Automate primer design

[hochshi]

We should provide the user with a complete list of oligo to order and primers

[jonathaw]

primers are very hard to design, and there is no one way to do that.
we can easily tell a user what he needs to add to the ends of primers for the GG reaction, and what they need to amplify the variable oligos.

[hochshi]

Apparently primer design is not as hard as thought.
See: https://pydna.readthedocs.io/index.html#pydna.design.primer_design
And https://pydna.readthedocs.io/index.html#pydna.tm.tmstaluc98 for TM calculation

[hochshi]

Ravit: Design the primers to have a Tm of at least 60 C and they should end (in the 3') with either C or G.

Input from user should be:

1. Temperature
2. Temperature restriction, (above or below)
3. End base? (3')
4. Length restriction? - Ask user, (Ravit: as short as possible)