Using PCRCalc

This guide assume you know what a Polimerase Chain Reaction (PCR) is, and will recognize the terms presented by the application. It is not intended to teach you PCR principles and application.

PCRCalc is a Graphical User Interface (GUI), when you execute it, a single window will be presented to you with all data needed to calculate a PCR Mixture of a given number of reactions. PCRCalc accompanies a small thermocycler minimum run time calculator that is not directly related to the main calculator and do not have to be used if not needed (it will be documented later).

The fist column presents reagents stock values, changes to these values are disabled initially but can be enabled by checking the Edit stock values check button. Then you will be able to alter these values accordingly to you stock concentrations. If you want a more permanent change, alter the default values imported from stock.json file in the folder presets installed along with PCRCalc, change this file values as you wish, but be careful to not corrupt the file.

The second column shows PCR concentration values and can be edit by default, if you want to lock these values, uncheck the Edit PCR values check button. The defaut values are hard coded, an will always be presented on application statup. Alter them accordingly to your needs.

Once stock and PCR concentrations are set, just define the reaction number and volume at the bottom part of the third column and click the View Mix button.

This window will popup: