a/modules/nf-core/custom/dumpsoftwareversions/templates/dumpsoftwareversions.py b/modules/nf-core/custom/dumpsoftwareversions/templates/dumpsoftwareversions.py new file mode 100755 index 0000000..da03340 --- /dev/null +++ b/modules/nf-core/custom/dumpsoftwareversions/templates/dumpsoftwareversions.py @@ -0,0 +1,101 @@ +#!/usr/bin/env python + + +"""Provide functions to merge multiple versions.yml files.""" + + +import yaml +import platform +from textwrap import dedent + + +def _make_versions_html(versions): + """Generate a tabular HTML output of all versions for MultiQC.""" + html = [ + dedent( + """\\ + + + + + + + + + + """ + ) + ] + for process, tmp_versions in sorted(versions.items()): + html.append("") + for i, (tool, version) in enumerate(sorted(tmp_versions.items())): + html.append( + dedent( + f"""\\ + + + + + + """ + ) + ) + html.append("") + html.append("
    Process Name Software Version
    {process if (i == 0) else ''}{tool}{version}
    ") + return "\\n".join(html) + + +def main(): + """Load all version files and generate merged output.""" + versions_this_module = {} + versions_this_module["${task.process}"] = { + "python": platform.python_version(), + "yaml": yaml.__version__, + } + + with open("$versions") as f: + versions_by_process = yaml.load(f, Loader=yaml.BaseLoader) | versions_this_module + + # aggregate versions by the module name (derived from fully-qualified process name) + versions_by_module = {} + for process, process_versions in versions_by_process.items(): + module = process.split(":")[-1] + try: + if versions_by_module[module] != process_versions: + raise AssertionError( + "We assume that software versions are the same between all modules. " + "If you see this error-message it means you discovered an edge-case " + "and should open an issue in nf-core/tools. " + ) + except KeyError: + versions_by_module[module] = process_versions + + versions_by_module["Workflow"] = { + "Nextflow": "$workflow.nextflow.version", + "$workflow.manifest.name": "$workflow.manifest.version", + } + + versions_mqc = { + "id": "software_versions", + "section_name": "${workflow.manifest.name} Software Versions", + "section_href": "https://github.com/${workflow.manifest.name}", + "plot_type": "html", + "description": "are collected at run time from the software output.", + "data": _make_versions_html(versions_by_module), + } + + with open("software_versions.yml", "w") as f: + yaml.dump(versions_by_module, f, default_flow_style=False) + with open("software_versions_mqc.yml", "w") as f: + yaml.dump(versions_mqc, f, default_flow_style=False) + + with open("versions.yml", "w") as f: + yaml.dump(versions_this_module, f, default_flow_style=False) + + +if __name__ == "__main__": + main() diff --git a/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test b/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test new file mode 100644 index 0000000..b1e1630 --- /dev/null +++ b/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test @@ -0,0 +1,43 @@ +nextflow_process { + + name "Test Process CUSTOM_DUMPSOFTWAREVERSIONS" + script "../main.nf" + process "CUSTOM_DUMPSOFTWAREVERSIONS" + tag "modules" + tag "modules_nfcore" + tag "custom" + tag "dumpsoftwareversions" + tag "custom/dumpsoftwareversions" + + test("Should run without failures") { + when { + process { + """ + def tool1_version = ''' + TOOL1: + tool1: 0.11.9 + '''.stripIndent() + + def tool2_version = ''' + TOOL2: + tool2: 1.9 + '''.stripIndent() + + input[0] = Channel.of(tool1_version, tool2_version).collectFile() + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot( + process.out.versions, + file(process.out.mqc_yml[0]).readLines()[0..10], + file(process.out.yml[0]).readLines()[0..7] + ).match() + } + ) + } + } +} diff --git a/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test.snap b/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test.snap new file mode 100644 index 0000000..5f59a93 --- /dev/null +++ b/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test.snap @@ -0,0 +1,33 @@ +{ + "Should run without failures": { + "content": [ + [ + "versions.yml:md5,76d454d92244589d32455833f7c1ba6d" + ], + [ + "data: \"\\n\\n \\n \\n \\n \\n \\n \\n \\n\\", + " \\n\\n\\n \\n \\n\\", + " \\ \\n\\n\\n\\n \\n \\", + " \\ \\n \\n\\n\\n\\n\\", + " \\n\\n \\n \\n\\", + " \\ \\n\\n\\n\\n\\n\\n \\n\\", + " \\ \\n \\n\\n\\n\\n\\", + " \\n\\n \\n \\n\\" + ], + [ + "CUSTOM_DUMPSOFTWAREVERSIONS:", + " python: 3.11.7", + " yaml: 5.4.1", + "TOOL1:", + " tool1: 0.11.9", + "TOOL2:", + " tool2: '1.9'", + "Workflow:" + ] + ], + "timestamp": "2024-01-09T23:01:18.710682" + } +} \ No newline at end of file diff --git a/modules/nf-core/custom/dumpsoftwareversions/tests/tags.yml b/modules/nf-core/custom/dumpsoftwareversions/tests/tags.yml new file mode 100644 index 0000000..405aa24 --- /dev/null +++ b/modules/nf-core/custom/dumpsoftwareversions/tests/tags.yml @@ -0,0 +1,2 @@ +custom/dumpsoftwareversions: + - modules/nf-core/custom/dumpsoftwareversions/** diff --git a/modules/nf-core/samtools/convert/environment.yml b/modules/nf-core/samtools/convert/environment.yml index e2589d5..cd4ee8c 100644 --- a/modules/nf-core/samtools/convert/environment.yml +++ b/modules/nf-core/samtools/convert/environment.yml @@ -4,4 +4,5 @@ channels: - bioconda - defaults dependencies: - - bioconda::samtools=1.18 + - bioconda::samtools=1.19.2 + - bioconda::htslib=1.19.1 diff --git a/modules/nf-core/samtools/convert/main.nf b/modules/nf-core/samtools/convert/main.nf index fef51ca..3c539e4 100644 --- a/modules/nf-core/samtools/convert/main.nf +++ b/modules/nf-core/samtools/convert/main.nf @@ -4,8 +4,8 @@ process SAMTOOLS_CONVERT { conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/samtools:1.18--h50ea8bc_1' : - 'biocontainers/samtools:1.18--h50ea8bc_1' }" + 'https://depot.galaxyproject.org/singularity/samtools:1.19.2--h50ea8bc_0' : + 'biocontainers/samtools:1.19.2--h50ea8bc_0' }" input: tuple val(meta), path(input), path(index) @@ -13,8 +13,11 @@ process SAMTOOLS_CONVERT { tuple val(meta3), path(fai) output: - tuple val(meta), path("*.{cram,bam}"), path("*.{crai,bai}") , emit: alignment_index - path "versions.yml" , emit: versions + tuple val(meta), path("*.bam") , emit: bam , optional: true + tuple val(meta), path("*.cram") , emit: cram, optional: true + tuple val(meta), path("*.bai") , emit: bai , optional: true + tuple val(meta), path("*.crai") , emit: crai, optional: true + path "versions.yml" , emit: versions when: task.ext.when == null || task.ext.when diff --git a/modules/nf-core/samtools/convert/meta.yml b/modules/nf-core/samtools/convert/meta.yml index f8a1612..5582897 100644 --- a/modules/nf-core/samtools/convert/meta.yml +++ b/modules/nf-core/samtools/convert/meta.yml @@ -39,10 +39,22 @@ output: description: | Groovy Map containing sample information e.g. [ id:'test', single_end:false ] - - alignment_index: + - bam: type: file - description: filtered/converted BAM/CRAM file + index - pattern: "*{.bam/cram,.bai/crai}" + description: filtered/converted BAM file + pattern: "*{.bam}" + - cram: + type: file + description: filtered/converted CRAM file + pattern: "*{cram}" + - bai: + type: file + description: filtered/converted BAM index + pattern: "*{.bai}" + - crai: + type: file + description: filtered/converted CRAM index + pattern: "*{.crai}" - version: type: file description: File containing software version @@ -53,3 +65,4 @@ authors: maintainers: - "@FriederikeHanssen" - "@maxulysse" + - "@matthdsm" diff --git a/modules/nf-core/samtools/convert/tests/main.nf.test b/modules/nf-core/samtools/convert/tests/main.nf.test index 7087293..d2ab583 100644 --- a/modules/nf-core/samtools/convert/tests/main.nf.test +++ b/modules/nf-core/samtools/convert/tests/main.nf.test @@ -14,19 +14,19 @@ nextflow_process { when { process { """ - input[0] = [ - [ id:'test', single_end:false ], // meta map - file(params.test_data['sarscov2']['illumina']['test_paired_end_sorted_bam'], checkIfExists: true), - file(params.test_data['sarscov2']['illumina']['test_paired_end_sorted_bam_bai'], checkIfExists: true) - ] - input[1] = [ - [id:'fasta'], - file(params.test_data['sarscov2']['genome']['genome_fasta'], checkIfExists: true) - ] - input[2] = [ - [id:'fai'], - file(params.test_data['sarscov2']['genome']['genome_fasta_fai'], checkIfExists: true) - ] + input[0] = Channel.of([ + [id: 'test', single_end: false], // meta map + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/bam/test.paired_end.sorted.bam', checkIfExists: true), + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/bam/test.paired_end.sorted.bam.bai', checkIfExists: true) + ]) + input[1] = Channel.of([ + [ id:'fasta' ], // meta map + file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta', checkIfExists: true) + ]) + input[2] = Channel.of([ + [ id:'fai' ], // meta map + file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta.fai', checkIfExists: true) + ]) """ } } @@ -34,14 +34,11 @@ nextflow_process { then { assertAll( { assert process.success }, - { assert snapshot( - file(process.out.alignment_index[0][1]).name, - file(process.out.alignment_index[0][2]).name, - process.out.versions - ).match() } + { assert snapshot(file(process.out.cram[0][1]).name).match("bam_to_cram_alignment") }, + { assert snapshot(file(process.out.crai[0][1]).name).match("bam_to_cram_index") }, + { assert snapshot(process.out.versions).match("bam_to_cram_versions") } ) } - } test("homo_sapiens - [cram, crai], fasta, fai") { @@ -49,19 +46,19 @@ nextflow_process { when { process { """ - input[0] = [ - [ id:'test', single_end:false ], // meta map - file(params.test_data['homo_sapiens']['illumina']['test_paired_end_recalibrated_sorted_cram'], checkIfExists: true), - file(params.test_data['homo_sapiens']['illumina']['test_paired_end_recalibrated_sorted_cram_crai'], checkIfExists: true) - ] - input[1] = [ - [id:'fasta'], - file(params.test_data['homo_sapiens']['genome']['genome_fasta'], checkIfExists: true) - ] - input[2] = [ - [id:'fai'], - file(params.test_data['homo_sapiens']['genome']['genome_fasta_fai'], checkIfExists: true) - ] + input[0] = Channel.of([ + [id: 'test', single_end: false], // meta map + file(params.modules_testdata_base_path + 'genomics/homo_sapiens/illumina/cram/test.paired_end.sorted.cram', checkIfExists: true), + file(params.modules_testdata_base_path + 'genomics/homo_sapiens/illumina/cram/test.paired_end.sorted.cram.crai', checkIfExists: true) + ]) + input[1] = Channel.of([ + [ id:'fasta' ], // meta map + file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/genome.fasta', checkIfExists: true) + ]) + input[2] = Channel.of([ + [ id:'fai' ], // meta map + file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/genome.fasta.fai', checkIfExists: true) + ]) """ } } @@ -69,14 +66,10 @@ nextflow_process { then { assertAll( { assert process.success }, - { assert snapshot( - file(process.out.alignment_index[0][1]).name, - file(process.out.alignment_index[0][2]).name, - process.out.versions - ).match() } + { assert snapshot(file(process.out.bam[0][1]).name).match("cram_to_bam_alignment") }, + { assert snapshot(file(process.out.bai[0][1]).name).match("cram_to_bam_alignment_index") }, + { assert snapshot(process.out.versions).match("cram_to_bam_versions") } ) } - } - } diff --git a/modules/nf-core/samtools/convert/tests/main.nf.test.snap b/modules/nf-core/samtools/convert/tests/main.nf.test.snap index fe32f14..9d56a4e 100644 --- a/modules/nf-core/samtools/convert/tests/main.nf.test.snap +++ b/modules/nf-core/samtools/convert/tests/main.nf.test.snap @@ -1,22 +1,66 @@ { - "homo_sapiens - [cram, crai], fasta, fai": { + "cram_to_bam_alignment": { + "content": [ + "test.bam" + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-03-06T11:14:51.300147176" + }, + "bam_to_cram_alignment": { + "content": [ + "test.cram" + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-03-06T11:14:36.625470184" + }, + "cram_to_bam_versions": { "content": [ - "test.bam", - "test.bam.bai", [ - "versions.yml:md5,bca738d24a22140d2d5c3ed5060a8896" + "versions.yml:md5,dd0aaf61f39c9ce91c74090f3c7ce1d0" ] ], - "timestamp": "2023-12-04T14:18:33.327409248" + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-03-06T11:14:51.308885706" }, - "sarscov2 - [bam, bai], fasta, fai": { + "bam_to_cram_versions": { "content": [ - "test.cram", - "test.cram.crai", [ - "versions.yml:md5,bca738d24a22140d2d5c3ed5060a8896" + "versions.yml:md5,dd0aaf61f39c9ce91c74090f3c7ce1d0" ] ], - "timestamp": "2023-12-04T14:16:15.221347765" + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-03-06T11:14:36.643960504" + }, + "bam_to_cram_index": { + "content": [ + "test.cram.crai" + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-03-06T11:14:36.640009334" + }, + "cram_to_bam_alignment_index": { + "content": [ + "test.bam.bai" + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-03-06T11:14:51.304477426" } } \ No newline at end of file diff --git a/subworkflows/local/cram_prepare/main.nf b/subworkflows/local/cram_prepare/main.nf index 1a6da4a..38a722f 100644 --- a/subworkflows/local/cram_prepare/main.nf +++ b/subworkflows/local/cram_prepare/main.nf @@ -20,7 +20,7 @@ workflow CRAM_PREPARE { ch_versions = ch_versions.mix(CRAM_TO_BAM.out.versions) emit: - bam = CRAM_TO_BAM.out.alignment_index // channel: [ val(meta), [ bam ], [bai] ] + bam = CRAM_TO_BAM.out.bam // channel: [ val(meta), [ bam ], [bai] ] versions = ch_versions // channel: [ versions.yml ] } From ae6a0c52e7ffe762b5b19f179cad70a0f581080d Mon Sep 17 00:00:00 2001 From: torossee Date: Mon, 18 Mar 2024 14:55:52 +0100 Subject: [PATCH 14/64] update changelog --- CHANGELOG.md | 11 ++++------- 1 file changed, 4 insertions(+), 7 deletions(-) diff --git a/CHANGELOG.md b/CHANGELOG.md index 8b4d42a..a5cac08 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -7,10 +7,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0 Initial release of nf-cmgg/exomecnv, created with the [nf-core](https://nf-co.re/) template. -### `Added` - -### `Fixed` - -### `Dependencies` - -### `Deprecated` +### `Changes` +- initiate step 1: CRAM -> BAM +- new repo name `nf-cmgg/exomecnv` +- Upgraded to nf-core v2.13 template From a98cbb87e8933d615c66315d7f3ee76b5951c4d6 Mon Sep 17 00:00:00 2001 From: torossee Date: Mon, 18 Mar 2024 15:55:48 +0100 Subject: [PATCH 15/64] remove unused dumpsoftwareversions module --- modules.json | 27 +++-- .../dumpsoftwareversions/environment.yml | 7 -- .../custom/dumpsoftwareversions/main.nf | 24 ----- .../custom/dumpsoftwareversions/meta.yml | 37 ------- .../templates/dumpsoftwareversions.py | 101 ------------------ .../dumpsoftwareversions/tests/main.nf.test | 43 -------- .../tests/main.nf.test.snap | 33 ------ .../dumpsoftwareversions/tests/tags.yml | 2 - 8 files changed, 16 insertions(+), 258 deletions(-) delete mode 100644 modules/nf-core/custom/dumpsoftwareversions/environment.yml delete mode 100644 modules/nf-core/custom/dumpsoftwareversions/main.nf delete mode 100644 modules/nf-core/custom/dumpsoftwareversions/meta.yml delete mode 100755 modules/nf-core/custom/dumpsoftwareversions/templates/dumpsoftwareversions.py delete mode 100644 modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test delete mode 100644 modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test.snap delete mode 100644 modules/nf-core/custom/dumpsoftwareversions/tests/tags.yml diff --git a/modules.json b/modules.json index 5933d8c..59147b2 100644 --- a/modules.json +++ b/modules.json @@ -5,20 +5,19 @@ "https://github.com/nf-core/modules.git": { "modules": { "nf-core": { - "custom/dumpsoftwareversions": { - "branch": "master", - "git_sha": "de45447d060b8c8b98575bc637a4a575fd0638e1", - "installed_by": ["modules"] - }, "multiqc": { "branch": "master", "git_sha": "b7ebe95761cd389603f9cc0e0dc384c0f663815a", - "installed_by": ["modules"] + "installed_by": [ + "modules" + ] }, "samtools/convert": { "branch": "master", "git_sha": "1b93e06140e6807cc408519759f68bd26da63a00", - "installed_by": ["modules"] + "installed_by": [ + "modules" + ] } } }, @@ -27,20 +26,26 @@ "utils_nextflow_pipeline": { "branch": "master", "git_sha": "5caf7640a9ef1d18d765d55339be751bb0969dfa", - "installed_by": ["subworkflows"] + "installed_by": [ + "subworkflows" + ] }, "utils_nfcore_pipeline": { "branch": "master", "git_sha": "5caf7640a9ef1d18d765d55339be751bb0969dfa", - "installed_by": ["subworkflows"] + "installed_by": [ + "subworkflows" + ] }, "utils_nfvalidation_plugin": { "branch": "master", "git_sha": "5caf7640a9ef1d18d765d55339be751bb0969dfa", - "installed_by": ["subworkflows"] + "installed_by": [ + "subworkflows" + ] } } } } } -} +} \ No newline at end of file diff --git a/modules/nf-core/custom/dumpsoftwareversions/environment.yml b/modules/nf-core/custom/dumpsoftwareversions/environment.yml deleted file mode 100644 index b48ced2..0000000 --- a/modules/nf-core/custom/dumpsoftwareversions/environment.yml +++ /dev/null @@ -1,7 +0,0 @@ -name: custom_dumpsoftwareversions -channels: - - conda-forge - - bioconda - - defaults -dependencies: - - bioconda::multiqc=1.20 diff --git a/modules/nf-core/custom/dumpsoftwareversions/main.nf b/modules/nf-core/custom/dumpsoftwareversions/main.nf deleted file mode 100644 index 105f926..0000000 --- a/modules/nf-core/custom/dumpsoftwareversions/main.nf +++ /dev/null @@ -1,24 +0,0 @@ -process CUSTOM_DUMPSOFTWAREVERSIONS { - label 'process_single' - - // Requires `pyyaml` which does not have a dedicated container but is in the MultiQC container - conda "${moduleDir}/environment.yml" - container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/multiqc:1.20--pyhdfd78af_0' : - 'biocontainers/multiqc:1.20--pyhdfd78af_0' }" - - input: - path versions - - output: - path "software_versions.yml" , emit: yml - path "software_versions_mqc.yml", emit: mqc_yml - path "versions.yml" , emit: versions - - when: - task.ext.when == null || task.ext.when - - script: - def args = task.ext.args ?: '' - template 'dumpsoftwareversions.py' -} diff --git a/modules/nf-core/custom/dumpsoftwareversions/meta.yml b/modules/nf-core/custom/dumpsoftwareversions/meta.yml deleted file mode 100644 index 5f15a5f..0000000 --- a/modules/nf-core/custom/dumpsoftwareversions/meta.yml +++ /dev/null @@ -1,37 +0,0 @@ -# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/meta-schema.json -name: custom_dumpsoftwareversions -description: Custom module used to dump software versions within the nf-core pipeline template -keywords: - - custom - - dump - - version -tools: - - custom: - description: Custom module used to dump software versions within the nf-core pipeline template - homepage: https://github.com/nf-core/tools - documentation: https://github.com/nf-core/tools - licence: ["MIT"] -input: - - versions: - type: file - description: YML file containing software versions - pattern: "*.yml" -output: - - yml: - type: file - description: Standard YML file containing software versions - pattern: "software_versions.yml" - - mqc_yml: - type: file - description: MultiQC custom content YML file containing software versions - pattern: "software_versions_mqc.yml" - - versions: - type: file - description: File containing software versions - pattern: "versions.yml" -authors: - - "@drpatelh" - - "@grst" -maintainers: - - "@drpatelh" - - "@grst" diff --git a/modules/nf-core/custom/dumpsoftwareversions/templates/dumpsoftwareversions.py b/modules/nf-core/custom/dumpsoftwareversions/templates/dumpsoftwareversions.py deleted file mode 100755 index da03340..0000000 --- a/modules/nf-core/custom/dumpsoftwareversions/templates/dumpsoftwareversions.py +++ /dev/null @@ -1,101 +0,0 @@ -#!/usr/bin/env python - - -"""Provide functions to merge multiple versions.yml files.""" - - -import yaml -import platform -from textwrap import dedent - - -def _make_versions_html(versions): - """Generate a tabular HTML output of all versions for MultiQC.""" - html = [ - dedent( - """\\ - -
    Process Name \\", + " \\ Software Version
    CUSTOM_DUMPSOFTWAREVERSIONSpython3.11.7
    yaml5.4.1
    TOOL1tool10.11.9
    TOOL2tool21.9
    WorkflowNextflow
    - - - - - - - - """ - ) - ] - for process, tmp_versions in sorted(versions.items()): - html.append("") - for i, (tool, version) in enumerate(sorted(tmp_versions.items())): - html.append( - dedent( - f"""\\ - - - - - - """ - ) - ) - html.append("") - html.append("
    Process Name Software Version
    {process if (i == 0) else ''}{tool}{version}
    ") - return "\\n".join(html) - - -def main(): - """Load all version files and generate merged output.""" - versions_this_module = {} - versions_this_module["${task.process}"] = { - "python": platform.python_version(), - "yaml": yaml.__version__, - } - - with open("$versions") as f: - versions_by_process = yaml.load(f, Loader=yaml.BaseLoader) | versions_this_module - - # aggregate versions by the module name (derived from fully-qualified process name) - versions_by_module = {} - for process, process_versions in versions_by_process.items(): - module = process.split(":")[-1] - try: - if versions_by_module[module] != process_versions: - raise AssertionError( - "We assume that software versions are the same between all modules. " - "If you see this error-message it means you discovered an edge-case " - "and should open an issue in nf-core/tools. " - ) - except KeyError: - versions_by_module[module] = process_versions - - versions_by_module["Workflow"] = { - "Nextflow": "$workflow.nextflow.version", - "$workflow.manifest.name": "$workflow.manifest.version", - } - - versions_mqc = { - "id": "software_versions", - "section_name": "${workflow.manifest.name} Software Versions", - "section_href": "https://github.com/${workflow.manifest.name}", - "plot_type": "html", - "description": "are collected at run time from the software output.", - "data": _make_versions_html(versions_by_module), - } - - with open("software_versions.yml", "w") as f: - yaml.dump(versions_by_module, f, default_flow_style=False) - with open("software_versions_mqc.yml", "w") as f: - yaml.dump(versions_mqc, f, default_flow_style=False) - - with open("versions.yml", "w") as f: - yaml.dump(versions_this_module, f, default_flow_style=False) - - -if __name__ == "__main__": - main() diff --git a/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test b/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test deleted file mode 100644 index b1e1630..0000000 --- a/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test +++ /dev/null @@ -1,43 +0,0 @@ -nextflow_process { - - name "Test Process CUSTOM_DUMPSOFTWAREVERSIONS" - script "../main.nf" - process "CUSTOM_DUMPSOFTWAREVERSIONS" - tag "modules" - tag "modules_nfcore" - tag "custom" - tag "dumpsoftwareversions" - tag "custom/dumpsoftwareversions" - - test("Should run without failures") { - when { - process { - """ - def tool1_version = ''' - TOOL1: - tool1: 0.11.9 - '''.stripIndent() - - def tool2_version = ''' - TOOL2: - tool2: 1.9 - '''.stripIndent() - - input[0] = Channel.of(tool1_version, tool2_version).collectFile() - """ - } - } - - then { - assertAll( - { assert process.success }, - { assert snapshot( - process.out.versions, - file(process.out.mqc_yml[0]).readLines()[0..10], - file(process.out.yml[0]).readLines()[0..7] - ).match() - } - ) - } - } -} diff --git a/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test.snap b/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test.snap deleted file mode 100644 index 5f59a93..0000000 --- a/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test.snap +++ /dev/null @@ -1,33 +0,0 @@ -{ - "Should run without failures": { - "content": [ - [ - "versions.yml:md5,76d454d92244589d32455833f7c1ba6d" - ], - [ - "data: \"\\n\\n \\n \\n \\n \\n \\n \\n \\n\\", - " \\n\\n\\n \\n \\n\\", - " \\ \\n\\n\\n\\n \\n \\", - " \\ \\n \\n\\n\\n\\n\\", - " \\n\\n \\n \\n\\", - " \\ \\n\\n\\n\\n\\n\\n \\n\\", - " \\ \\n \\n\\n\\n\\n\\", - " \\n\\n \\n \\n\\" - ], - [ - "CUSTOM_DUMPSOFTWAREVERSIONS:", - " python: 3.11.7", - " yaml: 5.4.1", - "TOOL1:", - " tool1: 0.11.9", - "TOOL2:", - " tool2: '1.9'", - "Workflow:" - ] - ], - "timestamp": "2024-01-09T23:01:18.710682" - } -} \ No newline at end of file diff --git a/modules/nf-core/custom/dumpsoftwareversions/tests/tags.yml b/modules/nf-core/custom/dumpsoftwareversions/tests/tags.yml deleted file mode 100644 index 405aa24..0000000 --- a/modules/nf-core/custom/dumpsoftwareversions/tests/tags.yml +++ /dev/null @@ -1,2 +0,0 @@ -custom/dumpsoftwareversions: - - modules/nf-core/custom/dumpsoftwareversions/** From 90000d73d32a85c48577c0708b5c12accecad215 Mon Sep 17 00:00:00 2001 From: torossee Date: Tue, 19 Mar 2024 09:50:37 +0100 Subject: [PATCH 16/64] fix .fai index --- conf/test.config | 2 +- main.nf | 2 +- nextflow_schema.json | 9 +++++++++ 3 files changed, 11 insertions(+), 2 deletions(-) diff --git a/conf/test.config b/conf/test.config index accf1c0..a2c12e1 100644 --- a/conf/test.config +++ b/conf/test.config @@ -26,7 +26,7 @@ params { // Genome references fasta = 'https://github.com/nf-cmgg/test-datasets/tree/main/data/genomics/homo_sapiens/genome/seq/GCA_000001405.15_GRCh38_full_plus_hs38d1_analysis_set_chr21.fna' - // fai = 'https://github.com/nf-cmgg/test-datasets/tree/main/data/genomics/homo_sapiens/genome/seq/GCA_000001405.15_GRCh38_full_plus_hs38d1_analysis_set_chr21.fna.fai' + fai = 'https://github.com/nf-cmgg/test-datasets/tree/main/data/genomics/homo_sapiens/genome/seq/GCA_000001405.15_GRCh38_full_plus_hs38d1_analysis_set_chr21.fna.fai' // Parameters diff --git a/main.nf b/main.nf index 1646d5a..fcca6fc 100644 --- a/main.nf +++ b/main.nf @@ -19,7 +19,7 @@ nextflow.enable.dsl = 2 include { getGenomeAttribute } from './subworkflows/local/utils_nfcore_exomecnv_pipeline' params.fasta = getGenomeAttribute('fasta') -// params.fai = getGenomeAttribute('fai') +params.fai = getGenomeAttribute('fai') /* ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ diff --git a/nextflow_schema.json b/nextflow_schema.json index 7572754..3c18ee8 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -65,6 +65,15 @@ "help_text": "This parameter is *mandatory* if `--genome` is not specified. If you don't have a BWA index available this will be generated for you automatically. Combine with `--save_reference` to save BWA index for future runs.", "fa_icon": "far fa-file-code" }, + "fai": { + "type": "string", + "format": "file-path", + "exists": true, + "mimetype": "text/plain", + "pattern": "^\\S+\\.fai$", + "description": "Path to FASTA genome index file.", + "fa_icon": "far fa-file-code" + }, "igenomes_ignore": { "type": "boolean", "description": "Do not load the iGenomes reference config.", From eba5f5ba9862c6b7852c987214354b8ccd90b876 Mon Sep 17 00:00:00 2001 From: torossee Date: Wed, 20 Mar 2024 10:57:41 +0100 Subject: [PATCH 17/64] finish code exomedepth count per sample --- ...omeDepth_counting.R => ExomeDepth_count.R} | 77 +++++++------------ conf/test.config | 2 + main.nf | 4 +- .../local/exomedepth/count/environment.yml | 10 +++ modules/local/exomedepth/count/main.nf | 27 ++++--- nextflow.config | 7 +- nextflow_schema.json | 37 ++++++++- subworkflows/local/cram_prepare/main.nf | 3 +- subworkflows/local/exomedepth_count/main.nf | 26 +++++++ .../local/exomedepth_counting/main.nf | 25 ------ .../utils_nfcore_exomecnv_pipeline/main.nf | 2 + workflows/exomecnv.nf | 30 ++++++-- 12 files changed, 153 insertions(+), 97 deletions(-) rename bin/{CNV_ExomeDepth_counting.R => ExomeDepth_count.R} (56%) mode change 100644 => 100755 create mode 100644 modules/local/exomedepth/count/environment.yml create mode 100644 subworkflows/local/exomedepth_count/main.nf delete mode 100644 subworkflows/local/exomedepth_counting/main.nf diff --git a/bin/CNV_ExomeDepth_counting.R b/bin/ExomeDepth_count.R old mode 100644 new mode 100755 similarity index 56% rename from bin/CNV_ExomeDepth_counting.R rename to bin/ExomeDepth_count.R index 6412008..8cf5d0a --- a/bin/CNV_ExomeDepth_counting.R +++ b/bin/ExomeDepth_count.R @@ -1,45 +1,35 @@ +#!/usr/bin/env Rscript + ############################################################################################ ### ExomeDepth - counting step ### ############################################################################################ ### load libs ### -library("optparse") +# library("optparse") library("methods") # to load the function "as" library("ExomeDepth") ExomeDepthVersion <- packageDescription("ExomeDepth")$Version print(paste0("...loaded ExomeDepth version: ", ExomeDepthVersion)) -## pass options ### -option_list = list( - make_option(c("--bamfile"), type="character", default=NULL, - help="full paths of BAM file", metavar="character"), - make_option(c("--exon_target"), type="character", default=NULL, - help="path to bed file of exon target", metavar="character"), - make_option(c("--prefix"), type="character", default=NULL, - help="prefix for output name, chrX or autosomal", metavar="character") -); -opt_parser = OptionParser(option_list=option_list); -opt = parse_args(opt_parser); -if (is.null(opt$bamfile)){ - print_help(opt_parser) - stop("Not all the input arguments are provided", call.=FALSE) -} -if (is.null(opt$exon_target)){ - print_help(opt_parser) - stop("Not all the input arguments are provided", call.=FALSE) +## pass options ### +args = commandArgs(trailingOnly = TRUE) +print_usage <- function() { + cat("Usage: ExomeDepth_count.R \n") } -if (is.null(opt$prefix)){ - print_help(opt_parser) - stop("Not all the input arguments are provided", call.=FALSE) +if (length(commandArgs(trailingOnly = TRUE)) != 5) { + print_usage() + q("no", status = 1) # Terminate the script with an error status } -cat("bam file: ", opt$bamfile, "\n") -cat("exon target file: ", opt$exon_target, "\n") -cat("prefix: ", opt$prefix, "\n") +cat("samplename: ", args[1], "\n") +cat("bam file: ", args[2], "\n") +cat("bam index file: ", args[3], "\n") +cat("exon target file: ", args[4], "\n") +cat("prefix: ", args[5], "\n") ### load exon data ### -exons <- read.table(file=opt$exon_target,sep="\t",header = F) +exons <- read.table(file=args[4],sep="\t",header = F) colnames(exons) <- c("chromosome","start","end","name") ### load all exon info #### @@ -49,30 +39,14 @@ exons.GRanges <- GenomicRanges::GRanges(seqnames = exons$chromosome, ) ### Getting the input data from BAM file ### -bam <- unlist(opt$bamfile ) - -sampleName <- unlist(strsplit(unlist(strsplit(bam, ".bam")),split=".//")) +bam <- unlist(args[2]) cat("Sample names:", "\n") -sampleName2<-gsub("^.*/","",sampleName) -sampleName2 +sampleName <- unlist(args[1]) +sampleName ### ExomeDepth sometimes spectacularly fails to find the BAM index, for no clear reason ### ## so help it out by testing a few likely filenames -bamindex = bam - -stardotbai = gsub(".bam$",".bai",bam) -stardotbamdotbai = gsub(".bam$",".bam.bai",bam) -if (file.exists(stardotbai)) { - bamindexe = stardotbai -} else if (file.exists(stardotbamdotbai)) { - bamindexe = stardotbamdotbai -} -else { - cat(paste("Cannot find a .bai index for BAM: ",bam,"\n",sep=""),file=stderr()) - cat("stopping execution....",file=stderr()) - stop() -} ### read.count ~exomeCopy package ### ## ouput = GenomicRages object that stores the read count data form the BAM file ### @@ -84,22 +58,27 @@ ExomeCount <- getBamCounts(bed.frame = exons, #min.mapq =20 #default minimum mapping quality to include a read #read.width =300 #default maximum distance between the side of the target region and the middle of the paired reads to include the paired read into that region ) +cat("\nCounting done...\n") +colnames(ExomeCount) ## convert GRanges class (S4 object) into a data.frame, which is the input format for ExomeDepth ExomeCount.dafr <- as(ExomeCount[, colnames(ExomeCount)], 'data.frame') +cat("\nConversion done...\n") +print(head(ExomeCount.dafr)) ## remove the annoying chr letters -ExomeCount.dafr$space <- gsub(as.character(ExomeCount.dafr$space), +ExomeCount.dafr$chromosome <- gsub(as.character(ExomeCount.dafr$chromosome), pattern = 'chr', replacement = '') +cat("\nChr's removed...\n") ## rename sample names in colnames -header_first_part<-c("space", "start", "end", "width", "names") -colnames(ExomeCount.dafr)<-c(header_first_part, sampleName2) +header_first_part<-c("chromosome", "start", "end", "exon") +colnames(ExomeCount.dafr)<-c(header_first_part, sampleName) colnames(ExomeCount.dafr) print(head(ExomeCount.dafr)) cat("Successfully calculated counts.\n") ### save counts as a text file and rda object ### cat("Saving the counts \n") -countspath = paste("counts_",sampleName2,"_",opt$prefix,".txt",sep="") +countspath = paste("counts_",sampleName,"_",args[5],".txt",sep="") write.table(ExomeCount.dafr,countspath,sep="\t",col.names=TRUE,row.names=FALSE,quote=FALSE) cat("\n\n---Finished---\n") diff --git a/conf/test.config b/conf/test.config index a2c12e1..e76a22d 100644 --- a/conf/test.config +++ b/conf/test.config @@ -29,5 +29,7 @@ params { fai = 'https://github.com/nf-cmgg/test-datasets/tree/main/data/genomics/homo_sapiens/genome/seq/GCA_000001405.15_GRCh38_full_plus_hs38d1_analysis_set_chr21.fna.fai' // Parameters + roi_auto = "/home/torossee/projects/nf-cmgg-exomecnv_references/Homo_sapiens.GRCh38.105.chr21_protein_coding_basic_sorted_merged_autosomal.bed" + roi_chrx = "/home/torossee/projects/nf-cmgg-exomecnv_references/Homo_sapiens.GRCh38.105.chrX_protein_coding_basic_sorted_merged.bed" } diff --git a/main.nf b/main.nf index fcca6fc..01983aa 100644 --- a/main.nf +++ b/main.nf @@ -53,7 +53,9 @@ workflow NFCMGG_EXOMECNV { params.monochrome_logs, args, params.outdir, - params.input + params.input, + params.roi_auto, + params.roi_chrx ) // diff --git a/modules/local/exomedepth/count/environment.yml b/modules/local/exomedepth/count/environment.yml new file mode 100644 index 0000000..4cb9d93 --- /dev/null +++ b/modules/local/exomedepth/count/environment.yml @@ -0,0 +1,10 @@ +name: exomedepth_count +channels: + - conda-forge + - bioconda + - defaults + +dependencies: + - r=3.6=r36_1003 + - r-optparse=1.6.4=r36h6115d3f_0 + - r-exomedepth=1.1.12=r36h6786f55_0 diff --git a/modules/local/exomedepth/count/main.nf b/modules/local/exomedepth/count/main.nf index 2cd3d6d..b48ad0e 100644 --- a/modules/local/exomedepth/count/main.nf +++ b/modules/local/exomedepth/count/main.nf @@ -1,29 +1,36 @@ -process EXOMEDEPTH_COUNT { - tag "$meta.id $prefix" +process COUNT { + tag "$meta.id $prefix.id" label 'process_low' - conda "conda-forge::r=3.6" + conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/r-exomedepth:1.1.12--r36h6786f55_0' : - 'biocontainers/r-exomedepth:1.1.12--r36h6786f55_0' }" + 'https://depot.galaxyproject.org/singularity/r-exomedepth:1.1.16--r43hfb3cda0_3' : + 'biocontainers/r-exomedepth:1.1.16--r43hfb3cda0_3' }" + + publishDir "$params.outdir/exomedepth/counts", mode: 'copy' input: tuple val(meta), path(bam), path(bai) tuple val(prefix), path(exon_target) output: - path '*.txt' , emit: counts + tuple val(meta), path("*.txt"), emit: counts path "versions.yml", emit: versions when: task.ext.when == null || task.ext.when script: // This script is bundled with the pipeline, in nf-cmgg/exomecnv/bin/ - - def VERSION = '1.1.12' - + + def VERSION = '1.1.16' + """ - Rscript CNV_ExomeDepth_counting.R + ExomeDepth_count.R \\ + $meta.id \\ + $bam \\ + $bai \\ + $exon_target \\ + $prefix.id cat <<-END_VERSIONS > versions.yml "${task.process}": diff --git a/nextflow.config b/nextflow.config index ea05343..d985c8e 100644 --- a/nextflow.config +++ b/nextflow.config @@ -59,6 +59,11 @@ params { validationShowHiddenParams = false validate_params = true + // ExomeDepth Parameters + roi_auto = "/home/torossee/projects/nf-cmgg-exomecnv_references/Homo_sapiens.GRCh38.105.chr_protein_coding_basic_sorted_merged_autosomal.bed" + roi_chrx = "/home/torossee/projects/nf-cmgg-exomecnv_references/Homo_sapiens.GRCh38.105.chrX_protein_coding_basic_sorted_merged.bed" + + } // Load base.config by default for all pipelines @@ -230,7 +235,7 @@ dag { manifest { name = 'nf-cmgg/exomecnv' - author = """nvnieuwk""" + author = """ToonRosseel""" homePage = 'https://github.com/nf-cmgg/exomecnv' description = """A nextflow pipeline for calling exome CNVs""" mainScript = 'main.nf' diff --git a/nextflow_schema.json b/nextflow_schema.json index 3c18ee8..ec2b6eb 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -53,7 +53,8 @@ "type": "string", "description": "Name of iGenomes reference.", "fa_icon": "fas fa-book", - "help_text": "If using a reference genome configured in the pipeline using iGenomes, use this parameter to give the ID for the reference. This is then used to build the full paths for all required reference genome files e.g. `--genome GRCh38`. \n\nSee the [nf-core website docs](https://nf-co.re/usage/reference_genomes) for more details." + "help_text": "If using a reference genome configured in the pipeline using iGenomes, use this parameter to give the ID for the reference. This is then used to build the full paths for all required reference genome files e.g. `--genome GRCh38`. \n\nSee the [nf-core website docs](https://nf-co.re/usage/reference_genomes) for more details.", + "default": "GRCh38" }, "fasta": { "type": "string", @@ -79,7 +80,8 @@ "description": "Do not load the iGenomes reference config.", "fa_icon": "fas fa-ban", "hidden": true, - "help_text": "Do not load `igenomes.config` when running the pipeline. You may choose this option if you observe clashes between custom parameters and those supplied in `igenomes.config`." + "help_text": "Do not load `igenomes.config` when running the pipeline. You may choose this option if you observe clashes between custom parameters and those supplied in `igenomes.config`.", + "default": true }, "cmgg_config_base": { "type": "string", @@ -87,7 +89,8 @@ "description": "The config base path for the cmgg configs", "format": "directory-path" } - } + }, + "required": ["fasta", "fai"] }, "institutional_config_options": { "title": "Institutional config options", @@ -282,6 +285,31 @@ "help_text": "Allows string values that are parseable as numbers or booleans. For further information see [JSONSchema docs](https://github.com/everit-org/json-schema#lenient-mode)." } } + }, + "exomedepth_parameters": { + "title": "ExomeDepth parameters", + "type": "object", + "description": "Options specific for the ExomeDepth execution", + "default": "", + "properties": { + "roi_auto": { + "type": "string", + "description": "Path to the default ROI (regions of interest) BED file of autosomal (merged) regions to be used for CNV analysis", + "format": "file-path", + "pattern": "^\\S+\\.bed$", + "mimetype": "text/plain", + "default": "/home/torossee/projects/nf-cmgg-exomecnv_references/Homo_sapiens.GRCh38.105.chr_protein_coding_basic_sorted_merged_autosomal.bed" + }, + "roi_chrx": { + "type": "string", + "description": "Path to the default ROI (regions of interest) BED file of chrX (merged) regions to be used for CNV analysis", + "format": "file-path", + "pattern": "^\\S+\\.bed$", + "mimetype": "text/plain", + "default": "/home/torossee/projects/nf-cmgg-exomecnv_references/Homo_sapiens.GRCh38.105.chrX_protein_coding_basic_sorted_merged.bed" + } + }, + "required": ["roi_auto", "roi_chrx"] } }, "allOf": [ @@ -299,6 +327,9 @@ }, { "$ref": "#/definitions/generic_options" + }, + { + "$ref": "#/definitions/exomedepth_parameters" } ] } diff --git a/subworkflows/local/cram_prepare/main.nf b/subworkflows/local/cram_prepare/main.nf index 38a722f..275e672 100644 --- a/subworkflows/local/cram_prepare/main.nf +++ b/subworkflows/local/cram_prepare/main.nf @@ -20,7 +20,8 @@ workflow CRAM_PREPARE { ch_versions = ch_versions.mix(CRAM_TO_BAM.out.versions) emit: - bam = CRAM_TO_BAM.out.bam // channel: [ val(meta), [ bam ], [bai] ] + bam = CRAM_TO_BAM.out.bam // channel: [ val(meta), [ bam ] ] + bai = CRAM_TO_BAM.out.bai // channel: [ val(meta), [ bai ] ] versions = ch_versions // channel: [ versions.yml ] } diff --git a/subworkflows/local/exomedepth_count/main.nf b/subworkflows/local/exomedepth_count/main.nf new file mode 100644 index 0000000..9466417 --- /dev/null +++ b/subworkflows/local/exomedepth_count/main.nf @@ -0,0 +1,26 @@ + +// Convert CRAM files +include { COUNT } from '../../../modules/local/exomedepth/count/main' + +workflow EXOMEDEPTH_COUNT { + + take: + ch_bam // channel: [mandatory] [ val(meta), path(bam), path(bai) ] + exon_target // channel: [mandatory] [ val(prefix), path(bed) ] + + main: + + ch_versions = Channel.empty() + + // + // convert CRAM files to BAM files + // + COUNT(ch_bam, exon_target) + ch_versions = ch_versions.mix(COUNT.out.versions) + + emit: + count = COUNT.out.counts // channel: [ val(meta), [ txt ] ] + + versions = ch_versions // channel: [ versions.yml ] +} + diff --git a/subworkflows/local/exomedepth_counting/main.nf b/subworkflows/local/exomedepth_counting/main.nf deleted file mode 100644 index 3506067..0000000 --- a/subworkflows/local/exomedepth_counting/main.nf +++ /dev/null @@ -1,25 +0,0 @@ - -// Convert CRAM files - -workflow EXOMEDEPTH_COUNTING { - - take: - ch_bam // channel: [mandatory] [ val(meta), path(bam), path(bai) ] => sample BAM files and their indices - exon_target // channel: [mandatory] [ val(meta), path(bed) ] - - main: - - ch_versions = Channel.empty() - - // - // convert CRAM files to BAM files - // - CRAM_TO_BAM(ch_cram, fasta, fasta_fai) - ch_versions = ch_versions.mix(CRAM_TO_BAM.out.versions) - - emit: - bam = CRAM_TO_BAM.out.alignment_index // channel: [ val(meta), [ bam ], [bai] ] - - versions = ch_versions // channel: [ versions.yml ] -} - diff --git a/subworkflows/local/utils_nfcore_exomecnv_pipeline/main.nf b/subworkflows/local/utils_nfcore_exomecnv_pipeline/main.nf index 8c8b7a2..4fc5c8e 100644 --- a/subworkflows/local/utils_nfcore_exomecnv_pipeline/main.nf +++ b/subworkflows/local/utils_nfcore_exomecnv_pipeline/main.nf @@ -36,6 +36,8 @@ workflow PIPELINE_INITIALISATION { nextflow_cli_args // array: List of positional nextflow CLI args outdir // string: The output directory where the results will be saved input // string: Path to input samplesheet + roi_auto // string: Path to ExomeDepth BED file of (merged) autosomal regions + roi_chrx // string: Path to ExomeDepth BED file of (merged) chrX regions main: diff --git a/workflows/exomecnv.nf b/workflows/exomecnv.nf index 956afaf..ed86ca6 100644 --- a/workflows/exomecnv.nf +++ b/workflows/exomecnv.nf @@ -10,9 +10,10 @@ include { paramsSummaryMultiqc } from '../subworkflows/nf-core/utils_nfcore_pi include { softwareVersionsToYAML } from '../subworkflows/nf-core/utils_nfcore_pipeline' include { methodsDescriptionText } from '../subworkflows/local/utils_nfcore_exomecnv_pipeline' -// local +// local include { CRAM_PREPARE } from '../subworkflows/local/cram_prepare/main' -include { BAM_VARIANT_CALLING_EXOMEDEPTH } from '../subworkflows/local/bam_variant_calling_exomedepth/main' +include { EXOMEDEPTH_COUNT } from '../subworkflows/local/exomedepth_count/main' +// include { BAM_VARIANT_CALLING_EXOMEDEPTH } from '../subworkflows/local/bam_variant_calling_exomedepth/main' /* ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ RUN MAIN WORKFLOW @@ -28,7 +29,11 @@ workflow EXOMECNV { ch_versions = Channel.empty() ch_multiqc_files = Channel.empty() - // ch_samplesheet.view() + + // + // Importing and convert the input files passed through the parameters to channels + // + // branch into CRAM and BAM files ch_samplesheet.branch { meta, cram, crai -> CRAM: cram.extension == "cram" @@ -38,19 +43,30 @@ workflow EXOMECNV { // ch_input_prepare.BAM.view { "BAM: $it" } // ch_input_prepare.CRAM.view { "CRAM: $it" } - // SUBWORKFLOW: Convert CRAM to BAM if no BAM file was provided + ch_fasta = Channel.fromPath(params.fasta).map{ [[id:"reference"], it]}.collect() ch_fai = params.fai ? Channel.fromPath(params.fai).map{ [[id:"reference"], it]}.collect() : null + ch_roi_auto = Channel.fromPath(params.roi_auto).map{ [[id:"autosomal"], it]}.collect() + ch_roi_x = Channel.fromPath(params.roi_chrx).map{ [[id:"chrX"], it]}.collect() + + // ch_roi_auto.view() + // ch_roi_auto.view() + // SUBWORKFLOW: Convert CRAM to BAM if no BAM file was provided CRAM_PREPARE ( ch_input_prepare.CRAM, ch_fasta, ch_fai ) - + ch_input_prepare.BAM .mix(CRAM_PREPARE.out.bam) - .set{ ch_input_bam } + .join(CRAM_PREPARE.out.bai) + .set{ ch_input_bam } + + // ch_input_bam.view() - ch_input_bam.view() + EXOMEDEPTH_COUNT ( + ch_input_bam, ch_roi_auto + ) // // Collate and save software versions // From ebef06dc71ae07c87a08f295f64a46a50201e77e Mon Sep 17 00:00:00 2001 From: Toros <89534142+ToonRosseel@users.noreply.github.com> Date: Wed, 20 Mar 2024 11:34:06 +0100 Subject: [PATCH 18/64] Update main.nf Co-authored-by: Nicolas Vannieuwkerke <101190534+nvnieuwk@users.noreply.github.com> --- main.nf | 2 -- 1 file changed, 2 deletions(-) diff --git a/main.nf b/main.nf index 01983aa..472c14e 100644 --- a/main.nf +++ b/main.nf @@ -54,8 +54,6 @@ workflow NFCMGG_EXOMECNV { args, params.outdir, params.input, - params.roi_auto, - params.roi_chrx ) // From 9b93d6fc256f8a212194f569fe50cc125a6e0b5f Mon Sep 17 00:00:00 2001 From: Toros <89534142+ToonRosseel@users.noreply.github.com> Date: Wed, 20 Mar 2024 11:34:27 +0100 Subject: [PATCH 19/64] Update modules/local/exomedepth/count/main.nf Co-authored-by: Nicolas Vannieuwkerke <101190534+nvnieuwk@users.noreply.github.com> --- modules/local/exomedepth/count/main.nf | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/modules/local/exomedepth/count/main.nf b/modules/local/exomedepth/count/main.nf index b48ad0e..21afd3d 100644 --- a/modules/local/exomedepth/count/main.nf +++ b/modules/local/exomedepth/count/main.nf @@ -11,7 +11,7 @@ process COUNT { input: tuple val(meta), path(bam), path(bai) - tuple val(prefix), path(exon_target) + tuple val(meta2), path(exon_target) output: tuple val(meta), path("*.txt"), emit: counts From 0aa92a22638b54a78ed37c1fc913b4d300c735f9 Mon Sep 17 00:00:00 2001 From: Toros <89534142+ToonRosseel@users.noreply.github.com> Date: Wed, 20 Mar 2024 11:34:35 +0100 Subject: [PATCH 20/64] Update modules/local/exomedepth/count/main.nf Co-authored-by: Nicolas Vannieuwkerke <101190534+nvnieuwk@users.noreply.github.com> --- modules/local/exomedepth/count/main.nf | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/modules/local/exomedepth/count/main.nf b/modules/local/exomedepth/count/main.nf index 21afd3d..b0d96fe 100644 --- a/modules/local/exomedepth/count/main.nf +++ b/modules/local/exomedepth/count/main.nf @@ -30,7 +30,7 @@ process COUNT { $bam \\ $bai \\ $exon_target \\ - $prefix.id + $meta2.id cat <<-END_VERSIONS > versions.yml "${task.process}": From 8152e7b414f09a1fdcfedd933553e01de254f7e3 Mon Sep 17 00:00:00 2001 From: torossee Date: Wed, 20 Mar 2024 12:10:12 +0100 Subject: [PATCH 21/64] remove hardcode paths --- .nf-core.yml | 1 + conf/test.config | 5 +++-- modules.json | 22 ++++++---------------- nextflow.config | 4 ++-- 4 files changed, 12 insertions(+), 20 deletions(-) diff --git a/.nf-core.yml b/.nf-core.yml index 4124271..5cf0436 100644 --- a/.nf-core.yml +++ b/.nf-core.yml @@ -13,6 +13,7 @@ lint: - docs/images/nf-core-exomecnv_logo_light.png - docs/images/nf-core-exomecnv_logo_dark.png - .github/ISSUE_TEMPLATE/bug_report.yml + - LICENSE multiqc_config: - report_comment nextflow_config: diff --git a/conf/test.config b/conf/test.config index e76a22d..7855ee8 100644 --- a/conf/test.config +++ b/conf/test.config @@ -29,7 +29,8 @@ params { fai = 'https://github.com/nf-cmgg/test-datasets/tree/main/data/genomics/homo_sapiens/genome/seq/GCA_000001405.15_GRCh38_full_plus_hs38d1_analysis_set_chr21.fna.fai' // Parameters - roi_auto = "/home/torossee/projects/nf-cmgg-exomecnv_references/Homo_sapiens.GRCh38.105.chr21_protein_coding_basic_sorted_merged_autosomal.bed" - roi_chrx = "/home/torossee/projects/nf-cmgg-exomecnv_references/Homo_sapiens.GRCh38.105.chrX_protein_coding_basic_sorted_merged.bed" + roi_auto = "https://github.com/nf-cmgg/test-datasets/tree/main/data/genomics/homo_sapiens/genome/exomedepth/Homo_sapiens.GRCh38.105.chr21_protein_coding_basic_sorted_merged_autosomal.bed" + // roi_auto = "https://github.com/nf-cmgg/test-datasets/tree/main/data/genomics/homo_sapiens/genome/exomedepth/Homo_sapiens.GRCh38.105.chr_protein_coding_basic_sorted_merged_autosomal.bed" + roi_chrx = "https://github.com/nf-cmgg/test-datasets/tree/main/data/genomics/homo_sapiens/genome/exomedepth/Homo_sapiens.GRCh38.105.chrX_protein_coding_basic_sorted_merged.bed" } diff --git a/modules.json b/modules.json index 59147b2..0dbc9a9 100644 --- a/modules.json +++ b/modules.json @@ -8,16 +8,12 @@ "multiqc": { "branch": "master", "git_sha": "b7ebe95761cd389603f9cc0e0dc384c0f663815a", - "installed_by": [ - "modules" - ] + "installed_by": ["modules"] }, "samtools/convert": { "branch": "master", "git_sha": "1b93e06140e6807cc408519759f68bd26da63a00", - "installed_by": [ - "modules" - ] + "installed_by": ["modules"] } } }, @@ -26,26 +22,20 @@ "utils_nextflow_pipeline": { "branch": "master", "git_sha": "5caf7640a9ef1d18d765d55339be751bb0969dfa", - "installed_by": [ - "subworkflows" - ] + "installed_by": ["subworkflows"] }, "utils_nfcore_pipeline": { "branch": "master", "git_sha": "5caf7640a9ef1d18d765d55339be751bb0969dfa", - "installed_by": [ - "subworkflows" - ] + "installed_by": ["subworkflows"] }, "utils_nfvalidation_plugin": { "branch": "master", "git_sha": "5caf7640a9ef1d18d765d55339be751bb0969dfa", - "installed_by": [ - "subworkflows" - ] + "installed_by": ["subworkflows"] } } } } } -} \ No newline at end of file +} diff --git a/nextflow.config b/nextflow.config index d985c8e..f22b5b9 100644 --- a/nextflow.config +++ b/nextflow.config @@ -60,8 +60,8 @@ params { validate_params = true // ExomeDepth Parameters - roi_auto = "/home/torossee/projects/nf-cmgg-exomecnv_references/Homo_sapiens.GRCh38.105.chr_protein_coding_basic_sorted_merged_autosomal.bed" - roi_chrx = "/home/torossee/projects/nf-cmgg-exomecnv_references/Homo_sapiens.GRCh38.105.chrX_protein_coding_basic_sorted_merged.bed" + roi_auto = null + roi_chrx = null } From 57cb91c88bd63e654abbc0aeb0fa5563a93c8b04 Mon Sep 17 00:00:00 2001 From: torossee Date: Wed, 20 Mar 2024 13:28:26 +0100 Subject: [PATCH 22/64] small fixes --- bin/ExomeDepth_count.R | 14 +++++++------- conf/test.config | 2 +- main.nf | 2 +- nextflow_schema.json | 4 ++-- .../local/utils_nfcore_exomecnv_pipeline/main.nf | 4 +--- workflows/exomecnv.nf | 4 ++-- 6 files changed, 14 insertions(+), 16 deletions(-) diff --git a/bin/ExomeDepth_count.R b/bin/ExomeDepth_count.R index 8cf5d0a..07f6d8f 100755 --- a/bin/ExomeDepth_count.R +++ b/bin/ExomeDepth_count.R @@ -5,7 +5,7 @@ ############################################################################################ ### load libs ### -# library("optparse") +# library("optparse") library("methods") # to load the function "as" library("ExomeDepth") ExomeDepthVersion <- packageDescription("ExomeDepth")$Version @@ -15,11 +15,11 @@ print(paste0("...loaded ExomeDepth version: ", ExomeDepthVersion)) ## pass options ### args = commandArgs(trailingOnly = TRUE) print_usage <- function() { - cat("Usage: ExomeDepth_count.R \n") + cat("Usage: ExomeDepth_count.R \n") } if (length(commandArgs(trailingOnly = TRUE)) != 5) { - print_usage() - q("no", status = 1) # Terminate the script with an error status + print_usage() + q("no", status = 1) # Terminate the script with an error status } cat("samplename: ", args[1], "\n") @@ -61,12 +61,12 @@ ExomeCount <- getBamCounts(bed.frame = exons, cat("\nCounting done...\n") colnames(ExomeCount) ## convert GRanges class (S4 object) into a data.frame, which is the input format for ExomeDepth -ExomeCount.dafr <- as(ExomeCount[, colnames(ExomeCount)], 'data.frame') +ExomeCount.dafr <- as(ExomeCount[, colnames(ExomeCount)], 'data.frame') cat("\nConversion done...\n") print(head(ExomeCount.dafr)) ## remove the annoying chr letters ExomeCount.dafr$chromosome <- gsub(as.character(ExomeCount.dafr$chromosome), - pattern = 'chr', + pattern = 'chr', replacement = '') cat("\nChr's removed...\n") ## rename sample names in colnames @@ -79,7 +79,7 @@ cat("Successfully calculated counts.\n") ### save counts as a text file and rda object ### cat("Saving the counts \n") countspath = paste("counts_",sampleName,"_",args[5],".txt",sep="") -write.table(ExomeCount.dafr,countspath,sep="\t",col.names=TRUE,row.names=FALSE,quote=FALSE) +write.table(ExomeCount.dafr,countspath,sep="\t",col.names=TRUE,row.names=FALSE,quote=FALSE) cat("\n\n---Finished---\n") diff --git a/conf/test.config b/conf/test.config index 7855ee8..e9fd631 100644 --- a/conf/test.config +++ b/conf/test.config @@ -27,7 +27,7 @@ params { // Genome references fasta = 'https://github.com/nf-cmgg/test-datasets/tree/main/data/genomics/homo_sapiens/genome/seq/GCA_000001405.15_GRCh38_full_plus_hs38d1_analysis_set_chr21.fna' fai = 'https://github.com/nf-cmgg/test-datasets/tree/main/data/genomics/homo_sapiens/genome/seq/GCA_000001405.15_GRCh38_full_plus_hs38d1_analysis_set_chr21.fna.fai' - + // Parameters roi_auto = "https://github.com/nf-cmgg/test-datasets/tree/main/data/genomics/homo_sapiens/genome/exomedepth/Homo_sapiens.GRCh38.105.chr21_protein_coding_basic_sorted_merged_autosomal.bed" // roi_auto = "https://github.com/nf-cmgg/test-datasets/tree/main/data/genomics/homo_sapiens/genome/exomedepth/Homo_sapiens.GRCh38.105.chr_protein_coding_basic_sorted_merged_autosomal.bed" diff --git a/main.nf b/main.nf index 472c14e..f8aac4f 100644 --- a/main.nf +++ b/main.nf @@ -14,7 +14,7 @@ nextflow.enable.dsl = 2 GENOME PARAMETER VALUES ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ */ -// use getGenomeAttribute() to fetch parameters +// use getGenomeAttribute() to fetch parameters // from igenomes.config using `--genome` include { getGenomeAttribute } from './subworkflows/local/utils_nfcore_exomecnv_pipeline' diff --git a/nextflow_schema.json b/nextflow_schema.json index ec2b6eb..7ed9948 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -298,7 +298,7 @@ "format": "file-path", "pattern": "^\\S+\\.bed$", "mimetype": "text/plain", - "default": "/home/torossee/projects/nf-cmgg-exomecnv_references/Homo_sapiens.GRCh38.105.chr_protein_coding_basic_sorted_merged_autosomal.bed" + "default": null }, "roi_chrx": { "type": "string", @@ -306,7 +306,7 @@ "format": "file-path", "pattern": "^\\S+\\.bed$", "mimetype": "text/plain", - "default": "/home/torossee/projects/nf-cmgg-exomecnv_references/Homo_sapiens.GRCh38.105.chrX_protein_coding_basic_sorted_merged.bed" + "default": null } }, "required": ["roi_auto", "roi_chrx"] diff --git a/subworkflows/local/utils_nfcore_exomecnv_pipeline/main.nf b/subworkflows/local/utils_nfcore_exomecnv_pipeline/main.nf index 4fc5c8e..5204e0d 100644 --- a/subworkflows/local/utils_nfcore_exomecnv_pipeline/main.nf +++ b/subworkflows/local/utils_nfcore_exomecnv_pipeline/main.nf @@ -36,9 +36,7 @@ workflow PIPELINE_INITIALISATION { nextflow_cli_args // array: List of positional nextflow CLI args outdir // string: The output directory where the results will be saved input // string: Path to input samplesheet - roi_auto // string: Path to ExomeDepth BED file of (merged) autosomal regions - roi_chrx // string: Path to ExomeDepth BED file of (merged) chrX regions - + main: ch_versions = Channel.empty() diff --git a/workflows/exomecnv.nf b/workflows/exomecnv.nf index ed86ca6..3b653c5 100644 --- a/workflows/exomecnv.nf +++ b/workflows/exomecnv.nf @@ -43,12 +43,12 @@ workflow EXOMECNV { // ch_input_prepare.BAM.view { "BAM: $it" } // ch_input_prepare.CRAM.view { "CRAM: $it" } - + ch_fasta = Channel.fromPath(params.fasta).map{ [[id:"reference"], it]}.collect() ch_fai = params.fai ? Channel.fromPath(params.fai).map{ [[id:"reference"], it]}.collect() : null ch_roi_auto = Channel.fromPath(params.roi_auto).map{ [[id:"autosomal"], it]}.collect() ch_roi_x = Channel.fromPath(params.roi_chrx).map{ [[id:"chrX"], it]}.collect() - + // ch_roi_auto.view() // ch_roi_auto.view() From 563d1d2a2f0d380de20a9fe32fed3089d1b18560 Mon Sep 17 00:00:00 2001 From: torossee Date: Wed, 20 Mar 2024 13:31:12 +0100 Subject: [PATCH 23/64] small fix --- subworkflows/local/utils_nfcore_exomecnv_pipeline/main.nf | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/subworkflows/local/utils_nfcore_exomecnv_pipeline/main.nf b/subworkflows/local/utils_nfcore_exomecnv_pipeline/main.nf index 5204e0d..8c8b7a2 100644 --- a/subworkflows/local/utils_nfcore_exomecnv_pipeline/main.nf +++ b/subworkflows/local/utils_nfcore_exomecnv_pipeline/main.nf @@ -36,7 +36,7 @@ workflow PIPELINE_INITIALISATION { nextflow_cli_args // array: List of positional nextflow CLI args outdir // string: The output directory where the results will be saved input // string: Path to input samplesheet - + main: ch_versions = Channel.empty() From 02258949e6ab1080e0b587dd36129935787b8f9f Mon Sep 17 00:00:00 2001 From: torossee Date: Wed, 20 Mar 2024 14:35:18 +0100 Subject: [PATCH 24/64] fix test samplesheet --- assets/samplesheet.csv | 5 ++--- conf/test.config | 4 +--- 2 files changed, 3 insertions(+), 6 deletions(-) diff --git a/assets/samplesheet.csv b/assets/samplesheet.csv index 2bbdf0e..5e722b5 100644 --- a/assets/samplesheet.csv +++ b/assets/samplesheet.csv @@ -1,4 +1,3 @@ sample,pool,family,cram,crai -DNA049572,poolF,Proband_24.03390,/path/to/cram/files/test.cram,/path/to/cram/files/test.cram.crai -NA24385D2_NVQ_034,poolM,Proband_24.03390,/path/to/cram/files/test2.cram,/path/to/cram/files/test2.cram.crai - +DNA049572,poolF,Proband_24.03390,https://github.com/nf-cmgg/test-datasets/tree/main/data/genomics/homo_sapiens/illumina/cram/cram/test.cram,https://github.com/nf-cmgg/test-datasets/tree/main/data/genomics/homo_sapiens/illumina/cram/test.cram.crai +NA24385D2_NVQ_034,poolM,Proband_24.03390,https://github.com/nf-cmgg/test-datasets/tree/main/data/genomics/homo_sapiens/illumina/cram/test2.cram,https://github.com/nf-cmgg/test-datasets/tree/main/data/genomics/homo_sapiens/illumina/cram/test2.cram.crai diff --git a/conf/test.config b/conf/test.config index e9fd631..2307033 100644 --- a/conf/test.config +++ b/conf/test.config @@ -20,9 +20,7 @@ params { max_time = '6.h' // Input data - // TODO nf-core: Specify the paths to your test data on nf-core/test-datasets - // TODO nf-core: Give any required params for the test so that command line flags are not needed - input = 'https://raw.githubusercontent.com/nf-core/test-datasets/viralrecon/samplesheet/samplesheet_test_illumina_amplicon.csv' + input = "${projectDir}/assets/samplesheet.csv" // Genome references fasta = 'https://github.com/nf-cmgg/test-datasets/tree/main/data/genomics/homo_sapiens/genome/seq/GCA_000001405.15_GRCh38_full_plus_hs38d1_analysis_set_chr21.fna' From c84fb981402d8a6b3299a76ec29eb61049592fb8 Mon Sep 17 00:00:00 2001 From: torossee Date: Wed, 20 Mar 2024 14:44:27 +0100 Subject: [PATCH 25/64] good formatting --- CHANGELOG.md | 1 + assets/schema_input.json | 2 +- 2 files changed, 2 insertions(+), 1 deletion(-) diff --git a/CHANGELOG.md b/CHANGELOG.md index a5cac08..885bf91 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -8,6 +8,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0 Initial release of nf-cmgg/exomecnv, created with the [nf-core](https://nf-co.re/) template. ### `Changes` + - initiate step 1: CRAM -> BAM - new repo name `nf-cmgg/exomecnv` - Upgraded to nf-core v2.13 template diff --git a/assets/schema_input.json b/assets/schema_input.json index 66620e6..d4ce515 100644 --- a/assets/schema_input.json +++ b/assets/schema_input.json @@ -40,6 +40,6 @@ "exists": true } }, - "required": ["sample", "pool","family","cram"] + "required": ["sample", "pool", "family", "cram"] } } From d0cca5aa86634e920c2fecf9baad04599e1da087 Mon Sep 17 00:00:00 2001 From: torossee Date: Wed, 20 Mar 2024 14:46:01 +0100 Subject: [PATCH 26/64] fix --- assets/samplesheet.csv | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/assets/samplesheet.csv b/assets/samplesheet.csv index 5e722b5..560c7a5 100644 --- a/assets/samplesheet.csv +++ b/assets/samplesheet.csv @@ -1,3 +1,3 @@ sample,pool,family,cram,crai -DNA049572,poolF,Proband_24.03390,https://github.com/nf-cmgg/test-datasets/tree/main/data/genomics/homo_sapiens/illumina/cram/cram/test.cram,https://github.com/nf-cmgg/test-datasets/tree/main/data/genomics/homo_sapiens/illumina/cram/test.cram.crai +DNA049572,poolF,Proband_24.03390,https://github.com/nf-cmgg/test-datasets/tree/main/data/genomics/homo_sapiens/illumina/cram/test.cram,https://github.com/nf-cmgg/test-datasets/tree/main/data/genomics/homo_sapiens/illumina/cram/test.cram.crai NA24385D2_NVQ_034,poolM,Proband_24.03390,https://github.com/nf-cmgg/test-datasets/tree/main/data/genomics/homo_sapiens/illumina/cram/test2.cram,https://github.com/nf-cmgg/test-datasets/tree/main/data/genomics/homo_sapiens/illumina/cram/test2.cram.crai From c824104c85fc8232b334c5d2526954b04e6dc320 Mon Sep 17 00:00:00 2001 From: torossee Date: Wed, 20 Mar 2024 15:38:13 +0100 Subject: [PATCH 27/64] fix file paths --- assets/samplesheet.csv | 4 ++-- conf/test.config | 10 +++++----- modules/local/exomedepth/count/main.nf | 2 +- subworkflows/local/exomedepth_count/main.nf | 2 +- 4 files changed, 9 insertions(+), 9 deletions(-) diff --git a/assets/samplesheet.csv b/assets/samplesheet.csv index 560c7a5..620385a 100644 --- a/assets/samplesheet.csv +++ b/assets/samplesheet.csv @@ -1,3 +1,3 @@ sample,pool,family,cram,crai -DNA049572,poolF,Proband_24.03390,https://github.com/nf-cmgg/test-datasets/tree/main/data/genomics/homo_sapiens/illumina/cram/test.cram,https://github.com/nf-cmgg/test-datasets/tree/main/data/genomics/homo_sapiens/illumina/cram/test.cram.crai -NA24385D2_NVQ_034,poolM,Proband_24.03390,https://github.com/nf-cmgg/test-datasets/tree/main/data/genomics/homo_sapiens/illumina/cram/test2.cram,https://github.com/nf-cmgg/test-datasets/tree/main/data/genomics/homo_sapiens/illumina/cram/test2.cram.crai +DNA049572,poolF,Proband_24.03390,https://raw.githubusercontent.com/nf-cmgg/test-datasets/main/data/genomics/homo_sapiens/illumina/cram/test.cram,https://raw.githubusercontent.com/nf-cmgg/test-datasets/main/data/genomics/homo_sapiens/illumina/cram/test.cram.crai +NA24385D2_NVQ_034,poolM,Proband_24.03390,https://raw.githubusercontent.com/nf-cmgg/test-datasets/main/data/genomics/homo_sapiens/illumina/cram/test2.cram,https://raw.githubusercontent.com/nf-cmgg/test-datasets/main/data/genomics/homo_sapiens/illumina/cram/test2.cram.crai diff --git a/conf/test.config b/conf/test.config index 2307033..4232eb3 100644 --- a/conf/test.config +++ b/conf/test.config @@ -23,12 +23,12 @@ params { input = "${projectDir}/assets/samplesheet.csv" // Genome references - fasta = 'https://github.com/nf-cmgg/test-datasets/tree/main/data/genomics/homo_sapiens/genome/seq/GCA_000001405.15_GRCh38_full_plus_hs38d1_analysis_set_chr21.fna' - fai = 'https://github.com/nf-cmgg/test-datasets/tree/main/data/genomics/homo_sapiens/genome/seq/GCA_000001405.15_GRCh38_full_plus_hs38d1_analysis_set_chr21.fna.fai' + fasta = 'https://raw.githubusercontent.com/nf-cmgg/test-datasets/main/data/genomics/homo_sapiens/genome/seq/GCA_000001405.15_GRCh38_full_plus_hs38d1_analysis_set_chr21.fna' + fai = 'https://raw.githubusercontent.com/nf-cmgg/test-datasets/main/data/genomics/homo_sapiens/genome/seq/GCA_000001405.15_GRCh38_full_plus_hs38d1_analysis_set_chr21.fna.fai' // Parameters - roi_auto = "https://github.com/nf-cmgg/test-datasets/tree/main/data/genomics/homo_sapiens/genome/exomedepth/Homo_sapiens.GRCh38.105.chr21_protein_coding_basic_sorted_merged_autosomal.bed" - // roi_auto = "https://github.com/nf-cmgg/test-datasets/tree/main/data/genomics/homo_sapiens/genome/exomedepth/Homo_sapiens.GRCh38.105.chr_protein_coding_basic_sorted_merged_autosomal.bed" - roi_chrx = "https://github.com/nf-cmgg/test-datasets/tree/main/data/genomics/homo_sapiens/genome/exomedepth/Homo_sapiens.GRCh38.105.chrX_protein_coding_basic_sorted_merged.bed" + roi_auto = "https://raw.githubusercontent.com/nf-cmgg/test-datasets/main/data/genomics/homo_sapiens/genome/exomedepth/Homo_sapiens.GRCh38.105.chr21_protein_coding_basic_sorted_merged_autosomal.bed" + // roi_auto = "https://raw.githubusercontent.com/nf-cmgg/test-datasets/main/data/genomics/homo_sapiens/genome/exomedepth/Homo_sapiens.GRCh38.105.chr_protein_coding_basic_sorted_merged_autosomal.bed" + roi_chrx = "https://raw.githubusercontent.com/nf-cmgg/test-datasets/main/data/genomics/homo_sapiens/genome/exomedepth/Homo_sapiens.GRCh38.105.chrX_protein_coding_basic_sorted_merged.bed" } diff --git a/modules/local/exomedepth/count/main.nf b/modules/local/exomedepth/count/main.nf index b0d96fe..31bf183 100644 --- a/modules/local/exomedepth/count/main.nf +++ b/modules/local/exomedepth/count/main.nf @@ -1,5 +1,5 @@ process COUNT { - tag "$meta.id $prefix.id" + tag "$meta.id $meta2.id" label 'process_low' conda "${moduleDir}/environment.yml" diff --git a/subworkflows/local/exomedepth_count/main.nf b/subworkflows/local/exomedepth_count/main.nf index 9466417..991f310 100644 --- a/subworkflows/local/exomedepth_count/main.nf +++ b/subworkflows/local/exomedepth_count/main.nf @@ -6,7 +6,7 @@ workflow EXOMEDEPTH_COUNT { take: ch_bam // channel: [mandatory] [ val(meta), path(bam), path(bai) ] - exon_target // channel: [mandatory] [ val(prefix), path(bed) ] + exon_target // channel: [mandatory] [ val(meta2), path(bed) ] main: From 4d4164e954742b65ee8eb7711fd9ae6f6177dac9 Mon Sep 17 00:00:00 2001 From: torossee Date: Tue, 16 Apr 2024 14:55:46 +0200 Subject: [PATCH 28/64] count chrX + new test cram + restructure --- assets/samplesheet.csv | 12 ++++++++++-- assets/samplesheet_bam.csv | 11 +++++++++++ bin/ExomeDepth_count.R | 2 +- conf/modules.config | 7 +++++++ conf/test.config | 12 +++++++----- workflows/exomecnv.nf | 21 ++++++++++++++++----- 6 files changed, 52 insertions(+), 13 deletions(-) create mode 100644 assets/samplesheet_bam.csv diff --git a/assets/samplesheet.csv b/assets/samplesheet.csv index 620385a..d25fe22 100644 --- a/assets/samplesheet.csv +++ b/assets/samplesheet.csv @@ -1,3 +1,11 @@ sample,pool,family,cram,crai -DNA049572,poolF,Proband_24.03390,https://raw.githubusercontent.com/nf-cmgg/test-datasets/main/data/genomics/homo_sapiens/illumina/cram/test.cram,https://raw.githubusercontent.com/nf-cmgg/test-datasets/main/data/genomics/homo_sapiens/illumina/cram/test.cram.crai -NA24385D2_NVQ_034,poolM,Proband_24.03390,https://raw.githubusercontent.com/nf-cmgg/test-datasets/main/data/genomics/homo_sapiens/illumina/cram/test2.cram,https://raw.githubusercontent.com/nf-cmgg/test-datasets/main/data/genomics/homo_sapiens/illumina/cram/test2.cram.crai +Sample1,poolM,Fam1,https://raw.githubusercontent.com/nf-cmgg/test-datasets/exomecnv/data/genomics/homo_sapiens/illumina/cram/Sample1.cram,https://raw.githubusercontent.com/nf-cmgg/test-datasets/exomecnv/data/genomics/homo_sapiens/illumina/cram/Sample1.cram.crai +Sample2,poolM,Fam2,https://raw.githubusercontent.com/nf-cmgg/test-datasets/exomecnv/data/genomics/homo_sapiens/illumina/cram/Sample2.cram,https://raw.githubusercontent.com/nf-cmgg/test-datasets/exomecnv/data/genomics/homo_sapiens/illumina/cram/Sample2.cram.crai +Sample3,poolM,Fam3,https://raw.githubusercontent.com/nf-cmgg/test-datasets/exomecnv/data/genomics/homo_sapiens/illumina/cram/Sample3.cram,https://raw.githubusercontent.com/nf-cmgg/test-datasets/exomecnv/data/genomics/homo_sapiens/illumina/cram/Sample3.cram.crai +Sample4,poolM,Fam4,https://raw.githubusercontent.com/nf-cmgg/test-datasets/exomecnv/data/genomics/homo_sapiens/illumina/cram/Sample4.cram,https://raw.githubusercontent.com/nf-cmgg/test-datasets/exomecnv/data/genomics/homo_sapiens/illumina/cram/Sample4.cram.crai +Sample5,poolM,Fam5,https://raw.githubusercontent.com/nf-cmgg/test-datasets/exomecnv/data/genomics/homo_sapiens/illumina/cram/Sample5.cram,https://raw.githubusercontent.com/nf-cmgg/test-datasets/exomecnv/data/genomics/homo_sapiens/illumina/cram/Sample5.cram.crai +Sample6,poolF,Fam6,https://raw.githubusercontent.com/nf-cmgg/test-datasets/exomecnv/data/genomics/homo_sapiens/illumina/cram/Sample6.cram,https://raw.githubusercontent.com/nf-cmgg/test-datasets/exomecnv/data/genomics/homo_sapiens/illumina/cram/Sample6.cram.crai +Sample7,poolF,Fam7,https://raw.githubusercontent.com/nf-cmgg/test-datasets/exomecnv/data/genomics/homo_sapiens/illumina/cram/Sample7.cram,https://raw.githubusercontent.com/nf-cmgg/test-datasets/exomecnv/data/genomics/homo_sapiens/illumina/cram/Sample7.cram.crai +Sample8,poolF,Fam8,https://raw.githubusercontent.com/nf-cmgg/test-datasets/exomecnv/data/genomics/homo_sapiens/illumina/cram/Sample8.cram,https://raw.githubusercontent.com/nf-cmgg/test-datasets/exomecnv/data/genomics/homo_sapiens/illumina/cram/Sample8.cram.crai +Sample9,poolF,Fam9,https://raw.githubusercontent.com/nf-cmgg/test-datasets/exomecnv/data/genomics/homo_sapiens/illumina/cram/Sample9.cram,https://raw.githubusercontent.com/nf-cmgg/test-datasets/exomecnv/data/genomics/homo_sapiens/illumina/cram/Sample9.cram.crai +Sample10,poolF,Fam9,https://raw.githubusercontent.com/nf-cmgg/test-datasets/exomecnv/data/genomics/homo_sapiens/illumina/cram/Sample10.cram,https://raw.githubusercontent.com/nf-cmgg/test-datasets/exomecnv/data/genomics/homo_sapiens/illumina/cram/Sample10.cram.crai diff --git a/assets/samplesheet_bam.csv b/assets/samplesheet_bam.csv new file mode 100644 index 0000000..5202a6d --- /dev/null +++ b/assets/samplesheet_bam.csv @@ -0,0 +1,11 @@ +sample,pool,family,cram,crai +Sample1,poolM,Fam1,https://raw.githubusercontent.com/nf-cmgg/test-datasets/exomecnv/data/genomics/homo_sapiens/illumina/bam/Sample1.bam,https://raw.githubusercontent.com/nf-cmgg/test-datasets/exomecnv/data/genomics/homo_sapiens/illumina/bam/Sample1.bam.bai +Sample2,poolM,Fam2,https://raw.githubusercontent.com/nf-cmgg/test-datasets/exomecnv/data/genomics/homo_sapiens/illumina/bam/Sample2.bam,https://raw.githubusercontent.com/nf-cmgg/test-datasets/exomecnv/data/genomics/homo_sapiens/illumina/bam/Sample2.bam.bai +Sample3,poolM,Fam3,https://raw.githubusercontent.com/nf-cmgg/test-datasets/exomecnv/data/genomics/homo_sapiens/illumina/bam/Sample3.bam,https://raw.githubusercontent.com/nf-cmgg/test-datasets/exomecnv/data/genomics/homo_sapiens/illumina/bam/Sample3.bam.bai +Sample4,poolM,Fam4,https://raw.githubusercontent.com/nf-cmgg/test-datasets/exomecnv/data/genomics/homo_sapiens/illumina/bam/Sample4.bam,https://raw.githubusercontent.com/nf-cmgg/test-datasets/exomecnv/data/genomics/homo_sapiens/illumina/bam/Sample4.bam.bai +Sample5,poolM,Fam5,https://raw.githubusercontent.com/nf-cmgg/test-datasets/exomecnv/data/genomics/homo_sapiens/illumina/bam/Sample5.bam,https://raw.githubusercontent.com/nf-cmgg/test-datasets/exomecnv/data/genomics/homo_sapiens/illumina/bam/Sample5.bam.bai +Sample6,poolF,Fam6,https://raw.githubusercontent.com/nf-cmgg/test-datasets/exomecnv/data/genomics/homo_sapiens/illumina/bam/Sample6.bam,https://raw.githubusercontent.com/nf-cmgg/test-datasets/exomecnv/data/genomics/homo_sapiens/illumina/bam/Sample6.bam.bai +Sample7,poolF,Fam7,https://raw.githubusercontent.com/nf-cmgg/test-datasets/exomecnv/data/genomics/homo_sapiens/illumina/bam/Sample7.bam,https://raw.githubusercontent.com/nf-cmgg/test-datasets/exomecnv/data/genomics/homo_sapiens/illumina/bam/Sample7.bam.bai +Sample8,poolF,Fam8,https://raw.githubusercontent.com/nf-cmgg/test-datasets/exomecnv/data/genomics/homo_sapiens/illumina/bam/Sample8.bam,https://raw.githubusercontent.com/nf-cmgg/test-datasets/exomecnv/data/genomics/homo_sapiens/illumina/bam/Sample8.bam.bai +Sample9,poolF,Fam9,https://raw.githubusercontent.com/nf-cmgg/test-datasets/exomecnv/data/genomics/homo_sapiens/illumina/bam/Sample9.bam,https://raw.githubusercontent.com/nf-cmgg/test-datasets/exomecnv/data/genomics/homo_sapiens/illumina/bam/Sample9.bam.bai +Sample10,poolF,Fam9,https://raw.githubusercontent.com/nf-cmgg/test-datasets/exomecnv/data/genomics/homo_sapiens/illumina/bam/Sample10.bam,https://raw.githubusercontent.com/nf-cmgg/test-datasets/exomecnv/data/genomics/homo_sapiens/illumina/bam/Sample10.bam.bai diff --git a/bin/ExomeDepth_count.R b/bin/ExomeDepth_count.R index 07f6d8f..ae8ded6 100755 --- a/bin/ExomeDepth_count.R +++ b/bin/ExomeDepth_count.R @@ -78,7 +78,7 @@ cat("Successfully calculated counts.\n") ### save counts as a text file and rda object ### cat("Saving the counts \n") -countspath = paste("counts_",sampleName,"_",args[5],".txt",sep="") +countspath = paste(sampleName,"_",args[5],".txt",sep="") write.table(ExomeCount.dafr,countspath,sep="\t",col.names=TRUE,row.names=FALSE,quote=FALSE) cat("\n\n---Finished---\n") diff --git a/conf/modules.config b/conf/modules.config index e3ea8fa..800f8fc 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -39,4 +39,11 @@ process { ] } + withName: 'CRAM_TO_BAM' { + publishDir = [ + path: { "${params.outdir}/bam" }, + mode: params.publish_dir_mode, + saveAs: { filename -> filename.equals('versions.yml') ? null : filename } + ] + } } diff --git a/conf/test.config b/conf/test.config index 4232eb3..0da3a97 100644 --- a/conf/test.config +++ b/conf/test.config @@ -20,15 +20,17 @@ params { max_time = '6.h' // Input data + // input = "${projectDir}/assets/samplesheet_bam.csv" input = "${projectDir}/assets/samplesheet.csv" // Genome references - fasta = 'https://raw.githubusercontent.com/nf-cmgg/test-datasets/main/data/genomics/homo_sapiens/genome/seq/GCA_000001405.15_GRCh38_full_plus_hs38d1_analysis_set_chr21.fna' - fai = 'https://raw.githubusercontent.com/nf-cmgg/test-datasets/main/data/genomics/homo_sapiens/genome/seq/GCA_000001405.15_GRCh38_full_plus_hs38d1_analysis_set_chr21.fna.fai' + fasta = 'https://raw.githubusercontent.com/nf-cmgg/test-datasets/exomecnv/data/genomics/homo_sapiens/genome/seq/GCA_000001405.15_GRCh38_full_plus_hs38d1_analysis_set_chr21_and_chrX_subset.fna' + fai = 'https://raw.githubusercontent.com/nf-cmgg/test-datasets/exomecnv/data/genomics/homo_sapiens/genome/seq/GCA_000001405.15_GRCh38_full_plus_hs38d1_analysis_set_chr21_and_chrX_subset.fna.fai' // Parameters - roi_auto = "https://raw.githubusercontent.com/nf-cmgg/test-datasets/main/data/genomics/homo_sapiens/genome/exomedepth/Homo_sapiens.GRCh38.105.chr21_protein_coding_basic_sorted_merged_autosomal.bed" - // roi_auto = "https://raw.githubusercontent.com/nf-cmgg/test-datasets/main/data/genomics/homo_sapiens/genome/exomedepth/Homo_sapiens.GRCh38.105.chr_protein_coding_basic_sorted_merged_autosomal.bed" - roi_chrx = "https://raw.githubusercontent.com/nf-cmgg/test-datasets/main/data/genomics/homo_sapiens/genome/exomedepth/Homo_sapiens.GRCh38.105.chrX_protein_coding_basic_sorted_merged.bed" + roi_auto = "https://raw.githubusercontent.com/nf-cmgg/test-datasets/exomecnv/data/genomics/homo_sapiens/genome/roi/Homo_sapiens.GRCh38.105.chr21_protein_coding_basic_sorted_merged_autosomal.bed" + roi_chrx = "https://raw.githubusercontent.com/nf-cmgg/test-datasets/exomecnv/data/genomics/homo_sapiens/genome/roi/Homo_sapiens.GRCh38.105.chrX_protein_coding_basic_sorted_merged.bed" } + + diff --git a/workflows/exomecnv.nf b/workflows/exomecnv.nf index 3b653c5..12bce66 100644 --- a/workflows/exomecnv.nf +++ b/workflows/exomecnv.nf @@ -12,7 +12,9 @@ include { methodsDescriptionText } from '../subworkflows/local/utils_nfcore_exom // local include { CRAM_PREPARE } from '../subworkflows/local/cram_prepare/main' -include { EXOMEDEPTH_COUNT } from '../subworkflows/local/exomedepth_count/main' +include { EXOMEDEPTH_COUNT as COUNT_X } from '../subworkflows/local/exomedepth_count/main' +include { EXOMEDEPTH_COUNT as COUNT_AUTO } from '../subworkflows/local/exomedepth_count/main' + // include { BAM_VARIANT_CALLING_EXOMEDEPTH } from '../subworkflows/local/bam_variant_calling_exomedepth/main' /* ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ @@ -49,6 +51,8 @@ workflow EXOMECNV { ch_roi_auto = Channel.fromPath(params.roi_auto).map{ [[id:"autosomal"], it]}.collect() ch_roi_x = Channel.fromPath(params.roi_chrx).map{ [[id:"chrX"], it]}.collect() + // ch_fasta.view() + // ch_fai.view() // ch_roi_auto.view() // ch_roi_auto.view() @@ -56,17 +60,24 @@ workflow EXOMECNV { CRAM_PREPARE ( ch_input_prepare.CRAM, ch_fasta, ch_fai ) + // ch_input_prepare.BAM.view{ "BAM: $it" } + // CRAM_PREPARE.out.bai.view{ "samtools: $it" } + CRAM_PREPARE.out.bam + .join(CRAM_PREPARE.out.bai) + .set{ ch_cram_prepare } ch_input_prepare.BAM - .mix(CRAM_PREPARE.out.bam) - .join(CRAM_PREPARE.out.bai) + .mix(ch_cram_prepare) .set{ ch_input_bam } // ch_input_bam.view() - - EXOMEDEPTH_COUNT ( + COUNT_AUTO ( ch_input_bam, ch_roi_auto ) + COUNT_X ( + ch_input_bam, ch_roi_x + ) + // // Collate and save software versions // From d290c7e3d090be7ba57657af2ebb7754c65407e1 Mon Sep 17 00:00:00 2001 From: BertGalle Date: Tue, 23 Apr 2024 13:39:35 +0200 Subject: [PATCH 29/64] Added merge count files per pool --- modules/local/exomedepth/merge_count/main.nf | 22 +++++++++++ workflows/exomecnv.nf | 39 +++++++++++++++++--- 2 files changed, 55 insertions(+), 6 deletions(-) create mode 100644 modules/local/exomedepth/merge_count/main.nf diff --git a/modules/local/exomedepth/merge_count/main.nf b/modules/local/exomedepth/merge_count/main.nf new file mode 100644 index 0000000..7cc2ac7 --- /dev/null +++ b/modules/local/exomedepth/merge_count/main.nf @@ -0,0 +1,22 @@ +// MERGE COUNT FILES +process EXOMEDEPTH_COUNT_MERGE { + publishDir "$params.outdir/exomedepth/counts", mode: 'copy' + + input: + tuple val(meta), path(files) + + output: + tuple val(meta), path("${meta.id}_${meta.chr}.txt") + + script: + """ + for file in $files; do + if [ -f ${meta.id}_${meta.chr}.txt ]; then + paste ${meta.id}_${meta.chr}.txt <(awk '{print \$5}' \$file) > temp_auto.txt + mv temp_auto.txt ${meta.id}_${meta.chr}.txt + else + cp \$file ${meta.id}_${meta.chr}.txt + fi + done + """ +} diff --git a/workflows/exomecnv.nf b/workflows/exomecnv.nf index 12bce66..475c07a 100644 --- a/workflows/exomecnv.nf +++ b/workflows/exomecnv.nf @@ -11,9 +11,12 @@ include { softwareVersionsToYAML } from '../subworkflows/nf-core/utils_nfcore_pi include { methodsDescriptionText } from '../subworkflows/local/utils_nfcore_exomecnv_pipeline' // local -include { CRAM_PREPARE } from '../subworkflows/local/cram_prepare/main' -include { EXOMEDEPTH_COUNT as COUNT_X } from '../subworkflows/local/exomedepth_count/main' -include { EXOMEDEPTH_COUNT as COUNT_AUTO } from '../subworkflows/local/exomedepth_count/main' +include { CRAM_PREPARE } from '../subworkflows/local/cram_prepare/main' +include { EXOMEDEPTH_COUNT as COUNT_X } from '../subworkflows/local/exomedepth_count/main' +include { EXOMEDEPTH_COUNT as COUNT_AUTO } from '../subworkflows/local/exomedepth_count/main' +include { EXOMEDEPTH_COUNT_MERGE as COUNT_MERGE_AUTO } from '../modules/local/exomedepth/merge_count/main' +include { EXOMEDEPTH_COUNT_MERGE as COUNT_MERGE_X } from '../modules/local/exomedepth/merge_count/main' + // include { BAM_VARIANT_CALLING_EXOMEDEPTH } from '../subworkflows/local/bam_variant_calling_exomedepth/main' /* @@ -42,8 +45,10 @@ workflow EXOMECNV { BAM: cram.extension == "bam" } .set{ ch_input_prepare } - // ch_input_prepare.BAM.view { "BAM: $it" } - // ch_input_prepare.CRAM.view { "CRAM: $it" } + + + //ch_input_prepare.BAM.view { "BAM: $it" } + //ch_input_prepare.CRAM.view { "CRAM: $it" } ch_fasta = Channel.fromPath(params.fasta).map{ [[id:"reference"], it]}.collect() @@ -74,10 +79,32 @@ workflow EXOMECNV { COUNT_AUTO ( ch_input_bam, ch_roi_auto ) + + grouped_counts_auto = COUNT_AUTO.out.count + .map { meta, txt -> + def new_meta = [id:meta.pool,chr:"autosomal"] + [new_meta, txt] + } + .groupTuple() + + COUNT_MERGE_AUTO ( + grouped_counts_auto + ) + COUNT_X ( ch_input_bam, ch_roi_x ) - + + grouped_counts_X = COUNT_X.out.count + .map { meta, txt -> + def new_meta = [id:meta.pool,chr:"chrX"] + [new_meta, txt] + } + .groupTuple() + + COUNT_MERGE_X ( + grouped_counts_X + ) // // Collate and save software versions // From df7e6224e50704e1e3e85952325932aa01d87c9b Mon Sep 17 00:00:00 2001 From: BertGalle Date: Tue, 23 Apr 2024 15:05:17 +0200 Subject: [PATCH 30/64] Merge count files --- modules/local/exomedepth/merge_count/main.nf | 10 +++++----- workflows/exomecnv.nf | 18 +++++++++--------- 2 files changed, 14 insertions(+), 14 deletions(-) diff --git a/modules/local/exomedepth/merge_count/main.nf b/modules/local/exomedepth/merge_count/main.nf index 7cc2ac7..771ee2c 100644 --- a/modules/local/exomedepth/merge_count/main.nf +++ b/modules/local/exomedepth/merge_count/main.nf @@ -1,22 +1,22 @@ -// MERGE COUNT FILES +// MERGE COUNT FILES process EXOMEDEPTH_COUNT_MERGE { publishDir "$params.outdir/exomedepth/counts", mode: 'copy' input: - tuple val(meta), path(files) - + tuple val(meta), path(files) + output: tuple val(meta), path("${meta.id}_${meta.chr}.txt") script: """ for file in $files; do - if [ -f ${meta.id}_${meta.chr}.txt ]; then + if [ -f ${meta.id}_${meta.chr}.txt ]; then paste ${meta.id}_${meta.chr}.txt <(awk '{print \$5}' \$file) > temp_auto.txt mv temp_auto.txt ${meta.id}_${meta.chr}.txt else cp \$file ${meta.id}_${meta.chr}.txt - fi + fi done """ } diff --git a/workflows/exomecnv.nf b/workflows/exomecnv.nf index 475c07a..a5968c3 100644 --- a/workflows/exomecnv.nf +++ b/workflows/exomecnv.nf @@ -15,7 +15,7 @@ include { CRAM_PREPARE } from '../subworkflows/loc include { EXOMEDEPTH_COUNT as COUNT_X } from '../subworkflows/local/exomedepth_count/main' include { EXOMEDEPTH_COUNT as COUNT_AUTO } from '../subworkflows/local/exomedepth_count/main' include { EXOMEDEPTH_COUNT_MERGE as COUNT_MERGE_AUTO } from '../modules/local/exomedepth/merge_count/main' -include { EXOMEDEPTH_COUNT_MERGE as COUNT_MERGE_X } from '../modules/local/exomedepth/merge_count/main' +include { EXOMEDEPTH_COUNT_MERGE as COUNT_MERGE_X } from '../modules/local/exomedepth/merge_count/main' // include { BAM_VARIANT_CALLING_EXOMEDEPTH } from '../subworkflows/local/bam_variant_calling_exomedepth/main' @@ -45,7 +45,7 @@ workflow EXOMECNV { BAM: cram.extension == "bam" } .set{ ch_input_prepare } - + //ch_input_prepare.BAM.view { "BAM: $it" } //ch_input_prepare.CRAM.view { "CRAM: $it" } @@ -79,7 +79,7 @@ workflow EXOMECNV { COUNT_AUTO ( ch_input_bam, ch_roi_auto ) - + grouped_counts_auto = COUNT_AUTO.out.count .map { meta, txt -> def new_meta = [id:meta.pool,chr:"autosomal"] @@ -88,14 +88,14 @@ workflow EXOMECNV { .groupTuple() COUNT_MERGE_AUTO ( - grouped_counts_auto - ) - + grouped_counts_auto + ) + COUNT_X ( ch_input_bam, ch_roi_x ) - - grouped_counts_X = COUNT_X.out.count + + grouped_counts_X = COUNT_X.out.count .map { meta, txt -> def new_meta = [id:meta.pool,chr:"chrX"] [new_meta, txt] @@ -103,7 +103,7 @@ workflow EXOMECNV { .groupTuple() COUNT_MERGE_X ( - grouped_counts_X + grouped_counts_X ) // // Collate and save software versions From 5bd91ebac94c16c4e491ca7b8278ce858b64d6f9 Mon Sep 17 00:00:00 2001 From: BertGalle Date: Tue, 23 Apr 2024 15:23:14 +0200 Subject: [PATCH 31/64] Merge counts per pool --- modules/local/exomedepth/merge_count/main.nf | 8 ++++---- workflows/exomecnv.nf | 4 ++-- 2 files changed, 6 insertions(+), 6 deletions(-) diff --git a/modules/local/exomedepth/merge_count/main.nf b/modules/local/exomedepth/merge_count/main.nf index 771ee2c..333652b 100644 --- a/modules/local/exomedepth/merge_count/main.nf +++ b/modules/local/exomedepth/merge_count/main.nf @@ -12,10 +12,10 @@ process EXOMEDEPTH_COUNT_MERGE { """ for file in $files; do if [ -f ${meta.id}_${meta.chr}.txt ]; then - paste ${meta.id}_${meta.chr}.txt <(awk '{print \$5}' \$file) > temp_auto.txt - mv temp_auto.txt ${meta.id}_${meta.chr}.txt - else - cp \$file ${meta.id}_${meta.chr}.txt + paste ${meta.id}_${meta.chr}.txt <(awk '{print \$5}' \$file) > temp_auto.txt + mv temp_auto.txt ${meta.id}_${meta.chr}.txt + else + cp \$file ${meta.id}_${meta.chr}.txt fi done """ diff --git a/workflows/exomecnv.nf b/workflows/exomecnv.nf index a5968c3..d39b913 100644 --- a/workflows/exomecnv.nf +++ b/workflows/exomecnv.nf @@ -47,8 +47,8 @@ workflow EXOMECNV { .set{ ch_input_prepare } - //ch_input_prepare.BAM.view { "BAM: $it" } - //ch_input_prepare.CRAM.view { "CRAM: $it" } + //ch_input_prepare.BAM.view { "BAM: $it" } + //ch_input_prepare.CRAM.view { "CRAM: $it" } ch_fasta = Channel.fromPath(params.fasta).map{ [[id:"reference"], it]}.collect() From c549bde8c469f81e89f98243efba4abe26983e14 Mon Sep 17 00:00:00 2001 From: BertGalle Date: Tue, 23 Apr 2024 15:25:15 +0200 Subject: [PATCH 32/64] Merge count per pool --- modules/local/exomedepth/merge_count/main.nf | 8 ++++---- 1 file changed, 4 insertions(+), 4 deletions(-) diff --git a/modules/local/exomedepth/merge_count/main.nf b/modules/local/exomedepth/merge_count/main.nf index 333652b..a7e0bec 100644 --- a/modules/local/exomedepth/merge_count/main.nf +++ b/modules/local/exomedepth/merge_count/main.nf @@ -12,10 +12,10 @@ process EXOMEDEPTH_COUNT_MERGE { """ for file in $files; do if [ -f ${meta.id}_${meta.chr}.txt ]; then - paste ${meta.id}_${meta.chr}.txt <(awk '{print \$5}' \$file) > temp_auto.txt - mv temp_auto.txt ${meta.id}_${meta.chr}.txt - else - cp \$file ${meta.id}_${meta.chr}.txt + paste ${meta.id}_${meta.chr}.txt <(awk '{print \$5}' \$file) > temp_auto.txt + mv temp_auto.txt ${meta.id}_${meta.chr}.txt + else + cp \$file ${meta.id}_${meta.chr}.txt fi done """ From fca9d5dfba51da2a299b1b2706ff1327efdeaa90 Mon Sep 17 00:00:00 2001 From: BertGalle <129084478+BertGalle@users.noreply.github.com> Date: Tue, 23 Apr 2024 15:42:28 +0200 Subject: [PATCH 33/64] Update modules/local/exomedepth/merge_count/main.nf Co-authored-by: Nicolas Vannieuwkerke <101190534+nvnieuwk@users.noreply.github.com> --- modules/local/exomedepth/merge_count/main.nf | 11 ++++++----- 1 file changed, 6 insertions(+), 5 deletions(-) diff --git a/modules/local/exomedepth/merge_count/main.nf b/modules/local/exomedepth/merge_count/main.nf index a7e0bec..c1fd288 100644 --- a/modules/local/exomedepth/merge_count/main.nf +++ b/modules/local/exomedepth/merge_count/main.nf @@ -6,16 +6,17 @@ process EXOMEDEPTH_COUNT_MERGE { tuple val(meta), path(files) output: - tuple val(meta), path("${meta.id}_${meta.chr}.txt") + tuple val(meta), path("${prefix}.txt") script: + prefix = task.ext.prefix ?: "${meta.id}_${meta.chr}" """ for file in $files; do - if [ -f ${meta.id}_${meta.chr}.txt ]; then - paste ${meta.id}_${meta.chr}.txt <(awk '{print \$5}' \$file) > temp_auto.txt - mv temp_auto.txt ${meta.id}_${meta.chr}.txt + if [ -f ${prefix}.txt ]; then + paste ${prefix}.txt <(awk '{print \$5}' \$file) > temp_auto.txt + mv temp_auto.txt ${prefix}.txt else - cp \$file ${meta.id}_${meta.chr}.txt + cp \$file ${prefix}.txt fi done """ From ffc19c73e555a668f1310c410c9f8cb064e0c392 Mon Sep 17 00:00:00 2001 From: BertGalle <129084478+BertGalle@users.noreply.github.com> Date: Tue, 23 Apr 2024 15:42:59 +0200 Subject: [PATCH 34/64] Update workflows/exomecnv.nf Co-authored-by: Nicolas Vannieuwkerke <101190534+nvnieuwk@users.noreply.github.com> --- workflows/exomecnv.nf | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/workflows/exomecnv.nf b/workflows/exomecnv.nf index d39b913..9cc5276 100644 --- a/workflows/exomecnv.nf +++ b/workflows/exomecnv.nf @@ -88,7 +88,7 @@ workflow EXOMECNV { .groupTuple() COUNT_MERGE_AUTO ( - grouped_counts_auto + grouped_counts_auto ) COUNT_X ( From 2ed80b87e17567077c879177df232cad77ac9c88 Mon Sep 17 00:00:00 2001 From: BertGalle Date: Fri, 26 Apr 2024 13:42:35 +0200 Subject: [PATCH 35/64] Added ExomeDepth CNV call --- bin/CNV_ExomeDepth_call.R | 144 ++++++++++++++++++ modules/local/exomedepth/cnv_call/main.nf | 37 +++++ .../local/exomedepth_cnv_call/main.nf | 20 +++ workflows/exomecnv.nf | 49 ++++-- 4 files changed, 241 insertions(+), 9 deletions(-) create mode 100755 bin/CNV_ExomeDepth_call.R create mode 100644 modules/local/exomedepth/cnv_call/main.nf create mode 100644 subworkflows/local/exomedepth_cnv_call/main.nf diff --git a/bin/CNV_ExomeDepth_call.R b/bin/CNV_ExomeDepth_call.R new file mode 100755 index 0000000..8de72a9 --- /dev/null +++ b/bin/CNV_ExomeDepth_call.R @@ -0,0 +1,144 @@ +#!/usr/bin/env Rscript + +############################################################################################ +### ExomeDepth - CNV calling ### +############################################################################################ + +### load libs ### +library("methods") # to load the function "as" +library("ExomeDepth") +ExomeDepthVersion <- packageDescription("ExomeDepth")$Version +print(paste0("...loaded ExomeDepth version: ", ExomeDepthVersion)) + +args = commandArgs(trailingOnly = TRUE) +print_usage <- function() { + cat("Usage: CNV_ExomeDepth_call.R \n") +} +if (length(commandArgs(trailingOnly = TRUE)) != 6) { + print_usage() + q("no", status = 1) # Terminate the script with an error status +} + +cnv_call_header = c("start.p", "end.p", "type", "nexons", "start", "end", "chromosome", "id", "BF", "reads.expected", "reads.observed", "reads.ratio", "exons") +sampleName <- unlist(args[1]) #sample id +countfile <- unlist(args[2]) #count matrix +exon_target <- unlist(args[3]) #exon target bed file +prefix <- unlist(args[4]) #prefix for output name +sampleNames <- unlist(strsplit(args[5] , ",")) +families <- unlist(strsplit(args[6] , ",")) #familie ids for samples + +cat("samplename: ", sampleName, "\n") +cat("count file: ", countfile, "\n") +cat("exon target file: ", exon_target, "\n") +cat("prefix: ", prefix, "\n") +cat("sample ids pool: ", sampleNames, "\n") +cat("family ids for samples: ", families, "\n") + +### load exon data ### +exons <- read.table(file=exon_target,sep="\t",header = F) +colnames(exons) <- c("chromosome","start","end","name") +# head(exons) + +### load all exon info #### +exons.GRanges <- GenomicRanges::GRanges(seqnames = exons$chromosome, + IRanges::IRanges(start=exons$start,end=exons$end), + names = exons$name + ) + +# ### read counts ### +cat("\nRead counting matrix\n") +ExomeCount.dafr <- read.table(file=countfile,sep="\t",header = TRUE) +# print(head(ExomeCount.dafr)) +# colnames(ExomeCount.dafr) + +### Call CNVs ### +## build the most appropiate reference set: aggregated reference set +## key idea: build optimized set of exomens that are well correlated with that exome + +## prepare the main matrix of read count data +countmat = as.matrix(ExomeCount.dafr[,5:dim(ExomeCount.dafr)[2]]) # remove cols 1-4 metadata +nsamples <- ncol(countmat) +# print(head(countmat)) + +## check if test sample is part of count matrix +if (is.element(sampleName, colnames(countmat))){ + print(paste(sampleName, "is part of the count matrix")) +} else{ + stop(paste("An error occurred:", sampleName, "is NOT part of the count matrix\n\n")) +} + +for (i in 1:nsamples) { + + ## select sample-id + sample = colnames(countmat)[i] + + # print(paste(sample,i,families[sample_index])) + # print(sampleNames[families != families[sample_index]]) + + ## only CNV calling for input test sample + if (sample == sampleName) { + + sample_index <- which(sampleNames == sample) + + ## perform CNV calling for the test sample + cat("\n*** CNV calling for",sample,"***\n") + + ## build reference set (pool = all other samples of run) + cat("\nSelecting reference samples from pool:\n") + print(unlist(sampleNames[families != families[sample_index]])) + cat("\n") + reference_list = select.reference.set( + test.counts = countmat[,i], + reference.count = countmat[,sampleNames[families != families[sample_index]]], + bin.length=(ExomeCount.dafr$end-ExomeCount.dafr$start)/1000, + n.bins.reduced = 10000) + cat("\nOptimized reference set: ", reference_list[[1]], "\n") + + ## construct the reference set (all other samples of run) + reference_set = apply( + X = as.matrix(ExomeCount.dafr[, reference_list$reference.choice, drop = FALSE]), #drop = FALSE: in case the reference set contains a singlle sample, it will makes sure that the subsetted object is a data frame, not a numeric vector + MAR=1, FUN=sum) + + ## CNV calling - beta-binomial model applied to the full set of exons - longest step + cat("\nCreating the ExomeDepth object\n") + all_exons = new('ExomeDepth', + test=countmat[,i], + reference=reference_set, + formula = 'cbind(test,reference) ~ 1') + ## call CNV by running the underlying hidden Markov model + ## the correlation between reference and test set should be >0.97 + ## if not: consider the output as less reliable + cat("\nCall CNVs\n") + all_exons = CallCNVs(x = all_exons, + transition.probability=10^-4, + chromosome=ExomeCount.dafr$chromosome, + start=ExomeCount.dafr$start, + end=ExomeCount.dafr$end, + name=ExomeCount.dafr$exon) + if (all(dim(all_exons@CNV.calls) != c(0,0))) {#if CNVs found + ## annotation CNV calls with exon info + cat("\nAnnotate with exon info\n") + all_exons = AnnotateExtra(x = all_exons, + reference.annotation = exons.GRanges, + min.overlap = 0.0001, + column.name= 'exons') + ## output file + ## ranking the CNV calls by confidence level + ## the BF column = Bayes factor = quantifies the statistical support for each CNV (=log10 of the likelyhood ratio of data for the CNV call divided by the null (normal copy number). + ## The higher BF, the more confident about the presence of a CNV; this is especially true for obvious large calls; for short exons: BF are bound to be unconvincing + output.cnv.file = paste(sample,"_CNVs_ExomeDepth_",prefix,".txt",sep='') + cat("\nOutput file:",output.cnv.file ,"\n") + write.table(all_exons@CNV.calls[order ( all_exons@CNV.calls$BF, decreasing = TRUE),], file=output.cnv.file, + sep='\t', row.names=FALSE, col.names=TRUE, quote=FALSE) + } + else {#no CNVs found + output.cnv.file = paste(sample,"_CNVs_ExomeDepth_",prefix,".txt",sep='') + cat("\nNo", prefix, "CNVs found\n") + cat("\nOutput file:",output.cnv.file ,"\n") + note = paste( cnv_call_header , collapse = "\t") + write(note, file=output.cnv.file) + } + cat("\nDone\n") + } +} +cat("\n\n---Finished---\n") diff --git a/modules/local/exomedepth/cnv_call/main.nf b/modules/local/exomedepth/cnv_call/main.nf new file mode 100644 index 0000000..4c44169 --- /dev/null +++ b/modules/local/exomedepth/cnv_call/main.nf @@ -0,0 +1,37 @@ +process CNV_CALL { + tag "$sample $meta2.chr" + + container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? + 'https://depot.galaxyproject.org/singularity/r-exomedepth:1.1.16--r43hfb3cda0_3' : + 'biocontainers/r-exomedepth:1.1.16--r43hfb3cda0_3' }" + + publishDir "$params.outdir/exomedepth/cnv_call", mode: 'copy' + + input: + tuple val(meta), path(exon_target) // meta.id=chrx/autosomal + tuple val(meta2), val(sample), path(countfile) // meta:id, chr, sam, fam, sample + + output: + tuple val(meta2), path("*.txt"), emit: cnvcall + path "versions.yml", emit:versions + + script: + + def VERSION = '1.1.16' + + """ + CNV_ExomeDepth_call.R \\ + $sample \\ + $countfile \\ + $exon_target \\ + $meta2.chr \\ + ${meta2.sam.join(',')} \\ + ${meta2.fam.join(',')} \\ + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + ExomeDepth: ${VERSION} + R: \$(Rscript --version | sed 's/R scripting front-end //g') + END_VERSIONS + """ +} diff --git a/subworkflows/local/exomedepth_cnv_call/main.nf b/subworkflows/local/exomedepth_cnv_call/main.nf new file mode 100644 index 0000000..1296764 --- /dev/null +++ b/subworkflows/local/exomedepth_cnv_call/main.nf @@ -0,0 +1,20 @@ + +include { CNV_CALL } from '../../../modules/local/exomedepth/cnv_call/main' + +workflow EXOMEDEPTH_CNV { + + take: + exon_target // channel: [mandatory] [ val(meta), path(bed)] + cnv_ch // channel: [mandatory] [ val(meta2), val(sample), val(countfile)] + + main: + ch_versions= Channel.empty() + + CNV_CALL(exon_target, cnv_ch) + ch_versions = ch_versions.mix(CNV_CALL.out.versions) + + emit: + cnv = CNV_CALL.out.cnvcall // channel: [ val(meta), [ txt ] ] + + versions = ch_versions +} diff --git a/workflows/exomecnv.nf b/workflows/exomecnv.nf index 9cc5276..e3580f7 100644 --- a/workflows/exomecnv.nf +++ b/workflows/exomecnv.nf @@ -16,7 +16,8 @@ include { EXOMEDEPTH_COUNT as COUNT_X } from '../subworkflows/loc include { EXOMEDEPTH_COUNT as COUNT_AUTO } from '../subworkflows/local/exomedepth_count/main' include { EXOMEDEPTH_COUNT_MERGE as COUNT_MERGE_AUTO } from '../modules/local/exomedepth/merge_count/main' include { EXOMEDEPTH_COUNT_MERGE as COUNT_MERGE_X } from '../modules/local/exomedepth/merge_count/main' - +include { EXOMEDEPTH_CNV as CNV_CALL_AUTO } from '../subworkflows/local/exomedepth_cnv_call/main' +include { EXOMEDEPTH_CNV as CNV_CALL_X } from '../subworkflows/local/exomedepth_cnv_call/main' // include { BAM_VARIANT_CALLING_EXOMEDEPTH } from '../subworkflows/local/bam_variant_calling_exomedepth/main' /* @@ -31,7 +32,6 @@ workflow EXOMECNV { ch_samplesheet // channel: samplesheet read in from --input main: - ch_versions = Channel.empty() ch_multiqc_files = Channel.empty() @@ -75,36 +75,67 @@ workflow EXOMECNV { .mix(ch_cram_prepare) .set{ ch_input_bam } - // ch_input_bam.view() + //ch_input_bam.view() COUNT_AUTO ( ch_input_bam, ch_roi_auto ) + ch_versions = ch_versions.mix(COUNT_AUTO.out.versions) grouped_counts_auto = COUNT_AUTO.out.count .map { meta, txt -> - def new_meta = [id:meta.pool,chr:"autosomal"] + def new_meta = [id:meta.pool] + [new_meta, meta.sample, meta.family, txt] + } + .groupTuple() + .map { meta, samples, families, txt -> + def new_meta = [id:meta.id, chr:"autosomal", sam:samples, fam: families] [new_meta, txt] - } - .groupTuple() + } COUNT_MERGE_AUTO ( grouped_counts_auto ) + cnv_auto_ch = COUNT_MERGE_AUTO.out + .map { meta, txt -> + [meta, meta.sam, txt] + } + .transpose(by:1) + + CNV_CALL_AUTO( + ch_roi_auto, cnv_auto_ch + ) + ch_versions = ch_versions.mix(CNV_CALL_AUTO.out.versions) + COUNT_X ( ch_input_bam, ch_roi_x ) grouped_counts_X = COUNT_X.out.count .map { meta, txt -> - def new_meta = [id:meta.pool,chr:"chrX"] + def new_meta = [id:meta.pool] + [new_meta, meta.sample, meta.family, txt] + } + .groupTuple() + .map { meta, samples, families, txt -> + def new_meta = [id:meta.id, chr:"chrX", sam:samples, fam: families] [new_meta, txt] - } - .groupTuple() + } COUNT_MERGE_X ( grouped_counts_X ) + + cnv_chrx_ch = COUNT_MERGE_X.out + .map { meta, txt -> + [meta, meta.sam, txt] + } + .transpose(by:1) + + CNV_CALL_X( + ch_roi_auto, cnv_chrx_ch + ) + // // Collate and save software versions // From b0ce66a3d8b093a3bc3f9cadda5a63571cadf432 Mon Sep 17 00:00:00 2001 From: BertGalle Date: Fri, 26 Apr 2024 13:59:39 +0200 Subject: [PATCH 36/64] removed CNV_ExomeDepth_call.R trailing whitespaces --- bin/CNV_ExomeDepth_call.R | 30 +++++++++++++++--------------- 1 file changed, 15 insertions(+), 15 deletions(-) diff --git a/bin/CNV_ExomeDepth_call.R b/bin/CNV_ExomeDepth_call.R index 8de72a9..216bdb0 100755 --- a/bin/CNV_ExomeDepth_call.R +++ b/bin/CNV_ExomeDepth_call.R @@ -53,7 +53,7 @@ ExomeCount.dafr <- read.table(file=countfile,sep="\t",header = TRUE) ### Call CNVs ### ## build the most appropiate reference set: aggregated reference set -## key idea: build optimized set of exomens that are well correlated with that exome +## key idea: build optimized set of exomens that are well correlated with that exome ## prepare the main matrix of read count data countmat = as.matrix(ExomeCount.dafr[,5:dim(ExomeCount.dafr)[2]]) # remove cols 1-4 metadata @@ -68,21 +68,21 @@ if (is.element(sampleName, colnames(countmat))){ } for (i in 1:nsamples) { - + ## select sample-id sample = colnames(countmat)[i] - + # print(paste(sample,i,families[sample_index])) # print(sampleNames[families != families[sample_index]]) ## only CNV calling for input test sample if (sample == sampleName) { - + sample_index <- which(sampleNames == sample) ## perform CNV calling for the test sample cat("\n*** CNV calling for",sample,"***\n") - + ## build reference set (pool = all other samples of run) cat("\nSelecting reference samples from pool:\n") print(unlist(sampleNames[families != families[sample_index]])) @@ -93,42 +93,42 @@ for (i in 1:nsamples) { bin.length=(ExomeCount.dafr$end-ExomeCount.dafr$start)/1000, n.bins.reduced = 10000) cat("\nOptimized reference set: ", reference_list[[1]], "\n") - + ## construct the reference set (all other samples of run) reference_set = apply( X = as.matrix(ExomeCount.dafr[, reference_list$reference.choice, drop = FALSE]), #drop = FALSE: in case the reference set contains a singlle sample, it will makes sure that the subsetted object is a data frame, not a numeric vector MAR=1, FUN=sum) - + ## CNV calling - beta-binomial model applied to the full set of exons - longest step cat("\nCreating the ExomeDepth object\n") - all_exons = new('ExomeDepth', - test=countmat[,i], + all_exons = new('ExomeDepth', + test=countmat[,i], reference=reference_set, formula = 'cbind(test,reference) ~ 1') ## call CNV by running the underlying hidden Markov model ## the correlation between reference and test set should be >0.97 ## if not: consider the output as less reliable cat("\nCall CNVs\n") - all_exons = CallCNVs(x = all_exons, + all_exons = CallCNVs(x = all_exons, transition.probability=10^-4, - chromosome=ExomeCount.dafr$chromosome, + chromosome=ExomeCount.dafr$chromosome, start=ExomeCount.dafr$start, - end=ExomeCount.dafr$end, + end=ExomeCount.dafr$end, name=ExomeCount.dafr$exon) if (all(dim(all_exons@CNV.calls) != c(0,0))) {#if CNVs found ## annotation CNV calls with exon info cat("\nAnnotate with exon info\n") - all_exons = AnnotateExtra(x = all_exons, + all_exons = AnnotateExtra(x = all_exons, reference.annotation = exons.GRanges, min.overlap = 0.0001, column.name= 'exons') ## output file ## ranking the CNV calls by confidence level - ## the BF column = Bayes factor = quantifies the statistical support for each CNV (=log10 of the likelyhood ratio of data for the CNV call divided by the null (normal copy number). + ## the BF column = Bayes factor = quantifies the statistical support for each CNV (=log10 of the likelyhood ratio of data for the CNV call divided by the null (normal copy number). ## The higher BF, the more confident about the presence of a CNV; this is especially true for obvious large calls; for short exons: BF are bound to be unconvincing output.cnv.file = paste(sample,"_CNVs_ExomeDepth_",prefix,".txt",sep='') cat("\nOutput file:",output.cnv.file ,"\n") - write.table(all_exons@CNV.calls[order ( all_exons@CNV.calls$BF, decreasing = TRUE),], file=output.cnv.file, + write.table(all_exons@CNV.calls[order ( all_exons@CNV.calls$BF, decreasing = TRUE),], file=output.cnv.file, sep='\t', row.names=FALSE, col.names=TRUE, quote=FALSE) } else {#no CNVs found From 43c1cd3bc4d9ab35e2603f363a265d24bf46a39e Mon Sep 17 00:00:00 2001 From: BertGalle Date: Fri, 26 Apr 2024 14:55:39 +0200 Subject: [PATCH 37/64] Removed count and cnv_call subworkflows --- modules/local/exomedepth/merge_count/main.nf | 2 +- subworkflows/local/exomedepth_count/main.nf | 1 - workflows/exomecnv.nf | 18 +++++++++--------- 3 files changed, 10 insertions(+), 11 deletions(-) diff --git a/modules/local/exomedepth/merge_count/main.nf b/modules/local/exomedepth/merge_count/main.nf index c1fd288..45363fb 100644 --- a/modules/local/exomedepth/merge_count/main.nf +++ b/modules/local/exomedepth/merge_count/main.nf @@ -1,5 +1,5 @@ // MERGE COUNT FILES -process EXOMEDEPTH_COUNT_MERGE { +process COUNT_MERGE { publishDir "$params.outdir/exomedepth/counts", mode: 'copy' input: diff --git a/subworkflows/local/exomedepth_count/main.nf b/subworkflows/local/exomedepth_count/main.nf index 991f310..29c50b8 100644 --- a/subworkflows/local/exomedepth_count/main.nf +++ b/subworkflows/local/exomedepth_count/main.nf @@ -9,7 +9,6 @@ workflow EXOMEDEPTH_COUNT { exon_target // channel: [mandatory] [ val(meta2), path(bed) ] main: - ch_versions = Channel.empty() // diff --git a/workflows/exomecnv.nf b/workflows/exomecnv.nf index e3580f7..ca4de85 100644 --- a/workflows/exomecnv.nf +++ b/workflows/exomecnv.nf @@ -11,13 +11,13 @@ include { softwareVersionsToYAML } from '../subworkflows/nf-core/utils_nfcore_pi include { methodsDescriptionText } from '../subworkflows/local/utils_nfcore_exomecnv_pipeline' // local -include { CRAM_PREPARE } from '../subworkflows/local/cram_prepare/main' -include { EXOMEDEPTH_COUNT as COUNT_X } from '../subworkflows/local/exomedepth_count/main' -include { EXOMEDEPTH_COUNT as COUNT_AUTO } from '../subworkflows/local/exomedepth_count/main' -include { EXOMEDEPTH_COUNT_MERGE as COUNT_MERGE_AUTO } from '../modules/local/exomedepth/merge_count/main' -include { EXOMEDEPTH_COUNT_MERGE as COUNT_MERGE_X } from '../modules/local/exomedepth/merge_count/main' -include { EXOMEDEPTH_CNV as CNV_CALL_AUTO } from '../subworkflows/local/exomedepth_cnv_call/main' -include { EXOMEDEPTH_CNV as CNV_CALL_X } from '../subworkflows/local/exomedepth_cnv_call/main' +include { CRAM_PREPARE } from '../subworkflows/local/cram_prepare/main' +include { COUNT as COUNT_X } from '../modules/local/exomedepth/count/main' +include { COUNT as COUNT_AUTO } from '../modules/local/exomedepth/count/main' +include { COUNT_MERGE as COUNT_MERGE_AUTO } from '../modules/local/exomedepth/merge_count/main' +include { COUNT_MERGE as COUNT_MERGE_X } from '../modules/local/exomedepth/merge_count/main' +include { CNV_CALL as CNV_CALL_AUTO } from '../modules/local/exomedepth/cnv_call/main' +include { CNV_CALL as CNV_CALL_X } from '../modules/local/exomedepth/cnv_call/main' // include { BAM_VARIANT_CALLING_EXOMEDEPTH } from '../subworkflows/local/bam_variant_calling_exomedepth/main' /* @@ -81,7 +81,7 @@ workflow EXOMECNV { ) ch_versions = ch_versions.mix(COUNT_AUTO.out.versions) - grouped_counts_auto = COUNT_AUTO.out.count + grouped_counts_auto = COUNT_AUTO.out.counts .map { meta, txt -> def new_meta = [id:meta.pool] [new_meta, meta.sample, meta.family, txt] @@ -111,7 +111,7 @@ workflow EXOMECNV { ch_input_bam, ch_roi_x ) - grouped_counts_X = COUNT_X.out.count + grouped_counts_X = COUNT_X.out.counts .map { meta, txt -> def new_meta = [id:meta.pool] [new_meta, meta.sample, meta.family, txt] From b9d3eddbffc593a4c91bf4c855bdd27873bd6ae4 Mon Sep 17 00:00:00 2001 From: BertGalle Date: Fri, 26 Apr 2024 15:02:59 +0200 Subject: [PATCH 38/64] Deleted workflows --- .../local/exomedepth_cnv_call/main.nf | 20 --------------- subworkflows/local/exomedepth_count/main.nf | 25 ------------------- 2 files changed, 45 deletions(-) delete mode 100644 subworkflows/local/exomedepth_cnv_call/main.nf delete mode 100644 subworkflows/local/exomedepth_count/main.nf diff --git a/subworkflows/local/exomedepth_cnv_call/main.nf b/subworkflows/local/exomedepth_cnv_call/main.nf deleted file mode 100644 index 1296764..0000000 --- a/subworkflows/local/exomedepth_cnv_call/main.nf +++ /dev/null @@ -1,20 +0,0 @@ - -include { CNV_CALL } from '../../../modules/local/exomedepth/cnv_call/main' - -workflow EXOMEDEPTH_CNV { - - take: - exon_target // channel: [mandatory] [ val(meta), path(bed)] - cnv_ch // channel: [mandatory] [ val(meta2), val(sample), val(countfile)] - - main: - ch_versions= Channel.empty() - - CNV_CALL(exon_target, cnv_ch) - ch_versions = ch_versions.mix(CNV_CALL.out.versions) - - emit: - cnv = CNV_CALL.out.cnvcall // channel: [ val(meta), [ txt ] ] - - versions = ch_versions -} diff --git a/subworkflows/local/exomedepth_count/main.nf b/subworkflows/local/exomedepth_count/main.nf deleted file mode 100644 index 29c50b8..0000000 --- a/subworkflows/local/exomedepth_count/main.nf +++ /dev/null @@ -1,25 +0,0 @@ - -// Convert CRAM files -include { COUNT } from '../../../modules/local/exomedepth/count/main' - -workflow EXOMEDEPTH_COUNT { - - take: - ch_bam // channel: [mandatory] [ val(meta), path(bam), path(bai) ] - exon_target // channel: [mandatory] [ val(meta2), path(bed) ] - - main: - ch_versions = Channel.empty() - - // - // convert CRAM files to BAM files - // - COUNT(ch_bam, exon_target) - ch_versions = ch_versions.mix(COUNT.out.versions) - - emit: - count = COUNT.out.counts // channel: [ val(meta), [ txt ] ] - - versions = ch_versions // channel: [ versions.yml ] -} - From c944db39a1c53fd0172bace3b254297c71968b08 Mon Sep 17 00:00:00 2001 From: BertGalle Date: Fri, 26 Apr 2024 16:36:24 +0200 Subject: [PATCH 39/64] test --- testfile | 1 + 1 file changed, 1 insertion(+) create mode 100644 testfile diff --git a/testfile b/testfile new file mode 100644 index 0000000..f58b43e --- /dev/null +++ b/testfile @@ -0,0 +1 @@ +fsdfsq From 130b589a7042bbe818d9fafa7c7c075918f01ac4 Mon Sep 17 00:00:00 2001 From: BertGalle Date: Fri, 26 Apr 2024 16:37:32 +0200 Subject: [PATCH 40/64] test --- testfile | 1 - 1 file changed, 1 deletion(-) delete mode 100644 testfile diff --git a/testfile b/testfile deleted file mode 100644 index f58b43e..0000000 --- a/testfile +++ /dev/null @@ -1 +0,0 @@ -fsdfsq From 8a63d505eb686f80d9fcfeffc1461338043683a1 Mon Sep 17 00:00:00 2001 From: BertGalle Date: Tue, 30 Apr 2024 16:56:02 +0200 Subject: [PATCH 41/64] Grouped CNV files per sample and converted to VCF --- assets/exomedepth.yaml | 54 +++++++++++++ conf/modules.config | 4 + conf/test.config | 2 + modules.json | 5 ++ modules/local/exomedepth/cnv_call/main.nf | 3 +- modules/local/exomedepth/merge_cnv/main.nf | 20 +++++ modules/local/exomedepth/merge_count/main.nf | 2 + modules/nf-core/bedgovcf/environment.yml | 7 ++ modules/nf-core/bedgovcf/main.nf | 49 ++++++++++++ modules/nf-core/bedgovcf/meta.yml | 57 ++++++++++++++ modules/nf-core/bedgovcf/tests/main.nf.test | 77 +++++++++++++++++++ .../nf-core/bedgovcf/tests/main.nf.test.snap | 60 +++++++++++++++ modules/nf-core/bedgovcf/tests/tags.yml | 2 + workflows/exomecnv.nf | 60 +++++++++++---- 14 files changed, 384 insertions(+), 18 deletions(-) create mode 100644 assets/exomedepth.yaml create mode 100644 modules/local/exomedepth/merge_cnv/main.nf create mode 100644 modules/nf-core/bedgovcf/environment.yml create mode 100644 modules/nf-core/bedgovcf/main.nf create mode 100644 modules/nf-core/bedgovcf/meta.yml create mode 100644 modules/nf-core/bedgovcf/tests/main.nf.test create mode 100644 modules/nf-core/bedgovcf/tests/main.nf.test.snap create mode 100644 modules/nf-core/bedgovcf/tests/tags.yml diff --git a/assets/exomedepth.yaml b/assets/exomedepth.yaml new file mode 100644 index 0000000..48e4d31 --- /dev/null +++ b/assets/exomedepth.yaml @@ -0,0 +1,54 @@ +# Use --skip 1 with this config +chrom: + value: $6 + +pos: + value: $4 + +id: + value: $7 + +ref: + value: "." + +alt: + value: ~if $2 == deletion + +filter: + value: "." + +info: + - name: SVTYPE + value: ~if $2 == deletion DEL DUP + number: 1 + type: string + description: Type of structural variant + - name: END + value: $5 + type: integer + description: End position of structural variant + - name: BF + value: $8 + type: float + description: Bayes factor + - name: NEXONS + value: $3 + type: integer + description: Number of exons + - name: READSEXP + value: $9 + type: integer + description: Expected reads + - name: READSOBS + value: $10 + type: integer + description: Observed reads + - name: READRATIO + value: $11 + type: float + description: Ratio observed/expected reads + - name: EXONSENS + value: $12 + type: string + description: Exons + diff --git a/conf/modules.config b/conf/modules.config index 800f8fc..62a2160 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -46,4 +46,8 @@ process { saveAs: { filename -> filename.equals('versions.yml') ? null : filename } ] } + + withName: 'BEDGOVCF' { + ext.args = { "--skip 1" } + } } diff --git a/conf/test.config b/conf/test.config index 0da3a97..ce7c8a2 100644 --- a/conf/test.config +++ b/conf/test.config @@ -31,6 +31,8 @@ params { roi_auto = "https://raw.githubusercontent.com/nf-cmgg/test-datasets/exomecnv/data/genomics/homo_sapiens/genome/roi/Homo_sapiens.GRCh38.105.chr21_protein_coding_basic_sorted_merged_autosomal.bed" roi_chrx = "https://raw.githubusercontent.com/nf-cmgg/test-datasets/exomecnv/data/genomics/homo_sapiens/genome/roi/Homo_sapiens.GRCh38.105.chrX_protein_coding_basic_sorted_merged.bed" + yamlconfig = "${projectDir}/assets/exomedepth.yaml" + exomedepth = false } diff --git a/modules.json b/modules.json index 0dbc9a9..275dfa5 100644 --- a/modules.json +++ b/modules.json @@ -5,6 +5,11 @@ "https://github.com/nf-core/modules.git": { "modules": { "nf-core": { + "bedgovcf": { + "branch": "master", + "git_sha": "338a5572efbda56110cea2f657615920d8dce58c", + "installed_by": ["modules"] + }, "multiqc": { "branch": "master", "git_sha": "b7ebe95761cd389603f9cc0e0dc384c0f663815a", diff --git a/modules/local/exomedepth/cnv_call/main.nf b/modules/local/exomedepth/cnv_call/main.nf index 4c44169..cdb512f 100644 --- a/modules/local/exomedepth/cnv_call/main.nf +++ b/modules/local/exomedepth/cnv_call/main.nf @@ -1,5 +1,6 @@ process CNV_CALL { tag "$sample $meta2.chr" + label 'process_low' container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? 'https://depot.galaxyproject.org/singularity/r-exomedepth:1.1.16--r43hfb3cda0_3' : @@ -12,7 +13,7 @@ process CNV_CALL { tuple val(meta2), val(sample), path(countfile) // meta:id, chr, sam, fam, sample output: - tuple val(meta2), path("*.txt"), emit: cnvcall + tuple val(meta2), val (sample), path("*.txt"), emit: cnvcall path "versions.yml", emit:versions script: diff --git a/modules/local/exomedepth/merge_cnv/main.nf b/modules/local/exomedepth/merge_cnv/main.nf new file mode 100644 index 0000000..3ff104d --- /dev/null +++ b/modules/local/exomedepth/merge_cnv/main.nf @@ -0,0 +1,20 @@ +//MERGE CNV CALL FILES +process CNV_MERGE { + tag "$meta" + + publishDir "$params.outdir/exomedepth/cnv_call", mode: 'copy' + + input: + tuple val(meta), path(auto), path(chrx) + + output: + tuple val(meta), path("${prefix}.txt") + + script: + prefix = task.ext.prefix ?: "${meta}_CNVs_ExomeDepth" + """ + cp $auto "${prefix}.txt" + tail +2 $chrx >> "${prefix}.txt" + """ + +} diff --git a/modules/local/exomedepth/merge_count/main.nf b/modules/local/exomedepth/merge_count/main.nf index 45363fb..ae2e255 100644 --- a/modules/local/exomedepth/merge_count/main.nf +++ b/modules/local/exomedepth/merge_count/main.nf @@ -1,5 +1,7 @@ // MERGE COUNT FILES process COUNT_MERGE { + tag "$meta.id $meta.chr" + publishDir "$params.outdir/exomedepth/counts", mode: 'copy' input: diff --git a/modules/nf-core/bedgovcf/environment.yml b/modules/nf-core/bedgovcf/environment.yml new file mode 100644 index 0000000..61cfbcc --- /dev/null +++ b/modules/nf-core/bedgovcf/environment.yml @@ -0,0 +1,7 @@ +name: bedgovcf +channels: + - conda-forge + - bioconda + - defaults +dependencies: + - bioconda::bedgovcf=0.1.0 diff --git a/modules/nf-core/bedgovcf/main.nf b/modules/nf-core/bedgovcf/main.nf new file mode 100644 index 0000000..f39f404 --- /dev/null +++ b/modules/nf-core/bedgovcf/main.nf @@ -0,0 +1,49 @@ +process BEDGOVCF { + tag "$meta.id" + label 'process_low' + + conda "${moduleDir}/environment.yml" + container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? + 'https://depot.galaxyproject.org/singularity/bedgovcf:0.1.0--h9ee0642_0': + 'biocontainers/bedgovcf:0.1.0--h9ee0642_0' }" + + input: + tuple val(meta), path(bed), path(config) + tuple val(meta2), path(fai) + + output: + tuple val(meta), path("*.vcf.gz"), emit: vcf + path "versions.yml" , emit: versions + + when: + task.ext.when == null || task.ext.when + + script: + def args = task.ext.args ?: '' + def args2 = task.ext.args2 ?: '' + def prefix = task.ext.prefix ?: "${meta.id}" + """ + bedgovcf \\ + $args \\ + --bed $bed \\ + --fai $fai \\ + --config $config \\ + | bgzip --stdout --threads $task.cpus $args2 > ${prefix}.vcf.gz + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + bedgovcf: \$(echo \$(bedgovcf --version 2>&1) | sed 's/^bedgovcf version //' ) + END_VERSIONS + """ + + stub: + def prefix = task.ext.prefix ?: "${meta.id}" + """ + touch ${prefix}.vcf.gz + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + bedgovcf: \$(echo \$(bedgovcf --version 2>&1) | sed 's/^bedgovcf version //' ) + END_VERSIONS + """ +} diff --git a/modules/nf-core/bedgovcf/meta.yml b/modules/nf-core/bedgovcf/meta.yml new file mode 100644 index 0000000..406935e --- /dev/null +++ b/modules/nf-core/bedgovcf/meta.yml @@ -0,0 +1,57 @@ +--- +name: "bedgovcf" +description: Convert a BED file to a VCF file according to a YAML config +keywords: + - bed + - vcf + - conversion + - variants +tools: + - "bedgovcf": + description: "A simple tool to convert BED files to VCF files" + homepage: "https://github.com/nvnieuwk/bedgovcf" + documentation: "https://github.com/nvnieuwk/bedgovcf" + tool_dev_url: "https://github.com/nvnieuwk/bedgovcf" + licence: ["MIT"] + +input: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. `[ id:'test', single_end:false ]` + - bed: + type: file + description: The BED file to convert to VCF + pattern: "*.bed" + - config: + type: file + description: The config file to use for the conversion + pattern: "*.{yml,yaml}" + - meta2: + type: map + description: | + Groovy Map containing fasta index information + e.g. `[ id:'test', single_end:false ]` + - fai: + type: file + description: The fasta index file + pattern: "*.fai" + +output: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. `[ id:'test', single_end:false ]` + - versions: + type: file + description: File containing software versions + pattern: "versions.yml" + - vcf: + type: file + description: The converted VCF file + pattern: "*.vcf.gz" + +authors: + - "@nvnieuwk" diff --git a/modules/nf-core/bedgovcf/tests/main.nf.test b/modules/nf-core/bedgovcf/tests/main.nf.test new file mode 100644 index 0000000..f55fbb6 --- /dev/null +++ b/modules/nf-core/bedgovcf/tests/main.nf.test @@ -0,0 +1,77 @@ +nextflow_process { + + name "Test Process BEDGOVCF" + script "../main.nf" + process "BEDGOVCF" + tag "modules" + tag "modules_nfcore" + tag "bedgovcf" + + test("homo_sapiens - [bed, config] - fai") { + + // WARNING: md5sum between local tests and GHA tests differ + // Please change them manually after every snapshot update + when { + params { + outdir = $outputDir + } + process { + """ + raw_ch = Channel.of([ + [ id:'test' ], // meta map + file(params.test_data['homo_sapiens']['genome']['genome_bed'], checkIfExists: true) + ]) + config = Channel.of(["config.yaml", "header:", " - name: test", " content: test"]) + .collectFile(newLine:true, sort:'index') + input[0] = raw_ch.combine(config) + input[1] = [ + [id:"ref"], + file(params.test_data['homo_sapiens']['genome']['genome_fasta_fai'], checkIfExists: true) + ] + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(process.out).match() } + + ) + } + } + + + test("homo_sapiens - [bed, config] - fai STUB") { + options "-stub" + when { + params { + outdir = $outputDir + } + process { + """ + raw_ch = Channel.of([ + [ id:'test' ], // meta map + file(params.test_data['homo_sapiens']['genome']['genome_bed'], checkIfExists: true) + ]) + config = Channel.of(["config.yaml", "header:", " - name: test", " content: test"]) + .collectFile(newLine:true, sort:'index') + input[0] = raw_ch.combine(config) + input[1] = [ + [id:"ref"], + file(params.test_data['homo_sapiens']['genome']['genome_fasta_fai'], checkIfExists: true) + ] + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(process.out).match() } + + ) + } + } + +} diff --git a/modules/nf-core/bedgovcf/tests/main.nf.test.snap b/modules/nf-core/bedgovcf/tests/main.nf.test.snap new file mode 100644 index 0000000..2e34fe4 --- /dev/null +++ b/modules/nf-core/bedgovcf/tests/main.nf.test.snap @@ -0,0 +1,60 @@ +{ + "homo_sapiens - [bed, config] - fai STUB": { + "content": [ + { + "0": [ + [ + { + "id": "test" + }, + "test.vcf.gz:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "1": [ + "versions.yml:md5,27e259763ddc44796207f766afb94887" + ], + "vcf": [ + [ + { + "id": "test" + }, + "test.vcf.gz:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "versions": [ + "versions.yml:md5,27e259763ddc44796207f766afb94887" + ] + } + ], + "timestamp": "2023-11-14T12:45:41.931292108" + }, + "homo_sapiens - [bed, config] - fai": { + "content": [ + { + "0": [ + [ + { + "id": "test" + }, + "test.vcf.gz:md5,a9cb689f02723f7e0e4d674ef144b48c" + ] + ], + "1": [ + "versions.yml:md5,27e259763ddc44796207f766afb94887" + ], + "vcf": [ + [ + { + "id": "test" + }, + "test.vcf.gz:md5,a9cb689f02723f7e0e4d674ef144b48c" + ] + ], + "versions": [ + "versions.yml:md5,27e259763ddc44796207f766afb94887" + ] + } + ], + "timestamp": "2023-11-14T12:45:36.218283171" + } +} \ No newline at end of file diff --git a/modules/nf-core/bedgovcf/tests/tags.yml b/modules/nf-core/bedgovcf/tests/tags.yml new file mode 100644 index 0000000..d66b105 --- /dev/null +++ b/modules/nf-core/bedgovcf/tests/tags.yml @@ -0,0 +1,2 @@ +bedgovcf: + - modules/nf-core/bedgovcf/** diff --git a/workflows/exomecnv.nf b/workflows/exomecnv.nf index ca4de85..79f72e4 100644 --- a/workflows/exomecnv.nf +++ b/workflows/exomecnv.nf @@ -18,6 +18,8 @@ include { COUNT_MERGE as COUNT_MERGE_AUTO } from '../modules/local/exomedepth/ include { COUNT_MERGE as COUNT_MERGE_X } from '../modules/local/exomedepth/merge_count/main' include { CNV_CALL as CNV_CALL_AUTO } from '../modules/local/exomedepth/cnv_call/main' include { CNV_CALL as CNV_CALL_X } from '../modules/local/exomedepth/cnv_call/main' +include { CNV_MERGE } from '../modules/local/exomedepth/merge_cnv/main' +include { BEDGOVCF } from '../modules/nf-core/bedgovcf/main' // include { BAM_VARIANT_CALLING_EXOMEDEPTH } from '../subworkflows/local/bam_variant_calling_exomedepth/main' /* @@ -35,38 +37,26 @@ workflow EXOMECNV { ch_versions = Channel.empty() ch_multiqc_files = Channel.empty() - // // Importing and convert the input files passed through the parameters to channels - // + // Branch into CRAM and BAM files - // branch into CRAM and BAM files ch_samplesheet.branch { meta, cram, crai -> CRAM: cram.extension == "cram" BAM: cram.extension == "bam" } .set{ ch_input_prepare } - - //ch_input_prepare.BAM.view { "BAM: $it" } - //ch_input_prepare.CRAM.view { "CRAM: $it" } - - ch_fasta = Channel.fromPath(params.fasta).map{ [[id:"reference"], it]}.collect() ch_fai = params.fai ? Channel.fromPath(params.fai).map{ [[id:"reference"], it]}.collect() : null ch_roi_auto = Channel.fromPath(params.roi_auto).map{ [[id:"autosomal"], it]}.collect() ch_roi_x = Channel.fromPath(params.roi_chrx).map{ [[id:"chrX"], it]}.collect() - // ch_fasta.view() - // ch_fai.view() - // ch_roi_auto.view() - // ch_roi_auto.view() - // SUBWORKFLOW: Convert CRAM to BAM if no BAM file was provided + CRAM_PREPARE ( ch_input_prepare.CRAM, ch_fasta, ch_fai ) - // ch_input_prepare.BAM.view{ "BAM: $it" } - // CRAM_PREPARE.out.bai.view{ "samtools: $it" } + CRAM_PREPARE.out.bam .join(CRAM_PREPARE.out.bai) .set{ ch_cram_prepare } @@ -75,12 +65,17 @@ workflow EXOMECNV { .mix(ch_cram_prepare) .set{ ch_input_bam } - //ch_input_bam.view() + //MODULE: Count autosomal reads per sample (count file for each sample) + + if (params.exomedepth) { + COUNT_AUTO ( ch_input_bam, ch_roi_auto ) ch_versions = ch_versions.mix(COUNT_AUTO.out.versions) + //MODULE: Group autosomal counts per pool (count file for each pool) + grouped_counts_auto = COUNT_AUTO.out.counts .map { meta, txt -> def new_meta = [id:meta.pool] @@ -96,6 +91,8 @@ workflow EXOMECNV { grouped_counts_auto ) + //MODULE: Autosomal CNV call per sample (file for each sample) + cnv_auto_ch = COUNT_MERGE_AUTO.out .map { meta, txt -> [meta, meta.sam, txt] @@ -107,10 +104,14 @@ workflow EXOMECNV { ) ch_versions = ch_versions.mix(CNV_CALL_AUTO.out.versions) + //MODULE: Count chrX reads per sample (count file for each sample) + COUNT_X ( ch_input_bam, ch_roi_x ) + //MODULE: Group chrX counts per pool (count file for each pool) + grouped_counts_X = COUNT_X.out.counts .map { meta, txt -> def new_meta = [id:meta.pool] @@ -126,6 +127,8 @@ workflow EXOMECNV { grouped_counts_X ) + //MODULE: ChrX CNV call per sample (file for each sample) + cnv_chrx_ch = COUNT_MERGE_X.out .map { meta, txt -> [meta, meta.sam, txt] @@ -136,7 +139,30 @@ workflow EXOMECNV { ch_roi_auto, cnv_chrx_ch ) - // + //MODULE: Group autosomal and chrX CNV per sample (one file for each sample) + + cnv_merge_ch = CNV_CALL_AUTO.out.cnvcall + .combine(CNV_CALL_X.out.cnvcall, by:1) + .map{ sample, meta, auto, meta2, x -> + [sample, auto, x] + } + + CNV_MERGE(cnv_merge_ch) + + //MODULE: Convert file to VCF according to a YAML config + + bedgovcf_input = CNV_MERGE.out + .map{ meta, bed -> + def new_meta = [id:meta] + [new_meta, bed, params.yamlconfig] + } + + BEDGOVCF(bedgovcf_input, ch_fai) + ch_versions = ch_versions.mix(BEDGOVCF.out.versions) + + } + + // Collate and save software versions // softwareVersionsToYAML(ch_versions) From b417a5ab100ba4d981ab4ae6134ea0246341548c Mon Sep 17 00:00:00 2001 From: BertGalle Date: Tue, 7 May 2024 11:19:57 +0200 Subject: [PATCH 42/64] ExomeDepth in subworkflow + new EnsemblVEP subworkflow --- conf/modules.config | 15 ++ conf/test.config | 11 +- modules.json | 11 +- modules/nf-core/bedgovcf/environment.yml | 2 +- modules/nf-core/bedgovcf/main.nf | 6 +- .../nf-core/bedgovcf/tests/main.nf.test.snap | 20 ++- .../nf-core/ensemblvep/vep/environment.yml | 7 + modules/nf-core/ensemblvep/vep/main.nf | 70 ++++++++ modules/nf-core/ensemblvep/vep/meta.yml | 92 ++++++++++ .../nf-core/ensemblvep/vep/tests/main.nf.test | 114 ++++++++++++ .../ensemblvep/vep/tests/main.nf.test.snap | 26 +++ .../ensemblvep/vep/tests/nextflow.config | 12 ++ .../ensemblvep/vep/tests/tab.gz.config | 5 + modules/nf-core/ensemblvep/vep/tests/tags.yml | 2 + .../nf-core/ensemblvep/vep/tests/vcf.config | 5 + modules/nf-core/samtools/convert/main.nf | 15 ++ .../samtools/convert/tests/main.nf.test | 32 ++++ .../samtools/convert/tests/main.nf.test.snap | 65 +++++++ nextflow.config | 9 + nextflow_schema.json | 50 +++++- .../bam_variant_calling_exomedepth/main.nf | 37 ---- subworkflows/local/cram_prepare/main.nf | 28 --- subworkflows/local/exomedepth/main.nf | 157 +++++++++++++++++ subworkflows/local/vcf_annotation/main.nf | 39 +++++ .../nf-core/utils_nfcore_pipeline/main.nf | 8 +- workflows/exomecnv.nf | 164 ++++-------------- 26 files changed, 789 insertions(+), 213 deletions(-) create mode 100644 modules/nf-core/ensemblvep/vep/environment.yml create mode 100644 modules/nf-core/ensemblvep/vep/main.nf create mode 100644 modules/nf-core/ensemblvep/vep/meta.yml create mode 100644 modules/nf-core/ensemblvep/vep/tests/main.nf.test create mode 100644 modules/nf-core/ensemblvep/vep/tests/main.nf.test.snap create mode 100644 modules/nf-core/ensemblvep/vep/tests/nextflow.config create mode 100644 modules/nf-core/ensemblvep/vep/tests/tab.gz.config create mode 100644 modules/nf-core/ensemblvep/vep/tests/tags.yml create mode 100644 modules/nf-core/ensemblvep/vep/tests/vcf.config delete mode 100644 subworkflows/local/bam_variant_calling_exomedepth/main.nf delete mode 100644 subworkflows/local/cram_prepare/main.nf create mode 100644 subworkflows/local/exomedepth/main.nf create mode 100644 subworkflows/local/vcf_annotation/main.nf diff --git a/conf/modules.config b/conf/modules.config index 62a2160..8e02374 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -50,4 +50,19 @@ process { withName: 'BEDGOVCF' { ext.args = { "--skip 1" } } + + withName: 'ENSEMBLVEP_VEP' { + ext.prefix = {"${meta.id}.vep"} + ext.args = {[ + '--vcf', + '--format vcf', + '--coding_only', + '--offline', + '--no_stats', + '--per_gene', + '--merged', + '--exclude_predicted', + '--fields "SYMBOL,Gene"', + ].join(' ').trim()} + } } diff --git a/conf/test.config b/conf/test.config index ce7c8a2..60100d6 100644 --- a/conf/test.config +++ b/conf/test.config @@ -24,6 +24,7 @@ params { input = "${projectDir}/assets/samplesheet.csv" // Genome references + // vep_fasta = "/home/bertg/vep/hg38.fa" fasta = 'https://raw.githubusercontent.com/nf-cmgg/test-datasets/exomecnv/data/genomics/homo_sapiens/genome/seq/GCA_000001405.15_GRCh38_full_plus_hs38d1_analysis_set_chr21_and_chrX_subset.fna' fai = 'https://raw.githubusercontent.com/nf-cmgg/test-datasets/exomecnv/data/genomics/homo_sapiens/genome/seq/GCA_000001405.15_GRCh38_full_plus_hs38d1_analysis_set_chr21_and_chrX_subset.fna.fai' @@ -33,6 +34,14 @@ params { yamlconfig = "${projectDir}/assets/exomedepth.yaml" exomedepth = false -} + // VEP options + annotate = null + vep_cache = null + vep_assembly = "GRCh38" + vep_cache_version = 105 + species = "homo_sapiens" + +} + diff --git a/modules.json b/modules.json index 275dfa5..abd3e44 100644 --- a/modules.json +++ b/modules.json @@ -7,7 +7,12 @@ "nf-core": { "bedgovcf": { "branch": "master", - "git_sha": "338a5572efbda56110cea2f657615920d8dce58c", + "git_sha": "71a96063a7e09be7ea48f069d134a56ce86c9ba6", + "installed_by": ["modules"] + }, + "ensemblvep/vep": { + "branch": "master", + "git_sha": "b42fec6f7c6e5d0716685cabb825ef6bf6e386b5", "installed_by": ["modules"] }, "multiqc": { @@ -17,7 +22,7 @@ }, "samtools/convert": { "branch": "master", - "git_sha": "1b93e06140e6807cc408519759f68bd26da63a00", + "git_sha": "b42fec6f7c6e5d0716685cabb825ef6bf6e386b5", "installed_by": ["modules"] } } @@ -31,7 +36,7 @@ }, "utils_nfcore_pipeline": { "branch": "master", - "git_sha": "5caf7640a9ef1d18d765d55339be751bb0969dfa", + "git_sha": "92de218a329bfc9a9033116eb5f65fd270e72ba3", "installed_by": ["subworkflows"] }, "utils_nfvalidation_plugin": { diff --git a/modules/nf-core/bedgovcf/environment.yml b/modules/nf-core/bedgovcf/environment.yml index 61cfbcc..f19a947 100644 --- a/modules/nf-core/bedgovcf/environment.yml +++ b/modules/nf-core/bedgovcf/environment.yml @@ -4,4 +4,4 @@ channels: - bioconda - defaults dependencies: - - bioconda::bedgovcf=0.1.0 + - bioconda::bedgovcf=0.1.1 diff --git a/modules/nf-core/bedgovcf/main.nf b/modules/nf-core/bedgovcf/main.nf index f39f404..e864159 100644 --- a/modules/nf-core/bedgovcf/main.nf +++ b/modules/nf-core/bedgovcf/main.nf @@ -4,8 +4,8 @@ process BEDGOVCF { conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/bedgovcf:0.1.0--h9ee0642_0': - 'biocontainers/bedgovcf:0.1.0--h9ee0642_0' }" + 'https://depot.galaxyproject.org/singularity/bedgovcf:0.1.1--h9ee0642_1': + 'biocontainers/bedgovcf:0.1.1--h9ee0642_1' }" input: tuple val(meta), path(bed), path(config) @@ -39,7 +39,7 @@ process BEDGOVCF { stub: def prefix = task.ext.prefix ?: "${meta.id}" """ - touch ${prefix}.vcf.gz + echo "" | gzip > ${prefix}.vcf.gz cat <<-END_VERSIONS > versions.yml "${task.process}": diff --git a/modules/nf-core/bedgovcf/tests/main.nf.test.snap b/modules/nf-core/bedgovcf/tests/main.nf.test.snap index 2e34fe4..dddabbc 100644 --- a/modules/nf-core/bedgovcf/tests/main.nf.test.snap +++ b/modules/nf-core/bedgovcf/tests/main.nf.test.snap @@ -7,7 +7,7 @@ { "id": "test" }, - "test.vcf.gz:md5,d41d8cd98f00b204e9800998ecf8427e" + "test.vcf.gz:md5,68b329da9893e34099c7d8ad5cb9c940" ] ], "1": [ @@ -18,7 +18,7 @@ { "id": "test" }, - "test.vcf.gz:md5,d41d8cd98f00b204e9800998ecf8427e" + "test.vcf.gz:md5,68b329da9893e34099c7d8ad5cb9c940" ] ], "versions": [ @@ -26,7 +26,11 @@ ] } ], - "timestamp": "2023-11-14T12:45:41.931292108" + "meta": { + "nf-test": "0.8.4", + "nextflow": "24.02.0" + }, + "timestamp": "2024-05-02T10:51:08.632383757" }, "homo_sapiens - [bed, config] - fai": { "content": [ @@ -36,7 +40,7 @@ { "id": "test" }, - "test.vcf.gz:md5,a9cb689f02723f7e0e4d674ef144b48c" + "test.vcf.gz:md5,49f3bd11396cb17cba8f97005b22384c" ] ], "1": [ @@ -47,7 +51,7 @@ { "id": "test" }, - "test.vcf.gz:md5,a9cb689f02723f7e0e4d674ef144b48c" + "test.vcf.gz:md5,49f3bd11396cb17cba8f97005b22384c" ] ], "versions": [ @@ -55,6 +59,10 @@ ] } ], - "timestamp": "2023-11-14T12:45:36.218283171" + "meta": { + "nf-test": "0.8.4", + "nextflow": "24.02.0" + }, + "timestamp": "2024-05-02T10:50:05.709267287" } } \ No newline at end of file diff --git a/modules/nf-core/ensemblvep/vep/environment.yml b/modules/nf-core/ensemblvep/vep/environment.yml new file mode 100644 index 0000000..91457c0 --- /dev/null +++ b/modules/nf-core/ensemblvep/vep/environment.yml @@ -0,0 +1,7 @@ +name: ensemblvep_vep +channels: + - conda-forge + - bioconda + - defaults +dependencies: + - bioconda::ensembl-vep=111.0 diff --git a/modules/nf-core/ensemblvep/vep/main.nf b/modules/nf-core/ensemblvep/vep/main.nf new file mode 100644 index 0000000..e82471a --- /dev/null +++ b/modules/nf-core/ensemblvep/vep/main.nf @@ -0,0 +1,70 @@ +process ENSEMBLVEP_VEP { + tag "$meta.id" + label 'process_medium' + + conda "${moduleDir}/environment.yml" + container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? + 'https://depot.galaxyproject.org/singularity/ensembl-vep:111.0--pl5321h2a3209d_0' : + 'biocontainers/ensembl-vep:111.0--pl5321h2a3209d_0' }" + + input: + tuple val(meta), path(vcf), path(custom_extra_files) + val genome + val species + val cache_version + path cache + tuple val(meta2), path(fasta) + path extra_files + + output: + tuple val(meta), path("*.vcf.gz") , optional:true, emit: vcf + tuple val(meta), path("*.tab.gz") , optional:true, emit: tab + tuple val(meta), path("*.json.gz") , optional:true, emit: json + path "*.summary.html" , optional:true, emit: report + path "versions.yml" , emit: versions + + when: + task.ext.when == null || task.ext.when + + script: + def args = task.ext.args ?: '' + def file_extension = args.contains("--vcf") ? 'vcf' : args.contains("--json")? 'json' : args.contains("--tab")? 'tab' : 'vcf' + def compress_cmd = args.contains("--compress_output") ? '' : '--compress_output bgzip' + def prefix = task.ext.prefix ?: "${meta.id}" + def dir_cache = cache ? "\${PWD}/${cache}" : "/.vep" + def reference = fasta ? "--fasta $fasta" : "" + """ + vep \\ + -i $vcf \\ + -o ${prefix}.${file_extension}.gz \\ + $args \\ + $compress_cmd \\ + $reference \\ + --assembly $genome \\ + --species $species \\ + --cache \\ + --cache_version $cache_version \\ + --dir_cache $dir_cache \\ + --fork $task.cpus + + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + ensemblvep: \$( echo \$(vep --help 2>&1) | sed 's/^.*Versions:.*ensembl-vep : //;s/ .*\$//') + END_VERSIONS + """ + + stub: + def prefix = task.ext.prefix ?: "${meta.id}" + """ + echo "" | gzip > ${prefix}.vcf.gz + echo "" | gzip > ${prefix}.tab.gz + echo "" | gzip > ${prefix}.json.gz + touch ${prefix}.summary.html + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + ensemblvep: \$( echo \$(vep --help 2>&1) | sed 's/^.*Versions:.*ensembl-vep : //;s/ .*\$//') + END_VERSIONS + """ +} diff --git a/modules/nf-core/ensemblvep/vep/meta.yml b/modules/nf-core/ensemblvep/vep/meta.yml new file mode 100644 index 0000000..d8ff8d1 --- /dev/null +++ b/modules/nf-core/ensemblvep/vep/meta.yml @@ -0,0 +1,92 @@ +name: ensemblvep_vep +description: Ensembl Variant Effect Predictor (VEP). The output-file-format is controlled through `task.ext.args`. +keywords: + - annotation + - vcf + - json + - tab +tools: + - ensemblvep: + description: | + VEP determines the effect of your variants (SNPs, insertions, deletions, CNVs + or structural variants) on genes, transcripts, and protein sequence, as well as regulatory regions. + homepage: https://www.ensembl.org/info/docs/tools/vep/index.html + documentation: https://www.ensembl.org/info/docs/tools/vep/script/index.html + licence: ["Apache-2.0"] +input: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - vcf: + type: file + description: | + vcf to annotate + - custom_extra_files: + type: file + description: | + extra sample-specific files to be used with the `--custom` flag to be configured with ext.args + (optional) + - genome: + type: string + description: | + which genome to annotate with + - species: + type: string + description: | + which species to annotate with + - cache_version: + type: integer + description: | + which version of the cache to annotate with + - cache: + type: file + description: | + path to VEP cache (optional) + - meta2: + type: map + description: | + Groovy Map containing fasta reference information + e.g. [ id:'test' ] + - fasta: + type: file + description: | + reference FASTA file (optional) + pattern: "*.{fasta,fa}" + - extra_files: + type: file + description: | + path to file(s) needed for plugins (optional) +output: + - vcf: + type: file + description: | + annotated vcf (optional) + pattern: "*.ann.vcf.gz" + - tab: + type: file + description: | + tab file with annotated variants (optional) + pattern: "*.ann.tab.gz" + - json: + type: file + description: | + json file with annotated variants (optional) + pattern: "*.ann.json.gz" + - report: + type: file + description: VEP report file + pattern: "*.html" + - versions: + type: file + description: File containing software versions + pattern: "versions.yml" +authors: + - "@maxulysse" + - "@matthdsm" + - "@nvnieuwk" +maintainers: + - "@maxulysse" + - "@matthdsm" + - "@nvnieuwk" diff --git a/modules/nf-core/ensemblvep/vep/tests/main.nf.test b/modules/nf-core/ensemblvep/vep/tests/main.nf.test new file mode 100644 index 0000000..4aff84a --- /dev/null +++ b/modules/nf-core/ensemblvep/vep/tests/main.nf.test @@ -0,0 +1,114 @@ +nextflow_process { + + name "Test Process ENSEMBLVEP_VEP" + script "../main.nf" + process "ENSEMBLVEP_VEP" + config "./nextflow.config" + + tag "modules" + tag "modules_nfcore" + tag "ensemblvep" + tag "ensemblvep/vep" + tag "ensemblvep/download" + + test("test_ensemblvep_vep_fasta_vcf") { + config "./vcf.config" + + setup { + run("ENSEMBLVEP_DOWNLOAD") { + script "../../download/main.nf" + + process { + """ + input[0] = Channel.of([ + [id:"111_WBcel235"], + params.vep_genome, + params.vep_species, + params.vep_cache_version + ]) + """ + } + } + } + + when { + process { + """ + input[0] = Channel.of([ + [ id:'test' ], // meta map + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/vcf/test.vcf', checkIfExists: true), + [] + ]) + input[1] = params.vep_genome + input[2] = params.vep_species + input[3] = params.vep_cache_version + input[4] = ENSEMBLVEP_DOWNLOAD.out.cache.map{ meta, cache -> [cache] } + input[5] = Channel.value([ + [id:"fasta"], + file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta', checkIfExists: true) + ]) + input[6] = [] + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(process.out.versions).match() }, + { assert path(process.out.vcf.get(0).get(1)).linesGzip.contains("##fileformat=VCFv4.2") } + ) + } + + } + + test("test_ensemblvep_vep_fasta_tab_gz") { + config "./tab.gz.config" + + setup { + run("ENSEMBLVEP_DOWNLOAD") { + script "../../download/main.nf" + + process { + """ + input[0] = Channel.of([ + [id:"111_WBcel235"], + params.vep_genome, + params.vep_species, + params.vep_cache_version + ]) + """ + } + } + } + + when { + process { + """ + input[0] = Channel.of([ + [ id:'test' ], // meta map + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/vcf/test.vcf', checkIfExists: true), + [] + ]) + input[1] = params.vep_genome + input[2] = params.vep_species + input[3] = params.vep_cache_version + input[4] = ENSEMBLVEP_DOWNLOAD.out.cache.map{ meta, cache -> [cache] } + input[5] = Channel.value([ + [id:"fasta"], + file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta', checkIfExists: true) + ]) + input[6] = [] + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(process.out.versions).match() }, + { assert path(process.out.tab.get(0).get(1)).linesGzip.contains("## ENSEMBL VARIANT EFFECT PREDICTOR v111.0") } + ) + } + } +} diff --git a/modules/nf-core/ensemblvep/vep/tests/main.nf.test.snap b/modules/nf-core/ensemblvep/vep/tests/main.nf.test.snap new file mode 100644 index 0000000..f937b29 --- /dev/null +++ b/modules/nf-core/ensemblvep/vep/tests/main.nf.test.snap @@ -0,0 +1,26 @@ +{ + "test_ensemblvep_vep_fasta_tab_gz": { + "content": [ + [ + "versions.yml:md5,bd2ba1b4741a7d0a224160b50859f4ba" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "24.03.0" + }, + "timestamp": "2024-04-15T17:35:20.694114" + }, + "test_ensemblvep_vep_fasta_vcf": { + "content": [ + [ + "versions.yml:md5,bd2ba1b4741a7d0a224160b50859f4ba" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "24.03.0" + }, + "timestamp": "2024-04-15T17:34:41.093843" + } +} \ No newline at end of file diff --git a/modules/nf-core/ensemblvep/vep/tests/nextflow.config b/modules/nf-core/ensemblvep/vep/tests/nextflow.config new file mode 100644 index 0000000..882bce4 --- /dev/null +++ b/modules/nf-core/ensemblvep/vep/tests/nextflow.config @@ -0,0 +1,12 @@ +params { + vep_cache_version = "111" + vep_genome = "WBcel235" + vep_species = "caenorhabditis_elegans" +} + +process { + withName: ENSEMBLVEP_DOWNLOAD { + ext.args = '--AUTO c --CONVERT --NO_BIOPERL --NO_HTSLIB --NO_TEST --NO_UPDATE' + ext.prefix = { "${params.vep_cache_version}_${params.vep_genome}" } + } +} diff --git a/modules/nf-core/ensemblvep/vep/tests/tab.gz.config b/modules/nf-core/ensemblvep/vep/tests/tab.gz.config new file mode 100644 index 0000000..40eb03e --- /dev/null +++ b/modules/nf-core/ensemblvep/vep/tests/tab.gz.config @@ -0,0 +1,5 @@ +process { + withName: ENSEMBLVEP_VEP { + ext.args = '--tab --compress_output bgzip' + } +} diff --git a/modules/nf-core/ensemblvep/vep/tests/tags.yml b/modules/nf-core/ensemblvep/vep/tests/tags.yml new file mode 100644 index 0000000..4aa4aa4 --- /dev/null +++ b/modules/nf-core/ensemblvep/vep/tests/tags.yml @@ -0,0 +1,2 @@ +ensemblvep/vep: + - "modules/nf-core/ensemblvep/vep/**" diff --git a/modules/nf-core/ensemblvep/vep/tests/vcf.config b/modules/nf-core/ensemblvep/vep/tests/vcf.config new file mode 100644 index 0000000..ad8955a --- /dev/null +++ b/modules/nf-core/ensemblvep/vep/tests/vcf.config @@ -0,0 +1,5 @@ +process { + withName: ENSEMBLVEP_VEP { + ext.args = '--vcf' + } +} diff --git a/modules/nf-core/samtools/convert/main.nf b/modules/nf-core/samtools/convert/main.nf index 3c539e4..d61fbe2 100644 --- a/modules/nf-core/samtools/convert/main.nf +++ b/modules/nf-core/samtools/convert/main.nf @@ -42,4 +42,19 @@ process SAMTOOLS_CONVERT { samtools: \$(echo \$(samtools --version 2>&1) | sed 's/^.*samtools //; s/Using.*\$//') END_VERSIONS """ + + stub: + def prefix = task.ext.prefix ?: "${meta.id}" + def output_extension = input.getExtension() == "bam" ? "cram" : "bam" + def index_extension = output_extension == "bam" ? "bai" : "crai" + + """ + touch ${prefix}.${output_extension} + touch ${prefix}.${index_extension} + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + samtools: \$(echo \$(samtools --version 2>&1) | sed 's/^.*samtools //; s/Using.*\$//') + END_VERSIONS + """ } diff --git a/modules/nf-core/samtools/convert/tests/main.nf.test b/modules/nf-core/samtools/convert/tests/main.nf.test index d2ab583..91a0c69 100644 --- a/modules/nf-core/samtools/convert/tests/main.nf.test +++ b/modules/nf-core/samtools/convert/tests/main.nf.test @@ -72,4 +72,36 @@ nextflow_process { ) } } + + test("sarscov2 - [bam, bai], fasta, fai - stub") { + + options "-stub" + + when { + process { + """ + input[0] = Channel.of([ + [id: 'test', single_end: false], // meta map + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/bam/test.paired_end.sorted.bam', checkIfExists: true), + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/bam/test.paired_end.sorted.bam.bai', checkIfExists: true) + ]) + input[1] = Channel.of([ + [ id:'fasta' ], // meta map + file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta', checkIfExists: true) + ]) + input[2] = Channel.of([ + [ id:'fai' ], // meta map + file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta.fai', checkIfExists: true) + ]) + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(process.out).match("stub") } + ) + } + } } diff --git a/modules/nf-core/samtools/convert/tests/main.nf.test.snap b/modules/nf-core/samtools/convert/tests/main.nf.test.snap index 9d56a4e..3d6f88c 100644 --- a/modules/nf-core/samtools/convert/tests/main.nf.test.snap +++ b/modules/nf-core/samtools/convert/tests/main.nf.test.snap @@ -43,6 +43,71 @@ }, "timestamp": "2024-03-06T11:14:36.643960504" }, + "stub": { + "content": [ + { + "0": [ + + ], + "1": [ + [ + { + "id": "test", + "single_end": false + }, + "test.cram:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "2": [ + + ], + "3": [ + [ + { + "id": "test", + "single_end": false + }, + "test.crai:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "4": [ + "versions.yml:md5,dd0aaf61f39c9ce91c74090f3c7ce1d0" + ], + "bai": [ + + ], + "bam": [ + + ], + "crai": [ + [ + { + "id": "test", + "single_end": false + }, + "test.crai:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "cram": [ + [ + { + "id": "test", + "single_end": false + }, + "test.cram:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "versions": [ + "versions.yml:md5,dd0aaf61f39c9ce91c74090f3c7ce1d0" + ] + } + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "24.03.0" + }, + "timestamp": "2024-05-03T12:01:40.896170698" + }, "bam_to_cram_index": { "content": [ "test.cram.crai" diff --git a/nextflow.config b/nextflow.config index f22b5b9..edc6de6 100644 --- a/nextflow.config +++ b/nextflow.config @@ -62,6 +62,15 @@ params { // ExomeDepth Parameters roi_auto = null roi_chrx = null + exomedepth = false + yamlconfig = "${projectDir}/assets/exomedepth.yaml" + + // VEP Parameters + vep_cache_version = 105 + species = "homo_sapiens" + vep_cache = null + annotate = null + vep_assembly = "GRCh38" } diff --git a/nextflow_schema.json b/nextflow_schema.json index 7ed9948..30e7a23 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -40,6 +40,10 @@ "type": "string", "description": "MultiQC report title. Printed as page header, used for filename if not otherwise specified.", "fa_icon": "fas fa-file-signature" + }, + "exomedepth": { + "type": "boolean", + "description": "Option to execute the ExomeDepth tool" } } }, @@ -283,6 +287,11 @@ "description": "Validation of parameters in lenient more.", "hidden": true, "help_text": "Allows string values that are parseable as numbers or booleans. For further information see [JSONSchema docs](https://github.com/everit-org/json-schema#lenient-mode)." + }, + "yamlconfig": { + "type": "string", + "description": "YAML config file to use in BEDGOVCF module", + "default": "/home/bertg/exomecnv/assets/exomedepth.yaml" } } }, @@ -297,19 +306,49 @@ "description": "Path to the default ROI (regions of interest) BED file of autosomal (merged) regions to be used for CNV analysis", "format": "file-path", "pattern": "^\\S+\\.bed$", - "mimetype": "text/plain", - "default": null + "mimetype": "text/plain" }, "roi_chrx": { "type": "string", "description": "Path to the default ROI (regions of interest) BED file of chrX (merged) regions to be used for CNV analysis", "format": "file-path", "pattern": "^\\S+\\.bed$", - "mimetype": "text/plain", - "default": null + "mimetype": "text/plain" } }, "required": ["roi_auto", "roi_chrx"] + }, + "vep_parameters": { + "title": "VEP parameters", + "type": "object", + "description": "", + "default": "", + "properties": { + "vep_cache_version": { + "type": "integer", + "description": "Cache version", + "default": 105 + }, + "species": { + "type": "string", + "description": "Species", + "default": "homo_sapiens" + }, + "vep_cache": { + "type": "string", + "description": "Path to the VEP cache" + }, + "annotate": { + "type": "string", + "description": "Path to VCF file to run VEP on" + }, + "vep_assembly": { + "type": "string", + "description": "Genome", + "default": "GRCh38" + } + }, + "required": ["vep_cache"] } }, "allOf": [ @@ -330,6 +369,9 @@ }, { "$ref": "#/definitions/exomedepth_parameters" + }, + { + "$ref": "#/definitions/vep_parameters" } ] } diff --git a/subworkflows/local/bam_variant_calling_exomedepth/main.nf b/subworkflows/local/bam_variant_calling_exomedepth/main.nf deleted file mode 100644 index 4757926..0000000 --- a/subworkflows/local/bam_variant_calling_exomedepth/main.nf +++ /dev/null @@ -1,37 +0,0 @@ -// TODO nf-core: If in doubt look at other nf-core/subworkflows to see how we are doing things! :) -// https://github.com/nf-core/modules/tree/master/subworkflows -// You can also ask for help via your pull request or on the #subworkflows channel on the nf-core Slack workspace: -// https://nf-co.re/join -// TODO nf-core: A subworkflow SHOULD import at least two modules - -// Convert CRAM files (optional) -include { SAMTOOLS_CONVERT as CRAM_TO_BAM } from '../../../modules/nf-core/samtools/convert/main' -// include { SAMTOOLS_INDEX } from '../../../modules/nf-core/samtools/index/main' - -workflow BAM_VARIANT_CALLING_EXOMEDEPTH { - - take: - // TODO nf-core: edit input (take) channels - ch_bam // channel: [ val(meta), [ bam ] ] - - main: - - ch_versions = Channel.empty() - - // TODO nf-core: substitute modules here for the modules of your subworkflow - - SAMTOOLS_SORT ( ch_bam ) - ch_versions = ch_versions.mix(SAMTOOLS_SORT.out.versions.first()) - - SAMTOOLS_INDEX ( SAMTOOLS_SORT.out.bam ) - ch_versions = ch_versions.mix(SAMTOOLS_INDEX.out.versions.first()) - - emit: - // TODO nf-core: edit emitted channels - bam = SAMTOOLS_SORT.out.bam // channel: [ val(meta), [ bam ] ] - bai = SAMTOOLS_INDEX.out.bai // channel: [ val(meta), [ bai ] ] - csi = SAMTOOLS_INDEX.out.csi // channel: [ val(meta), [ csi ] ] - - versions = ch_versions // channel: [ versions.yml ] -} - diff --git a/subworkflows/local/cram_prepare/main.nf b/subworkflows/local/cram_prepare/main.nf deleted file mode 100644 index 275e672..0000000 --- a/subworkflows/local/cram_prepare/main.nf +++ /dev/null @@ -1,28 +0,0 @@ - -// Convert CRAM files -include { SAMTOOLS_CONVERT as CRAM_TO_BAM } from '../../../modules/nf-core/samtools/convert/main' - -workflow CRAM_PREPARE { - - take: - ch_cram // channel: [mandatory] [ val(meta), path(cram), path(crai) ] => sample CRAM files and their optional indices - fasta // channel: [mandatory] fasta - fasta_fai // channel: [mandatory] fasta_fai - - main: - - ch_versions = Channel.empty() - - // - // convert CRAM files to BAM files - // - CRAM_TO_BAM(ch_cram, fasta, fasta_fai) - ch_versions = ch_versions.mix(CRAM_TO_BAM.out.versions) - - emit: - bam = CRAM_TO_BAM.out.bam // channel: [ val(meta), [ bam ] ] - bai = CRAM_TO_BAM.out.bai // channel: [ val(meta), [ bai ] ] - - versions = ch_versions // channel: [ versions.yml ] -} - diff --git a/subworkflows/local/exomedepth/main.nf b/subworkflows/local/exomedepth/main.nf new file mode 100644 index 0000000..3345126 --- /dev/null +++ b/subworkflows/local/exomedepth/main.nf @@ -0,0 +1,157 @@ +/* +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ + IMPORT MODULES / SUBWORKFLOWS +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ +*/ + +include { SAMTOOLS_CONVERT as CRAM_PREPARE } from '../../../modules/nf-core/samtools/convert/main' +include { COUNT as COUNT_X } from '../../../modules/local/exomedepth/count/main' +include { COUNT as COUNT_AUTO } from '../../../modules/local/exomedepth/count/main' +include { COUNT_MERGE as COUNT_MERGE_AUTO } from '../../../modules/local/exomedepth/merge_count/main' +include { COUNT_MERGE as COUNT_MERGE_X } from '../../../modules/local/exomedepth/merge_count/main' +include { CNV_CALL as CNV_CALL_AUTO } from '../../../modules/local/exomedepth/cnv_call/main' +include { CNV_CALL as CNV_CALL_X } from '../../../modules/local/exomedepth/cnv_call/main' +include { CNV_MERGE } from '../../../modules/local/exomedepth/merge_cnv/main' +include { BEDGOVCF } from '../../../modules/nf-core/bedgovcf/main' + +/* +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ + RUN EXOMEDEPTH WORKFLOW +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ +*/ + +workflow EXOMEDEPTH { + + take: + ch_samplesheet + + main: + ch_versions = Channel.empty() + + // Importing and convert the input files passed through the parameters to channels + // Branch into CRAM and BAM files + + ch_samplesheet.branch { meta, cram, crai -> + CRAM: cram.extension == "cram" + BAM: cram.extension == "bam" + } + .set{ ch_input_prepare } + + ch_fasta = Channel.fromPath(params.fasta).map{ [[id:"reference"], it]}.collect() + ch_fai = params.fai ? Channel.fromPath(params.fai).map{ [[id:"reference"], it]}.collect() : null + ch_roi_auto = Channel.fromPath(params.roi_auto).map{ [[id:"autosomal"], it]}.collect() + ch_roi_x = Channel.fromPath(params.roi_chrx).map{ [[id:"chrX"], it]}.collect() + + // SUBWORKFLOW: Convert CRAM to BAM if no BAM file was provided + + CRAM_PREPARE ( + ch_input_prepare.CRAM, ch_fasta, ch_fai + ) + ch_versions = ch_versions.mix(CRAM_PREPARE.out.versions) + + CRAM_PREPARE.out.bam + .join(CRAM_PREPARE.out.bai) + .set{ ch_cram_prepare } + + ch_input_prepare.BAM + .mix(ch_cram_prepare) + .set{ ch_input_bam } + + //MODULE: Count autosomal reads per sample (count file for each sample) + + COUNT_AUTO ( + ch_input_bam, ch_roi_auto + ) + ch_versions = ch_versions.mix(COUNT_AUTO.out.versions) + + //MODULE: Group autosomal counts per pool (count file for each pool) + + grouped_counts_auto = COUNT_AUTO.out.counts + .map { meta, txt -> + def new_meta = [id:meta.pool] + [new_meta, meta.sample, meta.family, txt] + } + .groupTuple() + .map { meta, samples, families, txt -> + def new_meta = [id:meta.id, chr:"autosomal", sam:samples, fam: families] + [new_meta, txt] + } + + COUNT_MERGE_AUTO ( + grouped_counts_auto + ) + + //MODULE: Autosomal CNV call per sample (file for each sample) + + cnv_auto_ch = COUNT_MERGE_AUTO.out + .map { meta, txt -> + [meta, meta.sam, txt] + } + .transpose(by:1) + + CNV_CALL_AUTO( + ch_roi_auto, cnv_auto_ch + ) + ch_versions = ch_versions.mix(CNV_CALL_AUTO.out.versions) + + //MODULE: Count chrX reads per sample (count file for each sample) + + COUNT_X ( + ch_input_bam, ch_roi_x + ) + + //MODULE: Group chrX counts per pool (count file for each pool) + + grouped_counts_X = COUNT_X.out.counts + .map { meta, txt -> + def new_meta = [id:meta.pool] + [new_meta, meta.sample, meta.family, txt] + } + .groupTuple() + .map { meta, samples, families, txt -> + def new_meta = [id:meta.id, chr:"chrX", sam:samples, fam: families] + [new_meta, txt] + } + + COUNT_MERGE_X ( + grouped_counts_X + ) + + //MODULE: ChrX CNV call per sample (file for each sample) + + cnv_chrx_ch = COUNT_MERGE_X.out + .map { meta, txt -> + [meta, meta.sam, txt] + } + .transpose(by:1) + + CNV_CALL_X( + ch_roi_auto, cnv_chrx_ch + ) + + //MODULE: Group autosomal and chrX CNV per sample (one file for each sample) + + cnv_merge_ch = CNV_CALL_AUTO.out.cnvcall + .combine(CNV_CALL_X.out.cnvcall, by:1) + .map{ sample, meta, auto, meta2, x -> + [sample, auto, x] + } + + CNV_MERGE(cnv_merge_ch) + + //MODULE: Convert file to VCF according to a YAML config + + bedgovcf_input = CNV_MERGE.out + .map{ meta, bed -> + def new_meta = [id:meta] + [new_meta, bed, params.yamlconfig] + } + + BEDGOVCF(bedgovcf_input, ch_fai) + ch_versions = ch_versions.mix(BEDGOVCF.out.versions) + + emit: + versions = ch_versions + vcf = BEDGOVCF.out.vcf +} + diff --git a/subworkflows/local/vcf_annotation/main.nf b/subworkflows/local/vcf_annotation/main.nf new file mode 100644 index 0000000..ccb0e5c --- /dev/null +++ b/subworkflows/local/vcf_annotation/main.nf @@ -0,0 +1,39 @@ +/* +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ + IMPORT MODULES / SUBWORKFLOWS +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ +*/ + +include { ENSEMBLVEP_VEP as VEP } from '../../../modules/nf-core/ensemblvep/vep/main' + +/* +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ + RUN ENSEMBLE VEP WORKFLOW +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ +*/ + +workflow ENSEMBLVEP { + + take: + + ch_vcfs + fasta + vep_cache + + main: + + ch_vep_extra_files = [] + + VEP ( + ch_vcfs, + params.vep_assembly, + params.species, + params.vep_cache_version, + vep_cache, + fasta, + ch_vep_extra_files + ) + + emit: + vcfs = VEP.out.vcf +} diff --git a/subworkflows/nf-core/utils_nfcore_pipeline/main.nf b/subworkflows/nf-core/utils_nfcore_pipeline/main.nf index a8b55d6..14558c3 100644 --- a/subworkflows/nf-core/utils_nfcore_pipeline/main.nf +++ b/subworkflows/nf-core/utils_nfcore_pipeline/main.nf @@ -65,9 +65,15 @@ def checkProfileProvided(nextflow_cli_args) { // Citation string for pipeline // def workflowCitation() { + def temp_doi_ref = "" + String[] manifest_doi = workflow.manifest.doi.tokenize(",") + // Using a loop to handle multiple DOIs + // Removing `https://doi.org/` to handle pipelines using DOIs vs DOI resolvers + // Removing ` ` since the manifest.doi is a string and not a proper list + for (String doi_ref: manifest_doi) temp_doi_ref += " https://doi.org/${doi_ref.replace('https://doi.org/', '').replace(' ', '')}\n" return "If you use ${workflow.manifest.name} for your analysis please cite:\n\n" + "* The pipeline\n" + - " ${workflow.manifest.doi}\n\n" + + temp_doi_ref + "\n" + "* The nf-core framework\n" + " https://doi.org/10.1038/s41587-020-0439-x\n\n" + "* Software dependencies\n" + diff --git a/workflows/exomecnv.nf b/workflows/exomecnv.nf index 79f72e4..5308536 100644 --- a/workflows/exomecnv.nf +++ b/workflows/exomecnv.nf @@ -11,17 +11,9 @@ include { softwareVersionsToYAML } from '../subworkflows/nf-core/utils_nfcore_pi include { methodsDescriptionText } from '../subworkflows/local/utils_nfcore_exomecnv_pipeline' // local -include { CRAM_PREPARE } from '../subworkflows/local/cram_prepare/main' -include { COUNT as COUNT_X } from '../modules/local/exomedepth/count/main' -include { COUNT as COUNT_AUTO } from '../modules/local/exomedepth/count/main' -include { COUNT_MERGE as COUNT_MERGE_AUTO } from '../modules/local/exomedepth/merge_count/main' -include { COUNT_MERGE as COUNT_MERGE_X } from '../modules/local/exomedepth/merge_count/main' -include { CNV_CALL as CNV_CALL_AUTO } from '../modules/local/exomedepth/cnv_call/main' -include { CNV_CALL as CNV_CALL_X } from '../modules/local/exomedepth/cnv_call/main' -include { CNV_MERGE } from '../modules/local/exomedepth/merge_cnv/main' -include { BEDGOVCF } from '../modules/nf-core/bedgovcf/main' - -// include { BAM_VARIANT_CALLING_EXOMEDEPTH } from '../subworkflows/local/bam_variant_calling_exomedepth/main' +include { EXOMEDEPTH } from '../subworkflows/local/exomedepth/main' +include { ENSEMBLVEP } from '../subworkflows/local/vcf_annotation/main' + /* ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ RUN MAIN WORKFLOW @@ -37,141 +29,55 @@ workflow EXOMECNV { ch_versions = Channel.empty() ch_multiqc_files = Channel.empty() - // Importing and convert the input files passed through the parameters to channels - // Branch into CRAM and BAM files - - ch_samplesheet.branch { meta, cram, crai -> - CRAM: cram.extension == "cram" - BAM: cram.extension == "bam" - } - .set{ ch_input_prepare } - - ch_fasta = Channel.fromPath(params.fasta).map{ [[id:"reference"], it]}.collect() - ch_fai = params.fai ? Channel.fromPath(params.fai).map{ [[id:"reference"], it]}.collect() : null - ch_roi_auto = Channel.fromPath(params.roi_auto).map{ [[id:"autosomal"], it]}.collect() - ch_roi_x = Channel.fromPath(params.roi_chrx).map{ [[id:"chrX"], it]}.collect() - - // SUBWORKFLOW: Convert CRAM to BAM if no BAM file was provided - - CRAM_PREPARE ( - ch_input_prepare.CRAM, ch_fasta, ch_fai - ) - - CRAM_PREPARE.out.bam - .join(CRAM_PREPARE.out.bai) - .set{ ch_cram_prepare } - - ch_input_prepare.BAM - .mix(ch_cram_prepare) - .set{ ch_input_bam } - - //MODULE: Count autosomal reads per sample (count file for each sample) - - if (params.exomedepth) { - - COUNT_AUTO ( - ch_input_bam, ch_roi_auto - ) - ch_versions = ch_versions.mix(COUNT_AUTO.out.versions) - - //MODULE: Group autosomal counts per pool (count file for each pool) - - grouped_counts_auto = COUNT_AUTO.out.counts - .map { meta, txt -> - def new_meta = [id:meta.pool] - [new_meta, meta.sample, meta.family, txt] - } - .groupTuple() - .map { meta, samples, families, txt -> - def new_meta = [id:meta.id, chr:"autosomal", sam:samples, fam: families] - [new_meta, txt] + ch_fasta = channel.fromPath(params.fasta) + .map{ path -> + def new_meta = [id:"reference"] + [new_meta, path] } + .collect() - COUNT_MERGE_AUTO ( - grouped_counts_auto - ) + ch_vep_cache = Channel.fromPath(params.vep_cache).collect() - //MODULE: Autosomal CNV call per sample (file for each sample) + if (!params.annotate){ - cnv_auto_ch = COUNT_MERGE_AUTO.out - .map { meta, txt -> - [meta, meta.sam, txt] - } - .transpose(by:1) + // ExomeDepth - CNV_CALL_AUTO( - ch_roi_auto, cnv_auto_ch - ) - ch_versions = ch_versions.mix(CNV_CALL_AUTO.out.versions) - - //MODULE: Count chrX reads per sample (count file for each sample) - - COUNT_X ( - ch_input_bam, ch_roi_x - ) - - //MODULE: Group chrX counts per pool (count file for each pool) - - grouped_counts_X = COUNT_X.out.counts - .map { meta, txt -> - def new_meta = [id:meta.pool] - [new_meta, meta.sample, meta.family, txt] - } - .groupTuple() - .map { meta, samples, families, txt -> - def new_meta = [id:meta.id, chr:"chrX", sam:samples, fam: families] - [new_meta, txt] - } - - COUNT_MERGE_X ( - grouped_counts_X - ) + if (params.exomedepth) { + EXOMEDEPTH (ch_samplesheet) + ch_versions = EXOMEDEPTH.out.versions + + // EnsemblVEP after ExomeDepth + ch_empty = [] + ch_exomedepth_vcf = EXOMEDEPTH.out.vcf + .map { meta, path -> + [meta, path, ch_empty]} + ENSEMBLVEP ( ch_exomedepth_vcf, ch_fasta, ch_vep_cache ) + } + } - //MODULE: ChrX CNV call per sample (file for each sample) + // EnsemblVEP on VCF input file - cnv_chrx_ch = COUNT_MERGE_X.out - .map { meta, txt -> - [meta, meta.sam, txt] - } - .transpose(by:1) + else { + ch_empty = [] + ch_vcf = Channel.fromPath(params.annotate) + .splitCsv( header:true) + .map { row -> + [[id:row.sample], row.vcf, ch_empty]} + .collect() - CNV_CALL_X( - ch_roi_auto, cnv_chrx_ch + ENSEMBLVEP ( + ch_vcf, ch_fasta, ch_vep_cache ) - - //MODULE: Group autosomal and chrX CNV per sample (one file for each sample) - - cnv_merge_ch = CNV_CALL_AUTO.out.cnvcall - .combine(CNV_CALL_X.out.cnvcall, by:1) - .map{ sample, meta, auto, meta2, x -> - [sample, auto, x] - } - - CNV_MERGE(cnv_merge_ch) - - //MODULE: Convert file to VCF according to a YAML config - - bedgovcf_input = CNV_MERGE.out - .map{ meta, bed -> - def new_meta = [id:meta] - [new_meta, bed, params.yamlconfig] - } - - BEDGOVCF(bedgovcf_input, ch_fai) - ch_versions = ch_versions.mix(BEDGOVCF.out.versions) - } - // Collate and save software versions - // + softwareVersionsToYAML(ch_versions) .collectFile(storeDir: "${params.outdir}/pipeline_info", name: 'nf_core_pipeline_software_mqc_versions.yml', sort: true, newLine: true) .set { ch_collated_versions } - // // MODULE: MultiQC - // + ch_multiqc_config = Channel.fromPath("$projectDir/assets/multiqc_config.yml", checkIfExists: true) ch_multiqc_custom_config = params.multiqc_config ? Channel.fromPath(params.multiqc_config, checkIfExists: true) : Channel.empty() ch_multiqc_logo = params.multiqc_logo ? Channel.fromPath(params.multiqc_logo, checkIfExists: true) : Channel.empty() From d2783295b2f31dad616d6ba930ed270eb66043c2 Mon Sep 17 00:00:00 2001 From: BertGalle Date: Tue, 7 May 2024 11:38:55 +0200 Subject: [PATCH 43/64] Config --- nextflow.config | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/nextflow.config b/nextflow.config index edc6de6..d1de4cc 100644 --- a/nextflow.config +++ b/nextflow.config @@ -63,7 +63,7 @@ params { roi_auto = null roi_chrx = null exomedepth = false - yamlconfig = "${projectDir}/assets/exomedepth.yaml" + yamlconfig = null // VEP Parameters vep_cache_version = 105 From 8c6fc856e6a1c68034a2c072bc2975de9a401f37 Mon Sep 17 00:00:00 2001 From: BertGalle Date: Tue, 7 May 2024 11:49:07 +0200 Subject: [PATCH 44/64] Config again --- assets/exomedepth.yaml | 1 - nextflow_schema.json | 3 +-- 2 files changed, 1 insertion(+), 3 deletions(-) diff --git a/assets/exomedepth.yaml b/assets/exomedepth.yaml index 48e4d31..296038d 100644 --- a/assets/exomedepth.yaml +++ b/assets/exomedepth.yaml @@ -51,4 +51,3 @@ info: value: $12 type: string description: Exons - diff --git a/nextflow_schema.json b/nextflow_schema.json index 30e7a23..d1d881c 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -290,8 +290,7 @@ }, "yamlconfig": { "type": "string", - "description": "YAML config file to use in BEDGOVCF module", - "default": "/home/bertg/exomecnv/assets/exomedepth.yaml" + "description": "YAML config file to use in BEDGOVCF module" } } }, From b00e4de69f516152ad39a20086adf29074738922 Mon Sep 17 00:00:00 2001 From: BertGalle Date: Tue, 7 May 2024 13:44:36 +0200 Subject: [PATCH 45/64] Stub --- .github/workflows/ci.yml | 2 +- conf/modules.config | 1 + modules/local/exomedepth/cnv_call/main.nf | 12 ++++++++++++ modules/local/exomedepth/count/main.nf | 11 +++++++++++ modules/local/exomedepth/merge_cnv/main.nf | 5 +++++ modules/local/exomedepth/merge_count/main.nf | 6 ++++++ nextflow_schema.json | 8 ++++---- 7 files changed, 40 insertions(+), 5 deletions(-) diff --git a/.github/workflows/ci.yml b/.github/workflows/ci.yml index 0cdd2da..07eee52 100644 --- a/.github/workflows/ci.yml +++ b/.github/workflows/ci.yml @@ -43,4 +43,4 @@ jobs: # For example: adding multiple test runs with different parameters # Remember that you can parallelise this by using strategy.matrix run: | - nextflow run ${GITHUB_WORKSPACE} -profile test,docker --outdir ./results + nextflow run ${GITHUB_WORKSPACE} -profile test,docker --outdir ./results -stub diff --git a/conf/modules.config b/conf/modules.config index 8e02374..4e15f97 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -63,6 +63,7 @@ process { '--merged', '--exclude_predicted', '--fields "SYMBOL,Gene"', + '--transcript_filter "stable_id match ENST"' ].join(' ').trim()} } } diff --git a/modules/local/exomedepth/cnv_call/main.nf b/modules/local/exomedepth/cnv_call/main.nf index cdb512f..8c91ab1 100644 --- a/modules/local/exomedepth/cnv_call/main.nf +++ b/modules/local/exomedepth/cnv_call/main.nf @@ -35,4 +35,16 @@ process CNV_CALL { R: \$(Rscript --version | sed 's/R scripting front-end //g') END_VERSIONS """ + + stub: + def prefix = task.ext.prefix ?: "${sample}_CNVs_ExomeDepth_${meta.id}" + """ + touch ${prefix}.txt + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + ExomeDepth: ${VERSION} + R: \$(Rscript --version | sed 's/R scripting front-end //g') + END_VERSIONS + """ } diff --git a/modules/local/exomedepth/count/main.nf b/modules/local/exomedepth/count/main.nf index 31bf183..4c1f14e 100644 --- a/modules/local/exomedepth/count/main.nf +++ b/modules/local/exomedepth/count/main.nf @@ -39,4 +39,15 @@ process COUNT { END_VERSIONS """ + + stub: + """ + touch ${meta.id}_${meta2.id}.txt + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + ExomeDepth: ${VERSION} + R: \$(Rscript --version | sed 's/R scripting front-end //g') + END_VERSIONS + """ } diff --git a/modules/local/exomedepth/merge_cnv/main.nf b/modules/local/exomedepth/merge_cnv/main.nf index 3ff104d..c4036c1 100644 --- a/modules/local/exomedepth/merge_cnv/main.nf +++ b/modules/local/exomedepth/merge_cnv/main.nf @@ -17,4 +17,9 @@ process CNV_MERGE { tail +2 $chrx >> "${prefix}.txt" """ + stub: + def prefix = task.ext.prefix ?: "${meta}_CNVs_ExomeDepth" + """ + touch ${prefix}.txt + """ } diff --git a/modules/local/exomedepth/merge_count/main.nf b/modules/local/exomedepth/merge_count/main.nf index ae2e255..0192d5e 100644 --- a/modules/local/exomedepth/merge_count/main.nf +++ b/modules/local/exomedepth/merge_count/main.nf @@ -22,4 +22,10 @@ process COUNT_MERGE { fi done """ + + stub: + def prefix = task.ext.prefix ?: "${meta.id}_${meta.chr}" + """ + touch ${prefix}.txt + """ } diff --git a/nextflow_schema.json b/nextflow_schema.json index d1d881c..ed4258c 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -287,10 +287,6 @@ "description": "Validation of parameters in lenient more.", "hidden": true, "help_text": "Allows string values that are parseable as numbers or booleans. For further information see [JSONSchema docs](https://github.com/everit-org/json-schema#lenient-mode)." - }, - "yamlconfig": { - "type": "string", - "description": "YAML config file to use in BEDGOVCF module" } } }, @@ -300,6 +296,10 @@ "description": "Options specific for the ExomeDepth execution", "default": "", "properties": { + "yamlconfig": { + "type": "string", + "description": "YAML config file to use in BEDGOVCF module" + }, "roi_auto": { "type": "string", "description": "Path to the default ROI (regions of interest) BED file of autosomal (merged) regions to be used for CNV analysis", From fc79ea8dbee77e4051439d49733bed5ad2611193 Mon Sep 17 00:00:00 2001 From: BertGalle Date: Tue, 7 May 2024 15:00:06 +0200 Subject: [PATCH 46/64] Stub fixes --- conf/test.config | 2 +- modules/local/exomedepth/cnv_call/main.nf | 3 ++- modules/local/exomedepth/count/main.nf | 1 + modules/local/exomedepth/merge_cnv/main.nf | 2 +- modules/local/exomedepth/merge_count/main.nf | 2 +- 5 files changed, 6 insertions(+), 4 deletions(-) diff --git a/conf/test.config b/conf/test.config index 60100d6..bdf0ced 100644 --- a/conf/test.config +++ b/conf/test.config @@ -38,7 +38,7 @@ params { // VEP options annotate = null - vep_cache = null + vep_cache = "path" //Change to correct path vep_assembly = "GRCh38" vep_cache_version = 105 species = "homo_sapiens" diff --git a/modules/local/exomedepth/cnv_call/main.nf b/modules/local/exomedepth/cnv_call/main.nf index 8c91ab1..68cacd9 100644 --- a/modules/local/exomedepth/cnv_call/main.nf +++ b/modules/local/exomedepth/cnv_call/main.nf @@ -37,7 +37,8 @@ process CNV_CALL { """ stub: - def prefix = task.ext.prefix ?: "${sample}_CNVs_ExomeDepth_${meta.id}" + def prefix = task.ext.prefix ?: "${sample}_CNVs_ExomeDepth_${meta2.chr}" + def VERSION = '1.1.16' """ touch ${prefix}.txt diff --git a/modules/local/exomedepth/count/main.nf b/modules/local/exomedepth/count/main.nf index 4c1f14e..a8c44da 100644 --- a/modules/local/exomedepth/count/main.nf +++ b/modules/local/exomedepth/count/main.nf @@ -41,6 +41,7 @@ process COUNT { """ stub: + def VERSION = '1.1.16' """ touch ${meta.id}_${meta2.id}.txt diff --git a/modules/local/exomedepth/merge_cnv/main.nf b/modules/local/exomedepth/merge_cnv/main.nf index c4036c1..e14f8d1 100644 --- a/modules/local/exomedepth/merge_cnv/main.nf +++ b/modules/local/exomedepth/merge_cnv/main.nf @@ -8,7 +8,7 @@ process CNV_MERGE { tuple val(meta), path(auto), path(chrx) output: - tuple val(meta), path("${prefix}.txt") + tuple val(meta), path("*.txt") script: prefix = task.ext.prefix ?: "${meta}_CNVs_ExomeDepth" diff --git a/modules/local/exomedepth/merge_count/main.nf b/modules/local/exomedepth/merge_count/main.nf index 0192d5e..5f6151b 100644 --- a/modules/local/exomedepth/merge_count/main.nf +++ b/modules/local/exomedepth/merge_count/main.nf @@ -24,7 +24,7 @@ process COUNT_MERGE { """ stub: - def prefix = task.ext.prefix ?: "${meta.id}_${meta.chr}" + prefix = task.ext.prefix ?: "${meta.id}_${meta.chr}" """ touch ${prefix}.txt """ From dec508dfe3dd8eddf6765c4d8d73c7d0fa7dd335 Mon Sep 17 00:00:00 2001 From: BertGalle Date: Fri, 10 May 2024 13:11:31 +0200 Subject: [PATCH 47/64] Suggestions --- conf/test.config | 2 +- modules/local/exomedepth/cnv_call/environment.yml | 10 ++++++++++ modules/local/exomedepth/cnv_call/main.nf | 1 + modules/local/exomedepth/count/environment.yml | 2 +- .../local/exomedepth/merge_cnv/environment.yml | 7 +++++++ modules/local/exomedepth/merge_cnv/main.nf | 14 ++++++++++++-- .../local/exomedepth/merge_count/environment.yml | 7 +++++++ modules/local/exomedepth/merge_count/main.nf | 15 +++++++++++++-- nextflow_schema.json | 13 +++++++++---- subworkflows/local/exomedepth/main.nf | 8 +++++--- subworkflows/local/vcf_annotation/main.nf | 1 + workflows/exomecnv.nf | 3 +++ 12 files changed, 70 insertions(+), 13 deletions(-) create mode 100644 modules/local/exomedepth/cnv_call/environment.yml create mode 100644 modules/local/exomedepth/merge_cnv/environment.yml create mode 100644 modules/local/exomedepth/merge_count/environment.yml diff --git a/conf/test.config b/conf/test.config index bdf0ced..5020083 100644 --- a/conf/test.config +++ b/conf/test.config @@ -38,7 +38,7 @@ params { // VEP options annotate = null - vep_cache = "path" //Change to correct path + vep_cache = null //Change to correct path vep_assembly = "GRCh38" vep_cache_version = 105 species = "homo_sapiens" diff --git a/modules/local/exomedepth/cnv_call/environment.yml b/modules/local/exomedepth/cnv_call/environment.yml new file mode 100644 index 0000000..01c804a --- /dev/null +++ b/modules/local/exomedepth/cnv_call/environment.yml @@ -0,0 +1,10 @@ +name: exomedepth_cnv_call +channels: + - conda-forge + - bioconda + - defaults + +dependencies: + - r=3.6=r36_1003 + - r-optparse=1.6.4=r36h6115d3f_0 + - r-exomedepth:1.1.16--r43hfb3cda0_3 diff --git a/modules/local/exomedepth/cnv_call/main.nf b/modules/local/exomedepth/cnv_call/main.nf index 68cacd9..b6ce189 100644 --- a/modules/local/exomedepth/cnv_call/main.nf +++ b/modules/local/exomedepth/cnv_call/main.nf @@ -2,6 +2,7 @@ process CNV_CALL { tag "$sample $meta2.chr" label 'process_low' + conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? 'https://depot.galaxyproject.org/singularity/r-exomedepth:1.1.16--r43hfb3cda0_3' : 'biocontainers/r-exomedepth:1.1.16--r43hfb3cda0_3' }" diff --git a/modules/local/exomedepth/count/environment.yml b/modules/local/exomedepth/count/environment.yml index 4cb9d93..f092ff4 100644 --- a/modules/local/exomedepth/count/environment.yml +++ b/modules/local/exomedepth/count/environment.yml @@ -7,4 +7,4 @@ channels: dependencies: - r=3.6=r36_1003 - r-optparse=1.6.4=r36h6115d3f_0 - - r-exomedepth=1.1.12=r36h6786f55_0 + - r-exomedepth:1.1.16--r43hfb3cda0_3 diff --git a/modules/local/exomedepth/merge_cnv/environment.yml b/modules/local/exomedepth/merge_cnv/environment.yml new file mode 100644 index 0000000..63d336e --- /dev/null +++ b/modules/local/exomedepth/merge_cnv/environment.yml @@ -0,0 +1,7 @@ +name: coreutils +channels: + - conda-forge + - bioconda + - defaults +dependencies: + - anaconda::coreutils=9.3 diff --git a/modules/local/exomedepth/merge_cnv/main.nf b/modules/local/exomedepth/merge_cnv/main.nf index e14f8d1..e9f1205 100644 --- a/modules/local/exomedepth/merge_cnv/main.nf +++ b/modules/local/exomedepth/merge_cnv/main.nf @@ -2,19 +2,29 @@ process CNV_MERGE { tag "$meta" + container "quay.io/biocontainers/coreutils:9.3" + conda "${moduleDir}/environment.yml" + publishDir "$params.outdir/exomedepth/cnv_call", mode: 'copy' input: tuple val(meta), path(auto), path(chrx) output: - tuple val(meta), path("*.txt") + tuple val(meta), path("*.txt"), emit:merge + path "versions.yml", emit:versions script: - prefix = task.ext.prefix ?: "${meta}_CNVs_ExomeDepth" + def prefix = task.ext.prefix ?: "${meta}_CNVs_ExomeDepth" """ cp $auto "${prefix}.txt" tail +2 $chrx >> "${prefix}.txt" + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + tail:\$(tail --version | sed '1!d; s/tail//') + cp:\$(cp --version | sed '1!d; s/cp//') + END_VERSIONS """ stub: diff --git a/modules/local/exomedepth/merge_count/environment.yml b/modules/local/exomedepth/merge_count/environment.yml new file mode 100644 index 0000000..63d336e --- /dev/null +++ b/modules/local/exomedepth/merge_count/environment.yml @@ -0,0 +1,7 @@ +name: coreutils +channels: + - conda-forge + - bioconda + - defaults +dependencies: + - anaconda::coreutils=9.3 diff --git a/modules/local/exomedepth/merge_count/main.nf b/modules/local/exomedepth/merge_count/main.nf index 5f6151b..e3449fe 100644 --- a/modules/local/exomedepth/merge_count/main.nf +++ b/modules/local/exomedepth/merge_count/main.nf @@ -2,16 +2,20 @@ process COUNT_MERGE { tag "$meta.id $meta.chr" + container "quay.io/biocontainers/coreutils:9.3" + conda "${moduleDir}/environment.yml" + publishDir "$params.outdir/exomedepth/counts", mode: 'copy' input: tuple val(meta), path(files) output: - tuple val(meta), path("${prefix}.txt") + tuple val(meta), path("*.txt"), emit:merge + path "versions.yml", emit:versions script: - prefix = task.ext.prefix ?: "${meta.id}_${meta.chr}" + def prefix = task.ext.prefix ?: "${meta.id}_${meta.chr}" """ for file in $files; do if [ -f ${prefix}.txt ]; then @@ -21,6 +25,13 @@ process COUNT_MERGE { cp \$file ${prefix}.txt fi done + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + paste:\$(paste --version | sed '1!d; s/paste//') + mv:\$(mv --version | sed '1!d; s/mv//') + cp:\$(cp --version | sed '1!d; s/cp//') + END_VERSIONS """ stub: diff --git a/nextflow_schema.json b/nextflow_schema.json index ed4258c..49c0d73 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -298,21 +298,25 @@ "properties": { "yamlconfig": { "type": "string", - "description": "YAML config file to use in BEDGOVCF module" + "description": "YAML config file to use in BEDGOVCF module", + "exists": true, + "format": "file-path" }, "roi_auto": { "type": "string", "description": "Path to the default ROI (regions of interest) BED file of autosomal (merged) regions to be used for CNV analysis", "format": "file-path", "pattern": "^\\S+\\.bed$", - "mimetype": "text/plain" + "mimetype": "text/plain", + "exists": true }, "roi_chrx": { "type": "string", "description": "Path to the default ROI (regions of interest) BED file of chrX (merged) regions to be used for CNV analysis", "format": "file-path", "pattern": "^\\S+\\.bed$", - "mimetype": "text/plain" + "mimetype": "text/plain", + "exists": true } }, "required": ["roi_auto", "roi_chrx"] @@ -335,11 +339,12 @@ }, "vep_cache": { "type": "string", + "format": "directory-path", "description": "Path to the VEP cache" }, "annotate": { "type": "string", - "description": "Path to VCF file to run VEP on" + "description": "Path to samplesheet with VCF files to run VEP on" }, "vep_assembly": { "type": "string", diff --git a/subworkflows/local/exomedepth/main.nf b/subworkflows/local/exomedepth/main.nf index 3345126..cfebd74 100644 --- a/subworkflows/local/exomedepth/main.nf +++ b/subworkflows/local/exomedepth/main.nf @@ -80,10 +80,11 @@ workflow EXOMEDEPTH { COUNT_MERGE_AUTO ( grouped_counts_auto ) + ch_versions = ch_versions.mix(COUNT_MERGE_AUTO.out.versions) //MODULE: Autosomal CNV call per sample (file for each sample) - cnv_auto_ch = COUNT_MERGE_AUTO.out + cnv_auto_ch = COUNT_MERGE_AUTO.out.merge .map { meta, txt -> [meta, meta.sam, txt] } @@ -119,7 +120,7 @@ workflow EXOMEDEPTH { //MODULE: ChrX CNV call per sample (file for each sample) - cnv_chrx_ch = COUNT_MERGE_X.out + cnv_chrx_ch = COUNT_MERGE_X.out.merge .map { meta, txt -> [meta, meta.sam, txt] } @@ -138,10 +139,11 @@ workflow EXOMEDEPTH { } CNV_MERGE(cnv_merge_ch) + ch_versions = ch_versions.mix(CNV_MERGE.out.versions) //MODULE: Convert file to VCF according to a YAML config - bedgovcf_input = CNV_MERGE.out + bedgovcf_input = CNV_MERGE.out.merge .map{ meta, bed -> def new_meta = [id:meta] [new_meta, bed, params.yamlconfig] diff --git a/subworkflows/local/vcf_annotation/main.nf b/subworkflows/local/vcf_annotation/main.nf index ccb0e5c..45fe813 100644 --- a/subworkflows/local/vcf_annotation/main.nf +++ b/subworkflows/local/vcf_annotation/main.nf @@ -36,4 +36,5 @@ workflow ENSEMBLVEP { emit: vcfs = VEP.out.vcf + versions = VEP.out.versions } diff --git a/workflows/exomecnv.nf b/workflows/exomecnv.nf index 5308536..17a8933 100644 --- a/workflows/exomecnv.nf +++ b/workflows/exomecnv.nf @@ -53,8 +53,10 @@ workflow EXOMECNV { [meta, path, ch_empty]} ENSEMBLVEP ( ch_exomedepth_vcf, ch_fasta, ch_vep_cache ) } + ch_versions = ch_versions.mix(ENSEMBLVEP.out.versions) } + // EnsemblVEP on VCF input file else { @@ -68,6 +70,7 @@ workflow EXOMECNV { ENSEMBLVEP ( ch_vcf, ch_fasta, ch_vep_cache ) + ch_versions = ENSEMBLVEP.out.versions } // Collate and save software versions From 08f74fcc19096c497087666c9d64b3f9864b622d Mon Sep 17 00:00:00 2001 From: BertGalle Date: Fri, 10 May 2024 14:19:20 +0200 Subject: [PATCH 48/64] Config --- conf/test.config | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/conf/test.config b/conf/test.config index 5020083..bdf0ced 100644 --- a/conf/test.config +++ b/conf/test.config @@ -38,7 +38,7 @@ params { // VEP options annotate = null - vep_cache = null //Change to correct path + vep_cache = "path" //Change to correct path vep_assembly = "GRCh38" vep_cache_version = 105 species = "homo_sapiens" From 0ea8d2d907180f70ddd061f9bf9072e3eb3ea9e7 Mon Sep 17 00:00:00 2001 From: BertGalle Date: Fri, 10 May 2024 15:17:11 +0200 Subject: [PATCH 49/64] Fix --- modules/local/exomedepth/merge_cnv/main.nf | 6 ++++++ modules/local/exomedepth/merge_count/main.nf | 7 +++++++ 2 files changed, 13 insertions(+) diff --git a/modules/local/exomedepth/merge_cnv/main.nf b/modules/local/exomedepth/merge_cnv/main.nf index e9f1205..2387935 100644 --- a/modules/local/exomedepth/merge_cnv/main.nf +++ b/modules/local/exomedepth/merge_cnv/main.nf @@ -31,5 +31,11 @@ process CNV_MERGE { def prefix = task.ext.prefix ?: "${meta}_CNVs_ExomeDepth" """ touch ${prefix}.txt + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + tail:\$(tail --version | sed '1!d; s/tail//') + cp:\$(cp --version | sed '1!d; s/cp//') + END_VERSIONS """ } diff --git a/modules/local/exomedepth/merge_count/main.nf b/modules/local/exomedepth/merge_count/main.nf index e3449fe..43e05c5 100644 --- a/modules/local/exomedepth/merge_count/main.nf +++ b/modules/local/exomedepth/merge_count/main.nf @@ -38,5 +38,12 @@ process COUNT_MERGE { prefix = task.ext.prefix ?: "${meta.id}_${meta.chr}" """ touch ${prefix}.txt + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + paste:\$(paste --version | sed '1!d; s/paste//') + mv:\$(mv --version | sed '1!d; s/mv//') + cp:\$(cp --version | sed '1!d; s/cp//') + END_VERSIONS """ } From 0ca24e68230e29f3468c7f4b6bcf1958eeadee01 Mon Sep 17 00:00:00 2001 From: BertGalle Date: Fri, 10 May 2024 15:50:00 +0200 Subject: [PATCH 50/64] Module config --- conf/modules.config | 20 -------------------- 1 file changed, 20 deletions(-) diff --git a/conf/modules.config b/conf/modules.config index 4e15f97..9a622fd 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -18,18 +18,6 @@ process { saveAs: { filename -> filename.equals('versions.yml') ? null : filename } ] - withName: FASTQC { - ext.args = '--quiet' - } - - withName: CUSTOM_DUMPSOFTWAREVERSIONS { - publishDir = [ - path: { "${params.outdir}/pipeline_info" }, - mode: params.publish_dir_mode, - pattern: '*_versions.yml' - ] - } - withName: 'MULTIQC' { ext.args = { params.multiqc_title ? "--title \"$params.multiqc_title\"" : '' } publishDir = [ @@ -39,14 +27,6 @@ process { ] } - withName: 'CRAM_TO_BAM' { - publishDir = [ - path: { "${params.outdir}/bam" }, - mode: params.publish_dir_mode, - saveAs: { filename -> filename.equals('versions.yml') ? null : filename } - ] - } - withName: 'BEDGOVCF' { ext.args = { "--skip 1" } } From 11b5bed63071d7404093441ea3da764ec90db904 Mon Sep 17 00:00:00 2001 From: BertGalle Date: Mon, 13 May 2024 09:10:45 +0200 Subject: [PATCH 51/64] Removed modules in config --- README.md | 1 + conf/base.config | 4 +--- docs/images/nfcore-exomecnv_logo.png | Bin 0 -> 78871 bytes workflows/exomecnv.nf | 3 +-- 4 files changed, 3 insertions(+), 5 deletions(-) create mode 100644 docs/images/nfcore-exomecnv_logo.png diff --git a/README.md b/README.md index 42588c8..9bcf89e 100644 --- a/README.md +++ b/README.md @@ -1,3 +1,4 @@ +![nf-core/exomecnv](docs/images/nfcore-exomecnv_logo.png) [![GitHub Actions CI Status](https://github.com/nf-cmgg/exomecnv/actions/workflows/ci.yml/badge.svg)](https://github.com/nf-cmgg/exomecnv/actions/workflows/ci.yml) [![GitHub Actions Linting Status](https://github.com/nf-cmgg/exomecnv/actions/workflows/linting.yml/badge.svg)](https://github.com/nf-cmgg/exomecnv/actions/workflows/linting.yml)[![Cite with Zenodo](http://img.shields.io/badge/DOI-10.5281/zenodo.XXXXXXX-1073c8?labelColor=000000)](https://doi.org/10.5281/zenodo.XXXXXXX) [![nf-test](https://img.shields.io/badge/unit_tests-nf--test-337ab7.svg)](https://www.nf-test.com) diff --git a/conf/base.config b/conf/base.config index bbae968..4e6afb3 100644 --- a/conf/base.config +++ b/conf/base.config @@ -59,7 +59,5 @@ process { errorStrategy = 'retry' maxRetries = 2 } - withName:CUSTOM_DUMPSOFTWAREVERSIONS { - cache = false - } + } diff --git a/docs/images/nfcore-exomecnv_logo.png b/docs/images/nfcore-exomecnv_logo.png new file mode 100644 index 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b/workflows/exomecnv.nf @@ -52,10 +52,9 @@ workflow EXOMECNV { .map { meta, path -> [meta, path, ch_empty]} ENSEMBLVEP ( ch_exomedepth_vcf, ch_fasta, ch_vep_cache ) - } ch_versions = ch_versions.mix(ENSEMBLVEP.out.versions) } - + } // EnsemblVEP on VCF input file From ed2653a333c4fac215a1898ae0fa5e7096cdad5c Mon Sep 17 00:00:00 2001 From: BertGalle Date: Mon, 13 May 2024 15:48:02 +0200 Subject: [PATCH 52/64] Changed the way --annotate works --- assets/schema_input.json | 7 +++++++ conf/test.config | 2 +- modules/local/exomedepth/merge_cnv/main.nf | 12 +++++++----- modules/local/exomedepth/merge_count/main.nf | 18 +++++++++++------- nextflow_schema.json | 4 ++-- subworkflows/local/exomedepth/main.nf | 4 +++- workflows/exomecnv.nf | 10 ++++------ 7 files changed, 35 insertions(+), 22 deletions(-) diff --git a/assets/schema_input.json b/assets/schema_input.json index d4ce515..022b3b9 100644 --- a/assets/schema_input.json +++ b/assets/schema_input.json @@ -38,6 +38,13 @@ "errorMessage": "index of CRAM or BAM file must be provided, cannot contain spaces and must have extension '.crai' or '.bai' or '.csi'", "format": "file-path", "exists": true + }, + "vcf": { + "type": "string", + "pattern": "^\\S+\\.vcf$", + "errorMessage": "VCF is optional but must be provided when only annotating", + "format": "file-path", + "exists": true } }, "required": ["sample", "pool", "family", "cram"] diff --git a/conf/test.config b/conf/test.config index bdf0ced..6e89afb 100644 --- a/conf/test.config +++ b/conf/test.config @@ -37,7 +37,7 @@ params { // VEP options - annotate = null + annotate = false vep_cache = "path" //Change to correct path vep_assembly = "GRCh38" vep_cache_version = 105 diff --git a/modules/local/exomedepth/merge_cnv/main.nf b/modules/local/exomedepth/merge_cnv/main.nf index 2387935..d63b4f2 100644 --- a/modules/local/exomedepth/merge_cnv/main.nf +++ b/modules/local/exomedepth/merge_cnv/main.nf @@ -2,7 +2,9 @@ process CNV_MERGE { tag "$meta" - container "quay.io/biocontainers/coreutils:9.3" + container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? + 'https://depot.galaxyproject.org/singularity/coreutils:9.3' : + 'biocontainers/coreutils:9.3' }" conda "${moduleDir}/environment.yml" publishDir "$params.outdir/exomedepth/cnv_call", mode: 'copy' @@ -22,8 +24,8 @@ process CNV_MERGE { cat <<-END_VERSIONS > versions.yml "${task.process}": - tail:\$(tail --version | sed '1!d; s/tail//') - cp:\$(cp --version | sed '1!d; s/cp//') + tail: \$(tail --version | sed '1!d; s/tail (GNU coreutils) //') + cp: \$(cp --version | sed '1!d; s/cp (GNU coreutils) //') END_VERSIONS """ @@ -34,8 +36,8 @@ process CNV_MERGE { cat <<-END_VERSIONS > versions.yml "${task.process}": - tail:\$(tail --version | sed '1!d; s/tail//') - cp:\$(cp --version | sed '1!d; s/cp//') + tail: \$(tail --version | sed '1!d; s/tail (GNU coreutils) //') + cp: \$(cp --version | sed '1!d; s/cp (GNU coreutils) //') END_VERSIONS """ } diff --git a/modules/local/exomedepth/merge_count/main.nf b/modules/local/exomedepth/merge_count/main.nf index 43e05c5..e4613bb 100644 --- a/modules/local/exomedepth/merge_count/main.nf +++ b/modules/local/exomedepth/merge_count/main.nf @@ -2,7 +2,9 @@ process COUNT_MERGE { tag "$meta.id $meta.chr" - container "quay.io/biocontainers/coreutils:9.3" + container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? + 'https://depot.galaxyproject.org/singularity/coreutils:9.3' : + 'biocontainers/coreutils:9.3' }" conda "${moduleDir}/environment.yml" publishDir "$params.outdir/exomedepth/counts", mode: 'copy' @@ -28,9 +30,10 @@ process COUNT_MERGE { cat <<-END_VERSIONS > versions.yml "${task.process}": - paste:\$(paste --version | sed '1!d; s/paste//') - mv:\$(mv --version | sed '1!d; s/mv//') - cp:\$(cp --version | sed '1!d; s/cp//') + paste: \$(paste --version | sed '1!d; s/paste (GNU coreutils) //') + mv: \$(mv --version | sed '1!d; s/mv (GNU coreutils) //') + cp: \$(cp --version | sed '1!d; s/cp (GNU coreutils) //') + awk: \$(awk -version 2>&1 | sed '1!d; s/(.*//') END_VERSIONS """ @@ -41,9 +44,10 @@ process COUNT_MERGE { cat <<-END_VERSIONS > versions.yml "${task.process}": - paste:\$(paste --version | sed '1!d; s/paste//') - mv:\$(mv --version | sed '1!d; s/mv//') - cp:\$(cp --version | sed '1!d; s/cp//') + paste: \$(paste --version | sed '1!d; s/paste (GNU coreutils) //') + mv: \$(mv --version | sed '1!d; s/mv (GNU coreutils) //') + cp: \$(cp --version | sed '1!d; s/cp (GNU coreutils) //') + awk: \$(awk -version 2>&1 | sed '1!d; s/(.*//') END_VERSIONS """ } diff --git a/nextflow_schema.json b/nextflow_schema.json index 49c0d73..886e77a 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -343,8 +343,8 @@ "description": "Path to the VEP cache" }, "annotate": { - "type": "string", - "description": "Path to samplesheet with VCF files to run VEP on" + "type": "boolean", + "description": "Option to annotate on vcf input" }, "vep_assembly": { "type": "string", diff --git a/subworkflows/local/exomedepth/main.nf b/subworkflows/local/exomedepth/main.nf index cfebd74..51560b4 100644 --- a/subworkflows/local/exomedepth/main.nf +++ b/subworkflows/local/exomedepth/main.nf @@ -31,7 +31,9 @@ workflow EXOMEDEPTH { // Importing and convert the input files passed through the parameters to channels // Branch into CRAM and BAM files - ch_samplesheet.branch { meta, cram, crai -> + ch_samplesheet.map { meta, cram, crai, vcf -> + [meta, cram, crai]} + .branch { meta, cram, crai -> CRAM: cram.extension == "cram" BAM: cram.extension == "bam" } diff --git a/workflows/exomecnv.nf b/workflows/exomecnv.nf index 4cf031f..b229fa9 100644 --- a/workflows/exomecnv.nf +++ b/workflows/exomecnv.nf @@ -60,14 +60,12 @@ workflow EXOMECNV { else { ch_empty = [] - ch_vcf = Channel.fromPath(params.annotate) - .splitCsv( header:true) - .map { row -> - [[id:row.sample], row.vcf, ch_empty]} - .collect() + ch_vcf = ch_samplesheet + .map { meta,bam,bai,vcf -> + [[id:meta.id], vcf, ch_empty]} ENSEMBLVEP ( - ch_vcf, ch_fasta, ch_vep_cache + ch_vcf, ch_fasta, ch_vep_cache ) ch_versions = ENSEMBLVEP.out.versions } From fb5fed56ae2a5cf31f69ebca003e93504b673681 Mon Sep 17 00:00:00 2001 From: BertGalle Date: Tue, 14 May 2024 09:20:46 +0200 Subject: [PATCH 53/64] Added index files for VCF --- assets/schema_input.json | 9 +- modules.json | 5 + modules/nf-core/tabix/tabix/environment.yml | 8 + modules/nf-core/tabix/tabix/main.nf | 42 ++++ modules/nf-core/tabix/tabix/meta.yml | 49 ++++ .../nf-core/tabix/tabix/tests/main.nf.test | 142 ++++++++++++ .../tabix/tabix/tests/main.nf.test.snap | 217 ++++++++++++++++++ .../tabix/tabix/tests/tabix_bed.config | 5 + .../tabix/tabix/tests/tabix_gff.config | 5 + .../tabix/tabix/tests/tabix_vcf_csi.config | 5 + .../tabix/tabix/tests/tabix_vcf_tbi.config | 5 + modules/nf-core/tabix/tabix/tests/tags.yml | 2 + subworkflows/local/exomedepth/main.nf | 2 +- workflows/exomecnv.nf | 21 +- 14 files changed, 506 insertions(+), 11 deletions(-) create mode 100644 modules/nf-core/tabix/tabix/environment.yml create mode 100644 modules/nf-core/tabix/tabix/main.nf create mode 100644 modules/nf-core/tabix/tabix/meta.yml create mode 100644 modules/nf-core/tabix/tabix/tests/main.nf.test create mode 100644 modules/nf-core/tabix/tabix/tests/main.nf.test.snap create mode 100644 modules/nf-core/tabix/tabix/tests/tabix_bed.config create mode 100644 modules/nf-core/tabix/tabix/tests/tabix_gff.config create mode 100644 modules/nf-core/tabix/tabix/tests/tabix_vcf_csi.config create mode 100644 modules/nf-core/tabix/tabix/tests/tabix_vcf_tbi.config create mode 100644 modules/nf-core/tabix/tabix/tests/tags.yml diff --git a/assets/schema_input.json b/assets/schema_input.json index 022b3b9..48c3cd8 100644 --- a/assets/schema_input.json +++ b/assets/schema_input.json @@ -41,10 +41,17 @@ }, "vcf": { "type": "string", - "pattern": "^\\S+\\.vcf$", + "pattern": "^\\S+\\.(vcf|vcf.gz)$", "errorMessage": "VCF is optional but must be provided when only annotating", "format": "file-path", "exists": true + }, + "tbi": { + "type": "string", + "pattern": "^\\S+\\.(vcf.gz.tbi)$", + "errorMessage": "index of VCF file is optional but must be provided when only annotating", + "format": "file-path", + "exists": true } }, "required": ["sample", "pool", "family", "cram"] diff --git a/modules.json b/modules.json index abd3e44..126ef39 100644 --- a/modules.json +++ b/modules.json @@ -24,6 +24,11 @@ "branch": "master", "git_sha": "b42fec6f7c6e5d0716685cabb825ef6bf6e386b5", "installed_by": ["modules"] + }, + "tabix/tabix": { + "branch": "master", + "git_sha": "9502adb23c0b97ed8e616bbbdfa73b4585aec9a1", + "installed_by": ["modules"] } } }, diff --git a/modules/nf-core/tabix/tabix/environment.yml b/modules/nf-core/tabix/tabix/environment.yml new file mode 100644 index 0000000..76b45e1 --- /dev/null +++ b/modules/nf-core/tabix/tabix/environment.yml @@ -0,0 +1,8 @@ +name: tabix_tabix +channels: + - conda-forge + - bioconda + - defaults +dependencies: + - bioconda::tabix=1.11 + - bioconda::htslib=1.19.1 diff --git a/modules/nf-core/tabix/tabix/main.nf b/modules/nf-core/tabix/tabix/main.nf new file mode 100644 index 0000000..1737141 --- /dev/null +++ b/modules/nf-core/tabix/tabix/main.nf @@ -0,0 +1,42 @@ +process TABIX_TABIX { + tag "$meta.id" + label 'process_single' + + conda "${moduleDir}/environment.yml" + container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? + 'https://depot.galaxyproject.org/singularity/htslib:1.19.1--h81da01d_1' : + 'biocontainers/htslib:1.19.1--h81da01d_1' }" + + input: + tuple val(meta), path(tab) + + output: + tuple val(meta), path("*.tbi"), optional:true, emit: tbi + tuple val(meta), path("*.csi"), optional:true, emit: csi + path "versions.yml" , emit: versions + + when: + task.ext.when == null || task.ext.when + + script: + def args = task.ext.args ?: '' + """ + tabix $args $tab + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + tabix: \$(echo \$(tabix -h 2>&1) | sed 's/^.*Version: //; s/ .*\$//') + END_VERSIONS + """ + + stub: + """ + touch ${tab}.tbi + touch ${tab}.csi + cat <<-END_VERSIONS > versions.yml + + "${task.process}": + tabix: \$(echo \$(tabix -h 2>&1) | sed 's/^.*Version: //; s/ .*\$//') + END_VERSIONS + """ +} diff --git a/modules/nf-core/tabix/tabix/meta.yml b/modules/nf-core/tabix/tabix/meta.yml new file mode 100644 index 0000000..ae5b4f4 --- /dev/null +++ b/modules/nf-core/tabix/tabix/meta.yml @@ -0,0 +1,49 @@ +name: tabix_tabix +description: create tabix index from a sorted bgzip tab-delimited genome file +keywords: + - index + - tabix + - vcf +tools: + - tabix: + description: Generic indexer for TAB-delimited genome position files. + homepage: https://www.htslib.org/doc/tabix.html + documentation: https://www.htslib.org/doc/tabix.1.html + doi: 10.1093/bioinformatics/btq671 + licence: ["MIT"] +input: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - tab: + type: file + description: TAB-delimited genome position file compressed with bgzip + pattern: "*.{bed.gz,gff.gz,sam.gz,vcf.gz}" +output: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - tbi: + type: file + description: tabix index file + pattern: "*.{tbi}" + - csi: + type: file + description: coordinate sorted index file + pattern: "*.{csi}" + - versions: + type: file + description: File containing software versions + pattern: "versions.yml" +authors: + - "@joseespinosa" + - "@drpatelh" + - "@maxulysse" +maintainers: + - "@joseespinosa" + - "@drpatelh" + - "@maxulysse" diff --git a/modules/nf-core/tabix/tabix/tests/main.nf.test b/modules/nf-core/tabix/tabix/tests/main.nf.test new file mode 100644 index 0000000..3a150c7 --- /dev/null +++ b/modules/nf-core/tabix/tabix/tests/main.nf.test @@ -0,0 +1,142 @@ +nextflow_process { + + name "Test Process TABIX_TABIX" + script "modules/nf-core/tabix/tabix/main.nf" + process "TABIX_TABIX" + + tag "modules" + tag "modules_nfcore" + tag "tabix" + tag "tabix/tabix" + + test("sarscov2_bedgz_tbi") { + config "./tabix_bed.config" + when { + process { + """ + input[0] = [ + [ id:'tbi_bed' ], + [ file(params.test_data['sarscov2']['genome']['test_bed_gz'], checkIfExists: true) ] + ] + """ + } + } + + then { + assertAll ( + { assert process.success }, + { assert snapshot(process.out).match() }, + { assert snapshot( + file(process.out.tbi[0][1]).name + ).match("tbi_bed") + } + ) + } + } + + test("sarscov2_gff_tbi") { + config "./tabix_gff.config" + when { + process { + """ + input[0] = [ + [ id:'tbi_gff' ], + [ file(params.test_data['sarscov2']['genome']['genome_gff3_gz'], checkIfExists: true) ] + ] + """ + } + } + + then { + assertAll ( + { assert process.success }, + { assert snapshot(process.out).match() }, + { assert snapshot( + file(process.out.tbi[0][1]).name + ).match("tbi_gff") + } + ) + } + + } + + test("sarscov2_vcf_tbi") { + config "./tabix_vcf_tbi.config" + when { + process { + """ + input[0] = [ + [ id:'tbi_vcf' ], + [ file(params.test_data['sarscov2']['illumina']['test_vcf_gz'], checkIfExists: true) ] + ] + """ + } + } + + then { + assertAll ( + { assert process.success }, + { assert snapshot(process.out).match() }, + { assert snapshot( + file(process.out.tbi[0][1]).name + ).match("tbi_vcf") + } + ) + } + + } + + test("sarscov2_vcf_csi") { + config "./tabix_vcf_csi.config" + when { + process { + """ + input[0] = [ + [ id:'vcf_csi' ], + [ file(params.test_data['sarscov2']['illumina']['test_vcf_gz'], checkIfExists: true) ] + ] + """ + } + } + + then { + assertAll ( + { assert process.success }, + { assert snapshot(process.out).match() }, + { assert snapshot( + file(process.out.csi[0][1]).name + ).match("vcf_csi") + } + ) + } + + } + + test("sarscov2_vcf_csi_stub") { + config "./tabix_vcf_csi.config" + options "-stub" + when { + process { + """ + input[0] = [ + [ id:'vcf_csi_stub' ], + [ file(params.test_data['sarscov2']['illumina']['test_vcf_gz'], checkIfExists: true) ] + ] + """ + } + } + + then { + assertAll ( + { assert process.success }, + { assert snapshot(process.out).match() }, + { assert snapshot( + file(process.out.csi[0][1]).name + ).match("vcf_csi_stub") + } + ) + } + + } + +} diff --git a/modules/nf-core/tabix/tabix/tests/main.nf.test.snap b/modules/nf-core/tabix/tabix/tests/main.nf.test.snap new file mode 100644 index 0000000..034e38b --- /dev/null +++ b/modules/nf-core/tabix/tabix/tests/main.nf.test.snap @@ -0,0 +1,217 @@ +{ + "vcf_csi_stub": { + "content": [ + "test.vcf.gz.csi" + ], + "timestamp": "2024-03-04T14:51:59.788002" + }, + "tbi_gff": { + "content": [ + "genome.gff3.gz.tbi" + ], + "timestamp": "2024-02-19T14:53:37.420216" + }, + "sarscov2_gff_tbi": { + "content": [ + { + "0": [ + [ + { + "id": "tbi_gff" + }, + "genome.gff3.gz.tbi:md5,53fc683fd217aae47ef10d23c52a9178" + ] + ], + "1": [ + + ], + "2": [ + "versions.yml:md5,f4feeda7fdd4b567102f7f8e5d7037a3" + ], + "csi": [ + + ], + "tbi": [ + [ + { + "id": "tbi_gff" + }, + "genome.gff3.gz.tbi:md5,53fc683fd217aae47ef10d23c52a9178" + ] + ], + "versions": [ + "versions.yml:md5,f4feeda7fdd4b567102f7f8e5d7037a3" + ] + } + ], + "timestamp": "2024-02-19T14:53:37.388157" + }, + "sarscov2_bedgz_tbi": { + "content": [ + { + "0": [ + [ + { + "id": "tbi_bed" + }, + "test.bed.gz.tbi:md5,0f17d85e7f0a042b2aa367b70df224f8" + ] + ], + "1": [ + + ], + "2": [ + "versions.yml:md5,f4feeda7fdd4b567102f7f8e5d7037a3" + ], + "csi": [ + + ], + "tbi": [ + [ + { + "id": "tbi_bed" + }, + "test.bed.gz.tbi:md5,0f17d85e7f0a042b2aa367b70df224f8" + ] + ], + "versions": [ + "versions.yml:md5,f4feeda7fdd4b567102f7f8e5d7037a3" + ] + } + ], + "timestamp": "2024-02-19T14:53:28.879408" + }, + "tbi_vcf": { + "content": [ + "test.vcf.gz.tbi" + ], + "timestamp": "2024-02-19T14:53:46.402522" + }, + "vcf_csi": { + "content": [ + "test.vcf.gz.csi" + ], + "timestamp": "2024-02-19T14:53:54.921189" + }, + "sarscov2_vcf_tbi": { + "content": [ + { + "0": [ + [ + { + "id": "tbi_vcf" + }, + "test.vcf.gz.tbi:md5,897f3f378a811b90e6dee56ce08d2bcf" + ] + ], + "1": [ + + ], + "2": [ + "versions.yml:md5,f4feeda7fdd4b567102f7f8e5d7037a3" + ], + "csi": [ + + ], + "tbi": [ + [ + { + "id": "tbi_vcf" + }, + "test.vcf.gz.tbi:md5,897f3f378a811b90e6dee56ce08d2bcf" + ] + ], + "versions": [ + "versions.yml:md5,f4feeda7fdd4b567102f7f8e5d7037a3" + ] + } + ], + "timestamp": "2024-02-19T14:53:46.370358" + }, + "sarscov2_vcf_csi_stub": { + "content": [ + { + "0": [ + [ + { + "id": "vcf_csi_stub" + }, + "test.vcf.gz.tbi:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "1": [ + [ + { + "id": "vcf_csi_stub" + }, + "test.vcf.gz.csi:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "2": [ + "versions.yml:md5,3d45df6d80883bad358631069a2940fd" + ], + "csi": [ + [ + { + "id": "vcf_csi_stub" + }, + "test.vcf.gz.csi:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "tbi": [ + [ + { + "id": "vcf_csi_stub" + }, + "test.vcf.gz.tbi:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "versions": [ + "versions.yml:md5,3d45df6d80883bad358631069a2940fd" + ] + } + ], + "timestamp": "2024-03-04T14:51:59.766184" + }, + "sarscov2_vcf_csi": { + "content": [ + { + "0": [ + + ], + "1": [ + [ + { + "id": "vcf_csi" + }, + "test.vcf.gz.csi:md5,0731ad6f40104d2bbb1a2cc478ef8f03" + ] + ], + "2": [ + "versions.yml:md5,f4feeda7fdd4b567102f7f8e5d7037a3" + ], + "csi": [ + [ + { + "id": "vcf_csi" + }, + "test.vcf.gz.csi:md5,0731ad6f40104d2bbb1a2cc478ef8f03" + ] + ], + "tbi": [ + + ], + "versions": [ + "versions.yml:md5,f4feeda7fdd4b567102f7f8e5d7037a3" + ] + } + ], + "timestamp": "2024-02-19T14:53:54.886876" + }, + "tbi_bed": { + "content": [ + "test.bed.gz.tbi" + ], + "timestamp": "2024-02-19T14:53:28.947628" + } +} \ No newline at end of file diff --git a/modules/nf-core/tabix/tabix/tests/tabix_bed.config b/modules/nf-core/tabix/tabix/tests/tabix_bed.config new file mode 100644 index 0000000..7ff0590 --- /dev/null +++ b/modules/nf-core/tabix/tabix/tests/tabix_bed.config @@ -0,0 +1,5 @@ +process { + withName: TABIX_TABIX { + ext.args = '-p bed' + } +} \ No newline at end of file diff --git a/modules/nf-core/tabix/tabix/tests/tabix_gff.config b/modules/nf-core/tabix/tabix/tests/tabix_gff.config new file mode 100644 index 0000000..20c0a1e --- /dev/null +++ b/modules/nf-core/tabix/tabix/tests/tabix_gff.config @@ -0,0 +1,5 @@ +process { + withName: TABIX_TABIX { + ext.args = '-p gff' + } +} \ No newline at end of file diff --git a/modules/nf-core/tabix/tabix/tests/tabix_vcf_csi.config b/modules/nf-core/tabix/tabix/tests/tabix_vcf_csi.config new file mode 100644 index 0000000..eb4f2d7 --- /dev/null +++ b/modules/nf-core/tabix/tabix/tests/tabix_vcf_csi.config @@ -0,0 +1,5 @@ +process { + withName: TABIX_TABIX { + ext.args = '-p vcf --csi' + } +} diff --git a/modules/nf-core/tabix/tabix/tests/tabix_vcf_tbi.config b/modules/nf-core/tabix/tabix/tests/tabix_vcf_tbi.config new file mode 100644 index 0000000..2774c8a --- /dev/null +++ b/modules/nf-core/tabix/tabix/tests/tabix_vcf_tbi.config @@ -0,0 +1,5 @@ +process { + withName: TABIX_TABIX { + ext.args = '-p vcf' + } +} \ No newline at end of file diff --git a/modules/nf-core/tabix/tabix/tests/tags.yml b/modules/nf-core/tabix/tabix/tests/tags.yml new file mode 100644 index 0000000..6eda065 --- /dev/null +++ b/modules/nf-core/tabix/tabix/tests/tags.yml @@ -0,0 +1,2 @@ +tabix/tabix: + - "modules/nf-core/tabix/tabix/**" diff --git a/subworkflows/local/exomedepth/main.nf b/subworkflows/local/exomedepth/main.nf index 51560b4..4e24e86 100644 --- a/subworkflows/local/exomedepth/main.nf +++ b/subworkflows/local/exomedepth/main.nf @@ -31,7 +31,7 @@ workflow EXOMEDEPTH { // Importing and convert the input files passed through the parameters to channels // Branch into CRAM and BAM files - ch_samplesheet.map { meta, cram, crai, vcf -> + ch_samplesheet.map { meta, cram, crai, vcf, tbi -> [meta, cram, crai]} .branch { meta, cram, crai -> CRAM: cram.extension == "cram" diff --git a/workflows/exomecnv.nf b/workflows/exomecnv.nf index b229fa9..80057dd 100644 --- a/workflows/exomecnv.nf +++ b/workflows/exomecnv.nf @@ -11,8 +11,9 @@ include { softwareVersionsToYAML } from '../subworkflows/nf-core/utils_nfcore_pi include { methodsDescriptionText } from '../subworkflows/local/utils_nfcore_exomecnv_pipeline' // local -include { EXOMEDEPTH } from '../subworkflows/local/exomedepth/main' -include { ENSEMBLVEP } from '../subworkflows/local/vcf_annotation/main' +include { EXOMEDEPTH } from '../subworkflows/local/exomedepth/main' +include { ENSEMBLVEP } from '../subworkflows/local/vcf_annotation/main' +include { TABIX_TABIX as TABIX } from '../modules/nf-core/tabix/tabix/main' /* ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ @@ -46,11 +47,14 @@ workflow EXOMECNV { EXOMEDEPTH (ch_samplesheet) ch_versions = EXOMEDEPTH.out.versions - // EnsemblVEP after ExomeDepth - ch_empty = [] + // Index files for VCF + + TABIX ( EXOMEDEPTH.out.vcf ) ch_exomedepth_vcf = EXOMEDEPTH.out.vcf - .map { meta, path -> - [meta, path, ch_empty]} + .join(TABIX.out.tbi) + + // EnsemblVEP after ExomeDepth + ENSEMBLVEP ( ch_exomedepth_vcf, ch_fasta, ch_vep_cache ) ch_versions = ch_versions.mix(ENSEMBLVEP.out.versions) } @@ -59,10 +63,9 @@ workflow EXOMECNV { // EnsemblVEP on VCF input file else { - ch_empty = [] ch_vcf = ch_samplesheet - .map { meta,bam,bai,vcf -> - [[id:meta.id], vcf, ch_empty]} + .map { meta,bam,bai,vcf,tbi -> + [[id:meta.id], vcf, tbi]} ENSEMBLVEP ( ch_vcf, ch_fasta, ch_vep_cache From 8961d1bd06331f2df752fce14514e90df1c04df1 Mon Sep 17 00:00:00 2001 From: BertGalle Date: Mon, 3 Jun 2024 11:37:54 +0200 Subject: [PATCH 54/64] Suggested changes + readme --- CITATIONS.md | 7 ++- Exomedepth2.png | Bin 0 -> 137464 bytes README.md | 67 +++++++++++----------- conf/modules.config | 12 ++++ docs/images/nfcore-exomecnv_logo.png | Bin 78871 -> 89499 bytes nextflow_schema.json | 3 +- subworkflows/local/exomedepth/main.nf | 4 +- subworkflows/local/vcf_annotation/main.nf | 2 +- workflows/exomecnv.nf | 33 +++++++---- 9 files changed, 75 insertions(+), 53 deletions(-) create mode 100644 Exomedepth2.png diff --git a/CITATIONS.md b/CITATIONS.md index b2570fc..704e81f 100644 --- a/CITATIONS.md +++ b/CITATIONS.md @@ -10,10 +10,13 @@ ## Pipeline tools -- [FastQC](https://www.bioinformatics.babraham.ac.uk/projects/fastqc/) +- [bedgovcf](https://github.com/nvnieuwk/bedgovcf) +- [ExomeDepth](https://pubmed.ncbi.nlm.nih.gov/22942019/) - > Andrews, S. (2010). FastQC: A Quality Control Tool for High Throughput Sequence Data [Online]. + > Plagnol V, Curtis J, Epstein M, Mok KY, Stebbings E, Grigoriadou S, Wood NW, Hambleton S, Burns SO, Thrasher AJ, Kumararatne D, Doffinger R, Nejentsev S. A robust model for read count data in exome sequencing experiments and implications for copy number variant calling. Bioinformatics. 2012 Nov 1;28(21):2747-54. doi: 10.1093/bioinformatics/bts526. Epub 2012 Aug 31. PMID: 22942019; PMCID: PMC3476336. +- [EnsemblVEP](https://pubmed.ncbi.nlm.nih.gov/27268795/) + > McLaren W, Gil L, Hunt SE, et al.: The Ensembl Variant Effect Predictor. Genome Biol. 2016 Jun 6;17(1):122. doi: 10.1186/s13059-016-0974-4. PubMed PMID: 27268795; PubMed Central PMCID: PMC4893825. - [MultiQC](https://pubmed.ncbi.nlm.nih.gov/27312411/) > Ewels P, Magnusson M, Lundin S, Käller M. MultiQC: summarize analysis results for multiple tools and samples in a single report. Bioinformatics. 2016 Oct 1;32(19):3047-8. doi: 10.1093/bioinformatics/btw354. Epub 2016 Jun 16. 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zm=Uq0#T*%OOBY{1OW6bHe|`7g2_UE}i5KdLpWD;SG!wrthx*N`YJ(>z|5q9Rk7tW> p$^W)m|Nk@4;Q#PH^rE?)&%=32f79kWifM5(jpiCgO + [![GitHub Actions CI Status](https://github.com/nf-cmgg/exomecnv/actions/workflows/ci.yml/badge.svg)](https://github.com/nf-cmgg/exomecnv/actions/workflows/ci.yml) [![GitHub Actions Linting Status](https://github.com/nf-cmgg/exomecnv/actions/workflows/linting.yml/badge.svg)](https://github.com/nf-cmgg/exomecnv/actions/workflows/linting.yml)[![Cite with Zenodo](http://img.shields.io/badge/DOI-10.5281/zenodo.XXXXXXX-1073c8?labelColor=000000)](https://doi.org/10.5281/zenodo.XXXXXXX) [![nf-test](https://img.shields.io/badge/unit_tests-nf--test-337ab7.svg)](https://www.nf-test.com) @@ -11,59 +12,57 @@ ## Introduction -**nf-cmgg/exomecnv** is a bioinformatics pipeline that ... - - - - - +**nf-cmgg/exomecnv** is a bioinformatics pipeline that can be used to call copy number variations (CNVs) from exome sequencing data with ExomeDepth and annotate these with EnsemblVEP. It takes a samplesheet with cram or bam files and their index files as input, generates read count data, calls CNVs and ends with an annotation. It is also possible to take a samplesheet with VCF files and their index files as input and only execute the annotation. ## Pipeline Summary -1. CRAM -> BAM -2. ExomeDepth counting per sample -3. Create count file for all samples per pool -4. ExomeDepth CNV calling per sample -5. Convert BED to VCF -6. Annotation per sample (VEP + custom annotation) => make it possible to run from here (from VCF file) -7. Output as XLSX file +1. Convert CRAM to BAM if CRAM files are provided (optional) +2. ExomeDepth counting per sample (autosomal and chrX separated) +3. Merge count files per pool (autosomal and chrX stay separated) +4. ExomeDepth CNV calling per sample (autosomal and chrX stay separated) +5. Merge CNV calling files per sample (autosomal and chrX are merged) +6. Convert merged files to VCF +7. Generate index files for these VCF files +6. Annotate VCF files with EnsemblVEP + + ## Usage > [!NOTE] > If you are new to Nextflow and nf-core, please refer to [this page](https://nf-co.re/docs/usage/installation) on how to set-up Nextflow. Make sure to [test your setup](https://nf-co.re/docs/usage/introduction#how-to-run-a-pipeline) with `-profile test` before running the workflow on actual data. - +Each row represents a sample with the associated pool and family, followed by the optional paths to the CRAM/CRAI or VCF/TBI files, depending on which tasks should be executed. Now, you can run the pipeline using: - - ```bash nextflow run nf-cmgg/exomecnv \ - -profile \ - --input samplesheet.csv \ - --outdir + -profile \ + --input /path/to/samplesheet.csv \ + --outdir /path/to/outdir \ + --vep_cache /path/to/vep_cache \ + --exomedepth \ + --annotate +``` +to execute the ExomeDepth workflow, followed by an EnsemblVEP annotation on CRAM/CRAI (or BAM/BAI) files provided in the samplesheet. The --annotate parameter is optional. If not provided, only the ExomeDepth workflow will be executed. +It is also possible to run the pipeline using: +```bash +nextflow run nf-cmgg/exomecnv \ + -profile \ + --input /path/to/samplesheet.csv \ + --outdir /path/to/outdir \ + --vep_cache /path/to/vep_cache ``` +to skip the ExomeDepth workflow and only execute the EnsemblVEP annotation on VCF/TBI files provided in the samplesheet. > [!WARNING] > Please provide pipeline parameters via the CLI or Nextflow `-params-file` option. Custom config files including those provided by the `-c` Nextflow option can be used to provide any configuration _**except for parameters**_; diff --git a/conf/modules.config b/conf/modules.config index 9a622fd..4e76fce 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -29,6 +29,11 @@ process { withName: 'BEDGOVCF' { ext.args = { "--skip 1" } + ext.prefix = {"${meta.id}_CNVs_ExomeDepth"} + publishDir = [ + path: {"$params.outdir/exomedepth/cnv_call"}, + mode: params.publish_dir_mode + ] } withName: 'ENSEMBLVEP_VEP' { @@ -46,4 +51,11 @@ process { '--transcript_filter "stable_id match ENST"' ].join(' ').trim()} } + + withName: 'TABIX_TABIX' { + publishDir = [ + path: {"$params.outdir/exomedepth/cnv_call"}, + mode: params.publish_dir_mode + ] + } } diff --git a/docs/images/nfcore-exomecnv_logo.png b/docs/images/nfcore-exomecnv_logo.png index 1458c8f0d15ee234e62af9075b3aa51cd309819d..8427f0efa7ecaa8c7f2a9abc6609221721b76c4f 100644 GIT binary patch literal 89499 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@@ "vep_cache": { "type": "string", "format": "directory-path", - "description": "Path to the VEP cache" + "description": "Path to the VEP cache", + "exists": true }, "annotate": { "type": "boolean", diff --git a/subworkflows/local/exomedepth/main.nf b/subworkflows/local/exomedepth/main.nf index 4e24e86..cfebd74 100644 --- a/subworkflows/local/exomedepth/main.nf +++ b/subworkflows/local/exomedepth/main.nf @@ -31,9 +31,7 @@ workflow EXOMEDEPTH { // Importing and convert the input files passed through the parameters to channels // Branch into CRAM and BAM files - ch_samplesheet.map { meta, cram, crai, vcf, tbi -> - [meta, cram, crai]} - .branch { meta, cram, crai -> + ch_samplesheet.branch { meta, cram, crai -> CRAM: cram.extension == "cram" BAM: cram.extension == "bam" } diff --git a/subworkflows/local/vcf_annotation/main.nf b/subworkflows/local/vcf_annotation/main.nf index 45fe813..76dc2cc 100644 --- a/subworkflows/local/vcf_annotation/main.nf +++ b/subworkflows/local/vcf_annotation/main.nf @@ -12,7 +12,7 @@ include { ENSEMBLVEP_VEP as VEP } from '../../../modules/nf-co ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ */ -workflow ENSEMBLVEP { +workflow VCF_ANNOTATION { take: diff --git a/workflows/exomecnv.nf b/workflows/exomecnv.nf index 80057dd..a4d7e22 100644 --- a/workflows/exomecnv.nf +++ b/workflows/exomecnv.nf @@ -11,9 +11,10 @@ include { softwareVersionsToYAML } from '../subworkflows/nf-core/utils_nfcore_pi include { methodsDescriptionText } from '../subworkflows/local/utils_nfcore_exomecnv_pipeline' // local -include { EXOMEDEPTH } from '../subworkflows/local/exomedepth/main' -include { ENSEMBLVEP } from '../subworkflows/local/vcf_annotation/main' -include { TABIX_TABIX as TABIX } from '../modules/nf-core/tabix/tabix/main' +include { EXOMEDEPTH } from '../subworkflows/local/exomedepth/main' +include { VCF_ANNOTATION as ANNOTATION_FROM_CRAM } from '../subworkflows/local/vcf_annotation/main' +include { VCF_ANNOTATION as ANNOTATION_FROM_VCF } from '../subworkflows/local/vcf_annotation/main' +include { TABIX_TABIX as TABIX } from '../modules/nf-core/tabix/tabix/main' /* ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ @@ -39,12 +40,17 @@ workflow EXOMECNV { ch_vep_cache = Channel.fromPath(params.vep_cache).collect() - if (!params.annotate){ + ch_samplesheet.branch { meta, cram, crai, vcf, tbi -> + vcf: vcf + return [ meta, cram, crai, vcf, tbi] + no_vcf: !vcf + return [ meta, cram, crai]} + .set{ ch_input } // ExomeDepth - + if (ch_input.no_vcf) { if (params.exomedepth) { - EXOMEDEPTH (ch_samplesheet) + EXOMEDEPTH (ch_input.no_vcf) ch_versions = EXOMEDEPTH.out.versions // Index files for VCF @@ -55,22 +61,25 @@ workflow EXOMECNV { // EnsemblVEP after ExomeDepth - ENSEMBLVEP ( ch_exomedepth_vcf, ch_fasta, ch_vep_cache ) - ch_versions = ch_versions.mix(ENSEMBLVEP.out.versions) + if (params.annotate) { + + ANNOTATION_FROM_CRAM ( ch_exomedepth_vcf, ch_fasta, ch_vep_cache ) + ch_versions = ch_versions.mix(ANNOTATION_FROM_CRAM.out.versions) + } } } // EnsemblVEP on VCF input file - else { - ch_vcf = ch_samplesheet + if (ch_input.vcf) { + ch_vcf = ch_input.vcf .map { meta,bam,bai,vcf,tbi -> [[id:meta.id], vcf, tbi]} - ENSEMBLVEP ( + ANNOTATION_FROM_VCF ( ch_vcf, ch_fasta, ch_vep_cache ) - ch_versions = ENSEMBLVEP.out.versions + ch_versions = ANNOTATION_FROM_VCF.out.versions } // Collate and save software versions From 3d18226d026f8c63e3c7004a092920ecc79f80d4 Mon Sep 17 00:00:00 2001 From: BertGalle Date: Mon, 10 Jun 2024 16:31:30 +0200 Subject: [PATCH 55/64] output.md and usage.md + suggestions --- CITATIONS.md | 6 +- README.md | 25 ++-- assets/samplesheet.csv | 2 +- ...eDepth_call.R => ExomeDepth_cnv_calling.R} | 0 docs/images/mqc_fastqc_adapter.png | Bin 23458 -> 0 bytes docs/images/mqc_fastqc_counts.png | Bin 33918 -> 0 bytes docs/images/mqc_fastqc_quality.png | Bin 55769 -> 0 bytes docs/output.md | 118 ++++++++++++++---- docs/usage.md | 78 ++++++------ modules/local/exomedepth/cnv_call/main.nf | 2 +- 10 files changed, 152 insertions(+), 79 deletions(-) rename bin/{CNV_ExomeDepth_call.R => ExomeDepth_cnv_calling.R} (100%) delete mode 100755 docs/images/mqc_fastqc_adapter.png delete mode 100755 docs/images/mqc_fastqc_counts.png delete mode 100755 docs/images/mqc_fastqc_quality.png diff --git a/CITATIONS.md b/CITATIONS.md index 704e81f..e0ab4bc 100644 --- a/CITATIONS.md +++ b/CITATIONS.md @@ -9,11 +9,15 @@ > Di Tommaso P, Chatzou M, Floden EW, Barja PP, Palumbo E, Notredame C. Nextflow enables reproducible computational workflows. Nat Biotechnol. 2017 Apr 11;35(4):316-319. doi: 10.1038/nbt.3820. PubMed PMID: 28398311. ## Pipeline tools +- [SAMtools](https://pubmed.ncbi.nlm.nih.gov/19505943/) + > Li H, Handsaker B, Wysoker A, Fennell T, Ruan J, Homer N, Marth G, Abecasis G, Durbin R; 1000 Genome Project Data Processing Subgroup. The Sequence Alignment/Map format and SAMtools. Bioinformatics. 2009 Aug 15;25(16):2078-9. doi: 10.1093/bioinformatics/btp352. Epub 2009 Jun 8. PMID: 19505943; PMCID: PMC2723002. + +- [BedGoVcf](https://github.com/nvnieuwk/bedgovcf) -- [bedgovcf](https://github.com/nvnieuwk/bedgovcf) - [ExomeDepth](https://pubmed.ncbi.nlm.nih.gov/22942019/) > Plagnol V, Curtis J, Epstein M, Mok KY, Stebbings E, Grigoriadou S, Wood NW, Hambleton S, Burns SO, Thrasher AJ, Kumararatne D, Doffinger R, Nejentsev S. A robust model for read count data in exome sequencing experiments and implications for copy number variant calling. Bioinformatics. 2012 Nov 1;28(21):2747-54. doi: 10.1093/bioinformatics/bts526. Epub 2012 Aug 31. PMID: 22942019; PMCID: PMC3476336. + - [EnsemblVEP](https://pubmed.ncbi.nlm.nih.gov/27268795/) > McLaren W, Gil L, Hunt SE, et al.: The Ensembl Variant Effect Predictor. Genome Biol. 2016 Jun 6;17(1):122. doi: 10.1186/s13059-016-0974-4. PubMed PMID: 27268795; PubMed Central PMCID: PMC4893825. diff --git a/README.md b/README.md index 292d09e..af72f85 100644 --- a/README.md +++ b/README.md @@ -12,18 +12,19 @@ ## Introduction -**nf-cmgg/exomecnv** is a bioinformatics pipeline that can be used to call copy number variations (CNVs) from exome sequencing data with ExomeDepth and annotate these with EnsemblVEP. It takes a samplesheet with cram or bam files and their index files as input, generates read count data, calls CNVs and ends with an annotation. It is also possible to take a samplesheet with VCF files and their index files as input and only execute the annotation. +**nf-cmgg/exomecnv** is a bioinformatics pipeline that can be used to call copy number variations (CNVs) from exome sequencing data with ExomeDepth and annotate these with EnsemblVEP. It takes a samplesheet with CRAM or BAM files and their index files as input, generates read count data, calls CNVs and ends with an annotation. It is also possible to take a samplesheet with VCF files and their index files as input and only execute the annotation. ## Pipeline Summary -1. Convert CRAM to BAM if CRAM files are provided (optional) -2. ExomeDepth counting per sample (autosomal and chrX separated) -3. Merge count files per pool (autosomal and chrX stay separated) -4. ExomeDepth CNV calling per sample (autosomal and chrX stay separated) -5. Merge CNV calling files per sample (autosomal and chrX are merged) -6. Convert merged files to VCF -7. Generate index files for these VCF files -6. Annotate VCF files with EnsemblVEP +1. Input samplesheet check +2. Convert CRAM to BAM if CRAM files are provided (optional) +3. ExomeDepth counting per sample (autosomal and chrX are separated) +4. Merge count files per pool (autosomal and chrX remain separated) +5. ExomeDepth CNV calling per sample (autosomal and chrX remain separated) +6. Merge CNV calling files per sample (autosomal and chrX are merged) +7. Convert merged files to VCF +8. Generate index files for these VCF files +9. Annotate VCF files with EnsemblVEP @@ -40,7 +41,7 @@ sample,pool,family,cram,crai,vcf,tbi sample1,poolM,Fam1,/path/to/sample1.cram,/path/to/sample1.crai sample2,poolF,Fam2,/path/to/sample2.cram,/path/to/sample2.crai,/path/to/sample2.vcf,/path/to/sample2.vcf.tbi ``` -Each row represents a sample with the associated pool and family, followed by the optional paths to the CRAM/CRAI or VCF/TBI files, depending on which tasks should be executed. +Each row represents a sample with the associated pool and family, followed by the optional paths to the CRAM/CRAI and/or VCF/TBI files, depending on which tasks should be executed. Now, you can run the pipeline using: @@ -70,9 +71,9 @@ to skip the ExomeDepth workflow and only execute the EnsemblVEP annotation on VC ## Credits -nf-cmgg/exomecnv was originally written by nvnieuwk. +nf-cmgg/exomecnv was originally written by [BertGalle](.https://github.com/BertGalle) and [Toros](.https://github.com/ToonRosseel). -We thank the following people for their extensive assistance in the development of this pipeline: +We thank the following people for their extensive assistance in the development of this pipeline: [nvnieuwk](.https://github.com/nvnieuwk) diff --git a/assets/samplesheet.csv b/assets/samplesheet.csv index d25fe22..cbf3cf7 100644 --- a/assets/samplesheet.csv +++ b/assets/samplesheet.csv @@ -1,4 +1,4 @@ -sample,pool,family,cram,crai +sample,pool,family,cram,crai,vcf,tbi Sample1,poolM,Fam1,https://raw.githubusercontent.com/nf-cmgg/test-datasets/exomecnv/data/genomics/homo_sapiens/illumina/cram/Sample1.cram,https://raw.githubusercontent.com/nf-cmgg/test-datasets/exomecnv/data/genomics/homo_sapiens/illumina/cram/Sample1.cram.crai Sample2,poolM,Fam2,https://raw.githubusercontent.com/nf-cmgg/test-datasets/exomecnv/data/genomics/homo_sapiens/illumina/cram/Sample2.cram,https://raw.githubusercontent.com/nf-cmgg/test-datasets/exomecnv/data/genomics/homo_sapiens/illumina/cram/Sample2.cram.crai Sample3,poolM,Fam3,https://raw.githubusercontent.com/nf-cmgg/test-datasets/exomecnv/data/genomics/homo_sapiens/illumina/cram/Sample3.cram,https://raw.githubusercontent.com/nf-cmgg/test-datasets/exomecnv/data/genomics/homo_sapiens/illumina/cram/Sample3.cram.crai diff --git a/bin/CNV_ExomeDepth_call.R b/bin/ExomeDepth_cnv_calling.R similarity index 100% rename from bin/CNV_ExomeDepth_call.R rename to bin/ExomeDepth_cnv_calling.R diff --git 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Most of the plots are taken from the MultiQC report, which summarises results at the end of the pipeline. +This document describes the output produced by the pipeline. The directories listed below will be created in the results directory after the pipeline has finished. All paths are relative to the top-level results directory. @@ -12,48 +12,120 @@ The directories listed below will be created in the results directory after the The pipeline is built using [Nextflow](https://www.nextflow.io/) and processes data using the following steps: -- [FastQC](#fastqc) - Raw read QC -- [MultiQC](#multiqc) - Aggregate report describing results and QC from the whole pipeline +- [Cram Prepare](#cram-prepare) +- [ExomeDepth count](#exomedepth-count) +- [Count merge](#count-merge) +- [ExomeDepth CNV call](#exomedepth-cnv-call) +- [CNV merge](#cnv-merge) +- [BedGoVcf](#bedgovcf) +- [Tabix](#tabix) +- [EnsemblVEP](#ensemblvep) - [Pipeline information](#pipeline-information) - Report metrics generated during the workflow execution -### FastQC +### Cram Prepare + +If CRAM/CRAI files were provided in the samplesheet, they will be converted into BAM/BAI first before continuing with the pipeline. + +

    +Output files + +- `cram/` + - `.bam`: BAM file +- `cram/` + - `.bam.bai`: BAI index file +
    + +### ExomeDepth count + +ExomeDepth will generate 2 count files for each sample: 1 for autosomal chromosomes and 1 for chrX. + +
    +Output files + +- `exomedepth/` + - `counts/` + - `_autosomal.txt`: Count file for autosomal chromosomes per sample + - `_chrX.txt`: Count file for chrX per sample + +
    + +### Count merge + +All samples sharing the same pool will be merged together since CNV calling in the ExomeDepth workflow is executed per pool.
    Output files -- `fastqc/` - - `*_fastqc.html`: FastQC report containing quality metrics. - - `*_fastqc.zip`: Zip archive containing the FastQC report, tab-delimited data file and plot images. +- `exomedepth/` + - `counts/` + - `_autosomal.txt`: Count file for autosomal chromosomes per pool + - `_chrX.txt`: Count file for chrX per pool
    -[FastQC](http://www.bioinformatics.babraham.ac.uk/projects/fastqc/) gives general quality metrics about your sequenced reads. It provides information about the quality score distribution across your reads, per base sequence content (%A/T/G/C), adapter contamination and overrepresented sequences. For further reading and documentation see the [FastQC help pages](http://www.bioinformatics.babraham.ac.uk/projects/fastqc/Help/). +### ExomeDepth CNV call -![MultiQC - FastQC sequence counts plot](images/mqc_fastqc_counts.png) +ExomeDepth will generate 2 CNV files for each sample: 1 for autosomal chromosomes and 1 for chrX. -![MultiQC - FastQC mean quality scores plot](images/mqc_fastqc_quality.png) +
    +Output files + +- `exomedepth/` + - `cnv_call/` + - `_CNVs_ExomeDepth_autosomal.txt`: CNV file for autosomal chromosomes + - `_CNVs_ExomeDepth_chrX.txt`: CNV file for chrX -![MultiQC - FastQC adapter content plot](images/mqc_fastqc_adapter.png) -:::note -The FastQC plots displayed in the MultiQC report shows _untrimmed_ reads. They may contain adapter sequence and potentially regions with low quality. -::: +
    -### MultiQC +### CNV merge + +The 2 CNV files will be merged into 1 complete file.
    Output files -- `multiqc/` - - `multiqc_report.html`: a standalone HTML file that can be viewed in your web browser. - - `multiqc_data/`: directory containing parsed statistics from the different tools used in the pipeline. - - `multiqc_plots/`: directory containing static images from the report in various formats. +- `exomedepth/` + - `cnv_call/` + - `_CNVs_ExomeDepth.txt`: Merged CNV file
    -[MultiQC](http://multiqc.info) is a visualization tool that generates a single HTML report summarising all samples in your project. Most of the pipeline QC results are visualised in the report and further statistics are available in the report data directory. +### BedGoVcf -Results generated by MultiQC collate pipeline QC from supported tools e.g. FastQC. The pipeline has special steps which also allow the software versions to be reported in the MultiQC output for future traceability. For more information about how to use MultiQC reports, see . +The merged CNV file is converted into a VCF file. + +
    +Output files + +- `exomedepth/` + - `cnv_call/` + - `_CNVs_ExomeDepth.vcf.gz`: VCF file + +
    + +### Tabix + +A TBI index file is generated for each VCF file. While these TBI index files are optional for EnsemblVEP, we still generate them as a best practice to ensure better performance and reliability. + +
    +Output files + +- `exomedepth/` + - `cnv_call/` + - `_CNVs_ExomeDepth.vcf.gz.tbi`: TBI index file + +
    + +### EnsemblVEP + +
    +Output files + +- `vep/` + - `.vep.vcf.gz`: VEP annotated file + +
    ### Pipeline information @@ -63,9 +135,9 @@ Results generated by MultiQC collate pipeline QC from supported tools e.g. FastQ - `pipeline_info/` - Reports generated by Nextflow: `execution_report.html`, `execution_timeline.html`, `execution_trace.txt` and `pipeline_dag.dot`/`pipeline_dag.svg`. - Reports generated by the pipeline: `pipeline_report.html`, `pipeline_report.txt` and `software_versions.yml`. The `pipeline_report*` files will only be present if the `--email` / `--email_on_fail` parameter's are used when running the pipeline. - - Reformatted samplesheet files used as input to the pipeline: `samplesheet.valid.csv`. - - Parameters used by the pipeline run: `params.json`. + +\ [Nextflow](https://www.nextflow.io/docs/latest/tracing.html) provides excellent functionality for generating various reports relevant to the running and execution of the pipeline. This will allow you to troubleshoot errors with the running of the pipeline, and also provide you with other information such as launch commands, run times and resource usage. diff --git a/docs/usage.md b/docs/usage.md index 3be8450..f8f5c85 100644 --- a/docs/usage.md +++ b/docs/usage.md @@ -2,51 +2,41 @@ > _Documentation of pipeline parameters is generated automatically from the pipeline schema and can no longer be found in markdown files._ -## Introduction - - - ## Samplesheet input -You will need to create a samplesheet with information about the samples you would like to analyse before running the pipeline. Use this parameter to specify its location. It has to be a comma-separated file with 3 columns, and a header row as shown in the examples below. +You will need to create a samplesheet with information about the samples you would like to analyse before running the pipeline. Use this parameter to specify its location. It has to be a comma-separated file with up to 7 columns, and a header row as shown in the examples below. ```bash --input '[path to samplesheet file]' ``` -### Multiple runs of the same sample - -The `sample` identifiers have to be the same when you have re-sequenced the same sample more than once e.g. to increase sequencing depth. The pipeline will concatenate the raw reads before performing any downstream analysis. Below is an example for the same sample sequenced across 3 lanes: - -```csv title="samplesheet.csv" -sample,fastq_1,fastq_2 -CONTROL_REP1,AEG588A1_S1_L002_R1_001.fastq.gz,AEG588A1_S1_L002_R2_001.fastq.gz -CONTROL_REP1,AEG588A1_S1_L003_R1_001.fastq.gz,AEG588A1_S1_L003_R2_001.fastq.gz -CONTROL_REP1,AEG588A1_S1_L004_R1_001.fastq.gz,AEG588A1_S1_L004_R2_001.fastq.gz -``` - ### Full samplesheet -The pipeline will auto-detect whether a sample is single- or paired-end using the information provided in the samplesheet. The samplesheet can have as many columns as you desire, however, there is a strict requirement for the first 3 columns to match those defined in the table below. +The samplesheet has a strict requirement for the first 7 columns to match those defined in the table below. The pipeline will auto-detect whether a sample contains VCF files using the information provided in the samplesheet. In that case, it will skip the ExomeDepth workflow for these samples and only execute EnsemblVEP. -A final samplesheet file consisting of both single- and paired-end data may look something like the one below. This is for 6 samples, where `TREATMENT_REP3` has been sequenced twice. +A final samplesheet file consisting of both samples to run the full workflow and samples to only re-annotate with EnsemblVEP may look something like the one below. ```csv title="samplesheet.csv" -sample,fastq_1,fastq_2 -CONTROL_REP1,AEG588A1_S1_L002_R1_001.fastq.gz,AEG588A1_S1_L002_R2_001.fastq.gz -CONTROL_REP2,AEG588A2_S2_L002_R1_001.fastq.gz,AEG588A2_S2_L002_R2_001.fastq.gz -CONTROL_REP3,AEG588A3_S3_L002_R1_001.fastq.gz,AEG588A3_S3_L002_R2_001.fastq.gz -TREATMENT_REP1,AEG588A4_S4_L003_R1_001.fastq.gz, -TREATMENT_REP2,AEG588A5_S5_L003_R1_001.fastq.gz, -TREATMENT_REP3,AEG588A6_S6_L003_R1_001.fastq.gz, -TREATMENT_REP3,AEG588A6_S6_L004_R1_001.fastq.gz, +sample,pool,family,cram,crai,vcf,tbi +Sample1,poolM,Fam1,/path/to/cram/Sample1,/path/to/crai/Sample1 +Sample2,poolM,Fam2,/path/to/cram/Sample2,/path/to/crai/Sample2 +Sample3,poolM,Fam2,/path/to/cram/Sample3,/path/to/crai/Sample3 +Sample4,poolF,Fam3,/path/to/cram/Sample4,/path/to/crai/Sample4,/path/to/vcf/Sample4,/path/to/tbi/Sample4 +Sample5,poolF,Fam4,/path/to/cram/Sample5,/path/to/crai/Sample5,/path/to/vcf/Sample5,/path/to/tbi/Sample5 +Sample6,poolF,Fam5,/path/to/cram/Sample6,/path/to/crai/Sample6,/path/to/vcf/Sample6,/path/to/tbi/Sample6 + ``` +### All samplesheet options | Column | Description | | --------- | -------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- | -| `sample` | Custom sample name. This entry will be identical for multiple sequencing libraries/runs from the same sample. Spaces in sample names are automatically converted to underscores (`_`). | -| `fastq_1` | Full path to FastQ file for Illumina short reads 1. File has to be gzipped and have the extension ".fastq.gz" or ".fq.gz". | -| `fastq_2` | Full path to FastQ file for Illumina short reads 2. File has to be gzipped and have the extension ".fastq.gz" or ".fq.gz". | +| `sample` | Custom sample name. Cannot contain spaces and has to be unique. | +| `pool` | Pool name for the current sample. Samples sharing the same pool will be merged together during the pipeline since CNV calling in the ExomeDepth workflow is executed per pool. Cannot contain spaces. | +| `family` | Family name for the current sample. Samples sharing this family name will be excluded from the reference set to ensure that CNVs common to this family are not excluded. Cannot contain spaces. +| `cram` | Path to the CRAM (or BAM) file to be used by the pipeline for the current sample.| +| `crai` | Path to the CRAM (or BAI) index file.| +| `vcf` | Path to the VCF file to be used by the pipeline for the current sample. When this is provided, the pipeline will skip to the annotation. | +| `tbi` | Path to the TBI index file. | | An [example samplesheet](../assets/samplesheet.csv) has been provided with the pipeline. @@ -55,7 +45,13 @@ An [example samplesheet](../assets/samplesheet.csv) has been provided with the p The typical command for running the pipeline is as follows: ```bash -nextflow run nf-cmgg/exomecnv --input ./samplesheet.csv --outdir ./results --genome GRCh37 -profile docker +nextflow run nf-cmgg/exomecnv \ + -profile docker \ + --input /path/to/samplesheet.csv \ + --outdir /path/to/outdir \ + --vep_cache /path/to/vep_cache \ + --exomedepth \ + --annotate ``` This will launch the pipeline with the `docker` configuration profile. See below for more information about profiles. @@ -73,9 +69,9 @@ If you wish to repeatedly use the same parameters for multiple runs, rather than Pipeline settings can be provided in a `yaml` or `json` file via `-params-file `. -:::warning +> **_WARNING:_** Do not use `-c ` to specify parameters as this will result in errors. Custom config files specified with `-c` must only be used for [tuning process resource specifications](https://nf-co.re/docs/usage/configuration#tuning-workflow-resources), other infrastructural tweaks (such as output directories), or module arguments (args). -::: + The above pipeline run specified with a params file in yaml format: @@ -86,9 +82,11 @@ nextflow run nf-cmgg/exomecnv -profile docker -params-file params.yaml with `params.yaml` containing: ```yaml -input: './samplesheet.csv' -outdir: './results/' -genome: 'GRCh37' +input: '/path/to/samplesheet.csv' +outdir: '/path/to/outdir' +vep_cache: '/path/to/vep_cache' +exomedepth: true +annotate: true <...> ``` @@ -112,15 +110,14 @@ This version number will be logged in reports when you run the pipeline, so that To further assist in reproducbility, you can use share and re-use [parameter files](#running-the-pipeline) to repeat pipeline runs with the same settings without having to write out a command with every single parameter. -:::tip +> **_NOTE:_** If you wish to share such profile (such as upload as supplementary material for academic publications), make sure to NOT include cluster specific paths to files, nor institutional specific profiles. -::: ## Core Nextflow arguments -:::note +> **_NOTE:_** These options are part of Nextflow and use a _single_ hyphen (pipeline parameters use a double-hyphen). -::: + ### `-profile` @@ -128,9 +125,8 @@ Use this parameter to choose a configuration profile. Profiles can give configur Several generic profiles are bundled with the pipeline which instruct the pipeline to use software packaged using different methods (Docker, Singularity, Podman, Shifter, Charliecloud, Apptainer, Conda) - see below. -:::info +> **_NOTE:_** We highly recommend the use of Docker or Singularity containers for full pipeline reproducibility, however when this is not possible, Conda is also supported. -::: The pipeline also dynamically loads configurations from [https://github.com/nf-core/configs](https://github.com/nf-core/configs) when it runs, making multiple config profiles for various institutional clusters available at run time. For more information and to see if your system is available in these configs please see the [nf-core/configs documentation](https://github.com/nf-core/configs#documentation). diff --git a/modules/local/exomedepth/cnv_call/main.nf b/modules/local/exomedepth/cnv_call/main.nf index b6ce189..b87b5ea 100644 --- a/modules/local/exomedepth/cnv_call/main.nf +++ b/modules/local/exomedepth/cnv_call/main.nf @@ -22,7 +22,7 @@ process CNV_CALL { def VERSION = '1.1.16' """ - CNV_ExomeDepth_call.R \\ + ExomeDepth_cnv_calling.R \\ $sample \\ $countfile \\ $exon_target \\ From 20ddedb0c469204ca88fbec2ac6167f938700b60 Mon Sep 17 00:00:00 2001 From: BertGalle <129084478+BertGalle@users.noreply.github.com> Date: Mon, 10 Jun 2024 16:35:22 +0200 Subject: [PATCH 56/64] Update README.md --- README.md | 4 ++-- 1 file changed, 2 insertions(+), 2 deletions(-) diff --git a/README.md b/README.md index af72f85..f326171 100644 --- a/README.md +++ b/README.md @@ -71,9 +71,9 @@ to skip the ExomeDepth workflow and only execute the EnsemblVEP annotation on VC ## Credits -nf-cmgg/exomecnv was originally written by [BertGalle](.https://github.com/BertGalle) and [Toros](.https://github.com/ToonRosseel). +nf-cmgg/exomecnv was originally written by [BertGalle](https://github.com/BertGalle) and [Toros](https://github.com/ToonRosseel). -We thank the following people for their extensive assistance in the development of this pipeline: [nvnieuwk](.https://github.com/nvnieuwk) +We thank the following people for their extensive assistance in the development of this pipeline: [nvnieuwk](https://github.com/nvnieuwk) From 160d8e070809a1c0ac1c705263c6adf50fe2b42a Mon Sep 17 00:00:00 2001 From: BertGalle <129084478+BertGalle@users.noreply.github.com> Date: Mon, 10 Jun 2024 16:39:19 +0200 Subject: [PATCH 57/64] Small edit readme --- docs/output.md | 1 - 1 file changed, 1 deletion(-) diff --git a/docs/output.md b/docs/output.md index ae9b9d3..1a615d8 100644 --- a/docs/output.md +++ b/docs/output.md @@ -31,7 +31,6 @@ If CRAM/CRAI files were provided in the samplesheet, they will be converted into - `cram/` - `.bam`: BAM file -- `cram/` - `.bam.bai`: BAI index file From c073d69701a61b7ecffb137ef885a87f1fb8cb5e Mon Sep 17 00:00:00 2001 From: BertGalle Date: Tue, 11 Jun 2024 14:34:52 +0200 Subject: [PATCH 58/64] usage.md edit + version fixes --- docs/usage.md | 4 ++-- workflows/exomecnv.nf | 6 ++++-- 2 files changed, 6 insertions(+), 4 deletions(-) diff --git a/docs/usage.md b/docs/usage.md index f8f5c85..abbe15e 100644 --- a/docs/usage.md +++ b/docs/usage.md @@ -31,8 +31,8 @@ Sample6,poolF,Fam5,/path/to/cram/Sample6,/path/to/crai/Sample6,/path/to/vcf/Samp | Column | Description | | --------- | -------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- | | `sample` | Custom sample name. Cannot contain spaces and has to be unique. | -| `pool` | Pool name for the current sample. Samples sharing the same pool will be merged together during the pipeline since CNV calling in the ExomeDepth workflow is executed per pool. Cannot contain spaces. | -| `family` | Family name for the current sample. Samples sharing this family name will be excluded from the reference set to ensure that CNVs common to this family are not excluded. Cannot contain spaces. +| `pool` | Pool name for the current sample. Samples sharing the same pool will be analysed together since CNV calling in the ExomeDepth workflow is executed per pool. It's recommended to put the (genetically speaking) female and male samples in seperate pools. The samples in the same pool should be enriched with the same library prep kit. An optimized aggregated reference set is chosen from the samples of the pool. Cannot contain spaces. | +| `family` | Family name for the current sample. Samples sharing this family name will be excluded from the aggregate reference set to ensure that common CNVs to this family will be detected. ExomeDepth assumes that the CNV of interest is absent from the aggregate reference set. Cannot contain spaces. | `cram` | Path to the CRAM (or BAM) file to be used by the pipeline for the current sample.| | `crai` | Path to the CRAM (or BAI) index file.| | `vcf` | Path to the VCF file to be used by the pipeline for the current sample. When this is provided, the pipeline will skip to the annotation. | diff --git a/workflows/exomecnv.nf b/workflows/exomecnv.nf index a4d7e22..b37d848 100644 --- a/workflows/exomecnv.nf +++ b/workflows/exomecnv.nf @@ -51,11 +51,12 @@ workflow EXOMECNV { if (ch_input.no_vcf) { if (params.exomedepth) { EXOMEDEPTH (ch_input.no_vcf) - ch_versions = EXOMEDEPTH.out.versions + ch_versions = ch_versions.mix(EXOMEDEPTH.out.versions) // Index files for VCF TABIX ( EXOMEDEPTH.out.vcf ) + ch_versions = ch_versions.mix(TABIX.out.versions) ch_exomedepth_vcf = EXOMEDEPTH.out.vcf .join(TABIX.out.tbi) @@ -65,6 +66,7 @@ workflow EXOMECNV { ANNOTATION_FROM_CRAM ( ch_exomedepth_vcf, ch_fasta, ch_vep_cache ) ch_versions = ch_versions.mix(ANNOTATION_FROM_CRAM.out.versions) + } } } @@ -79,7 +81,7 @@ workflow EXOMECNV { ANNOTATION_FROM_VCF ( ch_vcf, ch_fasta, ch_vep_cache ) - ch_versions = ANNOTATION_FROM_VCF.out.versions + ch_versions = ch_versions.mix(ANNOTATION_FROM_VCF.out.versions) } // Collate and save software versions From 3ed1cbe099959d81788e4ec94133da243151d4b8 Mon Sep 17 00:00:00 2001 From: BertGalle Date: Tue, 11 Jun 2024 14:53:04 +0200 Subject: [PATCH 59/64] Pull request fixes --- CITATIONS.md | 3 +++ README.md | 5 +++++ docs/output.md | 4 +--- docs/usage.md | 29 ++++++++++++++--------------- nextflow_schema.json | 3 +-- 5 files changed, 24 insertions(+), 20 deletions(-) diff --git a/CITATIONS.md b/CITATIONS.md index e0ab4bc..4da5927 100644 --- a/CITATIONS.md +++ b/CITATIONS.md @@ -9,7 +9,9 @@ > Di Tommaso P, Chatzou M, Floden EW, Barja PP, Palumbo E, Notredame C. Nextflow enables reproducible computational workflows. Nat Biotechnol. 2017 Apr 11;35(4):316-319. doi: 10.1038/nbt.3820. PubMed PMID: 28398311. ## Pipeline tools + - [SAMtools](https://pubmed.ncbi.nlm.nih.gov/19505943/) + > Li H, Handsaker B, Wysoker A, Fennell T, Ruan J, Homer N, Marth G, Abecasis G, Durbin R; 1000 Genome Project Data Processing Subgroup. The Sequence Alignment/Map format and SAMtools. Bioinformatics. 2009 Aug 15;25(16):2078-9. doi: 10.1093/bioinformatics/btp352. Epub 2009 Jun 8. PMID: 19505943; PMCID: PMC2723002. - [BedGoVcf](https://github.com/nvnieuwk/bedgovcf) @@ -21,6 +23,7 @@ - [EnsemblVEP](https://pubmed.ncbi.nlm.nih.gov/27268795/) > McLaren W, Gil L, Hunt SE, et al.: The Ensembl Variant Effect Predictor. Genome Biol. 2016 Jun 6;17(1):122. doi: 10.1186/s13059-016-0974-4. PubMed PMID: 27268795; PubMed Central PMCID: PMC4893825. + - [MultiQC](https://pubmed.ncbi.nlm.nih.gov/27312411/) > Ewels P, Magnusson M, Lundin S, Käller M. MultiQC: summarize analysis results for multiple tools and samples in a single report. Bioinformatics. 2016 Oct 1;32(19):3047-8. doi: 10.1093/bioinformatics/btw354. Epub 2016 Jun 16. PubMed PMID: 27312411; PubMed Central PMCID: PMC5039924. diff --git a/README.md b/README.md index f326171..5c6d7f7 100644 --- a/README.md +++ b/README.md @@ -36,11 +36,13 @@ First, prepare a samplesheet with your input data that looks as follows: `samplesheet.csv`: + ```csv sample,pool,family,cram,crai,vcf,tbi sample1,poolM,Fam1,/path/to/sample1.cram,/path/to/sample1.crai sample2,poolF,Fam2,/path/to/sample2.cram,/path/to/sample2.crai,/path/to/sample2.vcf,/path/to/sample2.vcf.tbi ``` + Each row represents a sample with the associated pool and family, followed by the optional paths to the CRAM/CRAI and/or VCF/TBI files, depending on which tasks should be executed. Now, you can run the pipeline using: @@ -54,8 +56,10 @@ nextflow run nf-cmgg/exomecnv \ --exomedepth \ --annotate ``` + to execute the ExomeDepth workflow, followed by an EnsemblVEP annotation on CRAM/CRAI (or BAM/BAI) files provided in the samplesheet. The --annotate parameter is optional. If not provided, only the ExomeDepth workflow will be executed. It is also possible to run the pipeline using: + ```bash nextflow run nf-cmgg/exomecnv \ -profile \ @@ -63,6 +67,7 @@ nextflow run nf-cmgg/exomecnv \ --outdir /path/to/outdir \ --vep_cache /path/to/vep_cache ``` + to skip the ExomeDepth workflow and only execute the EnsemblVEP annotation on VCF/TBI files provided in the samplesheet. > [!WARNING] diff --git a/docs/output.md b/docs/output.md index 1a615d8..951db8e 100644 --- a/docs/output.md +++ b/docs/output.md @@ -32,7 +32,7 @@ If CRAM/CRAI files were provided in the samplesheet, they will be converted into - `cram/` - `.bam`: BAM file - `.bam.bai`: BAI index file - + ### ExomeDepth count @@ -74,7 +74,6 @@ ExomeDepth will generate 2 CNV files for each sample: 1 for autosomal chromosome - `_CNVs_ExomeDepth_autosomal.txt`: CNV file for autosomal chromosomes - `_CNVs_ExomeDepth_chrX.txt`: CNV file for chrX - ### CNV merge @@ -135,7 +134,6 @@ A TBI index file is generated for each VCF file. While these TBI index files are - Reports generated by Nextflow: `execution_report.html`, `execution_timeline.html`, `execution_trace.txt` and `pipeline_dag.dot`/`pipeline_dag.svg`. - Reports generated by the pipeline: `pipeline_report.html`, `pipeline_report.txt` and `software_versions.yml`. The `pipeline_report*` files will only be present if the `--email` / `--email_on_fail` parameter's are used when running the pipeline. - \ diff --git a/docs/usage.md b/docs/usage.md index abbe15e..8a01203 100644 --- a/docs/usage.md +++ b/docs/usage.md @@ -26,17 +26,18 @@ Sample5,poolF,Fam4,/path/to/cram/Sample5,/path/to/crai/Sample5,/path/to/vcf/Samp Sample6,poolF,Fam5,/path/to/cram/Sample6,/path/to/crai/Sample6,/path/to/vcf/Sample6,/path/to/tbi/Sample6 ``` + ### All samplesheet options -| Column | Description | -| --------- | -------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- | -| `sample` | Custom sample name. Cannot contain spaces and has to be unique. | -| `pool` | Pool name for the current sample. Samples sharing the same pool will be analysed together since CNV calling in the ExomeDepth workflow is executed per pool. It's recommended to put the (genetically speaking) female and male samples in seperate pools. The samples in the same pool should be enriched with the same library prep kit. An optimized aggregated reference set is chosen from the samples of the pool. Cannot contain spaces. | -| `family` | Family name for the current sample. Samples sharing this family name will be excluded from the aggregate reference set to ensure that common CNVs to this family will be detected. ExomeDepth assumes that the CNV of interest is absent from the aggregate reference set. Cannot contain spaces. -| `cram` | Path to the CRAM (or BAM) file to be used by the pipeline for the current sample.| -| `crai` | Path to the CRAM (or BAI) index file.| -| `vcf` | Path to the VCF file to be used by the pipeline for the current sample. When this is provided, the pipeline will skip to the annotation. | -| `tbi` | Path to the TBI index file. | | +| Column | Description | +| -------- | ----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- | --- | +| `sample` | Custom sample name. Cannot contain spaces and has to be unique. | +| `pool` | Pool name for the current sample. Samples sharing the same pool will be analysed together since CNV calling in the ExomeDepth workflow is executed per pool. It's recommended to put the (genetically speaking) female and male samples in seperate pools. The samples in the same pool should be enriched with the same library prep kit. An optimized aggregated reference set is chosen from the samples of the pool. Cannot contain spaces. | +| `family` | Family name for the current sample. Samples sharing this family name will be excluded from the aggregate reference set to ensure that common CNVs to this family will be detected. ExomeDepth assumes that the CNV of interest is absent from the aggregate reference set. Cannot contain spaces. | +| `cram` | Path to the CRAM (or BAM) file to be used by the pipeline for the current sample. | +| `crai` | Path to the CRAM (or BAI) index file. | +| `vcf` | Path to the VCF file to be used by the pipeline for the current sample. When this is provided, the pipeline will skip to the annotation. | +| `tbi` | Path to the TBI index file. | | An [example samplesheet](../assets/samplesheet.csv) has been provided with the pipeline. @@ -70,8 +71,7 @@ If you wish to repeatedly use the same parameters for multiple runs, rather than Pipeline settings can be provided in a `yaml` or `json` file via `-params-file `. > **_WARNING:_** -Do not use `-c ` to specify parameters as this will result in errors. Custom config files specified with `-c` must only be used for [tuning process resource specifications](https://nf-co.re/docs/usage/configuration#tuning-workflow-resources), other infrastructural tweaks (such as output directories), or module arguments (args). - +> Do not use `-c ` to specify parameters as this will result in errors. Custom config files specified with `-c` must only be used for [tuning process resource specifications](https://nf-co.re/docs/usage/configuration#tuning-workflow-resources), other infrastructural tweaks (such as output directories), or module arguments (args). The above pipeline run specified with a params file in yaml format: @@ -111,13 +111,12 @@ This version number will be logged in reports when you run the pipeline, so that To further assist in reproducbility, you can use share and re-use [parameter files](#running-the-pipeline) to repeat pipeline runs with the same settings without having to write out a command with every single parameter. > **_NOTE:_** -If you wish to share such profile (such as upload as supplementary material for academic publications), make sure to NOT include cluster specific paths to files, nor institutional specific profiles. +> If you wish to share such profile (such as upload as supplementary material for academic publications), make sure to NOT include cluster specific paths to files, nor institutional specific profiles. ## Core Nextflow arguments > **_NOTE:_** -These options are part of Nextflow and use a _single_ hyphen (pipeline parameters use a double-hyphen). - +> These options are part of Nextflow and use a _single_ hyphen (pipeline parameters use a double-hyphen). ### `-profile` @@ -126,7 +125,7 @@ Use this parameter to choose a configuration profile. Profiles can give configur Several generic profiles are bundled with the pipeline which instruct the pipeline to use software packaged using different methods (Docker, Singularity, Podman, Shifter, Charliecloud, Apptainer, Conda) - see below. > **_NOTE:_** -We highly recommend the use of Docker or Singularity containers for full pipeline reproducibility, however when this is not possible, Conda is also supported. +> We highly recommend the use of Docker or Singularity containers for full pipeline reproducibility, however when this is not possible, Conda is also supported. The pipeline also dynamically loads configurations from [https://github.com/nf-core/configs](https://github.com/nf-core/configs) when it runs, making multiple config profiles for various institutional clusters available at run time. For more information and to see if your system is available in these configs please see the [nf-core/configs documentation](https://github.com/nf-core/configs#documentation). diff --git a/nextflow_schema.json b/nextflow_schema.json index e1b07d9..886e77a 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -340,8 +340,7 @@ "vep_cache": { "type": "string", "format": "directory-path", - "description": "Path to the VEP cache", - "exists": true + "description": "Path to the VEP cache" }, "annotate": { "type": "boolean", From 9e9f0ab4b4c6fe546bca8b3ed47d31d71d4bc4e6 Mon Sep 17 00:00:00 2001 From: BertGalle Date: Tue, 11 Jun 2024 15:34:47 +0200 Subject: [PATCH 60/64] Fix files unchanged --- .github/PULL_REQUEST_TEMPLATE.md | 2 +- .github/workflows/linting.yml | 19 +++++++++---------- .github/workflows/linting_comment.yml | 2 +- 3 files changed, 11 insertions(+), 12 deletions(-) diff --git a/.github/PULL_REQUEST_TEMPLATE.md b/.github/PULL_REQUEST_TEMPLATE.md index 2ccb3c0..a1c8add 100644 --- a/.github/PULL_REQUEST_TEMPLATE.md +++ b/.github/PULL_REQUEST_TEMPLATE.md @@ -17,7 +17,7 @@ Learn more about contributing: [CONTRIBUTING.md](https://github.com/nf-cmgg/exom - [ ] If you've fixed a bug or added code that should be tested, add tests! - [ ] If you've added a new tool - have you followed the pipeline conventions in the [contribution docs](https://github.com/nf-cmgg/exomecnv/tree/master/.github/CONTRIBUTING.md) - [ ] Make sure your code lints (`nf-core lint`). -- [ ] Ensure the test suite passes (`nf-test test main.nf.test -profile test,docker`). +- [ ] Ensure the test suite passes (`nextflow run . -profile test,docker --outdir `). - [ ] Check for unexpected warnings in debug mode (`nextflow run . -profile debug,test,docker --outdir `). - [ ] Usage Documentation in `docs/usage.md` is updated. - [ ] Output Documentation in `docs/output.md` is updated. diff --git a/.github/workflows/linting.yml b/.github/workflows/linting.yml index 073e187..1fcafe8 100644 --- a/.github/workflows/linting.yml +++ b/.github/workflows/linting.yml @@ -14,13 +14,12 @@ jobs: pre-commit: runs-on: ubuntu-latest steps: - - uses: actions/checkout@b4ffde65f46336ab88eb53be808477a3936bae11 # v4 + - uses: actions/checkout@0ad4b8fadaa221de15dcec353f45205ec38ea70b # v4 - - name: Set up Python 3.11 - uses: actions/setup-python@0a5c61591373683505ea898e09a3ea4f39ef2b9c # v5 + - name: Set up Python 3.12 + uses: actions/setup-python@82c7e631bb3cdc910f68e0081d67478d79c6982d # v5 with: - python-version: 3.11 - cache: "pip" + python-version: "3.12" - name: Install pre-commit run: pip install pre-commit @@ -32,14 +31,14 @@ jobs: runs-on: ubuntu-latest steps: - name: Check out pipeline code - uses: actions/checkout@b4ffde65f46336ab88eb53be808477a3936bae11 # v4 + uses: actions/checkout@0ad4b8fadaa221de15dcec353f45205ec38ea70b # v4 - name: Install Nextflow - uses: nf-core/setup-nextflow@v1 + uses: nf-core/setup-nextflow@v2 - - uses: actions/setup-python@0a5c61591373683505ea898e09a3ea4f39ef2b9c # v5 + - uses: actions/setup-python@82c7e631bb3cdc910f68e0081d67478d79c6982d # v5 with: - python-version: "3.11" + python-version: "3.12" architecture: "x64" - name: Install dependencies @@ -60,7 +59,7 @@ jobs: - name: Upload linting log file artifact if: ${{ always() }} - uses: actions/upload-artifact@5d5d22a31266ced268874388b861e4b58bb5c2f3 # v4 + uses: actions/upload-artifact@65462800fd760344b1a7b4382951275a0abb4808 # v4 with: name: linting-logs path: | diff --git a/.github/workflows/linting_comment.yml b/.github/workflows/linting_comment.yml index b706875..40acc23 100644 --- a/.github/workflows/linting_comment.yml +++ b/.github/workflows/linting_comment.yml @@ -11,7 +11,7 @@ jobs: runs-on: ubuntu-latest steps: - name: Download lint results - uses: dawidd6/action-download-artifact@f6b0bace624032e30a85a8fd9c1a7f8f611f5737 # v3 + uses: dawidd6/action-download-artifact@09f2f74827fd3a8607589e5ad7f9398816f540fe # v3 with: workflow: linting.yml workflow_conclusion: completed From 75fea7f3c2a7560d30caf7221d01decd789252cb Mon Sep 17 00:00:00 2001 From: BertGalle Date: Wed, 12 Jun 2024 13:52:33 +0200 Subject: [PATCH 61/64] usage.md edit + changes to environments --- docs/usage.md | 18 +++++++++--------- .../local/exomedepth/cnv_call/environment.yml | 5 ++--- modules/local/exomedepth/count/environment.yml | 5 ++--- 3 files changed, 13 insertions(+), 15 deletions(-) diff --git a/docs/usage.md b/docs/usage.md index 8a01203..50b2400 100644 --- a/docs/usage.md +++ b/docs/usage.md @@ -29,15 +29,15 @@ Sample6,poolF,Fam5,/path/to/cram/Sample6,/path/to/crai/Sample6,/path/to/vcf/Samp ### All samplesheet options -| Column | Description | -| -------- | ----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- | --- | -| `sample` | Custom sample name. Cannot contain spaces and has to be unique. | -| `pool` | Pool name for the current sample. Samples sharing the same pool will be analysed together since CNV calling in the ExomeDepth workflow is executed per pool. It's recommended to put the (genetically speaking) female and male samples in seperate pools. The samples in the same pool should be enriched with the same library prep kit. An optimized aggregated reference set is chosen from the samples of the pool. Cannot contain spaces. | -| `family` | Family name for the current sample. Samples sharing this family name will be excluded from the aggregate reference set to ensure that common CNVs to this family will be detected. ExomeDepth assumes that the CNV of interest is absent from the aggregate reference set. Cannot contain spaces. | -| `cram` | Path to the CRAM (or BAM) file to be used by the pipeline for the current sample. | -| `crai` | Path to the CRAM (or BAI) index file. | -| `vcf` | Path to the VCF file to be used by the pipeline for the current sample. When this is provided, the pipeline will skip to the annotation. | -| `tbi` | Path to the TBI index file. | | +| Column | Description | | +| -------- | ----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- | --- | +| `sample` | Custom sample name. Cannot contain spaces and has to be unique. | | +| `pool` | Pool name for the current sample. Samples sharing the same pool will be merged together during the pipeline since CNV calling in the ExomeDepth workflow is executed per pool. Cannot contain spaces. | | +| `family` | Family name for the current sample. Samples sharing this family name will be excluded from the reference set to ensure that CNVs common to this family are not excluded. Cannot contain spaces. | | +| `cram` | Path to the CRAM (or BAM) file to be used by the pipeline for the current sample. | | +| `crai` | Path to the CRAM (or BAI) index file. | | +| `vcf` | Path to the VCF file to be used by the pipeline for the current sample. When this is provided, the pipeline will skip to the annotation. | | +| `tbi` | Path to the TBI index file. | | An [example samplesheet](../assets/samplesheet.csv) has been provided with the pipeline. diff --git a/modules/local/exomedepth/cnv_call/environment.yml b/modules/local/exomedepth/cnv_call/environment.yml index 01c804a..c94e9d0 100644 --- a/modules/local/exomedepth/cnv_call/environment.yml +++ b/modules/local/exomedepth/cnv_call/environment.yml @@ -5,6 +5,5 @@ channels: - defaults dependencies: - - r=3.6=r36_1003 - - r-optparse=1.6.4=r36h6115d3f_0 - - r-exomedepth:1.1.16--r43hfb3cda0_3 + - conda-forge::r-base=4.3.1 + - bioconda::r-exomedepth=1.1.16 diff --git a/modules/local/exomedepth/count/environment.yml b/modules/local/exomedepth/count/environment.yml index f092ff4..9385e3e 100644 --- a/modules/local/exomedepth/count/environment.yml +++ b/modules/local/exomedepth/count/environment.yml @@ -5,6 +5,5 @@ channels: - defaults dependencies: - - r=3.6=r36_1003 - - r-optparse=1.6.4=r36h6115d3f_0 - - r-exomedepth:1.1.16--r43hfb3cda0_3 + - conda-forge::r-base=4.3.1 + - bioconda::r-exomedepth=1.1.16 From 72e97adef17794e5dca1549fabb9d23c3f9dda25 Mon Sep 17 00:00:00 2001 From: BertGalle Date: Wed, 12 Jun 2024 16:06:23 +0200 Subject: [PATCH 62/64] Changed environment to conda-forge + removed awk from version --- modules/local/exomedepth/merge_cnv/environment.yml | 2 +- modules/local/exomedepth/merge_count/environment.yml | 2 +- modules/local/exomedepth/merge_count/main.nf | 2 -- 3 files changed, 2 insertions(+), 4 deletions(-) diff --git a/modules/local/exomedepth/merge_cnv/environment.yml b/modules/local/exomedepth/merge_cnv/environment.yml index 63d336e..e5265ed 100644 --- a/modules/local/exomedepth/merge_cnv/environment.yml +++ b/modules/local/exomedepth/merge_cnv/environment.yml @@ -4,4 +4,4 @@ channels: - bioconda - defaults dependencies: - - anaconda::coreutils=9.3 + - conda-forge::coreutils=9.3 diff --git a/modules/local/exomedepth/merge_count/environment.yml b/modules/local/exomedepth/merge_count/environment.yml index 63d336e..e5265ed 100644 --- a/modules/local/exomedepth/merge_count/environment.yml +++ b/modules/local/exomedepth/merge_count/environment.yml @@ -4,4 +4,4 @@ channels: - bioconda - defaults dependencies: - - anaconda::coreutils=9.3 + - conda-forge::coreutils=9.3 diff --git a/modules/local/exomedepth/merge_count/main.nf b/modules/local/exomedepth/merge_count/main.nf index e4613bb..8228a76 100644 --- a/modules/local/exomedepth/merge_count/main.nf +++ b/modules/local/exomedepth/merge_count/main.nf @@ -33,7 +33,6 @@ process COUNT_MERGE { paste: \$(paste --version | sed '1!d; s/paste (GNU coreutils) //') mv: \$(mv --version | sed '1!d; s/mv (GNU coreutils) //') cp: \$(cp --version | sed '1!d; s/cp (GNU coreutils) //') - awk: \$(awk -version 2>&1 | sed '1!d; s/(.*//') END_VERSIONS """ @@ -47,7 +46,6 @@ process COUNT_MERGE { paste: \$(paste --version | sed '1!d; s/paste (GNU coreutils) //') mv: \$(mv --version | sed '1!d; s/mv (GNU coreutils) //') cp: \$(cp --version | sed '1!d; s/cp (GNU coreutils) //') - awk: \$(awk -version 2>&1 | sed '1!d; s/(.*//') END_VERSIONS """ } From 6337b55fb863116a7a9c555794191adb4c45e694 Mon Sep 17 00:00:00 2001 From: torossee Date: Wed, 12 Jun 2024 16:56:16 +0200 Subject: [PATCH 63/64] bump-version 1.0.0 --- assets/multiqc_config.yml | 2 +- nextflow.config | 2 +- 2 files changed, 2 insertions(+), 2 deletions(-) diff --git a/assets/multiqc_config.yml b/assets/multiqc_config.yml index 5269074..e373493 100644 --- a/assets/multiqc_config.yml +++ b/assets/multiqc_config.yml @@ -1,5 +1,5 @@ report_comment: > - This report has been generated by the     nf-cmgg/exomecnv + This report has been generated by the nf-cmgg/exomecnv analysis pipeline. report_section_order: "nf-cmgg-exomecnv-methods-description": diff --git a/nextflow.config b/nextflow.config index d1de4cc..774134f 100644 --- a/nextflow.config +++ b/nextflow.config @@ -249,7 +249,7 @@ manifest { description = """A nextflow pipeline for calling exome CNVs""" mainScript = 'main.nf' nextflowVersion = '!>=23.04.0' - version = '1.0dev' + version = '1.0.0' doi = '' } From 94816ce36881473673fe505af68cf8383d1f4404 Mon Sep 17 00:00:00 2001 From: torossee Date: Wed, 12 Jun 2024 17:26:01 +0200 Subject: [PATCH 64/64] update changelog --- CHANGELOG.md | 10 ++++------ 1 file changed, 4 insertions(+), 6 deletions(-) diff --git a/CHANGELOG.md b/CHANGELOG.md index 885bf91..c324ad8 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -3,12 +3,10 @@ The format is based on [Keep a Changelog](https://keepachangelog.com/en/1.0.0/) and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0.html). -## v1.0dev - [date] +## v1.0.0 - 2024-06-12 -Initial release of nf-cmgg/exomecnv, created with the [nf-core](https://nf-co.re/) template. +Initial release of nf-cmgg/exomecnv, created with the [nf-core](https://nf-co.re/) template v2.13. -### `Changes` +### `Added` -- initiate step 1: CRAM -> BAM -- new repo name `nf-cmgg/exomecnv` -- Upgraded to nf-core v2.13 template +- First release of the pipeline - CNV calling using [ExomeDepth](https://github.com/vplagnol/ExomeDepth)
    Process Name \\", - " \\ Software Version
    CUSTOM_DUMPSOFTWAREVERSIONSpython3.11.7
    yaml5.4.1
    TOOL1tool10.11.9
    TOOL2tool21.9
    WorkflowNextflow