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calib_15n.cw
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calib_15n.cw
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;15N 90 calibration based on HSQC sequence
;hsqcetfpf3gpsi2
;avance-version (07/04/04)
;HSQC
;2D H-1/X correlation via double inept transfer
; using sensitivity improvement
;phase sensitive using Echo/Antiecho-TPPI gradient selection
;with decoupling during acquisition
;using f3 - channel
;using flip-back pulse
;with gradients in back-inept
;
;A.G. Palmer III, J. Cavanagh, P.E. Wright & M. Rance, J. Magn.
; Reson. 93, 151-170 (1991)
;L.E. Kay, P. Keifer & T. Saarinen, J. Am. Chem. Soc. 114,
; 10663-5 (1992)
;J. Schleucher, M. Schwendinger, M. Sattler, P. Schmidt, O. Schedletzky,
; S.J. Glaser, O.W. Sorensen & C. Griesinger, J. Biomol. NMR 4,
; 301-306 (1994)
;S. Grzesiek & A. Bax, J. Am. Chem. Soc. 115, 12593-12594 (1993)
;
;$CLASS=HighRes
;$DIM=1D
;$TYPE=
;$SUBTYPE=
;$COMMENT=
prosol relations=<triple>
#include <Avance.incl>
#include <Grad.incl>
#include <Delay.incl>
"p2=p1*2"
"p22=p21*2"
"d11=30m"
"d24=1s/(92*4)"
"d26=1s/(92*4)"
"d0=3u"
;"in0=inf1/2"
"DELTA1=p16+d16+8u"
"DELTA2=d24-p19-d16"
"DELTA3=d26-p16-d16"
"acqt0=0"
# ifdef LABEL_CN
"DELTA=p16+d16+larger(p2,p8)+d0*2"
# else
"DELTA=p16+d16+p2+d0*2"
# endif /*LABEL_CN*/
1 ze
d11 pl16:f3
2 d1 do:f3
3 (p1 ph1)
d26 pl3:f3
(center (p2 ph2) (p22 ph6):f3 )
d26 UNBLKGRAD
(p1 ph2)
4u pl0:f1
(p11:sp1 ph1:r):f1
#ifdef CALIB_N
4u pl23:f3
(p23 ph3):f3
4u pl3:f3
#else
4u
#endif /*CALIB_N*/
p16:gp1
d16 pl1:f1
(p21 ph3):f3
d0
# ifdef LABEL_CN
(center (p2 ph7) (p8:sp13 ph1):f2 )
# else
(p2 ph7)
# endif /*LABEL_CN*/
d0
p16:gp2*EA
d16
(p22 ph4):f3
DELTA
(center (p1 ph1) (p21 ph4):f3 )
p19:gp4
d16
DELTA2
(center (p2 ph1) (p22 ph1):f3 )
DELTA2
p19:gp4
d16
(center (p1 ph2) (p21 ph5):f3 )
p16:gp5
d16
DELTA3
(center (p2 ph1) (p22 ph1):f3 )
DELTA3
p16:gp5
d16
(p1 ph1)
DELTA1
(p2 ph1)
4u
p16:gp3
d16 pl16:f3
4u BLKGRAD
go=2 ph31 cpd3:f3
30m do:f3 mc #0 to 2 F0(zd)
exit
ph1=0
ph2=1
ph3=0 2
ph4=0 0 2 2
ph5=1 1 3 3
ph6=0
ph7=0 0 2 2
ph31=2 0 0 2
;pl0 : 120dB
;pl1 : f1 channel - power level for pulse (default)
;pl3 : f3 channel - power level for pulse (default)
;pl23 : f3 channel - power level for calibration pulse (default)
;pl16: f3 channel - power level for CPD/BB decoupling
;sp1: f1 channel - shaped pulse 90 degree
;sp13: f2 channel - shaped pulse 180 degree (adiabatic)
;p1 : f1 channel - 90 degree high power pulse
;p2 : f1 channel - 180 degree high power pulse
;p8 : f2 channel - 180 degree shaped pulse for inversion (adiabatic)
;p11: f1 channel - 90 degree shaped pulse
;p16: homospoil/gradient pulse [1 msec]
;p19: gradient pulse 2 [500 usec]
;p21: f3 channel - 90 degree high power pulse
;p22: f3 channel - 180 degree high power pulse
;p23: f3 channel - 90 degree pulse for calibration
;d0 : incremented delay (2D) [3 usec]
;d1 : relaxation delay; 1-5 * T1
;d11: delay for disk I/O [30 msec]
;d16: delay for homospoil/gradient recovery
;d24: 1/(4J)YH for YH
; 1/(8J)YH for all multiplicities
;d26: 1/(4J(YH))
;cnst4: = J(YH)
;inf1: 1/SW(X) = 2 * DW(X)
;in0: 1/(2 * SW(X)) = DW(X)
;nd0: 2
;NS: 1 * n
;DS: >= 16
;td1: number of experiments
;FnMODE: echo-antiecho
;cpd3: decoupling according to sequence defined by cpdprg3
;pcpd3: f3 channel - 90 degree pulse for decoupling sequence
;use gradient ratio: gp 1 : gp 2 : gp 3 : gp 4 : gp 5
; 50 : 80 : 20.1 : 5 : -2 for C-13
; 50 : 80 : 8.1 : 5 : -2 for N-15
;for z-only gradients:
;gpz1: 50%
;gpz2: 80%
;gpz3: 8.1% for N-15, 20.1% for C-13
;gpz4: 5%
;gpz5: -2%
;use gradient files:
;gpnam1: SINE.100
;gpnam2: SINE.100
;gpnam3: SINE.100
;gpnam4: SINE.100
;gpnam5: SINE.100
;preprocessor-flags-start
;LABEL_CN: for C-13 and N-15 labeled samples start experiment with
; option -DLABEL_CN (eda: ZGOPTNS)
;preprocessor-flags-end
;$Id: hsqcetfpf3gpsi2,v 1.3 2007/04/11 13:34:30 ber Exp $