diff --git a/src/cards/__tests__/cards.test.tsx b/src/cards/__tests__/cards.test.tsx
index cb52a5102c..5d8b4bae0b 100644
--- a/src/cards/__tests__/cards.test.tsx
+++ b/src/cards/__tests__/cards.test.tsx
@@ -27,6 +27,7 @@ function findFooterPagination(wrapper: CardsWrapper): PaginationWrapper | null {
 
 const cardDefinition: CardsProps.CardDefinition<Item> = {
   header: item => `Header ${item.name}`,
+  testId: item => `card-${item.id}`,
   sections: [
     {
       id: 'description',
@@ -59,14 +60,19 @@ describe('Cards', () => {
   // index is 0-based
   const getCard = (index: number) => wrapper.findItems()[index];
 
-  const getCardHeader = (index: number) => getCard(index)?.findCardHeader()?.getElement();
+  const getCardByTestId = (testId: string) => wrapper.findItemByTestId(testId)!;
+
+  const getCardHeader = (index: number) => wrapper.findItems()[index]?.findCardHeader()?.getElement();
 
   // index is 0-based
-  const getCardsSections = (index: number) => getCard(index).findSections();
+  const getCardsSections = (index: number) => wrapper.findItems()[index].findSections();
 
   // cardIndex and sectionIndex are 0-based
   const getCardSection = (cardIndex: number, sectionIndex: number) => getCardsSections(cardIndex)[sectionIndex];
 
+  const getCardSectionByTestId = (cardIndex: number, sectionTestId: string) =>
+    getCard(cardIndex)!.findSectionByTestId(sectionTestId)!;
+
   const getCardSectionHeader = (cardIndex: number, sectionIndex: number) =>
     getCardSection(cardIndex, sectionIndex)?.findSectionHeader()?.getElement();
 
@@ -85,8 +91,7 @@ describe('Cards', () => {
       wrapper = renderCards(
         <Cards<Item> cardDefinition={cardDefinition} items={defaultItems} selectionType="single" />
       ).wrapper;
-
-      const cardsOrderedList = getCard(0).getElement().parentElement;
+      const cardsOrderedList = getCardByTestId('card-1').getElement().parentElement;
       expect(cardsOrderedList).toHaveAttribute('role', 'group');
     });
 
@@ -142,6 +147,7 @@ describe('Cards', () => {
             sections: [
               {
                 content: item => item.name,
+                testId: item => `card-${item.id}-content-section`,
               },
             ],
           }}
@@ -151,7 +157,8 @@ describe('Cards', () => {
       defaultItems.forEach((item, idx) => {
         expect(getCardsSections(idx)).toHaveLength(1);
 
-        expect(getCardSection(idx, 0).findSectionHeader()).toBe(null);
+        const sectionTestId = `card-${idx + 1}-content-section`;
+        expect(getCardSectionByTestId(idx, sectionTestId).findSectionHeader()).toBe(null);
         expect(getCardSectionContent(idx, 0)).toHaveTextContent(item.name);
       });
     });
@@ -176,6 +183,36 @@ describe('Cards', () => {
         expect(getCardSection(idx, 0)?.findContent()).toBe(null);
       });
     });
+
+    it('assigns test id attributes to the cards and sections', () => {
+      const { wrapper } = renderCards(
+        <Cards<Item>
+          cardDefinition={{
+            testId: item => `${item.name}-${item.id}`,
+            sections: [
+              {
+                testId: item => `${item.name}-${item.id}-id-section`,
+                header: 'id',
+              },
+              {
+                testId: item => `${item.name}-${item.id}-name-section`,
+                header: 'name',
+              },
+            ],
+          }}
+          items={defaultItems}
+        />
+      );
+
+      const itemTestIds = wrapper.findItems().map(item => item.getElement()!.getAttribute('data-testid'));
+      expect(itemTestIds).toEqual(['Apples-1', 'Oranges-2', 'Bananas-3']);
+
+      const secondItemSectionTestIds = wrapper
+        .findItems()[1]
+        .findSections()
+        .map(section => section.getElement()!.getAttribute('data-testid'));
+      expect(secondItemSectionTestIds).toEqual(['Oranges-2-id-section', 'Oranges-2-name-section']);
+    });
   });
 
   describe('header region', () => {
@@ -308,10 +345,85 @@ describe('Cards', () => {
           <Cards<Item> cardDefinition={cardDefinition} selectionType="multi" items={defaultItems} />
         </TestI18nProvider>
       ));
-      expect(getCard(0).findSelectionArea()!.getElement()).toHaveAttribute(
+      expect(getCardByTestId('card-1').findSelectionArea()!.getElement()).toHaveAttribute(
         'aria-label',
         expect.stringContaining('Custom label')
       );
     });
   });
+
+  describe('test utils', () => {
+    it('findItemByTestId returns the card by test id', () => {
+      const { wrapper } = renderCards(
+        <Cards<Item>
+          cardDefinition={{
+            testId: item => `card-${item.id}`,
+            sections: [
+              {
+                content: item => item.name,
+              },
+            ],
+          }}
+          items={defaultItems}
+        />
+      );
+
+      expect(wrapper.findItemByTestId('card-2')!.getElement()).toHaveTextContent('Orange');
+    });
+
+    it('findItemByTestId returns the card even if test id contains quotes', () => {
+      const { wrapper } = renderCards(
+        <Cards<Item>
+          cardDefinition={{
+            testId: item => `card-"${item.id}"`,
+          }}
+          items={defaultItems}
+        />
+      );
+
+      expect(wrapper.findItemByTestId('card-"2"')).toBeTruthy();
+    });
+
+    it('findSectionByTestId returns the section by test id', () => {
+      const { wrapper } = renderCards(
+        <Cards<Item>
+          cardDefinition={{
+            sections: [
+              {
+                content: item => `Item ID: ${item.id}`,
+                testId: item => `card-${item.id}-id`,
+              },
+              {
+                content: item => `Item Name: ${item.name}`,
+                testId: item => `card-${item.id}-name`,
+              },
+            ],
+          }}
+          items={defaultItems}
+        />
+      );
+
+      const firstItem = wrapper.findItems()[0]!;
+      expect(firstItem.findSectionByTestId('card-1-id')!.getElement()).toHaveTextContent('Item ID: 1');
+      expect(firstItem.findSectionByTestId('card-1-name')!.getElement()).toHaveTextContent('Item Name: Apples');
+    });
+
+    it('findSectionByTestId returns the section even if test id contains quotes', () => {
+      const { wrapper } = renderCards(
+        <Cards<Item>
+          cardDefinition={{
+            sections: [
+              {
+                testId: item => `card-section-"${item.id}"`,
+              },
+            ],
+          }}
+          items={defaultItems}
+        />
+      );
+
+      const firstItem = wrapper.findItems()[0]!;
+      expect(firstItem.findSectionByTestId('card-section-"1"')).toBeTruthy();
+    });
+  });
 });
diff --git a/src/cards/index.tsx b/src/cards/index.tsx
index 5dbecb0433..67cbcdd923 100644
--- a/src/cards/index.tsx
+++ b/src/cards/index.tsx
@@ -271,6 +271,7 @@ const CardsList = <T,>({
             [styles['card-selected']]: selectable && isItemSelected(item),
           })}
           key={getItemKey(trackBy, item, index)}
+          data-testid={cardDefinition.testId?.(item)}
           onFocus={onFocus}
           {...(focusMarkers && focusMarkers.item)}
           role={listItemRole}
@@ -301,8 +302,13 @@ const CardsList = <T,>({
                 </div>
               )}
             </div>
-            {visibleSectionsDefinition.map(({ width = 100, header, content, id }, index) => (
-              <div key={id || index} className={styles.section} style={{ width: `${width}%` }}>
+            {visibleSectionsDefinition.map(({ width = 100, header, content, id, testId }, index) => (
+              <div
+                key={id || index}
+                className={styles.section}
+                style={{ width: `${width}%` }}
+                data-testid={testId?.(item)}
+              >
                 {header ? <div className={styles['section-header']}>{header}</div> : ''}
                 {content ? <div className={styles['section-content']}>{content(item)}</div> : ''}
               </div>
diff --git a/src/cards/interfaces.tsx b/src/cards/interfaces.tsx
index 25b0066f3f..eb786fb2c0 100644
--- a/src/cards/interfaces.tsx
+++ b/src/cards/interfaces.tsx
@@ -223,6 +223,15 @@ export namespace CardsProps {
   export interface CardDefinition<T> {
     header?(item: T): React.ReactNode;
     sections?: ReadonlyArray<SectionDefinition<T>>;
+
+    /**
+     * Returns the test ID for each card item.
+     * Returned value is assigned to the `data-testid` attribute of the card's root element.
+     *
+     * @param {T} item Single item from the specified `items` array.
+     * @returns {string} Test id to be assigned to the corresponding card.
+     */
+    testId?(item: T): string;
   }
 
   export interface SectionDefinition<T> {
@@ -230,6 +239,15 @@ export namespace CardsProps {
     header?: React.ReactNode;
     content?(item: T): React.ReactNode;
     width?: number;
+
+    /**
+     * Returns the test ID for each section item.
+     * Returned value is assigned to the `data-testid` attribute of the section's root element.
+     *
+     * @param {T} item Single item from the specified `items` array.
+     * @returns {string} Test id to be assigned to the corresponding section.
+     */
+    testId?(item: T): string;
   }
 
   export interface CardsLayout {
diff --git a/src/test-utils/dom/cards/index.ts b/src/test-utils/dom/cards/index.ts
index 71b1afcb0b..3afc8b0936 100644
--- a/src/test-utils/dom/cards/index.ts
+++ b/src/test-utils/dom/cards/index.ts
@@ -1,6 +1,7 @@
 // Copyright Amazon.com, Inc. or its affiliates. All Rights Reserved.
 // SPDX-License-Identifier: Apache-2.0
 import { ComponentWrapper, ElementWrapper } from '@cloudscape-design/test-utils-core/dom';
+import { escapeSelector } from '@cloudscape-design/test-utils-core/utils';
 
 import CollectionPreferencesWrapper from '../collection-preferences';
 import ContainerWrapper from '../container';
@@ -30,6 +31,19 @@ export class CardWrapper extends ComponentWrapper {
     return this.findAllByClassName(styles.section).map(c => new CardSectionWrapper(c.getElement()));
   }
 
+  /**
+   * Returns the wrapper of the first card section that matches the specified test ID.
+   * Looks for the `data-testid` attribute that is assigned via `sections.testId` prop.
+   * If no matching card section is found, returns `null`.
+   *
+   * @param {string} testId
+   * @returns {CardSectionWrapper | null}
+   */
+  findSectionByTestId(testId: string): CardSectionWrapper | null {
+    const escapedTestId = escapeSelector(testId);
+    return this.findComponent(`.${styles.section}[data-testid="${escapedTestId}"]`, CardSectionWrapper);
+  }
+
   findCardHeader(): ElementWrapper | null {
     return this.findByClassName(styles['card-header-inner']);
   }
@@ -48,6 +62,19 @@ export default class CardsWrapper extends ComponentWrapper {
     return this.findAllByClassName(styles.card).map(c => new CardWrapper(c.getElement()));
   }
 
+  /**
+   * Returns the wrapper of the first card that matches the specified test ID.
+   * Looks for the `data-testid` attribute that is assigned via `cardDefinition.testId` prop.
+   * If no matching card is found, returns `null`.
+   *
+   * @param {string} testId
+   * @returns {CardWrapper | null}
+   */
+  findItemByTestId(testId: string): CardWrapper | null {
+    const escapedTestId = escapeSelector(testId);
+    return this.findComponent(`.${styles.card}[data-testid="${escapedTestId}"]`, CardWrapper);
+  }
+
   findSelectedItems(): Array<CardWrapper> {
     return this.findAllByClassName(styles['card-selected']).map(c => new CardWrapper(c.getElement()));
   }