From 2d21fb87129a8f727aa8b4ced7e7582909358101 Mon Sep 17 00:00:00 2001 From: Jeffrey Barrick Date: Wed, 10 Nov 2021 20:40:04 -0600 Subject: [PATCH] Added warning for CDS features with lengths that are not multiples of 3, changed translation of truncated codons in these to a warning --- src/c/breseq/libbreseq/reference_sequence.h | 1 + src/c/breseq/reference_sequence.cpp | 68 +- tests/data/lambda/lambda_bad_orfs.gbk | 3280 +++++++++++++++++ .../expected.gd | 58 +- .../testcmd.sh | 2 +- 5 files changed, 3362 insertions(+), 47 deletions(-) create mode 100644 tests/data/lambda/lambda_bad_orfs.gbk rename tests/{lambda_polymorphism_no_junction => lambda_polymorphism_no_junction_bad_orfs}/expected.gd (84%) rename tests/{lambda_polymorphism_no_junction => lambda_polymorphism_no_junction_bad_orfs}/testcmd.sh (87%) diff --git a/src/c/breseq/libbreseq/reference_sequence.h b/src/c/breseq/libbreseq/reference_sequence.h index 2f1afce9..efe4d2f8 100644 --- a/src/c/breseq/libbreseq/reference_sequence.h +++ b/src/c/breseq/libbreseq/reference_sequence.h @@ -888,6 +888,7 @@ class cFeatureLocationList: public list { //!< Verify that all seq_id have sequence and that features fit in sequence; void VerifySequenceFeatureMatch(); + void VerifyCDSLengthsAreValid(); bool Initialized() {return m_initialized;} void ReadFASTA(const std::string &file_name); diff --git a/src/c/breseq/reference_sequence.cpp b/src/c/breseq/reference_sequence.cpp index 70cb1feb..97620a2e 100644 --- a/src/c/breseq/reference_sequence.cpp +++ b/src/c/breseq/reference_sequence.cpp @@ -825,6 +825,9 @@ namespace breseq { // Finally, update feature lists this->update_feature_lists(); + + // Check CDS lengths. Must be done after feature lists are updated + this->VerifyCDSLengthsAreValid(); } @@ -933,6 +936,31 @@ namespace breseq { if (this->empty()) ERROR("Reference files were not loaded"); } + // Warns about CDS features with out-of-frame lengths, consolidated into one message + void cReferenceSequences::VerifyCDSLengthsAreValid() + { + vector invalid_CDS_names; + for (vector::iterator itr= this->begin(); itr != this->end(); itr++) { + cAnnotatedSequence& as = *itr; + + for (cSequenceFeatureList::iterator itg = as.m_genes.begin(); itg != as.m_genes.end(); itg++ ) { + cGeneFeature gf = cGeneFeature(*(itg->get())); + + // Only check CDS features that do not have indeterminate ends + if ((gf.type == "CDS") && (!gf.m_start_is_indeterminate) && (!gf.m_end_is_indeterminate) ) { + string nt_seq = gf.get_nucleotide_sequence(as); + if (nt_seq.size() % 3 != 0) { + invalid_CDS_names.push_back(gf.get_locus_tag() + " (" + gf.name + ")"); + } + } + } + } + + if (invalid_CDS_names.size()>0) { + WARN("CDS feature(s) found with nucleotide length that are not a multiple of 3:\n" + join(invalid_CDS_names, ", ") + "\n\nTranslations of mutations in these genes may be incorrect.\nIt is recommended that you fix these feature annotations in your reference file!"); + } + } + void cReferenceSequences::ReadFASTA(const string &file_name) { cFastaFile ff(file_name, ios_base::in); @@ -2991,7 +3019,7 @@ void cReferenceSequences::annotate_1_mutation_in_genes(cDiffEntry& mut, vector(mutated_codon_number_1); aa_position = to_string(mutated_codon_number_1); - aa_ref_seq = translate_codon(codon_ref_seq, gene.translation_table, ( gene.start_is_indeterminate() && (mutated_codon_number_1 == 1) ) ? 2 : mutated_codon_number_1, gene.get_locus_tag()); - - // Generate mutated sequence - codon_new_seq = codon_ref_seq; - //#remember to revcom the change if gene is on opposite strand - codon_new_seq[mutated_codon_pos_1 - 1] = (mutated_strand == 1) ? mut[NEW_SEQ][0] : reverse_complement(mut[NEW_SEQ])[0]; - aa_new_seq = translate_codon(codon_new_seq, gene.translation_table, ( gene.start_is_indeterminate() && (mutated_codon_number_1 == 1) ) ? 2 : mutated_codon_number_1, gene.get_locus_tag()); - transl_table = to_string(gene.translation_table); - - if ((aa_ref_seq != "*") && (aa_new_seq == "*")) - snp_type = "nonsense"; - else if (aa_ref_seq != aa_new_seq) - snp_type = "nonsynonymous"; - else - snp_type = "synonymous"; - + if (codon_ref_seq.size() != 3) { + //>> Deal with the edge case of a mutation happening in the broken part of a truncated reading frame!! + WARN("Mutation in last codon of CDS feature with nucleotide length that is not a multiple of 3: " + gene.get_locus_tag() + " (" + gene.name + ").\nMutation is given no snp_type and will appear as a generic coding mutation.\nIt is recommended that you fix this feature annotation in your reference file!"); + gene_position = "coding (" + gene_position + "/" + gene_nt_size + " nt)"; + } else { + //>> Case of normal codon! + aa_ref_seq = translate_codon(codon_ref_seq, gene.translation_table, ( gene.start_is_indeterminate() && (mutated_codon_number_1 == 1) ) ? 2 : mutated_codon_number_1, gene.get_locus_tag()); + + // Generate mutated sequence + codon_new_seq = codon_ref_seq; + //#remember to revcom the change if gene is on opposite strand + codon_new_seq[mutated_codon_pos_1 - 1] = (mutated_strand == 1) ? mut[NEW_SEQ][0] : reverse_complement(mut[NEW_SEQ])[0]; + aa_new_seq = translate_codon(codon_new_seq, gene.translation_table, ( gene.start_is_indeterminate() && (mutated_codon_number_1 == 1) ) ? 2 : mutated_codon_number_1, gene.get_locus_tag()); + transl_table = to_string(gene.translation_table); + + if ((aa_ref_seq != "*") && (aa_new_seq == "*")) + snp_type = "nonsense"; + else if (aa_ref_seq != aa_new_seq) + snp_type = "nonsynonymous"; + else + snp_type = "synonymous"; + } } codon_position_is_indeterminate_list.push_back(codon_position_is_indeterminate); diff --git a/tests/data/lambda/lambda_bad_orfs.gbk b/tests/data/lambda/lambda_bad_orfs.gbk new file mode 100644 index 00000000..dc11c4f1 --- /dev/null +++ b/tests/data/lambda/lambda_bad_orfs.gbk @@ -0,0 +1,3280 @@ +LOCUS NC_001416 48502 bp DNA linear PHG 21-APR-2009 +DEFINITION Enterobacteria phage lambda, complete genome. +ACCESSION NC_001416 +VERSION NC_001416.1 GI:9626243 +DBLINK Project:14204 +KEYWORDS . +SOURCE Enterobacteria phage lambda + ORGANISM Enterobacteria phage lambda + Viruses; dsDNA viruses, no RNA stage; Caudovirales; Siphoviridae; + Lambda-like viruses. +REFERENCE 1 (sites) + AUTHORS Chen,C.Y. and Richardson,J.P. + TITLE Sequence elements essential for rho-dependent transcription + termination at lambda tR1 + JOURNAL J. Biol. Chem. 262 (23), 11292-11299 (1987) + PUBMED 3038914 +REFERENCE 2 (sites) + AUTHORS Peltz,S.W., Brown,A.L., Hasan,N., Podhajska,A.J. and Szybalski,W. + TITLE Thermosensitivity of a DNA recognition site: activity of a + truncated nutL antiterminator of coliphage lambda + JOURNAL Science 228 (4695), 91-93 (1985) + PUBMED 3156406 +REFERENCE 3 (sites) + AUTHORS Coleclough,C. and Erlitz,F.L. + TITLE Use of primer-restriction-end adapters in a novel cDNA cloning + strategy + JOURNAL Gene 34 (2-3), 305-314 (1985) + PUBMED 2408965 +REFERENCE 4 (sites) + AUTHORS Place,N., Fien,K., Mahoney,M.E., Wulff,D.L., Ho,Y.S., Debouck,C., + Rosenberg,M., Shih,M.C. and Gussin,G.N. + TITLE Mutations that alter the DNA binding site for the bacteriophage + lambda cII protein and affect the translation efficiency of the cII + gene + JOURNAL J. Mol. Biol. 180 (4), 865-880 (1984) + PUBMED 6241264 +REFERENCE 5 (sites) + AUTHORS Frackman,S., Siegele,D.A. and Feiss,M. + TITLE A functional domain of bacteriophage lambda terminase for prohead + binding + JOURNAL J. Mol. Biol. 180 (2), 283-300 (1984) + PUBMED 6096564 +REFERENCE 6 (sites) + AUTHORS Craig,N.L. and Nash,H.A. + TITLE E. coli integration host factor binds to specific sites in DNA + JOURNAL Cell 39 (3 PT 2), 707-716 (1984) + PUBMED 6096022 +REFERENCE 7 (sites) + AUTHORS Edlind,T.D., Cooley,T.E., Richards,S.H. and Ihler,G.M. + TITLE Long range base-pairing in the leftward transcription unit of + bacteriophage lambda. Characterization by electron microscopy and + computer-aided sequence analysis + JOURNAL J. Mol. Biol. 179 (3), 351-365 (1984) + PUBMED 6096550 +REFERENCE 8 (sites) + AUTHORS Warren,F. and Das,A. + TITLE Formation of termination-resistant transcription complex at phage + lambda nut locus: effects of altered translation and a ribosomal + mutation + JOURNAL Proc. Natl. Acad. Sci. U.S.A. 81 (12), 3612-3616 (1984) + PUBMED 6233610 +REFERENCE 9 (sites) + AUTHORS Wulff,D.L., Mahoney,M., Shatzman,A. and Rosenberg,M. + TITLE Mutational analysis of a regulatory region in bacteriophage lambda + that has overlapping signals for the initiation of transcription + and translation + JOURNAL Proc. Natl. Acad. Sci. U.S.A. 81 (2), 555-559 (1984) + PUBMED 6229793 +REFERENCE 10 (sites) + AUTHORS Hohn,B. + TITLE DNA sequences necessary for packaging of bacteriophage lambda DNA + JOURNAL Proc. Natl. Acad. Sci. U.S.A. 80 (24), 7456-7460 (1983) + PUBMED 6324174 +REFERENCE 11 (bases 45901 to 46443) + AUTHORS Taylor,A., Benedik,M. and Campbell,A. + TITLE Location of the Rz gene in bacteriophage lambda + JOURNAL Gene 26 (2-3), 159-163 (1983) + PUBMED 6323257 +REFERENCE 12 (bases 48469 to 48498) + AUTHORS Miwa,T. and Matsubara,K. + TITLE Lambda phage DNA sequences affecting the packaging process + JOURNAL Gene 24 (2-3), 199-206 (1983) + PUBMED 6227527 +REFERENCE 13 (bases 29063 to 29140) + AUTHORS Benedik,M., Mascarenhas,D. and Campbell,A. + TITLE The integrase promoter and T'I terminator in bacteriophages lambda + and 434 + JOURNAL Virology 126 (2), 658-668 (1983) + PUBMED 6305007 +REFERENCE 14 (bases 18414 to 18746) + AUTHORS Luk,K.C. and Szybalski,W. + TITLE A cluster of leftward, rho-dependent t'J terminators in the J gene + of coliphage lambda + JOURNAL Gene 21 (3), 175-191 (1983) + PUBMED 6221968 +REFERENCE 15 (bases 33000 to 33244; 33420 to 33543; 33629 to 34080) + AUTHORS Luk,K.C. and Szybalski,W. + TITLE The tL2 cluster of transcription termination sites between genes + bet and ral of coliphage lambda + JOURNAL Virology 125 (2), 403-418 (1983) + PUBMED 6220515 +REFERENCE 16 (bases 1 to 56; 48474 to 48502) + AUTHORS Feiss,M., Kobayashi,I. and Widner,W. + TITLE Separate sites for binding and nicking of bacteriophage lambda DNA + by terminase + JOURNAL Proc. Natl. Acad. Sci. U.S.A. 80 (4), 955-959 (1983) + PUBMED 6302676 +REFERENCE 17 (bases 33287 to 33486) + AUTHORS Knight,D.M. and Echols,H. + TITLE The cIII gene and protein of bacteriophage lambda + JOURNAL J. Mol. Biol. 163 (3), 505-510 (1983) + PUBMED 6220158 +REFERENCE 18 (bases 37938 to 38019) + AUTHORS Shih,M.C. and Gussin,G.N. + TITLE Mutations affecting two different steps in transcription initiation + at the phage lambda PRM promoter + JOURNAL Proc. Natl. Acad. Sci. U.S.A. 80 (2), 496-500 (1983) + PUBMED 6220405 +REFERENCE 19 (sites) + AUTHORS Daniels,D.L., Schroeder,J.L., Szybalski,W., Sanger,F. and + Blattner,F.R. + TITLE Appendix I: A molecular map of coliphage lambda + JOURNAL (in) Hendrix,R.W., Roberts,J.W., Stahl,F.W. and Weisberg,R.A. + (Eds.); + LAMBDA II: 469-517; + Cold Spring Harbor Laboratory, Cold Spring Harbor (1983) +REFERENCE 20 (sites) + AUTHORS Daniels,D.L., Schroeder,J.L., Szybalski,W., Sanger,F., + Coulson,A.R., Hong,G.F., Hill,D.F., Petersen,G.B. and Blattner,F.R. + TITLE Appendix II: Complete annotated lambda sequence + JOURNAL (in) Hendrix,R.W., Roberts,J.W., Stahl,F.W. and Weisberg,R.A. + (Eds.); + LAMBDA II: 519-674; + Cold Spring Harbor Laboratory, Cold Spring Harbor (1983) +REFERENCE 21 (bases 1 to 48502) + AUTHORS Sanger,F., Coulson,A.R., Hong,G.F., Hill,D.F. and Petersen,G.B. + TITLE Nucleotide sequence of bacteriophage lambda DNA + JOURNAL J. Mol. Biol. 162 (4), 729-773 (1982) + PUBMED 6221115 +REFERENCE 22 (bases 48424 to 48500) + AUTHORS Miwa,T. and Matsubara,K. + TITLE Identification of sequences necessary for packaging DNA into lambda + phage heads + JOURNAL Gene 20 (2), 267-279 (1982) + PUBMED 6299893 +REFERENCE 23 (bases 31299 to 31408) + AUTHORS Luk,K.C. and Szybalski,W. + TITLE Characterization of the cloned terminators tR1, tL3 and tI and the + nut R antitermination site of coliphage lambda + JOURNAL Gene 20 (2), 127-134 (1982) + PUBMED 6299882 +REFERENCE 24 (bases 40218 to 43972) + AUTHORS Kroger,M. and Hobom,G. + TITLE A chain of interlinked genes in the ninR region of bacteriophage + lambda + JOURNAL Gene 20 (1), 25-38 (1982) + PUBMED 6219042 +REFERENCE 25 (bases 35437 to 37348) + AUTHORS Landsmann,J., Kroger,M. and Hobom,G. + TITLE The rex region of bacteriophage lambda: two genes under three-way + control + JOURNAL Gene 20 (1), 11-24 (1982) + PUBMED 6219041 +REFERENCE 26 (bases 2521 to 3300) + AUTHORS Hong,G.F. + TITLE Sequencing of large double-stranded DNA using the dideoxy + sequencing technique + JOURNAL Biosci. Rep. 2 (11), 907-912 (1982) + PUBMED 6218841 +REFERENCE 27 (bases 38262 to 38386) + AUTHORS Lau,L.F., Roberts,J.W. and Wu,R. + TITLE Transcription terminates at lambda tR1 in three clusters + JOURNAL Proc. Natl. Acad. Sci. U.S.A. 79 (20), 6171-6175 (1982) + PUBMED 6216477 +REFERENCE 28 (bases 25157 to 27484) + AUTHORS Hong,G.F. + TITLE A systemic DNA sequencing strategy + JOURNAL J. Mol. Biol. 158 (3), 539-549 (1982) + PUBMED 6290669 +REFERENCE 29 (bases 37938 to 38018) + AUTHORS Hawley,D.K. and McClure,W.R. + TITLE Mechanism of activation of transcription initiation from the lambda + PRM promoter + JOURNAL J. Mol. Biol. 157 (3), 493-525 (1982) + PUBMED 6214638 +REFERENCE 30 (bases 31299 to 31408) + AUTHORS Luk,K.C. and Szybalski,W. + TITLE Transcription termination: sequence and function of the + rho-independent tL3 terminator in the major leftward operon of + bacteriophage lambda + JOURNAL Gene 17 (3), 247-258 (1982) + PUBMED 6213446 +REFERENCE 31 (bases 43682 to 45218) + AUTHORS Daniels,D.L. and Blattner,F.R. + TITLE Nucleotide sequence of the Q gene and the Q to S intergenic region + of bacteriophage lambda + JOURNAL Virology 117 (1), 81-92 (1982) + PUBMED 6461127 +REFERENCE 32 (bases 39219 to 39338) + AUTHORS Moore,D.D. and Blattner,F.R. + TITLE Sequence of lambda ric5b + JOURNAL J. Mol. Biol. 154 (1), 81-83 (1982) + PUBMED 6210782 +REFERENCE 33 (bases 27650 to 27741) + AUTHORS Kravchenko,V.V. and Mikryukov,N.N. + TITLE Localization of the promoter p-att of the binding site of + Escherichia coli polymerase on phage lambda DNA near the + integration site + JOURNAL Dokl. Biochem. 264, 148-151 (1982) +REFERENCE 34 (bases 35577 to 35647) + AUTHORS Hyman,H.C. and Honigman,A. + TITLE The use of the plasmid pHA10 in the isolation of lambda PL promoter + mutations + JOURNAL Mol. Gen. Genet. 185 (3), 515-517 (1982) + PUBMED 6285150 +REFERENCE 35 (bases 35468 to 35541) + AUTHORS Drahos,D. and Szybalski,W. + TITLE Antitermination and termination functions of the cloned nutL, N, + and tL1 modules of coliphage lambda + JOURNAL Gene 16 (1-3), 261-274 (1981) + PUBMED 6211393 +REFERENCE 36 (bases 43860 to 45001) + AUTHORS Petrov,N.A., Karginov,V.A., Mikriukov,N.N., Serpinski,O.I. and + Kravchenko,V.V. + TITLE Complete nucleotide sequence of the bacteriophage lambda DNA region + containing gene Q and promoter pR' + JOURNAL FEBS Lett. 133 (2), 316-320 (1981) + PUBMED 6458514 +REFERENCE 37 (bases 35468 to 35711) + AUTHORS Remaut,E., Stanssens,P. and Fiers,W. + TITLE Plasmid vectors for high-efficiency expression controlled by the PL + promoter of coliphage lambda + JOURNAL Gene 15 (1), 81-93 (1981) + PUBMED 6271633 +REFERENCE 38 (bases 32503 to 35905) + AUTHORS Ineichen,K., Shepherd,J.C. and Bickle,T.A. + TITLE The DNA sequence of the phage lambda genome between PL and the gene + bet + JOURNAL Nucleic Acids Res. 9 (18), 4639-4653 (1981) + PUBMED 6458018 +REFERENCE 39 (bases 38686 to 39224) + AUTHORS Moore,D.D., Denniston,K.J. and Blattner,F.R. + TITLE Sequence organization of the origins of DNA replication in lambdoid + coliphages + JOURNAL Gene 14 (1-2), 91-101 (1981) + PUBMED 6455332 +REFERENCE 40 (bases 35468 to 35819) + AUTHORS Horn,G.T. and Wells,R.D. + TITLE The leftward promoter of bacteriophage lambda. Isolation on a small + restriction fragment and deletion of adjacent regions + JOURNAL J. Biol. Chem. 256 (4), 1998-2002 (1981) + PUBMED 6257696 +REFERENCE 41 (bases 29055 to 29124) + AUTHORS Abraham,J. and Echols,H. + TITLE Regulation of int gene transcription by bacteriophage lambda. + Location of the RNA start generated by an int constitutive mutation + JOURNAL J. Mol. Biol. 146 (1), 157-165 (1981) + PUBMED 6455532 +REFERENCE 42 (bases 44972 to 45057) + AUTHORS Smith,G.R., Comb,M., Schultz,D.W., Daniels,D.L. and Blattner,F.R. + TITLE Nucleotide sequence of the chi recombinational hot spot chi +D in + bacteriophage lambda + JOURNAL J. Virol. 37 (1), 336-342 (1981) + PUBMED 6260986 +REFERENCE 43 (bases 29055 to 29131) + AUTHORS Miller,H.I., Abraham,J., Benedik,M., Campbell,A., Court,D., + Echols,H., Fischer,R., Galindo,J.M., Guarneros,G., Hernandez,T., + Mascarenhas,D., Montanez,C., Schindler,D., Schmeissner,U. and + Sosa,L. + TITLE Regulation of the integration-excision reaction by bacteriophage + lambda + JOURNAL Cold Spring Harb. Symp. Quant. Biol. 45 PT 1, 439-445 (1981) + PUBMED 6271488 +REFERENCE 44 (bases 43681 to 45634) + AUTHORS Daniels,D.L. + TITLE Control of late transcription in bacteriophage lambda + JOURNAL Thesis (1981) University of Wisconsin-Madison +REFERENCE 45 (bases 23131 to 23248) + AUTHORS Rosenvold,E.C., Calva,E., Burgess,R.R. and Szybalski,W. + TITLE In vitro transcription from the b2 region of bacteriophage lambda + JOURNAL Virology 107 (2), 476-487 (1980) + PUBMED 6450480 +REFERENCE 46 (bases 37305 to 37352) + AUTHORS Lieb,M. + TITLE IS5 increases recombination in adjacent regions as shown for the + repressor gene of coliphage lambda + JOURNAL Gene 12 (3-4), 277-280 (1980) + PUBMED 6265321 +REFERENCE 47 (bases 38102 to 38166) + AUTHORS Calva,E. and Burgess,R.R. + TITLE Characterization of a rho-dependent termination site within the cro + gene of bacteriophage lambda + JOURNAL J. Biol. Chem. 255 (22), 11017-11022 (1980) + PUBMED 6448859 +REFERENCE 48 (bases 37940 to 38016) + AUTHORS Rosen,E.D., Hartley,J.L., Matz,K., Nichols,B.P., Young,K.M., + Donelson,J.E. and Gussin,G.N. + TITLE DNA sequence analysis of prm-mutations of coliphage lambda + JOURNAL Gene 11 (3-4), 197-205 (1980) + PUBMED 6452305 +REFERENCE 49 (bases 27501 to 27615) + AUTHORS Mizuuchi,M. and Mizuuchi,K. + TITLE Integrative recombination of bacteriophage lambda: extent of the + DNA sequence involved in attachment site function + JOURNAL Proc. Natl. Acad. Sci. U.S.A. 77 (6), 3220-3224 (1980) + PUBMED 6251450 +REFERENCE 50 (bases 37940 to 38023) + AUTHORS Meyer,B.J., Maurer,R. and Ptashne,M. + TITLE Gene regulation at the right operator (OR) of bacteriophage lambda. + II. OR1, OR2, and OR3: their roles in mediating the effects of + repressor and cro + JOURNAL J. Mol. Biol. 139 (2), 163-194 (1980) + PUBMED 6447795 +REFERENCE 51 (bases 27479 to 27633) + AUTHORS Hsu,P.L., Ross,W. and Landy,A. + TITLE The lambda phage att site: functional limits and interaction with + Int protein + JOURNAL Nature 285 (5760), 85-91 (1980) + PUBMED 6246439 +REFERENCE 52 (bases 27724 to 29275) + AUTHORS Hoess,R.H., Foeller,C., Bidwell,K. and Landy,A. + TITLE Site-specific recombination functions of bacteriophage lambda: DNA + sequence of regulatory regions and overlapping structural genes for + Int and Xis + JOURNAL Proc. Natl. Acad. Sci. U.S.A. 77 (5), 2482-2486 (1980) + PUBMED 6446713 +REFERENCE 53 (bases 28929 to 29198) + AUTHORS Abraham,J., Mascarenhas,D., Fischer,R., Benedik,M., Campbell,A. and + Echols,H. + TITLE DNA sequence of regulatory region for integration gene of + bacteriophage lambda + JOURNAL Proc. Natl. Acad. Sci. U.S.A. 77 (5), 2477-2481 (1980) + PUBMED 6446712 +REFERENCE 54 (bases 27724 to 29525) + AUTHORS Davies,R.W. + TITLE DNA sequence of the int-xis-Pi region of the bacteriophage lambda; + overlap of the int and xis genes + JOURNAL Nucleic Acids Res. 8 (8), 1765-1782 (1980) + PUBMED 6253947 +REFERENCE 55 (bases 38237 to 38334) + AUTHORS Court,D., Brady,C., Rosenberg,M., Wulff,D.L., Behr,M., Mahoney,M. + and Izumi,S.U. + TITLE Control of transcription termination: a rho-dependent termination + site in bacteriophage lambda + JOURNAL J. Mol. Biol. 138 (2), 231-254 (1980) + PUBMED 6447791 +REFERENCE 56 (bases 38212 to 38467) + AUTHORS Wulff,D.L., Beher,M., Izumi,S., Beck,J., Mahoney,M., Shimatake,H., + Brady,C., Court,D. and Rosenberg,M. + TITLE Structure and function of the cy control region of bacteriophage + lambda + JOURNAL J. Mol. Biol. 138 (2), 209-230 (1980) + PUBMED 6447790 +REFERENCE 57 (bases 30493 to 30569) + AUTHORS Smith,G.R., Schultz,D.W. and Crasemann,J.M. + TITLE Generalized recombination: nucleotide sequence homology between Chi + recombinational hotspots + JOURNAL Cell 19 (3), 785-793 (1980) + PUBMED 6244897 +REFERENCE 58 (bases 36245 to 36343) + AUTHORS Pirrotta,V., Ineichen,K. and Walz,A. + TITLE An unusual RNA polymerase binding site in the immunity region of + phage lambda + JOURNAL Mol. Gen. Genet. 180 (2), 369-376 (1980) + PUBMED 6450873 +REFERENCE 59 (bases 37768 to 40293) + AUTHORS Schwarz,E., Scherer,G., Hobom,G. and Kossel,H. + TITLE The primary structure of the phage lambda P gene completes the + nucleotide sequence of the plasmid lambda-dvh93 + JOURNAL Biochem. Int. 1, 386-394 (1980) +REFERENCE 60 (bases 34957 to 35615) + AUTHORS Franklin,N.C. and Bennett,G.N. + TITLE The N protein of bacteriophage lambda, defined by its DNA sequence, + is highly basic + JOURNAL Gene 8 (1), 107-119 (1979) + PUBMED 43815 +REFERENCE 61 (bases 27711 to 27826) + AUTHORS Ross,W., Landy,A., Kikuchi,Y. and Nash,H. + TITLE Interaction of int protein with specific sites on lambda att DNA + JOURNAL Cell 18 (2), 297-307 (1979) + PUBMED 159130 +REFERENCE 62 (bases 38453 to 38500) + AUTHORS Smith,G.R., Faulds,D.H. and Sprague,K.U. + TITLE Nucleotide-sequence analysis of a chi site + JOURNAL Cold Spring Harb. Symp. Quant. Biol. 43 PT 2, 1067-1068 (1979) + PUBMED 158460 +REFERENCE 63 (bases 38470 to 39189) + AUTHORS Hobom,G., Grosschedl,R., Lusky,M., Scherer,G., Schwarz,E. and + Kossel,H. + TITLE Functional analysis of the replicator structure of lambdoid + bacteriophage DNAs + JOURNAL Cold Spring Harb. Symp. Quant. Biol. 43 PT 1, 165-178 (1979) + PUBMED 157835 +REFERENCE 64 (bases 38008 to 39328) + AUTHORS Moore,D.D., Denniston-Thompson,K., Kruger,K.E., Furth,M.E., + Williams,B.G., Daniels,D.L. and Blattner,F.R. + TITLE Dissection and comparative anatomy of the origins of replication of + lambdoid phages + JOURNAL Cold Spring Harb. Symp. Quant. Biol. 43 PT 1, 155-163 (1979) + PUBMED 157834 +REFERENCE 65 (bases 38453 to 38500) + AUTHORS Sprague,K.U., Faulds,D.H. and Smith,G.R. + TITLE A single base-pair change creates a Chi recombinational hotspot in + bacteriophage lambda + JOURNAL Proc. Natl. Acad. Sci. U.S.A. 75 (12), 6182-6186 (1978) + PUBMED 282634 +REFERENCE 66 (bases 37224 to 37940) + AUTHORS Sauer,R.T. + TITLE DNA sequence of the bacteriophage gama cI gene + JOURNAL Nature 276 (5685), 301-302 (1978) + PUBMED 714163 +REFERENCE 67 (bases 21661 to 31129) + AUTHORS Hoess,R.H. and Landy,A. + TITLE Structure of the lambda att sites generated by int-dependent + deletions + JOURNAL Proc. Natl. Acad. Sci. U.S.A. 75 (11), 5437-5441 (1978) + PUBMED 364480 +REFERENCE 68 (bases 35589 to 35666; 37938 to 38016) + AUTHORS Flashman,S.M. + TITLE Mutational analysis of the operators of bacteriophage lambda + JOURNAL Mol. Gen. Genet. 166 (1), 61-73 (1978) + PUBMED 368570 +REFERENCE 69 (bases 29711 to 29811; 31043 to 31058) + AUTHORS Davies,R.W., Schreier,P.H. and Buchel,D.E. + TITLE Determination of the endpoints of partial deletion mutants of the + attachment site of bacteriophage lambda by DNA sequencing + JOURNAL Nucleic Acids Res. 5 (9), 3209-3218 (1978) + PUBMED 704352 +REFERENCE 70 (bases 38597 to 39688) + AUTHORS Scherer,G. + TITLE Nucleotide sequence of the O gene and of the origin of replication + in bacteriophage lambda DNA + JOURNAL Nucleic Acids Res. 5 (9), 3141-3156 (1978) + PUBMED 704348 +REFERENCE 71 (bases 13 to 72; 48391 to 48502) + AUTHORS Nichols,B.P. and Donelson,J.E. + TITLE 178-Nucleotide sequence surrounding the cos site of bacteriophage + lambda DNA + JOURNAL J. Virol. 26 (2), 429-434 (1978) + PUBMED 666898 +REFERENCE 72 (bases 38212 to 38362) + AUTHORS Rosenberg,M., Court,D., Shimatake,H., Brady,C. and Wulff,D.L. + TITLE The relationship between function and DNA sequence in an + intercistronic regulatory region in phage lambda + JOURNAL Nature 272 (5652), 414-423 (1978) + PUBMED 634366 +REFERENCE 73 (bases 37990 to 38982) + AUTHORS Schwarz,E., Scherer,G., Hobom,G. and Kossel,H. + TITLE Nucleotide sequence of cro, cII and part of the O gene in phage + lambda DNA + JOURNAL Nature 272 (5652), 410-414 (1978) + PUBMED 264238 +REFERENCE 74 (sites) + AUTHORS Adhya,S. and Gottesman,M. + TITLE Control of transcription termination + JOURNAL Annu. Rev. Biochem. 47, 967-996 (1978) + PUBMED 354508 +REFERENCE 75 (bases 27616 to 28935) + AUTHORS Davies,R.W., Schreier,P.H. and Buchel,D.E. + TITLE Nucleotide sequence of the attachment site of coliphage lambda + JOURNAL Nature 270 (5639), 757-760 (1977) + PUBMED 593399 +REFERENCE 76 (bases 39062 to 39170) + AUTHORS Denniston-Thompson,K., Moore,D.D., Kruger,K.E., Furth,M.E. and + Blattner,F.R. + TITLE Physical structure of the replication origin of bacteriophage + lambda + JOURNAL Science 198 (4321), 1051-1056 (1977) + PUBMED 929187 +REFERENCE 77 (bases 38041 to 38241) + AUTHORS Roberts,T.M., Shimatake,H., Brady,C. and Rosenberg,M. + TITLE Sequence of Cro gene of bacteriophage lambda + JOURNAL Nature 270 (5634), 274-275 (1977) + PUBMED 593347 +REFERENCE 78 (bases 27617 to 27934) + AUTHORS Landy,A. and Ross,W. + TITLE Viral integration and excision: structure of the lambda att sites + JOURNAL Science 197 (4309), 1147-1160 (1977) + PUBMED 331474 +REFERENCE 79 (bases 37206 to 37263; 37914 to 37970) + AUTHORS Humayun,Z. + TITLE DNA sequence at the end of the cI gene in bacteriophage lambda + JOURNAL Nucleic Acids Res. 4 (7), 2137-2143 (1977) + PUBMED 909767 +REFERENCE 80 (bases 35578 to 35667) + AUTHORS Humayun,Z., Jeffrey,A. and Ptashne,M. + TITLE Completed DNA sequences and organization of repressor-binding sites + in the operators of phage lambda + JOURNAL J. Mol. Biol. 112 (2), 265-277 (1977) + PUBMED 875019 +REFERENCE 81 (bases 38610 to 38732) + AUTHORS Scherer,G., Hobom,G. and Kossel,H. + TITLE DNA base sequence of the po promoter region of phage lamdba + JOURNAL Nature 265 (5590), 117-121 (1977) + PUBMED 834253 +REFERENCE 82 (bases 44467 to 44807) + AUTHORS Sklar,J.L. + TITLE Structure and function of two regions of DNA controlling the + synthesis of prokaryotic RNAs + JOURNAL Thesis (1977) +REFERENCE 83 (bases 35578 to 35667; 37903 to 38027) + AUTHORS Ptashne,M., Backman,K., Humayun,M.Z., Jeffrey,A., Maurer,R., + Meyer,B. and Sauer,R.T. + TITLE Autoregulation and function of a repressor in bacteriophage lambda + JOURNAL Science 194 (4261), 156-161 (1976) + PUBMED 959843 +REFERENCE 84 (bases 37905 to 37989) + AUTHORS Walz,A., Pirrotta,V. and Ineichen,K. + TITLE Lambda repressor regulates the switch between PR and Prm promoters + JOURNAL Nature 262 (5570), 665-669 (1976) + PUBMED 958438 +REFERENCE 85 (bases 37946 to 38039) + AUTHORS Smith,G.R., Eisen,H., Reichardt,L. and Hedgepeth,J. + TITLE Deletions of lambda phage locating a prm mutation within the + rightward operator + JOURNAL Proc. Natl. Acad. Sci. U.S.A. 73 (3), 712-716 (1976) + PUBMED 1062780 +REFERENCE 86 (bases 35434 to 35618) + AUTHORS Dahlberg,J.E. and Blattner,F.R. + TITLE Sequence of the promoter-operator proximal region of the major + leftward RNA of bacteriophage lambda + JOURNAL Nucleic Acids Res. 2 (9), 1441-1458 (1975) + PUBMED 1178525 +REFERENCE 87 (bases 35583 to 35600) + AUTHORS Kleid,D.G., Agarwal,K.L. and Khorana,H.G. + TITLE The nucleotide sequence in the promoter region of the gene N in + bacteriophage lambda + JOURNAL J. Biol. Chem. 250 (14), 5574-5582 (1975) + PUBMED 167018 +REFERENCE 88 (bases 37945 to 38027) + AUTHORS Maniatis,T., Ptashne,M., Backman,K., Kield,D., Flashman,S., + Jeffrey,A. and Maurer,R. + TITLE Recognition sequences of repressor and polymerase in the operators + of bacteriophage lambda + JOURNAL Cell 5 (2), 109-113 (1975) + PUBMED 1095210 +REFERENCE 89 (bases 44588 to 44773) + AUTHORS Sklar,J., Yot,P. and Weissman,S.M. + TITLE Determination of genes, restriction sites, and DNA sequences + surrounding the 6S RNA template of bacteriophage lambda + JOURNAL Proc. Natl. Acad. Sci. U.S.A. 72 (5), 1817-1821 (1975) + PUBMED 1098044 +REFERENCE 90 (bases 37945 to 38018) + AUTHORS Maniatis,T., Jeffrey,A. and Kleid,D.G. + TITLE Nucleotide sequence of the rightward operator of phage lambda + JOURNAL Proc. Natl. Acad. Sci. U.S.A. 72 (3), 1184-1188 (1975) + PUBMED 1055375 +REFERENCE 91 (sites) + AUTHORS Weigel,P.H., Englund,P.T., Murray,K. and Old,R.W. + TITLE The 3'-terminal nucleotide sequences of bacteriophage lambda DNA + JOURNAL Proc. Natl. Acad. Sci. U.S.A. 70 (4), 1151-1155 (1973) + PUBMED 4515613 +REFERENCE 92 (bases 38597 to 38672) + AUTHORS Dahlberg,J.E. and Blattner,F.R. + TITLE In vitro transcription products of lambda DNA: Nucleotide sequences + and regulatory sites + JOURNAL (in) Fox,C.F. and Robinson,W.S. (Eds.); + VIRUS RESEARCH. PROCEEDINGS OF 1973 ICN-UCLA SYMPOSIUM: 533-544; + Academic Press, New York (1973) +REFERENCE 93 (bases 44588 to 44780) + AUTHORS Lebowitz,P., Weissman,S.M. and Radding,C.M. + TITLE Nucleotide sequence of a ribonucleic acid transcribed in vitro from + lambda phage deoxyribonucleic acid + JOURNAL J. Biol. Chem. 246 (16), 5120-5139 (1971) + PUBMED 4936723 +REFERENCE 94 (bases 1 to 12) + AUTHORS Wu,R. and Taylor,E. + TITLE Nucleotide sequence analysis of DNA. II. Complete nucleotide + sequence of the cohesive ends of bacteriophage lambda DNA + JOURNAL J. Mol. Biol. 57 (3), 491-511 (1971) + PUBMED 4931680 +REFERENCE 95 (bases 45493 to 45963) + AUTHORS Imada,M. and Tsugita,A. + TITLE Amino acid sequence of lambda phage endolysin + JOURNAL Nature New Biol. 233, 230-231 (1971) +REFERENCE 96 (bases 1 to 48502) + CONSRTM NCBI Genome Project + TITLE Direct Submission + JOURNAL Submitted (01-AUG-2000) National Center for Biotechnology + Information, NIH, Bethesda, MD 20894, USA +COMMENT PROVISIONAL REFSEQ: This record has not yet been subject to final + NCBI review. The reference sequence was derived from J02459. + [36] r-strand. + [72] fragments. + [(in) Hendrix,R.W., Roberts,J.W., Stahl,F.W. andWeisberg,R.A. + (Eds.);Lambda II: 4] review; complete genome. + [(in) Hendrix,R.W., Roberts,J.W., Stahl,F.W. and Weisberg, + R.A.(Eds.);Lambda II: 5] review; complete genome with annotation. + [2] both strands. + [4] sites; fragments at the 3'-terminus. + [24] comp strand. + [30] fragments. + [22] sites; transcription termination sites. + [84] sites; cohesive ends. + [91] sites; Pre-promoter mutations. + [87] sites; attP recombination site. + [88] sites; major leftward transcription unit. + [89] sites; prohead binding. + [90] sites; cII binding site mutations. + [92] sites; nutR mutations. + [93] sites; light chain oligonucleotides. + [94] sites; nutL antiterminator. + [95] sites; rho utilization sites A and B. + Contributed on tape by F.Sanger via D.L.Daniels. Most of references + [3] through [85] are either annotated by [(in) Hendrix,R.W., + Roberts,J.W., Stahl,F.W. andWeisberg,R.A. (Eds.);Lambda II: 4] and + [(in) Hendrix,R.W., Roberts,J.W., Stahl,F.W. and Weisberg, + R.A.(Eds.);Lambda II: 5], which are + the immediate sources for the annotation below, or they are cited + in Table 3 of [(in) Hendrix,R.W., Roberts,J.W., Stahl,F.W. + andWeisberg,R.A. (Eds.);Lambda II: 4]. Only references [27] through + [(in) Hendrix,R.W., Roberts,J.W., Stahl,F.W. and Weisberg, + R.A.(Eds.);Lambda II: 5] are represented + in the features table herein. + This is the best representation to date of the wild-type lambda + l-strand, though much of the sequence was determined for the + cI857s7 strain and changed to wild-type [(in) Hendrix,R.W., + Roberts,J.W., Stahl,F.W. andWeisberg,R.A. (Eds.);Lambda II: 4]. All + reported + variations leading to the strains cI857s7, imm21, imm434, lac5, + Nin5 and b2 are included in the annotation. The first twelve bases + are the sticky ends. A significant fraction of the known mutations + affecting replication and transcription have been annotated below; + a large number of point mutations, deletions and substitutions have + not. For a complete account of lambda mutations in relation to the + sequence, see [(in) Hendrix,R.W., Roberts,J.W., Stahl,F.W. + andWeisberg,R.A. (Eds.);Lambda II: 4]. + Each coding sequence belongs to a reading frame (orf) whose number, + given in parentheses, should indicate the number of amino acids + coded. The starting points for translation are known with varying + degrees of certainty; for example, the start site for the N + protein, given here as 35438, may turn out to be downstream (on the + complementary strand) at 35360. When direct empirical evidence such + as mutation or amino acid sequence is lacking, the start point is + said to be putative. For a summary of the evidence bearing upon the + coding sequences, see [72],[(in) Hendrix,R.W., Roberts,J.W., Stahl, + F.W. andWeisberg,R.A. (Eds.);Lambda II: 4]. Intergenic spaces in + lambda are + typically short and overlapping: the multiple reading frames (mult) + range between a span of 1 and a span of 103. In most cases, a start + codon precedes a termination codon, exceptions being the m-l + boundary (13429) and the 314-194 boundary (21973) which show the + E.coli trp operon pattern of 'translational coupling' (see + ). + Transcription in the central region, bases 22686 to 37940, is + leftward off the l-strand. In our annotation, this is indicated by + the letter 'c' and the descriptive term 'comp strand'. Signals and + recognition sites in this region, without judgement made about + their polarity, are treated accordingly, hence their span should be + read toward the left rather than toward the right. Furthermore some + leftward transcription is located outside the central region, and + that is also indicated by 'c' and 'comp strand'. In general, the + estimates for the extent or span of signals (e.g. operators), + binding sites (e.g. Nutr, int-binding sites, etc.) and of the + attachment site (att) vary in the literature. This annotation + follows [(in) Hendrix,R.W., Roberts,J.W., Stahl,F.W. andWeisberg, + R.A. (Eds.);Lambda II: 4]. No attempt is made to annotate promoters + as signals + because of the indefiniteness of their span, however known promoter + mutants are given. The cII protein is known to bind in the -35 + regions of p-i (29091) and pre(38369). Transcript termination sites + must be understood to be conditional on the N and Q proteins and + less than 100% efficient. There remain terminators to be found and + some of those annotated may have significance only in vitro. + COMPLETENESS: full length. +FEATURES Location/Qualifiers + source 1..48502 + /organism="Enterobacteria phage lambda" + /mol_type="genomic DNA" + /host="Escherichia coli" + /db_xref="taxon:10710" + gene 191..736 + /gene="nu1" + /locus_tag="lambdap01" + /db_xref="GeneID:2703523" + CDS 191..736 + /gene="nu1" + /locus_tag="lambdap01" + /codon_start=1 + /transl_table=11 + /product="DNA packaging protein" + /protein_id="NP_040580.1" + /db_xref="GI:9626244" + /db_xref="GeneID:2703523" + /translation="MEVNKKQLADIFGASIRTIQNWQEQGMPVLRGGGKGNEVLYDSA + AVIKWYAERDAEIENEKLRREVEELRQASEADLQPGTIEYERHRLTRAQADAQELKNA + RDSAEVVETAFCTFVLSRIAGEIASILDGLPLSVQRRFPELENRHVDFLKRDIIKAMN + KAAALDELIPGLLSEYIEQSG" + gene 711..2636 + /gene="A" + /locus_tag="lambdap02" + /db_xref="GeneID:2703524" + CDS 711..2636 + /gene="A" + /locus_tag="lambdap02" + /codon_start=1 + /transl_table=11 + /product="DNA packaging protein" + /protein_id="NP_040581.1" + /db_xref="GI:9626245" + /db_xref="GeneID:2703524" + /translation="MNISNSQVNRLRHFVRAGLRSLFRPEPQTAVEWADANYYLPKES + AYQEGRWETLPFQRAIMNAMGSDYIREVNVVKSARVGYSKMLLGVYAYFIEHKQRNTL + IWLPTDGDAENFMKTHVEPTIRDIPSLLALAPWYGKKHRDNTLTMKRFTNGRGFWCLG + GKAAKNYREKSVDVAGYDELAAFDDDIEQEGSPTFLGDKRIEGSVWPKSIRGSTPKVR + GTCQIERAASESPHFMRFHVACPHCGEEQYLKFGDKETPFGLKWTPDDPSSVFYLCEH + NACVIRQQELDFTDARYICEKTGIWTRDGILWFSSSGEEIEPPDSVTFHIWTAYSPFT + TWVQIVKDWMKTKGDTGKRKTFVNTTLGETWEAKIGERPDAEVMAERKEHYSAPVPDR + VAYLTAGIDSQLDRYEMRVWGWGPGEESWLIDRQIIMGRHDDEQTLLRVDEAINKTYT + RRNGAEMSISRICWDTGGIDPTIVYERSKKHGLFRVIPIKGASVYGKPVASMPRKRNK + NGVYLTEIGTDTAKEQIYNRFTLTPEGDEPLPGAVHFPNNPDIFDLTEAQQLTAEEQV + EKWVDGRKKILWDSKKRRNEALDCFVYALAALRISISRWQLDLSALLASLQEEDGAAT + NKKTLADYARALSGEDE" + gene 2633..2839 + /gene="W" + /locus_tag="lambdap03" + /db_xref="GeneID:2703525" + CDS 2633..2839 + /gene="W" + /locus_tag="lambdap03" + /codon_start=1 + /transl_table=11 + /product="head-tail joining protein" + /protein_id="NP_040582.1" + /db_xref="GI:9626246" + /db_xref="GeneID:2703525" + /translation="MTRQEELAAARAALHDLMTGKRVATVQKDGRRVEFTATSVSDLK + KYIAELEVQTGMTQRRRGPAGFYV" + gene 2836..4437 + /gene="B" + /locus_tag="lambdap04" + /db_xref="GeneID:2703526" + CDS 2836..4437 + /gene="B" + /locus_tag="lambdap04" + /codon_start=1 + /transl_table=11 + /product="capsid component" + /protein_id="NP_040583.1" + /db_xref="GI:9626247" + /db_xref="GeneID:2703526" + /translation="MKTPTIPTLLGPDGMTSLREYAGYHGGGSGFGGQLRSWNPPSES + VDAALLPNFTRGNARADDLVRNNGYAANAIQLHQDHIVGSFFRLSHRPSWRYLGIGEE + EARAFSREVEAAWKEFAEDDCCCIDVERKRTFTMMIREGVAMHAFNGELFVQATWDTS + SSRLFRTQFRMVSPKRISNPNNTGDSRNCRAGVQINDSGAALGYYVSEDGYPGWMPQK + WTWIPRELPGGRASFIHVFEPVEDGQTRGANVFYSVMEQMKMLDTLQNTQLQSAIVKA + MYAATIESELDTQSAMDFILGANSQEQRERLTGWIGEIAAYYAAAPVRLGGAKVPHLM + PGDSLNLQTAQDTDNGYSVFEQSLLRYIAAGLGVSYEQLSRNYAQMSYSTARASANES + WAYFMGRRKFVASRQASQMFLCWLEEAIVRRVVTLPSKARFSFQEARSAWGNCDWIGS + GRMAIDGLKEVQEAVMLIEAGLSTYEKECAKRGDDYQEIFAQQVRETMERRAAGLKPP + AWAAAAFESGLRQSTEEEKSDSRAA" + sig_peptide 2836..2901 + /gene="B" + /locus_tag="lambdap04" + /product="hypothetical protein" + /note="leader peptide" + mat_peptide 2902..4434 + /gene="B" + /locus_tag="lambdap04" + /product="processed B" + /protein_id="NP_955536.1" + /db_xref="GI:40795675" + gene 4418..5737 + /gene="C" + /locus_tag="lambdap05" + /db_xref="GeneID:2703527" + CDS 4418..5737 + /gene="C" + /locus_tag="lambdap05" + /codon_start=1 + /transl_table=11 + /product="capsid component" + /protein_id="NP_040584.1" + /db_xref="GI:9626248" + /db_xref="GeneID:2703527" + /translation="MTAELRNLPHIASMAFNEPLMLEPAYARVFFCALAGQLGISSLT + DAVSGDSLTAQEALATLALSGDDDGPRQARSYQVMNGIAVLPVSGTLVSRTRALQPYS + GMTGYNGIIARLQQAASDPMVDGILLDMDTPGGMVAGAFDCADIIARVRDIKPVWALA + NDMNCSAGQLLASAASRRLVTQTARTGSIGVMMAHSNYGAALEKQGVEITLIYSGSHK + VDGNPYSHLPDDVRETLQSRMDATRQMFAQKVSAYTGLSVQVVLDTEAAVYSGQEAID + AGLADELVNSTDAITVMRDALDARKSRLSGGRMTKETQSTTVSATASQADVTDVVPAT + EGENASAAQPDVNAQITAAVAAENSRIMGILNCEEAHGREEQARVLAETPGMTVKTAR + RILAAAPQSAQARSDTALDRLMQGAPAPLAAGNPASDAVNDLLNTPV" + gene 5132..5737 + /gene="nu3" + /locus_tag="lambdap06" + /db_xref="GeneID:2703528" + CDS 5132..5737 + /gene="nu3" + /locus_tag="lambdap06" + /codon_start=1 + /transl_table=11 + /product="capsid assembly protein" + /protein_id="NP_040585.1" + /db_xref="GI:9626249" + /db_xref="GeneID:2703528" + /translation="MDATRQMFAQKVSAYTGLSVQVVLDTEAAVYSGQEAIDAGLADE + LVNSTDAITVMRDALDARKSRLSGGRMTKETQSTTVSATASQADVTDVVPATEGENAS + AAQPDVNAQITAAVAAENSRIMGILNCEEAHGREEQARVLAETPGMTVKTARRILAAA + PQSAQARSDTALDRLMQGAPAPLAAGNPASDAVNDLLNTPV" + gene 5747..6079 + /gene="D" + /locus_tag="lambdap07" + /db_xref="GeneID:2703529" + CDS 5747..6079 + /gene="D" + /locus_tag="lambdap07" + /codon_start=1 + /transl_table=11 + /product="head-DNA stabilization protein" + /protein_id="NP_040586.1" + /db_xref="GI:9626250" + /db_xref="GeneID:2703529" + /translation="MTSKETFTHYQPQGNSDPAHTATAPGGLSAKAPAMTPLMLDTSS + RKLVAWDGTTDGAAVGILAVAADQTSTTLTFYKSGTFRYEDVLWPEAASDETKKRTAF + AGTAISIV" + gene 6135..7160 + /gene="E" + /locus_tag="lambdap08" + /db_xref="GeneID:2703482" + CDS 6135..7160 + /gene="E" + /locus_tag="lambdap08" + /codon_start=1 + /transl_table=11 + /product="capsid component" + /protein_id="NP_040587.1" + /db_xref="GI:9626251" + /db_xref="GeneID:2703482" + /translation="MSMYTTAQLLAANEQKFKFDPLFLRLFFRESYPFTTEKVYLSQI + PGLVNMALYVSPIVSGEVIRSRGGSTSEFTPGYVKPKHEVNPQMTLRRLPDEDPQNLA + DPAYRRRRIIMQNMRDEELAIAQVEEMQAVSAVLKGKYTMTGEAFDPVEVDMGRSEEN + NITQSGGTEWSKRDKSTYDPTDDIEAYALNASGVVNIIVFDPKGWALFRSFKAVKEKL + DTRRGSNSELETAVKDLGKAVSYKGMYGDVAIVVYSGQYVENGVKKNFLPDNTMVLGN + TQARGLRTYGCIQDADAQREGINASARYPKNWVTTGDPAREFTMIQSAPLMLLADPDE + FVSVQLA" + gene 7202..7600 + /gene="Fi" + /locus_tag="lambdap09" + /db_xref="GeneID:2703483" + CDS 7202..7600 + /gene="Fi" + /locus_tag="lambdap09" + /codon_start=1 + /transl_table=11 + /product="DNA packaging protein" + /protein_id="NP_040588.1" + /db_xref="GI:9626252" + /db_xref="GeneID:2703483" + /translation="MTKDELIARLRSLGEQLNRDVSLTGTKEELALRVAELKEELDDT + DETAGQDTPLSRENVLTGHENEVGSAQPDTVILDTSELVTVVALVKLHTDALHATRDE + PVAFVLPGTAFRVSAGVAAEMTERGLARMQ" + gene 7612..7965 + /gene="Fii" + /locus_tag="lambdap10" + /db_xref="GeneID:2703484" + CDS 7612..7965 + /gene="Fii" + /locus_tag="lambdap10" + /codon_start=1 + /transl_table=11 + /product="head-tail joining protein" + /protein_id="NP_040589.1" + /db_xref="GI:9626253" + /db_xref="GeneID:2703484" + /translation="MADFDNLFDAAIARADETIRGYMGTSATITSGEQSGAVIRGVFD + DPENISYAGQGVRVEGSSPSLFVRTDEVRQLRRGDTLTIGEENFWVDRVSPDDGGSCH + LWLGRGVPPAVNRRR" + gene 7977..8555 + /gene="Z" + /locus_tag="lambdap11" + /db_xref="GeneID:2703485" + CDS 7977..8555 + /gene="Z" + /locus_tag="lambdap11" + /codon_start=1 + /transl_table=11 + /product="tail component" + /protein_id="NP_040590.1" + /db_xref="GI:9626254" + /db_xref="GeneID:2703485" + /translation="MAIKGLEQAVENLSRISKTAVPGAAAMAINRVASSAISQSASQV + ARETKVRRKLVKERARLKRATVKNPQARIKVNRGDLPVIKLGNARVVLSRRRRRKKGQ + RSSLKGGGSVLVVGNRRIPGAFIQQLKNGRWHVMQRVAGKNRYPIDVVKIPMAVPLTT + AFKQNIERIRRERLPKELGYALQHQLRMVIKR" + gene 8552..8947 + /gene="U" + /locus_tag="lambdap12" + /db_xref="GeneID:2703486" + CDS 8552..8947 + /gene="U" + /locus_tag="lambdap12" + /codon_start=1 + /transl_table=11 + /product="tail component" + /protein_id="NP_040591.1" + /db_xref="GI:9626255" + /db_xref="GeneID:2703486" + /translation="MKHTELRAAVLDALEKHDTGATFFDGRPAVFDEADFPAVAVYLT + GAEYTGEELDSDTWQAELHIEVFLPAQVPDSELDAWMESRIYPVMSDIPALSDLITSM + VASGYDYRRDDDAGLWSSADLTYVITYEM" + gene 8955..9695 + /gene="V" + /locus_tag="lambdap13" + /db_xref="GeneID:2703487" + CDS 8955..9695 + /gene="V" + /locus_tag="lambdap13" + /codon_start=1 + /transl_table=11 + /product="tail component" + /protein_id="NP_040592.1" + /db_xref="GI:9626256" + /db_xref="GeneID:2703487" + /translation="MPVPNPTMPVKGAGTTLWVYKGSGDPYANPLSDVDWSRLAKVKD + LTPGELTAESYDDSYLDDEDADWTATGQGQKSAGDTSFTLAWMPGEQGQQALLAWFNE + GDTRAYKIRFPNGTVDVFRGWVSSIGKAVTAKEVITRTVKVTNVGRPSMAEDRSTVTA + ATGMTVTPASTSVVKGQSTTLTVAFQPEGVTDKSFRAVSADKTKATVSVSGMTITVNG + VAAGKVNIPVVSGNGEFAAVAEITVTAS" + gene 9711..10133 + /gene="G" + /locus_tag="lambdap14" + /db_xref="GeneID:2703488" + CDS 9711..10133 + /gene="G" + /locus_tag="lambdap14" + /codon_start=1 + /transl_table=11 + /product="tail component" + /protein_id="NP_040593.1" + /db_xref="GI:9626257" + /db_xref="GeneID:2703488" + /translation="MFLKTESFEHNGVTVTLSELSALQRIEHLALMKRQAEQAESDSN + RKFTVEDAIRTGAFLVAMSLWHNHPQKTQMPSMNEAVKQIEQEVLTTWPTEAISHAEN + VVYRLSGMYEFVVNNAPEQTEDAGPAEPVSAGKCSTVS" + gene 10115..10549 + /gene="T" + /locus_tag="lambdap15" + /db_xref="GeneID:2703489" + CDS 10115..10549 + /gene="T" + /locus_tag="lambdap15" + /codon_start=1 + /transl_table=11 + /product="tail component" + /protein_id="NP_040594.1" + /db_xref="GI:9626258" + /db_xref="GeneID:2703489" + /translation="MFDGELSFALKLAREMGRPDWRAMLAGMSSTEYADWHRFYSTHY + FHDVLLDMHFSGLTYTVLSLFFSDPDMHPLDFSLLNRREADEEPEDDVLMQKAAGLAG + GVRFGPDGNEVIPASPDVADMTEDDVMLMTVSEGIAGGVRYG" + gene 10542..13103 + /gene="H" + /locus_tag="lambdap16" + /db_xref="GeneID:2703511" + CDS 10542..13103 + /gene="H" + /locus_tag="lambdap16" + /codon_start=1 + /transl_table=11 + /product="tail component" + /protein_id="NP_040595.1" + /db_xref="GI:9626259" + /db_xref="GeneID:2703511" + /translation="MAEPVGDLVVDLSLDAARFDEQMARVRRHFSGTESDAKKTAAVV + EQSLSRQALAAQKAGISVGQYKAAMRMLPAQFTDVATQLAGGQSPWLILLQQGGQVKD + SFGGMIPMFRGLAGAITLPMVGATSLAVATGALAYAWYQGNSTLSDFNKTLVLSGNQA + GLTADRMLVLSRAGQAAGLTFNQTSESLSALVKAGVSGEAQIASISQSVARFSSASGV + EVDKVAEAFGKLTTDPTSGLTAMARQFHNVSAEQIAYVAQLQRSGDEAGALQAANEAA + TKGFDDQTRRLKENMGTLETWADRTARAFKSMWDAVLDIGRPDTAQEMLIKAEAAYKK + ADDIWNLRKDDYFVNDEARARYWDDREKARLALEAARKKAEQQTQQDKNAQQQSDTEA + SRLKYTEEAQKAYERLQTPLEKYTARQEELNKALKDGKILQADYNTLMAAAKKDYEAT + LKKPKQSSVKVSAGDRQEDSAHAALLTLQAELRTLEKHAGANEKISQQRRDLWKAESQ + FAVLEEAAQRRQLSAQEKSLLAHKDETLEYKRQLAALGDKVTYQERLNALAQQADKFA + QQQRAKRAAIDAKSRGLTDRQAEREATEQRLKEQYGDNPLALNNVMSEQKKTWAAEDQ + LRGNWMAGLKSGWSEWEESATDSMSQVKSAATQTFDGIAQNMAAMLTGSEQNWRSFTR + SVLSMMTEILLKQAMVGIVGSIGSAIGGAVGGGASASGGTAIQAAAAKFHFATGGFTG + TGGKYEPAGIVHRGEFVFTKEATSRIGVGNLYRLMRGYATGGYVGTPGSMADSRSQAS + GTFEQNNHVVINNDGTNGQIGPAALKAVYDMARKGARDEIQTQMRDGGLFSGGGR" + gene 13100..13429 + /gene="M" + /locus_tag="lambdap17" + /db_xref="GeneID:2703512" + CDS 13100..13429 + /gene="M" + /locus_tag="lambdap17" + /codon_start=1 + /transl_table=11 + /product="tail component" + /protein_id="NP_040596.1" + /db_xref="GI:9626260" + /db_xref="GeneID:2703512" + /translation="MKTFRWKVKPGMDVASVPSVRKVRFGDGYSQRAPAGLNANLKTY + SVTLSVPREEATVLESFLEEHGGWKSFLWTPPYEWRQIKVTCAKWSSRVSMLRVEFSA + EFEQVVN" + gene 13429..14127 + /gene="L" + /locus_tag="lambdap18" + /db_xref="GeneID:2703513" + CDS 13429..14127 + /gene="L" + /locus_tag="lambdap18" + /codon_start=1 + /transl_table=11 + /product="tail component" + /protein_id="NP_040597.1" + /db_xref="GI:9626261" + /db_xref="GeneID:2703513" + /translation="MQDIRQETLNECTRAEQSASVVLWEIDLTEVGGERYFFCNEQNE + KGEPVTWQGRQYQPYPIQGSGFELNGKGTSTRPTLTVSNLYGMVTGMAEDMQSLVGGT + VVRRKVYARFLDAVNFVNGNSYADPEQEVISRWRIEQCSELSAVSASFVLSTPTETDG + AVFPGRIMLANTCTWTYRGDECGYSGPAVADEYDQPTSDITKDKCSKCLSGCKFRNNV + GNFGGFLSINKLSQ" + gene 14276..14875 + /gene="K" + /locus_tag="lambdap19" + /db_xref="GeneID:2703514" + CDS 14276..14875 + /gene="K" + /locus_tag="lambdap19" + /codon_start=1 + /transl_table=11 + /product="tail component" + /protein_id="NP_040598.1" + /db_xref="GI:9626262" + /db_xref="GeneID:2703514" + /translation="MSPEDWLQAEMQGEIVALVHSHPGGLPWLSEADRRLQVQSDLPW + WLVCRGTIHKFRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGIEMPDFHREDDWWRNGQ + NLYLDNLEATGLYQVPLSAAQPGDVLLCCFGSSVPNHAAIYCGDGELLHHIPEQLSKR + ERYTDKWQRRTHSLWRHRAWRASAFTGIYNDLVAASTFV" + gene 14773..15444 + /gene="I" + /locus_tag="lambdap20" + /db_xref="GeneID:2703515" + CDS 14773..15444 + /gene="I" + /locus_tag="lambdap20" + /codon_start=1 + /transl_table=11 + /product="tail component" + /protein_id="NP_040599.1" + /db_xref="GI:9626263" + /db_xref="GeneID:2703515" + /translation="MAATHTLPLASPGMARICLYGDLQRFGRRIDLRVKTGAEAIRAL + ATQLPAFRQKLSDGWYQVRIAGRDVSTSGLTAQLHETLPDGAVIHIVPRVAGAKSGGV + FQIVLGAAAIAGSFFTAGATLAAWGAAIGAGGMTGILFSLGASMVLGGVAQMLAPKAR + TPRIQTTDNGKQNTYFSSLDNMVAQGNVLPVLYGEMRVGSRVVSQEISTADEGDGGQV + VVIGR" + gene 15505..18903 + /gene="J" + /locus_tag="lambdap21" + /db_xref="GeneID:2703516" + CDS 15505..18903 + /gene="J" + /locus_tag="lambdap21" + /codon_start=1 + /transl_table=11 + /product="tail:host specificity protein" + /protein_id="NP_040600.1" + /db_xref="GI:9626264" + /db_xref="GeneID:2703516" + /translation="MGKGSSKGHTPREAKDNLKSTQLLSVIDAISEGPIEGPVDGLKS + VLLNSTPVLDTEGNTNISGVTVVFRAGEQEQTPPEGFESSGSETVLGTEVKYDTPITR + TITSANIDRLRFTFGVQALVETTSKGDRNPSEVRLLVQIQRNGGWVTEKDITIKGKTT + SQYLASVVMGNLPPRPFNIRMRRMTPDSTTDQLQNKTLWSSYTEIIDVKQCYPNTALV + GVQVDSEQFGSQQVSRNYHLRGRILQVPSNYNPQTRQYSGIWDGTFKPAYSNNMAWCL + WDMLTHPRYGMGKRLGAADVDKWALYVIGQYCDQSVPDGFGGTEPRITCNAYLTTQRK + AWDVLSDFCSAMRCMPVWNGQTLTFVQDRPSDKTWTYNRSNVVMPDDGAPFRYSFSAL + KDRHNAVEVNWIDPNNGWETATELVEDTQAIARYGRNVTKMDAFGCTSRGQAHRAGLW + LIKTELLETQTVDFSVGAEGLRHVPGDVIEICDDDYAGISTGGRVLAVNSQTRTLTLD + REITLPSSGTALISLVDGSGNPVSVEVQSVTDGVKVKVSRVPDGVAEYSVWELKLPTL + RQRLFRCVSIRENDDGTYAITAVQHVPEKEAIVDNGAHFDGEQSGTVNGVTPPAVQHL + TAEVTADSGEYQVLARWDTPKVVKGVSFLLRLTVTADDGSERLVSTARTTETTYRFTQ + LALGNYRLTVRAVNAWGQQGDPASVSFRIAAPAAPSRIELTPGYFQITATPHLAVYDP + TVQFEFWFSEKQIADIRQVETSTRYLGTALYWIAASINIKPGHDYYFYIRSVNTVGKS + AFVEAVGRASDDAEGYLDFFKGKITESHLGKELLEKVELTEDNASRLEEFSKEWKDAS + DKWNAMWAVKIEQTKDGKHYVAGIGLSMEDTEEGKLSQFLVAANRIAFIDPANGNETP + MFVAQGNQIFMNDVFLKRLTAPTITSGGNPPAFSLTPDGKLTAKNADISGSVNANSGT + LSNVTIAENCTINGTLRAEKIVGDIVKAASAAFPRQRESSVDWPSGTRTVTVTDDHPF + DRQIVVLPLTFRGSKRTVSGRTTYSMCYLKVLMNGAVIYDGAANEAVQVFSRIVDMPA + GRGNVILTFTLTSTRHSADIPPYTFASDVQVMVIKKQALGISVV" + gene complement(18482..35582) + /locus_tag="lambdap22" + /db_xref="GeneID:2703502" + mRNA complement(18482..35582) + /locus_tag="lambdap22" + /product="mRNA-pl (alt.; via t'j4 terminator)" + /db_xref="GeneID:2703502" + gene complement(18597..35582) + /locus_tag="lambdap23" + /db_xref="GeneID:2703536" + mRNA complement(18597..35582) + /locus_tag="lambdap23" + /product="mRNA-pl (alt.; via t'j3 terminator)" + /db_xref="GeneID:2703536" + gene complement(18637..35582) + /locus_tag="lambdap24" + /db_xref="GeneID:2703468" + mRNA complement(18637..35582) + /locus_tag="lambdap24" + /product="mRNA-pl (alt.; via t'j2 terminator)" + /db_xref="GeneID:2703468" + gene complement(18671..35582) + /locus_tag="lambdap25" + /db_xref="GeneID:2703472" + mRNA complement(18671..35582) + /locus_tag="lambdap25" + /product="mRNA-pl (alt.; via t'j1 terminator)" + /db_xref="GeneID:2703472" + gene 18965..19585 + /gene="lom" + /locus_tag="lambdap26" + /db_xref="GeneID:2703517" + CDS 18965..19585 + /gene="lom" + /locus_tag="lambdap26" + /codon_start=1 + /transl_table=11 + /product="outer host membrane" + /protein_id="NP_040601.1" + /db_xref="GI:9626265" + /db_xref="GeneID:2703517" + /translation="MRNVCIAVAVFAALAVTVTPARAEGGHGTFTVGYFQVKPGTLPS + LSGGDTGVSHLKGINVKYRYELTDSVGVMASLGFAASKKSSTVMTGEDTFHYESLRGR + YVSVMAGPVLQISKQVSAYAMAGVAHSRWSGSTMDYRKTEITPGYMKETTTARDESAM + RHTSVAWSAGIQINPAASVVVDIAYEGSGSGDWRTDGFIVGVGYKF" + variation 19368..23278 + /locus_tag="lambdap25" + /note="lac5 substitution" + gene 19650..20855 + /gene="orf-401" + /locus_tag="lambdap27" + /db_xref="GeneID:2703518" + CDS 19650..20855 + /gene="orf-401" + /locus_tag="lambdap27" + /codon_start=1 + /transl_table=11 + /product="Tail fiber protein" + /protein_id="NP_040602.1" + /db_xref="GI:9626266" + /db_xref="GeneID:2703518" + /translation="MAVKISGVLKDGTGKPVQNCTIQLKARRNSTTVVVNTVGSENPD + EAGRYSMDVEYGQYSVILQVDGFPPSHAGTITVYEDSQPGTLNDFLCAMTEDDARPEV + LRRLELMVEEVARNASVVAQSTADAKKSAGDASASAAQVAALVTDATDSARAASTSAG + QAASSAQEASSGAEAASAKATEAEKSAAAAESSKNAAATSAGAAKTSETNAAASQQSA + ATSASTAATKASEAATSARDAVASKEAAKSSETNASSSAGRAASSATAAENSARAAKT + SETNARSSETAAERSASAAADAKTAAAGSASTASTKATEAAGSAVSASQSKSAAEAAA + IRAKNSAKRAEDIASAVALEDADTTRKGIVQLSSATNSTSETLAATPKAVKVVMDETN + RKAHWTVRH" + gene complement(20147..20767) + /gene="orf206b" + /locus_tag="lambdap90" + /db_xref="GeneID:3827061" + CDS complement(20147..20767) + /gene="orf206b" + /locus_tag="lambdap90" + /codon_start=1 + /transl_table=11 + /product="hypothetical protein" + /protein_id="NP_040603.1" + /db_xref="GI:9626267" + /db_xref="GeneID:3827061" + /translation="MLLVALLSCTIPFLVVSASSSATAEAISSARFAEFFARIAAASA + ALLLCDADTALPAASVAFVDAVDALPAAAVFASAAAEALRSAAVSDDLAFVSDVFAAL + AEFSAAVAEEAARPALDDAFVSDDFAASFEATASRAEVAASDAFVAAVEADVAADCCD + AAAFVSDVFAAPALVAAAFFEDSAAAALFSASVAFADAASAPEDAS" + gene 21029..21973 + /gene="orf-314" + /locus_tag="lambdap28" + /db_xref="GeneID:2703519" + CDS 21029..21973 + /gene="orf-314" + /locus_tag="lambdap28" + /codon_start=1 + /transl_table=11 + /product="Tail fiber" + /protein_id="NP_040604.1" + /db_xref="GI:9626268" + /db_xref="GeneID:2703519" + /translation="MTNALAGKQPKNATLTALAGLSTAKNKLPYFAENDAASLTELTQ + VGRDILAKNSVADVLEYLGAGENSAFPAGAPIPWPSDIVPSGYVLMQGQAFDKSAYPK + LAVAYPSGVLPDMRGWTIKGKPASGRAVLSQEQDGIKSHTHSASASGTDLGTKTTSSF + DYGTKTTGSFDYGTKSTNNTGAHAHSLSGSTGAAGAHAHTSGLRMNSSGWSQYGTATI + TGSLSTVKGTSTQGIAYLSKTDSQGSHSHSLSGTAVSAGAHAHTVGIGAHQHPVVIGA + HAHSFSIGSHGHTITVNAAGNAENTVKNIAFNYIVRLA" + variation 21737..>21737 + /gene="orf-314" + /locus_tag="lambdap28" + /note="b2 substitution terminating at the att site" + gene 21973..22557 + /gene="orf-194" + /locus_tag="lambdap29" + /db_xref="GeneID:2703503" + CDS 21973..22557 + /gene="orf-194" + /locus_tag="lambdap29" + /codon_start=1 + /transl_table=11 + /product="Putative fiber assembly protein" + /protein_id="NP_040605.1" + /db_xref="GI:9626269" + /db_xref="GeneID:2703503" + /translation="MAFRMSEQPRTIKIYNLLAGTNEFIGEGDAYIPPHTGLPANSTD + IAPPDIPAGFVAVFNSDEASWHLVEDHRGKTVYDVASGDALFISELGPLPENFTWLSP + GGEYQKWNGTAWVKDTEAEKLFRIREAEETKKSLMQVASEHIAPLQDAADLEIATKEE + TSLLEAWKKYRVLLNRVDTSTAPDIEWPAVPVME" + gene complement(22686..23918) + /gene="ea47" + /locus_tag="lambdap80" + /db_xref="GeneID:3827051" + CDS complement(22686..23918) + /gene="ea47" + /locus_tag="lambdap80" + /codon_start=1 + /transl_table=11 + /product="ea47" + /protein_id="NP_040606.1" + /db_xref="GI:9626270" + /db_xref="GeneID:3827051" + /translation="MTKKPWERRLKDLSHLLKCCIDTYFDPELFRLNLNQFLQTARTV + TFIIQKNKNQIIGYDIWYNNNVIEKWKNDPLMAWAKNSRNTIEKQGDLEMYSEAKATL + ISSYIEENDIEFITNESMLNIGIKKLVRLAQKKLPSYLTESSIIKSERRWVANTLKDY + ELLHALAIIYGRMYNCCNSLGIQINNPMGDDVISPTSFDSLFDEARRITYLKLKDYSI + SKLSFSMIQYDNKIIPEDIKERLKLVDKPKNITSTEELVDYTAKLAETTFLKDGYHIQ + TLIFYDKQFHPIDLINTTFEDQADKYIFWRYAADRAKITNAYGFIWISELWLRKASIY + SNKPIHTMPIIDERLQVIGIDSNNNQKCISWKIVRENEEKKPTLEISTADSKHDEKPY + FMRSVLKAIGGDVNTMNN" + gene complement(23231) + /locus_tag="lambdap30" + /db_xref="GeneID:2703520" + mRNA complement(<23231..23231) + /locus_tag="lambdap30" + /product="mRNA-pbl" + /db_xref="GeneID:2703520" + misc_recomb 24389..24390 + gene complement(24509..25399) + /gene="ea31" + /locus_tag="lambdap81" + /db_xref="GeneID:3827052" + CDS complement(24509..25399) + /gene="ea31" + /locus_tag="lambdap81" + /codon_start=1 + /transl_table=11 + /product="ea31" + /protein_id="NP_040607.1" + /db_xref="GI:9626271" + /db_xref="GeneID:3827052" + /translation="MKKLPLPARTYSEMLNKCSEGMMQINVRNNFITHFPTFLQKEQQ + YRILSSTGQLFTYDRTHPLEPTTLVVGNLTKVKLEKLYENNLRDKNKPARTYYDDMLV + SSGEKCPFCGDIGQTKNIDHFLPIAHYPEFSVMPINLVPSCRDCNMGEKGQVFAVDEV + HQAIHPYIDKDIFFREQWVYANFVSGTPGAISFYVECPANWRQEDKHRALHHFKLLNI + ANRYRLEAGKHLSEVITQRNSFVKVIRKYSSTATFQQLQSEFIEANLKPIIDLNDFPN + YWKRVMYQCLANSEDFFRGI" + gene complement(25396..26973) + /gene="ea59" + /locus_tag="lambdap82" + /db_xref="GeneID:3827053" + CDS complement(25396..26973) + /gene="ea59" + /locus_tag="lambdap82" + /codon_start=1 + /transl_table=11 + /product="ea59" + /protein_id="NP_040608.1" + /db_xref="GI:9626272" + /db_xref="GeneID:3827053" + /translation="MLEFSVIERGGYIPAVEKNKAFLRADGWNDYSFVTMFYLTVFDE + HGEKCDIGNVKIGFVGQKEEVSTYSLIDKKFSQLPEMFFSLGESIDYYVNLSKLSDGF + KHNLLKAIQDLVVWPNRLADIENESVLNTSLLRGVTLSEIHGQFARVLNGLPELSDFH + FSFNRKSAPGFSDLTIPFEVTVNSMPSTNIHAFIGRNGCGKTTILNGMIGAITNPENN + EYFFSENNRLIESRIPKGYFRSLVSVSFSAFDPFTPPKEQPDPAKGTQYFYIGLKNAA + SNSLKSLGDLRLEFISAFIGCMRVDRKRQLWLEAIKKLSSDENFSNMELISLISKYEE + LRRNEPQIQVDDDKFTKLFYDNIQKYLLRMSSGHAIVLFTITRLVDVVGEKSLVLFDE + PEVHLHPPLLSAFLRTLSDLLDARNGVAIIATHSPVVLQEVPKSCMWKVLRSREAINI + IRPDIETFGENLGVLTREVFLLEVTNSGYHHLLSQSVDSELSYETILKNYNGQIGLEG + RTVLKAMIMNRDEGKVQ" + variation 27537 + /locus_tag="lambdap25" + /note="t in sib3; c in wild-type" + /replace="t" + gene complement(27538..35582) + /gene="xis" + /locus_tag="lambdap31" + /db_xref="GeneID:2703469" + mRNA complement(27538..35582) + /gene="xis" + /locus_tag="lambdap31" + /product="mRNA-pl (alt.; via ti terminator)" + /db_xref="GeneID:2703469" + gene complement(27538..29065) + /locus_tag="lambdap32" + /db_xref="GeneID:2703464" + mRNA complement(27538..29065) + /locus_tag="lambdap32" + /product="mRNA int (integration; 356; via ti terminator)" + /db_xref="GeneID:2703464" + variation 27547 + /locus_tag="lambdap32" + /note="a in hef13; g in wild-type" + /replace="a" + variation 27568 + /locus_tag="lambdap32" + /note="a in sib2; c in wild-type" + variation 27573 + /locus_tag="lambdap32" + /note="t in sib1; g in wild-type" + misc_binding 27583..27602 + /bound_moiety="int 1" + misc_binding 27615..27634 + /bound_moiety="int 2" + misc_binding 27714..27747 + /bound_moiety="int 3" + misc_recomb 27724..27738 + /citation=[78] + gene complement(27812..28882) + /gene="int" + /locus_tag="lambdap33" + /db_xref="GeneID:2703470" + CDS complement(27812..28882) + /gene="int" + /locus_tag="lambdap33" + /codon_start=1 + /transl_table=11 + /product="integration protein" + /protein_id="NP_040609.1" + /db_xref="GI:9626273" + /db_xref="GeneID:2703470" + /translation="MGRRRSHERRDLPPNLYIRNNGYYCYRDPRTGKEFGLGRDRRIA + ITEAIQANIELFSGHKHKPLTARINSDNSVTLHSWLDRYEKILASRGIKQKTLINYMS + KIKAIRRGLPDAPLEDITTKEIAAMLNGYIDEGKAASAKLIRSTLSDAFREAIAEGHI + TTNHVAATRAAKSEVRRSRLTADEYLKIYQAAESSPCWLRLAMELAVVTGQRVGDLCE + MKWSDIVDGYLYVEQSKTGVKIAIPTALHIDALGISMKETLDKCKEILGGETIIASTR + REPLSSGTVSRYFMRARKASGLSFEGDPPTFHELRSLSARLYEKQISDKFAQHLLGHK + SDTMASQYRDDRGREWDKIEIK" + misc_binding 27814..27854 + /bound_moiety="int 4" + gene complement(28860..29078) + /gene="xis" + /locus_tag="lambdap34" + /db_xref="GeneID:2703504" + CDS complement(28860..29078) + /gene="xis" + /locus_tag="lambdap34" + /codon_start=1 + /transl_table=11 + /product="Excisionase" + /protein_id="NP_040610.1" + /db_xref="GI:9626274" + /db_xref="GeneID:2703504" + /translation="MYLTLQEWNARQRRPRSLETVRRWVRECRIFPPPVKDGREYLFH + ESAVKVDLNRPVTGGLLKRIRNGKKAKS" + variation 29063 + /gene="xis" + /locus_tag="lambdap34" + /note="a in xis am6; g in wild-type" + /replace="a" + gene complement(29118..29285) + /locus_tag="lambdap35" + /db_xref="GeneID:2703530" + CDS complement(29118..29285) + /locus_tag="lambdap35" + /note="Predicted by GeneMark" + /codon_start=1 + /transl_table=11 + /product="hypothetical protein" + /protein_id="NP_597778.1" + /db_xref="GI:19263393" + /db_xref="GeneID:2703530" + /translation="MHFRVTGEWNGEPFNRVIEAENINDCYDHWMIWAQIAHADVTNI + RIEELKEHQAA" + gene complement(29374..29655) + /gene="ea8.5" + /locus_tag="lambdap36" + /db_xref="GeneID:2703505" + CDS complement(29374..29655) + /gene="ea8.5" + /locus_tag="lambdap36" + /codon_start=1 + /transl_table=11 + /product="ea8.5" + /protein_id="NP_040611.1" + /db_xref="GI:9626275" + /db_xref="GeneID:2703505" + /translation="MSINELESEQKDWALSMLCRSGVLSPCRHHEGVYVDEGIDIESA + YKYSMKVYKSNEDKSPFCNVREMTDTVQNYYHEYGGNDTCPLCTKHIDD" + gene complement(29847..30395) + /gene="ea22" + /locus_tag="lambdap83" + /db_xref="GeneID:3827054" + CDS complement(29847..30395) + /gene="ea22" + /locus_tag="lambdap83" + /codon_start=1 + /transl_table=11 + /product="ea22" + /protein_id="NP_040612.1" + /db_xref="GI:9626276" + /db_xref="GeneID:3827054" + /translation="MSEINSQALREAAEQAMHDDWGFDADLFHELVTPSIVLELLDER + ERNQQYIKRRDQENEDIALTVGKLRVELETAKSKLNEQREYYEGVISDGSKRIAKLES + NEVREDGNQFLVVRHPGKTPVIKHCTGDLEEFLRQLIEQDPLVTIDIITHRYYGVGGQ + WVQDAGEYLHMMSDAGIRIKGE" + gene complement(30839..31024) + /gene="orf61" + /locus_tag="lambdap37" + /db_xref="GeneID:2703506" + CDS complement(30839..31024) + /gene="orf61" + /locus_tag="lambdap37" + /codon_start=1 + /transl_table=11 + /product="hypothetical protein" + /protein_id="NP_040613.1" + /db_xref="GI:9626277" + /db_xref="GeneID:2703506" + /translation="MRETRYDNHGMHFSGSGLHILCAYACRHGTCSMTPQQENALRSI + ARQANSEIKKSQTAVSG" + gene complement(31005..31196) + /gene="orf63" + /locus_tag="lambdap38" + /db_xref="GeneID:2703507" + CDS complement(31005..31196) + /gene="orf63" + /locus_tag="lambdap38" + /codon_start=1 + /transl_table=11 + /product="hypothetical protein" + /protein_id="NP_040614.1" + /db_xref="GI:9626278" + /db_xref="GeneID:2703507" + /translation="MHKASSVELRTSIEMAHSLAQIGIRFVPIPVETDEEFHTLAASL + SQKLEMMVAKAEADERNQV" + gene complement(31169..31351) + /gene="orf60a" + /locus_tag="lambdap39" + /db_xref="GeneID:2703508" + CDS complement(31169..31351) + /gene="orf60a" + /locus_tag="lambdap39" + /codon_start=1 + /transl_table=11 + /product="hypothetical protein" + /protein_id="NP_040615.1" + /db_xref="GI:9626279" + /db_xref="GeneID:2703508" + /translation="MTHPHDNIRVGAITFVYSVTKRGWVFPGLSVIRNPLKAQRLAEE + INNKRGAVCTKHLLLS" + gene complement(31262..35582) + /locus_tag="lambdap40" + /db_xref="GeneID:2703535" + mRNA complement(31262..35582) + /locus_tag="lambdap40" + /product="mRNA-pl (alt.; via tl3 terminator)" + /db_xref="GeneID:2703535" + misc_recomb 31266..31267 + gene complement(31348..32028) + /gene="exo" + /locus_tag="lambdap41" + /db_xref="GeneID:2703522" + CDS complement(31348..32028) + /gene="exo" + /locus_tag="lambdap41" + /codon_start=1 + /transl_table=11 + /product="exonuclease" + /protein_id="NP_040616.1" + /db_xref="GI:9626280" + /db_xref="GeneID:2703522" + /translation="MTPDIILQRTGIDVRAVEQGDDAWHKLRLGVITASEVHNVIAKP + RSGKKWPDMKMSYFHTLLAEVCTGVAPEVNAKALAWGKQYENDARTLFEFTSGVNVTE + SPIIYRDESMRTACSPDGLCSDGNGLELKCPFTSRDFMKFRLGGFEAIKSAYMAQVQY + SMWVTRKNAWYFANYDPRMKREGLHYVVIERDEKYMASFDEIVPEFIEKMDEALAEIG + FVFGEQWR" + gene complement(32025..32810) + /gene="bet" + /locus_tag="lambdap84" + /db_xref="GeneID:3827055" + CDS complement(32025..32810) + /gene="bet" + /locus_tag="lambdap84" + /codon_start=1 + /transl_table=11 + /product="bet" + /protein_id="NP_040617.1" + /db_xref="GI:9626281" + /db_xref="GeneID:3827055" + /translation="MSTALATLAGKLAERVGMDSVDPQELITTLRQTAFKGDASDAQF + IALLIVANQYGLNPWTKEIYAFPDKQNGIVPVVGVDGWSRIINENQQFDGMDFEQDNE + SCTCRIYRKDRNHPICVTEWMDECRREPFKTREGREITGPWQSHPKRMLRHKAMIQCA + RLAFGFAGIYDKDEAERIVENTAYTAERQPERDITPVNDETMQEINTLLIALDKTWDD + DLLPLCSQIFRRDIRASSELTQAEAVKALGFLKQKAAEQKVAA" + gene complement(32816..33232) + /gene="gam" + /locus_tag="lambdap42" + /db_xref="GeneID:2703509" + CDS complement(32816..33232) + /gene="gam" + /locus_tag="lambdap42" + /codon_start=1 + /transl_table=11 + /product="host-nuclease inhibitor protein Gam" + /protein_id="NP_040618.1" + /db_xref="GI:9626282" + /db_xref="GeneID:2703509" + /translation="MDINTETEIKQKHSLTPFPVFLISPAFRGRYFHSYFRSSAMNAY + YIQDRLEAQSWARHYQQLAREEKEAELADDMEKGLPQHLFESLCIDHLQRHGASKKSI + TRAFDDDVEFQERMAEHIRYMVETIAHHQVDIDSEV" + gene complement(33100..35582) + /locus_tag="lambdap43" + /db_xref="GeneID:2703542" + mRNA complement(33100..35582) + /locus_tag="lambdap43" + /product="mRNA-pl (alt.; via tl2d terminator)" + /db_xref="GeneID:2703542" + gene complement(33141..35582) + /locus_tag="lambdap44" + /db_xref="GeneID:2703471" + mRNA complement(33141..35582) + /locus_tag="lambdap44" + /product="mRNA-pl (alt.; via tl2c terminator)" + /db_xref="GeneID:2703471" + gene complement(33187..33330) + /gene="kil" + /locus_tag="lambdap85" + /db_xref="GeneID:3827057" + CDS complement(33187..33330) + /gene="kil" + /locus_tag="lambdap85" + /codon_start=1 + /transl_table=11 + /product="host-killing protein" + /protein_id="NP_040619.1" + /db_xref="GI:9626283" + /db_xref="GeneID:3827057" + /translation="MDQTLMAIQTKFTIATFIGDEKMFREAVDAYKKWILILKLRSSK + SIH" + gene complement(33299..33463) + /gene="cIII" + /locus_tag="lambdap86" + /db_xref="GeneID:3827056" + CDS complement(33299..33463) + /gene="cIII" + /locus_tag="lambdap86" + /codon_start=1 + /transl_table=11 + /product="antitermination protein" + /protein_id="NP_040620.1" + /db_xref="GI:9626284" + /db_xref="GeneID:3827056" + /translation="MQYAIAGWPVAGCPSESLLERITRKLRDGWKRLIDILNQPGVPK + NGSNTYGYPD" + gene complement(33494..35582) + /gene="ea10" + /locus_tag="lambdap45" + /db_xref="GeneID:2703541" + mRNA complement(33494..35582) + /gene="ea10" + /locus_tag="lambdap45" + /product="mRNA-pl (alt.; via tl2b terminator)" + /db_xref="GeneID:2703541" + CDS complement(33536..33904) + /gene="ea10" + /locus_tag="lambdap45" + /codon_start=1 + /transl_table=11 + /product="Putative single-stranded DNA binding protein" + /protein_id="NP_040621.1" + /db_xref="GI:9626285" + /db_xref="GeneID:2703541" + /translation="MSNIKKYIIDYDWKASIEIEIDHDVMTEEKLHQINNFWSDSEYR + LNKHGSVLNAVLIMLAQHALLIAISSDLNAYGVVCEFDWNDGNGQEGWPPMDGSEGIR + ITDIDTSGIFDSDDMTIKAA" + gene complement(33930..35582) + /gene="ral" + /locus_tag="lambdap46" + /db_xref="GeneID:2703473" + mRNA complement(33930..35582) + /gene="ral" + /locus_tag="lambdap46" + /product="mRNA-pl (alt.; via tl2a terminator)" + /db_xref="GeneID:2703473" + CDS complement(34087..34287) + /gene="ral" + /locus_tag="lambdap46" + /codon_start=1 + /transl_table=11 + /product="restriction alleviation protein" + /protein_id="NP_040622.1" + /db_xref="GI:9626286" + /db_xref="GeneID:2703473" + /translation="MTTTIDKNQWCGQFKRCNGCKLQSECMVKPEEMFPVMEDGKYVD + KWAIRTTAMIARELGKQNNKAA" + gene complement(34271..34357) + /gene="orf28" + /locus_tag="lambdap47" + /db_xref="GeneID:2703510" + CDS complement(34271..34357) + /gene="orf28" + /locus_tag="lambdap47" + /codon_start=1 + /transl_table=11 + /product="hypothetical protein" + /protein_id="NP_040623.1" + /db_xref="GI:9626287" + /db_xref="GeneID:2703510" + /translation="MEEEFEEFEEHPQDVMEQYQDYPYDYDY" + variation 34378..38617 + /note="imm21 region" + gene 34482..35036 + /locus_tag="lambdap48" + /db_xref="GeneID:2703531" + CDS 34482..35036 + /locus_tag="lambdap48" + /note="Predicted by GeneMark" + /codon_start=1 + /transl_table=11 + /product="Superinfection exclusion protein B" + /protein_id="NP_597779.1" + /db_xref="GI:19263394" + /db_xref="GeneID:2703531" + /translation="MMSIEMDPLVILGRVFSNEPLERTMYMIVIWVGLLLLSPDNWPE + YVNERIGIPHVWHVFVFALAFSLAINVHRLSAIASARYKRFKLRKRIKMQNDKVRSVI + QNLTEEQSMVLCAALNEGRKYVVTSKQFPYISELIELGVLNKTFSRWNGKHILFPIED + IYWTELVASYDPYNIEIKPRPISK" + gene complement(34560..35582) + /gene="N" + /locus_tag="lambdap49" + /db_xref="GeneID:2703540" + mRNA complement(34560..35582) + /gene="N" + /locus_tag="lambdap49" + /product="mRNA-pl (alt.; via tl1 terminator)" + /db_xref="GeneID:2703540" + CDS complement(35037..35438) + /gene="N" + /locus_tag="lambdap49" + /codon_start=1 + /transl_table=11 + /product="early gene regulator" + /protein_id="NP_040625.1" + /db_xref="GI:9626289" + /db_xref="GeneID:2703540" + /translation="MCQSRGVFVQDYNCHTPPKLTDRRIQMDAQTRRRERRAEKQAQW + KAANPLLVGVSAKPVNRPILSLNRKPKSRVESALNPIDLTVLAEYHKQIESNLQRIER + KNQRTWYSKPGERGITCSGRQKIKGKSIPLI" + misc_binding complement(35518..35534) + /gene="N" + /locus_tag="lambdap49" + /note="N-utilization leftward.; putative" + /bound_moiety="Nutl" + variation 35528 + /gene="N" + /locus_tag="lambdap49" + /note="a in Nutl63; c in wild-type" + /replace="a" + variation 35528 + /gene="N" + /locus_tag="lambdap49" + /note="g in Nutl96; c in wild-type" + /replace="g" + variation 35528 + /gene="N" + /locus_tag="lambdap49" + /note="t in Nutl18; c in wild-type" + /replace="t" + variation 35529..35531 + /gene="N" + /locus_tag="lambdap49" + /note="agg in wild-type; ag in Nutl3" + /replace="ag" + variation 35583..38245 + /note="imm434 region" + misc_signal complement(35591..35607) + /note="operator-l1 (first base on comp strand)" + variation 35596 + /note="a in vir2, t in v003, c in wild-type" + variation 35606 + /note="c in vir101; t in wild-type" + /replace="c" + misc_signal complement(35615..35631) + /note="operator-l2 (first base on comp strand)" + variation 35621 + /note="t in v305 , c in wild-type" + /replace="t" + variation 35622 + /note="t in v305 , g in wild-type" + /replace="t" + misc_signal complement(35635..35651) + /note="operator-l3 (first base on comp strand)" + gene complement(35798..38343) + /locus_tag="lambdap50" + /db_xref="GeneID:2703537" + mRNA complement(35798..38343) + /locus_tag="lambdap50" + /product="mRNA-pre (via timm terminator)" + /db_xref="GeneID:2703537" + gene complement(35798..37940) + /locus_tag="lambdap51" + /db_xref="GeneID:2703538" + mRNA complement(35798..37940) + /locus_tag="lambdap51" + /product="mRNA-prm (via timm terminator)" + /db_xref="GeneID:2703538" + gene complement(35798..36256) + /locus_tag="lambdap52" + /db_xref="GeneID:2703465" + mRNA complement(35798..36256) + /locus_tag="lambdap52" + /product="mRNA-plit (via timm terminator)" + /db_xref="GeneID:2703465" + gene complement(35825..36259) + /gene="rexb" + /locus_tag="lambdap53" + /db_xref="GeneID:2703493" + CDS complement(35825..36259) + /gene="rexb" + /locus_tag="lambdap53" + /codon_start=1 + /transl_table=11 + /product="exclusion protein" + /protein_id="NP_040626.1" + /db_xref="GI:9626290" + /db_xref="GeneID:2703493" + /translation="MRNRIMPGVYIVIIPYVIVSICYLLFRHYIPGVSFSAHRDGLGA + TLSSYAGTMIAILIAALTFLIGSRTRRLAKIREYGYMTSVVIVYALSFVELGALFFCG + LLLLSSISGYMIPTIAIGIASASFIHICILVFQLYNLTREQE" + variation 35940 + /gene="rexb" + /locus_tag="lambdap53" + /note="a in rex209 , g in wild-type" + /replace="a" + variation 35947 + /gene="rexb" + /locus_tag="lambdap53" + /note="a in rex111 , g in wild-type" + /replace="a" + gene complement(36275..37114) + /gene="rexa" + /locus_tag="lambdap87" + /db_xref="GeneID:3827058" + CDS complement(36275..37114) + /gene="rexa" + /locus_tag="lambdap87" + /codon_start=1 + /transl_table=11 + /product="exclusion protein" + /protein_id="NP_040627.1" + /db_xref="GI:9626291" + /db_xref="GeneID:3827058" + /translation="MKNGFYATYRSKNKGKDKRSINLSVFLNSLLADNHHLQVGSNYL + YIHKIDGKTFLFTKTNDKSLVQKINRSKASVEDIKNSLADDESLGFPSFLFVEGDTIG + FARTVFGPTTSDLTDFLIGKGMSLSSGERVQIEPLMRGTTKDDVMHMHFIGRTTVKVE + AKLPVFGDILKVLGATDIEGELFDSLDIVIKPKFKRDIKKVAKDIIFNPSPQFSDISL + RAKDEAGDILTEHYLSEKGHLSAPLNKVTNAEIAEEMAYCYARMKSDILECFKRQVGK + VKD" + gene complement(37227..37940) + /gene="cI" + /locus_tag="lambdap88" + /db_xref="GeneID:3827059" + CDS complement(37227..37940) + /gene="cI" + /locus_tag="lambdap88" + /codon_start=1 + /transl_table=11 + /product="repressor" + /protein_id="NP_040628.1" + /db_xref="GI:9626292" + /db_xref="GeneID:3827059" + /translation="MSTKKKPLTQEQLEDARRLKAIYEKKKNELGLSQESVADKMGMG + QSGVGALFNGINALNAYNAALLAKILKVSVEEFSPSIAREIYEMYEAVSMQPSLRSEY + EYPVFSHVQAGMFSPELRTFTKGDAERWVSTTKKASDSAFWLEVEGNSMTAPTGSKPS + FPDGMLILVDPEQAVEPGDFCIARLGGDEFTFKKLIRDSGQVFLQPLNPQYPMIPCNE + SCSVVGKVIASQWPEETFG" + variation 37287 + /gene="cI" + /locus_tag="lambdap88" + /note="a in cIam14, c in wild-type" + /replace="a" + variation 37308 + /gene="cI" + /locus_tag="lambdap88" + /note="c in cIam504, g in wild-type" + /replace="c" + variation 37313 + /gene="cI" + /locus_tag="lambdap88" + /note="a in cIam505, g in wild-type" + /replace="a" + variation 37589 + /gene="cI" + /locus_tag="lambdap88" + /note="t in ind1; c in wild-type" + /replace="t" + variation 37629 + /gene="cI" + /locus_tag="lambdap88" + /note="c in cIam499; g in wild-type" + /replace="c" + variation 37635 + /gene="cI" + /locus_tag="lambdap88" + /note="c in cIam212, a in wild-type" + /replace="c" + variation 37680 + /gene="cI" + /locus_tag="lambdap88" + /note="a in cIam34; c in wild-type" + /replace="a" + variation 37742 + /gene="cI" + /locus_tag="lambdap88" + /note="t in strain ci857s7([25]); c in wild-type" + /replace="t" + variation 37808 + /gene="cI" + /locus_tag="lambdap88" + /note="a in cIam282; g in wild-type" + /replace="a" + variation 37872 + /gene="cI" + /locus_tag="lambdap88" + /note="c in cIam302; a in wild-type" + /replace="c" + misc_signal 37951..37967 + /note="operator-r3" + variation 37954 + /locus_tag="lambdap50" + /note="t in prm-e37; c in wild-type" + /replace="t" + variation 37955 + /locus_tag="lambdap50" + /note="g in vc3;, a in wild-type" + /replace="g" + variation 37957 + /locus_tag="lambdap50" + /note="t in or3-r1; c in wild-type" + /replace="t" + variation 37958 + /locus_tag="lambdap50" + /note="a in or 3-r3 mutants; g in wild-type" + /replace="a" + variation 37965 + /locus_tag="lambdap50" + /note="g in or3-c12; a in wild-type" + /replace="g" + variation 37966 + /locus_tag="lambdap50" + /note="c in or3-c10; t in wild-type" + /replace="c" + variation 37971 + /locus_tag="lambdap50" + /note="g inp-rmup-1; a in wild-type" + /replace="g" + variation 37973 + /locus_tag="lambdap50" + /note="t in prm-m104; 116; u31 mutants, c in wild-type" + /replace="t" + misc_signal 37974..37990 + /note="operator-r2" + variation 37978 + /locus_tag="lambdap50" + /note="g in vc3; a in wild-type" + /replace="g" + variation 37978 + /locus_tag="lambdap50" + /note="t in prm-e104; a in wild-type" + /replace="t" + variation 37979 + /locus_tag="lambdap50" + /note="t in prm-e93; c in wild-type" + /replace="t" + variation 37985 + /locus_tag="lambdap50" + /note="t in vn; g in wild-type" + /replace="t" + variation 37988..37990 + /locus_tag="lambdap50" + /note="tg in mah4 mutant; ttg in wt" + /replace="tg" + variation 37989..37991 + /locus_tag="lambdap50" + /note="ta in mch9 mutant; tga in wt" + /replace="ta" + variation 37991 + /locus_tag="lambdap50" + /note="g in pr-x3; a in wild-type" + /replace="g" + misc_signal 37998..38014 + /note="operator-r1" + variation 38003 + /locus_tag="lambdap50" + /note="a in vs326; c in wild-type" + /replace="a" + variation 38007 + /locus_tag="lambdap50" + /note="a in vir3; c in wild-type" + /replace="a" + variation 38007 + /locus_tag="lambdap50" + /note="t in prm-uv8; c in wild-type" + /replace="t" + variation 38009 + /locus_tag="lambdap50" + /note="t in vc1; g in wild-type" + /replace="t" + gene 38023..40624 + /locus_tag="lambdap54" + /db_xref="GeneID:2703490" + mRNA 38023..40624 + /locus_tag="lambdap54" + /product="mRNA-pr (alt.; via tr2 terminator)" + /db_xref="GeneID:2703490" + gene 38023..38370 + /locus_tag="lambdap55" + /db_xref="GeneID:2703491" + mRNA 38023..38370 + /locus_tag="lambdap55" + /product="mRNA-pr (alt.; via tr1c terminator)" + /db_xref="GeneID:2703491" + gene 38023..38337 + /locus_tag="lambdap56" + /db_xref="GeneID:2703466" + mRNA 38023..38337 + /locus_tag="lambdap56" + /product="mRNA-pr (alt.; via tr1b terminator)" + /db_xref="GeneID:2703466" + gene 38023..38315 + /gene="cro" + /locus_tag="lambdap57" + /db_xref="GeneID:2703467" + mRNA 38023..38315 + /gene="cro" + /locus_tag="lambdap57" + /product="mRNA-pr (alt.; via tr1a terminator)" + /db_xref="GeneID:2703467" + gene 38023..38135 + /locus_tag="lambdap58" + /db_xref="GeneID:2703539" + mRNA 38023..38135 + /locus_tag="lambdap58" + /product="mRNA-pr (alt.; via tr0 terminator)" + /db_xref="GeneID:2703539" + CDS 38041..38241 + /gene="cro" + /locus_tag="lambdap57" + /codon_start=1 + /transl_table=11 + /product="antirepressor" + /protein_id="NP_040629.1" + /db_xref="GI:9626293" + /db_xref="GeneID:2703467" + /translation="MEQRITLKDYAMRFGQTKTAKDLGVYQSAINKAIHAGRKIFLTI + NADGSVYAEEVKPFPSNKKTTA" + misc_feature 38249..38266 + /gene="cro" + /locus_tag="lambdap57" + /note="rho utilization site A (rutA)" + misc_binding 38265..38281 + /gene="cro" + /locus_tag="lambdap57" + /note="N-utilization rightward; putative" + /bound_moiety="Nutr" + misc_feature 38282..38301 + /gene="cro" + /locus_tag="lambdap57" + /note="rho utilization site B (rutB)" + variation 38302 + /gene="cro" + /locus_tag="lambdap57" + /note="a in cin-1; g in wild-type" + /replace="a" + variation 38306 + /gene="cro" + /locus_tag="lambdap57" + /note="c in cnc1; t in wild-type" + /replace="c" + variation 38307 + /gene="cro" + /locus_tag="lambdap57" + /note="g in cnc8; a in wild-type" + /replace="g" + variation 38350 + /locus_tag="lambdap55" + /note="g in cy3048; a in wild-type" + /replace="g" + variation 38354 + /locus_tag="lambdap55" + /note="c in cy2001; t in wild-type" + /replace="c" + variation 38357 + /locus_tag="lambdap55" + /note="t in cy3019; c in wild-type" + /replace="t" + gene 38360..38653 + /gene="cII" + /locus_tag="lambdap59" + /db_xref="GeneID:2703494" + CDS 38360..38653 + /gene="cII" + /locus_tag="lambdap59" + /function="transcriptional activator" + /codon_start=1 + /transl_table=11 + /product="cII protein" + /protein_id="NP_040630.1" + /db_xref="GI:9626294" + /db_xref="GeneID:2703494" + /translation="MVRANKRNEALRIESALLNKIAMLGTEKTAEAVGVDKSQISRWK + RDWIPKFSMLLAVLEWGVVDDDMARLARQVAAILTNKKRPAATERSEQIQMEF" + variation 38364 + /gene="cII" + /locus_tag="lambdap59" + /note="g in can1; t in wild-type" + /replace="g" + variation 38370 + /gene="cII" + /locus_tag="lambdap59" + /note="t in cy3003; c in wild-type" + /replace="t" + variation 38371 + /gene="cII" + /locus_tag="lambdap59" + /note="t in cy42; a in wild-type" + /replace="t" + variation 38376 + /gene="cII" + /locus_tag="lambdap59" + /note="g in cy844; a in wild-type" + /replace="g" + variation 38379 + /gene="cII" + /locus_tag="lambdap59" + /note="a in cy3008; g in wild-type" + /replace="a" + variation 38380 + /gene="cII" + /locus_tag="lambdap59" + /note="t in cy3001; c in wild-type" + /replace="t" + variation 38430 + /gene="cII" + /locus_tag="lambdap59" + /note="c in cII2002; t in wild-type" + /replace="c" + misc_signal 38543..38557 + /gene="cII" + /locus_tag="lambdap59" + /note="ice(inceptor signal for DNA replication)" + gene complement(38599..38675) + /locus_tag="lambdap60" + /db_xref="GeneID:2703492" + mRNA complement(38599..38675) + /locus_tag="lambdap60" + /product="mRNA-oop transcription mRNA" + /db_xref="GeneID:2703492" + gene 38686..39585 + /gene="O" + /locus_tag="lambdap89" + /db_xref="GeneID:3827060" + CDS 38686..39585 + /gene="O" + /locus_tag="lambdap89" + /codon_start=1 + /transl_table=11 + /product="DNA replication protein" + /protein_id="NP_040631.1" + /db_xref="GI:9626295" + /db_xref="GeneID:3827060" + /translation="MTNTAKILNFGRGNFAGQERNVADLDDGYARLSNMLLEAYSGAD + LTKRQFKVLLAILRKTYGWNKPMDRITDSQLSEITKLPVKRCNEAKLELVRMNIIKQQ + GGMFGPNKNISEWCIPQNEGKSPKTRDKTSLKLGDCYPSKQGDTKDTITKEKRKDYSS + ENSGESSDQPENDLSVVKPDAAIQSGSKWGTAEDLTAAEWMFDMVKTIAPSARKPNFA + GWANDIRLMRERDGRNHRDMCVLFRWACQDNFWSGNVLSPAKLRDKWTQLEINRNKQQ + AGVTASKPKLDLTNTDWIYGVDL" + misc_binding 39034..39051 + /gene="O" + /locus_tag="lambdap89" + /bound_moiety="ori iteron 1(O" + misc_binding 39054..39071 + /gene="O" + /locus_tag="lambdap89" + /bound_moiety="ori iteron 2(O" + misc_binding 39078..39095 + /gene="O" + /locus_tag="lambdap89" + /bound_moiety="ori iteron 3(O" + misc_binding 39101..39118 + /gene="O" + /locus_tag="lambdap89" + /bound_moiety="ori iteron 4(O" + variation 39122 + /gene="O" + /locus_tag="lambdap89" + /note="a in ti-12; c in wild-type" + /replace="a" + misc_recomb 39157..39158 + /gene="O" + /locus_tag="lambdap89" + misc_recomb 39165..39166 + /gene="O" + /locus_tag="lambdap89" + variation 39268 + /gene="O" + /locus_tag="lambdap89" + /note="t in ric5b; c in wild-type" + /replace="t" + variation 39292 + /gene="O" + /locus_tag="lambdap89" + /note="a in ric5b; g in wild-type" + /replace="a" + gene 39582..40283 + /gene="P" + /locus_tag="lambdap61" + /db_xref="GeneID:2703495" + CDS 39582..40283 + /gene="P" + /locus_tag="lambdap61" + /codon_start=1 + /transl_table=11 + /product="DNA replication protein" + /protein_id="NP_040632.1" + /db_xref="GI:9626296" + /db_xref="GeneID:2703495" + /translation="MKNIAAQMVNFDREQMRRIANNMPEQYDEKPQVQQVAQIINGVF + SQLLATFPASLANRDQNEVNEIRRQWVLAFRENGITTMEQVNAGMRVARRQNRPFLPS + PGQFVAWCREEASVTAGLPNVSELVDMVYEYCRKRGLYPDAESYPWKSNAHYWLVTNL + YQNMRANALTDAELRRKAADELVHMTARINRGEAIPEPVKQLPVMGGRPLNRAQALAK + IAEIKAKFGLKGASV" + gene 40280..40570 + /gene="ren" + /locus_tag="lambdap62" + /db_xref="GeneID:2703496" + CDS 40280..40570 + /gene="ren" + /locus_tag="lambdap62" + /codon_start=1 + /transl_table=11 + /product="ren exclusion protein" + /protein_id="NP_040633.1" + /db_xref="GI:9626297" + /db_xref="GeneID:2703496" + /translation="MTGKEAIIHYLGTHNSFCAPDVAALTGATVTSINQAAAKMARAG + LLVIEGKVWRTVYYRFATREEREGKMSTNLVFKECRQSAAMKRVLAVYGVKR" + variation 40501..43307 + /note="Nin5 substitution" + gene 40644..41084 + /gene="NinB" + /locus_tag="lambdap63" + /db_xref="GeneID:2703497" + CDS 40644..41084 + /gene="NinB" + /locus_tag="lambdap63" + /codon_start=1 + /transl_table=11 + /product="NinB" + /protein_id="NP_040634.1" + /db_xref="GI:9626298" + /db_xref="GeneID:2703497" + /translation="MKKLTFEIRSPAHQQNAIHAVQQILPDPTKPIVVTIQERNRSLD + QNRKLWACLGDVSRQVEWHGRWLDAESWKCVFTAALKQQDVVPNLAGNGFVVIGQSTS + RMRVGEFAELLELIQAFGTERGVKWSDEARLALEWKARWGDRAA" + gene 41081..41953 + /gene="NinC" + /locus_tag="lambdap64" + /db_xref="GeneID:2703498" + CDS 41081..41953 + /gene="NinC" + /locus_tag="lambdap64" + /codon_start=1 + /transl_table=11 + /product="NinC protein" + /protein_id="NP_040635.1" + /db_xref="GI:9626299" + /db_xref="GeneID:2703498" + /translation="MINVVSFSGGRTSAYLLWLMEQKRRAGKDVHYVFMDTGCEHPMT + YRFVREVVKFWDIPLTVLQVDINPELGQPNGYTVWEPKDIQTRMPVLKPFIDMVKKYG + TPYVGGAFCTDRLKLVPFTKYCDDHFGRGNYTTWIGIRADEPKRLKPKPGIRYLAELS + DFEKEDILAWWKQQPFDLQIPEHLGNCIFCIKKSTQKIGLACKDEEGLQRVFNEVITG + SHVRDGHRETPKEIMYRGRMSLDGIAKMYSENDYQALYQDMVRAKRFDTGSCSESCEI + FGGQLDFDFGREAA" + gene 41950..42123 + /gene="NinD" + /locus_tag="lambdap65" + /db_xref="GeneID:2703499" + CDS 41950..42123 + /gene="NinD" + /locus_tag="lambdap65" + /codon_start=1 + /transl_table=11 + /product="NinD protein" + /protein_id="NP_040636.1" + /db_xref="GI:9626300" + /db_xref="GeneID:2703499" + /translation="MMRCYRCGECKEDNRFRPNQPYWNRWCLRCERTPTGVLPLPQEK + EDVWRDSDEVSPT" + gene 42090..42272 + /gene="NinE" + /locus_tag="lambdap66" + /db_xref="GeneID:2703500" + CDS 42090..42272 + /gene="NinE" + /locus_tag="lambdap66" + /codon_start=1 + /transl_table=11 + /product="NinE protein" + /protein_id="NP_040637.1" + /db_xref="GI:9626301" + /db_xref="GeneID:2703500" + /translation="MARQRRSITDIICENCKYLPTKRTRNKPKPIPKESDVKTFNYTA + HLWDIRWLRRRARKTR" + gene 42269..42439 + /gene="NinF" + /locus_tag="lambdap67" + /db_xref="GeneID:2703501" + CDS 42269..42439 + /gene="NinF" + /locus_tag="lambdap67" + /codon_start=1 + /transl_table=11 + /product="NinF protein" + /protein_id="NP_040638.1" + /db_xref="GI:9626302" + /db_xref="GeneID:2703501" + /translation="MIDQNRSYEQESVERALTCANCGQKLHVLEVHVCEHCCAELMSD + PNSSMHEEEDDG" + gene 42429..43043 + /gene="NinG" + /locus_tag="lambdap68" + /db_xref="GeneID:2703474" + CDS 42429..43043 + /gene="NinG" + /locus_tag="lambdap68" + /codon_start=1 + /transl_table=11 + /product="NinG protein" + /protein_id="NP_040639.1" + /db_xref="GI:9626303" + /db_xref="GeneID:2703474" + /translation="MMAKPARRRCKNDECREWFHPAFANQWWCSPECGTKIALERRSK + EREKAEKAAEKKRRREEQKQKDKLKIRKLALKPRSYWIKQAQQAVNAFIRERDRDLPC + ISCGTLTSAQWDAGHYRTTAAAPQLRFNERNIHKQCVVCNQHKSGNLVPYRVELISRI + GQEAVDEIESNHNRHRWTIEECKAIKAEYQQKLKDLRNSRSEAA" + gene 43040..43246 + /gene="NinH" + /locus_tag="lambdap69" + /db_xref="GeneID:2703475" + CDS 43040..43246 + /gene="NinH" + /locus_tag="lambdap69" + /codon_start=1 + /transl_table=11 + /product="NinH protein" + /protein_id="NP_040640.1" + /db_xref="GI:9626304" + /db_xref="GeneID:2703475" + /translation="MTFSVKTIPDMLVEAYGNQTEVARRLKCSRGTVRKYVDDKDGKM + HAIVNDVLMVHRGWSERDALLRKN" + unsure 43082 + /gene="NinH" + /locus_tag="lambdap69" + /note="g or a, cited in [(in) Hendrix,R.W., Roberts,J.W., + Stahl,F.W. andWeisberg,R.A. (Eds.);Lambda II: 4]" + /replace="a" + variation 43082 + /gene="NinH" + /locus_tag="lambdap69" + /note="a in strain cI857s7 ([25]); g in wild-type" + /replace="a" + gene 43224..43889 + /gene="NinI" + /locus_tag="lambdap70" + /db_xref="GeneID:2703476" + CDS 43224..43889 + /gene="NinI" + /locus_tag="lambdap70" + /function="serine/threonine phosphatase" + /codon_start=1 + /transl_table=11 + /product="NinI protein" + /protein_id="NP_040641.1" + /db_xref="GI:9626305" + /db_xref="GeneID:2703476" + /translation="MRYYEKIDGSKYRNIWVVGDLHGCYTNLMNKLDTIGFDNKKDLL + ISVGDLVDRGAENVECLELITFPWFRAVRGNHEQMMIDGLSERGNVNHWLLNGGGWFF + NLDYDKEILAKALAHKADELPLIIELVSKDKKYVICHADYPFDEYEFGKPVDHQQVIW + NRERISNSQNGIVKEIKGADTFIFGHTPAVKPLKFANQMYIDTGAVFCGNLTLIQVQG + EGA" + misc_recomb 43884..43885 + /gene="NinI" + /locus_tag="lambdap70" + gene 43886..44509 + /gene="Q" + /locus_tag="lambdap71" + /db_xref="GeneID:2703477" + CDS 43886..44509 + /gene="Q" + /locus_tag="lambdap71" + /codon_start=1 + /transl_table=11 + /product="late gene regulator" + /protein_id="NP_040642.1" + /db_xref="GI:9626306" + /db_xref="GeneID:2703477" + /translation="MRLESVAKFHSPKSPMMSDSPRATASDSLSGTDVMAAMGMAQSQ + AGFGMAAFCGKHELSQNDKQKAINYLMQFAHKVSGKYRGVAKLEGNTKAKVLQVLATF + AYADYCRSAATPGARCRDCHGTGRAVDIAKTELWGRVVEKECGRCKGVGYSRMPASAA + YRAVTMLIPNLTQPTWSRTVKPLYDALVVQCHKEESIADNILNAVTR" + gene 44587..44780 + /locus_tag="lambdap72" + /db_xref="GeneID:2703521" + mRNA 44587..44780 + /locus_tag="lambdap72" + /product="mRNA-pr' transcription (late genes) mRNA" + /db_xref="GeneID:2703521" + gene 44621..44815 + /gene="orf-64" + /locus_tag="lambdap73" + /db_xref="GeneID:2703478" + CDS 44621..44815 + /gene="orf-64" + /locus_tag="lambdap73" + /codon_start=1 + /transl_table=11 + /product="hypothetical protein" + /protein_id="NP_040643.1" + /db_xref="GI:9626307" + /db_xref="GeneID:2703478" + /translation="MKRGGAYYRFRLVGHFDVSSGTPTIAGREVCKMQSRNSSQVIVR + ACITVSGFFISAQQVRALSR" + gene 45186..45509 + /gene="S" + /locus_tag="lambdap74" + /db_xref="GeneID:2703479" + CDS 45186..45509 + /gene="S" + /locus_tag="lambdap74" + /codon_start=1 + /transl_table=11 + /product="anti-holin" + /protein_id="NP_040644.1" + /db_xref="GI:9626308" + /db_xref="GeneID:2703479" + /translation="MKMPEKHDLLAAILAAKEQGIGAILAFAMAYLRGRYNGGAFTKT + VIDATMCAIIAWFIRDLLDFAGLSSNLAYITSVFIGYIGTDSIGSLIKRFAAKKAGVE + DGRNQ" + gene 45192..45509 + /gene="S'" + /locus_tag="lambdap92" + /db_xref="GeneID:5740919" + CDS 45192..45509 + /gene="S'" + /locus_tag="lambdap92" + /codon_start=1 + /transl_table=11 + /product="holin" + /protein_id="YP_001551775.1" + /db_xref="GI:160380505" + /db_xref="GeneID:5740919" + /translation="MPEKHDLLAAILAAKEQGIGAILAFAMAYLRGRYNGGAFTKTVI + DATMCAIIAWFIRDLLDFAGLSSNLAYITSVFIGYIGTDSIGSLIKRFAAKKAGVEDG + RNQ" + variation 45352 + /gene="S'" + /locus_tag="lambdap92" + /note="a in sam7; g in wild-type" + /replace="a" + variation 45352 + /gene="S'" + /locus_tag="lambdap92" + /note="a in strain cI857s7 ([25]); g in wild-type" + /replace="a" + gene 45493..45969 + /gene="R" + /locus_tag="lambdap75" + /db_xref="GeneID:2703480" + CDS 45493..45969 + /gene="R" + /locus_tag="lambdap75" + /codon_start=1 + /transl_table=11 + /product="endolysin" + /protein_id="NP_040645.1" + /db_xref="GI:9626309" + /db_xref="GeneID:2703480" + /translation="MVEINNQRKAFLDMLAWSEGTDNGRQKTRNHGYDVIVGGELFTD + YSDHPRKLVTLNPKLKSTGAGRYQLLSRWWDAYRKQLGLKDFSPKSQDAVALQQIKER + GALPMIDRGDIRQAIDRCSNIWASLPGAGYGQFEHKADSLIAKFKEAGGTVREIDV" + gene 45966..46185 + /gene="Rz" + /locus_tag="lambdap76" + /db_xref="GeneID:2703481" + CDS 45966..46185 + /gene="Rz" + /locus_tag="lambdap76" + /codon_start=1 + /transl_table=11 + /product="cell lysis protein" + /protein_id="NP_040646.1" + /db_xref="GI:9626310" + /db_xref="GeneID:2703481" + /translation="MSRVTAIISALVICIIVCLSWAVNHYRDNAITYKAQRDKNAREL + KLANAAITDMQMRQRDVAALDAKYTKELADAKAENDALRDDVAAGRRRLHIKAVCQSV + REATTASGVDNAASPRLADTAERDYFTLRERLITMQKQLEGTQKYINEQCR" + gene 46186..46368 + /gene="Rz1" + /locus_tag="lambdap91" + /db_xref="GeneID:5739319" + CDS 46186..46368 + /gene="Rz1" + /locus_tag="lambdap91" + /codon_start=1 + /transl_table=11 + /product="Rz1 protein" + /protein_id="YP_001551744.1" + /db_xref="GI:160338810" + /db_xref="GeneID:5739319" + /translation="MLKLKMMLCVMMLPLVVVGCTSKQSVSQCVKPPPPPAWIMQPPP + DWQTPLNGIISPSERG" + gene complement(46459..46752) + /gene="bor" + /locus_tag="lambdap77" + /db_xref="GeneID:2703532" + CDS complement(46459..46752) + /gene="bor" + /locus_tag="lambdap77" + /function="confers serum resistance upon the host" + /note="Predicted by GeneMark" + /codon_start=1 + /transl_table=11 + /product="Bor protein precursor" + /protein_id="NP_597780.1" + /db_xref="GI:19263395" + /db_xref="GeneID:2703532" + /translation="MKKMLLATALALLITGCAQQTFTVQNKPAAVAPKETITHHFFVS + GIGQKKTVDAAKICGGAENVVKTETQQTFVNGLLGFITLGIYTPLEARVYCSQ" + gene complement(47142 ..47575) + /locus_tag="lambdap78" + /db_xref="GeneID:2703533" + CDS complement(47142..47575) + /locus_tag="lambdap78" + /note="Predicted by GeneMark" + /codon_start=1 + /transl_table=11 + /product="putative envelope protein" + /protein_id="NP_597781.1" + /db_xref="GI:19263396" + /db_xref="GeneID:2703533" + /translation="MKTFLIFDINHQQNTRRSDQIETIKTIMQTTRPRITWKVLPMAQ + VAIFKEIFDQVRKDLDCELFYSELKRHNVSHYIYYLATDNIHIVLENDNTVLIKGLKK + VVNVKFSRNTHLIETSYDRLKSREITFQQYRENLAKAGVFRWVTNIHEHKRYYYTFDN + SLLFTESIQNTTQIFPR" + gene 47738..47944 + /locus_tag="lambdap79" + /db_xref="GeneID:2703534" + CDS 47738..47944 + /locus_tag="lambdap79" + /note="Predicted by GeneMark" + /codon_start=1 + /transl_table=11 + /product="hypothetical protein" + /protein_id="NP_597782.1" + /db_xref="GI:19263397" + /db_xref="GeneID:2703534" + /translation="MNKEQSADDPSVDLIRVKNMLNSTISMSYPDVVIACIEHKVSLE + AFRAIEAALVKHDNNMKDYSLVVD" +ORIGIN + 1 gggcggcgac ctcgcgggtt ttcgctattt atgaaaattt tccggtttaa ggcgtttccg + 61 ttcttcttcg tcataactta atgtttttat ttaaaatacc ctctgaaaag aaaggaaacg + 121 acaggtgctg aaagcgaggc tttttggcct ctgtcgtttc ctttctctgt ttttgtccgt + 181 ggaatgaaca atggaagtca acaaaaagca gctggctgac attttcggtg cgagtatccg + 241 taccattcag aactggcagg aacagggaat gcccgttctg cgaggcggtg gcaagggtaa + 301 tgaggtgctt tatgactctg ccgccgtcat aaaatggtat gccgaaaggg atgctgaaat + 361 tgagaacgaa aagctgcgcc gggaggttga agaactgcgg caggccagcg aggcagatct + 421 ccagccagga actattgagt acgaacgcca tcgacttacg cgtgcgcagg ccgacgcaca + 481 ggaactgaag aatgccagag actccgctga agtggtggaa accgcattct gtactttcgt + 541 gctgtcgcgg atcgcaggtg aaattgccag tattctcgac gggctccccc tgtcggtgca + 601 gcggcgtttt ccggaactgg aaaaccgaca tgttgatttc ctgaaacggg atatcatcaa + 661 agccatgaac aaagcagccg cgctggatga actgataccg gggttgctga gtgaatatat + 721 cgaacagtca ggttaacagg ctgcggcatt ttgtccgcgc cgggcttcgc tcactgttca + 781 ggccggagcc acagaccgcc gttgaatggg cggatgctaa ttactatctc ccgaaagaat + 841 ccgcatacca ggaagggcgc tgggaaacac tgccctttca gcgggccatc atgaatgcga + 901 tgggcagcga ctacatccgt gaggtgaatg tggtgaagtc tgcccgtgtc ggttattcca + 961 aaatgctgct gggtgtttat gcctacttta tagagcataa gcagcgcaac acccttatct + 1021 ggttgccgac ggatggtgat gccgagaact ttatgaaaac ccacgttgag ccgactattc + 1081 gtgatattcc gtcgctgctg gcgctggccc cgtggtatgg caaaaagcac cgggataaca + 1141 cgctcaccat gaagcgtttc actaatgggc gtggcttctg gtgcctgggc ggtaaagcgg + 1201 caaaaaacta ccgtgaaaag tcggtggatg tggcgggtta tgatgaactt gctgcttttg + 1261 atgatgatat tgaacaggaa ggctctccga cgttcctggg tgacaagcgt attgaaggct + 1321 cggtctggcc aaagtccatc cgtggctcca cgccaaaagt gagaggcacc tgtcagattg + 1381 agcgtgcagc cagtgaatcc ccgcatttta tgcgttttca tgttgcctgc ccgcattgcg + 1441 gggaggagca gtatcttaaa tttggcgaca aagagacgcc gtttggcctc aaatggacgc + 1501 cggatgaccc ctccagcgtg ttttatctct gcgagcataa tgcctgcgtc atccgccagc + 1561 aggagctgga ctttactgat gcccgttata tctgcgaaaa gaccgggatc tggacccgtg + 1621 atggcattct ctggttttcg tcatccggtg aagagattga gccacctgac agtgtgacct + 1681 ttcacatctg gacagcgtac agcccgttca ccacctgggt gcagattgtc aaagactgga + 1741 tgaaaacgaa aggggatacg ggaaaacgta aaaccttcgt aaacaccacg ctcggtgaga + 1801 cgtgggaggc gaaaattggc gaacgtccgg atgctgaagt gatggcagag cggaaagagc + 1861 attattcagc gcccgttcct gaccgtgtgg cttacctgac cgccggtatc gactcccagc + 1921 tggaccgcta cgaaatgcgc gtatggggat gggggccggg tgaggaaagc tggctgattg + 1981 accggcagat tattatgggc cgccacgacg atgaacagac gctgctgcgt gtggatgagg + 2041 ccatcaataa aacctatacc cgccggaatg gtgcagaaat gtcgatatcc cgtatctgct + 2101 gggatactgg cgggattgac ccgaccattg tgtatgaacg ctcgaaaaaa catgggctgt + 2161 tccgggtgat ccccattaaa ggggcatccg tctacggaaa gccggtggcc agcatgccac + 2221 gtaagcgaaa caaaaacggg gtttacctta ccgaaatcgg tacggatacc gcgaaagagc + 2281 agatttataa ccgcttcaca ctgacgccgg aaggggatga accgcttccc ggtgccgttc + 2341 acttcccgaa taacccggat atttttgatc tgaccgaagc gcagcagctg actgctgaag + 2401 agcaggtcga aaaatgggtg gatggcagga aaaaaatact gtgggacagc aaaaagcgac + 2461 gcaatgaggc actcgactgc ttcgtttatg cgctggcggc gctgcgcatc agtatttccc + 2521 gctggcagct ggatctcagt gcgctgctgg cgagcctgca ggaagaggat ggtgcagcaa + 2581 ccaacaagaa aacactggca gattacgccc gtgccttatc cggagaggat gaatgacgcg + 2641 acaggaagaa cttgccgctg cccgtgcggc actgcatgac ctgatgacag gtaaacgggt + 2701 ggcaacagta cagaaagacg gacgaagggt ggagtttacg gccacttccg tgtctgacct + 2761 gaaaaaatat attgcagagc tggaagtgca gaccggcatg acacagcgac gcaggggacc + 2821 tgcaggattt tatgtatgaa aacgcccacc attcccaccc ttctggggcc ggacggcatg + 2881 acatcgctgc gcgaatatgc cggttatcac ggcggtggca gcggatttgg agggcagttg + 2941 cggtcgtgga acccaccgag tgaaagtgtg gatgcagccc tgttgcccaa ctttacccgt + 3001 ggcaatgccc gcgcagacga tctggtacgc aataacggct atgccgccaa cgccatccag + 3061 ctgcatcagg atcatatcgt cgggtctttt ttccggctca gtcatcgccc aagctggcgc + 3121 tatctgggca tcggggagga agaagcccgt gccttttccc gcgaggttga agcggcatgg + 3181 aaagagtttg ccgaggatga ctgctgctgc attgacgttg agcgaaaacg cacgtttacc + 3241 atgatgattc gggaaggtgt ggccatgcac gcctttaacg gtgaactgtt cgttcaggcc + 3301 acctgggata ccagttcgtc gcggcttttc cggacacagt tccggatggt cagcccgaag + 3361 cgcatcagca acccgaacaa taccggcgac agccggaact gccgtgccgg tgtgcagatt + 3421 aatgacagcg gtgcggcgct gggatattac gtcagcgagg acgggtatcc tggctggatg + 3481 ccgcagaaat ggacatggat accccgtgag ttacccggcg ggcgcgcctc gttcattcac + 3541 gtttttgaac ccgtggagga cgggcagact cgcggtgcaa atgtgtttta cagcgtgatg + 3601 gagcagatga agatgctcga cacgctgcag aacacgcagc tgcagagcgc cattgtgaag + 3661 gcgatgtatg ccgccaccat tgagagtgag ctggatacgc agtcagcgat ggattttatt + 3721 ctgggcgcga acagtcagga gcagcgggaa aggctgaccg gctggattgg tgaaattgcc + 3781 gcgtattacg ccgcagcgcc ggtccggctg ggaggcgcaa aagtaccgca cctgatgccg + 3841 ggtgactcac tgaacctgca gacggctcag gatacggata acggctactc cgtgtttgag + 3901 cagtcactgc tgcggtatat cgctgccggg ctgggtgtct cgtatgagca gctttcccgg + 3961 aattacgccc agatgagcta ctccacggca cgggccagtg cgaacgagtc gtgggcgtac + 4021 tttatggggc ggcgaaaatt cgtcgcatcc cgtcaggcga gccagatgtt tctgtgctgg + 4081 ctggaagagg ccatcgttcg ccgcgtggtg acgttacctt caaaagcgcg cttcagtttt + 4141 caggaagccc gcagtgcctg ggggaactgc gactggatag gctccggtcg tatggccatc + 4201 gatggtctga aagaagttca ggaagcggtg atgctgatag aagccggact gagtacctac + 4261 gagaaagagt gcgcaaaacg cggtgacgac tatcaggaaa tttttgccca gcaggtccgt + 4321 gaaacgatgg agcgccgtgc agccggtctt aaaccgcccg cctgggcggc tgcagcattt + 4381 gaatccgggc tgcgacaatc aacagaggag gagaagagtg acagcagagc tgcgtaatct + 4441 cccgcatatt gccagcatgg cctttaatga gccgctgatg cttgaacccg cctatgcgcg + 4501 ggttttcttt tgtgcgcttg caggccagct tgggatcagc agcctgacgg atgcggtgtc + 4561 cggcgacagc ctgactgccc aggaggcact cgcgacgctg gcattatccg gtgatgatga + 4621 cggaccacga caggcccgca gttatcaggt catgaacggc atcgccgtgc tgccggtgtc + 4681 cggcacgctg gtcagccgga cgcgggcgct gcagccgtac tcggggatga ccggttacaa + 4741 cggcattatc gcccgtctgc aacaggctgc cagcgatccg atggtggacg gcattctgct + 4801 cgatatggac acgcccggcg ggatggtggc gggggcattt gactgcgctg acatcatcgc + 4861 ccgtgtgcgt gacataaaac cggtatgggc gcttgccaac gacatgaact gcagtgcagg + 4921 tcagttgctt gccagtgccg cctcccggcg tctggtcacg cagaccgccc ggacaggctc + 4981 catcggcgtc atgatggctc acagtaatta cggtgctgcg ctggagaaac agggtgtgga + 5041 aatcacgctg atttacagcg gcagccataa ggtggatggc aacccctaca gccatcttcc + 5101 ggatgacgtc cgggagacac tgcagtcccg gatggacgca acccgccaga tgtttgcgca + 5161 gaaggtgtcg gcatataccg gcctgtccgt gcaggttgtg ctggataccg aggctgcagt + 5221 gtacagcggt caggaggcca ttgatgccgg actggctgat gaacttgtta acagcaccga + 5281 tgcgatcacc gtcatgcgtg atgcactgga tgcacgtaaa tcccgtctct caggagggcg + 5341 aatgaccaaa gagactcaat caacaactgt ttcagccact gcttcgcagg ctgacgttac + 5401 tgacgtggtg ccagcgacgg agggcgagaa cgccagcgcg gcgcagccgg acgtgaacgc + 5461 gcagatcacc gcagcggttg cggcagaaaa cagccgcatt atggggatcc tcaactgtga + 5521 ggaggctcac ggacgcgaag aacaggcacg cgtgctggca gaaacccccg gtatgaccgt + 5581 gaaaacggcc cgccgcattc tggccgcagc accacagagt gcacaggcgc gcagtgacac + 5641 tgcgctggat cgtctgatgc agggggcacc ggcaccgctg gctgcaggta acccggcatc + 5701 tgatgccgtt aacgatttgc tgaacacacc agtgtaaggg atgtttatga cgagcaaaga + 5761 aacctttacc cattaccagc cgcagggcaa cagtgacccg gctcataccg caaccgcgcc + 5821 cggcggattg agtgcgaaag cgcctgcaat gaccccgctg atgctggaca cctccagccg + 5881 taagctggtt gcgtgggatg gcaccaccga cggtgctgcc gttggcattc ttgcggttgc + 5941 tgctgaccag accagcacca cgctgacgtt ctacaagtcc ggcacgttcc gttatgagga + 6001 tgtgctctgg ccggaggctg ccagcgacga gacgaaaaaa cggaccgcgt ttgccggaac + 6061 ggcaatcagc atcgtttaac tttacccttc atcactaaag gccgcctgtg cggctttttt + 6121 tacgggattt ttttatgtcg atgtacacaa ccgcccaact gctggcggca aatgagcaga + 6181 aatttaagtt tgatccgctg tttctgcgtc tctttttccg tgagagctat cccttcacca + 6241 cggagaaagt ctatctctca caaattccgg gactggtaaa catggcgctg tacgtttcgc + 6301 cgattgtttc cggtgaggtt atccgttccc gtggcggctc cacctctgaa tttacgccgg + 6361 gatatgtcaa gccgaagcat gaagtgaatc cgcagatgac cctgcgtcgc ctgccggatg + 6421 aagatccgca gaatctggcg gacccggctt accgccgccg tcgcatcatc atgcagaaca + 6481 tgcgtgacga agagctggcc attgctcagg tcgaagagat gcaggcagtt tctgccgtgc + 6541 ttaagggcaa atacaccatg accggtgaag ccttcgatcc ggttgaggtg gatatgggcc + 6601 gcagtgagga gaataacatc acgcagtccg gcggcacgga gtggagcaag cgtgacaagt + 6661 ccacgtatga cccgaccgac gatatcgaag cctacgcgct gaacgccagc ggtgtggtga + 6721 atatcatcgt gttcgatccg aaaggctggg cgctgttccg ttccttcaaa gccgtcaagg + 6781 agaagctgga tacccgtcgt ggctctaatt ccgagctgga gacagcggtg aaagacctgg + 6841 gcaaagcggt gtcctataag gggatgtatg gcgatgtggc catcgtcgtg tattccggac + 6901 agtacgtgga aaacggcgtc aaaaagaact tcctgccgga caacacgatg gtgctgggga + 6961 acactcaggc acgcggtctg cgcacctatg gctgcattca ggatgcggac gcacagcgcg + 7021 aaggcattaa cgcctctgcc cgttacccga aaaactgggt gaccaccggc gatccggcgc + 7081 gtgagttcac catgattcag tcagcaccgc tgatgctgct ggctgaccct gatgagttcg + 7141 tgtccgtaca actggcgtaa tcatggccct tcggggccat tgtttctctg tggaggagtc + 7201 catgacgaaa gatgaactga ttgcccgtct ccgctcgctg ggtgaacaac tgaaccgtga + 7261 tgtcagcctg acggggacga aagaagaact ggcgctccgt gtggcagagc tgaaagagga + 7321 gcttgatgac acggatgaaa ctgccggtca ggacacccct ctcagccggg aaaatgtgct + 7381 gaccggacat gaaaatgagg tgggatcagc gcagccggat accgtgattc tggatacgtc + 7441 tgaactggtc acggtcgtgg cactggtgaa gctgcatact gatgcacttc acgccacgcg + 7501 ggatgaacct gtggcatttg tgctgccggg aacggcgttt cgtgtctctg ccggtgtggc + 7561 agccgaaatg acagagcgcg gcctggccag aatgcaataa cgggaggcgc tgtggctgat + 7621 ttcgataacc tgttcgatgc tgccattgcc cgcgccgatg aaacgatacg cgggtacatg + 7681 ggaacgtcag ccaccattac atccggtgag cagtcaggtg cggtgatacg tggtgttttt + 7741 gatgaccctg aaaatatcag ctatgccgga cagggcgtgc gcgttgaagg ctccagcccg + 7801 tccctgtttg tccggactga tgaggtgcgg cagctgcggc gtggagacac gctgaccatc + 7861 ggtgaggaaa atttctgggt agatcgggtt tcgccggatg atggcggaag ttgtcatctc + 7921 tggcttggac ggggcgtacc gcctgccgtt aaccgtcgcc gctgaaaggg ggatgtatgg + 7981 ccataaaagg tcttgagcag gccgttgaaa acctcagccg tatcagcaaa acggcggtgc + 8041 ctggtgccgc cgcaatggcc attaaccgcg ttgcttcatc cgcgatatcg cagtcggcgt + 8101 cacaggttgc ccgtgagaca aaggtacgcc ggaaactggt aaaggaaagg gccaggctga + 8161 aaagggccac ggtcaaaaat ccgcaggcca gaatcaaagt taaccggggg gatttgcccg + 8221 taatcaagct gggtaatgcg cgggttgtcc tttcgcgccg caggcgtcgt aaaaaggggc + 8281 agcgttcatc cctgaaaggt ggcggcagcg tgcttgtggt gggtaaccgt cgtattcccg + 8341 gcgcgtttat tcagcaactg aaaaatggcc ggtggcatgt catgcagcgt gtggctggga + 8401 aaaaccgtta ccccattgat gtggtgaaaa tcccgatggc ggtgccgctg accacggcgt + 8461 ttaaacaaaa tattgagcgg atacggcgtg aacgtcttcc gaaagagctg ggctatgcgc + 8521 tgcagcatca actgaggatg gtaataaagc gatgaaacat actgaactcc gtgcagccgt + 8581 actggatgca ctggagaagc atgacaccgg ggcgacgttt tttgatggtc gccccgctgt + 8641 ttttgatgag gcggattttc cggcagttgc cgtttatctc accggcgctg aatacacggg + 8701 cgaagagctg gacagcgata cctggcaggc ggagctgcat atcgaagttt tcctgcctgc + 8761 tcaggtgccg gattcagagc tggatgcgtg gatggagtcc cggatttatc cggtgatgag + 8821 cgatatcccg gcactgtcag atttgatcac cagtatggtg gccagcggct atgactaccg + 8881 gcgcgacgat gatgcgggct tgtggagttc agccgatctg acttatgtca ttacctatga + 8941 aatgtgagga cgctatgcct gtaccaaatc ctacaatgcc ggtgaaaggt gccgggacca + 9001 ccctgtgggt ttataagggg agcggtgacc cttacgcgaa tccgctttca gacgttgact + 9061 ggtcgcgtct ggcaaaagtt aaagacctga cgcccggcga actgaccgct gagtcctatg + 9121 acgacagcta tctcgatgat gaagatgcag actggactgc gaccgggcag gggcagaaat + 9181 ctgccggaga taccagcttc acgctggcgt ggatgcccgg agagcagggg cagcaggcgc + 9241 tgctggcgtg gtttaatgaa ggcgataccc gtgcctataa aatccgcttc ccgaacggca + 9301 cggtcgatgt gttccgtggc tgggtcagca gtatcggtaa ggcggtgacg gcgaaggaag + 9361 tgatcacccg cacggtgaaa gtcaccaatg tgggacgtcc gtcgatggca gaagatcgca + 9421 gcacggtaac agcggcaacc ggcatgaccg tgacgcctgc cagcacctcg gtggtgaaag + 9481 ggcagagcac cacgctgacc gtggccttcc agccggaggg cgtaaccgac aagagctttc + 9541 gtgcggtgtc tgcggataaa acaaaagcca ccgtgtcggt cagtggtatg accatcaccg + 9601 tgaacggcgt tgctgcaggc aaggtcaaca ttccggttgt atccggtaat ggtgagtttg + 9661 ctgcggttgc agaaattacc gtcaccgcca gttaatccgg agagtcagcg atgttcctga + 9721 aaaccgaatc atttgaacat aacggtgtga ccgtcacgct ttctgaactg tcagccctgc + 9781 agcgcattga gcatctcgcc ctgatgaaac ggcaggcaga acaggcggag tcagacagca + 9841 accggaagtt tactgtggaa gacgccatca gaaccggcgc gtttctggtg gcgatgtccc + 9901 tgtggcataa ccatccgcag aagacgcaga tgccgtccat gaatgaagcc gttaaacaga + 9961 ttgagcagga agtgcttacc acctggccca cggaggcaat ttctcatgct gaaaacgtgg + 10021 tgtaccggct gtctggtatg tatgagtttg tggtgaataa tgcccctgaa cagacagagg + 10081 acgccgggcc cgcagagcct gtttctgcgg gaaagtgttc gacggtgagc tgagttttgc + 10141 cctgaaactg gcgcgtgaga tggggcgacc cgactggcgt gccatgcttg ccgggatgtc + 10201 atccacggag tatgccgact ggcaccgctt ttacagtacc cattattttc atgatgttct + 10261 gctggatatg cacttttccg ggctgacgta caccgtgctc agcctgtttt tcagcgatcc + 10321 ggatatgcat ccgctggatt tcagtctgct gaaccggcgc gaggctgacg aagagcctga + 10381 agatgatgtg ctgatgcaga aagcggcagg gcttgccgga ggtgtccgct ttggcccgga + 10441 cgggaatgaa gttatccccg cttccccgga tgtggcggac atgacggagg atgacgtaat + 10501 gctgatgaca gtatcagaag ggatcgcagg aggagtccgg tatggctgaa ccggtaggcg + 10561 atctggtcgt tgatttgagt ctggatgcgg ccagatttga cgagcagatg gccagagtca + 10621 ggcgtcattt ttctggtacg gaaagtgatg cgaaaaaaac agcggcagtc gttgaacagt + 10681 cgctgagccg acaggcgctg gctgcacaga aagcggggat ttccgtcggg cagtataaag + 10741 ccgccatgcg tatgctgcct gcacagttca ccgacgtggc cacgcagctt gcaggcgggc + 10801 aaagtccgtg gctgatcctg ctgcaacagg gggggcaggt gaaggactcc ttcggcggga + 10861 tgatccccat gttcaggggg cttgccggtg cgatcaccct gccgatggtg ggggccacct + 10921 cgctggcggt ggcgaccggt gcgctggcgt atgcctggta tcagggcaac tcaaccctgt + 10981 ccgatttcaa caaaacgctg gtcctttccg gcaatcaggc gggactgacg gcagatcgta + 11041 tgctggtcct gtccagagcc gggcaggcgg cagggctgac gtttaaccag accagcgagt + 11101 cactcagcgc actggttaag gcgggggtaa gcggtgaggc tcagattgcg tccatcagcc + 11161 agagtgtggc gcgtttctcc tctgcatccg gcgtggaggt ggacaaggtc gctgaagcct + 11221 tcgggaagct gaccacagac ccgacgtcgg ggctgacggc gatggctcgc cagttccata + 11281 acgtgtcggc ggagcagatt gcgtatgttg ctcagttgca gcgttccggc gatgaagccg + 11341 gggcattgca ggcggcgaac gaggccgcaa cgaaagggtt tgatgaccag acccgccgcc + 11401 tgaaagagaa catgggcacg ctggagacct gggcagacag gactgcgcgg gcattcaaat + 11461 ccatgtggga tgcggtgctg gatattggtc gtcctgatac cgcgcaggag atgctgatta + 11521 aggcagaggc tgcgtataag aaagcagacg acatctggaa tctgcgcaag gatgattatt + 11581 ttgttaacga tgaagcgcgg gcgcgttact gggatgatcg tgaaaaggcc cgtcttgcgc + 11641 ttgaagccgc ccgaaagaag gctgagcagc agactcaaca ggacaaaaat gcgcagcagc + 11701 agagcgatac cgaagcgtca cggctgaaat ataccgaaga ggcgcagaag gcttacgaac + 11761 ggctgcagac gccgctggag aaatataccg cccgtcagga agaactgaac aaggcactga + 11821 aagacgggaa aatcctgcag gcggattaca acacgctgat ggcggcggcg aaaaaggatt + 11881 atgaagcgac gctgaaaaag ccgaaacagt ccagcgtgaa ggtgtctgcg ggcgatcgtc + 11941 aggaagacag tgctcatgct gccctgctga cgcttcaggc agaactccgg acgctggaga + 12001 agcatgccgg agcaaatgag aaaatcagcc agcagcgccg ggatttgtgg aaggcggaga + 12061 gtcagttcgc ggtactggag gaggcggcgc aacgtcgcca gctgtctgca caggagaaat + 12121 ccctgctggc gcataaagat gagacgctgg agtacaaacg ccagctggct gcacttggcg + 12181 acaaggttac gtatcaggag cgcctgaacg cgctggcgca gcaggcggat aaattcgcac + 12241 agcagcaacg ggcaaaacgg gccgccattg atgcgaaaag ccgggggctg actgaccggc + 12301 aggcagaacg ggaagccacg gaacagcgcc tgaaggaaca gtatggcgat aatccgctgg + 12361 cgctgaataa cgtcatgtca gagcagaaaa agacctgggc ggctgaagac cagcttcgcg + 12421 ggaactggat ggcaggcctg aagtccggct ggagtgagtg ggaagagagc gccacggaca + 12481 gtatgtcgca ggtaaaaagt gcagccacgc agacctttga tggtattgca cagaatatgg + 12541 cggcgatgct gaccggcagt gagcagaact ggcgcagctt cacccgttcc gtgctgtcca + 12601 tgatgacaga aattctgctt aagcaggcaa tggtggggat tgtcgggagt atcggcagcg + 12661 ccattggcgg ggctgttggt ggcggcgcat ccgcgtcagg cggtacagcc attcaggccg + 12721 ctgcggcgaa attccatttt gcaaccggag gatttacggg aaccggcggc aaatatgagc + 12781 cagcggggat tgttcaccgt ggtgagtttg tcttcacgaa ggaggcaacc agccggattg + 12841 gcgtggggaa tctttaccgg ctgatgcgcg gctatgccac cggcggttat gtcggtacac + 12901 cgggcagcat ggcagacagc cggtcgcagg cgtccgggac gtttgagcag aataaccatg + 12961 tggtgattaa caacgacggc acgaacgggc agataggtcc ggctgctctg aaggcggtgt + 13021 atgacatggc ccgcaagggt gcccgtgatg aaattcagac acagatgcgt gatggtggcc + 13081 tgttctccgg aggtggacga tgaagacctt ccgctggaaa gtgaaacccg gtatggatgt + 13141 ggcttcggtc ccttctgtaa gaaaggtgcg ctttggtgat ggctattctc agcgagcgcc + 13201 tgccgggctg aatgccaacc tgaaaacgta cagcgtgacg ctttctgtcc cccgtgagga + 13261 ggccacggta ctggagtcgt ttctggaaga gcacgggggc tggaaatcct ttctgtggac + 13321 gccgccttat gagtggcggc agataaaggt gacctgcgca aaatggtcgt cgcgggtcag + 13381 tatgctgcgt gttgagttca gcgcagagtt tgaacaggtg gtgaactgat gcaggatatc + 13441 cggcaggaaa cactgaatga atgcacccgt gcggagcagt cggccagcgt ggtgctctgg + 13501 gaaatcgacc tgacagaggt cggtggagaa cgttattttt tctgtaatga gcagaacgaa + 13561 aaaggtgagc cggtcacctg gcaggggcga cagtatcagc cgtatcccat tcaggggagc + 13621 ggttttgaac tgaatggcaa aggcaccagt acgcgcccca cgctgacggt ttctaacctg + 13681 tacggtatgg tcaccgggat ggcggaagat atgcagagtc tggtcggcgg aacggtggtc + 13741 cggcgtaagg tttacgcccg ttttctggat gcggtgaact tcgtcaacgg aaacagttac + 13801 gccgatccgg agcaggaggt gatcagccgc tggcgcattg agcagtgcag cgaactgagc + 13861 gcggtgagtg cctcctttgt actgtccacg ccgacggaaa cggatggcgc tgtttttccg + 13921 ggacgtatca tgctggccaa cacctgcacc tggacctatc gcggtgacga gtgcggttat + 13981 agcggtccgg ctgtcgcgga tgaatatgac cagccaacgt ccgatatcac gaaggataaa + 14041 tgcagcaaat gcctgagcgg ttgtaagttc cgcaataacg tcggcaactt tggcggcttc + 14101 ctttccatta acaaactttc gcagtaaatc ccatgacaca gacagaatca gcgattctgg + 14161 cgcacgcccg gcgatgtgcg ccagcggagt cgtgcggctt cgtggtaagc acgccggagg + 14221 gggaaagata tttcccctgc gtgaatatct ccggtgagcc ggaggctatt tccgtatgtc + 14281 gccggaagac tggctgcagg cagaaatgca gggtgagatt gtggcgctgg tccacagcca + 14341 ccccggtggt ctgccctggc tgagtgaggc cgaccggcgg ctgcaggtgc agagtgattt + 14401 gccgtggtgg ctggtctgcc gggggacgat tcataagttc cgctgtgtgc cgcatctcac + 14461 cgggcggcgc tttgagcacg gtgtgacgga ctgttacaca ctgttccggg atgcttatca + 14521 tctggcgggg attgagatgc cggactttca tcgtgaggat gactggtggc gtaacggcca + 14581 gaatctctat ctggataatc tggaggcgac ggggctgtat caggtgccgt tgtcagcggc + 14641 acagccgggc gatgtgctgc tgtgctgttt tggttcatca gtgccgaatc acgccgcaat + 14701 ttactgcggc gacggcgagc tgctgcacca tattcctgaa caactgagca aacgagagag + 14761 gtacaccgac aaatggcagc gacgcacaca ctccctctgg cgtcaccggg catggcgcgc + 14821 atctgccttt acggggattt acaacgattt ggtcgccgca tcgaccttcg tgtgaaaacg + 14881 ggggctgaag ccatccgggc actggccaca cagctcccgg cgtttcgtca gaaactgagc + 14941 gacggctggt atcaggtacg gattgccggg cgggacgtca gcacgtccgg gttaacggcg + 15001 cagttacatg agactctgcc tgatggcgct gtaattcata ttgttcccag agtcgccggg + 15061 gccaagtcag gtggcgtatt ccagattgtc ctgggggctg ccgccattgc cggatcattc + 15121 tttaccgccg gagccaccct tgcagcatgg ggggcagcca ttggggccgg tggtatgacc + 15181 ggcatcctgt tttctctcgg tgccagtatg gtgctcggtg gtgtggcgca gatgctggca + 15241 ccgaaagcca gaactccccg tatacagaca acggataacg gtaagcagaa cacctatttc + 15301 tcctcactgg ataacatggt tgcccagggc aatgttctgc ctgttctgta cggggaaatg + 15361 cgcgtggggt cacgcgtggt ttctcaggag atcagcacgg cagacgaagg ggacggtggt + 15421 caggttgtgg tgattggtcg ctgatgcaaa atgttttatg tgaaaccgcc tgcgggcggt + 15481 tttgtcattt atggagcgtg aggaatgggt aaaggaagca gtaaggggca taccccgcgc + 15541 gaagcgaagg acaacctgaa gtccacgcag ttgctgagtg tgatcgatgc catcagcgaa + 15601 gggccgattg aaggtccggt ggatggctta aaaagcgtgc tgctgaacag tacgccggtg + 15661 ctggacactg aggggaatac caacatatcc ggtgtcacgg tggtgttccg ggctggtgag + 15721 caggagcaga ctccgccgga gggatttgaa tcctccggct ccgagacggt gctgggtacg + 15781 gaagtgaaat atgacacgcc gatcacccgc accattacgt ctgcaaacat cgaccgtctg + 15841 cgctttacct tcggtgtaca ggcactggtg gaaaccacct caaagggtga caggaatccg + 15901 tcggaagtcc gcctgctggt tcagatacaa cgtaacggtg gctgggtgac ggaaaaagac + 15961 atcaccatta agggcaaaac cacctcgcag tatctggcct cggtggtgat gggtaacctg + 16021 ccgccgcgcc cgtttaatat ccggatgcgc aggatgacgc cggacagcac cacagaccag + 16081 ctgcagaaca aaacgctctg gtcgtcatac actgaaatca tcgatgtgaa acagtgctac + 16141 ccgaacacgg cactggtcgg cgtgcaggtg gactcggagc agttcggcag ccagcaggtg + 16201 agccgtaatt atcatctgcg cgggcgtatt ctgcaggtgc cgtcgaacta taacccgcag + 16261 acgcggcaat acagcggtat ctgggacgga acgtttaaac cggcatacag caacaacatg + 16321 gcctggtgtc tgtgggatat gctgacccat ccgcgctacg gcatggggaa acgtcttggt + 16381 gcggcggatg tggataaatg ggcgctgtat gtcatcggcc agtactgcga ccagtcagtg + 16441 ccggacggct ttggcggcac ggagccgcgc atcacctgta atgcgtacct gaccacacag + 16501 cgtaaggcgt gggatgtgct cagcgatttc tgctcggcga tgcgctgtat gccggtatgg + 16561 aacgggcaga cgctgacgtt cgtgcaggac cgaccgtcgg ataagacgtg gacctataac + 16621 cgcagtaatg tggtgatgcc ggatgatggc gcgccgttcc gctacagctt cagcgccctg + 16681 aaggaccgcc ataatgccgt tgaggtgaac tggattgacc cgaacaacgg ctgggagacg + 16741 gcgacagagc ttgttgaaga tacgcaggcc attgcccgtt acggtcgtaa tgttacgaag + 16801 atggatgcct ttggctgtac cagccggggg caggcacacc gcgccgggct gtggctgatt + 16861 aaaacagaac tgctggaaac gcagaccgtg gatttcagcg tcggcgcaga agggcttcgc + 16921 catgtaccgg gcgatgttat tgaaatctgc gatgatgact atgccggtat cagcaccggt + 16981 ggtcgtgtgc tggcggtgaa cagccagacc cggacgctga cgctcgaccg tgaaatcacg + 17041 ctgccatcct ccggtaccgc gctgataagc ctggttgacg gaagtggcaa tccggtcagc + 17101 gtggaggttc agtccgtcac cgacggcgtg aaggtaaaag tgagccgtgt tcctgacggt + 17161 gttgctgaat acagcgtatg ggagctgaag ctgccgacgc tgcgccagcg actgttccgc + 17221 tgcgtgagta tccgtgagaa cgacgacggc acgtatgcca tcaccgccgt gcagcatgtg + 17281 ccggaaaaag aggccatcgt ggataacggg gcgcactttg acggcgaaca gagtggcacg + 17341 gtgaatggtg tcacgccgcc agcggtgcag cacctgaccg cagaagtcac tgcagacagc + 17401 ggggaatatc aggtgctggc gcgatgggac acaccgaagg tggtgaaggg cgtgagtttc + 17461 ctgctccgtc tgaccgtaac agcggacgac ggcagtgagc ggctggtcag cacggcccgg + 17521 acgacggaaa ccacataccg cttcacgcaa ctggcgctgg ggaactacag gctgacagtc + 17581 cgggcggtaa atgcgtgggg gcagcagggc gatccggcgt cggtatcgtt ccggattgcc + 17641 gcaccggcag caccgtcgag gattgagctg acgccgggct attttcagat aaccgccacg + 17701 ccgcatcttg ccgtttatga cccgacggta cagtttgagt tctggttctc ggaaaagcag + 17761 attgcggata tcagacaggt tgaaaccagc acgcgttatc ttggtacggc gctgtactgg + 17821 atagccgcca gtatcaatat caaaccgggc catgattatt acttttatat ccgcagtgtg + 17881 aacaccgttg gcaaatcggc attcgtggag gccgtcggtc gggcgagcga tgatgcggaa + 17941 ggttacctgg attttttcaa aggcaagata accgaatccc atctcggcaa ggagctgctg + 18001 gaaaaagtcg agctgacgga ggataacgcc agcagactgg aggagttttc gaaagagtgg + 18061 aaggatgcca gtgataagtg gaatgccatg tgggctgtca aaattgagca gaccaaagac + 18121 ggcaaacatt atgtcgcggg tattggcctc agcatggagg acacggagga aggcaaactg + 18181 agccagtttc tggttgccgc caatcgtatc gcatttattg acccggcaaa cgggaatgaa + 18241 acgccgatgt ttgtggcgca gggcaaccag atattcatga acgacgtgtt cctgaagcgc + 18301 ctgacggccc ccaccattac cagcggcggc aatcctccgg ccttttccct gacaccggac + 18361 ggaaagctga ccgctaaaaa tgcggatatc agtggcagtg tgaatgcgaa ctccgggacg + 18421 ctcagtaatg tgacgatagc tgaaaactgt acgataaacg gtacgctgag ggcggaaaaa + 18481 atcgtcgggg acattgtaaa ggcggcgagc gcggcttttc cgcgccagcg tgaaagcagt + 18541 gtggactggc cgtcaggtac ccgtactgtc accgtgaccg atgaccatcc ttttgatcgc + 18601 cagatagtgg tgcttccgct gacgtttcgc ggaagtaagc gtactgtcag cggcaggaca + 18661 acgtattcga tgtgttatct gaaagtactg atgaacggtg cggtgattta tgatggcgcg + 18721 gcgaacgagg cggtacaggt gttctcccgt attgttgaca tgccagcggg tcggggaaac + 18781 gtgatcctga cgttcacgct tacgtccaca cggcattcgg cagatattcc gccgtatacg + 18841 tttgccagcg atgtgcaggt tatggtgatt aagaaacagg cgctgggcat cagcgtggtc + 18901 tgagtgtgtt acagaggttc gtccgggaac gggcgtttta ttataaaaca gtgagaggtg + 18961 aacgatgcgt aatgtgtgta ttgccgttgc tgtctttgcc gcacttgcgg tgacagtcac + 19021 tccggcccgt gcggaaggtg gacatggtac gtttacggtg ggctattttc aagtgaaacc + 19081 gggtacattg ccgtcgttgt cgggcgggga taccggtgtg agtcatctga aagggattaa + 19141 cgtgaagtac cgttatgagc tgacggacag tgtgggggtg atggcttccc tggggttcgc + 19201 cgcgtcgaaa aagagcagca cagtgatgac cggggaggat acgtttcact atgagagcct + 19261 gcgtggacgt tatgtgagcg tgatggccgg accggtttta caaatcagta agcaggtcag + 19321 tgcgtacgcc atggccggag tggctcacag tcggtggtcc ggcagtacaa tggattaccg + 19381 taagacggaa atcactcccg ggtatatgaa agagacgacc actgccaggg acgaaagtgc + 19441 aatgcggcat acctcagtgg cgtggagtgc aggtatacag attaatccgg cagcgtccgt + 19501 cgttgttgat attgcttatg aaggctccgg cagtggcgac tggcgtactg acggattcat + 19561 cgttggggtc ggttataaat tctgattagc caggtaacac agtgttatga cagcccgccg + 19621 gaaccggtgg gcttttttgt ggggtgaata tggcagtaaa gatttcagga gtcctgaaag + 19681 acggcacagg aaaaccggta cagaactgca ccattcagct gaaagccaga cgtaacagca + 19741 ccacggtggt ggtgaacacg gtgggctcag agaatccgga tgaagccggg cgttacagca + 19801 tggatgtgga gtacggtcag tacagtgtca tcctgcaggt tgacggtttt ccaccatcgc + 19861 acgccgggac catcaccgtg tatgaagatt cacaaccggg gacgctgaat gattttctct + 19921 gtgccatgac ggaggatgat gcccggccgg aggtgctgcg tcgtcttgaa ctgatggtgg + 19981 aagaggtggc gcgtaacgcg tccgtggtgg cacagagtac ggcagacgcg aagaaatcag + 20041 ccggcgatgc cagtgcatca gctgctcagg tcgcggccct tgtgactgat gcaactgact + 20101 cagcacgcgc cgccagcacg tccgccggac aggctgcatc gtcagctcag gaagcgtcct + 20161 ccggcgcaga agcggcatca gcaaaggcca ctgaagcgga aaaaagtgcc gcagccgcag + 20221 agtcctcaaa aaacgcggcg gccaccagtg ccggtgcggc gaaaacgtca gaaacgaatg + 20281 ctgcagcgtc acaacaatca gccgccacgt ctgcctccac cgcggccacg aaagcgtcag + 20341 aggccgccac ttcagcacga gatgcggtgg cctcaaaaga ggcagcaaaa tcatcagaaa + 20401 cgaacgcatc atcaagtgcc ggtcgtgcag cttcctcggc aacggcggca gaaaattctg + 20461 ccagggcggc aaaaacgtcc gagacgaatg ccaggtcatc tgaaacagca gcggaacgga + 20521 gcgcctctgc cgcggcagac gcaaaaacag cggcggcggg gagtgcgtca acggcatcca + 20581 cgaaggcgac agaggctgcg ggaagtgcgg tatcagcatc gcagagcaaa agtgcggcag + 20641 aagcggcggc aatacgtgca naaaattcgg caaaacgtgc agaagatata gcttcagctg + 20701 tcgcgcttga ggatgcggac acaacgagaa aggggatagt gcagctcagc agtgcaacca + 20761 acagcacgtc tgaaacgctt gctgcaacgc caaaggcggt taaggtggta atggatgaaa + 20821 cgaacagaaa agcccactgg acagtccggc actgaccgga acgccaacag caccaaccgc + 20881 gctcagggga acaaacaata cccagattgc gaacaccgct tttgtactgg ccgcgattgc + 20941 agatgttatc gacgcgtcac ctgacgcact gaatacgctg aatgaactgg ccgcagcgct + 21001 cgggaatgat ccagattttg ctaccaccat gactaacgcg cttgcgggta aacaaccgaa + 21061 gaatgcgaca ctgacggcgc tggcagggct ttccacggcg aaaaataaat taccgtattt + 21121 tgcggaaaat gatgccgcca gcctgactga actgactcag gttggcaggg atattctggc + 21181 aaaaaattcc gttgcagatg ttcttgaata ccttggggcc ggtgagaatt cggcctttcc + 21241 ggcaggtgcg ccgatcccgt ggccatcaga tatcgttccg tctggctacg tcctgatgca + 21301 ggggcaggcg tttgacaaat cagcctaccc aaaacttgct gtcgcgtatc catcgggtgt + 21361 gcttcctgat atgcgaggct ggacaatcaa ggggaaaccc gccagcggtc gtgctgtatt + 21421 gtctcaggaa caggatggaa ttaagtcgca cacccacagt gccagtgcat ccggtacgga + 21481 tttggggacg aaaaccacat cgtcgtttga ttacgggacg aaaacaacag gcagtttcga + 21541 ttacggcacc aaatcgacga ataacacggg ggctcatgct cacagtctga gcggttcaac + 21601 aggggccgcg ggtgctcatg cccacacaag tggtttaagg atgaacagtt ctggctggag + 21661 tcagtatgga acagcaacca ttacaggaag tttatccaca gttaaaggaa ccagcacaca + 21721 gggtattgct tatttatcga aaacggacag tcagggcagc cacagtcact cattgtccgg + 21781 tacagccgtg agtgccggtg cacatgcgca tacagttggt attggtgcgc accagcatcc + 21841 ggttgttatc ggtgctcatg cccattcttt cagtattggt tcacacggac acaccatcac + 21901 cgttaacgct gcgggtaacg cggaaaacac cgtcaaaaac attgcattta actatattgt + 21961 gaggcttgca taatggcatt cagaatgagt gaacaaccac ggaccataaa aatttataat + 22021 ctgctggccg gaactaatga atttattggt gaaggtgacg catatattcc gcctcatacc + 22081 ggtctgcctg caaacagtac cgatattgca ccgccagata ttccggctgg ctttgtggct + 22141 gttttcaaca gtgatgaggc atcgtggcat ctcgttgaag accatcgggg taaaaccgtc + 22201 tatgacgtgg cttccggcga cgcgttattt atttctgaac tcggtccgtt accggaaaat + 22261 tttacctggt tatcgccggg aggggaatat cagaagtgga acggcacagc ctgggtgaag + 22321 gatacggaag cagaaaaact gttccggatc cgggaggcgg aagaaacaaa aaaaagcctg + 22381 atgcaggtag ccagtgagca tattgcgccg cttcaggatg ctgcagatct ggaaattgca + 22441 acgaaggaag aaacctcgtt gctggaagcc tggaagaagt atcgggtgtt gctgaaccgt + 22501 gttgatacat caactgcacc tgatattgag tggcctgctg tccctgttat ggagtaatcg + 22561 ttttgtgata tgccgcagaa acgttgtatg aaataacgtt ctgcggttag ttagtatatt + 22621 gtaaagctga gtattggttt atttggcgat tattatcttc aggagaataa tggaagttct + 22681 atgactcaat tgttcatagt gtttacatca ccgccaattg cttttaagac tgaacgcatg + 22741 aaatatggtt tttcgtcatg ttttgagtct gctgttgata tttctaaagt cggttttttt + 22801 tcttcgtttt ctctaactat tttccatgaa atacattttt gattattatt tgaatcaatt + 22861 ccaattacct gaagtctttc atctataatt ggcattgtat gtattggttt attggagtag + 22921 atgcttgctt ttctgagcca tagctctgat atccaaatga agccataggc atttgttatt + 22981 ttggctctgt cagctgcata acgccaaaaa atatatttat ctgcttgatc ttcaaatgtt + 23041 gtattgatta aatcaattgg atggaattgt ttatcataaa aaattaatgt ttgaatgtga + 23101 taaccgtcct ttaaaaaagt cgtttctgca agcttggctg tatagtcaac taactcttct + 23161 gtcgaagtga tatttttagg cttatctacc agttttagac gctctttaat atcttcagga + 23221 attattttat tgtcatattg tatcatgcta aatgacaatt tgcttatgga gtaatctttt + 23281 aattttaaat aagttattct cctggcttca tcaaataaag agtcgaatga tgttggcgaa + 23341 atcacatcgt cacccattgg attgtttatt tgtatgccaa gagagttaca gcagttatac + 23401 attctgccat agattatagc taaggcatgt aataattcgt aatcttttag cgtattagcg + 23461 acccatcgtc tttctgattt aataatagat gattcagtta aatatgaagg taatttcttt + 23521 tgtgcaagtc tgactaactt ttttatacca atgtttaaca tactttcatt tgtaataaac + 23581 tcaatgtcat tttcttcaat gtaagatgaa ataagagtag cctttgcctc gctatacatt + 23641 tctaaatcgc cttgtttttc tatcgtattg cgagaatttt tagcccaagc cattaatgga + 23701 tcatttttcc atttttcaat aacattattg ttataccaaa tgtcatatcc tataatctgg + 23761 tttttgtttt tttgaataat aaatgttact gttcttgcgg tttggaggaa ttgattcaaa + 23821 ttcaagcgaa ataattcagg gtcaaaatat gtatcaatgc agcatttgag caagtgcgat + 23881 aaatctttaa gtcttctttc ccatggtttt ttagtcataa aactctccat tttgataggt + 23941 tgcatgctag atgctgatat attttagagg tgataaaatt aactgcttaa ctgtcaatgt + 24001 aatacaagtt gtttgatctt tgcaatgatt cttatcagaa accatatagt aaattagtta + 24061 cacaggaaat ttttaatatt attattatca ttcattatgt attaaaatta gagttgtggc + 24121 ttggctctgc taacacgttg ctcataggag atatggtaga gccgcagaca cgtcgtatgc + 24181 aggaacgtgc tgcggctggc tggtgaactt ccgatagtgc gggtgttgaa tgatttccag + 24241 ttgctaccga ttttacatat tttttgcatg agagaatttg taccacctcc caccgaccat + 24301 ctatgactgt acgccactgt ccctaggact gctatgtgcc ggagcggaca ttacaaacgt + 24361 ccttctcggt gcatgccact gttgccaatg acctgcctag gaattggtta gcaagttact + 24421 accggatttt gtaaaaacag ccctcctcat ataaaaagta ttcgttcact tccgataagc + 24481 gtcgtaattt tctatctttc atcatattct agatccctct gaaaaaatct tccgagtttg + 24541 ctaggcactg atacataact cttttccaat aattggggaa gtcattcaaa tctataatag + 24601 gtttcagatt tgcttcaata aattctgact gtagctgctg aaacgttgcg gttgaactat + 24661 atttccttat aacttttacg aaagagtttc tttgagtaat cacttcactc aagtgcttcc + 24721 ctgcctccaa acgatacctg ttagcaatat ttaatagctt gaaatgatga agagctctgt + 24781 gtttgtcttc ctgcctccag ttcgccgggc attcaacata aaaactgata gcacccggag + 24841 ttccggaaac gaaatttgca tatacccatt gctcacgaaa aaaaatgtcc ttgtcgatat + 24901 agggatgaat cgcttggtgt acctcatcta ctgcgaaaac ttgacctttc tctcccatat + 24961 tgcagtcgcg gcacgatgga actaaattaa taggcatcac cgaaaattca ggataatgtg + 25021 caataggaag aaaatgatct atattttttg tctgtcctat atcaccacaa aatggacatt + 25081 tttcacctga tgaaacaagc atgtcatcgt aatatgttct agcgggtttg tttttatctc + 25141 ggagattatt ttcataaagc ttttctaatt taacctttgt caggttacca actactaagg + 25201 ttgtaggctc aagagggtgt gtcctgtcgt aggtaaataa ctgacctgtc gagcttaata + 25261 ttctatattg ttgttctttc tgcaaaaaag tggggaagtg agtaatgaaa ttatttctaa + 25321 catttatctg catcatacct tccgagcatt tattaagcat ttcgctataa gttctcgctg + 25381 gaagaggtag ttttttcatt gtactttacc ttcatctctg ttcattatca tcgcttttaa + 25441 aacggttcga ccttctaatc ctatctgacc attataattt tttagaatgg tttcataaga + 25501 aagctctgaa tcaacggact gcgataataa gtggtggtat ccagaatttg tcacttcaag + 25561 taaaaacacc tcacgagtta aaacacctaa gttctcaccg aatgtctcaa tatccggacg + 25621 gataatattt attgcttctc ttgaccgtag gactttccac atgcaggatt ttggaacctc + 25681 ttgcagtact actggggaat gagttgcaat tattgctaca ccattgcgtg catcgagtaa + 25741 gtcgcttaat gttcgtaaaa aagcagagag caaaggtgga tgcagatgaa cctctggttc + 25801 atcgaataaa actaatgact tttcgccaac gacatctact aatcttgtga tagtaaataa + 25861 aacaattgca tgtccagagc tcattcgaag cagatatttc tggatattgt cataaaacaa + 25921 tttagtgaat ttatcatcgt ccacttgaat ctgtggttca ttacgtctta actcttcata + 25981 tttagaaatg aggctgatga gttccatatt tgaaaagttt tcatcactac ttagtttttt + 26041 gatagcttca agccagagtt gtctttttct atctactctc atacaaccaa taaatgctga + 26101 aatgaattct aagcggagat cgcctagtga ttttaaacta ttgctggcag cattcttgag + 26161 tccaatataa aagtattgtg taccttttgc tgggtcaggt tgttctttag gaggagtaaa + 26221 aggatcaaat gcactaaacg aaactgaaac aagcgatcga aaatatccct ttgggattct + 26281 tgactcgata agtctattat tttcagagaa aaaatattca ttgttttctg ggttggtgat + 26341 tgcaccaatc attccattca aaattgttgt tttaccacac ccattccgcc cgataaaagc + 26401 atgaatgttc gtgctgggca tagaattaac cgtcacctca aaaggtatag ttaaatcact + 26461 gaatccggga gcactttttc tattaaatga aaagtggaaa tctgacaatt ctggcaaacc + 26521 atttaacaca cgtgcgaact gtccatgaat ttctgaaaga gttacccctc taagtaatga + 26581 ggtgttaagg acgctttcat tttcaatgtc ggctaatcga tttggccata ctactaaatc + 26641 ctgaatagct ttaagaaggt tatgtttaaa accatcgctt aatttgctga gattaacata + 26701 gtagtcaatg ctttcaccta aggaaaaaaa catttcaggg agttgactga attttttatc + 26761 tattaatgaa taagtgctta cttcttcttt ttgacctaca aaaccaattt taacatttcc + 26821 gatatcgcat ttttcaccat gctcatcaaa gacagtaaga taaaacattg taacaaagga + 26881 atagtcattc caaccatctg ctcgtaggaa tgccttattt ttttctactg caggaatata + 26941 cccgcctctt tcaataacac taaactccaa catatagtaa cccttaattt tattaaaata + 27001 accgcaattt atttggcggc aacacaggat ctctctttta agttactctc tattacatac + 27061 gttttccatc taaaaattag tagtattgaa cttaacgggg catcgtattg tagttttcca + 27121 tatttagctt tctgcttcct tttggataac ccactgttat tcatgttgca tggtgcactg + 27181 tttataccaa cgatatagtc tattaatgca tatatagtat cgccgaacga ttagctcttc + 27241 aggcttctga agaagcgttt caagtactaa taagccgata gatagccacg gacttcgtag + 27301 ccatttttca taagtgttaa cttccgctcc tcgctcataa cagacattca ctacagttat + 27361 ggcggaaagg tatgcatgct gggtgtgggg aagtcgtgaa agaaaagaag tcagctgcgt + 27421 cgtttgacat cactgctatc ttcttactgg ttatgcaggt cgtagtgggt ggcacacaaa + 27481 gctttgcact ggattgcgag gctttgtgct tctctggagt gcgacaggtt tgatgacaaa + 27541 aaattagcgc aagaagacaa aaatcacctt gcgctaatgc tctgttacag gtcactaata + 27601 ccatctaagt agttgattca tagtgactgc atatgttgtg ttttacagta ttatgtagtc + 27661 tgttttttat gcaaaatcta atttaatata ttgatattta tatcatttta cgtttctcgt + 27721 tcagcttttt tatactaagt tggcattata aaaaagcatt gcttatcaat ttgttgcaac + 27781 gaacaggtca ctatcagtca aaataaaatc attatttgat ttcaattttg tcccactccc + 27841 tgcctctgtc atcacgatac tgtgatgcca tggtgtccga cttatgcccg agaagatgtt + 27901 gagcaaactt atcgcttatc tgcttctcat agagtcttgc agacaaactg cgcaactcgt + 27961 gaaaggtagg cggatcccct tcgaaggaaa gacctgatgc ttttcgtgcg cgcataaaat + 28021 accttgatac tgtgccggat gaaagcggtt cgcgacgagt agatgcaatt atggtttctc + 28081 cgccaagaat ctctttgcat ttatcaagtg tttccttcat tgatattccg agagcatcaa + 28141 tatgcaatgc tgttgggatg gcaattttta cgcctgtttt gctttgctcg acataaagat + 28201 atccatctac gatatcagac cacttcattt cgcataaatc accaactcgt tgcccggtaa + 28261 caacagccag ttccattgca agtctgagcc aacatggtga tgattctgct gcttgataaa + 28321 ttttcaggta ttcgtcagcc gtaagtcttg atctccttac ctctgatttt gctgcgcgag + 28381 tggcagcgac atggtttgtt gttatatggc cttcagctat tgcctctcgg aatgcatcgc + 28441 tcagtgttga tctgattaac ttggctgacg ccgccttgcc ctcgtctatg tatccattga + 28501 gcattgccgc aatttctttt gtggtgatgt cttcaagtgg agcatcaggc agacccctcc + 28561 ttattgcttt aattttgctc atgtaattta tgagtgtctt ctgcttgatt cctctgctgg + 28621 ccaggatttt ttcgtagcga tcaagccatg aatgtaacgt aacggaatta tcactgttga + 28681 ttctcgctgt cagaggcttg tgtttgtgtc ctgaaaataa ctcaatgttg gcctgtatag + 28741 cttcagtgat tgcgattcgc ctgtctctgc ctaatccaaa ctctttaccc gtccttgggt + 28801 ccctgtagca gtaatatcca ttgtttctta tataaaggtt agggggtaaa tcccggcgct + 28861 catgacttcg ccttcttccc atttctgatc ctcttcaaaa ggccacctgt tactggtcga + 28921 tttaagtcaa cctttaccgc tgattcgtgg aacagatact ctcttccatc cttaaccgga + 28981 ggtgggaata tcctgcattc ccgaacccat cgacgaactg tttcaaggct tcttggacgt + 29041 cgctggcgtg cgttccactc ctgaagtgtc aagtacatcg caaagtctcc gcaattacac + 29101 gcaagaaaaa accgccatca ggcggcttgg tgttctttca gttcttcaat tcgaatattg + 29161 gttacgtctg catgtgctat ctgcgcccat atcatccagt ggtcgtagca gtcgttgatg + 29221 ttctccgctt cgataactct gttgaatggc tctccattcc attctcctgt gactcggaag + 29281 tgcatttatc atctccataa aacaaaaccc gccgtagcga gttcagataa aataaatccc + 29341 cgcgagtgcg aggattgtta tgtaatattg ggtttaatca tctatatgtt ttgtacagag + 29401 agggcaagta tcgtttccac cgtactcgtg ataataattt tgcacggtat cagtcatttc + 29461 tcgcacattg cagaatgggg atttgtcttc attagactta taaaccttca tggaatattt + 29521 gtatgccgac tctatatcta taccttcatc tacataaaca ccttcgtgat gtctgcatgg + 29581 agacaagaca ccggatctgc acaacattga taacgcccaa tctttttgct cagactctaa + 29641 ctcattgata ctcatttata aactccttgc aatgtatgtc gtttcagcta aacggtatca + 29701 gcaatgttta tgtaaagaaa cagtaagata atactcaacc cgatgtttga gtacggtcat + 29761 catctgacac tacagactct ggcatcgctg tgaagacgac gcgaaattca gcattttcac + 29821 aagcgttatc ttttacaaaa ccgatctcac tctcctttga tgcgaatgcc agcgtcagac + 29881 atcatatgca gatactcacc tgcatcctga acccattgac ctccaacccc gtaatagcga + 29941 tgcgtaatga tgtcgatagt tactaacggg tcttgttcga ttaactgccg cagaaactct + 30001 tccaggtcac cagtgcagtg cttgataaca ggagtcttcc caggatggcg aacaacaaga + 30061 aactggtttc cgtcttcacg gacttcgttg ctttccagtt tagcaatacg cttactccca + 30121 tccgagataa caccttcgta atactcacgc tgctcgttga gttttgattt tgctgtttca + 30181 agctcaacac gcagtttccc tactgttagc gcaatatcct cgttctcctg gtcgcggcgt + 30241 ttgatgtatt gctggtttct ttcccgttca tccagcagtt ccagcacaat cgatggtgtt + 30301 accaattcat ggaaaaggtc tgcgtcaaat ccccagtcgt catgcattgc ctgctctgcc + 30361 gcttcacgca gtgcctgaga gttaatttcg ctcacttcga acctctctgt ttactgataa + 30421 gttccagatc ctcctggcaa cttgcacaag tccgacaacc ctgaacgacc aggcgtcttc + 30481 gttcatctat cggatcgcca cactcacaac aatgagtggc agatatagcc tggtggttca + 30541 ggcggcgcat ttttattgct gtgttgcgct gtaattcttc tatttctgat gctgaatcaa + 30601 tgatgtctgc catctttcat taatccctga actgttggtt aatacgcttg agggtgaatg + 30661 cgaataataa aaaaggagcc tgtagctccc tgatgatttt gcttttcatg ttcatcgttc + 30721 cttaaagacg ccgtttaaca tgccgattgc caggcttaaa tgagtcggtg tgaatcccat + 30781 cagcgttacc gtttcgcggt gcttcttcag tacgctacgg caaatgtcat cgacgttttt + 30841 atccggaaac tgctgtctgg ctttttttga tttcagaatt agcctgacgg gcaatgctgc + 30901 gaagggcgtt ttcctgctga ggtgtcattg aacaagtccc atgtcggcaa gcataagcac + 30961 acagaatatg aagcccgctg ccagaaaaat gcattccgtg gttgtcatac ctggtttctc + 31021 tcatctgctt ctgctttcgc caccatcatt tccagctttt gtgaaaggga tgcggctaac + 31081 gtatgaaatt cttcgtctgt ttctactggt attggcacaa acctgattcc aatttgagca + 31141 aggctatgtg ccatctcgat actcgttctt aactcaacag aagatgcttt gtgcatacag + 31201 cccctcgttt attatttatc tcctcagcca gccgctgtgc tttcagtgga tttcggataa + 31261 cagaaaggcc gggaaatacc cagcctcgct ttgtaacgga gtagacgaaa gtgattgcgc + 31321 ctacccggat attatcgtga ggatgcgtca tcgccattgc tccccaaata caaaaccaat + 31381 ttcagccagt gcctcgtcca ttttttcgat gaactccggc acgatctcgt caaaactcgc + 31441 catgtacttt tcatcccgct caatcacgac ataatgcagg ccttcacgct tcatacgcgg + 31501 gtcatagttg gcaaagtacc aggcattttt tcgcgtcacc cacatgctgt actgcacctg + 31561 ggccatgtaa gctgacttta tggcctcgaa accaccgagc cggaacttca tgaaatcccg + 31621 ggaggtaaac gggcatttca gttcaaggcc gttgccgtca ctgcataaac catcgggaga + 31681 gcaggcggta cgcatacttt cgtcgcgata gatgatcggg gattcagtaa cattcacgcc + 31741 ggaagtgaat tcaaacaggg ttctggcgtc gttctcgtac tgttttcccc aggccagtgc + 31801 tttagcgtta acttccggag ccacaccggt gcaaacctca gcaagcaggg tgtggaagta + 31861 ggacattttc atgtcaggcc acttctttcc ggagcggggt tttgctatca cgttgtgaac + 31921 ttctgaagcg gtgatgacgc cgagccgtaa tttgtgccac gcatcatccc cctgttcgac + 31981 agctctcaca tcgatcccgg tacgctgcag gataatgtcc ggtgtcatgc tgccaccttc + 32041 tgctctgcgg ctttctgttt caggaatcca agagctttta ctgcttcggc ctgtgtcagt + 32101 tctgacgatg cacgaatgtc gcggcgaaat atctgggaac agagcggcaa taagtcgtca + 32161 tcccatgttt tatccagggc gatcagcaga gtgttaatct cctgcatggt ttcatcgtta + 32221 accggagtga tgtcgcgttc cggctgacgt tctgcagtgt atgcagtatt ttcgacaatg + 32281 cgctcggctt catccttgtc atagatacca gcaaatccga aggccagacg ggcacactga + 32341 atcatggctt tatgacgtaa catccgtttg ggatgcgact gccacggccc cgtgatttct + 32401 ctgccttcgc gagttttgaa tggttcgcgg cggcattcat ccatccattc ggtaacgcag + 32461 atcggatgat tacggtcctt gcggtaaatc cggcatgtac aggattcatt gtcctgctca + 32521 aagtccatgc catcaaactg ctggttttca ttgatgatgc gggaccagcc atcaacgccc + 32581 accaccggaa cgatgccatt ctgcttatca ggaaaggcgt aaatttcttt cgtccacgga + 32641 ttaaggccgt actggttggc aacgatcagt aatgcgatga actgcgcatc gctggcatca + 32701 cctttaaatg ccgtctggcg aagagtggtg atcagttcct gtgggtcgac agaatccatg + 32761 ccgacacgtt cagccagctt cccagccagc gttgcgagtg cagtactcat tcgttttata + 32821 cctctgaatc aatatcaacc tggtggtgag caatggtttc aaccatgtac cggatgtgtt + 32881 ctgccatgcg ctcctgaaac tcaacatcgt catcaaacgc acgggtaatg gattttttgc + 32941 tggccccgtg gcgttgcaaa tgatcgatgc atagcgattc aaacaggtgc tggggcaggc + 33001 ctttttccat gtcgtctgcc agttctgcct ctttctcttc acgggcgagc tgctggtagt + 33061 gacgcgccca gctctgagcc tcaagacgat cctgaatgta ataagcgttc atggctgaac + 33121 tcctgaaata gctgtgaaaa tatcgcccgc gaaatgccgg gctgattagg aaaacaggaa + 33181 agggggttag tgaatgcttt tgcttgatct cagtttcagt attaatatcc attttttata + 33241 agcgtcgacg gcttcacgaa acatcttttc atcgccaata aaagtggcga tagtgaattt + 33301 agtctggata gccataagtg tttgatccat tctttgggac tcctggctga ttaagtatgt + 33361 cgataaggcg tttccatccg tcacgtaatt tacgggtgat tcgttcaagt aaagattcgg + 33421 aagggcagcc agcaacaggc caccctgcaa tggcatattg catggtgtgc tccttattta + 33481 tacataacga aaaacgcctc gagtgaagcg ttattggtat gcggtaaaac cgcactcagg + 33541 cggccttgat agtcatatca tctgaatcaa atattcctga tgtatcgata tcggtaattc + 33601 ttattccttc gctaccatcc attggaggcc atccttcctg accatttcca tcattccagt + 33661 cgaactcaca cacaacacca tatgcattta agtcgcttga aattgctata agcagagcat + 33721 gttgcgccag catgattaat acagcattta atacagagcc gtgtttattg agtcggtatt + 33781 cagagtctga ccagaaatta ttaatctggt gaagtttttc ctctgtcatt acgtcatggt + 33841 cgatttcaat ttctattgat gctttccagt cgtaatcaat gatgtatttt ttgatgtttg + 33901 acatctgttc atatcctcac agataaaaaa tcgccctcac actggagggc aaagaagatt + 33961 tccaataatc agaacaagtc ggctcctgtt tagttacgag cgacattgct ccgtgtattc + 34021 actcgttgga atgaatacac agtgcagtgt ttattctgtt atttatgcca aaaataaagg + 34081 ccactatcag gcagctttgt tgttctgttt accaagttct ctggcaatca ttgccgtcgt + 34141 tcgtattgcc catttatcga catatttccc atcttccatt acaggaaaca tttcttcagg + 34201 cttaaccatg cattccgatt gcagcttgca tccattgcat cgcttgaatt gtccacacca + 34261 ttgattttta tcaatagtcg tagtcatacg gatagtcctg gtattgttcc atcacatcct + 34321 gaggatgctc ttcgaactct tcaaattctt cttccatata tcaccttaaa tagtggattg + 34381 cggtagtaaa gattgtgcct gtcttttaac cacatcaggc tcggtggttc tcgtgtaccc + 34441 ctacagcgag aaatcggata aactattaca acccctacag tttgatgagt atagaaatgg + 34501 atccactcgt tattctcgga cgagtgttca gtaatgaacc tctggagaga accatgtata + 34561 tgatcgttat ctgggttgga cttctgcttt taagcccaga taactggcct gaatatgtta + 34621 atgagagaat cggtattcct catgtgtggc atgttttcgt ctttgctctt gcattttcgc + 34681 tagcaattaa tgtgcatcga ttatcagcta ttgccagcgc cagatataag cgatttaagc + 34741 taagaaaacg cattaagatg caaaacgata aagtgcgatc agtaattcaa aaccttacag + 34801 aagagcaatc tatggttttg tgcgcagccc ttaatgaagg caggaagtat gtggttacat + 34861 caaaacaatt cccatacatt agtgagttga ttgagcttgg tgtgttgaac aaaacttttt + 34921 cccgatggaa tggaaagcat atattattcc ctattgagga tatttactgg actgaattag + 34981 ttgccagcta tgatccatat aatattgaga taaagccaag gccaatatct aagtaactag + 35041 ataagaggaa tcgattttcc cttaattttc tggcgtccac tgcatgttat gccgcgttcg + 35101 ccaggcttgc tgtaccatgt gcgctgattc ttgcgctcaa tacgttgcag gttgctttca + 35161 atctgtttgt ggtattcagc cagcactgta aggtctatcg gatttagtgc gctttctact + 35221 cgtgatttcg gtttgcgatt cagcgagaga atagggcggt taactggttt tgcgcttacc + 35281 ccaaccaaca ggggatttgc tgctttccat tgagcctgtt tctctgcgcg acgttcgcgg + 35341 cggcgtgttt gtgcatccat ctggattctc ctgtcagtta gctttggtgg tgtgtggcag + 35401 ttgtagtcct gaacgaaaac cccccgcgat tggcacattg gcagctaatc cggaatcgca + 35461 cttacggcca atgcttcgtt tcgtatcaca caccccaaag ccttctgctt tgaatgctgc + 35521 ccttcttcag ggcttaattt ttaagagcgt caccttcatg gtggtcagtg cgtcctgctg + 35581 atgtgctcag tatcaccgcc agtggtattt atgtcaacac cgccagagat aatttatcac + 35641 cgcagatggt tatctgtatg ttttttatat gaatttattt tttgcagggg ggcattgttt + 35701 ggtaggtgag agatctgaat tgctatgttt agtgagttgt atctatttat ttttcaataa + 35761 atacaattgg ttatgtgttt tgggggcgat cgtgaggcaa agaaaacccg gcgctgaggc + 35821 cgggttattc ttgttctctg gtcaaattat atagttggaa aacaaggatg catatatgaa + 35881 tgaacgatgc agaggcaatg ccgatggcga tagtgggtat catgtagccg cttatgctgg + 35941 aaagaagcaa taacccgcag aaaaacaaag ctccaagctc aacaaaacta agggcataga + 36001 caataactac cgatgtcata tacccatact ctctaatctt ggccagtcgg cgcgttctgc + 36061 ttccgattag aaacgtcaag gcagcaatca ggattgcaat catggttcct gcatatgatg + 36121 acaatgtcgc cccaagacca tctctatgag ctgaaaaaga aacaccagga atgtagtggc + 36181 ggaaaaggag atagcaaatg cttacgataa cgtaaggaat tattactatg taaacaccag + 36241 gcatgattct gttccgcata attactcctg ataattaatc cttaactttg cccacctgcc + 36301 ttttaaaaca ttccagtata tcacttttca ttcttgcgta gcaatatgcc atctcttcag + 36361 ctatctcagc attggtgacc ttgttcagag gcgctgagag atggcctttt tctgatagat + 36421 aatgttctgt taaaatatct ccggcctcat cttttgcccg caggctaatg tctgaaaatt + 36481 gaggtgacgg gttaaaaata atatccttgg caaccttttt tatatccctt ttaaattttg + 36541 gcttaatgac tatatccaat gagtcaaaaa gctccccttc aatatctgtt gcccctaaga + 36601 cctttaatat atcgccaaat acaggtagct tggcttctac cttcaccgtt gttcggccga + 36661 tgaaatgcat atgcataaca tcgtctttgg tggttcccct catcagtggc tctatctgaa + 36721 cgcgctctcc actgcttaat gacattcctt tcccgattaa aaaatctgtc agatcggatg + 36781 tggtcggccc gaaaacagtt ctggcaaaac caatggtgtc gccttcaaca aacaaaaaag + 36841 atgggaatcc caatgattcg tcatctgcga ggctgttctt aatatcttca actgaagctt + 36901 tagagcgatt tatcttctga accagactct tgtcatttgt tttggtaaag agaaaagttt + 36961 ttccatcgat tttatgaata tacaaataat tggagccaac ctgcaggtga tgattatcag + 37021 ccagcagaga attaaggaaa acagacaggt ttattgagcg cttatctttc cctttatttt + 37081 tgctgcggta agtcgcataa aaaccattct tcataattca atccatttac tatgttatgt + 37141 tctgagggga gtgaaaattc ccctaattcg atgaagattc ttgctcaatt gttatcagct + 37201 atgcgccgac cagaacacct tgccgatcag ccaaacgtct cttcaggcca ctgactagcg + 37261 ataactttcc ccacaacgga acaactctca ttgcatggga tcattgggta ctgtgggttt + 37321 agtggttgta aaaacacctg accgctatcc ctgatcagtt tcttgaaggt aaactcatca + 37381 cccccaagtc tggctatgca gaaatcacct ggctcaacag cctgctcagg gtcaacgaga + 37441 attaacattc cgtcaggaaa gcttggcttg gagcctgttg gtgcggtcat ggaattacct + 37501 tcaacctcaa gccagaatgc agaatcactg gcttttttgg ttgtgcttac ccatctctcc + 37561 gcatcacctt tggtaaaggt tctaagctca ggtgagaaca tccctgcctg aacatgagaa + 37621 aaaacagggt actcatactc acttctaagt gacggctgca tactaaccgc ttcatacatc + 37681 tcgtagattt ctctggcgat tgaagggcta aattcttcaa cgctaacttt gagaattttt + 37741 gcaagcaatg cggcgttata agcatttaat gcattgatgc cattaaataa agcaccaacg + 37801 cctgactgcc ccatccccat cttgtctgcg acagattcct gggataagcc aagttcattt + 37861 ttcttttttt cataaattgc tttaaggcga cgtgcgtcct caagctgctc ttgtgttaat + 37921 ggtttctttt ttgtgctcat acgttaaatc tatcaccgca agggataaat atctaacacc + 37981 gtgcgtgttg actattttac ctctggcggt gataatggtt gcatgtacta aggaggttgt + 38041 atggaacaac gcataaccct gaaagattat gcaatgcgct ttgggcaaac caagacagct + 38101 aaagatctcg gcgtatatca aagcgcgatc aacaaggcca ttcatgcagg ccgaaagatt + 38161 tttttaacta taaacgctga tggaagcgtt tatgcggaag aggtaaagcc cttcccgagt + 38221 aacaaaaaaa caacagcata aataaccccg ctcttacaca ttccagccct gaaaaagggc + 38281 atcaaattaa accacaccta tggtgtatgc atttatttgc atacattcaa tcaattgtta + 38341 tctaaggaaa tacttacata tggttcgtgc aaacaaacgc aacgaggctc tacgaatcga + 38401 gagtgcgttg cttaacaaaa tcgcaatgct tggaactgag aagacagcgg aagctgtggg + 38461 cgttgataag tcgcagatca gcaggtggaa gagggactgg attccaaagt tctcaatgct + 38521 gcttgctgtt cttgaatggg gggtcgttga cgacgacatg gctcgattgg cgcgacaagt + 38581 tgctgcgatt ctcaccaata aaaaacgccc ggcggcaacc gagcgttctg aacaaatcca + 38641 gatggagttc tgaggtcatt actggatcta tcaacaggag tcattatgac aaatacagca + 38701 aaaatactca acttcggcag aggtaacttt gccggacagg agcgtaatgt ggcagatctc + 38761 gatgatggtt acgccagact atcaaatatg ctgcttgagg cttattcggg cgcagatctg + 38821 accaagcgac agtttaaagt gctgcttgcc attctgcgta aaacctatgg gtggaataaa + 38881 ccaatggaca gaatcaccga ttctcaactt agcgagatta caaagttacc tgtcaaacgg + 38941 tgcaatgaag ccaagttaga actcgtcaga atgaatatta tcaagcagca aggcggcatg + 39001 tttggaccaa ataaaaacat ctcagaatgg tgcatccctc aaaacgaggg aaaatcccct + 39061 aaaacgaggg ataaaacatc cctcaaattg ggggattgct atccctcaaa acagggggac + 39121 acaaaagaca ctattacaaa agaaaaaaga aaagattatt cgtcagagaa ttctggcgaa + 39181 tcctctgacc agccagaaaa cgacctttct gtggtgaaac cggatgctgc aattcagagc + 39241 ggcagcaagt gggggacagc agaagacctg accgccgcag agtggatgtt tgacatggtg + 39301 aagactatcg caccatcagc cagaaaaccg aattttgctg ggtgggctaa cgatatccgc + 39361 ctgatgcgtg aacgtgacgg acgtaaccac cgcgacatgt gtgtgctgtt ccgctgggca + 39421 tgccaggaca acttctggtc cggtaacgtg ctgagcccgg ccaaactccg cgataagtgg + 39481 acccaactcg aaatcaaccg taacaagcaa caggcaggcg tgacagccag caaaccaaaa + 39541 ctcgacctga caaacacaga ctggatttac ggggtggatc tatgaaaaac atcgccgcac + 39601 agatggttaa ctttgaccgt gagcagatgc gtcggatcgc caacaacatg ccggaacagt + 39661 acgacgaaaa gccgcaggta cagcaggtag cgcagatcat caacggtgtg ttcagccagt + 39721 tactggcaac tttcccggcg agcctggcta accgtgacca gaacgaagtg aacgaaatcc + 39781 gtcgccagtg ggttctggct tttcgggaaa acgggatcac cacgatggaa caggttaacg + 39841 caggaatgcg cgtagcccgt cggcagaatc gaccatttct gccatcaccc gggcagtttg + 39901 ttgcatggtg ccgggaagaa gcatccgtta ccgccggact gccaaacgtc agcgagctgg + 39961 ttgatatggt ttacgagtat tgccggaagc gaggcctgta tccggatgcg gagtcttatc + 40021 cgtggaaatc aaacgcgcac tactggctgg ttaccaacct gtatcagaac atgcgggcca + 40081 atgcgcttac tgatgcggaa ttacgccgta aggccgcaga tgagcttgtc catatgactg + 40141 cgagaattaa ccgtggtgag gcgatccctg aaccagtaaa acaacttcct gtcatgggcg + 40201 gtagacctct aaatcgtgca caggctctgg cgaagatcgc agaaatcaaa gctaagttcg + 40261 gactgaaagg agcaagtgta tgacgggcaa agaggcaatt attcattacc tggggacgca + 40321 taatagcttc tgtgcgccgg acgttgccgc gctaacaggc gcaacagtaa ccagcataaa + 40381 tcaggccgcg gctaaaatgg cacgggcagg tcttctggtt atcgaaggta aggtctggcg + 40441 aacggtgtat taccggtttg ctaccaggga agaacgggaa ggaaagatga gcacgaacct + 40501 ggtttttaag gagtgtcgcc agagtgccgc gatgaaacgg gtattggcgg tatatggagt + 40561 taaaagatga ccatctacat tactgagcta ataacaggcc tgctggtaat cgcaggcctt + 40621 tttatttggg ggagagggaa gtcatgaaaa aactaacctt tgaaattcga tctccagcac + 40681 atcagcaaaa cgctattcac gcagtacagc aaatccttcc agacccaacc aaaccaatcg + 40741 tagtaaccat tcaggaacgc aaccgcagct tagaccaaaa caggaagcta tgggcctgct + 40801 taggtgacgt ctctcgtcag gttgaatggc atggtcgctg gctggatgca gaaagctgga + 40861 agtgtgtgtt taccgcagca ttaaagcagc aggatgttgt tcctaacctt gccgggaatg + 40921 gctttgtggt aataggccag tcaaccagca ggatgcgtgt aggcgaattt gcggagctat + 40981 tagagcttat acaggcattc ggtacagagc gtggcgttaa gtggtcagac gaagcgagac + 41041 tggctctgga gtggaaagcg agatggggag acagggctgc atgataaatg tcgttagttt + 41101 ctccggtggc aggacgtcag catatttgct ctggctaatg gagcaaaagc gacgggcagg + 41161 taaagacgtg cattacgttt tcatggatac aggttgtgaa catccaatga catatcggtt + 41221 tgtcagggaa gttgtgaagt tctgggatat accgctcacc gtattgcagg ttgatatcaa + 41281 cccggagctt ggacagccaa atggttatac ggtatgggaa ccaaaggata ttcagacgcg + 41341 aatgcctgtt ctgaagccat ttatcgatat ggtaaagaaa tatggcactc catacgtcgg + 41401 cggcgcgttc tgcactgaca gattaaaact cgttcccttc accaaatact gtgatgacca + 41461 tttcgggcga gggaattaca ccacgtggat tggcatcaga gctgatgaac cgaagcggct + 41521 aaagccaaag cctggaatca gatatcttgc tgaactgtca gactttgaga aggaagatat + 41581 cctcgcatgg tggaagcaac aaccattcga tttgcaaata ccggaacatc tcggtaactg + 41641 catattctgc attaaaaaat caacgcaaaa aatcggactt gcctgcaaag atgaggaggg + 41701 attgcagcgt gtttttaatg aggtcatcac gggatcccat gtgcgtgacg gacatcggga + 41761 aacgccaaag gagattatgt accgaggaag aatgtcgctg gacggtatcg cgaaaatgta + 41821 ttcagaaaat gattatcaag ccctgtatca ggacatggta cgagctaaaa gattcgatac + 41881 cggctcttgt tctgagtcat gcgaaatatt tggagggcag cttgatttcg acttcgggag + 41941 ggaagctgca tgatgcgatg ttatcggtgc ggtgaatgca aagaagataa ccgcttccga + 42001 ccaaatcaac cttactggaa tcgatggtgt ctccggtgtg aaagaacacc aacaggggtg + 42061 ttaccactac cgcaggaaaa ggaggacgtg tggcgagaca gcgacgaagt atcaccgaca + 42121 taatctgcga aaactgcaaa taccttccaa cgaaacgcac cagaaataaa cccaagccaa + 42181 tcccaaaaga atctgacgta aaaaccttca actacacggc tcacctgtgg gatatccggt + 42241 ggctaagacg tcgtgcgagg aaaacaaggt gattgaccaa aatcgaagtt acgaacaaga + 42301 aagcgtcgag cgagctttaa cgtgcgctaa ctgcggtcag aagctgcatg tgctggaagt + 42361 tcacgtgtgt gagcactgct gcgcagaact gatgagcgat ccgaatagct cgatgcacga + 42421 ggaagaagat gatggctaaa ccagcgcgaa gacgatgtaa aaacgatgaa tgccgggaat + 42481 ggtttcaccc tgcattcgct aatcagtggt ggtgctctcc agagtgtgga accaagatag + 42541 cactcgaacg acgaagtaaa gaacgcgaaa aagcggaaaa agcagcagag aagaaacgac + 42601 gacgagagga gcagaaacag aaagataaac ttaagattcg aaaactcgcc ttaaagcccc + 42661 gcagttactg gattaaacaa gcccaacaag ccgtaaacgc cttcatcaga gaaagagacc + 42721 gcgacttacc atgtatctcg tgcggaacgc tcacgtctgc tcagtgggat gccggacatt + 42781 accggacaac tgctgcggca cctcaactcc gatttaatga acgcaatatt cacaagcaat + 42841 gcgtggtgtg caaccagcac aaaagcggaa atctcgttcc gtatcgcgtc gaactgatta + 42901 gccgcatcgg gcaggaagca gtagacgaaa tcgaatcaaa ccataaccgc catcgctgga + 42961 ctatcgaaga gtgcaaggcg atcaaggcag agtaccaaca gaaactcaaa gacctgcgaa + 43021 atagcagaag tgaggccgca tgacgttctc agtaaaaacc attccagaca tgctcgttga + 43081 agcatacgga aatcagacag aagtagcacg cagactgaaa tgtagtcgcg gtacggtcag + 43141 aaaatacgtt gatgataaag acgggaaaat gcacgccatc gtcaacgacg ttctcatggt + 43201 tcatcgcgga tggagtgaaa gagatgcgct attacgaaaa aattgatggc agcaaatacc + 43261 gaaatatttg ggtagttggc gatctgcacg gatgctacac gaacctgatg aacaaactgg + 43321 atacgattgg attcgacaac aaaaaagacc tgcttatctc ggtgggcgat ttggttgatc + 43381 gtggtgcaga gaacgttgaa tgcctggaat taatcacatt cccctggttc agagctgtac + 43441 gtggaaacca tgagcaaatg atgattgatg gcttatcaga gcgtggaaac gttaatcact + 43501 ggctgcttaa tggcggtggc tggttcttta atctcgatta cgacaaagaa attctggcta + 43561 aagctcttgc ccataaagca gatgaacttc cgttaatcat cgaactggtg agcaaagata + 43621 aaaaatatgt tatctgccac gccgattatc cctttgacga atacgagttt ggaaagccag + 43681 ttgatcatca gcaggtaatc tggaaccgcg aacgaatcag caactcacaa aacgggatcg + 43741 tgaaagaaat caaaggcgcg gacacgttca tctttggtca tacgccagca gtgaaaccac + 43801 tcaagtttgc caaccaaatg tatatcgata ccggcgcagt gttctgcgga aacctaacat + 43861 tgattcaggt acagggagaa ggcgcatgag actcgaaagc gtagctaaat ttcattcgcc + 43921 aaaaagcccg atgatgagcg actcaccacg ggccacggct tctgactctc tttccggtac + 43981 tgatgtgatg gctgctatgg ggatggcgca atcacaagcc ggattcggta tggctgcatt + 44041 ctgcggtaag cacgaactca gccagaacga caaacaaaag gctatcaact atctgatgca + 44101 atttgcacac aaggtatcgg ggaaataccg tggtgtggca aagcttgaag gaaatactaa + 44161 ggcaaaggta ctgcaagtgc tcgcaacatt cgcttatgcg gattattgcc gtagtgccgc + 44221 gacgccgggg gcaagatgca gagattgcca tggtacaggc cgtgcggttg atattgccaa + 44281 aacagagctg tgggggagag ttgtcgagaa agagtgcgga agatgcaaag gcgtcggcta + 44341 ttcaaggatg ccagcaagcg cagcatatcg cgctgtgacg atgctaatcc caaaccttac + 44401 ccaacccacc tggtcacgca ctgttaagcc gctgtatgac gctctggtgg tgcaatgcca + 44461 caaagaagag tcaatcgcag acaacatttt gaatgcggtc acacgttagc agcatgattg + 44521 ccacggatgg caacatatta acggcatgat attgacttat tgaataaaat tgggtaaatt + 44581 tgactcaacg atgggttaat tcgctcgttg tggtagtgag atgaaaagag gcggcgctta + 44641 ctaccgattc cgcctagttg gtcacttcga cgtatcgtct ggaactccaa ccatcgcagg + 44701 cagagaggtc tgcaaaatgc aatcccgaaa cagttcgcag gtaatagtta gagcctgcat + 44761 aacggtttcg ggatttttta tatctgcaca acaggtaaga gcattgagtc gataatcgtg + 44821 aagagtcggc gagcctggtt agccagtgct ctttccgttg tgctgaatta agcgaatacc + 44881 ggaagcagaa ccggatcacc aaatgcgtac aggcgtcatc gccgcccagc aacagcacaa + 44941 cccaaactga gccgtagcca ctgtctgtcc tgaattcatt agtaatagtt acgctgcggc + 45001 cttttacaca tgaccttcgt gaaagcgggt ggcaggaggt cgcgctaaca acctcctgcc + 45061 gttttgcccg tgcatatcgg tcacgaacaa atctgattac taaacacagt agcctggatt + 45121 tgttctatca gtaatcgacc ttattcctaa ttaaatagag caaatcccct tattgggggt + 45181 aagacatgaa gatgccagaa aaacatgacc tgttggccgc cattctcgcg gcaaaggaac + 45241 aaggcatcgg ggcaatcctt gcgtttgcaa tggcgtacct tcgcggcaga tataatggcg + 45301 gtgcgtttac aaaaacagta atcgacgcaa cgatgtgcgc cattatcgcc tggttcattc + 45361 gtgaccttct cgacttcgcc ggactaagta gcaatctcgc ttatataacg agcgtgttta + 45421 tcggctacat cggtactgac tcgattggtt cgcttatcaa acgcttcgct gctaaaaaag + 45481 ccggagtaga agatggtaga aatcaataat caacgtaagg cgttcctcga tatgctggcg + 45541 tggtcggagg gaactgataa cggacgtcag aaaaccagaa atcatggtta tgacgtcatt + 45601 gtaggcggag agctatttac tgattactcc gatcaccctc gcaaacttgt cacgctaaac + 45661 ccaaaactca aatcaacagg cgccggacgc taccagcttc tttcccgttg gtgggatgcc + 45721 taccgcaagc agcttggcct gaaagacttc tctccgaaaa gtcaggacgc tgtggcattg + 45781 cagcagatta aggagcgtgg cgctttacct atgattgatc gtggtgatat ccgtcaggca + 45841 atcgaccgtt gcagcaatat ctgggcttca ctgccgggcg ctggttatgg tcagttcgag + 45901 cataaggctg acagcctgat tgcaaaattc aaagaagcgg gcggaacggt cagagagatt + 45961 gatgtatgag cagagtcacc gcgattatct ccgctctggt tatctgcatc atcgtctgcc + 46021 tgtcatgggc tgttaatcat taccgtgata acgccattac ctacaaagcc cagcgcgaca + 46081 aaaatgccag agaactgaag ctggcgaacg cggcaattac tgacatgcag atgcgtcagc + 46141 gtgatgttgc tgcgctcgat gcaaaataca cgaaggagtt agctgatgct aaagctgaaa + 46201 atgatgctct gcgtgatgat gttgccgctg gtcgtcgtcg gttgcacatc aaagcagtct + 46261 gtcagtcagt gcgtgaagcc accaccgcct ccggcgtgga taatgcagcc tccccccgac + 46321 tggcagacac cgctgaacgg gattatttca ccctcagaga gaggctgatc actatgcaaa + 46381 aacaactgga aggaacccag aagtatatta atgagcagtg cagatagagt tgcccatatc + 46441 gatgggcaac tcatgcaatt attgtgagca atacacacgc gcttccagcg gagtataaat + 46501 gcctaaagta ataaaaccga gcaatccatt tacgaatgtt tgctgggttt ctgttttaac + 46561 aacattttct gcgccgccac aaattttggc tgcatcgaca gttttcttct gcccaattcc + 46621 agaaacgaag aaatgatggg tgatggtttc ctttggtgct actgctgccg gtttgttttg + 46681 aacagtaaac gtctgttgag cacatcctgt aataagcagg gccagcgcag tagcgagtag + 46741 catttttttc atggtgttat tcccgatgct ttttgaagtt cgcagaatcg tatgtgtaga + 46801 aaattaaaca aaccctaaac aatgagttga aatttcatat tgttaatatt tattaatgta + 46861 tgtcaggtgc gatgaatcgt cattgtattc ccggattaac tatgtccaca gccctgacgg + 46921 ggaacttctc tgcgggagtg tccgggaata attaaaacga tgcacacagg gtttagcgcg + 46981 tacacgtatt gcattatgcc aacgccccgg tgctgacacg gaagaaaccg gacgttatga + 47041 tttagcgtgg aaagatttgt gtagtgttct gaatgctctc agtaaatagt aatgaattat + 47101 caaaggtata gtaatatctt ttatgttcat ggatatttgt aacccatcgg aaaactcctg + 47161 ctttagcaag attttccctg tattgctgaa atgtgatttc tcttgatttc aacctatcat + 47221 aggacgtttc tataagatgc gtgtttcttg agaatttaac atttacaacc tttttaagtc + 47281 cttttattaa cacggtgtta tcgttttcta acacgangtg aatattatct gtggctagat + 47341 agtaaatata atgtgagacg ttgtgacgtt ttagttcaga ataaaacaat tcacagtcta + 47401 aatcttttcg cacttgatcg aatatttctt taaaaatggc aacctgagcc attggtaaaa + 47461 ccttccatgt gatacgaggg cgcgtagttt gcattatcgt ttttatcgtt tcaatctggt + 47521 ctgacctcct tgtgttttgt tgatgattta tgtcaaatat taggaatgtt ttcacttaat + 47581 agtattggtt gcgtaacaaa gtgcggtcct gctggcattc tggagggaaa tacaaccgac + 47641 agatgtatgt aaggccaacg tgctcaaatc ttcatacaga aagatttgaa gtaatatttt + 47701 aaccgctaga tgaagagcaa gcgcatggag cgacaaaatg aataaagaac aatctgctga + 47761 tgatccctcc gtggatctga ttcgtgtaaa aaatatgctt aatagcacca tttctatgag + 47821 ttaccctgat gttgtaattg catgtataga acataaggtg tctctggaag cattcagagc + 47881 aattgaggca gcgttggtga agcacgataa taatatgaag gattattccc tggtggttga + 47941 ctgatcacca taactgctaa tcattcaaac tatttagtct gtgacagagc caacacgcag + 48001 tctgtcactg tcaggaaagt ggtaaaactg caactcaatt actgcaatgc cctcgtaatt + 48061 aagtgaattt acaatatcgt cctgttcgga gggaagaacg cgggatgttc attcttcatc + 48121 acttttaatt gatgtatatg ctctcttttc tgacgttagt ctccgacggc aggcttcaat + 48181 gacccaggct gagaaattcc cggacccttt ttgctcaaga gcgatgttaa tttgttcaat + 48241 catttggtta ggaaagcgga tgttgcgggt tgttgttctg cgggttctgt tcttcgttga + 48301 catgaggttg ccccgtattc agtgtcgctg atttgtattg tctgaagttg tttttacgtt + 48361 aagttgatgc agatcaatta atacgatacc tgcgtcataa ttgattattt gacgtggttt + 48421 gatggcctcc acgcacgttg tgatatgtag atgataatca ttatcacttt acgggtcctt + 48481 tccggtgatc cgacaggtta cg +// + diff --git a/tests/lambda_polymorphism_no_junction/expected.gd b/tests/lambda_polymorphism_no_junction_bad_orfs/expected.gd similarity index 84% rename from tests/lambda_polymorphism_no_junction/expected.gd rename to tests/lambda_polymorphism_no_junction_bad_orfs/expected.gd index 741478cb..fe53aef7 100644 --- a/tests/lambda_polymorphism_no_junction/expected.gd +++ b/tests/lambda_polymorphism_no_junction_bad_orfs/expected.gd @@ -1,7 +1,7 @@ #=GENOME_DIFF 1.0 -#=COMMAND ./src/c/breseq/breseq -j 4 -p --no-junction-prediction -o tests/lambda_polymorphism_no_junction -r tests/lambda_polymorphism_no_junction/../data/lambda/lambda.gbk tests/lambda_polymorphism_no_junction/../data/lambda/lambda_mixed_population.fastq -#=REFSEQ tests/lambda_polymorphism_no_junction/../data/lambda/lambda.gbk -#=READSEQ tests/lambda_polymorphism_no_junction/../data/lambda/lambda_mixed_population.fastq +#=COMMAND ./src/c/breseq/breseq -j 4 -p --no-junction-prediction -o tests/lambda_polymorphism_no_junction_bad_orfs -r tests/lambda_polymorphism_no_junction_bad_orfs/../data/lambda/lambda_bad_orfs.gbk tests/lambda_polymorphism_no_junction_bad_orfs/../data/lambda/lambda_mixed_population.fastq +#=REFSEQ tests/lambda_polymorphism_no_junction_bad_orfs/../data/lambda/lambda_bad_orfs.gbk +#=READSEQ tests/lambda_polymorphism_no_junction_bad_orfs/../data/lambda/lambda_mixed_population.fastq #=CONVERTED-BASES 6998495 #=CONVERTED-READS 199957 #=INPUT-BASES 7000144 @@ -22,9 +22,9 @@ SNP 11 73 NC_001416 46136 C aa_new_seq=R aa_position=57 aa_ref_seq=R codon_new_s SNP 12 74 NC_001416 46154 A aa_new_seq=A aa_position=63 aa_ref_seq=A codon_new_seq=GCA codon_number=63 codon_position=3 codon_ref_seq=GCG frequency=8.37693214e-02 gene_name=Rz gene_position=189 gene_product=cell lysis protein gene_strand=> genes_overlapping=Rz locus_tag=lambdap76 locus_tags_overlapping=lambdap76 mutation_category=snp_synonymous position_end=46154 position_start=46154 ref_seq=G snp_type=synonymous transl_table=11 SNP 13 75 NC_001416 46157 G aa_new_seq=L aa_position=64 aa_ref_seq=L codon_new_seq=CTG codon_number=64 codon_position=3 codon_ref_seq=CTC frequency=6.58683777e-02 gene_name=Rz gene_position=192 gene_product=cell lysis protein gene_strand=> genes_overlapping=Rz locus_tag=lambdap76 locus_tags_overlapping=lambdap76 mutation_category=snp_synonymous position_end=46157 position_start=46157 ref_seq=C snp_type=synonymous transl_table=11 SNP 14 76 NC_001416 46162 A aa_new_seq=E aa_position=66 aa_ref_seq=A codon_new_seq=GAA codon_number=66 codon_position=2 codon_ref_seq=GCA frequency=5.47623634e-02 gene_name=Rz gene_position=197 gene_product=cell lysis protein gene_strand=> genes_overlapping=Rz locus_tag=lambdap76 locus_tags_overlapping=lambdap76 mutation_category=snp_nonsynonymous position_end=46162 position_start=46162 ref_seq=C snp_type=nonsynonymous transl_table=11 -SNP 15 77 NC_001416 46185 A aa_new_seq=N aa_position=74 aa_ref_seq=D codon_new_seq=AAT codon_number=74 codon_position=1 codon_ref_seq=GAT frequency=8.79626274e-02 gene_name=Rz gene_position=220 gene_product=cell lysis protein gene_strand=> genes_overlapping=Rz locus_tag=lambdap76 locus_tags_overlapping=lambdap76 mutation_category=snp_nonsynonymous position_end=46185 position_start=46185 ref_seq=G snp_type=nonsynonymous transl_table=11 -SNP 16 78 NC_001416 46190 G aa_new_seq=A|R aa_position=75|2 aa_ref_seq=A|L codon_new_seq=GCG|CGA codon_number=75|2 codon_position=3|2 codon_ref_seq=GCT|CTA frequency=1.09992981e-01 gene_name=Rz|Rz1 gene_position=225|5 gene_product=cell lysis protein|Rz1 protein gene_strand=>|> genes_overlapping=Rz,Rz1 locus_tag=lambdap76|lambdap91 locus_tags_overlapping=lambdap76,lambdap91 mutation_category=snp_synonymous|nonsynonymous position_end=46190 position_start=46190 ref_seq=T snp_type=synonymous|nonsynonymous transl_table=11|11 -SNP 17 79 NC_001416 46430 C frequency=2.39752293e-01 gene_name=Rz/bor gene_position=intergenic (+3/+29) gene_product=cell lysis protein/Bor protein precursor gene_strand=>/< genes_promoter=bor locus_tag=lambdap76/lambdap77 locus_tags_promoter=lambdap77 mutation_category=snp_intergenic position_end=46430 position_start=46430 ref_seq=T snp_type=intergenic +SNP 15 77 NC_001416 46185 A aa_position=74 codon_number=74 codon_position=1 codon_ref_seq=G frequency=8.79626274e-02 gene_name=Rz gene_position=coding (220/220 nt) gene_product=cell lysis protein gene_strand=> genes_overlapping=Rz locus_tag=lambdap76 locus_tags_overlapping=lambdap76 mutation_category=snp_ position_end=46185 position_start=46185 ref_seq=G +SNP 16 78 NC_001416 46190 G aa_new_seq=R aa_position=2 aa_ref_seq=L codon_new_seq=CGA codon_number=2 codon_position=2 codon_ref_seq=CTA frequency=1.09992981e-01 gene_name=Rz1 gene_position=5 gene_product=Rz1 protein gene_strand=> genes_overlapping=Rz1 locus_tag=lambdap91 locus_tags_overlapping=lambdap91 mutation_category=snp_nonsynonymous position_end=46190 position_start=46190 ref_seq=T snp_type=nonsynonymous transl_table=11 +SNP 17 79 NC_001416 46430 C frequency=2.39752293e-01 gene_name=Rz1/bor gene_position=intergenic (+62/+29) gene_product=Rz1 protein/Bor protein precursor gene_strand=>/< genes_promoter=bor locus_tag=lambdap91/lambdap77 locus_tags_promoter=lambdap77 mutation_category=snp_intergenic position_end=46430 position_start=46430 ref_seq=T snp_type=intergenic SNP 18 80 NC_001416 46597 A aa_new_seq=V aa_position=52 aa_ref_seq=V codon_new_seq=GTT codon_number=52 codon_position=3 codon_ref_seq=GTC frequency=2.60710716e-01 gene_name=bor gene_position=156 gene_product=Bor protein precursor gene_strand=< genes_overlapping=bor locus_tag=lambdap77 locus_tags_overlapping=lambdap77 mutation_category=snp_synonymous position_end=46597 position_start=46597 ref_seq=G snp_type=synonymous transl_table=11 SNP 19 81 NC_001416 46608 C aa_new_seq=E aa_position=49 aa_ref_seq=K codon_new_seq=GAG codon_number=49 codon_position=1 codon_ref_seq=AAG frequency=2.11139202e-01 gene_name=bor gene_position=145 gene_product=Bor protein precursor gene_strand=< genes_overlapping=bor locus_tag=lambdap77 locus_tags_overlapping=lambdap77 mutation_category=snp_nonsynonymous position_end=46608 position_start=46608 ref_seq=T snp_type=nonsynonymous transl_table=11 SNP 20 82 NC_001416 46612 T aa_new_seq=G aa_position=47 aa_ref_seq=G codon_new_seq=GGA codon_number=47 codon_position=3 codon_ref_seq=GGG frequency=2.08796501e-01 gene_name=bor gene_position=141 gene_product=Bor protein precursor gene_strand=< genes_overlapping=bor locus_tag=lambdap77 locus_tags_overlapping=lambdap77 mutation_category=snp_synonymous position_end=46612 position_start=46612 ref_seq=C snp_type=synonymous transl_table=11 @@ -36,16 +36,16 @@ SNP 25 87 NC_001416 46679 C aa_new_seq=R aa_position=25 aa_ref_seq=Q codon_new_s SNP 26 88 NC_001416 46680 C aa_new_seq=E aa_position=25 aa_ref_seq=Q codon_new_seq=GAA codon_number=25 codon_position=1 codon_ref_seq=CAA frequency=1.12892151e-01 gene_name=bor gene_position=73 gene_product=Bor protein precursor gene_strand=< genes_overlapping=bor locus_tag=lambdap77 locus_tags_overlapping=lambdap77 multiple_polymorphic_SNPs_in_same_codon=1 mutation_category=snp_nonsynonymous position_end=46680 position_start=46680 ref_seq=G snp_type=nonsynonymous transl_table=11 SNP 27 89 NC_001416 46719 T aa_new_seq=M aa_position=12 aa_ref_seq=L codon_new_seq=ATG codon_number=12 codon_position=1 codon_ref_seq=CTG frequency=6.75792694e-02 gene_name=bor gene_position=34 gene_product=Bor protein precursor gene_strand=< genes_overlapping=bor locus_tag=lambdap77 locus_tags_overlapping=lambdap77 mutation_category=snp_nonsynonymous position_end=46719 position_start=46719 ref_seq=G snp_type=nonsynonymous transl_table=11 SNP 28 90 NC_001416 46720 T aa_new_seq=A aa_position=11 aa_ref_seq=A codon_new_seq=GCA codon_number=11 codon_position=3 codon_ref_seq=GCC frequency=6.63089752e-02 gene_name=bor gene_position=33 gene_product=Bor protein precursor gene_strand=< genes_overlapping=bor locus_tag=lambdap77 locus_tags_overlapping=lambdap77 mutation_category=snp_synonymous position_end=46720 position_start=46720 ref_seq=G snp_type=synonymous transl_table=11 -SNP 29 91 NC_001416 46935 T frequency=7.10277557e-02 gene_name=bor/lambdap78 gene_position=intergenic (-183/+107) gene_product=Bor protein precursor/putative envelope protein gene_strand= ks_quality_p_value=0.892012 locus_tag=lambdap76 major_base=G major_cov=93/48 major_frequency=9.16230679e-01 minor_base=A minor_cov=9/4 new_cov=9/4 new_seq=A polymorphism_frequency=8.37693214e-02 polymorphism_score=17.2 prediction=polymorphism ref_cov=93/48 ref_seq=G snp_type=synonymous total_cov=103/52 transl_table=11 RA 75 . NC_001416 46157 0 C G aa_new_seq=L aa_position=64 aa_ref_seq=L bias_e_value=46586.6 bias_p_value=0.96051 codon_new_seq=CTG codon_number=64 codon_position=3 codon_ref_seq=CTC consensus_score=426.7 fisher_strand_p_value=1 frequency=6.58683777e-02 gene_name=Rz gene_position=192 gene_product=cell lysis protein gene_strand=> ks_quality_p_value=0.732463 locus_tag=lambdap76 major_base=C major_cov=108/50 major_frequency=9.34131622e-01 minor_base=G minor_cov=8/4 new_cov=8/4 new_seq=G polymorphism_frequency=6.58683777e-02 polymorphism_score=20.7 prediction=polymorphism ref_cov=108/50 ref_seq=C snp_type=synonymous total_cov=116/54 transl_table=11 RA 76 . NC_001416 46162 0 C A aa_new_seq=E aa_position=66 aa_ref_seq=A bias_e_value=36374.6 bias_p_value=0.749961 codon_new_seq=GAA codon_number=66 codon_position=2 codon_ref_seq=GCA consensus_score=468.9 fisher_strand_p_value=0.745188 frequency=5.47623634e-02 gene_name=Rz gene_position=197 gene_product=cell lysis protein gene_strand=> ks_quality_p_value=0.513109 locus_tag=lambdap76 major_base=C major_cov=112/61 major_frequency=9.45237637e-01 minor_base=A minor_cov=6/4 new_cov=6/4 new_seq=A polymorphism_frequency=5.47623634e-02 polymorphism_score=16.2 prediction=polymorphism ref_cov=112/61 ref_seq=C snp_type=nonsynonymous total_cov=118/65 transl_table=11 -RA 77 . NC_001416 46185 0 G A aa_new_seq=N aa_position=74 aa_ref_seq=D bias_e_value=19286.8 bias_p_value=0.397649 codon_new_seq=AAT codon_number=74 codon_position=1 codon_ref_seq=GAT consensus_score=450.2 fisher_strand_p_value=0.610315 frequency=8.79626274e-02 gene_name=Rz gene_position=220 gene_product=cell lysis protein gene_strand=> ks_quality_p_value=0.214953 locus_tag=lambdap76 major_base=G major_cov=98/76 major_frequency=9.12037373e-01 minor_base=A minor_cov=8/9 new_cov=8/9 new_seq=A polymorphism_frequency=8.79626274e-02 polymorphism_score=24.4 prediction=polymorphism ref_cov=98/76 ref_seq=G snp_type=nonsynonymous total_cov=106/86 transl_table=11 -RA 78 . NC_001416 46190 0 T G aa_new_seq=A|R aa_position=75|2 aa_ref_seq=A|L bias_e_value=21699.7 bias_p_value=0.447399 codon_new_seq=GCG|CGA codon_number=75|2 codon_position=3|2 codon_ref_seq=GCT|CTA consensus_score=505.1 fisher_strand_p_value=0.353886 frequency=1.09992981e-01 gene_name=Rz|Rz1 gene_position=225|5 gene_product=cell lysis protein|Rz1 protein gene_strand=>|> ks_quality_p_value=0.443206 locus_tag=lambdap76|lambdap91 major_base=T major_cov=92/74 major_frequency=8.90007019e-01 minor_base=G minor_cov=9/12 new_cov=9/12 new_seq=G polymorphism_frequency=1.09992981e-01 polymorphism_score=33.3 prediction=polymorphism ref_cov=92/74 ref_seq=T snp_type=synonymous|nonsynonymous total_cov=102/86 transl_table=11|11 -RA 79 . NC_001416 46430 0 T C bias_e_value=911.861 bias_p_value=0.0188005 consensus_score=141.1 fisher_strand_p_value=0.00501725 frequency=2.39752293e-01 gene_name=Rz/bor gene_position=intergenic (+3/+29) gene_product=cell lysis protein/Bor protein precursor gene_strand=>/< ks_quality_p_value=0.542572 locus_tag=lambdap76/lambdap77 major_base=T major_cov=29/37 major_frequency=7.60247707e-01 minor_base=C minor_cov=17/4 new_cov=17/4 polymorphism_frequency=2.39752293e-01 polymorphism_score=32.2 prediction=polymorphism ref_cov=29/37 snp_type=intergenic total_cov=46/41 +RA 77 . NC_001416 46185 0 G A aa_position=74 bias_e_value=19286.8 bias_p_value=0.397649 codon_number=74 codon_position=1 codon_ref_seq=G consensus_score=450.2 fisher_strand_p_value=0.610315 frequency=8.79626274e-02 gene_name=Rz gene_position=coding (220/220 nt) gene_product=cell lysis protein gene_strand=> ks_quality_p_value=0.214953 locus_tag=lambdap76 major_base=G major_cov=98/76 major_frequency=9.12037373e-01 minor_base=A minor_cov=8/9 new_cov=8/9 new_seq=A polymorphism_frequency=8.79626274e-02 polymorphism_score=24.4 prediction=polymorphism ref_cov=98/76 ref_seq=G total_cov=106/86 +RA 78 . NC_001416 46190 0 T G aa_new_seq=R aa_position=2 aa_ref_seq=L bias_e_value=21699.7 bias_p_value=0.447399 codon_new_seq=CGA codon_number=2 codon_position=2 codon_ref_seq=CTA consensus_score=505.1 fisher_strand_p_value=0.353886 frequency=1.09992981e-01 gene_name=Rz1 gene_position=5 gene_product=Rz1 protein gene_strand=> ks_quality_p_value=0.443206 locus_tag=lambdap91 major_base=T major_cov=92/74 major_frequency=8.90007019e-01 minor_base=G minor_cov=9/12 new_cov=9/12 new_seq=G polymorphism_frequency=1.09992981e-01 polymorphism_score=33.3 prediction=polymorphism ref_cov=92/74 ref_seq=T snp_type=nonsynonymous total_cov=102/86 transl_table=11 +RA 79 . NC_001416 46430 0 T C bias_e_value=911.861 bias_p_value=0.0188005 consensus_score=141.1 fisher_strand_p_value=0.00501725 frequency=2.39752293e-01 gene_name=Rz1/bor gene_position=intergenic (+62/+29) gene_product=Rz1 protein/Bor protein precursor gene_strand=>/< ks_quality_p_value=0.542572 locus_tag=lambdap91/lambdap77 major_base=T major_cov=29/37 major_frequency=7.60247707e-01 minor_base=C minor_cov=17/4 new_cov=17/4 polymorphism_frequency=2.39752293e-01 polymorphism_score=32.2 prediction=polymorphism ref_cov=29/37 snp_type=intergenic total_cov=46/41 RA 80 . NC_001416 46597 0 G A aa_new_seq=V aa_position=52 aa_ref_seq=V bias_e_value=3262.13 bias_p_value=0.0672576 codon_new_seq=GTT codon_number=52 codon_position=3 codon_ref_seq=GTC consensus_score=115.9 fisher_strand_p_value=0.140556 frequency=2.60710716e-01 gene_name=bor gene_position=156 gene_product=Bor protein precursor gene_strand=< ks_quality_p_value=0.0889035 locus_tag=lambdap77 major_base=G major_cov=41/24 major_frequency=7.39289284e-01 minor_base=A minor_cov=10/13 new_cov=10/13 new_seq=A polymorphism_frequency=2.60710716e-01 polymorphism_score=44.5 prediction=polymorphism ref_cov=41/24 ref_seq=G snp_type=synonymous total_cov=51/38 transl_table=11 RA 81 . NC_001416 46608 0 T C aa_new_seq=E aa_position=49 aa_ref_seq=K bias_e_value=17253.5 bias_p_value=0.355728 codon_new_seq=GAG codon_number=49 codon_position=1 codon_ref_seq=AAG consensus_score=127.4 fisher_strand_p_value=0.150732 frequency=2.11139202e-01 gene_name=bor gene_position=145 gene_product=Bor protein precursor gene_strand=< ks_quality_p_value=0.738595 locus_tag=lambdap77 major_base=T major_cov=38/20 major_frequency=7.88860798e-01 minor_base=C minor_cov=7/9 new_cov=7/9 new_seq=C polymorphism_frequency=2.11139202e-01 polymorphism_score=25.2 prediction=polymorphism ref_cov=38/20 ref_seq=T snp_type=nonsynonymous total_cov=45/29 transl_table=11 RA 82 . NC_001416 46612 0 C T aa_new_seq=G aa_position=47 aa_ref_seq=G bias_e_value=31755 bias_p_value=0.654715 codon_new_seq=GGA codon_number=47 codon_position=3 codon_ref_seq=GGG consensus_score=129.1 fisher_strand_p_value=0.436944 frequency=2.08796501e-01 gene_name=bor gene_position=141 gene_product=Bor protein precursor gene_strand=< ks_quality_p_value=0.674367 locus_tag=lambdap77 major_base=C major_cov=41/27 major_frequency=7.91203499e-01 minor_base=T minor_cov=9/9 new_cov=9/9 new_seq=T polymorphism_frequency=2.08796501e-01 polymorphism_score=32.3 prediction=polymorphism ref_cov=41/27 ref_seq=C snp_type=synonymous total_cov=50/36 transl_table=11 @@ -98,16 +98,16 @@ RA 87 . NC_001416 46679 0 T C aa_new_seq=R aa_position=25 aa_ref_seq=Q bias_e_va RA 88 . NC_001416 46680 0 G C aa_new_seq=E aa_position=25 aa_ref_seq=Q bias_e_value=23899.4 bias_p_value=0.492751 codon_new_seq=GAA codon_number=25 codon_position=1 codon_ref_seq=CAA consensus_score=329.6 fisher_strand_p_value=0.182485 frequency=1.12892151e-01 gene_name=bor gene_position=73 gene_product=Bor protein precursor gene_strand=< ks_quality_p_value=0.999494 locus_tag=lambdap77 major_base=G major_cov=57/61 major_frequency=8.87107849e-01 minor_base=C minor_cov=11/5 multiple_polymorphic_SNPs_in_same_codon=1 new_cov=11/5 new_seq=C polymorphism_frequency=1.12892151e-01 polymorphism_score=24.5 prediction=polymorphism ref_cov=57/61 ref_seq=G snp_type=nonsynonymous total_cov=68/66 transl_table=11 RA 89 . NC_001416 46719 0 G T aa_new_seq=M aa_position=12 aa_ref_seq=L bias_e_value=44000.6 bias_p_value=0.907191 codon_new_seq=ATG codon_number=12 codon_position=1 codon_ref_seq=CTG consensus_score=264.3 fisher_strand_p_value=1 frequency=6.75792694e-02 gene_name=bor gene_position=34 gene_product=Bor protein precursor gene_strand=< ks_quality_p_value=0.601365 locus_tag=lambdap77 major_base=G major_cov=39/58 major_frequency=9.32420731e-01 minor_base=T minor_cov=3/4 new_cov=3/4 new_seq=T polymorphism_frequency=6.75792694e-02 polymorphism_score=11.8 prediction=polymorphism ref_cov=39/58 ref_seq=G snp_type=nonsynonymous total_cov=43/62 transl_table=11 RA 90 . NC_001416 46720 0 G T aa_new_seq=A aa_position=11 aa_ref_seq=A bias_e_value=42920 bias_p_value=0.884912 codon_new_seq=GCA codon_number=11 codon_position=3 codon_ref_seq=GCC consensus_score=266.4 fisher_strand_p_value=1 frequency=6.63089752e-02 gene_name=bor gene_position=33 gene_product=Bor protein precursor gene_strand=< ks_quality_p_value=0.560369 locus_tag=lambdap77 major_base=G major_cov=39/58 major_frequency=9.33691025e-01 minor_base=T minor_cov=3/4 new_cov=3/4 new_seq=T polymorphism_frequency=6.63089752e-02 polymorphism_score=10.3 prediction=polymorphism ref_cov=39/58 ref_seq=G snp_type=synonymous total_cov=42/62 transl_table=11 -RA 91 . NC_001416 46935 0 G T bias_e_value=48501.8 bias_p_value=0.999996 consensus_score=163.5 fisher_strand_p_value=1 frequency=7.10277557e-02 gene_name=bor/lambdap78 gene_position=intergenic (-183/+107) gene_product=Bor protein precursor/putative envelope protein gene_strand=