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DESCRIPTION
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Package: recountWorkflow
Title: recount workflow: accessing over 70,000 human RNA-seq samples with
Bioconductor
Version: 1.31.0
Date: 2024-05-21
Authors@R: c(person("Leonardo", "Collado-Torres", role = c("aut", "cre"),
email = "[email protected]"), person("Abhinav", "Nellore", role = "ctb",
email = "[email protected]"), person(c("Andrew", "E."), "Jaffe",
role = "ctb", email = "[email protected]"))
Description: The recount2 resource is composed of over 70,000 uniformly
processed human RNA-seq samples spanning TCGA and SRA, including GTEx. The
processed data can be accessed via the recount2 website and the recount
Bioconductor package. This workflow explains in detail how to use the
recount package and how to integrate it with other Bioconductor packages for
several analyses that can be carried out with the recount2 resource. In
particular, we describe how the coverage count matrices were computed in
recount2 as well as different ways of obtaining public metadata, which can
facilitate downstream analyses. Step-by-step directions show how to do a
gene level differential expression analysis, visualize base-level genome
coverage data, and perform an analyses at multiple feature levels. This
workflow thus provides further information to understand the data in
recount2 and a compendium of R code to use the data.
Depends: R (>= 3.6.0)
License: Artistic-2.0
Encoding: UTF-8
biocViews: Workflow, ResourceQueryingWorkflow
VignetteBuilder: knitr
Imports:
recount,
GenomicRanges,
limma,
edgeR,
DESeq2,
pheatmap,
regionReport,
clusterProfiler,
org.Hs.eg.db,
gplots,
derfinder,
GenomicState,
bumphunter,
derfinderPlot
Suggests:
BiocStyle,
BiocWorkflowTools,
knitr,
magick,
sessioninfo,
rmarkdown
NeedsCompilation: no
Workflow: true
URL: https://github.com/LieberInstitute/recountWorkflow
BugReports: https://support.bioconductor.org/t/recountWorkflow/
RoxygenNote: 7.2.3