Background Fraction

[hk2rma]

How to interpret the background fraction column included in the metadata,
Does a value of 0.5 mean that 50% of the contents of that cell are ambient RNA?
I couldn't find any documentation on this and a HTML file was not generated.

Thanks

[bdferris642]

Also curious about this!

[seyoon-lee]

Adding on, I was wondering whether it would be plausible to remove cells with a high background fraction after running CellBender, or should I consider them ambient-free since CellBender would have already deducted the ambient reads?

[BornToBeJan]

How to interpret the background fraction column included in the metadata, Does a value of 0.5 mean that 50% of the contents of that cell are ambient RNA? I couldn't find any documentation on this and a HTML file was not generated.

Thanks

Any updates on this?