From 6ba5c39f49eb70234e073626a0c5345e927217c2 Mon Sep 17 00:00:00 2001 From: Ben Liesfeld Date: Sun, 1 Aug 2021 21:13:17 +0200 Subject: [PATCH] OPS-2945 reactivated IDT UDI 8nt config --- README.md | 16 +++++++++------- 1 file changed, 9 insertions(+), 7 deletions(-) diff --git a/README.md b/README.md index d6ddaf4..0dd974e 100644 --- a/README.md +++ b/README.md @@ -46,18 +46,20 @@ Then, restart IEM. ## Available index adapters -| Name | Manufacturer | Length/ nt | SamplePrepKit | IndexKits | -| ------------------------------------------------------------ | ------------------------------------------------------------ | ---------- | ---------------------- | ------------------------------------------------------------ | -| [IDX96](https://www.nimagen.com/shop/products/idx96-u01/index-primer-plate-and-nbsp-u01-96-unique-dual-indexes) | [Nimagen](https://www.nimagen.com/) | 10 | `Nimagen UDI` | `Nimagen IDX96-U01`
`Nimagen IDX96-U02v2`
`Nimagen IDX96-U03`
`Nimagen IDX96-U05`
`Nimagen IDX96-U06` | -| [IDX96](https://www.nimagen.com/shop/products/idx96-u01/index-primer-plate-and-nbsp-u01-96-unique-dual-indexes) | [Nimagen](https://www.nimagen.com/) | 8* | `Nimagen UDI` | `Nimagen IDX96-U01-8nt`
`Nimagen IDX96-U02v2-8nt`
`Nimagen IDX96-U03-8nt`
`Nimagen IDX96-U05-8nt`
`Nimagen IDX96-U06-8nt` | -| [IDX96](https://www.nimagen.com/shop/products/idx96-u01/index-primer-plate-and-nbsp-u01-96-unique-dual-indexes) | [Nimagen](https://www.nimagen.com/) | 8* | `Nimagen UDI` | `Nimagen IDX96-U01-RC-8nt`
`Nimagen IDX96-U02v2-RC-8nt`
`Nimagen IDX96-U03-RC-8nt`
`Nimagen IDX96-U05-RC-8nt`
`Nimagen IDX96-U06-RC-8nt` | -| [16-UDI](https://www.twistbioscience.com/resources/twist-universal-adapter-system) | [Twist Bioscience](https://www.twistbioscience.com/) | 10 | `Twist Enrichment UDI` | `Twist 16 UDI` | -| [96-UDI](https://www.twistbioscience.com/resources/twist-universal-adapter-system) | [Twist Bioscience](https://www.twistbioscience.com/) | 10 | `Twist Enrichment UDI` | `Twist 384 UDI`
`Twist 96 UDI Plate A`
`Twist 96 UDI Plate B`
`Twist 96 UDI Plate C`
`Twist 96 UDI Plate D` | +| Name | Manufacturer | Length/ nt | SamplePrepKit | IndexKits | +| ------------------------------------------------------------ | ---------------------------------------------------- | ---------- | ---------------------- | ------------------------------------------------------------ | +| [IDX96](https://www.nimagen.com/shop/products/idx96-u01/index-primer-plate-and-nbsp-u01-96-unique-dual-indexes) | [Nimagen](https://www.nimagen.com/) | 10 | `Nimagen UDI` | `Nimagen IDX96-U01`
`Nimagen IDX96-U02v2`
`Nimagen IDX96-U03`
`Nimagen IDX96-U05`
`Nimagen IDX96-U06` | +| [IDX96](https://www.nimagen.com/shop/products/idx96-u01/index-primer-plate-and-nbsp-u01-96-unique-dual-indexes) | [Nimagen](https://www.nimagen.com/) | 8* | `Nimagen UDI` | `Nimagen IDX96-U01-8nt`
`Nimagen IDX96-U02v2-8nt`
`Nimagen IDX96-U03-8nt`
`Nimagen IDX96-U05-8nt`
`Nimagen IDX96-U06-8nt` | +| [IDX96](https://www.nimagen.com/shop/products/idx96-u01/index-primer-plate-and-nbsp-u01-96-unique-dual-indexes) | [Nimagen](https://www.nimagen.com/) | 8* | `Nimagen UDI` | `Nimagen IDX96-U01-RC-8nt`
`Nimagen IDX96-U02v2-RC-8nt`
`Nimagen IDX96-U03-RC-8nt`
`Nimagen IDX96-U05-RC-8nt`
`Nimagen IDX96-U06-RC-8nt` | +| [16-UDI](https://www.twistbioscience.com/resources/twist-universal-adapter-system) | [Twist Bioscience](https://www.twistbioscience.com/) | 10 | `Twist Enrichment UDI` | `Twist 16 UDI` | +| [96-UDI](https://www.twistbioscience.com/resources/twist-universal-adapter-system) | [Twist Bioscience](https://www.twistbioscience.com/) | 10 | `Twist Enrichment UDI` | `Twist 384 UDI`
`Twist 96 UDI Plate A`
`Twist 96 UDI Plate B`
`Twist 96 UDI Plate C`
`Twist 96 UDI Plate D` | +| [IDT 8nt](https://eu.idtdna.com/pages/products/next-generation-sequencing/adapters/xgen-dual-index-umi-adapters-tech-access) | [IDT](https://eu.idtdna.com/pages) | 8 | `IDT UDI 8nt UMI` | `IDT UDI 8nt UMI Set A`
`IDT UDI 8nt UMI Set B`
`IDT UDI 8nt UMI Set C`
`IDT UDI 8nt UMI Set D`
`IDT UDI 8nt UMI 384 Indices` | *Some Nimagen amplicon designs require to use at least 151 cycles for Read 1 and Read 2. This is not compatible with 10nt indices on most devices. Nimagen states, however, that their 10nt index plates can be used as 8nt indices. For convenience, we created configurations with 8nt from those index plates. Please note that you must select different configurations depending on your device, for example: + * `Nimagen IDX96-U01-8nt` for the Forward Strand Workflow implemented in MiSeq and NovaSeq (v1.0 reagents), * `Nimagen IDX96-U01-RC-8nt` for the Reverse Complement Workflow implemented in NextSeq and NovaSeq (v1.5 reagents).