Welcome to porefile Discussions!

[iferres]

👋 Welcome!

We’re using Discussions as a place to:

• Ask questions about porefile that are not necessarily issues (bug reports, feature requests, etc).
• Discuss about long read metaprofiling.
• Share ideas.
• Engage with other community members.

[KFeye]

Hello! I accidentally went to the issue page (sorry kinda new) and I have a few questions (also this is super cool).

So, I am slightly obsessed with the idea of this analysis tool, so I apologize if I ask too many questions. I am trying to run the program (MacOS) and I have been using the barcode kit (96 expansion) and am trying very hard to get to a spot where I have taxa bar plots and diversity analysis.

I demultiplexed and trimmed in guppy. I have 96 folders with fastq.gz files (multiple per barcode). How would I read that data in and do I need to pre-process it any other way (so unzip everything and put it in one fast.gz file?? Also, does it make sense to go to google cloud? Is there a metadata file I need to create?

This is truly great work. Thank you!

[iferres]

For newcomers, following this at #11 😄