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INSTALL.md

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Mothur install instructions

Either download the precompiled binaries or compile from the source code. More detailed installation instructions are on the mothur wiki.

Download precompiled binaries

The easiest way to get mothur is to download the release from GitHub, unzip it, and you're ready to run Mothur.

Compile mothur from source

(For Unix-based operating systems.)

Download the mothur source code. Download the mothur tools external binaries.

Note: Tool version requirements - vsearch v2.15.2, uchime

Compiling with Boost:

1. Install dependencies.

You will need to install the following dependencies for Boost if not already on your machine:

  • bzip2
  • bzip2-devel
  • libz
  • zlib-devel

You can use a package manager such as yum, apt-get, homebrew, or conda.

2. Download Boost.

3. Follow their install instructions:

tar -xzvf boost_versionNumber.tar.gz
cd boost_versionNumber/
./bootstrap.sh --prefix=/desired/install/path
./b2 install

Alternatively, you can install boost on Linux with:

sudo apt-get install libboost-all-dev
sudo yum install libboost-all-dev

4. Compile mothur:

cd /path/to/mothur
make

If you get linking errors, it is likely because the zlib files were not found. You may need to add gzip.cpp and zlib.cpp to the source folder of mothur. They are located in boost_versionNumber/libs/iostreams/src/.

Compiling with HDF5:

1. Download and install HDF5.

tar -xzvf hdf5-1.10.3.tar.gz
cd hdf5-1.10.3
./configure --prefix=/desired/install/path --enable-cxx --enable-static --disable-shared
make check
make install

2. Edit the mothur makefile.

cd /path/to/mothur

Open the makefile in your preferred text editor:

vi Makefile

And edit the HDF5 filepaths:

HDF5_LIBRARY_DIR ?= "/path/to/hdf5/lib"
HDF5_INCLUDE_DIR ?= "/path/to/hdf5/include"

Save and close the makefile. (vi command :wq)

3. Compile mothur.

make