ImportError with Singularity

[aCoalBall]

Hi there,

Im trying to run in the singularity container. However it reports the following error

singularity exec --bind /usr/lib/locale/ /home/coalball/singularity_files/pepper_deepvariant-r0.8.simg run_pepper_margin_deepvariant call_variant -b dorado_sorted.bam -f /home/coalball/ref/ncbi-genomes-2023-05-19/GCF_009914755.1_T2T-CHM13v2.0_genomic.fna -o `pwd` -t 4 --ont_r9_guppy5_sup --sample_name HG001 -p PMDV_FINAL 2>&1 | tee pmdv.log 
[10-11-2023 07:23:18] INFO: VARIANT CALLING MODULE SELECTED
[10-11-2023 07:23:18] INFO: [1/9] RUNNING THE FOLLOWING COMMAND
-------
mkdir -p /home/coalball/projects/myNanoporeProject/data/HG001; 
mkdir -p /home/coalball/projects/myNanoporeProject/data/HG001/logs; 
mkdir -p /home/coalball/projects/myNanoporeProject/data/HG001/intermediate_files; 
cp /opt/pepper_models/PEPPER_VARIANT_ONT_R941_GUPPY5_SUP_V8.pkl /home/coalball/projects/myNanoporeProject/data/HG001/intermediate_files
-------
[10-11-2023 07:23:19] INFO: [2/9] RUNNING THE FOLLOWING COMMAND
-------
time pepper_variant call_variant -b dorado_sorted.bam -f /home/coalball/ref/ncbi-genomes-2023-05-19/GCF_009914755.1_T2T-CHM13v2.0_genomic.fna -t 4 -m /home/coalball/projects/myNanoporeProject/data/HG001/intermediate_files/PEPPER_VARIANT_ONT_R941_GUPPY5_SUP_V8.pkl -o /home/coalball/projects/myNanoporeProject/data/HG001/pepper/ --no_quantized  -s HG001 --ont_r9_guppy5_sup 2>&1 | tee /home/coalball/projects/myNanoporeProject/data/HG001/logs/1_pepper.log
-------
Traceback (most recent call last):
  File "/usr/local/bin/pepper_variant", line 5, in <module>
    from pepper_variant.pepper_variant import main
  File "/usr/local/lib/python3.8/dist-packages/pepper_variant/pepper_variant.py", line 11, in <module>
    from pepper_variant.modules.python.ImageGenerationUI import ImageGenerationUtils
  File "/usr/local/lib/python3.8/dist-packages/pepper_variant/modules/python/ImageGenerationUI.py", line 13, in <module>
    from pepper_variant.modules.python.AlignmentSummarizer import AlignmentSummarizer
  File "/usr/local/lib/python3.8/dist-packages/pepper_variant/modules/python/AlignmentSummarizer.py", line 3, in <module>
    from pysam import VariantFile
  File "/home/coalball/.local/lib/python3.8/site-packages/pysam/__init__.py", line 4, in <module>
    from pysam.libchtslib import *
ImportError: libcrypto.so.10: cannot open shared object file: No such file or directory

Could anyone tell me how to fix it?

Thanks