DESCRIPTION

Package: TAPseq
Type: Package
Title: Targeted scRNA-seq primer design for TAP-seq
Version: 1.15.2
Authors@R: c(
  person(c("Andreas", "R."), "Gschwind", email = "andreas.gschwind@stanford.edu",
         role = c("aut", "cre"), comment = c(ORCID = "0000-0002-0769-6907")),
  person("Lars", "Velten", email = "lars.velten@crg.eu", role = "aut",
         comment = c(ORCID = "0000-0002-1233-5874")),
  person(c("Lars", "M."), "Steinmetz", role = "aut"))
Description: Design primers for targeted single-cell RNA-seq used by TAP-seq. Create sequence
  templates for target gene panels and design gene-specific primers using Primer3. Potential
  off-targets can be estimated with BLAST. Requires working installations of Primer3 and BLASTn.
URL: https://github.com/argschwind/TAPseq
BugRepots: https://github.com/argschwind/TAPseq/issues
biocViews: SingleCell, Sequencing, Technology, CRISPR, PooledScreens
License: MIT + file LICENSE
Encoding: UTF-8
RoxygenNote: 7.2.3
VignetteBuilder: knitr
Depends: R (>= 4.0.0)
Imports:
    methods,
    GenomicAlignments,
    GenomicRanges,
    IRanges,
    BiocGenerics,
    S4Vectors (>= 0.20.1),
    GenomeInfoDb,
    BSgenome,
    GenomicFeatures,
    Biostrings,
    dplyr,
    tidyr,
    BiocParallel
Suggests: 
    testthat,
    BSgenome.Hsapiens.UCSC.hg38,
    knitr,
    rmarkdown,
    ggplot2,
    Seurat,
    glmnet,
    cowplot,
    Matrix,
    rtracklayer,
    BiocStyle
SystemRequirements:
    Primer3 (>= 2.5.0),
    BLAST+ (>=2.6.0)