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When providing metaMiner with an un-annotated nucleotide FASTA file that has more than one DNA sequences, transeq is run to six-frame translate it prior to running hmmsearch.
However, in my experience transeq.py only outputs six-frame translations for the first sequence in the FASTA file. This can be reproduced as follows:
Use gotranseq as a near drop-in replacement as it requires only a single binary, compared with the transeq program within EMBOSS.
Caveat: the output is not in --wide format and wraps at 60 characters, with no --wide option available.
The text was updated successfully, but these errors were encountered:
Description
When providing metaMiner with an un-annotated nucleotide FASTA file that has more than one DNA sequences, transeq is run to six-frame translate it prior to running hmmsearch.
However, in my experience
transeq.py
only outputs six-frame translations for the first sequence in the FASTA file. This can be reproduced as follows:Now,
test.out
contains:test.out
should contain:Possible solutions
Use gotranseq as a near drop-in replacement as it requires only a single binary, compared with the
transeq
program within EMBOSS.Caveat: the output is not in
--wide
format and wraps at 60 characters, with no--wide
option available.The text was updated successfully, but these errors were encountered: