We read every piece of feedback, and take your input very seriously.
To see all available qualifiers, see our documentation.
Have a question about this project? Sign up for a free GitHub account to open an issue and contact its maintainers and the community.
By clicking “Sign up for GitHub”, you agree to our terms of service and privacy statement. We’ll occasionally send you account related emails.
Already on GitHub? Sign in to your account
When I executed the Simulated data from scRNA data code of the SimulatedData.ipynb file, the following error occurred:
OSError Traceback (most recent call last) Cell In[7], line 4 1 scRNADir1 = 'Brain_10X' 2 scRNADir2 = 'Brain_Smart-seq' ----> 4 generate_dataset_data(scRNADir1, scRNADir2, celltype_key = 'celltype_final',reverse = False) 5 generate_dataset_data(scRNADir1, scRNADir2, celltype_key = 'celltype_final',reverse = True) Cell In[5], line 2, in generate_dataset_data(scRNADir1, scRNADir2, celltype_key, reverse) 1 def generate_dataset_data(scRNADir1, scRNADir2, celltype_key,reverse = False): ----> 2 scrna, spatial = check_dataset(scRNADir1, scRNADir2, top_genes=200, plot=False,reverse=reverse,check=False) 3 if not reverse: 4 output_dir = scRNADir1 Cell In[3], line 37, in check_dataset(scRNADir1, scRNADir2, top_genes, plot, reverse, check) 34 assert len(celltype_meta_1['celltype_final'][celltype_meta_1['celltype_final'] != 'Not_specific'].unique()) == len(overlaped_celltype), data_ident_1+': unique celltype length dont match.' 35 assert len(celltype_meta_2['celltype_final'][celltype_meta_2['celltype_final'] != 'Not_specific'].unique()) == len(overlaped_celltype), data_ident_2+': unique celltype length dont match.' ---> 37 data_1 = sc.read_h5ad(scRNADir1 + '/scRNA.h5ad') 38 data_2 = sc.read_h5ad(scRNADir2 + '/scRNA.h5ad') 40 data_1 = check_adata(data_1, os.path.join(scRNADir1, 'Processed_scRNA.h5ad')) File ~/miniconda3/envs/py38/lib/python3.8/site-packages/anndata/_io/h5ad.py:219, in read_h5ad(filename, backed, as_sparse, as_sparse_fmt, chunk_size) 211 raise NotImplementedError( 212 "Currently only `X` and `raw/X` can be read as sparse." 213 ) 215 rdasp = partial( 216 read_dense_as_sparse, sparse_format=as_sparse_fmt, axis_chunk=chunk_size 217 ) --> 219 with h5py.File(filename, "r") as f: 221 def callback(func, elem_name: str, elem, iospec): 222 if iospec.encoding_type == "anndata" or elem_name.endswith("/"): File ~/miniconda3/envs/py38/lib/python3.8/site-packages/h5py/_hl/files.py:424, in File.__init__(self, name, mode, driver, libver, userblock_size, swmr, rdcc_nslots, rdcc_nbytes, rdcc_w0, track_order, fs_strategy, fs_persist, fs_threshold, **kwds) 422 with phil: 423 fapl = make_fapl(driver, libver, rdcc_nslots, rdcc_nbytes, rdcc_w0, **kwds) --> 424 fid = make_fid(name, mode, userblock_size, 425 fapl, fcpl=make_fcpl(track_order=track_order, fs_strategy=fs_strategy, 426 fs_persist=fs_persist, fs_threshold=fs_threshold), 427 swmr=swmr) 429 if isinstance(libver, tuple): 430 self._libver = libver File ~/miniconda3/envs/py38/lib/python3.8/site-packages/h5py/_hl/files.py:190, in make_fid(name, mode, userblock_size, fapl, fcpl, swmr) 188 if swmr and swmr_support: 189 flags |= h5f.ACC_SWMR_READ --> 190 fid = h5f.open(name, flags, fapl=fapl) 191 elif mode == 'r+': 192 fid = h5f.open(name, h5f.ACC_RDWR, fapl=fapl) File h5py/_objects.pyx:54, in h5py._objects.with_phil.wrapper() File h5py/_objects.pyx:55, in h5py._objects.with_phil.wrapper() File h5py/h5f.pyx:96, in h5py.h5f.open() OSError: Unable to open file (truncated file: eof = 3001450496, sblock->base_addr = 0, stored_eof = 5047473549)
I searched for similar issues and got the answer that the original file is corrupted. my env:
Package Version ------------------- ------- anndata 0.9.1 asttokens 2.0.5 backcall 0.2.0 blosc2 2.0.0 comm 0.1.2 contourpy 1.0.7 cycler 0.11.0 Cython 0.29.34 debugpy 1.5.1 decorator 5.1.1 dunamai 1.16.0 executing 0.8.3 fonttools 4.39.3 get_version 3.5.4 h5py 3.1.0 importlib-metadata 6.0.0 importlib-resources 5.12.0 ipykernel 6.19.2 ipython 8.12.0 jedi 0.18.1 joblib 1.2.0 jupyter_client 8.1.0 jupyter_core 5.3.0 kiwisolver 1.4.4 legacy-api-wrap 1.2 llvmlite 0.40.0 matplotlib 3.3.4 matplotlib-inline 0.1.6 msgpack 1.0.5 natsort 8.3.1 nest-asyncio 1.5.6 networkx 3.1 numba 0.57.0 numexpr 2.8.4 numpy 1.24.3 packaging 23.0 pandas 1.1.5 parso 0.8.3 patsy 0.5.3 pexpect 4.8.0 pickleshare 0.7.5 Pillow 9.5.0 pip 23.0.1 platformdirs 2.5.2 prompt-toolkit 3.0.36 psutil 5.9.0 ptyprocess 0.7.0 pure-eval 0.2.2 py-cpuinfo 9.0.0 Pygments 2.15.1 pynndescent 0.5.10 pyparsing 3.0.9 python-dateutil 2.8.2 pytz 2023.3 pyzmq 25.0.2 scanpy 1.7.2 scikit-learn 1.2.2 scipy 1.10.1 seaborn 0.12.2 setuptools 66.0.0 sinfo 0.3.4 six 1.16.0 stack-data 0.2.0 statsmodels 0.14.0 stdlib-list 0.8.0 tables 3.8.0 threadpoolctl 3.1.0 tornado 6.2 tqdm 4.65.0 traitlets 5.7.1 typing_extensions 4.5.0 tzdata 2023.3 umap-learn 0.5.3 wcwidth 0.2.5 wheel 0.38.4 zipp 3.11.0
The text was updated successfully, but these errors were encountered:
No branches or pull requests
When I executed the Simulated data from scRNA data code of the SimulatedData.ipynb file, the following error occurred:
I searched for similar issues and got the answer that the original file is corrupted.
my env:
The text was updated successfully, but these errors were encountered: