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allow configuration file to have resources per instrument type #106

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antgonza opened this issue Oct 10, 2023 · 7 comments
Open

allow configuration file to have resources per instrument type #106

antgonza opened this issue Oct 10, 2023 · 7 comments
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@antgonza
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iSeq processing uses way less resources than NovaSeq so it will be good to account for this while generating the compute resource allocations. In other words, we should allow for specific requests for iSeq via the config file.

Now, to id the instrument type, we need to use the letter after the first underscore in the runid; (from Gail) """e.g. 231005_A01535_0399_BHFNWNDSX7 where A01535 is the number of the instrument 01535, and A means NovaSeq
For: 20231009_FS10001773_73_BTR67718-2515, FS dentes iSeq and 0001773 is the machine number."""

@rob-knight
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rob-knight commented Oct 10, 2023 via email

@antgonza
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@rob-knight; after discussing the flow cell suggestion with Gail, we realized that flow cell type is not something currently being tracked but based on the machine id, it can be inferred.

@rob-knight
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rob-knight commented Oct 10, 2023 via email

@wasade
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wasade commented Oct 10, 2023

Looks like this info can be harvested from the xmls. I'm guessing the field is FlowCellMode but it would be good to confirm that, Kristin can likely verify quickly

(qiime2-2023.5) 13:31:14 (dtmcdonald@barnacle2):2023.10.10-gencode-v44$ grep -i flow /sequencing/igm_runs/231005_A01535_0399_BHFNWNDSX7/RunInfo.xml
		<Flowcell>HFNWNDSX7</Flowcell>
		<FlowcellLayout LaneCount="4" SurfaceCount="2" SwathCount="6" TileCount="78" FlowcellSide="2">
		</FlowcellLayout>
(qiime2-2023.5) 13:31:22 (dtmcdonald@barnacle2):2023.10.10-gencode-v44$ grep -i flow /sequencing/igm_runs/231005_A01535_0399_BHFNWNDSX7/RunParameters.xml
    <FlowCellSerialBarcode>HFNWNDSX7</FlowCellSerialBarcode>
    <FlowCellPartNumber>20015843</FlowCellPartNumber>
    <FlowCellLotNumber>20747361</FlowCellLotNumber>
    <FlowCellExpirationdate>04/13/2024 00:00:00</FlowCellExpirationdate>
    <FlowCellStartDate>10/05/2023 18:20:00</FlowCellStartDate>
    <FlowCellNumberOfReuseRemaining>1</FlowCellNumberOfReuseRemaining>
    <FlowCellSupportedModes>HTWashOnly;S4</FlowCellSupportedModes>
    <FlowCellMode>S4</FlowCellMode>
    <FlowCellConsumableVersion>1</FlowCellConsumableVersion>
    <FlowCellRssi>3</FlowCellRssi>
      <Board>Flow Cell Holder Board</Board>
  <WorkflowType>NovaSeqXp</WorkflowType>

@rob-knight
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rob-knight commented Oct 10, 2023 via email

@charles-cowart
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just an FYI I skimmed through the RunParameters.xml files for different sections of the /sequencing mount on barnacle and I noticed that FlowCellMode only appears in runs under igm_runs. Do we anticipate needing this value for other iseq and other runs as well?

@antgonza
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I think Jeff or Gail will know more about that ... can you send them a email directly? Thank you.

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