November 2021

[nvladimus]

💎 💎 💎 Autofocus has been added and works beautifully, outperforming expert human in focusing accuracy by 10x. Highly recommended!

💎 💎 Auto-optimization of ETL parameters is added, and works well most of the time, depending on samples.

💎 💎 Saving datasets as ImageJ TIFF files, including big ones (Fiji TIFF format that allows > 4 GB size). Voxel dimension saved in TIFF metadata.

💎 Button Freeze galvos is added to ETL tab for quick adjustment of ETL parameters outside of sample, see video tutorial.

✨ File Name Wizard auto-starts after Tiling Wizard.

✨ Mark All button is added to the Acquisition Manager

✨ Laser intensity can be edited directly via spinbox, alternative to slider. In Acquisition manager, slider is replaced by a spinbox for convenience.

✨ TIFF file name pattern for multi-tile/channel datasets is fully compatible with BigStitcher auto-loader, no renaming is needed.

🐛 fix: Going back to previously configured channel in the Tiling Manager appended a new channel to the acquisition list, rather than amending it.

🐛 fix: Files must always have extension (currently one of .tiff, .tif, .raw, .h5). Files without extension return an error.

🐛 fix: Image processing option generates MAX projections as TIFF files when output file format is either .raw or .tiff, #60.

⚠️ Recommended Python upgrade to 3.7 because some libraries have limited support for 3.6 (e.g. tifffile).

⚠️ Default file format for data saving has changed to .h5 for streamlined import into BigStitcher.

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This discussion was created from the release November 2021.