diff --git a/scRNA-seq/exercise_01-scrna_quant.Rmd b/scRNA-seq/exercise_01-scrna_quant.Rmd index 1e66ee77..a54a73af 100644 --- a/scRNA-seq/exercise_01-scrna_quant.Rmd +++ b/scRNA-seq/exercise_01-scrna_quant.Rmd @@ -16,10 +16,10 @@ In this exercise notebook we will be using [*Tabula Muris* project](https://www. - Part B of this exercise is using the quantified data to conduct cell filtering and normalization of `10X_P7_12`. - Part C of this exercise will introduce you to performing doublet detection using `10X_P7_12`. -You are welcome to [skip to Part B](#part_b:_performing_dimension_reduction_on_10x_p7_12’s_cells) if you are not interested in performing the quantification steps with Alevin. +You are welcome to skip to Part B if you are not interested in performing the quantification steps with Alevin. Do not skip to Part C! You will need to complete Part B before completing Part C. -# Part A: Quantifying single-cell expression of a mammary gland sample. +## Part A: Quantifying single-cell expression of a mammary gland sample. In this part of the exercise we will be following the same steps for a tag-based scRNA-seq sample as we did in the `01-scRNA_quant_qc.Rmd` notebook. @@ -200,7 +200,7 @@ This will take some time; when it is done the last message you will see is [alevinLog] [info] Finished optimizer ``` -## Part B: Performing dimension reduction on 10X_P7_12's cells +## Part B: Performing filtering and normalization on 10X_P7_12 cells In the second half of this exercise notebook, we will use the Alevin quantified data from sample `10X_P7_12` to perform cell filtering and normalization.